Biosafety in Molecular Biology
•Download as PPTX, PDF•
0 likes•36 views
The Lecture tells about the basic precautions needed to be taken in molecular biology and microbiology lab.
Report
Share
Report
Share
Recommended
Laboratory safety
The document outlines the essential components of a laboratory safety program, including identifying biological, chemical, radiological, fire and electrical hazards; developing and implementing safety policies and procedures; and evaluating the program for regulatory compliance to protect workers, products, and the environment from laboratory hazards.
Laboratory safety
This document provides information on laboratory safety practices. It discusses designating a safety officer, biosafety levels and practices, general safety rules and regulations, biosafety cabinets, and safe use of biosafety cabinets. Biosafety levels range from level 1 to level 4, with increasing containment practices required for higher levels due to more dangerous biological agents. Biosafety cabinets come in classes I-III and types A1, A2, B1, and B2, with each type providing different levels of protection for samples and/or personnel. Proper use of biosafety cabinets is important to maintain safe airflow.
Occupational and bio safety in food industries
This document discusses occupational health, safety, and bio safety in food industries. It covers the following key points:
Occupational health aims to control health hazards and prevent work-related diseases and accidents. Workers face a wide range of physical, chemical, and biological hazards. Safety measures include proper training, protective equipment, ventilation, sanitation, and medical services.
Bio safety has four containment levels to safely handle infectious agents, based on hazard assessment. Elements of containment protect workers through laboratory practices and safety equipment, as well as facility design and construction. Risk-based guidelines specify the appropriate containment level depending on the pathogen type and transmission risk. Trainings help ensure safe laboratory procedures.
Lecture 3 - Biosafety Levels 1 & 2.pptx
1) The document summarizes biosafety levels 1 and 2, including laboratory design features, equipment requirements, and waste handling procedures to protect workers and the environment.
2) Key aspects of biosafety levels 1 and 2 include procedures to minimize aerosol production, requirements for personal protective equipment, and methods for decontaminating or sterilizing infectious materials within the laboratory before disposal.
3) Training of laboratory workers in safe microbiological techniques is essential to prevent the spread of pathogens, as human error can compromise safety systems.
Biosafety in the laboratories
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
Biosafety Levels by Francis and Prasoona .pptx
This document discusses biosafety and biosafety levels. It defines biosafety as steps taken to protect humans, products, and the environment from biological hazards that may occur from research or commerce involving infectious organisms or genetically modified organisms. The document then describes the four biosafety levels established by the CDC - Biosafety Level 1 requires basic safety precautions; Biosafety Level 2 requires more extensive precautions for work with pathogens that pose moderate hazards; Biosafety Level 3 is for dangerous indigenous or exotic agents that may cause severe disease through inhalation; and Biosafety Level 4 contains the most hazardous pathogens and requires the highest level of containment.
1. laboratory safety protocol
This document outlines the laboratory safety protocol for a microbiology laboratory course. It discusses the course assessments, which include quizzes, a midterm exam, and a final exam. It also describes the various biosafety levels based on the infectious agents being studied, with biosafety level 4 requiring the highest level of containment. The basic safety requirements for the microbiology laboratory are provided, including maintaining a clean work area, wearing protective clothing and closed-toe shoes, prohibiting food and drinks, proper pipetting techniques, hand washing, and autoclaving contaminated materials.
Safety procedure of lab
This presentation introduces engineering laboratory safety procedures. It defines a laboratory as a facility that provides controlled conditions for scientific research and experimentation. Engineering laboratories specifically promote innovation through measurement and testing of engineered systems. The goal of safety programs is to minimize risks by ensuring workers have necessary training, information, and protective equipment. Key safety procedures covered include following standard operating procedures, wearing appropriate personal protective equipment, prohibiting food and drink, safely transferring hazardous materials, securing compressed gases, restrictions on working alone, and maintaining laboratory security. Important labels are also highlighted.
Recommended
Laboratory safety
The document outlines the essential components of a laboratory safety program, including identifying biological, chemical, radiological, fire and electrical hazards; developing and implementing safety policies and procedures; and evaluating the program for regulatory compliance to protect workers, products, and the environment from laboratory hazards.
Laboratory safety
This document provides information on laboratory safety practices. It discusses designating a safety officer, biosafety levels and practices, general safety rules and regulations, biosafety cabinets, and safe use of biosafety cabinets. Biosafety levels range from level 1 to level 4, with increasing containment practices required for higher levels due to more dangerous biological agents. Biosafety cabinets come in classes I-III and types A1, A2, B1, and B2, with each type providing different levels of protection for samples and/or personnel. Proper use of biosafety cabinets is important to maintain safe airflow.
Occupational and bio safety in food industries
This document discusses occupational health, safety, and bio safety in food industries. It covers the following key points:
Occupational health aims to control health hazards and prevent work-related diseases and accidents. Workers face a wide range of physical, chemical, and biological hazards. Safety measures include proper training, protective equipment, ventilation, sanitation, and medical services.
Bio safety has four containment levels to safely handle infectious agents, based on hazard assessment. Elements of containment protect workers through laboratory practices and safety equipment, as well as facility design and construction. Risk-based guidelines specify the appropriate containment level depending on the pathogen type and transmission risk. Trainings help ensure safe laboratory procedures.
Lecture 3 - Biosafety Levels 1 & 2.pptx
1) The document summarizes biosafety levels 1 and 2, including laboratory design features, equipment requirements, and waste handling procedures to protect workers and the environment.
2) Key aspects of biosafety levels 1 and 2 include procedures to minimize aerosol production, requirements for personal protective equipment, and methods for decontaminating or sterilizing infectious materials within the laboratory before disposal.
3) Training of laboratory workers in safe microbiological techniques is essential to prevent the spread of pathogens, as human error can compromise safety systems.
Biosafety in the laboratories
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
Biosafety Levels by Francis and Prasoona .pptx
This document discusses biosafety and biosafety levels. It defines biosafety as steps taken to protect humans, products, and the environment from biological hazards that may occur from research or commerce involving infectious organisms or genetically modified organisms. The document then describes the four biosafety levels established by the CDC - Biosafety Level 1 requires basic safety precautions; Biosafety Level 2 requires more extensive precautions for work with pathogens that pose moderate hazards; Biosafety Level 3 is for dangerous indigenous or exotic agents that may cause severe disease through inhalation; and Biosafety Level 4 contains the most hazardous pathogens and requires the highest level of containment.
1. laboratory safety protocol
This document outlines the laboratory safety protocol for a microbiology laboratory course. It discusses the course assessments, which include quizzes, a midterm exam, and a final exam. It also describes the various biosafety levels based on the infectious agents being studied, with biosafety level 4 requiring the highest level of containment. The basic safety requirements for the microbiology laboratory are provided, including maintaining a clean work area, wearing protective clothing and closed-toe shoes, prohibiting food and drinks, proper pipetting techniques, hand washing, and autoclaving contaminated materials.
Safety procedure of lab
This presentation introduces engineering laboratory safety procedures. It defines a laboratory as a facility that provides controlled conditions for scientific research and experimentation. Engineering laboratories specifically promote innovation through measurement and testing of engineered systems. The goal of safety programs is to minimize risks by ensuring workers have necessary training, information, and protective equipment. Key safety procedures covered include following standard operating procedures, wearing appropriate personal protective equipment, prohibiting food and drink, safely transferring hazardous materials, securing compressed gases, restrictions on working alone, and maintaining laboratory security. Important labels are also highlighted.
Biosafety and biosecurity biosafety levels
This document discusses biosafety and biosecurity. It defines biosafety as containment principles and practices to prevent unintentional exposure to pathogens. This includes laboratory worker protection, containment design, guidelines and safe practices. It describes World Health Organization (WHO) risk groups 1-4 which categorize agents based on factors like pathogenicity. It also outlines biosafety levels 1-4 which are determined by composite factors including containment and procedures. The document emphasizes principles like risk assessment, training, and emergency response planning. It defines biosecurity as measures to prevent theft or intentional release of pathogens. Developing strong biosafety and biosecurity programs requires involvement from various stakeholders.
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...Guntamukkala Brahmayesu
This document discusses laboratory safety regulations and guidelines. It outlines the responsibilities of both employers and employees to maintain a safe work environment. Some of the main hazards identified in clinical laboratories include chemicals, biological specimens, fires, electricity, and compressed gases. The document recommends various safety practices and use of personal protective equipment. It also describes U.S. regulations regarding occupational safety, including OSHA, and guidelines from organizations like NIOSH, NFPA, and NCCLS. Biological safety practices for handling infectious specimens are emphasized.Biosafety ppt
Biosafety levels range from 1 to 4 based on the hazards posed by infectious agents, with level 1 posing minimal risk and level 4 the highest. Biosafety level 1 involves standard precautions for microbes not known to cause disease in healthy adults. Level 2 requires restricted access and personal protective equipment for work with moderate hazards. Level 3 involves serious diseases transmitted through respiratory routes and requires medical surveillance, immunizations, respirators and controlled lab access. Biosafety level 4 is the highest level involving dangerous exotic microbes and requires change of clothes, showering and separate containment facilities. Adherence to biosafety guidelines and regulations helps reduce laboratory risks.
Biosafety
There are four biosafety levels that provide increasing levels of containment for biological agents. Biosafety level 1 is for work with well-characterized agents that do not cause disease in healthy adults. It requires standard microbiological practices and personal protective equipment like lab coats and gloves. Biosafety level 2 involves agents that pose moderate hazards and requires all BSL-1 practices plus additional containment like restricted access and use of biosafety cabinets. Biosafety level 3 is for serious and potentially lethal agents and requires BSL-2 practices plus additional containment like controlled access, required personal protective equipment, and specialized facility design with airflow controls and separate rooms. Biosafety level 4 is the highest level and involves dangerous and exotic agents
safety precautions in laboratories pathology
Safety precautions in laboratories are important to protect both personnel and the environment from hazardous materials. Laboratories should create safety manuals covering hazards and mitigation plans, and train all staff on safety policies for things like biohazard disposal, chemical handling, and standard precautions. It is essential to identify chemical, physical, and biological hazards and implement controls like protective equipment, ventilation, emergency equipment, and proper chemical storage and disposal procedures to maintain a safe laboratory environment.
7.Laboratory safety and Biohazard
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
Safety measures, short and accurate ppt
The document discusses safety measures for clinical microbiology laboratories. It emphasizes the importance of lab safety to prevent adverse effects from potential hazards. Good lab practices include proper personal hygiene like handwashing and avoiding eating or drinking in the lab. Labs must follow biosafety guidelines for different levels of microorganisms. Aseptic technique is also important to avoid contamination. Proper clean up and disposal of materials helps maintain safety, such as autoclaving contaminated glassware before disposal.
Biosafty, containment and biosafty levels
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
Bio safety iitb 1
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
BIO SAFETY
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
مسلم.pptx
This document discusses biosafety in laboratories. It defines biosafety as safety precautions that reduce exposure risk to infectious materials. Biosafety is needed because laboratories work with hazardous infectious agents and accidents could threaten workers and the environment. There are four biosafety levels depending on the agent, with level 4 being the most dangerous exotic microbes like Ebola. Biosafety cabinets are important containment equipment that protect workers and prevent microbe release. The document also discusses biological waste disposal, decontamination, and controlling hazardous substances like chemicals under COSHH regulations through risk assessment and proper control measures.
Biosafety and Cartagena Protocol
Biosafety refers to ensuring safety when working with biological organisms. This document discusses biosafety concepts and issues including containment levels, biosafety cabinets, and risk assessment. The four biosafety levels range from level 1 posing minimal risk to level 4 posing high individual risk without vaccines or treatments. Biosafety cabinets are used to protect workers and the environment, with class I protecting environment, class II protecting samples and environment, and class III providing maximum protection in BSL-4 labs. Risk assessment considers an organism's pathogenicity, virulence, proliferation ability, and transmission route. Guidelines for recombinant DNA research emphasize risk-based containment and avoiding unnecessary regulation.
Good Laboratory Practices for Pharmaceutical Quality Control Laboratories
- Introduction - Requirements of GLP - Stepwise GLP in QC - Audit Findings - Issues due to not following GLP - Future Trends in GLP
Conducting Vivarium Risk Assessments in the Research Environment
This presentation addresses vivarium risk assessments for chemical and biological exposures in a research setting. Committee approval processes (IBC, IACUC, etc), controlling banding application, OEL development/validation, and IH monitoring results and control measures are just some of the topics presented.
Laboratory biosafety levels and transfer of biological samples
This document discusses laboratory biosafety levels and the transfer of biological samples. It begins by outlining the objectives and presentation outline. It then introduces biosafety practices and discusses the four biosafety levels - BSL-1 to BSL-4 - in increasing order of risk. Each level is associated with different safety practices and containment facilities depending on the risk of pathogens handled. The document also discusses regulations for the safe transfer of biological samples between facilities, including packaging, labeling and documentation requirements. Material transfer agreements are identified as important for governing the transfer and use of biological materials.
Biosafety - History, Levels and issues
This document provides an introduction to biosafety. It defines biosafety as safety from exposure to infectious agents according to the CDC. It then discusses the history of biosafety, including the first biosafety conference in 1955 and the establishment of biosafety levels 1 through 4 by the CDC. The document outlines the need for biosafety in laboratories working with infectious agents and describes the scope of biosafety across fields like medicine, agriculture and exobiology. It also covers biosafety issues in academic research, regulations, signage, hazardous materials, and provides details on the four biosafety levels based on pathogen risk.
Laboratory work, symptoms and specimen collecting
This document discusses laboratory work in microbiology, including the history and objectives of laboratory work. It describes guidelines for working with microorganisms, including their categorization into biosafety levels based on pathogenicity. It covers biosafety level practices and the engineering controls required for different biosafety levels. It also discusses specimen collection and handling for microbiology, including transport, rejection criteria, and standard precautions. Methods for clinical diagnosis in the microbiology laboratory include direct examination and culture/isolation.
Lab safety
The document discusses laboratory safety for clinical personnel. It outlines objectives around safety awareness, hazards, and responsibilities. Potential hazards include electric shock, toxic gases, radiation, and biological materials. Safety is achieved through recognition of hazards, good habits, and applying engineering controls, personal protective equipment, and work practice controls. Regulations aim to provide a safe work environment and are established by organizations like OSHA, NIOSH, DOT and others.
Different Biosafety levels of laboratory.ppt
The document discusses the history and key aspects of biological safety levels. It began with the first Biological Safety Conference in 1955 organized by the U.S. Army to share knowledge on bio-safety issues. In 1964, the CDC specified four biosafety levels (BSL 1-4) to isolate dangerous biological agents. BSL1 is for well-characterized agents of minimal hazard. BSL2 requires more containment for agents of moderate hazard. BSL3 is for indigenous or exotic agents that may cause serious disease, while BSL4 is for dangerous and exotic agents that pose high risk of life-threatening disease. The document outlines the standard and special practices, safety equipment and facilities required at each level.
SAR of Medicinal Chemistry 1st by dk.pdf
In this presentation include the prototype drug SAR on thus or with their examples .
Syllabus of Second Year B. Pharmacy
2019 PATTERN.
More Related Content
Similar to Biosafety in Molecular Biology
Biosafety and biosecurity biosafety levels
This document discusses biosafety and biosecurity. It defines biosafety as containment principles and practices to prevent unintentional exposure to pathogens. This includes laboratory worker protection, containment design, guidelines and safe practices. It describes World Health Organization (WHO) risk groups 1-4 which categorize agents based on factors like pathogenicity. It also outlines biosafety levels 1-4 which are determined by composite factors including containment and procedures. The document emphasizes principles like risk assessment, training, and emergency response planning. It defines biosecurity as measures to prevent theft or intentional release of pathogens. Developing strong biosafety and biosecurity programs requires involvement from various stakeholders.
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...Guntamukkala Brahmayesu
This document discusses laboratory safety regulations and guidelines. It outlines the responsibilities of both employers and employees to maintain a safe work environment. Some of the main hazards identified in clinical laboratories include chemicals, biological specimens, fires, electricity, and compressed gases. The document recommends various safety practices and use of personal protective equipment. It also describes U.S. regulations regarding occupational safety, including OSHA, and guidelines from organizations like NIOSH, NFPA, and NCCLS. Biological safety practices for handling infectious specimens are emphasized.Biosafety ppt
Biosafety levels range from 1 to 4 based on the hazards posed by infectious agents, with level 1 posing minimal risk and level 4 the highest. Biosafety level 1 involves standard precautions for microbes not known to cause disease in healthy adults. Level 2 requires restricted access and personal protective equipment for work with moderate hazards. Level 3 involves serious diseases transmitted through respiratory routes and requires medical surveillance, immunizations, respirators and controlled lab access. Biosafety level 4 is the highest level involving dangerous exotic microbes and requires change of clothes, showering and separate containment facilities. Adherence to biosafety guidelines and regulations helps reduce laboratory risks.
Biosafety
There are four biosafety levels that provide increasing levels of containment for biological agents. Biosafety level 1 is for work with well-characterized agents that do not cause disease in healthy adults. It requires standard microbiological practices and personal protective equipment like lab coats and gloves. Biosafety level 2 involves agents that pose moderate hazards and requires all BSL-1 practices plus additional containment like restricted access and use of biosafety cabinets. Biosafety level 3 is for serious and potentially lethal agents and requires BSL-2 practices plus additional containment like controlled access, required personal protective equipment, and specialized facility design with airflow controls and separate rooms. Biosafety level 4 is the highest level and involves dangerous and exotic agents
safety precautions in laboratories pathology
Safety precautions in laboratories are important to protect both personnel and the environment from hazardous materials. Laboratories should create safety manuals covering hazards and mitigation plans, and train all staff on safety policies for things like biohazard disposal, chemical handling, and standard precautions. It is essential to identify chemical, physical, and biological hazards and implement controls like protective equipment, ventilation, emergency equipment, and proper chemical storage and disposal procedures to maintain a safe laboratory environment.
7.Laboratory safety and Biohazard
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
Safety measures, short and accurate ppt
The document discusses safety measures for clinical microbiology laboratories. It emphasizes the importance of lab safety to prevent adverse effects from potential hazards. Good lab practices include proper personal hygiene like handwashing and avoiding eating or drinking in the lab. Labs must follow biosafety guidelines for different levels of microorganisms. Aseptic technique is also important to avoid contamination. Proper clean up and disposal of materials helps maintain safety, such as autoclaving contaminated glassware before disposal.
Biosafty, containment and biosafty levels
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
Bio safety iitb 1
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
BIO SAFETY
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
مسلم.pptx
This document discusses biosafety in laboratories. It defines biosafety as safety precautions that reduce exposure risk to infectious materials. Biosafety is needed because laboratories work with hazardous infectious agents and accidents could threaten workers and the environment. There are four biosafety levels depending on the agent, with level 4 being the most dangerous exotic microbes like Ebola. Biosafety cabinets are important containment equipment that protect workers and prevent microbe release. The document also discusses biological waste disposal, decontamination, and controlling hazardous substances like chemicals under COSHH regulations through risk assessment and proper control measures.
Biosafety and Cartagena Protocol
Biosafety refers to ensuring safety when working with biological organisms. This document discusses biosafety concepts and issues including containment levels, biosafety cabinets, and risk assessment. The four biosafety levels range from level 1 posing minimal risk to level 4 posing high individual risk without vaccines or treatments. Biosafety cabinets are used to protect workers and the environment, with class I protecting environment, class II protecting samples and environment, and class III providing maximum protection in BSL-4 labs. Risk assessment considers an organism's pathogenicity, virulence, proliferation ability, and transmission route. Guidelines for recombinant DNA research emphasize risk-based containment and avoiding unnecessary regulation.
Good Laboratory Practices for Pharmaceutical Quality Control Laboratories
- Introduction - Requirements of GLP - Stepwise GLP in QC - Audit Findings - Issues due to not following GLP - Future Trends in GLP
Conducting Vivarium Risk Assessments in the Research Environment
This presentation addresses vivarium risk assessments for chemical and biological exposures in a research setting. Committee approval processes (IBC, IACUC, etc), controlling banding application, OEL development/validation, and IH monitoring results and control measures are just some of the topics presented.
Laboratory biosafety levels and transfer of biological samples
This document discusses laboratory biosafety levels and the transfer of biological samples. It begins by outlining the objectives and presentation outline. It then introduces biosafety practices and discusses the four biosafety levels - BSL-1 to BSL-4 - in increasing order of risk. Each level is associated with different safety practices and containment facilities depending on the risk of pathogens handled. The document also discusses regulations for the safe transfer of biological samples between facilities, including packaging, labeling and documentation requirements. Material transfer agreements are identified as important for governing the transfer and use of biological materials.
Biosafety - History, Levels and issues
This document provides an introduction to biosafety. It defines biosafety as safety from exposure to infectious agents according to the CDC. It then discusses the history of biosafety, including the first biosafety conference in 1955 and the establishment of biosafety levels 1 through 4 by the CDC. The document outlines the need for biosafety in laboratories working with infectious agents and describes the scope of biosafety across fields like medicine, agriculture and exobiology. It also covers biosafety issues in academic research, regulations, signage, hazardous materials, and provides details on the four biosafety levels based on pathogen risk.
Laboratory work, symptoms and specimen collecting
This document discusses laboratory work in microbiology, including the history and objectives of laboratory work. It describes guidelines for working with microorganisms, including their categorization into biosafety levels based on pathogenicity. It covers biosafety level practices and the engineering controls required for different biosafety levels. It also discusses specimen collection and handling for microbiology, including transport, rejection criteria, and standard precautions. Methods for clinical diagnosis in the microbiology laboratory include direct examination and culture/isolation.
Lab safety
The document discusses laboratory safety for clinical personnel. It outlines objectives around safety awareness, hazards, and responsibilities. Potential hazards include electric shock, toxic gases, radiation, and biological materials. Safety is achieved through recognition of hazards, good habits, and applying engineering controls, personal protective equipment, and work practice controls. Regulations aim to provide a safe work environment and are established by organizations like OSHA, NIOSH, DOT and others.
Different Biosafety levels of laboratory.ppt
The document discusses the history and key aspects of biological safety levels. It began with the first Biological Safety Conference in 1955 organized by the U.S. Army to share knowledge on bio-safety issues. In 1964, the CDC specified four biosafety levels (BSL 1-4) to isolate dangerous biological agents. BSL1 is for well-characterized agents of minimal hazard. BSL2 requires more containment for agents of moderate hazard. BSL3 is for indigenous or exotic agents that may cause serious disease, while BSL4 is for dangerous and exotic agents that pose high risk of life-threatening disease. The document outlines the standard and special practices, safety equipment and facilities required at each level.
Similar to Biosafety in Molecular Biology (20)
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Good Laboratory Practices for Pharmaceutical Quality Control Laboratories
Good Laboratory Practices for Pharmaceutical Quality Control Laboratories
Conducting Vivarium Risk Assessments in the Research Environment
Conducting Vivarium Risk Assessments in the Research Environment
Laboratory biosafety levels and transfer of biological samples
Laboratory biosafety levels and transfer of biological samples
Gp 2 - Good Lab Practices for Microbiology Lab.pptx
Gp 2 - Good Lab Practices for Microbiology Lab.pptx
Recently uploaded
SAR of Medicinal Chemistry 1st by dk.pdf
In this presentation include the prototype drug SAR on thus or with their examples .
Syllabus of Second Year B. Pharmacy
2019 PATTERN.
bordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
Shallowest Oil Discovery of Turkiye.pptx
The Petroleum System of the Çukurova Field - the Shallowest Oil Discovery of Türkiye, Adana
Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero WaterTexas Alliance of Groundwater Districts
Presented at June 6-7 Texas Alliance of Groundwater Districts Business MeetingRandomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...Abdul Wali Khan University Mardan,kP,Pakistan
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills原版制作(carleton毕业证书)卡尔顿大学毕业证硕士文凭原版一模一样
原版纸张【微信：741003700 】【(carleton毕业证书)卡尔顿大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/Cytokines and their role in immune regulation.pptx
This presentation covers the content and information on "Cytokines " and their role in immune regulation .
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Thornton ESPP slides UK WW Network 4_6_24.pdf
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
Recently uploaded (20)
bordetella pertussis.................................ppt
bordetella pertussis.................................ppt
20240520 Planning a Circuit Simulator in JavaScript.pptx
20240520 Planning a Circuit Simulator in JavaScript.pptx
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...
Cytokines and their role in immune regulation.pptx
Cytokines and their role in immune regulation.pptx
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
The debris of the ‘last major merger’ is dynamically young
The debris of the ‘last major merger’ is dynamically young
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Equivariant neural networks and representation theory
Equivariant neural networks and representation theory
Biosafety in Molecular Biology
- 2. Molecular Biology Lab • Advanced understanding of biological processes • Several biohazards needing containment • Principles of containment include safe methods, facilities, equipment. • Biosafety will ensure any potential exposure with hazardous substances.
- 3. Laboratory Practice and Education • Workers must be trained and educated to deal the potential hazards. • Proficient to use lab equipment, chemicals and infectious substances.
- 4. Work and Risk Assessment • Identification of potential risks • Classification of risks in risk groups. • Compliance with standard microbiological practices. • Prohibition of mouth pipetting. • Washing and sanitizing hands. • Safe handling of sharps according to practice control measures. • Decontamination of surface and equipment. • Waste decontamination and disposal.
- 5. • Electrocution • Eyewash station • Display and dispatch biosafety manual • Biosafety labels and signs • Pest management • Material safety datasheets for chemicals • Doors for access control • Safety cabinets
- 6. • PPEs • Biosafety cabinets • Vaccination of lab workers • Class 1 Cabinets • Inward airflow protects workers • Exhaust to outside with HEPA filters
- 7. • Class 2 Cabinets • Protection of worker, product, environment • Used with aerosol transmissible organisms, chemicals • Used for microorganisms and tissue culture • Class 3 Cabinets • Totally enclosed • Ventilated • Air-tight • Usually for BSL 3 and 4
- 8. • Transportation of samples through authorized commercial entities • Precautions on Primate cells and Cell Lines