The mesoderm generates organs between the ectoderm and endoderm. It is divided into four regions - chordamesoderm, paraxial mesoderm, intermediate mesoderm, and lateral plate mesoderm. The lateral plate mesoderm splits into somatic and splanchnic layers, forming the body cavity. The heart develops from cardiogenic mesoderm along the BMP signaling pathway induced by endoderm, while the notochord and neural tube provide inhibitory signals. By 29 hours of incubation, the heart primordia have fused to form a single tube.

1. Mesoderm

2. • The mesoderm generates all the organs
between the ectodermal wall and endodermal
tissues.
• The trunk mesoderm of a neurula stage embryo
is divided into 4 regions
1. Chordamesoderm: This forms notochord. Its
main function is to establish anterior posterior
axis.
2. Paraxial mesoderm: Flanking on both the sides
of notochord. Also called somitic mesoderm.
The cells from this region will form somites.
3. Intermediate mesoderm: forms urinogenital
system.
4. Lateral plate mesoderm: forms heart, blood
vessels and blood cells, lining of the body
cavities, extraembryonic membranes.

5. Lateral Plate Mesoderm
• Each plate splits horizontally into two layers.
• The dorsal layer is somatic mesoderm. It underlies
the ectoderm. Together with ectoderm it forms
somatopleure.
• The ventral layer is the splanchnic mesoderm. It
overlies the endoderm. Together with the
endoderm it forms splanchnopleure.
• The space between these two layers becomes the
body cavity coelom. Coelom stretches from neck
region to the posterior of the body.

6. Development of the heart
• The circulatory system provides nourishment to the
developing vertebrate embryo.
• Circulatory system is the first functional unit in the
developing vertebrate embryo. Heart is the first
functional organ.
• Vertebrate heart arises from two regions of
splanchnic mesoderm-one on each side of the
body.
• The presumptive heart cells originate in the early
primitive streak, just posterior to Hensen’s node.
These cells migrate through the streak and form
two groups of mesodermal cells called cardiogenic
mesoderm.

7. Enoderm adjacent
to heart
If anterior endoderm
removed- no heart formation
BMP pathway
BMP from endoderm
Promote heart and
blood vessels
FGF pathway
BMPs induce Fgf8 synthesis
Fgf8 critical for expression of
Cardiac proteins.

8. • Inhibitory signals: given by notochord
• Notochord secretes Noggin and Chordin
blocking BMP pathway.
• Wnt proteins from neural tube Wnt3a and
Wnt8 inhibit heart formation but promote
blood formation.
• Anterior endoderm produces Wnt inhibitors
such as cerebrus, Dickkopf and crescent.
These prevent binding of Wnt to its
receptor.
• This is how cardiac precursor cells are
specified.

9. • The first definite indication of heart appears at 25 hours
of incubation
• The inner mesoderm of slpanchnopleure just behind the
anterior intestinal portal becomes thick and bends
inwards. The cells proliferate and give out loose cells.
These cells soon form the endocardial primordia of the
inner lining of endothelium.These cells form thick walls
called epimyocardial primordia.

10. • At 27 hours of incubation posterior margins of the
anterior intestinal portal approximate in the mid ventral
line. Gut wall tend to close on ventral side.
• The heart primordia approach each other. By 28 hours
they come close together and by 29 hours they fuse and
form single tube.

11. • A jelly like substance is secreted between outer
epimyocardium and inner endocardium. This substance
is cardiac jelly. It holds both the layers together and
forms a medium through which the cells migrate and
form well organized wall of the heart.
• The fusion forms heart as a short double walled straight
tube held in place by the dorsal and ventral
mesocardium. Vental mesocardium disappears. Later
on dorsal mesocardium also gives way.

12. • At the beginning heart tube is 0.5mm in
length. It becomes elongate and divides
anteriorly into two ventral aortic arches.
• Posteriorly it continues with two
omphalomesenteric veins. Thus now
connected at both ends heart now has
limited space to accommodate growing
length. Therefore now it bends.