This document discusses major diseases affecting Asian seabass aquaculture. It describes several viral pathogens like infectious spleen and kidney necrosis disease caused by megalocytivirus, red sea bream iridovirus, and viral nervous necrosis. It also discusses bacterial diseases caused by pathogens like Tenacibaculum maritimum, pathogenic vibrios, Photobacterium damsela, Mycobacterium spp., and Streptococcus iniae. Control measures discussed include biosecurity, general management improvements, vaccination, and antibiotics when administered via feed.
cultured shrimp are getting affected by various disease.some of them are acute and some chronic. and the curing is very harder for a farmer so it is better suggested for safety precaution and proper hygiene while culturing.and the affected shrimp in cured with antibiotics is not accepted by anyone in the export business. so, let yourself find out the various shrimp disease their cure and proper management in this seminar.
cultured shrimp are getting affected by various disease.some of them are acute and some chronic. and the curing is very harder for a farmer so it is better suggested for safety precaution and proper hygiene while culturing.and the affected shrimp in cured with antibiotics is not accepted by anyone in the export business. so, let yourself find out the various shrimp disease their cure and proper management in this seminar.
The presentation on disease management in aquaculture is prepared for academic purpose. Farmers and entrepreneurs make sure that the proposed drug is permissible in their country. Make sure that you contact experts before you implement them.
The measures and methods adopted to secure a disease free environment in all phases of aquaculture practices (i.e. hatcheries, nurseries, grow-out farms) for improved profitability.
Any aquatic invertebrate animals having a cutaneous or calcareous shell surrounding there body and belonging to the phylum Mollusca, the class Crustacea (phylum Arthropoda), or phylum Echinodermata is known as shellfish. The term is often used for the edible species of the groups, especially those that are fished or raised commercially. The most commercially important shellfish are:
• Mollusk: Oysters, mussels, scallops and clams
• Crustacean: Shrimp, prawn, lobster, crab and crayfish
• Echinoderm: sea urchins and sea cucumbers
Shellfish hatchery is a place where shellfish seeds are produced in a controlled way. Hatchery management is a branch of science which deals with the activities including from collection of brood shellfish to seed production. Culturing of shellfish has occurred since ancient times. Although controlled rearing of young shell has long existed, hatchery production is a more recent advancement. Producing seed under controlled conditions in a hatchery will disconnect its production from environmental factors and provide a reliable supply of seed. Oysters, mussels and mud crabs are the most important groups of shellfish after shrimp and prawn. These are popular among the western countries and becoming more popular all over the world. So hatchery management of oyster, mussel and crab is crucial.
Aquaculture is the most reliable sector to the providing world nutrias food. It is all depended on the cultivable species. Asian seabass is one of the candidate species for aquaculture because of the wide range of water quality tolerance, growth performance, and consumer preference. The Indian and Western Pacific Oceans are where Asian seabass is found in its natural habitat. It can be cultured in the earthen pond, floating or stationer cages, and recirculating system. Seed resources are available in rivers and lakes of fresh water, but aquaculture is the depending on the hatchery that produces seed because of the superior growth production. In the nursery phase, cannibalism is predominant in Asian seabass cultivation, which will improve via the grading of a shooter. Feeding is one of the important management for good growth performance and reducing cannibalism to give the optimum feed requirements. The growth rate of the Asian seabass is 400-600 g in 4 to 6 months. Asian seabass farmers are suffering from some infectious and non-infection diseases, it will improve via the good management practices of the culture promises. In this review paper, some key points of Asian seabass farming are covered for better understanding.
SYSTEMATIC POSITION of clams
What is the difference between mussels, oysters, scallops and clams?
General characteristics of clams
Distribution and habitat of clams
BIOLOGY of clams
Breeding habit of clams
Present status of production
Craft and gear
Clams have two symmetrical shells(Bivalve)
They can filters their food
Clams can control their outer shells and shut them in response to stimuli, via a elastic ligament and two large muscles
In side their usually grey, black shells you can see a white, tan center
Clams have siphons that forces water out and allows them to take in micro organisms
There are over 150 edible species.There are over 15,000 species of clams.Small freshwater clams fertilize eggs in a pouch and bear their young until its shell develops.
The Giant clam can weigh more than 400lb and live for over 150 years.
It takes 3-4 years for a clam to mature to market size.
Some clams can produce pearls.One in 5,000 clams forms a pearl.
A clam can live until about 35 years if not eaten.
distribution:
Marine clams are abundant in the low and mid intertidal zone in temperate seas globally. Other species of marine mussel live in tropical intertidal areas, but not in the same huge numbers as in temperate zones.
Certain species of marine clams prefer salt marshes or quiet bays, while others thrive in pounding surf, completely covering wave-washed rocks. Some species have colonized abyssal depths near hydrothermal vents. The South African white mussel exceptionally doesn't bind itself to rocks but burrows into sandy beaches extending two tubes above the sand surface for ingestion of food and water and exhausting wastes.
Freshwater clams inhabit permanent lakes, rivers, canals and streams throughout the world except in the polar regions. They require a constant source of cool, clean water. They prefer water with a substantial mineral content, using calcium carbonate to build their shells.
Fusarium infects dead or damaged tissue caused by wounds or other infections resulting in locomotory difficulties due to mycelial growth. High mortality in susceptible populations. Fouling of the gills by these organisms probably results from poor husbandry.
The presentation on disease management in aquaculture is prepared for academic purpose. Farmers and entrepreneurs make sure that the proposed drug is permissible in their country. Make sure that you contact experts before you implement them.
The measures and methods adopted to secure a disease free environment in all phases of aquaculture practices (i.e. hatcheries, nurseries, grow-out farms) for improved profitability.
Any aquatic invertebrate animals having a cutaneous or calcareous shell surrounding there body and belonging to the phylum Mollusca, the class Crustacea (phylum Arthropoda), or phylum Echinodermata is known as shellfish. The term is often used for the edible species of the groups, especially those that are fished or raised commercially. The most commercially important shellfish are:
• Mollusk: Oysters, mussels, scallops and clams
• Crustacean: Shrimp, prawn, lobster, crab and crayfish
• Echinoderm: sea urchins and sea cucumbers
Shellfish hatchery is a place where shellfish seeds are produced in a controlled way. Hatchery management is a branch of science which deals with the activities including from collection of brood shellfish to seed production. Culturing of shellfish has occurred since ancient times. Although controlled rearing of young shell has long existed, hatchery production is a more recent advancement. Producing seed under controlled conditions in a hatchery will disconnect its production from environmental factors and provide a reliable supply of seed. Oysters, mussels and mud crabs are the most important groups of shellfish after shrimp and prawn. These are popular among the western countries and becoming more popular all over the world. So hatchery management of oyster, mussel and crab is crucial.
Aquaculture is the most reliable sector to the providing world nutrias food. It is all depended on the cultivable species. Asian seabass is one of the candidate species for aquaculture because of the wide range of water quality tolerance, growth performance, and consumer preference. The Indian and Western Pacific Oceans are where Asian seabass is found in its natural habitat. It can be cultured in the earthen pond, floating or stationer cages, and recirculating system. Seed resources are available in rivers and lakes of fresh water, but aquaculture is the depending on the hatchery that produces seed because of the superior growth production. In the nursery phase, cannibalism is predominant in Asian seabass cultivation, which will improve via the grading of a shooter. Feeding is one of the important management for good growth performance and reducing cannibalism to give the optimum feed requirements. The growth rate of the Asian seabass is 400-600 g in 4 to 6 months. Asian seabass farmers are suffering from some infectious and non-infection diseases, it will improve via the good management practices of the culture promises. In this review paper, some key points of Asian seabass farming are covered for better understanding.
SYSTEMATIC POSITION of clams
What is the difference between mussels, oysters, scallops and clams?
General characteristics of clams
Distribution and habitat of clams
BIOLOGY of clams
Breeding habit of clams
Present status of production
Craft and gear
Clams have two symmetrical shells(Bivalve)
They can filters their food
Clams can control their outer shells and shut them in response to stimuli, via a elastic ligament and two large muscles
In side their usually grey, black shells you can see a white, tan center
Clams have siphons that forces water out and allows them to take in micro organisms
There are over 150 edible species.There are over 15,000 species of clams.Small freshwater clams fertilize eggs in a pouch and bear their young until its shell develops.
The Giant clam can weigh more than 400lb and live for over 150 years.
It takes 3-4 years for a clam to mature to market size.
Some clams can produce pearls.One in 5,000 clams forms a pearl.
A clam can live until about 35 years if not eaten.
distribution:
Marine clams are abundant in the low and mid intertidal zone in temperate seas globally. Other species of marine mussel live in tropical intertidal areas, but not in the same huge numbers as in temperate zones.
Certain species of marine clams prefer salt marshes or quiet bays, while others thrive in pounding surf, completely covering wave-washed rocks. Some species have colonized abyssal depths near hydrothermal vents. The South African white mussel exceptionally doesn't bind itself to rocks but burrows into sandy beaches extending two tubes above the sand surface for ingestion of food and water and exhausting wastes.
Freshwater clams inhabit permanent lakes, rivers, canals and streams throughout the world except in the polar regions. They require a constant source of cool, clean water. They prefer water with a substantial mineral content, using calcium carbonate to build their shells.
Fusarium infects dead or damaged tissue caused by wounds or other infections resulting in locomotory difficulties due to mycelial growth. High mortality in susceptible populations. Fouling of the gills by these organisms probably results from poor husbandry.
Infectious pancreatic necrosis (IPN) is a severe viral disease of salmonid fish. It is caused by infectious pancreatic necrosis virus. It make huge economic loss on aquaculture industry every year.
White spot syndrome virus (WSSV)White spot syndrome (WSS) is a viral infection of shrimp. Outbreaks of this disease have wiped out the entire populations of many shrimp farms within a few days, in places throughout the world.
aquaculture shrimp farming diseases.
Presentation 8: Vibrio parahaemolyticus: a versatile pathogen that can adapt ...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Successstory of a Gujarat Inland wetland farmer by Bhaskar.pdfB. BHASKAR
Inland open water wetlands are Potential source for culture based capture fisheries, a success story of small scale wetland farmer of Gujarat, Inland Fishes and prawns
Major Ornamental fish Exporters contact details in India by BB. BHASKAR
Major Ornamental fishes and plants exporters registered contact details in East and West coast states of India. mission Ornamental fisheries,
List of state wise Public aquariums, Indigenous Fresh water and Marine water ornamental fish aquarium, and ornaments brood bank capital and operational cost for set up and for promoting ornamental fish farming for small scale livelihoods and entrepreneurs
IMPORTANCE OF WATER IN SUPPORTING LIFE ON THE PLANET By B.pptxB. BHASKAR
2024 World water day theme: Water for peace.
World water resources, its percentage share in human & other animals body, health maintenance, agricultural production sector for achieving food security, industrial sector, poverty alleviation. World prospects on water use efficiency & saving water quality for drinking, food production & other applications through responsible uses
Threatned Sharks Rays and Chaimera along Arabian sea By B.pptxB. BHASKAR
Introduction about sharks, Rays and Chaimeras, Fishery exploitation status along Arabian Sea waters, present threatened families of sharks, Rays and Chaimeras
Fish stem cells and their applications by BB. BHASKAR
Different types of stem cells in humans and Fishes, importance and Application of fish stem cells in Aquaculture, conservation and fish health healing mechanism, UV-rays protection and other intigrated uses of stem cells for industrial purpose, fish cell culture
Fishes unique care wishes on occasion of valentine's day wishesB. BHASKAR
Loving beautiful fishes also caring youngones. Male fishes Show their love in care by bearing female fishes parental care risk.
male fishes only pairing with single females fishes throughout its lifespan and guards their eggs by incubation during birth till hatching out
Lobster breeding seedproduction and rearing and fattening in CagesB. BHASKAR
Commercially important exploited Lobster species of India, Breeding, seed production, larval rearing and nursery rearing, pit culture and open sea cage culture of lobsters, cost economic of lobster Aquaculture, water quality & feeding management, SWOT Analysis in experimental lobster farming. Constraints and Recommendations
Continents of the world and FAO fishing areas, Ocean fisheries and coastal countries, large seas, marine capture fisheries production of the world major fishing areas, generals, and species.
Past to present fish Seed production in India ln states, UTs and Indian major carps brood stock maintainace, breeding, maturation stages, embryonic developmental stages, fish spawn, Fry, Quality checking, Quarantine principle for seed quality.
Dams Impacts on environment with special focus on Wild Fisheries.pptxB. BHASKAR
Major Indian Rivers and their Dams
Species diversity reported along streches of Rivers, Dams impacts on capture fisheries due to habitat alterations, world scenario of Dams impacts
Fish Products Exporters registered under MPEDA in India by B.pdfB. BHASKAR
Address and contact details of Fish and Fishery products Exporters from different states and UT's registered under Marine products Export development Authority (MPEDA)of India, helpful for interested Aqua farmers, entrepreneurs interested for export of seafood and professionals awareness
Introduction to Riverine systems in India, Origin hills, East and West coast riverine systems, tributaries of major rivers, length, catchment areas and Ichthyo-faunal diversity and major fisheries of riverine systems
Efficient utilization of Fish waste.pptxB. BHASKAR
Introduction to fish waste, % of fish body parts and their by products potential valuable applications. Utilization of Shell fishes waste, smart sensors in detection, AI application in valorize of big data fish reducing fish waste
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
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Seabass Diseases and control measures by B.pptx
1. Major reported diseases in Asian Sea bass
and recently known control measures
practiced in the world view
By:
B. Bhaskar
Dedicated to Aqua
farmers for preventing
losses by controlling
diseases
2. Introduction
• Seabass is considered as a euryhaline and catadromous species, it inhabits in
freshwater, brackish and marine habitats including streams, lakes, estuaries and,
coastal waters. Adults migrate to the sea for gonadal maturation and subsequent
spawning. It is distributed throughout the Indo-Paci c region.
• The countries that are popular for culture of seabass are Australia, Taiwan,Thailand,
Singapore, Indonesia, Philippines, and Malaysia. In Australia, seabass is locally
called as Barramundi and considered as an important food fish.
• It can be farmed in ponds and cages from freshwater to seawater environments. The
fish can grow to 1.0 kg size in 6-8 months by consuming pellet feed. Availability of
hatchery produced seed and formulated pellet feed ensures the year-round supply
and thus enables easy adoption of seabass farming by farmers. It can also be farmed
in net cages, floating cages and Recirculatory Aquaculture System (RAS).
• Seabass is carnivorous in nature. In the wild, they consume zooplanktons in their
very early stages and when they grow to 10-20 cm length; they prey upon small
fishes and shrimps. However, when this fish is farmed in the ponds and cages, it can
be adapted to consume formulated feed.
• Rotifer, Brachionus plicatilis can be supplied as first feed to seabass larvae from
2nd day (dph) and continued up to 15th dph. Meanwhile, Artemia nauplii can also
be included as feed from 9th dph along with rotifer. From 15 to 30 dph, Artemia
nauplii can be supplied along with artificial feed from 17 dph. The fry can be
completely weaned from the artificial diet between 30-35th dph.
4. Infectious spleen and kidney necrosis disease (ISKND):
• Causative agent: Infectious spleen and kidney necrosis virus (ISKNV) or
called Megalocytivirus ISKNV • Enveloped icosahedral viruses (130-150 nm in
diameter) with a linear double-stranded DNA genome of 111 kb.
• Clinical signs: darkening, pale gills, ascites, enlarged spleen (splenomegaly).
• Mortality: High mortality up to 85% of Asian sea bas.
• Susceptible stage: fingerling, juveniles, and grow-out of Asia sea bas.
• Transmission: horizontal & vertical.
• Distribution: Widely distributed • Cases in Asian sea bass = Vietnam, Thailand,
China.
• Histopathology: Basophilic hypertrophied cells (megalocytes) in spleen, kidney,
liver, and gills.
• Diagnosis: PCRs, qPCR, isothermal amplification, immunological based techniques.
• Cell culture: Grunt fin-1 (GF-1) =Vacuolization Mandarin fish fry cells (MFF-1) =>
Rounding cells & detachment
• Genotyping: MCP gene
• Prevention: Biosecurity + general managements
5. Red sea breamiridovirus(RSIV)
• 80-90% mortality of grow-out open-caged Asian sea bass in the
brackish water environment in India.
• RSIV positive using DNA polymerase PCR and sequence analysis.
• more closely related to Korean isolate of RSIV.
• examinations were investigated.
• reproduced 100% mortality in the healthy sea bass.
• Fig:Infected Asian seabass showing Pale gills and enlarged spleen
6. Turbot reddish body iridovirus(TRBIV):
Tsai et al, 2020. Viruses 12: 681–699.
• L. calcarifer imported to Taiwan die
up to 90%.
• body color, petechiae of gills,
severe anemia and an enlarged
abdomen.
• well as reddish livers.
• infection of TRBIV genotype II.
• -nested PCR was developed to
improve detection sensitivity.
7. SDDV (Scale drop disease virus)
• SDDV (Scale drop disease virus) farmers also called red belly disease
• => Scale drop disease
• oLCHV (Lates calcarifer herpesvirus)
• =>SDD-like symptom
• oA unique strain of Vibrio harveyi
• => scale drop and muscle necrosis
• Causative agent:Scale drop disease virus (SDDV)
• •Enveloped hexagonal virions (100-180 nm) with double-stranded DNA genome of 131 kb (135 ORFs). & SDDVin
freshwatercultured Asian sea bass co-infected with Flavobacterium
• clinical signs: Asian sea bass: Scale loss, some with “red belly”
• Mortality: Asian sea bass: 40-50% in marine, brackish, and freshwater culture. susceptible stage- Juveniles, subadut, adult.
• Histopathology: Basophilic hypertrophied cells (megalocytes)
• Multifocal necrosis, pyknosis and karyorrhexis
• Dermal inflammation and severe infiltration of lymphocytic inflammatory cells
• Diagnosis: PCRs,qPCR, isothermal amplification
• Genotyping: Major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes .
• Cell culture: Seabass kidney (SK) SK21 cells Grunt fin (GF-1) cells MFF-1 cell.
• Prevention: Biosecurity + general managements
• Ref: Gibson-Kuehet al. 2012 J Fish Dis 35:19-27 de Groof et al. 2015 PlosPathog11(8): e1005074
8. LCHV, Lates calcarifer herpesvirus
• Diseased fish with SDD-like symptom
• Singapore and Vietnam, 2015
• Mortality 30-70% (natural)
• 77% mortality in co-habitation or i.p.challenges
• Genome 130 kb
9. Scale Drop and Muscle Necrosis Disease (SDMND)
• Dong et al. Aquaculture (2017) 473:89-96
10. Viral Nervous Necrosis (VNN)
or Viral encephalopathy and retinopathy (VER) disease
• Causative agent: Betanodavirus, Nervous necrosis virus (NNV)
• Non-enveloped icosahedral virus (appx 25 nm in diameter) with two positive-strand
RNA segmentsof 3.1 and 1.4 kb
• Transmission: Horizontal & vertical
• Clinical signs: Darkening of the skin & erratic swimming. Some with pop-eye. Viral
Nervous Necrosis (VNN) is acute in larvae that are ten days old and oftenresults in 100%
mortality. The transparent larvae (due to the contraction ofchromatophores) will
display corkscrew or whirling swimming patterns and havehyper-inflated swim
bladders.
• Mortality:upto 100% in Marine water host fishes & Susceptible all stages, mainly larvae
and juveniles .
• Diagnosis: RT-PCRs,qPCR, isothermal amplification, Immunological based techniques
• Histopathology: Vacuolation in brainandretina
• Genotyping: RNA 2 segment encoding a viral capsid protein
• Cell culture: Striped snakehead cell line, SSN-1E-11, a clone of SSN-1 & others
• Prevention: Biosecurity + improvement of host immunity + water management +
reduce stress
• Ref: Ziaratiet al. Current Microbiology (2020) 77:3919–3926
12. Red eye', an often-typical clinical sign of Iridovirus in
barramundijuveniles because of massive internal
haemorrhaging
• In barramundi the disease mainly occurs in fish of 10 to50g and
causes acute peaks of mortality of up to 80 - 90% (Picture 5). The
fish willturn black and lose appetite. On closer clinical examination
the gills will appear verypale and may bleed when handled and
blood may also leak into the iris giving a ‘redeye' appearance (
Picture 6). Internally, a pale spleen is characteristic of this disease.
13. Bacterial diseases in Asian seabass
• Bacteria Tenacibaculum maritimum (T. mar)Tenacibaculum maritimum, also described as T. mar, is a rod shaped
gram negative,filamentous bacterium. In Singapore, the team has been working with this pathogensince 2003.
There are 95 isolates from China, Indonesia, Malaysia,Singapore, Korea, Japan and Greece stored in the
laboratory.
• T. mar can be especially severe when combined with skin parasites and outbreaks ofthe disease often occur after
a stressful event. Typically, a T. mar outbreak will startas small lesions on gills and the ventral side of the fish,
resulting in chronic scale lossand spreading to other cartilaginous body parts including the face and jaw.
• pathogenic vibrios which have been isolated from seabass include Vibrio parahaemolyticus, V.
anguillarum and V. alginolyticus.
• Pasteurellosis – Photobacterium damsela.
• Gliding bacterial disease/tail rot disease (Flexibacter sp.):Ampicillin and florfenicol have been reported
to be effective when administered in feed. Tenacibaculum maritimum (formerly Flexibacter maritimus
• Mycobacteriosis
• Recent report sindicate that this disease is becoming more severe with devastating losses of nurseryfish after
stocking in pre-grow out cages (Note 2009).
• Late stage progressive ‘saddleback' skin lesions due to
T.maritimum& Isolation of the rod shaped bacterium on selective media illustrating
therusty colouration, Presumptive diagnosis on the farm is possible using a gram stained
wet mount of askin scrape from the edge of an infected lesion.
• coupled with rapid onset and transmission make therapeuticcontrol extremely difficult.
Prevention of the disease through vaccination prior toexposure is the strategy for the near
future.
14. Streptococcus iniae
• The most important bacterial species affecting the culture of
barramundi throughout the tropics is Streptococcus iniae.
Indeed, S. iniae is considered to be one of the most serious
bacterial diseases of all warm water fish.
• Typical clinical signs associated with S. iniae of exophthalmia
and internal septicaemia.
• In barramundi, the disease is systemic and characterised by
massive acute mortality peaks with cumulative mortality of
70% being very common.
• Infections can occur throughout the entire growth cycle but
are more often seen in large and harvest size fish. S. iniae is
therefore considered to be an ‘expensive' disease for farmers
who will experience a loss of production efficiency and
marketable product but more importantly a reduction in
overall feed conversion rates.
15. Norvax® Strep Si, a water-based inactivated vaccine,
registered for use against S. iniae infections in farmed fish
• Since 2005, Intervet/Schering-Plough Animal Health has Norvax® Strep Si,
a water-based inactivated vaccine, registered for use against S. iniae
infections in farmed fish.The vaccine was designed with two applications in
mind; the first of which is as animmersion vaccination in which fish are
immersed for 30 seconds at approximately2g. In the laboratory, protection
from immersion vaccination has been confirmed for 5weeks. The second
application method is by intraperitoneal injection (IP) and isconducted when
fish reach 15g. Laboratory studies indicate that when 15gbarramundi are
vaccinated using a single intraperitoneal (IP) injection with 0.1ml
ofNorvax® Strep Si, maximum protection is achieved as soon as one week
aftervaccinationStudies under field conditions after IPvaccination at 15g
have demonstrated protection up to 18 months post vaccination when fish
are >2kg.
16. Reported few parasitic diseases in Asian seabass
• ParasitesIte is almost impossible to avoid parasites because usually they are present as part
ofthe aquatic ecosystem.
• Helminthes parasites which have been found in seabass include monogenetic trematodes,
digenetic trematodes, nematodes and acanthocephala.
• Crustaceans belonging to the Branchiura, Copepoda, Isopoda and Amphipoda are
frequently found on the body surface and/or gills of caged marine fish.
• Parasitic copepods:Argulus sp.& Caligus sp. has caused big problems in cultured
seabass&Lernanthropus sp. are found attached to the gill of seabass especially in cage
cultured fish.
• Parasitic Isopods: Nerocila sp. and Gnathia sp. have also been reported in seabass.
• Health monitoring and early diagnosis is key for control ofparasitic diseases. Protozoa
(particularly Cryptocaryon irritans and Trichodina spp.)and capsalid monogeans on the
body surface (particularly Neobenedinia spp.) most commonly affect newly stocked
barramundi in open ocean cages. Current observations and reports from South East Asia
indicate that capsalid monogean infections are the most serious and pathogenic amongst all
of the parasitic diseases. If left untreated barramundi quickly develop skin and tail rot and
mortality rates of 30-40% are common. Neobenedinia most commonly affects younger fish
but if a population has been compromised in anyway (even from the nursery stage) then
they will always be susceptible. Typically, a proportion of the fish will be ‘off feed' and
lethargic. The parasite also irritates the eyes causing opacity and exophthalmia (‘pop eye')
& gradually the caudal and pectoral fins will become frayed (white appearance in the
water) and hemorrhagic when handled.
17. Dorsal fin of a barramundi illustrating
opaque Neobenedinia parasites after
immersion in freshwater
• Neobenedinia is particularly easy to spot at the farm because after the
whole fish is immersed in freshwater for some minutes, the parasites
will turn opaque
• A specific parasite prevention program with routine freshwater
immersion, skinscrapes and gill clips should be considered an integral
part of the health management protocols.
18. Rational
• In Addition to diseases and abnormalities due to
environmental contaminants and nutritional
deficiencies have been recognized as important
problems in fish culture whenever diets as well as
control or water quality become inadequate.
Malnourishment or undernourishment of seabass
under culture can result in slow growth,
susceptibility to diseases or death.
• Strategies for aquacultured Asian sea bass.
• No treatment for viral diseases in aquaculture, so
‘Prevention is better than cure’.
• Vaccination and selective breeding are envisioned as
future disease management