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Scanning electron microscope image of a snout beetle
http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html   Image: public domain. Feb. 2012
Calculating Linear Magnification
                         of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar - in mm.


Convert your measurement into µm.

              32mm

Calculate the magnification.
(Magnification = measured length /the number written.
As long as you have the SAME UNITS)

     32 x 1000 = 32000µm


Magnifiation = the number of times bigger the scale bar actually is, compared to the
measurement written on it?




      32000µm / 100 µm = 320x                       magnification
Scanning electron microscope image of a snout beetle
http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html   Image: public domain. Feb. 2012
Calculating Specimen Size
                                        using a scale bar

There are four steps:

Measure the length of the Specimen in mm.
(show working)

Measure the length of the scale bar in mm.


Calculate how many scale bar lengths make the specimen.
(Divide length of specimen by length of scale bar)


Calculate the size. Multiply the scale bar label by the last answer.
(UNITS are the same as the scale bar)




           2.6 x 100 µm = 260µm
Scanning electron microscope image of a snout beetle
              http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html   Image: public domain. Feb. 2012




Magnifiation = x 320
Calculating Specimen Size
                                      using magnification

There are three steps:

Measure the length of the Image of the Specimen in mm.
(show working)


Convert the length of the Specimen image to µm.
(x 1000)


Calculate the actual size.
Divide the length of the specimen image by the magnification
(UNITS are µm)
Electron microscope image of pollen grains from oriental lily.
http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html   Image: public domain. Feb. 2012
Calculating Linear Magnification
                         of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar - in mm.


Convert your measurement into µm.



Calculate the magnification.
(Magnification = measured length /the number written.
As long as you have the SAME UNITS)




Magnifiation = the number of times bigger the scale bar actually is, compared to the
measurement written on it?




                                                    magnification
Electron microscope image of pollen grains from oriental lily.
http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html   Image: public domain. Feb. 2012
Calculating Specimen Size
                                        using a scale bar

There are four steps:

Measure the length of the Specimen in mm.
(show working)

Measure the length of the scale bar in mm.


Calculate how many scale bar lengths make the specimen.
(Divide length of specimen by length of scale bar)


Calculate the size. Multiply the scale bar label by the last answer.
(UNITS are the same as the scale bar)
Electron microscope image of pollen grains from oriental lily.
    http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html   Image: public domain. Feb. 2012




Magnification = x 2500
Calculating Specimen Size
                                      using magnification

There are three steps:

Measure the length of the Image of the Specimen in mm.
(show working)


Convert the length of the Specimen image to µm.
(x 1000)


Calculate the actual size.
Divide the length of the specimen image by the magnification
(UNITS are µm)
Human leukocyte - showing golgi apparatus
http://remf.dartmouth.edu/images/humanBloodCellsTEM/source/3.html Image: public domain. Feb. 2012
Calculating Linear Magnification
                         of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar - in mm.


Convert your measurement into nm this time.



Calculate the magnification.
(Magnification = measured length /the number written.
As long as you have the SAME UNITS)




Magnifiation = the number of times bigger the scale bar actually is, compared to the
measurement written on it?




                                                   magnification
Calculating Specimen Size
                                        using a scale bar

There are four steps:

Measure the length of the Specimen in mm.
(show working)

Measure the length of the scale bar in mm.


Calculate how many scale bar lengths make the specimen.
(Divide length of specimen by length of scale bar)


Calculate the size. Multiply the scale bar label by the last answer.
(UNITS are the same as the scale bar)

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Electron micrograph ultrastructure_magnification

  • 1. Scanning electron microscope image of a snout beetle http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012
  • 2. Calculating Linear Magnification of an Electron-Micrograph using it's scale bar There are three steps: Measure the length of the scale bar - in mm. Convert your measurement into µm. 32mm Calculate the magnification. (Magnification = measured length /the number written. As long as you have the SAME UNITS) 32 x 1000 = 32000µm Magnifiation = the number of times bigger the scale bar actually is, compared to the measurement written on it? 32000µm / 100 µm = 320x magnification
  • 3. Scanning electron microscope image of a snout beetle http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012
  • 4. Calculating Specimen Size using a scale bar There are four steps: Measure the length of the Specimen in mm. (show working) Measure the length of the scale bar in mm. Calculate how many scale bar lengths make the specimen. (Divide length of specimen by length of scale bar) Calculate the size. Multiply the scale bar label by the last answer. (UNITS are the same as the scale bar) 2.6 x 100 µm = 260µm
  • 5. Scanning electron microscope image of a snout beetle http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012 Magnifiation = x 320
  • 6. Calculating Specimen Size using magnification There are three steps: Measure the length of the Image of the Specimen in mm. (show working) Convert the length of the Specimen image to µm. (x 1000) Calculate the actual size. Divide the length of the specimen image by the magnification (UNITS are µm)
  • 7. Electron microscope image of pollen grains from oriental lily. http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012
  • 8. Calculating Linear Magnification of an Electron-Micrograph using it's scale bar There are three steps: Measure the length of the scale bar - in mm. Convert your measurement into µm. Calculate the magnification. (Magnification = measured length /the number written. As long as you have the SAME UNITS) Magnifiation = the number of times bigger the scale bar actually is, compared to the measurement written on it? magnification
  • 9. Electron microscope image of pollen grains from oriental lily. http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012
  • 10. Calculating Specimen Size using a scale bar There are four steps: Measure the length of the Specimen in mm. (show working) Measure the length of the scale bar in mm. Calculate how many scale bar lengths make the specimen. (Divide length of specimen by length of scale bar) Calculate the size. Multiply the scale bar label by the last answer. (UNITS are the same as the scale bar)
  • 11. Electron microscope image of pollen grains from oriental lily. http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012 Magnification = x 2500
  • 12. Calculating Specimen Size using magnification There are three steps: Measure the length of the Image of the Specimen in mm. (show working) Convert the length of the Specimen image to µm. (x 1000) Calculate the actual size. Divide the length of the specimen image by the magnification (UNITS are µm)
  • 13. Human leukocyte - showing golgi apparatus http://remf.dartmouth.edu/images/humanBloodCellsTEM/source/3.html Image: public domain. Feb. 2012
  • 14. Calculating Linear Magnification of an Electron-Micrograph using it's scale bar There are three steps: Measure the length of the scale bar - in mm. Convert your measurement into nm this time. Calculate the magnification. (Magnification = measured length /the number written. As long as you have the SAME UNITS) Magnifiation = the number of times bigger the scale bar actually is, compared to the measurement written on it? magnification
  • 15. Calculating Specimen Size using a scale bar There are four steps: Measure the length of the Specimen in mm. (show working) Measure the length of the scale bar in mm. Calculate how many scale bar lengths make the specimen. (Divide length of specimen by length of scale bar) Calculate the size. Multiply the scale bar label by the last answer. (UNITS are the same as the scale bar)