Laboratory Decontamination
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Managing and maintaining the integrity of laboratories is challenging, particularly if contamination is discovered. During this webinar, you will learn about several decontamination and disinfection techniques used to decontaminate spaces and equipment.
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Laboratory Decontamination
- 1. Laboratory Decontamination A Step-by-Step Guide to Properly Cleaning a Laboratory
- 2. Meet Your Moderator James Ciccone
- 3. During this Webinar All lines will be muted – please communicate via the questions tab in your webinar panel. There will be a Q&A session at the end of the presentation – submit your question(s) anytime throughout the webinar. Webinar recording and slides will be emailed to you tomorrow. Stick around for an exclusive offer at the end of the webinar.
- 4. Meet Your Presenter Craig Sasse
- 5. Our Key Message Manage and eliminate contamination from the laboratory environment
- 6. Who Is This For? Researchers Ensuring integrity and protecting research Laboratory Managers Assuring equipment and procedures are suitable EH&S Managers Address problems in the laboratory space
- 7. Have you experienced contamination in your research?
- 8. What Will You Learn? How to prevent problems Maintaining a clean laboratory How to manage contamination
- 9. Agenda Contamination Sources Corrective & Preventative Actions Case Studies Summary & Questions
- 10. Contamination Sources Chemical Contaminants Chemical Hygiene Plan manages materials Sources of contamination are specific and readily monitored Contamination generally found on equipment and infrastructure Biological Contaminants Biological Safety Manual manages materials Sources of contamination are numerous Contamination in media and can readily migrate and proliferate
- 11. Chemical Sources Causes of contamination: • Spills • Malfunctioning equipment • Poor procedures and careless work • Worker exposures • Liability
- 12. Biologic Sources Causes of contamination: • Laboratory personnel • Poorly developed or poorly executed procedures Unclean lab coats, hand washing, excessive talk Mislabeling Cross contamination – pipettes, open media Poor cell culture management • Unclean/dirty work environment
- 13. Contamination Sources Corrective & Preventative Actions Case Studies Summary & Questions Agenda
- 14. Corrective and Preventive Action • Corrective action is intended to identify the root cause and the source of the problem and eliminate it in order to prevent occurrences. • Preventative action is proactive prediction to identify potential problems and attempts to avoid them.
- 15. Corrective and Preventive Action Cause • Inspection • Audit • Interviews Identify corrective action Develop preventative action Implementation
- 16. Corrective Corrective actions can vary widely examples are: • Disposing of contaminated media • Remediation of spills or contamination • Re-training, equipment modifications, process modifications
- 17. Prevention • Modify procedures, equipment, or environment • Risk and exposure assessments • Practicing aseptic techniques • Biosafety and chemical hygiene plan reviews and revisions • Review disinfection means and methods
- 18. Contamination Sources Corrective & Preventative Actions Case Studies Summary & Questions Agenda
- 19. Case Study 1: Mold Contamination Repeat mold contamination in broths and media Cleaning and disinfection, but contamination recurred Assessment identified mold inside walls from a water leak
- 20. The contamination wasted months of research Assessment and remediation was disruptive and costly Research resumed after remediation and the entire lab was disinfected Case Study 1: Mold Contamination
- 21. An unwillingness to fix the initial leak thoroughly caused contamination and costly remediation. Case Study 1: Mold Contamination
- 22. Case Study 2: Mycoplasma Contamination Researchers had discovered mycoplasma contamination in a cell culture Media was isolated, but contamination was found in other labs Initial controls included isolation, quarantining, disposal, decontamination
- 23. Case Study 2: Mycoplasma Contamination Reviewed work procedures and identified several contributing factors Aggressive disposal of media Reviewed training, aggressive disinfection and procedure revisions resolved problem
- 24. Case Study 3: Equipment Contamination Biological Safety Cabinets
- 25. Case Study 3: Equipment Contamination Incubators
- 26. Case Study 3: Equipment Contamination Fridge & Cold Rooms
- 27. Our Key Message Manage and eliminate contamination from the laboratory environment
- 28. Contamination Sources Corrective & Preventative Actions Case Studies Summary & Questions Agenda
- 29. Summary Consistent work practices and routine cleaning by appropriate means and methods will protect valuable research. Chemical handling and disposal practices are critical for safe and compliant work. Practice, practice and practice aseptic techniques. SOP’s (Standard Operating Procedures), CAPA (Corrective Action and Preventative Action), and internal audits and QA inspections
- 30. QUESTIONS?
- 31. Thank You For Attending! Call Us! 1-888-834-9697 www.triumvirate.com Request a Free Lab Decontamination Consultation: http://info.triumvirate.com/lab- decontamination-consultation Contact: Craig Sasse: csasse@triumvirate.com