Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Induced Pluripotent Stem Cell & Cell Dedifferentiation: The Breakthrough of S...Vincentsia Vienna
The phenomenon of cell dedifferentiation is yet one promising trend to explore. In future, the science fiction of regenerative medicine could be turned into reality.
it gives detail or you can say brief introduction of iPS cells , what are they , how can be obtained , what are the future possibilities of iPS cells what promise it made to upcoming future technology to medical health
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Induced Pluripotent Stem Cell & Cell Dedifferentiation: The Breakthrough of S...Vincentsia Vienna
The phenomenon of cell dedifferentiation is yet one promising trend to explore. In future, the science fiction of regenerative medicine could be turned into reality.
it gives detail or you can say brief introduction of iPS cells , what are they , how can be obtained , what are the future possibilities of iPS cells what promise it made to upcoming future technology to medical health
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Stem cells in regenerative biology and medicinePasteur_Tunis
Présentation réalisée par Shahragim Tajbakhsh durant le cours du réseau international des instituts Pasteur de "Médecine Génomique: du diagnostic à la thérapie " (17-21 octobre 2016)
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
Introduction.
Properties of Stem Cells.
Key Research events.
Embryonic Stem Cell.
Stem cell Cultivation.
Stem cells are central to three processes in an organism.
Research & Clinical Application of stem cell.
Research patents.
Conclusion.
Reference.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Stem cells in regenerative biology and medicinePasteur_Tunis
Présentation réalisée par Shahragim Tajbakhsh durant le cours du réseau international des instituts Pasteur de "Médecine Génomique: du diagnostic à la thérapie " (17-21 octobre 2016)
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
Introduction.
Properties of Stem Cells.
Key Research events.
Embryonic Stem Cell.
Stem cell Cultivation.
Stem cells are central to three processes in an organism.
Research & Clinical Application of stem cell.
Research patents.
Conclusion.
Reference.
Stem cells are one of the important cells present in both plant and animals. these cells have ability to regenerate any part of the body work similarily as meristem cells in plant. The advances in the stem cell technology has open a new era in medical field. the advances in this technology has been presented here and their important application has been included in this present in this presentation.
This presentation was developed by Martha Lopez Yrigoyen.
It is intended as a flexible tool for lectures and tutorials for first year students in the Biological and Biomedical Sciences. Diagrams are also encouraged to be used by scientists, science communicators and educators.
Not all slides will be useful for everyone or for every occasion. Slides can be adapted for the purpose needed.
There are two main activities intended for undergraduate students to engage and deepen their knowledge in the field of stem cells. The first activity is more general and its aim is to familiarize students with the core concepts in stem cell and regenerative medicine. The second activity is a novel and current example of how induced pluripotent stem cells are being used in cutting edge biomedical research. The two publications suggested for students to read can be swapped for other examples.
Presentation is licensed under a Creative Commons Attribution License 4.0
https://creativecommons.org/licenses/by/4.0/
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
FIDO Alliance Osaka Seminar: The WebAuthn API and Discoverable Credentials.pdf
Induced Pluripotent Stemcells: A P4 Perspective
1. Induced Pluripotent Stem Cells: The Personal Stem Cell Emile F. Nuwaysir, Ph.D. Chief Operating Officer Cellular Dynamics International
2. Induced Pluripotent Stem Cells Introduce four reprogramming factors Blood draw or skin biopsy Induced pluripotent stem (iPS) Cells iPS cells are not the same as embryonic stem cells, and are NOT derived from fertilized embryos! iPS cells are derived from adult tissue sample (skin or blood)
3. Induced Pluripotent Stem CellsTwo Critical Characteristics What’s so special about iPS cells? Replicate Indefinitely Make all 208 cell types in human body Induced Pluripotent Stem (iPS) Cells
50. Express all function cardiac markers, including critical ion channels
51.
52. iCell Cardiomyocytes In Vitro Human Electrophysiology Cells Display Normal Ionic Currents Cells Display Normal Action Potentials Action Potential Examples Atrial-like Nodal-like Ventricular-like Control Tracings 30nM E4031 Control Drug Responses 3 nM Nifedipine 10 nM Nifedipine Control 100 ms
54. Testimonials “Had stem cell-derived heart tissue been available… the company could have pulled the plug early, saving two years of work and millions of dollars...” - Kyle Kolaja, global head of predictive toxicology screens and emerging technologies for Roche. Sep 23, 2010, BloomBergBusinessWeek, “Stem Cells Test Drugs in a Dish, May Save Companies Millions” Roche "This is a game changer. It's going to dramatically change biology and drug development.” - Sandra Engle, senior principal scientist at Pfizer. Jan 22, 2010, MIT Technology Review “Human heart cells that beat in a dish have never before been available in large quantity and consistent quality. Because they are functional human cells, they may provide a more accurate way of testing drugs than using rodents or the preserved human cells that have been available for lab research in the past,” - Jason Gardner, vice president and head of the stem cell drug performance unit at GlaxoSmithKline. Sep 23, 2010, BloomBergBusinessWeek, “Stem Cells Test Drugs in a Dish, May Save Companies Millions” Pfizer GlaxoSmithKline
59. Express key molecular and functional attributesTypical morphology observed early after differentiation (left). Three weeks later, the neurons are more organized into extensive networks (below). TH/Hoechst Glutamate/Hoechst GABA/Hoechst
71. iCell Hepatocyte CharacterizationFunction Lipid Storage Glycogen Storage Albumin Production 3x induction PAS Oil Red 2x induction 60% of iCell Hepatocytes store lipid (BODIPY assay) CYP3A4 Protein Expression CYP3A4 Activity
82. Provides a promising approach to test GWA results and common DNA variation in the context of a cell-based system by directly assaying molecular function and effects.
Response to cytokine stimulation causesupregulation of adhesion molecules
Points to emphasize: Slide shows a summary of key hepatocyte characteristics that we are looking for in our iPS cell-derived hepatocytesThis image is of CDI’s iCellHepatocytes
Albumin Production Points to emphasize: iPS cell-derived hepatocytes expression levels of albumin protein that are similar to PHH, and much greater than HepG2 cells (top left panel)Albumin was measured via ELISAOther things to know: Albumin is measured and expressed as pg/ALB+ cell/day Number of ALB+ cells was determined from the actual wells that were assayed after 24-48 h of incubation, not from the number of hepatocytes seeded. Glycogen & Lipid Storage Points to emphasize:iPS cell-derived hepatocytes store glycogen, as measured by Period Acid Schiff (PAS) staining (top middle panel)iPS cell-derived hepatocytes store lipid, as measured by Oil Red staining (top right panel) Lipid storage has also been measured using a BODIPY assay, and 60% of the iPS cell-derived hepatocytes store lipid (data not shown)Other things to know: Cells are counter-stained with hemotoxylin (purple nuclei)CYP3A4Points to emphasize: Data show CYP3A4 protein expression and activity Bottom left panel: see characteristic cytoplasmic staining of CYP3A4 (red) vs. nuclear staining with DAPI (blue). Bottom right panel: CYP3A4 activity measured in iCellHepatocytes and HepG2 cells using Promega’s P450-Glo assay (IPA substrate which is 1000x more specific to CYP3A4 vs. CYP3A5 or CYP3A7). This assay (and the P450-Glo assay for CYP1A2) is used routinely at CDI for monitoring P450 activity during development. Once highly pure cells are generated, P450 activity (basal and induced) will be measured by mass spec through external collaborations Cells were induced with 10uM DEX. Other things to know: Data represent the average of 3 lots of iPS cell-derived hepatocytes and 6 lots of HepG2 cells (various passages)iPS cell-derived hepatocytes express CYP3A7 mRNA, but no protein is detected by immunoblot and no activity is detected using Promega’s P450-Glo assay specific for CYP3A7).
