This document summarizes information about infectious haematopoietic necrosis (IHN), a viral disease affecting salmonid fish. IHN is caused by the fish rhabdovirus IHNV. It primarily affects rainbow trout farms, where it can cause high mortality rates in acute outbreaks. IHNV has a single-stranded RNA genome and infects hematopoietic tissues like the kidney and spleen. Clinical signs include darkening of the skin, exophthalmia, and hemorrhaging. Diagnosis involves examining tissue imprints for necrobiotic bodies and detecting the virus through electron microscopy, which reveals bullet-shaped virions in infected cells.
Infectious hematopoietic necrosis virus in finfish (IHNV)Avijit Pramanik
Infectious hematopoietic necrosis (IHN) is a viral disease affecting salmonid fish that was first recognized in the 1950s. The causative agent is the IHN virus (IHNV), a bullet-shaped RNA virus from the genus Novirhabdovirus. IHNV spreads horizontally between fish and vertically through eggs. Clinical signs include darkening of skin, exophthalmia, and hemorrhaging. Diagnosis involves identifying necrotic cells in kidney smears, detecting the virus via RT-PCR or immunochemistry, and observing histopathological changes in tissues. Prevention focuses on good hygiene and using virus-free water supplies.
Viral haemorrhagic septicaemia (VHS) is caused by infection with viral haemorrhagic septicaemia virus (VHSV), which is a rhabdovirus that infects both farmed and wild fish species. VHS causes hemorrhaging and high mortality in infected fish. The virus is transmitted horizontally between fish via contaminated water. While there is no approved vaccine, control methods focus on surveillance and culling infected populations to prevent transmission.
The document discusses various protozoan parasites that can infect shellfish, including Vorticella, Epistylis, Zoothamnium, Acineta, and Ephelota. It describes the appearance and effects of each protozoan, as well as symptoms they can cause like fuzzy growths on shells and gills. Maintaining good water quality and avoiding stressors can help prevent protozoan infections in shellfish farms.
COMMON VIRAL DISEASES OF FISHES AND SHRIMP IN BANGLADESH As Siyam
This document provides an overview of common viral diseases affecting fishes and shrimp in Bangladesh. It discusses 14 different viral diseases including betanodavirus, channel catfish virus, cyprinid herpesvirus 3, infectious hematopoietic necrosis virus, lymphocystis, ranavirus, snakehead rhabdovirus, viral hemorrhagic septicemia, and walleye epidermal hyperplasia virus. For each disease, it describes the causative virus, affected host species, clinical signs and symptoms, and methods of diagnosis. The document is intended as a presentation for an academic course on viral diseases of aquatic animals.
Broodstock And Hatchery Management Of Penaeus Monodonsush_p
Shrimp aquaculture is an important and valuable production sector that has been growing rapidly over the past two decades. Success is largely based on the quality of post larvae, particularly their health condition, thus making hatchery production of quality post larvae crucial to the sector’s sustainability. Vietnam is the leading producer of black tiger shrimp in the world with a production of 300,000 tons in 2011, followed by India and Indonesia with a production of 187,900 tons and 126,200 tons respectively.
Major contribution of the tiger shrimp to global shrimp production and the economic losses resulting from disease outbreaks, it is essential that the shrimp-farming sector invest in good management practices for the production of healthy and quality seed. The Indian shrimp hatchery industry has established a detailed guidance and protocols for improving the productivity, health management, biosecurity and sustainability of the sector. Following a brief review of shrimp hatchery development in India, the major requirements for hatchery production are discussed under the headings: infrastructure, facility maintenance, inlet water quality and treatment, wastewater treatment, biosecurity, standard operating procedures (SOPS), the Hazard Analysis Critical Control Point (HACCP) approach, chemical use during the hatchery production process and health assessment. Pre-spawning procedures include the use of wild, domesticated and specific pathogen free/ specific pathogen resistant (SPF/SPR) broodstock, broodstock selection and holding techniques, transport, utilization, health screening, maturation, nutrition and spawning, egg hatching; nauplius selection, egg/ nauplius disinfection and washing and holding, disease testing and transportation of nauplii. Post-spawning procedures include: larval-rearing unit preparation, larval rearing/health management, larval nutrition and feed management, important larval diseases, quality testing/selection of PL for stocking, PL harvest and transportation, nursery rearing and record keeping.
Ichthyophthirius multifiliis is a parasitic protozoan that causes white spot disease in freshwater fish. It has a direct life cycle with three stages: the feeding trophont stage on the fish, the reproducing tomont stage in the environment, and the infective theront stage. Clinical signs include white spots on the skin and fins. Diagnosis is made by microscopic examination of spots and seeing the characteristic moving trophonts. Common treatments include formalin, malachite green, increased temperature, or salt, with the goal of targeting the free-living theront stage.
Viral diseases that commonly infect fishes include viral hemorrhagic septicemia (VHS), infectious pancreatic necrosis (IPN), spring viraemia of carp (SVC), channel catfish virus (CCV), and infectious hematopoietic necrosis. These viruses are transmitted between fishes through water and infected eggs. Diseased fishes show symptoms like hemorrhaging, skin discoloration, organ damage, and abnormal swimming behaviors. Diagnosis involves virus isolation, antibody tests, and PCR. There are no treatments, so prevention focuses on hygiene, quarantine, avoiding stress, and inactivating viruses in the environment.
Infectious hematopoietic necrosis virus in finfish (IHNV)Avijit Pramanik
Infectious hematopoietic necrosis (IHN) is a viral disease affecting salmonid fish that was first recognized in the 1950s. The causative agent is the IHN virus (IHNV), a bullet-shaped RNA virus from the genus Novirhabdovirus. IHNV spreads horizontally between fish and vertically through eggs. Clinical signs include darkening of skin, exophthalmia, and hemorrhaging. Diagnosis involves identifying necrotic cells in kidney smears, detecting the virus via RT-PCR or immunochemistry, and observing histopathological changes in tissues. Prevention focuses on good hygiene and using virus-free water supplies.
Viral haemorrhagic septicaemia (VHS) is caused by infection with viral haemorrhagic septicaemia virus (VHSV), which is a rhabdovirus that infects both farmed and wild fish species. VHS causes hemorrhaging and high mortality in infected fish. The virus is transmitted horizontally between fish via contaminated water. While there is no approved vaccine, control methods focus on surveillance and culling infected populations to prevent transmission.
The document discusses various protozoan parasites that can infect shellfish, including Vorticella, Epistylis, Zoothamnium, Acineta, and Ephelota. It describes the appearance and effects of each protozoan, as well as symptoms they can cause like fuzzy growths on shells and gills. Maintaining good water quality and avoiding stressors can help prevent protozoan infections in shellfish farms.
COMMON VIRAL DISEASES OF FISHES AND SHRIMP IN BANGLADESH As Siyam
This document provides an overview of common viral diseases affecting fishes and shrimp in Bangladesh. It discusses 14 different viral diseases including betanodavirus, channel catfish virus, cyprinid herpesvirus 3, infectious hematopoietic necrosis virus, lymphocystis, ranavirus, snakehead rhabdovirus, viral hemorrhagic septicemia, and walleye epidermal hyperplasia virus. For each disease, it describes the causative virus, affected host species, clinical signs and symptoms, and methods of diagnosis. The document is intended as a presentation for an academic course on viral diseases of aquatic animals.
Broodstock And Hatchery Management Of Penaeus Monodonsush_p
Shrimp aquaculture is an important and valuable production sector that has been growing rapidly over the past two decades. Success is largely based on the quality of post larvae, particularly their health condition, thus making hatchery production of quality post larvae crucial to the sector’s sustainability. Vietnam is the leading producer of black tiger shrimp in the world with a production of 300,000 tons in 2011, followed by India and Indonesia with a production of 187,900 tons and 126,200 tons respectively.
Major contribution of the tiger shrimp to global shrimp production and the economic losses resulting from disease outbreaks, it is essential that the shrimp-farming sector invest in good management practices for the production of healthy and quality seed. The Indian shrimp hatchery industry has established a detailed guidance and protocols for improving the productivity, health management, biosecurity and sustainability of the sector. Following a brief review of shrimp hatchery development in India, the major requirements for hatchery production are discussed under the headings: infrastructure, facility maintenance, inlet water quality and treatment, wastewater treatment, biosecurity, standard operating procedures (SOPS), the Hazard Analysis Critical Control Point (HACCP) approach, chemical use during the hatchery production process and health assessment. Pre-spawning procedures include the use of wild, domesticated and specific pathogen free/ specific pathogen resistant (SPF/SPR) broodstock, broodstock selection and holding techniques, transport, utilization, health screening, maturation, nutrition and spawning, egg hatching; nauplius selection, egg/ nauplius disinfection and washing and holding, disease testing and transportation of nauplii. Post-spawning procedures include: larval-rearing unit preparation, larval rearing/health management, larval nutrition and feed management, important larval diseases, quality testing/selection of PL for stocking, PL harvest and transportation, nursery rearing and record keeping.
Ichthyophthirius multifiliis is a parasitic protozoan that causes white spot disease in freshwater fish. It has a direct life cycle with three stages: the feeding trophont stage on the fish, the reproducing tomont stage in the environment, and the infective theront stage. Clinical signs include white spots on the skin and fins. Diagnosis is made by microscopic examination of spots and seeing the characteristic moving trophonts. Common treatments include formalin, malachite green, increased temperature, or salt, with the goal of targeting the free-living theront stage.
Viral diseases that commonly infect fishes include viral hemorrhagic septicemia (VHS), infectious pancreatic necrosis (IPN), spring viraemia of carp (SVC), channel catfish virus (CCV), and infectious hematopoietic necrosis. These viruses are transmitted between fishes through water and infected eggs. Diseased fishes show symptoms like hemorrhaging, skin discoloration, organ damage, and abnormal swimming behaviors. Diagnosis involves virus isolation, antibody tests, and PCR. There are no treatments, so prevention focuses on hygiene, quarantine, avoiding stress, and inactivating viruses in the environment.
This document discusses branchiomycosis, also known as gill rot, which is a fungal disease caused by Branchiomyces sanguinis and Branchiomyces demigrans. It affects the gill tissues of many freshwater fish species. The fungi penetrate the gills, causing obstruction, congestion, and necrosis. Infected fish exhibit weakened movement, respiratory distress, and pale or red discolored gills. The disease spreads rapidly in warm water and can cause high mortality rates in affected fish populations. Treatment involves strict sanitation, drying and disinfecting infected ponds, and treating diseased fish with antifungal medications like malachite green.
Fish disease is a major constraint to aquaculture development in Bangladesh. Common diseases include bacterial, fungal, parasitic and physical ailments. The risk of disease outbreaks increases with intensification of aquaculture and high stocking densities. Proper management practices like monitoring health, controlling transboundary movements, training workers and utilizing disease prevention techniques can help control disease spread and its impacts on aquaculture.
Viral diseases pose a major threat to crustacean farming. This document summarizes several important viral diseases affecting farmed shrimp species. Yellow Head Disease, caused by Yellow Head Virus, causes mass mortalities in Penaeus monodon. White Spot Disease, caused by White Spot Syndrome Virus, infects juveniles of many shrimp species and can cause 80-100% mortality within a week. Taura Syndrome, caused by Taura Syndrome Virus, most severely affects Litopenaeus vannamei and is characterized by reddish discoloration and lesions. These and other viruses like Infectious Hypodermal and Hematopoietic Necrosis Virus, Baculoviral Midg
Fungal diseases can seriously impact fish populations. Three common fungal diseases are:
1. Saprolegniasis is caused by Saprolegnia fungi and is characterized by cotton-like fungal growths on the skin, gills, or eyes of fish. It can spread rapidly between fish and cause death.
2. Branchiomycosis (gill rot) infects gill tissues and is caused by Branchiomyces fungi. Infected fish have difficulty breathing and their gills may appear red.
3. Ichthyophonosis causes rough skin and white lesions inside the body and is caused by Ichthyophonus fungi. More severe infections result in organ
This document discusses disease management in aquaculture. It notes that diseases can cause 10-15% losses in production and maintaining proper water quality parameters is important to prevent disease. It outlines major diseases affecting freshwater pond culture like parasites, fungi, bacteria, and algal blooms. Common disease symptoms in fish include loss of appetite, abnormal behavior, skin lesions, and gill discoloration. The document provides recommendations to prevent disease through best management practices like maintaining water quality, using certified seed, monitoring fish health, and contacting experts if diseases are observed.
Introduction
Fish Health Management GOALS
Principles of fish health management
Factors affecting fish health
Common symptoms of diseases
General preventive measures
Proper Health Management through Manipulating the disease triangle
Conclusion
References
1. SPF animals are free from specific pathogens but may still be susceptible to infection. SPR animals have been selectively bred for resistance to particular pathogens through challenge testing.
2. Non-SPF broodstock can introduce novel diseases and pass pathogens to offspring without strict biosecurity. They may not have been selectively bred.
3. True SPF status requires rigorous screening and production in biosecure facilities; outside these facilities animals may still be disease-free but are no longer considered SPF.
This document discusses infectious pancreatic necrosis (IPN), a viral disease that affects young salmonids. It is caused by the IPN virus, a birnavirus with two RNA segments. The disease causes high mortality in fry and fingerlings. While it was first described in Canada in 1941, it has since spread worldwide and can be transmitted through infected water, equipment, fish, eggs and carriers like birds. Clinical signs include swimming abnormalities, loss of appetite and paleness. Diagnosis involves detecting viral lesions on the pancreas and intestines. Management requires controlling water quality, screening broodstock, disinfecting eggs and vaccinating fish. Many fish species can be infected and act as asymptomatic carriers.
This document discusses four major viral diseases that infect fish: viral hemorrhagic septicemia (VHS), infectious pancreatic necrosis (IPN), spring viremia of carp (SVC), and channel catfish viral disease (CCVD). It describes the causative viruses, transmission methods, common symptoms like hemorrhaging and pop-eyes, diagnosis techniques including virus isolation and PCR, and lack of effective treatments other than controlling water quality and fish stocking densities.
Costiosis, also known as blue slime disease, is caused by the protozoan parasite Ichthyobodo necator. The parasite infects fish through the skin and gills, attaching and feeding on epithelial cells. This causes skin irritation and hyperplasia, increased mucus production, and respiratory distress in the fish. Heavily infected fish appear lethargic with damaged fins and are often unable to swim normally. The parasite can be diagnosed by examining skin or gill biopsies microscopically. Treatment involves salt baths, formalin baths, or raising water temperature above 30 degrees Celsius to kill the parasites. Proper tank management can also help prevent outbreaks.
This document provides an overview of probiotics in aquaculture. It defines probiotics and discusses their history and uses. Probiotics are microorganisms that provide health benefits when consumed. They can be used as an alternative to antibiotics in aquaculture. The document outlines the characteristics of good probiotics, how they work to promote gut and immune health, and their benefits over antibiotics. It also discusses types of probiotics, methods of application, and recent findings on their use in fish and shrimp farming to improve growth, survival, and disease resistance.
Fundamentals of Aquarium Setting for Beginners. Historic
Social
Aesthetic and ornamental
Meditating
Commercial
Educational, Scientific and Research
Setting up of aquarium
Maintenance of aquarium
Accessories required
Decorative used
Types of fish
DESIGN AND CONSTRUCTION OF SHRIMP HATCHERYKartik Mondal
Shrimp is a valuable aquatic food resources high In protein and command good export markets. The tiger shrimp P.indicus and p.merguiensis are important candidate or aquafarming.The seed requirement presently meet from the wild and insufficient to meet growing demand. Hence there is a good potential for hatchery production of shrimp seed.
The success of a shrimp hatchery depend on:
1.The choice of suitable site
2.Effectiveness and efficiency of the hatchery design
3.Experience of hatchery technicians
4.Efficiency of operational management.
This document provides information on major fungal diseases that affect shrimp farming. It discusses three main diseases: larval mycosis caused by fungi like Lagenidium and Sirolpidium which infect shrimp larvae; black gill disease caused by the fungus Fusarium solani which causes black spots on gills and lesions; and aflatoxicosis or "red disease" caused by toxins produced by Aspergillus fungi contaminating feed which causes reddening and death of infected shrimp. Prevention and treatment methods are outlined for each disease.
This document discusses feed formulation and processing. Feed formulation involves combining ingredients to meet animal production goals in a balanced, palatable, and stable manner. The formulation process considers available local ingredients, nutrient contents, animal requirements, and physical limitations. Feed processing alters ingredients physically and sometimes chemically to improve utilization, mixing, and stability. Key processing steps include weighing, grinding, mixing, autoclaving, pelletizing, drying, packaging, and storage. The overall goal is to produce a cost-effective feed that meets regional animal nutritional needs.
Whiteleg shrimp (Litopenaeus vannamei, formerly Penaeus vannamei), also known as Pacific white shrimp or King prawn, is a variety of prawn of the eastern Pacific Ocean commonly caught or farmed for food.L. vannamei is a decapod crustacean which is native to the Eastern Pacific Coast of Central and
South America from Tumbes, Peru in the south to Mexico in the north. It has been introduced widely around the world since the 1970s, but especially since 2000, as it has become the principle
cultured shrimp species in Asia. The species itself is not considered a major threat to biodiversity, does not appear to have formed breeding populations, and has generally resulted in positive economic impacts in non-indigenous areas. An examination of current lists of invasive species
published by the International Union for Conservation of Nature’s Invasive Species Specialist Group (IUCN, 2004) revealed no listings for L. vannamei. As mentioned, L. vannamei has been anthropogenically introduced as an aquaculture species to several areas of the world to which it is
not native.
,
Nazmul Haque Syekat
This document provides information on diagnosing diseases in aquaculture. It discusses the importance of understanding normal conditions to detect abnormalities. The diagnosis process aims to determine the accurate cause of disease to select proper treatment. Key steps include taking a case history, conducting clinical, post-mortem, laboratory, histopathological, and molecular examinations. Signs of disease may be behavioral, physical on external/internal examination. Mortality can occur in acute outbreaks or chronic patterns over time. Accurate diagnosis is needed to effectively treat diseases affecting aquaculture.
This seminar discusses protozoan diseases that affect fishes. It is divided into three categories: 1) Flagellated protozoans like Ichthyobodo necatur, which attaches to gills of fish causing necrosis; 2) Ciliated protozoans like Ichthyophthirius multifilis and Trichodina sp. that live on gills and skin and can cause white spots; and 3) Myxozoans like Myxobolus cerebralis, which causes whirling disease in salmonids by destroying the inner ear. Key life cycles and symptoms are described for important diseases. Control involves prevention, chemotherapy, or raising water temperature for treatment.
This document discusses the culture methods for the rotifer Brachionus plicatilis, an important live feed for larval marine animals. It describes how to maintain stock cultures in 1-2L flasks using Isochrysis galbana algae at 24-25C under a 12:12 light cycle. Main culture methods discussed are using various tank sizes from 50L to 1800L, sterilizing tanks with bleach, and maintaining temperature at 24-26C with lighting. Feeding protocols are provided using yeast, fish oil, and algae to culture rotifers from low to high densities. Rotifers are harvested daily and fed to larval fish starting at 3 days post hatch at 3
This document discusses fish diseases and control measures. It describes how fish can be affected by various pathogens like viruses, bacteria, fungi and parasites. Some common viral diseases discussed are Spring Viremia of Carp caused by Rhabdovirus, Swim Bladder Inflammation caused by SBI virus, and Viral Renal Disease of Eels. It also discusses Channel Catfish Viral Disease caused by Herpes virus and Pox Disease of Carps caused by Carp Pox virus. The document emphasizes the importance of basic knowledge of fish diseases for fish farmers and highlights how diseases can be controlled through proper pond maintenance and use of antibiotics.
The document discusses fish vaccination, including its importance for sustainable aquaculture, types of vaccines, and mechanisms of action. Vaccination provides an alternative to antibiotics for combating bacterial and viral diseases. Major types include whole organism vaccines using killed or attenuated pathogens, as well as purified subunit and recombinant vaccines. Development of new fish vaccines takes 5-8 years and involves disease characterization, laboratory testing, field trials, and licensing. Current commercially available vaccines protect against diseases such as furunculosis, vibriosis, and spring viraemia of carp.
This document discusses branchiomycosis, also known as gill rot, which is a fungal disease caused by Branchiomyces sanguinis and Branchiomyces demigrans. It affects the gill tissues of many freshwater fish species. The fungi penetrate the gills, causing obstruction, congestion, and necrosis. Infected fish exhibit weakened movement, respiratory distress, and pale or red discolored gills. The disease spreads rapidly in warm water and can cause high mortality rates in affected fish populations. Treatment involves strict sanitation, drying and disinfecting infected ponds, and treating diseased fish with antifungal medications like malachite green.
Fish disease is a major constraint to aquaculture development in Bangladesh. Common diseases include bacterial, fungal, parasitic and physical ailments. The risk of disease outbreaks increases with intensification of aquaculture and high stocking densities. Proper management practices like monitoring health, controlling transboundary movements, training workers and utilizing disease prevention techniques can help control disease spread and its impacts on aquaculture.
Viral diseases pose a major threat to crustacean farming. This document summarizes several important viral diseases affecting farmed shrimp species. Yellow Head Disease, caused by Yellow Head Virus, causes mass mortalities in Penaeus monodon. White Spot Disease, caused by White Spot Syndrome Virus, infects juveniles of many shrimp species and can cause 80-100% mortality within a week. Taura Syndrome, caused by Taura Syndrome Virus, most severely affects Litopenaeus vannamei and is characterized by reddish discoloration and lesions. These and other viruses like Infectious Hypodermal and Hematopoietic Necrosis Virus, Baculoviral Midg
Fungal diseases can seriously impact fish populations. Three common fungal diseases are:
1. Saprolegniasis is caused by Saprolegnia fungi and is characterized by cotton-like fungal growths on the skin, gills, or eyes of fish. It can spread rapidly between fish and cause death.
2. Branchiomycosis (gill rot) infects gill tissues and is caused by Branchiomyces fungi. Infected fish have difficulty breathing and their gills may appear red.
3. Ichthyophonosis causes rough skin and white lesions inside the body and is caused by Ichthyophonus fungi. More severe infections result in organ
This document discusses disease management in aquaculture. It notes that diseases can cause 10-15% losses in production and maintaining proper water quality parameters is important to prevent disease. It outlines major diseases affecting freshwater pond culture like parasites, fungi, bacteria, and algal blooms. Common disease symptoms in fish include loss of appetite, abnormal behavior, skin lesions, and gill discoloration. The document provides recommendations to prevent disease through best management practices like maintaining water quality, using certified seed, monitoring fish health, and contacting experts if diseases are observed.
Introduction
Fish Health Management GOALS
Principles of fish health management
Factors affecting fish health
Common symptoms of diseases
General preventive measures
Proper Health Management through Manipulating the disease triangle
Conclusion
References
1. SPF animals are free from specific pathogens but may still be susceptible to infection. SPR animals have been selectively bred for resistance to particular pathogens through challenge testing.
2. Non-SPF broodstock can introduce novel diseases and pass pathogens to offspring without strict biosecurity. They may not have been selectively bred.
3. True SPF status requires rigorous screening and production in biosecure facilities; outside these facilities animals may still be disease-free but are no longer considered SPF.
This document discusses infectious pancreatic necrosis (IPN), a viral disease that affects young salmonids. It is caused by the IPN virus, a birnavirus with two RNA segments. The disease causes high mortality in fry and fingerlings. While it was first described in Canada in 1941, it has since spread worldwide and can be transmitted through infected water, equipment, fish, eggs and carriers like birds. Clinical signs include swimming abnormalities, loss of appetite and paleness. Diagnosis involves detecting viral lesions on the pancreas and intestines. Management requires controlling water quality, screening broodstock, disinfecting eggs and vaccinating fish. Many fish species can be infected and act as asymptomatic carriers.
This document discusses four major viral diseases that infect fish: viral hemorrhagic septicemia (VHS), infectious pancreatic necrosis (IPN), spring viremia of carp (SVC), and channel catfish viral disease (CCVD). It describes the causative viruses, transmission methods, common symptoms like hemorrhaging and pop-eyes, diagnosis techniques including virus isolation and PCR, and lack of effective treatments other than controlling water quality and fish stocking densities.
Costiosis, also known as blue slime disease, is caused by the protozoan parasite Ichthyobodo necator. The parasite infects fish through the skin and gills, attaching and feeding on epithelial cells. This causes skin irritation and hyperplasia, increased mucus production, and respiratory distress in the fish. Heavily infected fish appear lethargic with damaged fins and are often unable to swim normally. The parasite can be diagnosed by examining skin or gill biopsies microscopically. Treatment involves salt baths, formalin baths, or raising water temperature above 30 degrees Celsius to kill the parasites. Proper tank management can also help prevent outbreaks.
This document provides an overview of probiotics in aquaculture. It defines probiotics and discusses their history and uses. Probiotics are microorganisms that provide health benefits when consumed. They can be used as an alternative to antibiotics in aquaculture. The document outlines the characteristics of good probiotics, how they work to promote gut and immune health, and their benefits over antibiotics. It also discusses types of probiotics, methods of application, and recent findings on their use in fish and shrimp farming to improve growth, survival, and disease resistance.
Fundamentals of Aquarium Setting for Beginners. Historic
Social
Aesthetic and ornamental
Meditating
Commercial
Educational, Scientific and Research
Setting up of aquarium
Maintenance of aquarium
Accessories required
Decorative used
Types of fish
DESIGN AND CONSTRUCTION OF SHRIMP HATCHERYKartik Mondal
Shrimp is a valuable aquatic food resources high In protein and command good export markets. The tiger shrimp P.indicus and p.merguiensis are important candidate or aquafarming.The seed requirement presently meet from the wild and insufficient to meet growing demand. Hence there is a good potential for hatchery production of shrimp seed.
The success of a shrimp hatchery depend on:
1.The choice of suitable site
2.Effectiveness and efficiency of the hatchery design
3.Experience of hatchery technicians
4.Efficiency of operational management.
This document provides information on major fungal diseases that affect shrimp farming. It discusses three main diseases: larval mycosis caused by fungi like Lagenidium and Sirolpidium which infect shrimp larvae; black gill disease caused by the fungus Fusarium solani which causes black spots on gills and lesions; and aflatoxicosis or "red disease" caused by toxins produced by Aspergillus fungi contaminating feed which causes reddening and death of infected shrimp. Prevention and treatment methods are outlined for each disease.
This document discusses feed formulation and processing. Feed formulation involves combining ingredients to meet animal production goals in a balanced, palatable, and stable manner. The formulation process considers available local ingredients, nutrient contents, animal requirements, and physical limitations. Feed processing alters ingredients physically and sometimes chemically to improve utilization, mixing, and stability. Key processing steps include weighing, grinding, mixing, autoclaving, pelletizing, drying, packaging, and storage. The overall goal is to produce a cost-effective feed that meets regional animal nutritional needs.
Whiteleg shrimp (Litopenaeus vannamei, formerly Penaeus vannamei), also known as Pacific white shrimp or King prawn, is a variety of prawn of the eastern Pacific Ocean commonly caught or farmed for food.L. vannamei is a decapod crustacean which is native to the Eastern Pacific Coast of Central and
South America from Tumbes, Peru in the south to Mexico in the north. It has been introduced widely around the world since the 1970s, but especially since 2000, as it has become the principle
cultured shrimp species in Asia. The species itself is not considered a major threat to biodiversity, does not appear to have formed breeding populations, and has generally resulted in positive economic impacts in non-indigenous areas. An examination of current lists of invasive species
published by the International Union for Conservation of Nature’s Invasive Species Specialist Group (IUCN, 2004) revealed no listings for L. vannamei. As mentioned, L. vannamei has been anthropogenically introduced as an aquaculture species to several areas of the world to which it is
not native.
,
Nazmul Haque Syekat
This document provides information on diagnosing diseases in aquaculture. It discusses the importance of understanding normal conditions to detect abnormalities. The diagnosis process aims to determine the accurate cause of disease to select proper treatment. Key steps include taking a case history, conducting clinical, post-mortem, laboratory, histopathological, and molecular examinations. Signs of disease may be behavioral, physical on external/internal examination. Mortality can occur in acute outbreaks or chronic patterns over time. Accurate diagnosis is needed to effectively treat diseases affecting aquaculture.
This seminar discusses protozoan diseases that affect fishes. It is divided into three categories: 1) Flagellated protozoans like Ichthyobodo necatur, which attaches to gills of fish causing necrosis; 2) Ciliated protozoans like Ichthyophthirius multifilis and Trichodina sp. that live on gills and skin and can cause white spots; and 3) Myxozoans like Myxobolus cerebralis, which causes whirling disease in salmonids by destroying the inner ear. Key life cycles and symptoms are described for important diseases. Control involves prevention, chemotherapy, or raising water temperature for treatment.
This document discusses the culture methods for the rotifer Brachionus plicatilis, an important live feed for larval marine animals. It describes how to maintain stock cultures in 1-2L flasks using Isochrysis galbana algae at 24-25C under a 12:12 light cycle. Main culture methods discussed are using various tank sizes from 50L to 1800L, sterilizing tanks with bleach, and maintaining temperature at 24-26C with lighting. Feeding protocols are provided using yeast, fish oil, and algae to culture rotifers from low to high densities. Rotifers are harvested daily and fed to larval fish starting at 3 days post hatch at 3
This document discusses fish diseases and control measures. It describes how fish can be affected by various pathogens like viruses, bacteria, fungi and parasites. Some common viral diseases discussed are Spring Viremia of Carp caused by Rhabdovirus, Swim Bladder Inflammation caused by SBI virus, and Viral Renal Disease of Eels. It also discusses Channel Catfish Viral Disease caused by Herpes virus and Pox Disease of Carps caused by Carp Pox virus. The document emphasizes the importance of basic knowledge of fish diseases for fish farmers and highlights how diseases can be controlled through proper pond maintenance and use of antibiotics.
The document discusses fish vaccination, including its importance for sustainable aquaculture, types of vaccines, and mechanisms of action. Vaccination provides an alternative to antibiotics for combating bacterial and viral diseases. Major types include whole organism vaccines using killed or attenuated pathogens, as well as purified subunit and recombinant vaccines. Development of new fish vaccines takes 5-8 years and involves disease characterization, laboratory testing, field trials, and licensing. Current commercially available vaccines protect against diseases such as furunculosis, vibriosis, and spring viraemia of carp.
This document provides a guide to fish anatomy and dissection. It describes the external features of fish including fins, nostrils, lateral line and eyes. It details the internal organs such as gills, heart, kidneys, liver, gonads and digestive system. Dissection procedures are outlined to examine key organs and systems. The roles of different organs in respiration, osmoregulation, circulation and reproduction are summarized.
1. Spring Viremia of Carp is caused by the Rhabdovirus carpio, an RNA virus that affects carp and other cyprinids.
2. The disease is acute and systemic, causing high mortality especially in temperatures between 11-13°C. Clinical signs include skin and organ hemorrhaging, bulging eyes, and darkened skin.
3. First identified in Yugoslavia in the 1970s, the virus has since spread throughout Europe, Asia, and North America, causing significant losses to fisheries. Young fish are most severely affected, though adults can also show symptoms.
The document discusses general signs and symptoms of diseases in fish. It covers bacterial, viral, fungal, parasitic, nutritional and genetic diseases. For bacterial diseases, common signs include reddened or frayed fins, open sores, and rapid breathing. Viral diseases can cause gastrointestinal issues, sneezing, and weight loss. Fungal infections present as white or gray growths on fins and skin. Parasitic diseases are signaled by inactivity, loss of appetite, and visible parasites. Nutritional deficiencies result in issues like poor growth or eye problems. Genetic diseases exhibit symptoms depending on the specific condition.
This document provides information on various phyla of parasites found in fish, including Platyhelminthes (cestodes and trematodes), Nematoda (nematodes), and Acanthocephala (thorny-headed worms). It describes their basic morphology, lifecycles, examples of types of parasites, and the pathology they can cause in different types of fish hosts. Common parasites discussed include tapeworms, flukes, roundworms, and spiny-headed worms.
1. The study isolated fungi from six freshwater fish species, finding fungal infection in Labeo rohita and Carassius auratus.
2. Aspergillus spp, Mucor sp, and Penicillium spp were isolated from the gills, fins, and skin of C. auratus. Only Aspergillus sp was isolated from the caudal fin of L. rohita.
3. The presence of toxigenic fungi like Aspergillus increases health risks for both humans and animals through potential mycotoxin production.
This document discusses ectoparasites that affect fish, including fish lice (Argulus), fish leeches, and anchor worms (Lernaea). It describes their physical characteristics, symptoms they cause in fish, and potential treatments. It also covers bacterial, fungal, and viral diseases, providing information on causative agents, common symptoms, and treatment options. Overall, the key to preventing fish disease and parasites is maintaining optimal water conditions, quarantining new fish, and not overstocking tanks.
This document contains information about an exam on fish diseases and management. It includes (1) a matching question about different fish diseases and their causes, (2) short note topics about biological oxygen demand, water intoxication, and more, and (3) questions asking to tabulate differences between various diseases. The document provides sample exam questions, short notes assignments, and information about fish diseases and management.
This document discusses bony fish, including their:
- Classification in the phylum Chordata and class Actinopterygii, with over 25,000 species.
- Key characteristics like presence of swim bladders, bones, bony scales, and fin rays.
- Two major subgroups based on tail type, skeleton composition, and scale features.
- Diverse body shapes adapted for different habitats like streams, coral reefs, or burrowing.
- Respiration via gills, circulation with a four-chambered heart, and buoyancy regulated by a swim bladder.
- Varied coloration serving functions like camouflage, communication, and pred
Non-Infectious Disease
Not caused by pathogens
Cannot be transmitted to other species
Malnutrition, Avitaminoses, Heavy Metals etc. are responsible
Risk factors:
Genetics
Life-style
Environmental factors
Genetic Risk Factors
Determined by genes
Familial Disease Tendency
Disease runs in species
Recessive gene disorders
Down syndrome
Born with extra chromosome
Sex-linked disorders
Linked to x chromosome (female)
Can be recessive in females
Color blindness, hemophilia, & muscular dystrophy
Viral diseases are caused by viruses entering the body and multiplying within cells. They spread through respiratory droplets, sexual contact, mosquito bites, and contaminated surfaces. Common viral diseases include the common cold, influenza, HIV/AIDS, chickenpox, and measles. Treatment focuses on relieving symptoms while the immune system fights the virus. Antiviral drugs may also be used in some cases to shorten illnesses.
This document discusses fungal diseases of medical importance. It begins by describing the morphology and classification of fungi, including molds, yeasts, and dimorphic fungi. It then covers several types of fungal infections caused by Candida albicans, including oral and esophageal candidiasis, vaginal candidiasis, and cutaneous and systemic candidiasis. It also discusses dermatophyte infections like tinea corporis, tinea cruris, tinea pedis, tinea versicolor, and onychomycosis. The document concludes by covering treatment options for candidiasis and dermatophyte infections like topical and oral antifungals.
The document discusses several viral diseases including herpes simplex virus types 1 and 2, varicella (chickenpox), herpes zoster (shingles), rubeola (measles), and epidemic parotitis (mumps). It provides details on the etiology, clinical features, treatment, and prognosis of each disease. The diseases are spread through respiratory droplets, contact with lesions, or sexual contact. Clinical features may include lesions, rashes, fever, and lymphadenopathy. Treatment involves antivirals, analgesics, and supportive care.
This document provides information on several viral diseases that affect finfishes:
Viral hemorrhagic septicemia (VHS) is caused by a rhabdovirus and causes internal bleeding and organ damage in infected fish. Infectious pancreatic necrosis (IPN) is caused by a birnavirus and mainly affects young salmonids, causing pancreatic lesions. Spring viraemia of carp is caused by a rhabdovirus and results in hemorrhaging, edema, and loss of balance in infected carp species. The document also briefly summarizes infectious hematopoietic necrosis, koi herpesvirus disease, and their signs, transmission, diagnosis and control.
The document summarizes common bacterial diseases that affect fish and shellfish. It discusses diseases caused by bacteria like Columnaris, Edwardsiellosis, Vibriosis, and Motile Aeromonad Septicemia. For each disease, it describes the causative agent, affected species, common signs and symptoms, diagnosis, and methods for prevention and control. The document provides an overview of important bacterial pathogens, the diseases they cause, and approaches for management of bacterial infections in aquaculture.
Oncorhynchus masou virus disease (OMVD) is caused by Salmonid herpesvirus 2 (SalHV-2), also known as Oncorhynchus masou virus (OMV). It causes skin ulcers, tumors, and hepatitis in salmon and trout in Japan. Young fish experience high mortality from systemic infection, while survivors often develop tumors around the mouth. Diagnosis involves isolating the virus from infected tissues using cell culture and confirming identity through PCR, neutralization tests, or immunofluorescence. The virus is transmitted through direct contact or water and can be controlled through culling, disinfection, and vaccination.
EHN is a systemic viral disease of fish caused by ranaviruses in the family Iridoviridae. It is highly fatal in redfin perch but rainbow trout are less susceptible. The virus causes necrosis in the liver, spleen, and kidney. Outbreaks typically result in mass mortality of juvenile fish. Diagnosis involves isolating and identifying the virus. There are currently no available vaccines or treatments.
This document discusses several viral diseases that affect fishes:
1) Infectious pancreatic necrosis (IPN) is caused by a birnavirus and affects the liver, spleen, and gall bladder of infected fish.
2) Viral haemorrhagic septicaemia (VHS) is caused by a rhabdovirus and results in anemia, swelling, and hemorrhaging in fish.
3) Infectious haematopoietic necrosis (IHN) is caused by a bullet-shaped virus and affects salmonids, causing weakness, dark coloration, and abdominal swelling.
4) Spring viraemia of carp (SVC) is caused by a rhabdov
Seabass Diseases and control measures by B.pptxB. BHASKAR
This document discusses major diseases affecting Asian seabass aquaculture. It describes several viral pathogens like infectious spleen and kidney necrosis disease caused by megalocytivirus, red sea bream iridovirus, and viral nervous necrosis. It also discusses bacterial diseases caused by pathogens like Tenacibaculum maritimum, pathogenic vibrios, Photobacterium damsela, Mycobacterium spp., and Streptococcus iniae. Control measures discussed include biosecurity, general management improvements, vaccination, and antibiotics when administered via feed.
The document provides information on fish health management including:
1. Common bacterial, viral, parasitic, and fungal diseases that affect fish and crustaceans such as vibriosis, monodon baculovirus disease, white spot syndrome, and saprolegniasis.
2. The causative agents and symptoms of important infectious bacterial diseases in fish including hemorrhagic septicemia caused by Aeromonas hydrophila, furunculosis caused by A. salmonicida, and saddleback disease caused by Flexibacter columnaris.
3. Nutritional diseases in fish that result from deficiencies of proteins, fatty acids, vitamins, and minerals and the signs associated with each.
4
This document summarizes fish-borne zoonotic diseases and focuses on vibriosis and cholera. It discusses how vibriosis is caused by the bacteria Vibrio aguillarum in fish and can cause human cholera. Cholera is an infectious disease transmitted through contaminated food or water and causes severe diarrhea that can lead to death by dehydration. The document traces the history of cholera pandemics and discusses the etiology, pathogenesis, clinical signs, treatment and prevention of both vibriosis in fish and cholera in humans.
Clonorchis sinensis, commonly known as the oriental liver fluke, is a parasite that infects the livers of humans. It is found primarily in Asia, where an estimated 30 million people are currently infected. People become infected by eating raw or undercooked freshwater fish that contain the parasite's larvae. The larvae hatch in the intestines and migrate to the bile ducts of the liver where they mature into adult flukes. Long-term infection can lead to liver inflammation, enlargement, and over time cirrhosis or cholangiocarcinoma if left untreated. Praziquantel is the drug of choice for treating infections.
This document provides an overview of clinical parasitology and classifications of human parasites. It discusses nematodes (roundworms), cestodes (tapeworms), trematodes (flukes), and protozoa. For nematodes, it describes key intestinal roundworms like Ascaris lumbricoides and Enterobius vermicularis, as well as tissue/blood nematodes such as filarial worms. It also discusses cestodes like Taenia solium and Diphyllobothrium latum, and provides brief summaries of parasites' life cycles, symptoms, and treatments.
Salmonellosis is one of the most common causes of diarrhea in adult horses, ranging from subclinical to severe and potentially fatal forms. It is caused by Salmonella bacteria which can be transmitted through the environment, feed, water, or direct contact. Clinical signs include fever, anorexia, diarrhea, and neutropenia. Diagnosis involves culturing the bacteria from feces or tissues. Treatment focuses on fluid therapy and antibiotics while prevention relies on biosecurity measures.
This document provides information about hereditary fish diseases presented by a student group. It discusses several hereditary diseases including dropsy caused by bacteria or viruses, fish tuberculosis caused by mycobacteria bacteria, deformities which can be hereditary or due to environmental factors, albinism which is a genetic lack of pigmentation, tumors and cancers which can be due to genetic or viral factors, and eye diseases like fish-eye disease which is an autosomal recessive condition caused by mutations in the LCAT gene. Causes, symptoms, diagnosis and treatments are described for each condition.
The document summarizes viral encephalopathy and retinopathy (VER), also known as viral nervous necrosis (VNN), which is caused by betanodavirus and affects many finfish and shellfish species. It describes the virus's characteristics, transmission, susceptible hosts, clinical signs, pathology, diagnosis and control methods. The virus is highly resistant in water and can survive for long periods, infecting fish through water or infected tissues. Clinical signs include abnormal swimming and high mortality rates especially in larvae. Diagnosis involves virus isolation in cell culture or molecular methods like PCR from nervous system tissues.
Aeromonas Postgraduate Seminar Maulana Azad Medical College DelhiSayantan Banerjee
This document provides information on the genus Aeromonas. It begins by defining key characteristics, such as being gram negative, oxidase and catalase positive, and facultatively anaerobic. It describes their morphology and discusses the 3 major species implicated in human disease. It then covers the clinical significance of Aeromonas, focusing on gastroenteritis, wound/soft tissue infections, sepsis, and other miscellaneous infections. The document concludes by describing laboratory techniques for diagnosis, including use of selective media and biochemical testing.
Pasteurellosis is a bacterial disease of marine and brackish water fish caused by Photobacterium damselae subsp. piscicida. It is characterized by white granulomatous lesions in the internal organs. The disease was first reported in white perch in the U.S. in 1963. It takes either an acute or chronic form. In the acute form, there are few clinical signs but the internal organs show necrosis. In the chronic form, the spleen and kidney have small white lesions. Susceptible species include yellowtail, red sea bream, and sea bass. Treatment involves antibiotics administered via food.
This document discusses various foodborne parasites that can infect humans through the consumption of raw or undercooked fish and seafood. It describes the life cycles of several parasites, which typically involve crustaceans or mollusks as first intermediate hosts, fish as second intermediate hosts, and mammals as final hosts. Parasites mentioned include the nematodes Anisakis simplex and Angiostrongylus cantonensis, the cestodes Diphyllobothrium latum and Diphyllobothrium pacificum, and the trematodes Clonorchis spp. and others. Symptoms of infection and treatment options are provided for some parasites. The transmission of these parasites is associated with foods like sushi
This document discusses several post-mortem examination findings from cattle and other livestock. It describes conditions like fungal placentitis, bovine viral diarrhea, salmonellosis, iodine deficiency causing goiter, spinal abscesses, liver abscesses, endocarditis, pneumonia caused by various pathogens, parasitic infections, ruminal acidosis, copper and other poisonings, and an emerging bleeding calf syndrome. It emphasizes taking a thorough case history and considering all possible etiologies when investigating disease outbreaks on farms.
The document discusses various microorganisms found in water and their roles. It describes that water is essential for life and acts as a medium for cellular reactions. It discusses different types of water bodies and habitats that contain diverse microbes like bacteria, viruses, protozoa, fungi, algae, and helminths. Many of these microorganisms can cause water-borne diseases if the water is contaminated. Proper treatment is needed to ensure water is safe for drinking and other uses. Maintaining good hygiene is important for preventing water-washed and water-related illnesses.
The document discusses various microorganisms found in water and their roles. It describes that water is essential for life and acts as a medium for cellular reactions. It then covers different types of water sources and habitats for microbes. Various microbes that can be found in water are discussed in detail including viruses, bacteria, protozoa, fungi, algae, and helminths. The roles of these microorganisms in water-borne diseases and their transmission are also summarized.
This document provides a guide to common poultry diseases compiled by Dr. R.M. Sajid. It lists the name of each disease, the causative agent, typical postmortem lesions, and clinical signs. It covers both viral diseases like Newcastle disease, infectious bursal disease, and avian influenza as well as bacterial diseases like fowl cholera, pullorum disease, and mycoplasmal diseases like Mycoplasma gallisepticum infection. The guide is intended to help with disease diagnosis by describing the key features of each poultry illness.
Similar to Infectious Heamopoietic Necrosis Virus (20)
1) Clonal selection theory states that when a B cell encounters its specific antigen, it is activated to multiply and produce clones that secrete antibodies against that antigen.
2) Effector cells like plasma cells and activated T cells carry out immune responses by secreting antibodies or performing cell-mediated responses.
3) Memory cells are also produced and provide faster secondary immune responses upon reexposure to the same antigen.
Cytokines are cell signaling molecules that allow communication between cells of the immune system. They can be classified into six families based on structural similarities and functions. The interleukin (IL) family includes pro-inflammatory cytokines like IL-1 and IL-2 that are secreted by macrophages and T cells respectively to induce immune responses. Chemokines are a subgroup of cytokines that mobilize immune cells between tissues by influencing cytoskeletal proteins and adhesion molecules. Cytokines can have pleiotropic, redundant, synergistic or antagonistic effects on different target cells. Their localized release and cell surface receptor binding allows for precise communication between immune cells.
Cytokines are proteins that act as chemical messengers and are produced by immune cells to mediate and regulate immune responses and inflammation. They exist in different classes including interleukins, interferons, chemokines, and tumor necrosis factor. Cytokines act by binding to specific receptors on target cells and triggering signaling pathways that alter gene expression. This allows cytokines to influence diverse biological functions such as immune cell development, activation and communication.
This document discusses coastal and marine tourism. It defines coastal and marine tourism as recreational activities involving travel away from one's community that have the marine environment or coastal zone as their focus or host. Examples are provided of coastal recreation activities like beach volleyball and marine activities like scuba diving. Coastal and marine tourism can provide economic benefits but also environmental impacts if not managed sustainably, such as pollution, damage to coral reefs, and disturbance of wildlife. The document outlines some of the challenges faced in coastal areas from the high population pressure and need for sustainable tourism development and management.
1) Man-made disasters are caused by identifiable human actions such as negligence or deliberate acts. They can result from industrial accidents, chemical/biological/radiological incidents, or other human factors like ignorance or carelessness.
2) Some examples of common man-made disasters mentioned are oil and chemical spills, power outages, terrorist attacks, and industrial fires and explosions. Specific incidents highlighted include the 1986 Chernobyl nuclear accident and Bhopal chemical disaster.
3) Precautions that can help mitigate man-made disasters include using protective equipment in chemical/radiological incidents, properly maintaining infrastructure to prevent industrial accidents, and increasing awareness to discourage negligence. Historical disasters like London's Killer Fog are
Geographical Indications (GI)
Types of GI
Why GI needs to be protected?
Advantages of GI
How are GIs Protected?
WIPO and GI
GI in India
Registration process
GI in Tamil Nadu
Water quality problems in Fishes-nitrogenous components and heavy metalsJuliet Abisha
This document discusses nitrogen components and metal toxicity in fish. It describes how ammonia, nitrite, and nitrate are produced through fish waste and feeding and can become toxic at high levels. Ammonia is particularly toxic and its toxicity depends on factors like temperature, pH, and dissolved oxygen. Heavy metals like copper, cadmium, mercury, and zinc can also poison fish in high amounts. Pesticides added to water supplies can acutely poison fish through nerve damage. Maintaining good water quality through filtration and monitoring nitrogen levels and toxins is important for fish health.
This document discusses protein classification, sources, and requirements. It classifies proteins based on their chemical composition (simple vs complex), shape (fibrous vs globular), and biological function. The main protein sources used in aquaculture are animal proteins like fish meal and plant proteins like soybean meal. Protein requirements vary by species and depend on factors like temperature, feeding rate, and genetic composition. Deficiencies can cause reduced growth, cataracts, and scoliosis.
The document discusses the complement system of teleost fish. It has three pathways - the classical pathway, lectin pathway, and alternative pathway. All three pathways involve a cascade of complement components that ultimately lead to the formation of the membrane attack complex (MAC) on pathogen surfaces. The MAC forms pores that lyse pathogens. The complement system also opsonizes pathogens and generates inflammatory peptides like C3a and C5a. Strict regulation is needed to prevent damage to host cells, mediated by factors such as C1 inhibitor, factor H, decay accelerating factor, and CD59.
Vibriosis is a prevalent bacterial disease affecting marine fish caused by Vibrio anguillarum. It occurs worldwide in cultured and wild fish, especially salmonids and perch-like fish, during late summer in shallow waters. Clinical signs include red spots, skin lesions, hemorrhaging, and inflammation. Diagnosis involves isolating the bacteria from lesions on agar plates and identifying it biochemically or through serological tests. Treatment involves antibiotics in feed but the disease often reappears after treatment. Prevention focuses on maintaining good water quality and husbandry practices.
The document summarizes Loop-mediated Isothermal Amplification (LAMP), a sensitive and efficient nucleic acid amplification technique. LAMP uses 4-6 primers that recognize 6 distinct regions on the target gene. The reaction proceeds at a constant temperature without thermal cycling. It produces billions of copies of target DNA in less than an hour. LAMP has applications in rapid diagnosis of diseases and identification of parasites due to its low cost, simplicity and speed compared to PCR.
Photobacterium damselae subsp. piscicida causes pasteurellosis, a serious bacterial disease affecting marine fish worldwide. It is characterized by white granulomatous lesions in internal organs. Key virulence factors include iron uptake systems, adhesion proteins, outer membrane proteins, and AIP56 which induces apoptosis in macrophages. The bacteria can resist serum killing through capsule production. Transmission is thought to occur through fish-to-fish contact or an invertebrate vector. Diagnosis involves culture and identification of the bacteria from lesions.
1. Inflammation is the body's protective response to injury or infection that involves blood vessels, immune cells, and chemical mediators.
2. Chemical mediators of inflammation include vasoactive amines, peptides, lipid mediators, cytokines, and enzymes that are released from immune cells and damaged tissues. These mediators cause changes like increased blood flow and vascular permeability.
3. The inflammatory response aims to destroy and isolate the injurious agent while facilitating tissue repair. Prolonged inflammation can lead to chronic disease if the trigger persists.
Food security exists when all people have access to sufficient nutritious food. Nearly 800 million people face hunger globally. India has a large population living in poverty and facing malnutrition, though poverty has declined in recent years. Agricultural production and exports have fluctuated in India from 2007-2010. Ensuring food security for the growing global population will require increased agricultural output and addressing challenges like water scarcity, climate change, and land degradation. International organizations monitor food security indicators and work to promote initiatives and policies to achieve food security.
Photobacterium damselae subsp. damselae is a marine bacterium that causes infections and disease in many marine animals and can also infect humans. It is a facultative anaerobic, Gram-negative rod that is weakly motile. While it commonly inhabits aquatic environments, it is also a primary pathogen of various fish species that can cause hemorrhaging, septicemia, and ulcerative lesions. In humans, it typically causes opportunistic infections through wounds exposed to salt or brackish water. Its virulence factors include cytotoxins like phospholipase D and pore-forming toxins that contribute to its pathogenicity.
The document provides definitions and examples to distinguish between abbreviations and acronyms. An abbreviation is a shortened form of a name or term, such as "postop" for "postoperative". An acronym is an abbreviation formed from the initial letters of a term, such as "FBI" for "Federal Bureau of Investigation". All acronyms are abbreviations, but not all abbreviations are acronyms. Historically, acronyms formed pronounceable words, but recently acronyms have been formed without consideration of pronunciation. The document then provides guidelines for formatting a list of abbreviations in a document.
Systemic pathology - respiratory, musculoskeletal systems Of FishJuliet Abisha
The document summarizes the musculoskeletal system of fish. It describes the axial skeleton including the skull, vertebrae, and fins. It discusses the appendicular skeleton. It then explains the arrangement of muscle blocks (myomeres) on either side of the axial skeleton which generate propulsive forces. It details the types of muscles including red aerobic muscles and white anaerobic muscles. It also discusses the skeletal tissues including bone composition and cartilage in cartilaginous fish.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
2. Infectious haematopoietic necrosis (IHN) is a viral
disease affecting most species of salmonid fish reared
in fresh water or sea water.
First recognized in 1950s in sockeye salmon and chinook
salmon
3. Caused by the rhabdovirus
the principal clinical and economic consequences of IHN
occur on farms rearing rainbow trout where acute
outbreaks can result in very high mortality.
However, both Pacific and Atlantic salmon can be
severely affected.
4. Aetiological agent
Agent strains
The fish rhabdovirus, IHNV, has a bullet-shaped virion containing
a non-segmented,
negative-sense,
single-stranded RNA genome
5. single-stranded RNA genome of approximately 11,000
nucleotides that encodes six proteins in the following order
a nucleoprotein (N),
a phosphoprotein (P),
a matrix protein (M),
a glycoprotein (G),
a non-virion protein (NV),
a polymerase (L).
6. The causative agent of IHN is a Rhabdovirus , genus
Novirhabdovirus 3 main genotypes described ( Kurath
et al., 2003)
GROUPS ORIGIN
U Isolates from Alaska, British Columbia
Washington,Oregon, California and Japan
obtained from :
Sockeye salmon ( O. nerka )
Chinook salmon ( O. tshawytscha)
M: isolates from Idaho, Washington, France and Italy
obtained from rainbow trout (O. mikiss )
L: isolates from California, Oregon and Japan obtained
from Giappone, obtained from
7.
8. Survival outside the host
IHNV is heat, acid and ether labile.
The virus will survive in fresh water for at least 1 month at
cooler temperatures, especially if organic material is present.
Stability of the agent (effective inactivation methods)
IHNV is readily inactivated by common disinfectants and
drying
9. Life cycle
Reservoirs of IHNV are
clinically infected fish and
covert carriers among
cultured, feral or wild fish.
Virus is shed via urine,
sexual fluids and from
external mucus, whereas
kidney, spleen and other
internal organs are the sites
in which virus is most
abundant during the course
of overt infection
11. Susceptible stages of the host
IHN occurs among several species of salmonids with fry being
the most highly susceptible stage.
Older fish are typically more
resistant to clinical disease,
but among individuals, there
is a high degree of variation
in susceptibility to IHNV.
Survivors of IHN demonstrate a
strong protective immunity with
the synthesis of circulating
antibodies to the virus
12. Target organs and infected tissue
Virus entry is thought to occur through
the gills and
at bases of fins
while kidney, spleen and other internal organs are the
sites in which virus is most abundant during the course of
overt infection.
Salmon affected by IHN virus, ventral congestion and pale gill
13. Disease pattern
Infection with IHNV often leads to mortality due to the
impairment of osmotic balance, and occurs within a
clinical context of oedema and haemorrhage.
Virus multiplication in endothelial cells of blood
capillaries, haematopoietic tissues, and cells of the
kidney underlies the clinical signs.
14. Transmission mechanisms
The transmission of IHNV between fish is primarily
horizontal and high levels of virus are shed from infected
juvenile fish, however, cases of vertical or egg-associated
transmission have been recorded.
15. Although egg-associated
transmission is
significantly reduced by
the now common practice
of surface disinfection of
eggs with an iodophor
solution, it is the only
mechanism accounting for
the appearance of IHN in
new geographical
locations among alevins
16. • CANADA
• USA
• DOMINICAN REP.
• JAPAN
• KOREA
• PAKISTAN
• EUROPE
• BELGIUM
• CZECH REPUBLIC
• GERMANY
• ITALY
• FRANCE
• NETHERLANDS
• POLAND
• SLOVENIA
GEOGRAPHICAL
DISTRIBUTION
officially reported from China, Iran,
Japan and the Republic of Korea.
17. Mortality and morbidity
Depending on the
species of fish,
rearing conditions,
temperature, and, to some extent,
the virus strain, outbreaks of IHN
may range from explosive to chronic.
Losses in acute outbreaks will exceed several per cent of the
population per day and cumulative mortality may reach 90–
95% or more .
In chronic cases, losses are protracted and fish in various
stages of disease can be observed in the pond.
IHN mortality in market size Atlantic salmon
18. TRASMISSION AND PATHOGENESIS
VIRUS ENTRY : Gills , skin, oral
VIRUS SHEDDING : Feces , urine , sessual fluids, mucus
TRANSMISSION : Mostly orizontally
Vertical suspected
Confirmed by vectors ( invertebrates)
TEMPERATURE : Most of the outbreaks at 8-15°C
REPLICATION : Viremia AT 5-10 days
TARGET ORGANS : haematopoietic tissues ( kidney, spleen ) ,
brain and gastro- intestinal.
MORBIDITY & MORTALITY : 90-95% in fry . Not significant in
market-size fish
BTSF
19. Best organs or tissues
The optimal tissue material to be examined is spleen,
anterior kidney, and either heart or encephalon.
In some cases, ovarian fluid and milt must be examined.
Infectious hematopoietic necrosis ( IHN )
in chinook salmon
Collecting-sperm
20. If a sample consists of whole fish with a body length between
4 cm and 6 cm, the viscera including kidney should be
collected.
If a sample consisted of whole fish less than 4 cm long,
these should be minced with sterile scissors or a scalpel,
after removal of the body behind the gut opening.
21. If a sample consisted of whole fish more than 6 cm
long, tissue specimens should be collected as
described above. The tissue specimens should be
minced with sterile scissors or a scalpel,
homogenised and suspended in transport medium.
22. Samples/tissues that are not suitable
IHNV is very sensitive to degradation, therefore
sampling tissues with high enzymatic activities or
large numbers of contaminating bacteria such as the
intestine or skin should be avoided when possible.
Muscle tissue is also less useful as it typically contains
a lower virus load.
23. Field diagnostic methods
Clinical signs
The disease is typically characterised by gross signs
that include
lethargy interspersed with bouts of frenzied,
abnormal activity,
darkening of the skin,
pale gills,
ascites,
distended abdomen,
exophthalmia, and
petechial haemorrhages internally and externally.
photo of affected fry with ascites
24. Black body Color with
exophthalmos(a),
Abdominal enlargement (b),
Pale and weak gill filaments.
There were bleeding in
cephalosome(c), Actinost (d),
abdominal(e)
And the muscular tissue of the
dorsal fin (f).
The liver(g),
Clinical symptoms Of rainbow trout infected with IHNV
Spleen and kidney were pale,
the stomach was swollen with a milky white liquid,
And the bowel released a yellowish liquid.
There were no clinical symptoms in rainbow trout of control group(−).
25. Salmon affected by IHN virus exhibiting peritoneal
and caecal fat haemorrhage
26. Rainbow trout fry -darkening and
exopthalmia (popeye) as shown by the
fish in the lower portion of the photo.
Signs of IHNV disease in rainbow trout (Oncorhynchus mykiss)
include hemorrhage and
exophthalmia (pop-eye)
(photograph at left),
skin darkening relative to
lighter colored healthy fish
(photograph at right)
27. Chinook salmon fry with
infectious haematopoietic
necrosis.
Note characteristic darkening
from the tail region, swollen
stomach and haemorrhaging at
base of fins
Rainbow trout fry with (left)
and without (right)
infectious haematopoietic
necrosis.
Note the darker colour of
the infected fish
29. Behavioural changes
During outbreaks, fish are
typically lethargic with bouts of frenzied,
abnormal activity,
such as spiral swimming and flashing.
A trailing faecal cast is observed in some species.
Spinal deformities are present among some of the surviving
fish.
Rainbow_trout_fingerling
30. Clinical methods
Gross pathology
Affected fish exhibit
darkening of the skin,
pale gills,
ascites,
distended abdomen,
exophthalmia, and
petechial haemorrhages internally and externally.
Internally, fish appear anaemic and lack food in the gut.
The liver, kidney and spleen are pale.
Ascitic fluid is present and petechiae are observed in the
organs of the body cavity.
31. Larger, more robust individuals die first.
Fry are lethargic (swim feebly and avoid current by moving to
the edge of the raceway) with sporadic hyperactivity.
A long, thick, off - white fecal pseudocast trailing from the
rectum is diagnostic.
32. Other clinical signs include darkening, abdominal distension,
exophthalmos, and hemorrhage at the base of the
fins.
Gills are pale, and internally, there is visceral pallor, caused
by anemia.
There is no food in the gastrointestinal tract, which is
distended with an off - white, translucent, mucoid, fluid.
There may be petechiation of the visceral fat, mesenteries,
peritoneum, swim bladder, meninges, and pericardium.
33. In sockeye salmon, 5% or more
of surviving fish may have
spinal deformities.
Clinical signs are less severe in
older fish and may be absent or
simply appear as lateral
compression because of
anorexia .
34. Microscopic pathology
Histopathological findings reveal degenerative necrosis
in haematopoietic tissues, kidney, spleen, liver,
pancreas, and digestive tract.
Necrosis of eosinophilic granular cells in the
intestinal wall is pathognomonic of IHNV infection
35. CLINICAL PATHOLOGY
IHN causes profound changes in cellular and chemical blood
constituents, primarily because of renal damage.
The most diagnostic change is the presence of remnants
of necrotic cells (“ necrobiotic bodies ” ), probably
erythrocytes, in kidney smears.
These cells are less frequent in peripheral blood.
Fish are anemic and leukopenic, and there is evidence of
osmotic imbalance (hypoosmolality).
36. 7-8 months old sockeye salmon naturally infected with IHN.
Fig 1-Kidney imprint. Note necrobiotic body (arrow).
Fig. 2. Kidney imprint. Note necrobiotic bodies (arrows).
37. HISTOPATHOLOGY
In affected fry, major changes are necrosis of
the kidney,
hematopoetic tissue,
pancreas,
gastrointestinal tract, and
interrenal tissue (adrenal cortex).
.
Splenic and renal
hematopoetic
tissues are usually
affected first and
most severely
38. Fig. 3. Kidney section, showing area of focal degeneration
and necrosis (arrow).
Fig. 4. Spleen section, showing area of focal necrosis
involving few cells (arrows)
39. Interrenal tissue may eventually
be involved, as well as glomeruli
and tubules.
Pancreatic necrosis is
common.
Pleiomorphic
intracytoplasmic and
intranuclear inclusions are
present in the pancreatic
acinar and islet cells.
Hepatic necrosis has been reported in some cases.
Necrosis of the eosinophilic granule cells of the intestinal
submucosa (Fig) is highly diagnostic but is only evident in fish at
least 3 – 4 months old
40. In older fingerlings, lesions are similar (splenic and renal
hematopoetic necrosis, moderate sloughing of intestinal
mucosa, degeneration of pancreas) but more subtle.
One distinguishing feature may be the presence of gill lesions
(branchial hyperplasia and fusion)
Peripheral blood smear.
Note large cell in the center,
probably a monocyte, showing
foamy cytoplasm.
10-13 month old sockeye salmon
experimentally infected with IHN
41. 10-13 month old sockeye salmon experimentally infected with IHN.
Fig. 7. Spleen section. Note some degeneration and necrosis and the
lack of lymphoid cells.
Fig. 8. Intestine just posterior to the caeca. Sloughing of the epithelial
layer of the intestine is clearly evident.
42. Histopathology should be supported by at least confirmation
with immunological or molecular probes, when possible.
Virus - infected cells can be immunologically identified in
histological sections or tissue smears from target organs or
blood
Kidney section.
Note the hyaline droplets in
the epithelium of tubules
(arrows).
43. (A) Arrows showed that the
liver cells hemorrhaged
and necrosed, the cell
nucleus was prominent, the
chromatin was condensed,
and the liver cytoplasm
exhibited extensive vacuole
formation.
Histological analysis of rainbow trout experimentally
infected with IHNV
44.
45. (C) Kidney tissue hemorrhaged and necrosed extensively
with characteristics similar to those of the liver.
Renal tubular necrosis led to red blood cells and cell debris
appearing in the lumen.
The interspace was blocked between the necrotic epithelial
cells of the glomerulus and the renal corpusculum
46. (E) The myocardium showed
bleeding, and there were
erythrocytes between muscle
fibers
47. (G) Some back muscle fibers were broken, and the fiber
streak dis-appeared. (H) The muscle fibers showed
bleeding, and red blood cell clusters were visible. (I)
normal muscles
49. show
immunohistochemical
staining (red) of viral
nucleocapsid antigen in
necrotic lesions of exocrine
pancreas cells,
immunohistochemic
al staining (red) of
viral nucleocapsid
antigen in necrotic
lesions of kidney
hematopoietic tissue
50. immunohistochemical staining (red) of viral nucleocapsid
antigen in necrotic lesions of(d) skin – subepidermally below
the basement membrane, (e) granular layer of pyloric cecae
51. IHNV is a relatively weak immunogen.
However, there is little antigenic variation among various
isolates, making serological identification of the virus
relatively simple.
SUBCLINICAL CARRIERS OF IHNV
Adult carriers are asymptomatic.
In female carriers the most sensitive tissues for virus
isolation are ovarian fluid, gills, pyloric ceca, and
kidney.
Postspawning examination of a carrier’s ovarian fluid
is best, since no virus may be detectable for as little as 2
weeks before spawning.
52. Diagnosis
Wet mounts
Wet mounts have limited
diagnostic value.
Tissue imprints and smears
Necrobiotic bodies and foamy
macrophages, indicative of a
clinical manifestation of IHN,
can be best observed using
tissue imprints obtained from
the kidney and spleen rather
than smears.
53. Electron microscopy/cytopathology
Electron microscopy of virus-infected cells reveals bullet-
shaped virions of approximately 150–190 nm in length and
65–75 nm in width.
The virions are visible at the
cell surface or within vacuoles
or intracellular spaces after
budding through cellular
membranes.
The virion possesses an outer
envelope containing host
lipids and the viral
glycoprotein spikes that react
with immunogold staining to
decorate the virion surface.
Electron micrograph of IHN virus
(the rod-like particles)
54. Identification of IHN viruses under transmission
electron microscopy (TEM).
After inoculation with viruses, the EPC cells were craped off.
The virions were cut into slices and observed under TEM.
The virions of rIHNV-EGFP were showed in
A (scale bar 2000 nm), B(scale bar 500 nm)
59. DIAGNOSIS
The suspicion of IHN may be confirmed by virus
isolation and identification of the causative
agent
Select at least 10 symptomatic specimen and
test virologically according to the following
methods :
virus isolation on
epc/bf-2 or fhm/rtg-2
followed by identification
virus isolation and
serological identification
by ifat or elisa or
pcr
60. PREVENTION AND CONTROL
In addition to the disinfection of eggs , the control of IHN
may be obtained by
ERADICATION METHODS
– harvest and eliminate all
the fish population
– dry all the basins simultaneously
(6 weeks)
– disinfect
– restoke with free fish
VACCINATION
– a dna vaccine has been registerd in canada to be used
in salmon industry ( salmo salar) .
BTSF
Editor's Notes
Horizontal transmission of IHNV is typically by direct exposure, but invertebrate vectors have been proposed to play a role in some cases
Examples of clinical signs observed in IHNV-infected rainbow trout.