This document discusses microbial nutrition and growth. It explains that microbes require nutrients for energy, cellular activities, and constructing new cellular components. The main nutrients include carbon, nitrogen, phosphorus, and trace elements. It categorizes microbes based on their carbon and energy sources. It also describes the physical and chemical requirements for microbial growth, including temperature, pH, oxygen levels, and nutrients. It discusses culture media, methods for measuring growth, and techniques for obtaining pure cultures.
Definition of bacterial growth
Modes of multiplication in bacteria
List the salient features of bacterial growth curve.
Concepts of generation time and growth curve
Calculations of generation time
Definition of bacterial growth
Modes of multiplication in bacteria
List the salient features of bacterial growth curve.
Concepts of generation time and growth curve
Calculations of generation time
Growth of bacteria is affected by many factors such as nutrition concentration and other environmental factors.
Some of the important factors affecting bacterial growth are:
Nutrition concentration
Temperature
Gaseous concentration
pH
Ions and salt concentration
Available water
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
What is culture media
Bacteria culture
Importance of culturing.
Culturing and medium.
History of culture media.
How many types of growth media .
Basic components of culture media.
Classification
Consistancy
Nutritional components
Functional use
Aseptic condittion .
General steps for preparation of culture media .
Selective media .
Enrichment media.
Storage of culture media.
Growth of bacteria is affected by many factors such as nutrition concentration and other environmental factors.
Some of the important factors affecting bacterial growth are:
Nutrition concentration
Temperature
Gaseous concentration
pH
Ions and salt concentration
Available water
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
What is culture media
Bacteria culture
Importance of culturing.
Culturing and medium.
History of culture media.
How many types of growth media .
Basic components of culture media.
Classification
Consistancy
Nutritional components
Functional use
Aseptic condittion .
General steps for preparation of culture media .
Selective media .
Enrichment media.
Storage of culture media.
The presentation discusses all about microbial growth, it explains various nutritional and physical requirements of bacteria for growth, it is also illustrated here the standard bacterial growth curve
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
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Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
1. MICROBIAL NUTRITION AND
GROWTH
BY
DR JAWAD NAZIR
ASSISTANT PROFESSOR
DEPARTMENT OF MICROBIOLOGY
UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES, LAHORE
2. Microbial nutrition and growth
Requirement of Nutrition?
To obtain energy
To carry out normal cellular activities
Construct new cellular components
3. Microbial nutrition and growth
Common Nutrients
Macronutrients :
95 % of cell dry mass is made up of
Carbon, oxygen, hydrogen, nitrogen, sulpher, phosphorus
Components of organic molecules
Potassium: required by enzymes (protein synthesis)
Calcium: component of spore
Magnesium : cofactor for enzymes
Iron: cytochrom and electron transport chain
4. Microbial nutrition and growth
Common Nutrients
Trace elements (Micronutrients):
Normally serve as co factor in enzymes
Manganese, Zinc, Cobalt, Molybdenum, nickel, and copper
Required in such small amount that contaminants in
water, glassware or media ingredients are adequate to
fulfill their requirements
Nutrients required per litre of medium:
Major ingredients required in grams
Minor ingredients required in mili grams
Trace elements required in micrograms
5. Microbial nutrition and growth
Categorization of bacteria
Carbon:
Autotroph
Heterotroph
Energy source:
Phototroph
Chemotroph
Hydrogen or electron source:
Lithotroph
Organotrophs
6. Microbial nutrition and growth
Nutritional types of microorganisms
Photoautotrophs:
Who use light as energy source and CO2 as carbon source
Oxygenic in nature
Algae, blue green bacteria, purple and green sulfur
bacteria
Photoheterotrophs:
Who use light as energy source and an organic carbon
source
Common inhabitant of polluted lakes and streams
Purple non sulfur bacteria and green non sulfur bacteria
7. Microbial nutrition and growth
Nutritional types of microorganisms
Chemoautotroph:
Who use inorganic chemicals as energy source and
CO2 as carbon source
Nitrosomonas, Nitrobacter, Hydrogenomonas,
Thiobacillus ferrooxidans
Chemohetrotroph:
Who use organic molecules as a source of carbon and
energy
Same organic molecule satisfy all the requirements
All pathogenic bacteria fall in this category
8. There is no naturally occuring organic
molecule that cannot be utilized by
some microorganisms
10. Microbial nutrition and growth
Growth factors
Organic molecules required for cell structure and
function and can not be synthesized by a specific
microbe is called as growth factor
Amino acids
Nucleotides (purine pyrimidine)
Vitamins
11. Microbial nutrition and growth
Microbial growth?
Microbial growth = increase in number of cells
Not cell size
12. Microbial nutrition and growth
Requirements of growth
Physical requirements:
Temperature
pH
Osmotic pressure
Chemical requirements:
Carbon, nitrogen, sulfr, phosphorus
Trace elements
Oxygen
Organic growth factors
13. Microbial nutrition and growth
Physical Requirements
Temperature:
Minimum growth temperature
Optimum growth temperature
Maximum growth temperature
17. Microbial nutrition and growth
pH:
Most bacteria grow between pH 6.5 and 7.5
Molds and yeasts grow between pH 5 and 6
Acidophiles grow in acidic environments
Physical Requirements
21. Microbial nutrition and growth
Physical Requirements
Osmotic pressure:
Hypertonic environments, increase salt or sugar,
cause plasmolysis
Extreme or obligate halophiles require high
osmotic pressure
Facultative halophiles tolerate high osmotic
pressure
23. Microbial nutrition and growth
Chemical requirements
Carbon:
Structural organic molecules, energy source
Chemoheterotrophs use organic carbon sources
Autotrophs use CO2
24. Microbial nutrition and growth
Chemical requirements
Nitrogen:
In amino acids, proteins
Most bacteria decompose proteins
Some bacteria use ammonium (NH4
+) or Nitrate (NO3
)
A few bacteria use N2 in nitrogen fixation
Sulfur:
In amino acids, thiamine, biotin
Most bacteria decompose proteins
Some bacteria use sulfate (SO4
2) or H2S
Phosphorus:
In DNA, RNA, ATP, and membranes
Phosphate (PO4
3)is a source of phosphorus
25. Microbial nutrition and growth
Chemical requirements
Trace elements:
Inorganic elements required in small amounts
Usually as enzyme cofactors
26. Microbial nutrition and growth
Oxygen (O2)
The Requirements for Growth: Chemical Requirements
obligate
aerobes
Faultative
anaerobes
Obligate
anaerobes
Aerotolerant
anaerobes
Microaerophiles
27. Microbial nutrition and growth
Singlet oxygen: O2 boosted to a higher-energy state
Superoxide free radicals: O2
2
Peroxide anion: O2
2
Hydroxyl radical (OH)
Toxic Forms of Oxygen
28. Microbial nutrition and growth
Organic Growth Factors
Organic compounds obtained from the environment
Vitamins, amino acids, purines, pyrimidines
The Requirements for Growth: Chemical Requirements
29. Microbial nutrition and growth
Culture Medium: Nutrients prepared for microbial
growth
Sterile: No living microbes
Inoculum: Introduction of microbes into medium
Culture: Microbes growing in/on culture medium
Culture Media
31. Microbial nutrition and growth
Complex polysaccharide
Used as solidifying agent for culture media in Petri
plates, slants, and deeps
Generally not metabolized by microbes
Liquefies at 100°C
Solidifies ~40°C
Agar
32. Microbial nutrition and growth
Chemically Defined Media: Exact chemical
composition is known
Complex Media: Extracts and digests of yeasts,
meat, or plants
Nutrient broth
Nutrient agar
Culture Media
34. Microbial nutrition and growth
Selective media
Suppress unwanted microbes and encourage
desired microbes.
MacConkey agar:
Bile salts allow intestinal bcteria
Staph-110 media:
High salts concentration make selective for staph.
Campylobacter selective media:
Polymyxin B, Trimethoprim, Rifamycin
35. Microbial nutrition and growth
Make it easy to distinguish colonies of different microbes.
Differential Media
Lactose fermenter and non-fermenter on MacConkey agar
37. Microbial nutrition and growth
Enriched medium
Used to grow fastidious bacteria
Substances like blood, serum, egg are added to the
basal medium.
Blood agar, Chocolate agar, PPLO agar
38. Microbial nutrition and growth
Encourages growth of desired microbe
Selenite broth used to enhance the growth of
Salmonella species as sodium selenite is toxic for
E.coli and Proteus
Inoculate fecal material in selenite broth
Incubate for 18-24 hours
Culture on selective or enriched media
Enrichment Media
39. Microbial nutrition and growth
Reducing media
Contain chemicals (thioglycollate or oxyrase) that
combine O2
Heated to drive off O2
Anaerobic Culture Methods
43. Microbial nutrition and growth
Purifying a culture
A pure culture contains only one species or strain
A colony is a population of cells arising from a
single cell or spore or from a group of attached
cells
A colony is often called a colony-forming unit (CFU)
46. 46
Growth of Staphylococcus aureus on Manitol Salt Agar
results in a color change in the media from pink to yellow.
47. Microbial nutrition and growth 47
Laboratory Culture of Microorganisms
Microorganisms can be grown in the
laboratory in culture media containing the
nutrients they require.
Successful cultivation and maintenance of
pure cultures of microorganisms can be
done only if aseptic technique is practiced to
prevent contamination by other
microorganisms.
48. Microbial nutrition and growth 48
Microbial growth
Microbes grow via binary fission, resulting in exponential
increases in numbers
The number of cell arising from a single cell is 2n after n
generations
Generation time is the time it takes for a single cell to grow
and divide
51. Microbial nutrition and growth 51
Growth curve
During lag phase, cells are recovering from a period of no
growth and are making macromolecules in preparation for
growth
During log phase cultures are growing maximally
Stationary phase occurs when nutrients are depleted and
wastes accumulate (Growth rate = death rate)
During death phase death rate is greater than growth rate
52. Microbial nutrition and growth 52
Methods used to measure microbial growth
Count colonies on plate or filter (counts live
cells)
Microscopic counts
Flow cytometry (FACS)
Turbitity
53. Microbial nutrition and growth 53
Viable counts
Each colony on plate or filter arises from single live cell
Only counting live cells
58. Microbial nutrition and growth 58
Microscopic counts
Need a microscope, special slides, high power
objective lens
Typically only counting total microbe numbers, but
differential counts can also be done
59. Microbial nutrition and growth 59
Turbitity
Cells act like large particles
that scatter visible light
A spectrophotometer sends a
beam of visible light through
a culture and measures how
much light is scattered
Scales read in either
absorbance or %
transmission
Measures both live and dead
cells
60. Microbial nutrition and growth 60
Inoculation
Sample is placed on sterile medium providing
microbes with the appropriate nutrients to sustain
growth.
Selection of the proper medium and sterility of all
tools and media is important.
Some microbes may require a live organism or living
tissue as the inoculation medium.
61. Microbial nutrition and growth 61
Incubation
An incubator can be used to adjust the proper growth
conditions of a sample.
Need to adjust for optimum temperature and gas
content.
Incubation produces a culture – the visible growth of
the microbe on or in the media
62. Microbial nutrition and growth 62
Isolation
The end result of inoculation and incubation is isolation.
On solid media we may see separate colonies, and in
broth growth may be indicated by turbidity.
Sub-culturing for further isolation may be required.
63. Microbial nutrition and growth 63
Inspection
Macroscopically observe cultures to note color,
texture, size of colonies, etc.
Microscopically observe stained slides of the culture
to assess cell shape, size, and motility.
64. Microbial nutrition and growth 64
Identification
Utilize biochemical tests to differentiate the microbe
from similar species and to determine metabolic
activities specific to the microbe.