1. The study isolated fungi from six freshwater fish species, finding fungal infection in Labeo rohita and Carassius auratus. 2. Aspergillus spp, Mucor sp, and Penicillium spp were isolated from the gills, fins, and skin of C. auratus. Only Aspergillus sp was isolated from the caudal fin of L. rohita. 3. The presence of toxigenic fungi like Aspergillus increases health risks for both humans and animals through potential mycotoxin production.