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Introduction to Microbiology, History & scope ,[object Object],- In the broadest sense,  microbiology  is the study of all organisms that are invisible to the naked eye-that is the study of  microorganisms . - Its subjects are viruses, bacteria, many algae and fungi, and protozoa. - The importance of microbiology and microorganisms can not be overemphasized. - Microorganisms are necessary for the production of bread, cheese, beer, antibiotics, vaccines, vitamins, enzymes, etc. - Modern  biotechnology  rests upon a microbiological foundation. ,[object Object],- Microorganisms are everywhere; almost every natural surface is colonized by microbes, from body to ocean. Some microorganisms can live hot springs, and others in frozen sea ice.  - Most microorganisms are harmless to humans; You swallow millions of microbes every day with no ill effects. In fact, we are dependent on microbes to help us digest our food. - Microbes also keep the biosphere running by carrying out essential functions such as decomposition of dead animals and plants. They make possible the cycles of carbon, oxygen, nitrogen and sulfur that take place in terrestrial and aquatic systems. - Microorganisms have also harmed humans and disrupted society over the millennia.
- They sometimes cause diseases in man, animals and plants. They are involved in food spoilage. - Infectious diseases have played major roles in shaping human history (decline of Roman Empire & conquest of the New World. - The "Great Plague", reduced population of western Europe by 25%. - Smallpox and other infectious diseases introduced by European explorers to the Americas in 1500's were responsible for decimating Native American populations. - Until late 1800's, no one had proved that infectious diseases were caused by specific microbes. ,[object Object],- Invisible creatures were thought to exist long before they were observed. -  Antony van Leewenhoek  (1632 – 1723) who invented the first microscope (50 – 300x), was the first to accurately observe and describe microorganisms.
[object Object],- From earliest times, people believed that Living organisms could developed from nonliving or decomposing matter. - The SGT was challenged by Redi, Needham, Spallanzani -  Louis Pasteur  (1822-1895) settled the conflict once for all; heated the necks of flasks and drew them out . ,[object Object],-  Bassi  – showed that silkworm disease was caused by a fungus. -  Berkeley  and  Pasteur  showed that Microorganisms caused disease. -  Joseph Lister  – developed system for sterile surgery -  Robert Koch  (1843 – 1910)  established the relationship between  Bacillus anthracis  and anthrax; also isolated the bacillus that causes tuberculosis.  -  Charles Chamberland  (1851-1908) discovered viruses and their role in disease.
[object Object],- Microorganism must be present in every case of the disease but absent from healthy individuals. - The suspected microorganism must be isolated and grown in pure cultures. - The disease must result when the isolated microorganism is inoculated into a healthy host. - The same microorganism must be isolated from the disease host. ,[object Object],- During Koch’s studies, it became necessary to isolate suspected bacterial pathogens. - He cultured bacteria on the sterile surfaces of cut, boiled potatoes    Not satisfactory. - Regular liquid medium solidified by adding gelatin    gelatin melted @ T>28 °C. - Fannie Eilshemius suggested use of agar; 100 °C to melt, 50 °C to solidify. - Richard Petri developed petri dish, a container for solid culture media. ,[object Object],- Developed  vaccines  for Chickenpox, anthrax, rabies   - Demonstrated that all fermentations were due to the activities of specific yeasts and bacteria.
- Discovered that  fermentative microorganisms  were  anaerobic  and could live only in absence of oxygen. - Developed  Pasteurization   to preserve wine during storage. Important: Foods ,[object Object],-  Winogradsky  made many contributions to soil microbiology; discovered that soil bacteria could oxidize Fe, S and ammonia to obtain energy. - Isolated Anaerobic nitrogen-fixing bacteria; studied the decomposition of cellulose. - Together with  Beijerink , developed the  enrichment-culture  technique and the use of  selective media . - Early 40’s,  Microbiology  established closer relationship with  Genetics  and  Biochemistry ; microorganisms are extremely useful experimental subjects. - e.g. Study of relationship between genes and enzymes; evidence that DNA is the genetic material;  - Recently,  Microbiology  been a major contributor to the rise of  Molecular Biology.   - Studies on Genetic code; mechanisms of DNA, RNA, and Protein synthesis; regulation of gene expression; control of enzyme activity. - Development of  Recombinant DNA Technology  and  Genetic Engineering .
[object Object],-  Procaryotes:  relative simple morphology and lack true membrane delimited nucleus -  Eucaryotes:  morphologically complex with a true membrane  enclosed nucleus
- Organisms divided into 5  Kingdoms: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],- Many microbiologists are primarily interested in the biology of microorganisms, while others focus on specific groups;  - Microbiology has an impact on medicine, agriculture, food science, ecology, genetics, biochemistry, immunology, and many other fields. - Virologists - viruses - Bacteriologists - bacteria - Phycologists – algae - Mycologist -fungi
- Protozoologists – protozoa -  Medical Microbiology:  deals with diseases of humans and animals; identify and plan measures to eliminate agents causing infectious diseases. -  Immunology:  study of the immune system that protects the body from pathogens. -  Agricultural Microbiology:  impact of microorganisms on agriculture; combat plant diseases that attack important food crops. ,[object Object],-  Industrial Microbiology:  using microorganisms to make products such as antibiotics, vaccines, steroids, alcohols & other solvents, vitamins, amino acids, enzymes, etc. -  Genetic Engineering:  Engineered microorganisms used to make hormones, antibiotics, vaccines and other products.   -  Since  viruses  are  acellular  and possess both living and nonliving characteristics, they are considered neither prokaryotic nor eukaryotic.

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