The document is a user manual for the Quick-RayTM tissue arraying system. It provides important safety notes about handling biological hazards and following instructions to avoid injury. The manual describes biosafety levels and criteria for working with infectious materials. It explains that tissue microarray technology involves extracting small tissue cores from donor blocks and embedding them in a recipient block, allowing up to 1,000 tissue samples to be analyzed on a single slide. Contact information is provided for the manufacturer, Unitma Co., Ltd.
This document discusses biosafety levels and containment practices for handling biohazardous agents in laboratories. It describes three biosafety levels (BSL1-3) with increasing safety requirements according to the hazard level of the agents. BSL1 requires standard microbiological practices. BSL2 requires controlled access, biological safety cabinets for aerosol-generating procedures, personal protective equipment, and waste decontamination. BSL3 involves handling agents of high risk and requires additional containment such as physical separation of the laboratory, controlled access, required personal protective equipment, decontamination of all waste and vacuum lines, and use of biological safety cabinets for all procedures involving biohazardous materials.
Biosafety refers to ensuring safety when working with biological organisms. This document discusses biosafety concepts and issues including containment levels, biosafety cabinets, and risk assessment. The four biosafety levels range from level 1 posing minimal risk to level 4 posing high individual risk without vaccines or treatments. Biosafety cabinets are used to protect workers and the environment, with class I protecting environment, class II protecting samples and environment, and class III providing maximum protection in BSL-4 labs. Risk assessment considers an organism's pathogenicity, virulence, proliferation ability, and transmission route. Guidelines for recombinant DNA research emphasize risk-based containment and avoiding unnecessary regulation.
The document outlines India's biosafety guidelines for genetically engineered organisms (GEOs). It discusses the various committees that implement the guidelines: the Institutional Biosafety Committee regulates research, the Review Committee on Genetic Manipulation reviews high-risk research, and the Genetic Engineering Approval Committee approves large-scale use of GEOs. The guidelines establish containment levels and review processes to minimize risks from GEOs and ensure public health and environmental safety.
The document discusses biosafety concepts and practices. It begins by defining biosafety as safety from exposure to infectious agents. It then discusses biosafety issues in various disciplines like agriculture, medicine, and chemistry. The rest of the document outlines biosafety concepts, levels, and practices based on guidance from the Biosafety in Microbiological and Biomedical Laboratories (BMBL) including standard microbiological practices, safety equipment, and facility design requirements for different biosafety levels from BSL-1 to BSL-4. It also discusses risk assessment and containment practices for working with various biological hazards.
This document summarizes the key safety rules and procedures for a microbiology laboratory. It outlines 15 important lab safety rules including wearing protective clothing, avoiding food or drink, and reporting any accidents. Important safety equipment like safety showers, fire blankets, and biological safety cabinets are also described. Finally, the four biological safety levels are explained based on the types of microbes associated with each level and their corresponding safety requirements.
This document outlines basic laboratory safety procedures for a medical technology laboratory course. It covers standard operating procedures for personal protective equipment, safe handling of biological and hazardous materials, chemical and gas safety, radiation safety, fire safety, and electrical safety. Key points emphasized include wearing proper PPE like lab coats and gloves, adding acids to water, safe sharps disposal, labeling hazardous materials, separating oxidizing and flammable gases, and knowing emergency procedures for fires, spills, and accidents.
This document discusses biosafety levels and guidelines for working with infectious agents in microbiology laboratories. It begins by explaining the importance of biosafety and outlining prohibited activities. It then describes the four biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for non-dangerous agents, and level 4 requiring the strictest methods for dangerous agents. Each biosafety level is explained in terms of the types of infectious agents handled, standard practices, protective equipment and barriers required to handle those agents safely.
This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.
This document discusses biosafety levels and containment practices for handling biohazardous agents in laboratories. It describes three biosafety levels (BSL1-3) with increasing safety requirements according to the hazard level of the agents. BSL1 requires standard microbiological practices. BSL2 requires controlled access, biological safety cabinets for aerosol-generating procedures, personal protective equipment, and waste decontamination. BSL3 involves handling agents of high risk and requires additional containment such as physical separation of the laboratory, controlled access, required personal protective equipment, decontamination of all waste and vacuum lines, and use of biological safety cabinets for all procedures involving biohazardous materials.
Biosafety refers to ensuring safety when working with biological organisms. This document discusses biosafety concepts and issues including containment levels, biosafety cabinets, and risk assessment. The four biosafety levels range from level 1 posing minimal risk to level 4 posing high individual risk without vaccines or treatments. Biosafety cabinets are used to protect workers and the environment, with class I protecting environment, class II protecting samples and environment, and class III providing maximum protection in BSL-4 labs. Risk assessment considers an organism's pathogenicity, virulence, proliferation ability, and transmission route. Guidelines for recombinant DNA research emphasize risk-based containment and avoiding unnecessary regulation.
The document outlines India's biosafety guidelines for genetically engineered organisms (GEOs). It discusses the various committees that implement the guidelines: the Institutional Biosafety Committee regulates research, the Review Committee on Genetic Manipulation reviews high-risk research, and the Genetic Engineering Approval Committee approves large-scale use of GEOs. The guidelines establish containment levels and review processes to minimize risks from GEOs and ensure public health and environmental safety.
The document discusses biosafety concepts and practices. It begins by defining biosafety as safety from exposure to infectious agents. It then discusses biosafety issues in various disciplines like agriculture, medicine, and chemistry. The rest of the document outlines biosafety concepts, levels, and practices based on guidance from the Biosafety in Microbiological and Biomedical Laboratories (BMBL) including standard microbiological practices, safety equipment, and facility design requirements for different biosafety levels from BSL-1 to BSL-4. It also discusses risk assessment and containment practices for working with various biological hazards.
This document summarizes the key safety rules and procedures for a microbiology laboratory. It outlines 15 important lab safety rules including wearing protective clothing, avoiding food or drink, and reporting any accidents. Important safety equipment like safety showers, fire blankets, and biological safety cabinets are also described. Finally, the four biological safety levels are explained based on the types of microbes associated with each level and their corresponding safety requirements.
This document outlines basic laboratory safety procedures for a medical technology laboratory course. It covers standard operating procedures for personal protective equipment, safe handling of biological and hazardous materials, chemical and gas safety, radiation safety, fire safety, and electrical safety. Key points emphasized include wearing proper PPE like lab coats and gloves, adding acids to water, safe sharps disposal, labeling hazardous materials, separating oxidizing and flammable gases, and knowing emergency procedures for fires, spills, and accidents.
This document discusses biosafety levels and guidelines for working with infectious agents in microbiology laboratories. It begins by explaining the importance of biosafety and outlining prohibited activities. It then describes the four biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for non-dangerous agents, and level 4 requiring the strictest methods for dangerous agents. Each biosafety level is explained in terms of the types of infectious agents handled, standard practices, protective equipment and barriers required to handle those agents safely.
This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.
The document discusses recommended biosafety levels for infectious agents and infected animals. It provides details on four biosafety levels (BSL), from BSL-1 which is for agents not known to consistently cause disease, to BSL-4 which is for dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease. Each BSL level outlines the types of agents, laboratory practices, safety equipment as primary barriers, and facility requirements as secondary barriers needed to contain the infectious agents.
The r-DNA Biosafety Guidelines of India classify research activities into three categories based on risk. Category I requires only intimation, Category II requires prior permission, and Category III requires review and approval before starting. The guidelines aim to regulate research safely and minimize accidental release of GMOs. Implementation occurs through four committees - RDAC provides regulatory oversight, IBSC monitors research, RCGM reviews risks and permissions, and GEAC approves large-scale use and environmental release. The guidelines establish containment levels and procedures to safely conduct r-DNA research and ensure biosafety.
The document discusses various plant pathogens that can pose risks to humans working in research facilities if not handled carefully. It outlines different levels of containment from basic to PPC-3 based on the risk of plant pests escaping into the environment. Certain bacteria, fungi, and viruses isolated from plants have been shown to cause infectious diseases in humans and should be studied using higher containment levels.
This presentation clearly explains you about the Biosafety Level 2.
Its includes the requirements, practices, organisms, equipment and uses of Biosafety level 2.
In the U.S., the Centers for Disease Control and Prevention (CDC) and World Health Organization (WHO) specify four biosafety levels. Each level is a series of biosafety requirements for labs conducting research on biological agents.⠀⠀⠀
⠀⠀⠀⠀
We’ve put together this helpful carousel to understand each level, the agents researched at each, and the proper protocol and design incorporated to make sure each biosafety lab level is safe, secure, and best equipped to handle the research they conduct. ⠀⠀⠀
⠀⠀⠀
For example, in relation to current events, the Coronavirus is studied at a level 3 biosafety lab (BSL-3) and less at-risk agents like E.coli are studied in a level 1 biosafety lab (BSL-1).⠀⠀⠀
⠀⠀⠀
Follow OnePointe Solutions for more scientific related topics.
The document discusses biosafety guidelines for microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 having the fewest precautions for the least dangerous agents, and level 4 having the strictest methods for working with highly dangerous agents. The key precautions at each level include engineering controls, personal protective equipment, facility design, and standard practices to prevent exposure to infectious agents. Adhering to biosafety levels and guidelines is important to protect laboratory workers from acquiring infections.
The document summarizes Bangladesh's biosafety regulatory system and its evolution over time. Some key points:
- Bangladesh started developing its biosafety framework in the 1990s and ratified the Cartagena Protocol on Biosafety in 2004.
- The biosafety regulatory system includes biosafety guidelines, a national biosafety framework, and biosafety rules gazetted in 2012.
- Various committees provide governance, including the National Committee on Biosafety, Biosafety Core Committee, Institutional Biosafety Committees, and Field Level Biosafety Committees
04 control of spills and mechanism of implementation of biosafety guidelinesIndranil Bhattacharjee
1. The document outlines procedures for controlling spills of biological materials in laboratories, including spills in biological safety cabinets, open laboratories, centrifuges, and on persons. It describes wearing protective equipment, warning others, and using appropriate disinfectants.
2. It then describes the mechanism for implementing biosafety guidelines in India, including committees that provide oversight of recombinant DNA research. The key committees are the Recombinant DNA Advisory Committee, Institutional Biosafety Committees, and the Review Committee on Genetic Manipulation.
3. The committees provide guidance on biosafety, review research proposals, oversee safety training, and ensure containment facilities and procedures are followed to regulate genetic engineering activities and protect
This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.
This document discusses laboratory biosafety levels and the transfer of biological samples. It begins by outlining the objectives and presentation outline. It then introduces biosafety practices and discusses the four biosafety levels - BSL-1 to BSL-4 - in increasing order of risk. Each level is associated with different safety practices and containment facilities depending on the risk of pathogens handled. The document also discusses regulations for the safe transfer of biological samples between facilities, including packaging, labeling and documentation requirements. Material transfer agreements are identified as important for governing the transfer and use of biological materials.
The document outlines a training program called PRO TECH for pest control technicians in India. The objectives are to raise standards in the industry through education and training, instill confidence in technicians, and promote eco-friendly and safe working practices. The 36-hour course would train both existing technicians and unemployed youth, covering topics like pest biology, management techniques, safety protocols, and customer service skills. Assessment includes written tests, and trainees would receive a certificate upon completion. The goal is to formalize training and improve services for the estimated 25,000 operators in India.
This document discusses biosafety principles for microbiology and biomedical laboratories. It defines biosafety and outlines key concepts like biohazards, biosafety levels, and the biohazard symbol. Biosafety aims to minimize health and environmental risks from hazardous biological materials through administrative controls, safety equipment, and facility design tailored to the risks involved. The document also notes emerging issues at the intersection of biosafety and biotechnology like genetically modified organisms, biosecurity, and bioethics.
A persistent,retentive,durable,Non-toxic,non-migrating broad spectrum antimicrobial for all porous and non porous surfaces.
Applied on Textiles,metal,plastics,ceramics,rubber,leather,glass,porcelain,grouts,sand,carbon black,cement surfaces,paints,coatings etc
rDNA Technology-Biosafety Regulations and GuidelinesRahul Kumar
This document discusses guidelines for biosafety regulations and recombinant DNA (rDNA) technology in India. It outlines the various committees established to develop policies and safety guidelines for rDNA research, oversee projects, and approve large-scale activities. The guidelines cover containment levels, classification of pathogens, research oversight, environmental release of engineered organisms, import/export, and quality control of products developed through rDNA technology.
Understanding How Bioburden and Sterilization Affect Medical DevicesPacific BioLabs
1) Bioburden refers to microorganisms present on medical devices and must be monitored and controlled as high levels can compromise sterilization validation.
2) Sterilization methods like gamma, e-beam, and ethylene oxide are effective when bioburden levels are properly determined and accounted for in the sterilization dose.
3) Maintaining low and consistent bioburden through environmental monitoring, personnel training, cleaning processes, and device design is essential for ensuring sterilized medical devices.
Laboratory hazards, safety and contaminationNadjo Deen
This document discusses laboratory hazards, safety, and contamination. It identifies three main types of laboratory hazards: chemical, biological, and physical. Examples of each hazard are provided. The document also discusses sterilization methods like heat, chemicals, filtration, and radiation that are used to eliminate microorganisms. Specific sterilization techniques like autoclaving, flaming, and UV light are explained. The document concludes with recommendations for general laboratory safety practices.
دورة السلامة الكيمياوية والامن البايولوجي في المختبراتSuraLateefSalman
Laboratory safety measures aim to protect against accidents. This includes safety training, enforcing safety policies, reviewing experimental designs, using personal protective equipment, and employing the buddy system for risky operations. Proper storage and handling of chemicals is important to minimize dangerous interactions. Chemical Safety Data Sheets and compatibility information must be consulted to safely store chemicals. Personal protective equipment like closed-toe shoes, long pants, and safety glasses are minimum requirements in labs. Spills of acids, bases, and biological materials require appropriate neutralization and clean-up procedures.
Maria Santos from Essencius, Denmark, is doing a presentation on the development of Social Media in corporate branding at the first JOB2SEA seminar in Elsinore 20. nov. 2012.
The proposal includes 13 items to be provided as part of the project: 1) an attractive website design, 2) a dynamic CMS website, 3) eyelash extensions, 4) a student information system, 5) an e-learning portal integrated with the main site, 6) business email hosting through Google, 7) business intranet applications through Google, 8) a career discussion bulletin board, 9) web traffic monitoring through Google Analytics, 10) an online payment gateway, 11) an online counselor desk, 12) a customer relationship management system, and 13) an online marketing program through Google AdWords.
The document outlines a trip to several US cities including Miami, New York, Chicago, and Orlando. It lists attractions, restaurants, and activities in each location. It also provides a budget breakdown for the trip, showing $4000 was budgeted and $3894.57 was spent.
The document discusses recommended biosafety levels for infectious agents and infected animals. It provides details on four biosafety levels (BSL), from BSL-1 which is for agents not known to consistently cause disease, to BSL-4 which is for dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease. Each BSL level outlines the types of agents, laboratory practices, safety equipment as primary barriers, and facility requirements as secondary barriers needed to contain the infectious agents.
The r-DNA Biosafety Guidelines of India classify research activities into three categories based on risk. Category I requires only intimation, Category II requires prior permission, and Category III requires review and approval before starting. The guidelines aim to regulate research safely and minimize accidental release of GMOs. Implementation occurs through four committees - RDAC provides regulatory oversight, IBSC monitors research, RCGM reviews risks and permissions, and GEAC approves large-scale use and environmental release. The guidelines establish containment levels and procedures to safely conduct r-DNA research and ensure biosafety.
The document discusses various plant pathogens that can pose risks to humans working in research facilities if not handled carefully. It outlines different levels of containment from basic to PPC-3 based on the risk of plant pests escaping into the environment. Certain bacteria, fungi, and viruses isolated from plants have been shown to cause infectious diseases in humans and should be studied using higher containment levels.
This presentation clearly explains you about the Biosafety Level 2.
Its includes the requirements, practices, organisms, equipment and uses of Biosafety level 2.
In the U.S., the Centers for Disease Control and Prevention (CDC) and World Health Organization (WHO) specify four biosafety levels. Each level is a series of biosafety requirements for labs conducting research on biological agents.⠀⠀⠀
⠀⠀⠀⠀
We’ve put together this helpful carousel to understand each level, the agents researched at each, and the proper protocol and design incorporated to make sure each biosafety lab level is safe, secure, and best equipped to handle the research they conduct. ⠀⠀⠀
⠀⠀⠀
For example, in relation to current events, the Coronavirus is studied at a level 3 biosafety lab (BSL-3) and less at-risk agents like E.coli are studied in a level 1 biosafety lab (BSL-1).⠀⠀⠀
⠀⠀⠀
Follow OnePointe Solutions for more scientific related topics.
The document discusses biosafety guidelines for microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 having the fewest precautions for the least dangerous agents, and level 4 having the strictest methods for working with highly dangerous agents. The key precautions at each level include engineering controls, personal protective equipment, facility design, and standard practices to prevent exposure to infectious agents. Adhering to biosafety levels and guidelines is important to protect laboratory workers from acquiring infections.
The document summarizes Bangladesh's biosafety regulatory system and its evolution over time. Some key points:
- Bangladesh started developing its biosafety framework in the 1990s and ratified the Cartagena Protocol on Biosafety in 2004.
- The biosafety regulatory system includes biosafety guidelines, a national biosafety framework, and biosafety rules gazetted in 2012.
- Various committees provide governance, including the National Committee on Biosafety, Biosafety Core Committee, Institutional Biosafety Committees, and Field Level Biosafety Committees
04 control of spills and mechanism of implementation of biosafety guidelinesIndranil Bhattacharjee
1. The document outlines procedures for controlling spills of biological materials in laboratories, including spills in biological safety cabinets, open laboratories, centrifuges, and on persons. It describes wearing protective equipment, warning others, and using appropriate disinfectants.
2. It then describes the mechanism for implementing biosafety guidelines in India, including committees that provide oversight of recombinant DNA research. The key committees are the Recombinant DNA Advisory Committee, Institutional Biosafety Committees, and the Review Committee on Genetic Manipulation.
3. The committees provide guidance on biosafety, review research proposals, oversee safety training, and ensure containment facilities and procedures are followed to regulate genetic engineering activities and protect
This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.
This document discusses laboratory biosafety levels and the transfer of biological samples. It begins by outlining the objectives and presentation outline. It then introduces biosafety practices and discusses the four biosafety levels - BSL-1 to BSL-4 - in increasing order of risk. Each level is associated with different safety practices and containment facilities depending on the risk of pathogens handled. The document also discusses regulations for the safe transfer of biological samples between facilities, including packaging, labeling and documentation requirements. Material transfer agreements are identified as important for governing the transfer and use of biological materials.
The document outlines a training program called PRO TECH for pest control technicians in India. The objectives are to raise standards in the industry through education and training, instill confidence in technicians, and promote eco-friendly and safe working practices. The 36-hour course would train both existing technicians and unemployed youth, covering topics like pest biology, management techniques, safety protocols, and customer service skills. Assessment includes written tests, and trainees would receive a certificate upon completion. The goal is to formalize training and improve services for the estimated 25,000 operators in India.
This document discusses biosafety principles for microbiology and biomedical laboratories. It defines biosafety and outlines key concepts like biohazards, biosafety levels, and the biohazard symbol. Biosafety aims to minimize health and environmental risks from hazardous biological materials through administrative controls, safety equipment, and facility design tailored to the risks involved. The document also notes emerging issues at the intersection of biosafety and biotechnology like genetically modified organisms, biosecurity, and bioethics.
A persistent,retentive,durable,Non-toxic,non-migrating broad spectrum antimicrobial for all porous and non porous surfaces.
Applied on Textiles,metal,plastics,ceramics,rubber,leather,glass,porcelain,grouts,sand,carbon black,cement surfaces,paints,coatings etc
rDNA Technology-Biosafety Regulations and GuidelinesRahul Kumar
This document discusses guidelines for biosafety regulations and recombinant DNA (rDNA) technology in India. It outlines the various committees established to develop policies and safety guidelines for rDNA research, oversee projects, and approve large-scale activities. The guidelines cover containment levels, classification of pathogens, research oversight, environmental release of engineered organisms, import/export, and quality control of products developed through rDNA technology.
Understanding How Bioburden and Sterilization Affect Medical DevicesPacific BioLabs
1) Bioburden refers to microorganisms present on medical devices and must be monitored and controlled as high levels can compromise sterilization validation.
2) Sterilization methods like gamma, e-beam, and ethylene oxide are effective when bioburden levels are properly determined and accounted for in the sterilization dose.
3) Maintaining low and consistent bioburden through environmental monitoring, personnel training, cleaning processes, and device design is essential for ensuring sterilized medical devices.
Laboratory hazards, safety and contaminationNadjo Deen
This document discusses laboratory hazards, safety, and contamination. It identifies three main types of laboratory hazards: chemical, biological, and physical. Examples of each hazard are provided. The document also discusses sterilization methods like heat, chemicals, filtration, and radiation that are used to eliminate microorganisms. Specific sterilization techniques like autoclaving, flaming, and UV light are explained. The document concludes with recommendations for general laboratory safety practices.
دورة السلامة الكيمياوية والامن البايولوجي في المختبراتSuraLateefSalman
Laboratory safety measures aim to protect against accidents. This includes safety training, enforcing safety policies, reviewing experimental designs, using personal protective equipment, and employing the buddy system for risky operations. Proper storage and handling of chemicals is important to minimize dangerous interactions. Chemical Safety Data Sheets and compatibility information must be consulted to safely store chemicals. Personal protective equipment like closed-toe shoes, long pants, and safety glasses are minimum requirements in labs. Spills of acids, bases, and biological materials require appropriate neutralization and clean-up procedures.
Maria Santos from Essencius, Denmark, is doing a presentation on the development of Social Media in corporate branding at the first JOB2SEA seminar in Elsinore 20. nov. 2012.
The proposal includes 13 items to be provided as part of the project: 1) an attractive website design, 2) a dynamic CMS website, 3) eyelash extensions, 4) a student information system, 5) an e-learning portal integrated with the main site, 6) business email hosting through Google, 7) business intranet applications through Google, 8) a career discussion bulletin board, 9) web traffic monitoring through Google Analytics, 10) an online payment gateway, 11) an online counselor desk, 12) a customer relationship management system, and 13) an online marketing program through Google AdWords.
The document outlines a trip to several US cities including Miami, New York, Chicago, and Orlando. It lists attractions, restaurants, and activities in each location. It also provides a budget breakdown for the trip, showing $4000 was budgeted and $3894.57 was spent.
JOB2SEA is a maritime job board started in 2010 by Jakob le Fevre, a master mariner with 40 years of experience in the maritime industry. It connects maritime companies and jobseekers by allowing companies to post jobs and candidates to upload CVs. The site has experienced strong growth, receiving over 50,000 visits per month. It also partners with LinkedIn to further expand its reach. JOB2SEA provides an affordable alternative for companies to traditional online and print job advertising methods.
Dokumen ini membahas penentuan kualitas daging pascapanen dan penyimpanan suhu dingin. Kualitas daging masih baik selama enam jam penyimpanan awal dan satu minggu penyimpanan beku, namun menurun setelah dua minggu penyimpanan beku. Daging segar memiliki warna merah, tekstur kenyal, dan jumlah mikroba rendah.
Dokumen ini membahas penelitian tentang penanganan pascapanen buah pisang dengan tiga varietas (ambon, janten, muli) dan tiga perlakuan (kontrol, memar, luka). Penelitian mengamati jenis jamur dan gejala busuk pada setiap varietas dan perlakuan selama enam hari. Hasilnya menunjukkan pisang ambon lebih rentan terhadap busuk dibanding jenis lain karena diinfeksi Lasiodiplodia theobromae dan T.
Baromètre synthétique des filières d'énergies renouvelables réalisé par le consortium EurObserver. Disponible sur → [http://www.eurobserv-er.org/14th-annual-overview-barometer/]
7 conseils pour améliorer son référencement naturel (SEO)Editoile
Comment faire remonter son site internet dans les résultats des moteurs de recherche ? Quelles sont les bonnes pratiques actuelle du SEO (Search Engine Optimization) ? Par quoi commencer pour améliorer le référencement naturel ou organique de ses contenus web ?
On y parle Google, Panda, Pingouin, Colibri, expressions-clés, longue traîne, cocon sémantique, requêtes conversationnelles, content marketing, rédacteurs web, etc.
Cette présentation a été diffusée par Gabrielle Denis, directrice de l'agence de contenu web Editoile à Bordeaux, lors d'une matinée spéciale référencement naturel et payant organisée avec Generaleads.
Lors d'un projet pédagogique à HETIC, nos étudiants en 3e année ont dû imaginer et concevoir un nouveau jeu mobile pour la société Oasis. http://hetic.net
Voici le sujet donné : "Déjà connu pour ses campagnes au ton décalé, Oasis utilise des supports de communication classiques, mais s’appuie également sur les réseaux sociaux et le mobile (« La chuuute sur mer » et « La chuuute »).
Décidé à aller encore plus loin, Oasis souhaite utiliser d’une part, la prédiction des Mayas comme axe principal de sa prochaine campagne et d’autre part le support mobile, le tout en gardant son côté fun et fruité."
Refonte digitale du site du Château de Versailles dans le cadre d'un appel d'offres fictif à HETIC.
Plus d'informations sur : http://www.hetic.net/actualites/projets/redesign-du-chateau-de-versailles
Equipe dirigée par : Louis CHARACHON
Oscar DELOIZY
Maxime GUIONEAU
Centis MENANT
Maxime ROBIN
The document discusses containment methods for safely handling biohazardous agents in laboratories. Primary containment refers to protecting laboratory personnel through good microbiological techniques and safety equipment like biological safety cabinets. Secondary containment protects the external environment through facility design and operational practices. The purpose of containment is to reduce exposure risks and prevent escape of potentially biohazardous agents.
The document discusses containment methods for safely handling biohazardous agents in laboratories. Primary containment involves good microbiological techniques and safety equipment like biological safety cabinets to protect personnel and the immediate lab environment. Secondary containment uses facility design and practices to protect the external environment. Containment aims to reduce exposure risks for lab workers and prevent agents from escaping outside. The document then describes different biosafety levels based on the hazard level of the agents and appropriate safety measures for each level.
General Laboratory Safety full slideshow.pptxAhnaf maznun
This document provides guidance on laboratory safety policies and procedures. It outlines the roles and responsibilities of management, laboratory supervisors, and individual employees to ensure a safe work environment. General safety rules are established, such as following evacuation plans, using protective equipment properly, and disposing of waste correctly. Hazards in the laboratory including chemical, biological, physical, and electrical dangers are discussed. The importance of safety data sheets, labeling, signage, and personal protective equipment to minimize risks is also explained.
Lecture 1 introduction of biosafety & biosecurityraghdasaad6
This lecture introduces biosafety and biosecurity. Biosafety refers to containment practices to prevent exposure to pathogens, while biosecurity aims to prevent the spread of harmful organisms. Biorisk encompasses both biosafety and biosecurity risks associated with biological materials. Key components of biorisk management include risk assessment, mitigation actions, and performance evaluation to continually improve the system. Effective management systems follow a plan-do-check-act cycle. Components of safety in all labs include safe handling of specimens, chemicals, instruments, and waste as well as fire and electrical safety.
This document provides guidelines for good laboratory practice when conducting experiments. It recommends that students: be familiar with laboratory procedures before attempting them; work carefully to avoid injuries; work with a partner when possible; handle materials carefully; wear proper protective equipment; keep the work area clean and organized; and report any accidents. The guidelines are intended to promote safety in the laboratory.
This document discusses biosafety precautions and laboratory acquired infections. It begins by introducing biosafety and biosafety levels 1 and 2. Biosafety level 1 is for well characterized agents that do not consistently cause disease, while level 2 requires specific training and procedures when working with agents that pose moderate hazards. The document then discusses principles of biosafety like containment and risk assessment. It also provides details on safety equipment, facility design, standard microbiological practices and national biosafety guidelines to prevent laboratory acquired infections.
This document provides definitions and concepts related to biosafety and biosecurity. It defines key terms like biological laboratory, biological agent, biorisk, biorisk assessment, biorisk management, biosafety, biological safety cabinet, biosecurity, and laboratory biosecurity. It discusses establishing a biorisk management system including developing a biorisk management policy, planning for hazard identification and risk assessment, and setting biorisk control objectives. It also outlines important components of safety in the laboratory, including emergency procedures, signage, safety equipment, and basic lab rules. Finally, it discusses universal safety precautions like the use of barriers, hand washing, safe handling of sharps, and decontamination procedures.
The document discusses biosafety levels (BSL) from 1-3. BSLs are determined by factors like risk of infection, nature of work, and agent characteristics. Each level has standardized practices and facilities appropriate for the associated risks. BSL1 applies to low-risk agents and requires basic precautions. BSL2 is for agents associated with human disease, requiring enhanced safety measures. BSL3 is for indigenous agents that can cause serious disease through inhalation and has strict controls, medical surveillance, and restricted access. Higher BSLs build upon practices of lower levels with increasing containment requirements to protect laboratory personnel and the public.
This document discusses biosafety and biocontainment levels. It describes the risks associated with biological research, including hazards from pathogens and laboratory procedures. Pathogens are classified into four risk groups based on infectivity and severity of disease. Containment levels including biological and physical containment help reduce exposure risks. Primary containment involves laboratory practices and equipment while secondary containment involves facility design. Facilities are designated as biosafety level I, II or III depending on the risk group of pathogens handled.
Introduction to medical equipments safety and testingMEHABOOB RAHMAN
Medical equipment can present a variety of hazards including mechanical, electrical, fire-related, and those resulting from improper function or incorrect output. It is important to properly maintain equipment and perform safety testing using calibrated equipment to minimize risks to patients and users. Regular performance testing helps ensure medical devices are functioning as intended.
Biosafety and ethics in Molecular biology laboratoryAyesha Aftab
This document provides guidelines for biosafety in a molecular biology laboratory. It discusses the principles of biosafety including containment, risk assessment, and standard practices. It describes necessary laboratory facilities, equipment, and procedures to prevent exposure to biological hazards. This includes recommendations for laboratory design, standard and special practices, safety equipment, waste disposal, transportation of agents, and more. The overall goal is to establish a safe work environment and protect laboratory workers, the public, and environment from potential biological risks.
This document discusses sterile products and clean room classifications. Sterile products must be free from microorganisms and pyrogens. They include parenterals, ophthalmics, and irrigation fluids. Several factors are important for sterile compounding including clean facilities, trained personnel, and sterilization/stability principles. Clean rooms are classified based on particulate levels, with grades A through D (or classes 100 through 100,000) used in pharmaceutical facilities. Grade A/class 100 areas are needed for high-risk aseptic operations.
This document discusses biosafety and biosafety levels. It defines biosafety as steps taken to protect humans, products, and the environment from biological hazards that may occur from research or commerce involving infectious organisms or genetically modified organisms. The document then describes the four biosafety levels established by the CDC - Biosafety Level 1 requires basic safety precautions; Biosafety Level 2 requires more extensive precautions for work with pathogens that pose moderate hazards; Biosafety Level 3 is for dangerous indigenous or exotic agents that may cause severe disease through inhalation; and Biosafety Level 4 contains the most hazardous pathogens and requires the highest level of containment.
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
This document discusses lab safety rules, standard precautions, patient safety, and staff safety. It outlines rules for proper lab attire including wearing laboratory coats, closed-toe shoes, and covering legs. Standard precautions treat all body fluids as potentially infectious. Biosafety levels 1-4 are described based on the risk of pathogens. Electrical, fire, chemical, biological, and mechanical hazards in labs are addressed. Procedures for needlestick injuries include first aid, reporting, and testing. The goals of patient safety are to prevent harm and errors related to equipment, identity, specimens, falls, delays, and contamination.
The document defines biosafety levels and containment controls for working with biological agents. There are four biosafety levels that provide increasing containment based on the risk group of the agent. BSL-1 is for low risk agents and allows open bench work, while BSL-4 is for dangerous exotic agents and requires the highest level of containment like positive pressure suits. Each level has specific laboratory practices, safety equipment, and facility requirements to safely contain biological agents and protect laboratory workers from exposure.
3. Tissue array using Quick-Ray™
IMPORTANT NOTES
• Explanation of Symbols used
WARNING: WARNING indicates an injury hazard not immediately accessible
as you read this symbol. It calls attention to an operating procedure, practice,
or like that, if no correctly performed or adhered to, could result in personal
injury or death. Do not proceed beyond a WARNING notice until the
indicated conditions are fully understood and met.
BIOHAZARD WARNING: BIOHAZARD WARNING indicates an injury hazard
not immediately accessible as you read this symbol.
Biohazard(Infectious agent): A type of micro-organism, bacteria, mold parasite
or virus which normally causes, or significantly contributes to the cause of,
increased morbidity or mortality of human beings.
• SAFETY NOTES
Read the following safety notes to avoid injury and prevent damage to this
product or any products connected to it.
To avoid personal injury
• Be careful not to pinch your fingers when closing the wooden case.
• Keep the Quick-RayTM and accessories out of the reach of small children.
• Be careful for your fingers or hand not to be scratched by the needle of tip
when assembling or disassembling the tip or the probe, or when cleaning the
tip.
Use the Quick-RayTM and accessories according to the instructions in this manual
• Use the Quick-RayTM and accessories according to the instructions in this
manual. No authorization for the analysis or modification of the Quick-RayTM is
provided.
• Do not use accessories that are not supplied or recommended by UNITMA.
• Contact UNITMA for repairing or service or buying accessories.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
4. Tissue array using Quick-Ray™
Do not use the donor block or a sample with a biological hazard or a chemical hazard.
• Do not use the donor block or a sample with a biological hazard or a chemical
hazard that can impact human health.
Handle biohazards.
• Working with infectious material requires the following precautions.
a. Limit access to areas where experiments with infectionus specimens are in
progress.
b. Clearly label areas where biohazards are in use and designate specific
areas where biohazards are routinely used, using this symbol (black on red
background):
c. Wear lab coat, gloves and safety glasses to prevent contamination from the
infectious specimen, and remove them when leaving the work area.
d. Decontaminate work surfaces once per day and after any spill of viable
specimen.
e. Eating, drinking and applying cosmetics are not permitted in the work area.
f. Wash hands after handling viable specimens before leaving the lab.
g. Transport contaminated materials in a red biohazard bag which has been
placed in a labeled, leak-proof container.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
5. Tissue array using Quick-Ray™
Biohazard Determination.
• Lab Biosafety Level Criteria.
The following guidelines can be used by all laboratory personel.
a. Biosafety Level 1 is appropriate for undergraduate and secondary
educational training and teaching laboratories and/or other facilities in which
work is done with defined and characterized strains of viable microorganisms
not known to cause disease in healthy adult humans.
b. Biosafety Level 2 is applicable to clinical, diagnostic, teaching and other
facilities in which work is done with the broad spectrum of indigenous
moderate-risk agents present in the community and associated with human
disease of varying severity.
c. Biosafety Level 3 is applicable to clinical, diagnostic, teaching, research, or
production facilities in which work is done with indigenous or exotic agents
where the potential for infection by aerosols is real and the disease may have
serious or lethal consequences.
d. Biosafety Level 4 is applicable to work with dangerous and exotic agents
which pose a high individual risk of life-threatening disease.
• Practices and Techniques, Safety Equipment
Biosafety Practices and
Safety Equipment Facilites
Level Techniques
None: primary containment
Standard provided by adherence to
1 microbilogical standard laboratory Basic
practices practices during open bench
operations.
Level 1 practices plus: Partial containment
Laboratory coats; equipment (i.e., Class I or II
decontamination of all Biological Safety Cabinets)
infectious wastes; used to conduct mechanical
2 limited access; manipulative procedures Basic
protective gloves and that have high aerosol
biohazard warning potential that may increase
signs the risk of exposure to
as indicated. personnel.
Level 2 practices plus: Partial containment
special laboratory equipment used for all
3 Containment
clothing; controlled manipulations of infectious
access. material.
No facilities available Maximum
Maximum containment
4 on this campus at this Containment
equipment.
time.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
6. Tissue array using Quick-Ray™
Tissue Microarray ?
"Tissue Microarray (TMA)" is the technology to attach the individual tissue cores in one slide.
The TMA consists of cylindrical paraffin embedded tissues (cores) that are acquired from donor
blocks. "The donor block" is a standard tissue block from surgical pathology, autopsy or research
material. Donor blocks are marked on both slides and tissue blocks to identify morphologically
representative areas of interest. The tissue cores are extracted from the donor block and
inserted into a recipient block. Tissues can be inserted with up to 1000 tissue cores in a recipient
block by using a precise instrument or tool. Thin cross-sections cut from the recipient block (TMA
block) with a microtome are placed onto standard slides for in situ analysis. One TMA block can
generate between 100 and 500 sections.
These TMA technology can attach tissues of several patients or animals on only one slide, and
compare analysis on several genes and protein expression of same tissue, and execute analysis
under same condition. So, it reduces reagent and time, and expenses to 1/60, and increases
research effectiveness. TMA is used widely in recent pathological research analyses, and it is
applicable to most methods used as tissues like Immunohistochemistry, in situ hybridization,
FISH, in situ PCR and so on Tissue.
But the manufacturing of TMA is a critical step in the success of the technology. This creates
tremendous obstacles and impediments for the pathologists to fully utilize the power of TMAs.
Quick-Ray™, the Patented Technologies of UNITMA, overcame these road blocks and
decreased the current time to create Block and Slide in innovative way by providing solutions to
empower the pathologist to easily set up their TMAs with ready-to-go and pre-formed recipient
blocks.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
7. Tissue array using Quick-Ray™
Quick-Ray™ SYSTEM
• Minimized size arrayer comparing with the conventional products
• Portable, easy to carry and to make the array block anytime and anywhere
• Easy to handle. Inexperienced pathologist can handle easily in the lab.
• Simple developing procedure
• Easy to create the various sized block with using disposable bases
• Steel array block and cassette array block are available
• Low cost to purchase
TISSUE MICROARRAY SET
Quick-RayTM • Quick-Ray™
Weight : 140g
Length : 15.5Cm
• Tip & Recipient block
Tip core size
∅ 1mm
∅ 2mm
∅ 3mm
Base Mold ∅ 5mm
Recipient block
• Guide for 1mm recipient block
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
8. Tissue array using Quick-Ray™
CONSUMABLES
• Disposable-base (Recipient block) • Cassette & Base mold
∅ 5mm x 20 well
∅ 3mm x 30 well
∅ 2mm x 60 well
∅ 1mm x 120well
APPLICATION
• Special stain
• Immunohistochemistry
• in situ hybridization
• FISH
• in situ PCR etc.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
9. Tissue array using Quick-Ray™
PROCEDURE
• How to build
1. Place the reference slide and the donor block on
microscope stage for position marking with an oil pen.
2. Extract the marked tissue from the donor block by using the Quick-Ray™ needle.
1) Place the donor block on a horizontal and flat table.
2) Hold the Quick-Ray™ in your hand and tighten your grip.
3) Hold the Quick-Ray™needle perpendicular to the marked
position of the donor block.
4) Insert the Quick-Ray™ needle into the donor block at the
proper depth of 5mm slowly.
(Don’t insert it quickly and too deep to prevent to damage
the donor block and the Quick-Ray™ needle.)
* Quick- Ray™ needle’s depth: 5mm
* Incubate the easily breaking donor block in a heating oven or a chamber at 37~40℃ for 15
~ 20 minutes.
3. Deliver the extracted tissue into the corresponding holes of the recipient block that were pre-
made by UNITMA, with Quick-Ray™ needle.
1) Place the recipient block on a horizontal and flat table.
2) Hold the Quick-Ray™ needle with the extracted tissue
perpendicular to the corresponding holes of the recipient
block.
3) Inject the extracted tissue (core) into the corresponding
holes of the recipient block at the proper depth of 4mm by
pushing the Quick-Ray™ plunger slowly.
4) Adjust the height of all the core with a same settings by
pressing the top of all the core with a flat side of a thing.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
10. Tissue array using Quick-Ray™
4. Put the recipient block into embedding mold with
cutting section faced down and incubate it in oven
at about 70℃ for 30~60 minutes.
(The top side of the recipient block in ‘Step 3’ will
be cutting section.)
5. Take out the recipient block when completely transparent. ⇒ Embedding
Step 1 for Embedding Step 2 for Embedding Step3 for Embedding
6. Solidify the block in cold plate.
7. Cutting (about 4㎛)
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
11. Tissue array using Quick-Ray™
• How to use the guide
1. Put the recipient block into the prop hole at bottom side.
2. Fit the 1mm guide on the prop.
3. Extract the marked tissue from the donor block by using Quick-Ray™ needle.
4. With fixing the prop by hand, insert the Quick-Ray™ needle into the guide hole, and put the
extracted tissue into the recipient block hole by pushing the knob down.
5. Remove the guide and recipient block from the prop.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
12. Tissue array using Quick-Ray™
6. Put the recipient block into embedding mold with cutting section faced down and incubate it in oven
at 60˚C for 30 minutes
7. Take out the recipient block when completely transparent
⇒ Embedding
8. Solidify the block in cold plate
9. Cutting
* In case of using 2, 3 and 5mm recipient block, insert the extracted tissue directly into the recipient
block without the guide by pushing the Quick-Ray™ knob down.
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
13. Tissue array using Quick-Ray™
BLOCK & SLIDE
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com
14. Tissue array using Quick-Ray™
ORDER
Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea
TEL 82-2-420-0070. FAX 82-2-420-9797
E-mail unitma@unitma.com http://www.unitma.com