The in vitro interaction between n-hexane extract (nhexEXT) of root of Adiantum capillus-veneris and certain known antimicrobial drugs i.e. Oxacillin, Ceftazimide, Cefriaxone, Ofloxacin, Meropenem, Erythromycin, Cefuroxime, Cefoxitin, Cefotaxime and Ampicillin was evaluated. The study was carried out against ten bacterial strains (Staphylococcus aureus, S.epidermidis, Salmonella typhi, Klebsiella pneumoniae, Shigella dysentriae, Proteus vulgaris, Pseudomonas aeruginosa, Providencia species, Citrobacter freundii and Escherichia coli isolated from urine, pus and blood samples. Both disc diffusion and well diffusion methods were used to determine antimicrobial activity of plant extract in combination with antibiotics. Antimicrobial sensitivity indicated that Meropenem was the most effective antibiotic with zone of inhibition (ZI) of 25-33 mm among all tested antibiotics followed by Ofloxacin (10-26.5 mm), Ceftriaxone (8-20 mm), while Oxacillin showed no activity against almost all bacterial strains. The study showed that most bacterial strains were resistant to most of the antibiotics used, ranging from 20-60%. The methanolic extract (nhexEXT) of A. capillus-veneris used alone was active against most of the bacterial isolates with maximum activity against E. coli with 16 mm ZI. The study also indicated that there was an increased activity in case of combination of nhexEXT with antibiotics. The combined effects of plant extract with antibiotics were synergistic against most of the bacterial strains. The nhexEXT showed maximum synergistic effect with Ceftazimide with ZI of 42 mm followed by Meropenem (40 mm) and Ceftriaxone (28 mm) against multidrug-resistant (MDR) bacterial strains. The data suggests that plant extract could be used as alternative to antibiotics. These results give scientific backing that combination between plant extract and antibiotics would be useful in fighting the emerging drug-resistant bacterial pathogens.
Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimu...ijtsrd
In this study, clinical isolate of Staphylococcus aureus was subjected to susceptibility tests against commercially available antibiotics and Ocimum gratissimum scent leaf leaf extracts. disc diffusion nmethod was adopted for the antibiotics test while agar well diffusion technique was employed for the antimicrobial screening of the leaf extracts. The result shows that the organism was sensitive to Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing 40 of the antibiotics while showing outright resistance to Ampiclox, Amoxicillin and Erythromycin 30 and intermediate results for the rest. Also, the cold water extract of O. gratissimum did not show any activity against the bacterium. However, both hot water extract and ethanol extract of the plant had varying degree of activity against the organism with ethanol extract recording a zone of inhibition of 25.33 mm compared with 32.00 mm recorded for Gentamycin. Furthermore, the minimum inhibitory concentration recorded ofr the extract against the organism were 10 mg ml and 2.5 mg ml respectively for hot water and ethanol extracts. These results indicate that ethanol extract of O. gratissimum leaf is very effective against Staphylococcus aureus and could be used as potential source of natural product for the treatment of infections caused by the organism especially the antibiotic resistant strains. Komolafe T. O. | Ogunyankin O. G "Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46333.pdf Paper URL : https://www.ijtsrd.com/other-scientific-research-area/other/46333/antibiogram-of-staphylococcus-aureus-and-its-sensitivity-to-ocimum-gratissimum-extract/komolafe-t-o
Protein was extracted from muscles of Channa striatus and attempts were
made to evaluate in vitro antibacterial activity against clinical bacterial isolates. The
higher concentration of protein (100μg/ml) extracts exhibited a pronounced activity
against Pseudomonas aeruginosa (21 mm), Proteus vulgaris (19 mm), Citrobacter sp
(19 mm), Klebsiella pneumoniae (18 mm), Micrococcus sp (17 mm), Bacillus subtilis (16
mm), Staphylococcus aureus (15 mm), E. coli (14 mm) and Serratia marcescens (5
mm). The minimum inhibitory concentration and minimum bactericidal concentration
were found to be 20-40 μg/ml and 80-100 μg/ml respectively for the extracts of
Channa striatus protein against test organisms. This study confirms that C. striatus fish
protein extracts possess antibacterial activity against a wide range of microbes and
justified that it could be used in the traditional medicine as a remedy for the
treatment of bacterial diseases.
A study of antibiotic resistance of Extended-Spectrum Beta-Lactamases produci...Premier Publishers
Background: Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae are common in hospitals. This study aims to describe the antibiotic resistance of these bacteria and their associated demographic and clinical factors. Methods: It was a prospective study of 73 isolates of Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae for a period of six months from July to December 2019 in the laboratory of Befelatanana. Results: This study showed 73 (6.3%) isolates of Extended-Spectrum Beta-Lactamases- producing Enterobacteriaceae, represented by 25 (34.2%) isolates of Klebsiella spp, 24 (32.9%) isolates of Escherichia coli, 22 (30.1%) isolates of Enterobacter spp and 2 (2.7%) isolates of Proteus spp. The antibiotic resistance of these bacteria varied from 0% to 100% for all of the antibiotics tested. Resistance to aminoglycosides ranged from 0% (amikacin) to 69.9% (gentamycin). Resistance to quinolones ranged from 43.8% (levofloxacin) to 76.7% (nalidixic acid). Similarly, 60 (82.2%) isolates were resistant to cotrimoxazole and 25 (34.2%) isolates to chloramphenicol. Patients under 20 years (57.1%) (p=0.03), men (52.2%)(p=0.11; NS), patients with respiratory samples (83.3%)(p=0.004), with pus (61.9%)(p=0.02) and hospitalized in surgery and intensive care units (68.4%)(p=0.0009) were the most affected by these enterobacteria. Conclusion: Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae are responsible for severe infections and the majorities are multi-resistant bacteria.
Keywords: Beta-lactamase, Enterobacteriaceae, antibiotic resistance, amikacin, imipenem.
In vitro assessment of antibacterial activity of Salicornia herbacea L. seed ...Innspub Net
In this study, the antibacterial activities of Salicornia herbacea L. seed extract against two gram-negative and two gram- positive bacteria were evaluated with the agar disc diffusion and MIC methods. Result showed that inhibition zones of 9.5±0.01, 6.2±0.00, 4±0.00 and 3.5±0.10 mm for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli, respectively. Among four bacteria the maximum and minimum inhibition seed ethanolic extract were related to S. aureus with inhibition zones of 9.5mm and MIC 189.5 mg/ml and E. coli with inhibition zones of 3.5 mm and MIC 420 mg/ml, respectively. The antimicrobial activity of ethanol seed extract of S. herbacea is the result of phenolic compounds, fatty acids, osmotic compound (betaine)
or synergic and additive effect of several compounds present in it. Our results suggest the possibility of using S. herbacea seed, which possesses strong antibacterial activity, in the treatment of diseases caused by the microorganisms tested. Get the full articles at: http://www.innspub.net/volume-4-number-6-march-2014/
Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimu...ijtsrd
In this study, clinical isolate of Staphylococcus aureus was subjected to susceptibility tests against commercially available antibiotics and Ocimum gratissimum scent leaf leaf extracts. disc diffusion nmethod was adopted for the antibiotics test while agar well diffusion technique was employed for the antimicrobial screening of the leaf extracts. The result shows that the organism was sensitive to Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing 40 of the antibiotics while showing outright resistance to Ampiclox, Amoxicillin and Erythromycin 30 and intermediate results for the rest. Also, the cold water extract of O. gratissimum did not show any activity against the bacterium. However, both hot water extract and ethanol extract of the plant had varying degree of activity against the organism with ethanol extract recording a zone of inhibition of 25.33 mm compared with 32.00 mm recorded for Gentamycin. Furthermore, the minimum inhibitory concentration recorded ofr the extract against the organism were 10 mg ml and 2.5 mg ml respectively for hot water and ethanol extracts. These results indicate that ethanol extract of O. gratissimum leaf is very effective against Staphylococcus aureus and could be used as potential source of natural product for the treatment of infections caused by the organism especially the antibiotic resistant strains. Komolafe T. O. | Ogunyankin O. G "Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46333.pdf Paper URL : https://www.ijtsrd.com/other-scientific-research-area/other/46333/antibiogram-of-staphylococcus-aureus-and-its-sensitivity-to-ocimum-gratissimum-extract/komolafe-t-o
Protein was extracted from muscles of Channa striatus and attempts were
made to evaluate in vitro antibacterial activity against clinical bacterial isolates. The
higher concentration of protein (100μg/ml) extracts exhibited a pronounced activity
against Pseudomonas aeruginosa (21 mm), Proteus vulgaris (19 mm), Citrobacter sp
(19 mm), Klebsiella pneumoniae (18 mm), Micrococcus sp (17 mm), Bacillus subtilis (16
mm), Staphylococcus aureus (15 mm), E. coli (14 mm) and Serratia marcescens (5
mm). The minimum inhibitory concentration and minimum bactericidal concentration
were found to be 20-40 μg/ml and 80-100 μg/ml respectively for the extracts of
Channa striatus protein against test organisms. This study confirms that C. striatus fish
protein extracts possess antibacterial activity against a wide range of microbes and
justified that it could be used in the traditional medicine as a remedy for the
treatment of bacterial diseases.
A study of antibiotic resistance of Extended-Spectrum Beta-Lactamases produci...Premier Publishers
Background: Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae are common in hospitals. This study aims to describe the antibiotic resistance of these bacteria and their associated demographic and clinical factors. Methods: It was a prospective study of 73 isolates of Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae for a period of six months from July to December 2019 in the laboratory of Befelatanana. Results: This study showed 73 (6.3%) isolates of Extended-Spectrum Beta-Lactamases- producing Enterobacteriaceae, represented by 25 (34.2%) isolates of Klebsiella spp, 24 (32.9%) isolates of Escherichia coli, 22 (30.1%) isolates of Enterobacter spp and 2 (2.7%) isolates of Proteus spp. The antibiotic resistance of these bacteria varied from 0% to 100% for all of the antibiotics tested. Resistance to aminoglycosides ranged from 0% (amikacin) to 69.9% (gentamycin). Resistance to quinolones ranged from 43.8% (levofloxacin) to 76.7% (nalidixic acid). Similarly, 60 (82.2%) isolates were resistant to cotrimoxazole and 25 (34.2%) isolates to chloramphenicol. Patients under 20 years (57.1%) (p=0.03), men (52.2%)(p=0.11; NS), patients with respiratory samples (83.3%)(p=0.004), with pus (61.9%)(p=0.02) and hospitalized in surgery and intensive care units (68.4%)(p=0.0009) were the most affected by these enterobacteria. Conclusion: Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae are responsible for severe infections and the majorities are multi-resistant bacteria.
Keywords: Beta-lactamase, Enterobacteriaceae, antibiotic resistance, amikacin, imipenem.
In vitro assessment of antibacterial activity of Salicornia herbacea L. seed ...Innspub Net
In this study, the antibacterial activities of Salicornia herbacea L. seed extract against two gram-negative and two gram- positive bacteria were evaluated with the agar disc diffusion and MIC methods. Result showed that inhibition zones of 9.5±0.01, 6.2±0.00, 4±0.00 and 3.5±0.10 mm for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli, respectively. Among four bacteria the maximum and minimum inhibition seed ethanolic extract were related to S. aureus with inhibition zones of 9.5mm and MIC 189.5 mg/ml and E. coli with inhibition zones of 3.5 mm and MIC 420 mg/ml, respectively. The antimicrobial activity of ethanol seed extract of S. herbacea is the result of phenolic compounds, fatty acids, osmotic compound (betaine)
or synergic and additive effect of several compounds present in it. Our results suggest the possibility of using S. herbacea seed, which possesses strong antibacterial activity, in the treatment of diseases caused by the microorganisms tested. Get the full articles at: http://www.innspub.net/volume-4-number-6-march-2014/
Multidrug Resistance Pattern of Staphylococcus Aureus Isolates in Maiduguri M...Scientific Review
Multi drug-resistant (MDR) isolates of Staphylococcus aureus are on rise and are becoming a challenge for timely and appropriate treatment. The present study was carried out with an objective to isolate Staphylococcus aureus from clinical samples and determine their sensitivity. Out of 110 samples collected, 44 were shown to contained S. aureus. The isolates were subjected to antibiotic sensitivity tests using 10 different and commonly used antibiotics by modified Kirby- Bauer disc diffusion technique. Out of the total isolates (42) tested, only 7.1% were susceptible to all the antibiotics. Multiple resistance was eminent in over 92% with highest occurrence in 4.8% where the entire antibiotics were resisted. Multiple antibiotic resistance indixes (MAR index) indicated that 0.6 index occurred most (23.8%) followed by 0.5 (19.0%). On the other hand, 0.1 and 0.8 indexes were the lowest with 0.0% and 1.0% occurrence respectively. Ciprofloxacin was resisted by most of the organisms (64.3%) while amoxicillin (64.3%) and streptomycin (61.9%) were most efficacious. With over 90% isolate having MAR index ≥ 0.2, the multiple drug resistance by the S. aureus is quite alarming and might suggest inappropriate antibiotic usage by the sampled population. Therefore, the need to strategize the nature of antibiotic treatment against S. aureus and massive campaign on indiscriminate antibiotic use is urgent.
Effects of combination of magnesium and zinc oxide nanoparticles and heat on ...Nanomedicine Journal (NMJ)
Objective: The objective of this study was to investigate the antibacterial activities of combination of MgO and ZnO nanoparticles in the presence of heat against Escherichia coli and Staphylococcus aureus.
Materials and Methods:Bacteria were grown on either agar or broth media followed by the addition of ZnO and MgO nanoparticles. Then the combined effect of ZnO and MgO nanoparticles was investigated. Furthermore, the media containing nanoparticles were treated with mild heat and their synergistic antibacterial activity was investigated against E. coli and S. aureus in milk.
Results: The data showed that the nanoparticles used in this study had no effect on the bacteria in the agar medium. However, the results showed that ZnO and MgO nanoparticles resulted in a significant decrease in the number of E. coli (P<0.000) and S. aureus (Pd”0.05) in the broth medium. The combination of nanoparticles and mild heat exhibited a significant decrease in the number of E. coli and S. aureus indicating the synergistic effects of nanoparticles and heat.
Conclusion: Using a combination of mild heat, ZnO and MgO nanoparticles, E. coli and S. aureus can be controlled successfully in the milk. Mild heating plus ZnO and MgO nanoparticles has a synergistic effect which would reduce the need for high temperature and also the concentrations of ZnO and MgO nanoparticles required for pathogen control in minimally processed milk during maintaining.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Biological Efficacy of Quassia Indica (Geratn) Nooteb and Centella Asiatica (...IOSRJPBS
Antimicrobial activity of different extracts of two selected plants, Quassia indica (Geartn) Nooteb and Centella asiatica (L.) Urban were tested against selected strains of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas sps, Bacillus thuringiensis, Klebsiella sps and Serratia sps using agar disc diffusion technique. Petroleum ether, acetone, methanol and water extracts of seed and leaf of Q. indica and leaf of C. asiatica showed moderate to significant antimicrobial activity. Of the four extracts tested, petroleum ether and water fractions showed more activity against all the bacterial strains. The zone of inhibition of the active fractions for the petroleum ether ranged from 8.57 to 12.63 mm and 8.53 to 14.5mm for the water fraction of Q. indica seed and leaf and C. asiatica leaf; and exhibited comparable results with widely used commercial antimicrobial agents (16 to19 mm for Kanamycin and 14 to 17mm for Cefotaxime). The results prove the efficacy of the most active fractions of the selected plant extracts to be used for developing potent antimicrobial formulations.
This poster was presented at the 2015 Georgia Bio Conference in Atlanta, GA.
Abstract:
Alarming trends in the spread of antibiotic resistance among top pathogens, including Staphylococcus aureus, have pushed mankind toward what has been coined as the “post-antibiotic era.” Therefore, an indirect attack on bacteria through interfering with their means of communication, quorum sensing, is proposed. An underappreciated source for modern anti-infectives is natural products from terrestrial plants. A rich history of medical traditions developed under the influence of diverse cultures in the Mediterranean and many of these are still practiced by local people. Investigation of botanical folk medicines used in the treatment of skin and soft tissue infections identified Castanea sativa (European Chestnut) for its potential antibacterial activity.
This work demonstrates the quorum sensing inhibitory activity of oleanene and ursene derivatives from a C. sativa leaf extract against all S. aureus accessory gene regulator (agr) alleles. Multiple layers of evidence for agr blocking activity (IC50 1.56-25 µg mL-1) are reported: toxin outputs, reporter assays, hemolytic activity, cytotoxicity studies, and an in vivo abscess model. The C. sativa extract is neither cytotoxic to human keratinocytes, nor murine skin; it neither inhibits S. aureus growth, nor skin commensal growth. Serial passaging experiments with the extract did not result in the development of resistance. In conclusion, the disruption of quorum sensing in the absence of growth inhibition demonstrated by this natural product derived non-biocidal inhibitor of virulence shows potential for future antibiotic therapies.
Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The agar diffusion assay of these flavonoids with different anti-fungal agents was tested. The Minimum Inhibitory Concentration (MIC) values of each of the flavonoids with different anti-fungal agents were determined by using checkerboard broth micro dilution assay. Flavones diglycoside (3, 5-dihydroxy flavones 7-O-b-D-glucuronide-4-O-b-D-glucopyranside) potentiated the in vitro and in vivo activity against fungal strains. The flavones diglycoside reduced MIC of amphotericin-B to one half against different fungal strains, Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus neoformans 1202. Although moderate change between in vitro and in vivo studies have been found, the elucidation of the mechanisms involved in flavonoid action will have many health benefits to man. In conclusion, these findings suggested that flavonoid combination regimens may be considered as an useful candidate for the treatment of fungal infection.
Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concent...IOSRJPBS
MIC methods are widely used in the comparative testing of new agents, or when a more accurate result is required for clinical management. As there are no CLSI (formerly NCCLS) recommendations for the determination of MICs of varies bio-compounds against gram positive and gram negative organisms. The minimum inhibitory concentration is the lowest concentration (in μg/ml) of an antibiotic that inhibits the growth of a given strain of bacteria. In Diagnostic laboratories these MIC’S are used to confirm resistance and also most often used to determine antimicrobial invitro activity. In the present MIC method, one can get the information about Antibiotic agent, preparation of stock solutions, media and inoculation, conditions of incubation, ultimately reading and interpretation of results. The present study is focused to evaluate antibacterial activities of ethanolic extract and purified fractions of Bauhinia purpurea using a gram-positive and a gram-negative organisms Staphylococcus aureus , Klebsiella, E.coli and enterococcus
Synergistic antibacterial effects of three edible plants extract against anti...Open Access Research Paper
In vitro synergistic antibacterial effects among Alocasia macrorrhizos rhizome, Amorphophallus paeoniifolius corm and Colocasia esculenta corm extracts were tested against six resistant bacteria viz., Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile and Staphylococcus aureus. The inhibition zone was compared with the commercially available antibiotic (tetracycline). High inhibitory activity was observed against E. coli (12.67±0.33 mm) and S. aureus (12.50±0.29 mm) for methanol extract at 800 mgml-1 of concentration. MIC and MBC of the extracts ranged from 200-580 mgml-1 and 250-650 mgml-1 respectively. The lowest MIC and MBC of the extracts were measured against E. coli.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
In vitro controlling of selected human diarrhea causing bacteria by clove ext...Open Access Research Paper
Antibacterial activity of clove extracts (Syzygium aromaticum L.) was proven against five diarrhea causing bacteria. This was further confirmed when compared with commonly used three commercial antibiotics (ciprofloxacin, tetracycline and erythromycin) as a positive control. Significant differences (P<0.0001) were observed in the effect of the antimicrobial agents (clove extracts and antibiotics), and in the sensitivities of the bacterial species (P<0.0001) to the antimicrobial agents. Clove extracts had significant (P<0.001) activity with the acetone extract demonstrating highest activity followed by antibiotics and other extracts against tested bacteria. The zone of inhibition of clove extracts was ranged from 7.33 to 12.00 mm whereas in antibiotics, it was 0.00 to 11.67 mm. Of all the bacteria, Salmonella typhimurium was the most susceptible against all of the extracts as well as concentrations of clove, while low MIC (180 mgml-1) and MBC (680 mgml-1) of the extracts were observed against Shigella dysenteriae. Consequently, clove has a significant antidiarrheal activity and it could be used as an effective antibacterial agent, alternative to the use of antibiotics.
The aim of the study was to evaluate the antibacterial evaluation of root extracts of Juglans regia against Extended Spectrum Beta Lactamase (ESBL) producing E. coli and Klebsiella pneumonia in Bombay Hospital and Research Centre Jabalpur. The antibacterial activity of, ethyl acetate and methanol root extracts of Juglans regia was determined by disk diffusion method. The antibacterial activity was calculated based on the minimum inhibitory concentration using Mueller–Hinton broth in a tube-dilution method. The best antibacterial activity, calculated as minimum inhibitory concentration values, against ESBL was shown by the methanol root extract Juglans regia (25 mg/mL) for both isolated organisms and ethyl acetate (25mg/mL) against E. coli. The methanol extract showed zone of inhibition in the range of 17-26mm as compared to ethyl acetate extract which showed zone of inhibition in the range of 11-16mm against the uropathogens. The zone of inhibition ranged from 17 mm to 26 mm and MIC was 25mg/ml. This effect is comparable to antibiotics. The results obtained in the present study suggest that Juglans regia have the potential to be developed as antibacterial agents against ESBL producing UTI bacteria strain. Further investigations are needed to identify the active compounds and their mechanism of action
Multidrug Resistance Pattern of Staphylococcus Aureus Isolates in Maiduguri M...Scientific Review
Multi drug-resistant (MDR) isolates of Staphylococcus aureus are on rise and are becoming a challenge for timely and appropriate treatment. The present study was carried out with an objective to isolate Staphylococcus aureus from clinical samples and determine their sensitivity. Out of 110 samples collected, 44 were shown to contained S. aureus. The isolates were subjected to antibiotic sensitivity tests using 10 different and commonly used antibiotics by modified Kirby- Bauer disc diffusion technique. Out of the total isolates (42) tested, only 7.1% were susceptible to all the antibiotics. Multiple resistance was eminent in over 92% with highest occurrence in 4.8% where the entire antibiotics were resisted. Multiple antibiotic resistance indixes (MAR index) indicated that 0.6 index occurred most (23.8%) followed by 0.5 (19.0%). On the other hand, 0.1 and 0.8 indexes were the lowest with 0.0% and 1.0% occurrence respectively. Ciprofloxacin was resisted by most of the organisms (64.3%) while amoxicillin (64.3%) and streptomycin (61.9%) were most efficacious. With over 90% isolate having MAR index ≥ 0.2, the multiple drug resistance by the S. aureus is quite alarming and might suggest inappropriate antibiotic usage by the sampled population. Therefore, the need to strategize the nature of antibiotic treatment against S. aureus and massive campaign on indiscriminate antibiotic use is urgent.
Effects of combination of magnesium and zinc oxide nanoparticles and heat on ...Nanomedicine Journal (NMJ)
Objective: The objective of this study was to investigate the antibacterial activities of combination of MgO and ZnO nanoparticles in the presence of heat against Escherichia coli and Staphylococcus aureus.
Materials and Methods:Bacteria were grown on either agar or broth media followed by the addition of ZnO and MgO nanoparticles. Then the combined effect of ZnO and MgO nanoparticles was investigated. Furthermore, the media containing nanoparticles were treated with mild heat and their synergistic antibacterial activity was investigated against E. coli and S. aureus in milk.
Results: The data showed that the nanoparticles used in this study had no effect on the bacteria in the agar medium. However, the results showed that ZnO and MgO nanoparticles resulted in a significant decrease in the number of E. coli (P<0.000) and S. aureus (Pd”0.05) in the broth medium. The combination of nanoparticles and mild heat exhibited a significant decrease in the number of E. coli and S. aureus indicating the synergistic effects of nanoparticles and heat.
Conclusion: Using a combination of mild heat, ZnO and MgO nanoparticles, E. coli and S. aureus can be controlled successfully in the milk. Mild heating plus ZnO and MgO nanoparticles has a synergistic effect which would reduce the need for high temperature and also the concentrations of ZnO and MgO nanoparticles required for pathogen control in minimally processed milk during maintaining.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Biological Efficacy of Quassia Indica (Geratn) Nooteb and Centella Asiatica (...IOSRJPBS
Antimicrobial activity of different extracts of two selected plants, Quassia indica (Geartn) Nooteb and Centella asiatica (L.) Urban were tested against selected strains of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas sps, Bacillus thuringiensis, Klebsiella sps and Serratia sps using agar disc diffusion technique. Petroleum ether, acetone, methanol and water extracts of seed and leaf of Q. indica and leaf of C. asiatica showed moderate to significant antimicrobial activity. Of the four extracts tested, petroleum ether and water fractions showed more activity against all the bacterial strains. The zone of inhibition of the active fractions for the petroleum ether ranged from 8.57 to 12.63 mm and 8.53 to 14.5mm for the water fraction of Q. indica seed and leaf and C. asiatica leaf; and exhibited comparable results with widely used commercial antimicrobial agents (16 to19 mm for Kanamycin and 14 to 17mm for Cefotaxime). The results prove the efficacy of the most active fractions of the selected plant extracts to be used for developing potent antimicrobial formulations.
This poster was presented at the 2015 Georgia Bio Conference in Atlanta, GA.
Abstract:
Alarming trends in the spread of antibiotic resistance among top pathogens, including Staphylococcus aureus, have pushed mankind toward what has been coined as the “post-antibiotic era.” Therefore, an indirect attack on bacteria through interfering with their means of communication, quorum sensing, is proposed. An underappreciated source for modern anti-infectives is natural products from terrestrial plants. A rich history of medical traditions developed under the influence of diverse cultures in the Mediterranean and many of these are still practiced by local people. Investigation of botanical folk medicines used in the treatment of skin and soft tissue infections identified Castanea sativa (European Chestnut) for its potential antibacterial activity.
This work demonstrates the quorum sensing inhibitory activity of oleanene and ursene derivatives from a C. sativa leaf extract against all S. aureus accessory gene regulator (agr) alleles. Multiple layers of evidence for agr blocking activity (IC50 1.56-25 µg mL-1) are reported: toxin outputs, reporter assays, hemolytic activity, cytotoxicity studies, and an in vivo abscess model. The C. sativa extract is neither cytotoxic to human keratinocytes, nor murine skin; it neither inhibits S. aureus growth, nor skin commensal growth. Serial passaging experiments with the extract did not result in the development of resistance. In conclusion, the disruption of quorum sensing in the absence of growth inhibition demonstrated by this natural product derived non-biocidal inhibitor of virulence shows potential for future antibiotic therapies.
Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The agar diffusion assay of these flavonoids with different anti-fungal agents was tested. The Minimum Inhibitory Concentration (MIC) values of each of the flavonoids with different anti-fungal agents were determined by using checkerboard broth micro dilution assay. Flavones diglycoside (3, 5-dihydroxy flavones 7-O-b-D-glucuronide-4-O-b-D-glucopyranside) potentiated the in vitro and in vivo activity against fungal strains. The flavones diglycoside reduced MIC of amphotericin-B to one half against different fungal strains, Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus neoformans 1202. Although moderate change between in vitro and in vivo studies have been found, the elucidation of the mechanisms involved in flavonoid action will have many health benefits to man. In conclusion, these findings suggested that flavonoid combination regimens may be considered as an useful candidate for the treatment of fungal infection.
Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concent...IOSRJPBS
MIC methods are widely used in the comparative testing of new agents, or when a more accurate result is required for clinical management. As there are no CLSI (formerly NCCLS) recommendations for the determination of MICs of varies bio-compounds against gram positive and gram negative organisms. The minimum inhibitory concentration is the lowest concentration (in μg/ml) of an antibiotic that inhibits the growth of a given strain of bacteria. In Diagnostic laboratories these MIC’S are used to confirm resistance and also most often used to determine antimicrobial invitro activity. In the present MIC method, one can get the information about Antibiotic agent, preparation of stock solutions, media and inoculation, conditions of incubation, ultimately reading and interpretation of results. The present study is focused to evaluate antibacterial activities of ethanolic extract and purified fractions of Bauhinia purpurea using a gram-positive and a gram-negative organisms Staphylococcus aureus , Klebsiella, E.coli and enterococcus
Synergistic antibacterial effects of three edible plants extract against anti...Open Access Research Paper
In vitro synergistic antibacterial effects among Alocasia macrorrhizos rhizome, Amorphophallus paeoniifolius corm and Colocasia esculenta corm extracts were tested against six resistant bacteria viz., Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile and Staphylococcus aureus. The inhibition zone was compared with the commercially available antibiotic (tetracycline). High inhibitory activity was observed against E. coli (12.67±0.33 mm) and S. aureus (12.50±0.29 mm) for methanol extract at 800 mgml-1 of concentration. MIC and MBC of the extracts ranged from 200-580 mgml-1 and 250-650 mgml-1 respectively. The lowest MIC and MBC of the extracts were measured against E. coli.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
In vitro controlling of selected human diarrhea causing bacteria by clove ext...Open Access Research Paper
Antibacterial activity of clove extracts (Syzygium aromaticum L.) was proven against five diarrhea causing bacteria. This was further confirmed when compared with commonly used three commercial antibiotics (ciprofloxacin, tetracycline and erythromycin) as a positive control. Significant differences (P<0.0001) were observed in the effect of the antimicrobial agents (clove extracts and antibiotics), and in the sensitivities of the bacterial species (P<0.0001) to the antimicrobial agents. Clove extracts had significant (P<0.001) activity with the acetone extract demonstrating highest activity followed by antibiotics and other extracts against tested bacteria. The zone of inhibition of clove extracts was ranged from 7.33 to 12.00 mm whereas in antibiotics, it was 0.00 to 11.67 mm. Of all the bacteria, Salmonella typhimurium was the most susceptible against all of the extracts as well as concentrations of clove, while low MIC (180 mgml-1) and MBC (680 mgml-1) of the extracts were observed against Shigella dysenteriae. Consequently, clove has a significant antidiarrheal activity and it could be used as an effective antibacterial agent, alternative to the use of antibiotics.
The aim of the study was to evaluate the antibacterial evaluation of root extracts of Juglans regia against Extended Spectrum Beta Lactamase (ESBL) producing E. coli and Klebsiella pneumonia in Bombay Hospital and Research Centre Jabalpur. The antibacterial activity of, ethyl acetate and methanol root extracts of Juglans regia was determined by disk diffusion method. The antibacterial activity was calculated based on the minimum inhibitory concentration using Mueller–Hinton broth in a tube-dilution method. The best antibacterial activity, calculated as minimum inhibitory concentration values, against ESBL was shown by the methanol root extract Juglans regia (25 mg/mL) for both isolated organisms and ethyl acetate (25mg/mL) against E. coli. The methanol extract showed zone of inhibition in the range of 17-26mm as compared to ethyl acetate extract which showed zone of inhibition in the range of 11-16mm against the uropathogens. The zone of inhibition ranged from 17 mm to 26 mm and MIC was 25mg/ml. This effect is comparable to antibiotics. The results obtained in the present study suggest that Juglans regia have the potential to be developed as antibacterial agents against ESBL producing UTI bacteria strain. Further investigations are needed to identify the active compounds and their mechanism of action
Antibacterial Activity of Stem Bark Extracts of Oroxylum indicum an Endangere...IOSR Journals
The present work has been under taken to study the antibacterial activity of stem bark extracts of O.
indicum against disease causing gram negative and gram positive bacteria. Antimicrobial activity of solvent
extracts of stem bark of Oroxylum indicum has been studied to find out its activity against four important
bacterial strains Bacillus subtilis, B. cereus, Staphylococcus albus and S. aureus . The antimicrobial activity of
the stem bark extracts was done through well diffusion method and by measuring the inhibition zone around the
disc. The results revealed that the aqueous extracts of O. indicum exhibited antimicrobial activity against all the
microbes under study. The results provided evidence that the species O. indicum can be used as a potential
source of antimicrobial agent.
Multidrug Resistance Pattern of Staphylococcus Aureus Isolates in Maiduguri ...Scientific Review SR
Multi drug-resistant (MDR) isolates of Staphylococcus aureus are on rise and are becoming a
challenge for timely and appropriate treatment. The present study was carried out with an objective to isolate
Staphylococcus aureus from clinical samples and determine their sensitivity. Out of 110 samples collected, 44
were shown to contained S. aureus. The isolates were subjected to antibiotic sensitivity tests using 10 different
and commonly used antibiotics by modified Kirby- Bauer disc diffusion technique. Out of the total isolates (42)
tested, only 7.1% were susceptible to all the antibiotics. Multiple resistance was eminent in over 92% with
highest occurrence in 4.8% where the entire antibiotics were resisted. Multiple antibiotic resistance indixes
(MAR index) indicated that 0.6 index occurred most (23.8%) followed by 0.5 (19.0%). On the other hand, 0.1
and 0.8 indexes were the lowest with 0.0% and 1.0% occurrence respectively. Ciprofloxacin was resisted by
most of the organisms (64.3%) while amoxicillin (64.3%) and streptomycin (61.9%) were most efficacious. With
over 90% isolate having MAR index ≥ 0.2, the multiple drug resistance by the S. aureus is quite alarming and
might suggest inappropriate antibiotic usage by the sampled population. Therefore, the need to strategize the
nature of antibiotic treatment against S. aureus and massive campaign on indiscriminate antibiotic use is urgent.
Antipyretic, Antimicrobial and Anti-inflammatory activity of Kabasura kudinee...GOPALASATHEESKUMAR K
This study is designed to evaluate the anti-inflammaatory, antipyretic aqueous extract of Kabasurakudineerchoornam (AEKKC). The estimated by Folin-Ciocalteu and Aluminium chloride colorimetric potential was determined by DPPH and ABTS radical scavenging any signs of toxicity upto 2000 mg/kg in rats. The anti-inflammatory evaluated by carrageenan-induced paw edema and histamine induced of AEKKC at 200 and 400 mg/kg showed significant reduction in both carrageenan and histamine induced inflammation. The and 400 mg/kg) was studied by brewer’s yeast induced pyrexia mg/kg) was used as standard. The extract showed significant evoked elevated body temperature. AEKKC also exhibited antibacterial inhibition via disc diffusion method. The results of the study prove inflammatory, antipyretic and antimicrobial activity.
Invitro Evaluation of Antimicrobial Activity of the plant extracts of Elytrar...IJERA Editor
Antimicrobial activity of the aerial parts of the Elytraria acaulis a stem less perennial herb of Acantheceae family has been carried out in the present study. Extracts of the aerial parts of the plant (Stem & Leaves) were prepared in different organic solvents such as n-Hexane, Ethanol, Methanol and Ethyl acetate. All the extracts were analyzed for antimicrobial properties against various pathogenic bacterial infections (Escherichia coli, Klebsiella Species, and Salmonella Typhi). Amongst all the extracts, methanolic extract exhibited significant antimicrobial activity. The crude methanol extracts of leaves showed good inhibitory effects against pathogens. Hence further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications.
Isolation and Screening of Soil Actinomycetes for Antimicrobial Activity OlakunleAjibola
Antimicrobial resistance in pathogens has greatly increased of late and now pose a serious public health problem globally. New antimicrobials are continuously needed to inhibit the growth of these resistant strains.
The aim of this study was to isolate and screen soil actinomycetes and evaluate their secondary metabolites for antimicrobial activities against selected pathogenic bacteria and fungi.
Antibiotic Susceptibility Pattern of Pyogenic Bacterial Isolates in Sputum.IOSR Journals
Drugs Have Been Used For The Treatment Of Infectious Diseases Since 17th Century , However
Chemotherapy As A Science Has Began With Paul Ehrlich In The First Decade Of 20th Century . Paul Ehrlich
(1854-1915) Was One Of The Earliest Pioneers In The Field Of Antimicrobial Chemotherapy .1Ehrlich
Formulated The Principles Of “Selective Toxicity” ,I.E; Selective Inhibition Of The Growth Of Microorganisms
Without Damage To The Host.2 Resistance Has Been Documented Not Only Against Antibiotics Of
Natural And Semi- Synthetic Origin , But Also Against Purely Synthetic Compounds (Flouroquinolone) Or
Those Which Do Not Even Enter The Cells (Vancomycin) .3 However , The Euphoria Over The Potential
Conquest Of Infectious Diseases Was Short-Lived .Almost As Soon As Antibacterial Drugs Were Deployed ,
Bacteria Responded By Manifesting Various Forms Of Resistance.4 Considered As “Wonder Drugs”
Antibiotics Are Often Prescribed Inappropriately And Inadequately And Have Thus Became One Of The
Highly Abused Agents.5
Evaluation of Agro-morphological Performances of Hybrid Varieties of Chili Pe...Premier Publishers
In Benin, chilli pepper is a widely consumed as vegetable whose production requires the use of performant varieties. This work assessed, at Parakou and Malanville, the performance of six F1 hybrids of chilli including five imported (Laali, Laser, Nandi, Kranti, Nandita) and one local (De cayenne), in completely randomized block design at four replications and 15 plants per elementary plot. Agro-morphological data were collected and submitted to analysis of variance and factor analysis of mixed data. The results showed the effects of variety, location and their interactions were highly significant for most of the growth, earliness and yield traits. Imported hybrid varieties showed the best performances compared to the local one. Multivariate analysis revealed that 'De cayenne' was earlier, short in size, thin-stemmed, red fruits and less yielding (≈ 1 t.ha-1). The imported hybrids LaaliF1 and KrantiF1 were of strong vegetative vigor, more yielding (> 6 t.ha-1) by developing larger, long and hard fruits. Other hybrids showed intermediate performances. This study highlighted the importance of imported hybrids in improving yield and preservation of chili fruits. However, stability and adaptation analyses to local conditions are necessary for their adoption.
An Empirical Approach for the Variation in Capital Market Price Changes Premier Publishers
The chances of an investor in the stock market depends mainly on some certain decisions in respect to equilibrium prices, which is the condition of a system competing favorably and effectively. This paper considered a stochastic model which was latter transformed to non-linear ordinary differential equation where stock volatility was used as a key parameter. The analytical solution was obtained which determined the equilibrium prices. A theorem was developed and proved to show that the proposed mathematical model follows a normal distribution since it has a symmetric property. Finally, graphical results were presented and the effects of the relevant parameters were discussed.
Influence of Nitrogen and Spacing on Growth and Yield of Chia (Salvia hispani...Premier Publishers
Chia is an emerging cash crop in Kenya and its production is inhibited by lack of agronomic management information. A field experiment was conducted in February-June and May-August 2021, to determine the influence of nitrogen and spacing on growth and yield of Chia. A randomized complete block design with a split plot arrangement was used with four nitrogen rates as the main plots (0, 40, 80, 120 kg N ha-1) and three spacing (30 cm x 15 cm (s1), 30 cm x 30 cm (s2), 50 cm x 50 cm (s3)). Application of 120 kg N ha-1 significantly increased (p≤0.05) vegetative growth and seed yield of Chia. Stem height, branches, stem diameter and leaves increased by 23-28%, 11-13%, 43-55% and 59-88% respectively. Spacing s3 significantly increased (p≤0.05) vegetative growth. An increase of 27-74%, 36-45% and 73-107% was recorded in number of leaves, stem diameter and dry weight, respectively. Chia yield per plant was significantly higher (p≤0.05) in s3. However, when expressed per unit area, s1 significantly produced higher yields. The study recommends 120 kg N ha-1 or higher nitrogen rates and a closer spacing of 15 cm x 30 cm as the best option for Chia production in Kenya.
Enhancing Social Capital During the Pandemic: A Case of the Rural Women in Bu...Premier Publishers
Social capital plays an essential role in empowering people for social and economic change even during the pandemic. A livelihood project of the government was implemented among the members of a women’s association of a disadvantaged upland community in Bukidnon province, Southern Philippines for inclusive development. This study was conducted to determine the influence of some socio-economic attributes and the change in the knowledge level on the social capital of the rural women amidst the pandemic. The activities of the project were implemented considering the health protocols imposed by the government during the health crisis. The findings revealed that the trainings conducted resulted to a positive change in the knowledge level among the rural women. This facilitated the production of vegetables for their households and generated additional income very necessary during the pandemic especially that other economic activities were hindered. Similarly, there was a significant increase in the social capital of the rural women during the last two years. The main occupation, sources of income and their ethnicity significantly influenced the social capital of the rural women. The rural development workers and policymakers must consider the social capital of the group in the implementation of poverty alleviation programs.
Impact of Provision of Litigation Supports through Forensic Investigations on...Premier Publishers
This paper presents an argument through the fraud triangle theory that the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases is adequate for effective prosecution of perpetrators as well as corporate fraud prevention. To support this argument, this study operationalized provision of litigation supports through forensic audit and investigations, data mining for trends and patterns, and fraud data collection and preparation. A sample of 500 respondents was drawn from the population of professional accountants and legal practitioners in Nigeria. Questionnaire was used as the instrument for data collection and this was mailed to the respective respondents. Resulting responses were analyzed using the OLS multiple regression techniques via the SPSS statistical software. The results reveal that the provision of litigation supports through forensic audits and investigations, fraud data mining for trends and patterns and fraud data collection and preparation for court proceedings have a positive and significant impact on corporate fraud prevention in Nigeria. This study therefore recommends that regulators should promote the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases in publicly listed firms in Nigeria, as this will help provide reports that are acceptable in court proceedings.
Improving the Efficiency of Ratio Estimators by Calibration WeightingsPremier Publishers
It is observed that the performances of most improved ratio estimators depend on some optimality conditions that need to be satisfied to guarantee better estimator. This paper develops a new approach to ratio estimation that produces a more efficient class of ratio estimators that do not depend on any optimality conditions for optimum performance using calibration weightings. The relative performances of the proposed calibration ratio estimators are compared with a corresponding global [Generalized Regression (GREG)] estimator. Results of analysis showed that the proposed calibration ratio estimators are substantially superior to the traditional GREG-estimator with relatively small bias, mean square error, average length of confidence interval and coverage probability. In general, the proposed calibration ratio estimators are more efficient than all existing estimators considered in the study.
Urban Liveability in the Context of Sustainable Development: A Perspective fr...Premier Publishers
Urbanization and quality of urban life are mutually related and however it varies geographically and regionally. With unprecedented growth of urban centres, challenge against urban development is more in terms of how to enhance quality of urban life and liveability. Making sense of and measuring urban liveability of urban places has become a crucial step in the context of sustainable development paradigm. Geographical regions depict variations in nature of urban development and consequently level of urban liveability. The coastal regain of West Bengal faces unusual challenges caused by increasing urbanization, uncontrolled growth, and expansion of economic activities like tourism and changing environmental quality. The present study offers a perspective on urban liveability of urban places located in coastal region comprising of Purba Medinipur and South 24 Parganas districts. The study uses the liveability standards covering four major pillars- institutional, social, economic and physical and their indicators. This leads to develop a City Liveability Index to rank urban places of the region, higher the index values better the urban liveability. The data for the purpose is collected from various secondary sources. Study finds that the eastern coastal region of the country covering state of West Bengal depicts variations in index of liveability determined by physical, economic, social and institutional indicators.
Transcript Level of Genes Involved in “Rebaudioside A” Biosynthesis Pathway u...Premier Publishers
Stevia rebaudiana Bertoni is a plant which has recently been used widely as a sweetener. This medicinal plant has some components such as diterpenoid glycosides called steviol glycosides [SGs]. Rebaudioside A is a diterpenoid steviol glycoside which is 300 times sweeter than table sugar. This study was done to investigate the effect of GA3 (50 mg/L) on the expression of 14 genes involved in Rebaudioside A biosynthesis pathway in Stevia rebaudiana under in vitro conditions. The expression of DXS remarkably decreased by day 3. Also, probably because of the negative feedback of GA3 on MEP-drived isoprenes, GGDS transcript level reached its lowest amount after GA3 treatment. The abundance of DXR, CMS, CMK, MCS, and CDPS transcripts showed a significant increase at various days after this treatment. A significant drop in the expression levels of KS and UGT85C2 is detected during the first day. However, expression changes of HDR and KD were not remarkable. Results revealed that the level of transcript of UGT74G1 and UGT76G1 up regulated significantly 4 and 2 times higher than control, respectively. However, more research needs to shed more light on the mechanism of GA3 on gene expression of MEP pathway.
Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Mor...Premier Publishers
Information on genetic variability for biochemical characters is a prerequisite for improvement of tea quality. Thirteen introduced tea clones characterized with objective; assessing tea clones based on morphological characters at Melko and Gera research stations. The study was conducted during 2017/18 cropping season on experimental plots in RCBD with three replications. Data recorded on morphological traits like days from pruning to harvest, height to first branch, stem diameter, leaf serration density, leaf length, leaf width, leaf size, petiole length, leaf ratio, internode length, shoot length, number of shoot, canopy diameter, hundred shoot weight, fresh leaf yield per tree. Cluster analysis of morphological trait grouped into four clusters indicated, the existence of divergence among the tested clones. The maximum inter-cluster distance was between clusters I and IV (35.27) while the minimum inter cluster distance was observed between clusters I and II (7.8).Principal components analysis showed that the first five principal components with eigenvalues greater than one accounted 86.45% for 15 morphological traits. Generally, the study indicated presence of variability for several morphological traits. However, high morphological variation between clones is not a guarantee for a high genetic variation; therefore, molecular studies need to be considered as complementary to biochemical studies.
Causes, Consequences and Remedies of Juvenile Delinquency in the Context of S...Premier Publishers
This research work was designed to examine nature of juvenile offences committed by juveniles, causes of juvenile delinquency, consequences of juvenile delinquency and remedies for juvenile delinquency in the context of Sub-Saharan Africa with specific reference to Eritrea. Left unchecked, juvenile delinquents on the streets engage in petty theft, take alcohol or drugs, rape women, rob people at night involve themselves in criminal gangs and threaten the public at night. To shed light on the problem of juvenile delinquency in the Sub-Saharan region data was collected through primary and secondary sources. A sample size of 70 juvenile delinquents was selected from among 112 juvenile delinquents in remand at the Asmara Juvenile Rehabilitation Center in the Eritrean capital. The study was carried out through coded self-administered questionnaires administered to a sample of 70 juvenile delinquents. The survey evidence indicates that the majority of the juvenile respondents come either from families constructed by unmarried couples or separated or divorced parents where largely the father is missing in the home or dead. The findings also indicate that children born out of wedlock, families led by single mothers, lack of fatherly role models, poor parental-child relationships and negative peer group influence as dominant causes of juvenile infractions. The implication is that broken and stressed families are highly likely to be the breeding grounds for juvenile delinquency. The survey evidence indicates that stealing, truancy or absenteeism from school, rowdy or unruly behavior at school, free-riding in public transportation, damaging the book of fellow students and beating other young persons are the most common forms of juvenile offenses. It is therefore, recommended that parents and guardians should exercise proper parental supervision and give adequate care to transmit positive societal values to children. In addition, the government, the police, prosecution and courts, non-government organizations, parents, teachers, religious leaders, education administrators and other stakeholders should develop a child justice system that strives to prevent children from entering deeper into the criminal justice process.
The Knowledge of and Attitude to and Beliefs about Causes and Treatments of M...Premier Publishers
Stigma and discrimination associated with mental illness are a common occurrence in the Sub-Saharan region including Eritrea. Numerous studies from Sub-Saharan Africa suggest that stigma and discrimination are major problems in the community, with negative attitudes and behavior towards people with mental illness being widespread. In order to assess the whether such negative attitudes persist in the context of Eritrea this study explored the knowledge and perceptions of 90 Eritrean university students at the College of Business and Economics, the University of Asmara regarding the causes and remedies of mental illness A qualitative method involving coded self-administered questionnaires administered to a sample of 90 university students to collecting data at the end of 2019. The survey evidence points that almost 50% of the respondents had contact with a mentally ill person suggesting that the significant number of the respondents experienced a first-hand encounter and knowledge of mental illness in their family and community. The findings show an overall greater science-based understanding of the causes of mental illness to be followed by recommended psychiatric treatments. The survey evidence indicates that the top three leading causes of mental illness in the context of Eritrea according to the respondents are brain disease (76%), bad events in the life of the mentally ill person (66%) and substance abuse or alcohol taking, smoking, taking drugs like hashish. (54%). The majority of the respondents have a very sympathetic and positive outlook towards mentally ill persons suggesting that mentally illness does not simply affect a chosen individual rather it can happen to anybody regardless of economic class, social status, ethnicity race and religion. Medical interventions cited by the majority of the respondents as being effective treatments for mental illness centered on the idea that hospitals and clinics for treatment and even cures for psychiatric disease. Changing perceptions of mental illnesses in Eritrea that paralleled the very caring and sympathetic attitudes of the sample university students would require raising public awareness regarding mental illness through education, using the mass media to raise public awareness, integrating mental health into the primary health care system, decentralizing mental health care services to increase access to treatment and providing affordable service to maintain positive treatment outcomes.
Effect of Phosphorus and Zinc on the Growth, Nodulation and Yield of Soybean ...Premier Publishers
An investigation was carried out at Kogi State University Student Research and Demonstration farm Anyigba during the 2019 wet season to observe the effect of phosphorus and zinc on the growth, nodulation and yield of soybean. The treatments comprised three levels: phosphorus and zinc (0, 30 and 60 kg P2O5/ha; 0, 5 and 10kg Zn/ha) and two varieties TGX 536 – 02D and Samsoy 2. The investigation revealed that application of phosphorus affected growth, nodulation, yield and some yield components of soybean while zinc application, apart from the plant height, which is reduced significantly, had no significant effect on other growth characters, nodulation, yield and yield components. However, it was generally found to decrease most of the characters. Application of 60 kg P2O5/ha gave the highest growth and yield, while 30 kg P2O5/ha gave the highest nodulation. Application of 60 kg P2O5/ha significantly increased yield to 1.9t/ha, which was significantly higher over the control plots, which gave 1.7t/ha. Crude protein and oil contents of the seeds were not significantly affected by phosphorus application but were significantly affected by zinc application, which significantly decreased protein content as its amount an increase from 0 to 10 kg/ha, and significantly increased oil content from 0 to 5kg/ha and decreased it below 5kg/ha. It was also revealed that the two varieties responded similarly to phosphorus and zinc in terms of growth, grain yield and crude protein content of the seeds.
Influence of Harvest Stage on Yield and Yield Components of Orange Fleshed Sw...Premier Publishers
A field experiment was conducted at Adami Tullu Agricultural Research Center in 2018 under rainfed condition with supplementary irrigation to determine the influence of harvest stage on vine yield and tuberous root yield of orange fleshed sweet potato varieties. The experiment consisted of four harvest stages (105, 120, 135 and 150 days after planting) and Kulfo, Tulla and Guntute varieties. A 4 X 3 factorial experiment arranged in randomized complete block design with three replications was used. Interaction of harvest stage and variety significantly influenced above ground fresh biomass, vine length, marketable tuberous root weight per hectare, commercial harvest index and harvest index. The highest mean values of above ground fresh biomass (66.12 t/ha) and marketable tuberous root weight (56.39 t/ha) were produced by Guntute variety harvested at 135 days after planting. Based on the results, it can be recommended that, farmers of the study area can grow Guntute variety by harvesting at 135 days after planting to obtain optimum vine and tuberous root yields.
Performance evaluation of upland rice (Oryza sativa L.) and variability study...Premier Publishers
This study aimed at assessing genetic variability and to evaluate the performance of 13 improved upland rice varieties for yield and its components based on morphological traits. The field experiment was conducted using a randomized block design at Guraferda and Gimbo districts in the 2019 main cropping season. The analysis of variance (ANOVA) over the two locations revealed significant differences (p≤ 0.05) among varieties for days to 50% heading, days to 85% maturity, panicle length, thousand-grain weight, and grain yield. Similarly, the ANOVA for variety by location interactions depicted significant differences among the tested varieties for days to 50% heading, days to 85% maturity, and thousand-grain weight. High heritability was obtained from days to heading (88.5%), panicle length (85.0%), and grain yield (85.2%), which indicates these traits can be easily improved through selection. High to medium broad sense heritability and genetic advance as percentage of the mean for days to heading, thousand-grain weight, and grain yield indicates a good opportunity for improvement through selection using their phenotypic performance. This is mainly due to the high role of additive gene action in the expression of such traits. This study confirmed the presence of variability among varieties for most of the studied traits, which will create an opportunity for breeders to improve rice yield and other attributes.
Response of Hot Pepper (Capsicum Annuum L.) to Deficit Irrigation in Bennatse...Premier Publishers
This study was conducted at Enchete kebele in Benna-Tsemay Woreda, South Omo Zone to evaluate the response of hot pepper to deficit irrigation on yield and water productivity under furrow irrigation system. The experiment comprised four treatments (100 % of ETc, 85% of ETc, 70 % of ETc and 50% of ETc), respectively. The experiment was laid out in RCBD and replicated four times. The two years combined yield results indicated that, the maximum total yield (20.38 t/ha) was obtained from 100% ETc while minimum yield (12.92 t/ha) was obtained from 50% of ETc deficit irrigation level. The highest WUE 5.22 kg/ha mm-1 was obtained from 50% of ETc. Treatment of 100% ETc irrigation application had highest benefit cost ratio (4.5) than all others treatments. Applying 50% of ETc reduce the yield by 37% when compared to 100 % ETc. Accordingly, to achieve maximum hot pepper yield in areas where water is not scarce, applying 100% ETc irrigation water application level throughout whole growing season under furrow irrigation system is recommended. But, in the study area water scarcity is the major limiting factor for crop production. So, it is possible to get better yield and water productivity of hot pepper when we apply 85% ETc irrigation water throughout growing season under furrow irrigation system.
Harnessing the Power of Agricultural Waste: A Study of Sabo Market, Ikorodu, ...Premier Publishers
Nigeria is still burdened with huge responsibilities of waste disposal because the potential for benefits of proper waste management is yet to be harnessed. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. It is estimated that about 998 million tons of agricultural waste is produced yearly in the country with organic wastes amounting to 80 percent of the total solid wastes. This can be categorized into biodegradable and non-biodegradable wastes. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. The Sabo market was treated as a study case with the adoption of in-depth examinations of the facility, animals and products for sale and waste generated. A combination of experimental, interviews (qualitative) and design simulation (for final phase) was adopted to extract, verify and analyse the data generated from the study. Animal waste samples were subjected to compositional and fibre analysis with results showing that the sample has high potency for biogas production. Biodegradable Wastes are human and animal excreta, agricultural and all degradable wastes. Availability of high quantity of waste generated being organic in Sabo market allows the use of anaerobic digestion to be proposed as a waste to energy technology due to its feasibility for conversion of moist biodegradable wastes into biogas. The study found that at peak supply period during the Islamic festivities, a conservative 300tonnes of animal waste is generated during the week which translates to over 800kilowatts of electricity.
Influence of Conferences and Job Rotation on Job Productivity of Library Staf...Premier Publishers
The general purpose of this study is to investigate the influence of conferences and job rotation on job productivity of library staff in tertiary institutions in Imo State, Nigeria. The survey research design was used for this study using questionnaire as an instrument for data collection. This study covered the entire population of 661. Out of these, 501 copies of the questionnaire representing 75.8% were duly completed and returned for analysis. Student’s t-test was used to analyze the research questions. The finding showed that conferences had no significant influence on the job productivity of library staff in tertiary institutions in Imo State, Nigeria (F cal= 7.86; t-vale =6.177; p >0.005). Finding also showed that job rotation significantly influences job productivity of library staff in tertiary institutions in Imo State, Nigeria (F-cal value= 18.65; t-value = 16.225; P<0.05). This study recommended that, government should ensure that library staff participate in conferences with themes and topics that are relevant to the job they perform and also ensure that there should be proper evaluation and feedback mechanism which aimed to ensuring control and minimize abuse of their development opportunities. Again, there should be written statement of objectives in order to sustain job rotation programmes. Also, that training and development needs of library staff must be identified and analyzed before embarking on job rotation processes as this would help to build skills, competences, specialization and high job productivity.
Scanning Electron Microscopic Structure and Composition of Urinary Calculi of...Premier Publishers
Microscopic examination of urine samples collected from geriatric dogs revealed increased numbers of erythrocytes, leucocytes, epithelial cells and pus cells along with casts, bacteria, spermatozoa and crystals of various shapes. Among the different crystals, triple phosphate or struvite were predominant, followed by calcium oxalate dihydrate, calcium oxalate monohydrate and ammonium urate or biurate. The struvite crystals were, coffin-lid shape and while calcium oxalate dihydrate were octahedron or envelope and monohydrate crystals demonstrated “picket fence” and “dumbbell” and “hemp seed” appearance. Brown or yellow-brown spherical bodies with irregular borders with thorn-apple appearance were shown by ammonium urate or biurate crystals. SEM aspects of magnesium ammonium phosphate crystals revealed perpendicular columnar strata, few with scattered hexa or octa-hedral coffin-lid shaped crystals and calcium phosphate crystals were like cracked eggshells. Presence of wavy phases with sundry areas (uric acid), picket fence (calcium oxalate monohydrate) and typical envelope (calcium oxalate dehydrate) were electron microscopic appearance of various crystals.
Gentrification and its Effects on Minority Communities – A Comparative Case S...Premier Publishers
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The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
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Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
2. In vitro combine interactions of antimicrobial agents with plant extract against multidrug-resistant bacterial strains
Uzoechi et al. 013
effects of certain antibiotics, as well as the increasing
development of resistant to the antibiotics in current
clinical use (Cowan, 1999). Therefore, actions must be
taken to control the use of antibiotics, to better understand
the genetic mechanisms of resistance and to continue
studies to develop new drugs.
There are different approaches to cure and control the
infections caused by MDR bacterial strains. One of which
is by isolation of active phytochemicals that can help to
prevent the spread of infection. Another method is to
formulate new synergistic combination using different
commercially available antibiotics, or to combine an
antibiotic with active phytochemicals having antimicrobial
properties. It has been found that some medicinal plants
produce MDR inhibitors which enhance the activities of
antibiotics against MDR bacterial strains (Stermitz et al.,
2000).
Several in vitro studies have reported synergistic effects
resulting from the combination of different antibiotics with
different plants extracts against MDR bacterial strains
(Betoni et al., 2006). According to Estimone et al. (2006)
the herb-drug interaction between tea extract and
penicillin G against Staphylococcus aureus showed that
the interaction was mainly additive. Sibanda and Ocoh
(2008) showed potential of synergy between acetone
extracts of Garcinia kola and Amoxicillin, Ciprofloxacin,
Tetracycline and Chloramphenicol against MDR
pathogenic microorganisms.
Adiantum capillus-veneris is commonly known as maiden
hair fern belonging to kingdom Plantae and family
Adiantaceae, distributed throughout the world and in
different areas of Nigeria. It is traditionally used to treat
infectious diseases (Singh et al., 2008). It can be used as
expectorant, diuretic, as hair tonic and in chest disease
(Piyali et al., 2005). It has various phytochemicals like
alkaloids, tannins, steroids, flavonoids, saponins,
terpenoids, cardiac glycosides and reducing sugar, and
also have antibacterial and antifungal activity (Ishaq et al.,
2014).
Adiantum capillus-veneris has many traditional uses
globally. It is used for blood cleaning, cough, menstrual
and respiratory problems in Arizona. In Brazil it is used for
asthma, bronchitis, cough, digestion, excessive mucous,
flu and hair loss. Likewise, in India, it is used for asthma,
boils, bronchial disease and cold diabetes (Ansari and
Ekhlasi, 2012).
Therefore, the present study was designed to investigate
the antibacterial activity of methanolic extract of A.
capillus-veneris and their interaction with ten commonly
used antibiotics against MDR bacterial strains isolated
from clinical samples.
MATERIALS AND METHODS
Plant material collection and extraction
The A. capillus-veneris was collected from different areas
of Abajah in Nwangele LGA Imo State. The plant was
identified in the department of Botany, Imo State
university, while processed in microbiology laboratory.
The roots of A. capillus-veneris are used as plant sample
for extract preparation which were first thoroughly washed
with water and soaked in detergent to remove the
microbial load on the surface of plant sample. These were
then shade dried and ground to homogenous powder
(Sood and Sharma, 2010). One hundred gram of powder
was soaked in flask containing 1-liter n-hexane for 24 h at
25 °C and then filtered through Whatman No. 1 filter paper
(Cseke et al., 2006). The filtrate was collected in separate
flask and the same process was repeated for three times.
The filtrates, that is, crude extract obtained was
concentrated in rotary evaporator. For the isolation of pure
extract, the isolated crude extract was resuspended in a
minimum required volume of corresponding solvent and
placed on the water bath (60 °C) for the evaporation of
extra solvents. The extract was then preserved in separate
containers for further experimentations at 5 °C, according
to previous method of Deveeka et al. (2013).
Collection and identification of bacterial strains
Ten different bacterial strains used in this study were
isolated from different clinical samples like urine, wound
pus, and blood which were collected from the main
laboratory of Imo State University Teaching Hospital, St
John;s. Laboratory Owerri and University of Benin
teaching Hospital. The isolated bacterial strains were sub-
cultured on differential and selective media e.g.
MacConkey, SS agar, MSA and CLED agar and were
identified by their specific morphological and biochemical
characteristics (Collee and Marr, 1996).
Assessment of drug resistance pattern of test
bacterial strains
The Kirby-Baur method described by Benson (2002) was
used to study antimicrobial sensitivity tests. Disc diffusion
method was used for measurement of the antimicrobial
activity of antibiotic discs on Mueller-Hinton agar. The
organisms were tested against ten commonly used
antibiotics. Antibiotics used were Oxacillin (OX) (1µg),
Ceftazimide (CAZ) (30 µg), Cefotaxime (CTX) (30 µg),
Ceftriaxone (CRO) (30 µg), Cefuroxime (CXM) (30 µg),
Meropenem (MEM) (10 µg), Oflaxacin (OFX) (5 µg),
Cefoxitin (FOX) (30 µg), Ampicillin (AMP) (10 µg) and
Erythromycin (E) (15 µg) and the process was repeated
thrice. All the plates were incubated at 37 °C for 24 h.
3. In vitro combine interactions of antimicrobial agents with plant extract against multidrug-resistant bacterial strains
World Res. J. Biol. Biol. Sci. 014
Antimicrobial activity of plant extract
Well diffusion method of Janovska et al. (2003) was
followed with some modifications for the antimicrobial
activity of plant extracts. One mg of plant extract was
dissolved in 1 mL of dimethyl sulfoxide (DMSO) (1 mg/1
mL). Pre-autoclaved Nutrient agar plates were inoculated
with a 10ˉ5 dilution of bacterial culture, using sterile cotton
swabs to achieve uniform lawn of growth and sterile corn
borer was used to bore wells in the agar. Sixty microliters
of extract were introduced through micropipette aseptically
into specifically marked wells in the agar plates. All
cultured plates were incubated at 37 °C for 24 h and the
process was repeated thrice.
Combined plant extract and antibiotics
The combine activity of plant extract and antibiotics was
calculated by combining different antibiotics and extract
using agar well method. The plates were incubated for 24
h at 37 °C and synergistic and antagonistic effects were
measured and compared with individually used extract and
antibiotics (Okore, 2009).
RESULTS
Drug resistance profile of the test bacterial strains
Different bacterial species were isolated from different
sources like S. aureus and Salmonella typhi were isolated
from blood samples, Escherichia coli, Klebsiella
pneumoniae, Citrobacter freundii, Shigella dysenteriae,
Proteus vulgaris and Providencia species from urine while
Pseudomonas aeruginosa and Staphylococcus
epidermidis were isolated from wound pus. The bacterial
strains were tested for antibiotics sensitivity profile against
10 frequently used antibiotics. Most of the tested bacterial
strains were found to be resistant to the used antibiotics.
E. coli and S. typhi were found to be the most resistant
bacterial strains (60%) to all tested antibiotics, followed by
S. dysenteriae, K. pneumoniae and Providencia (50%), S.
aureas and P. aeruginosa (40%), C. freundii (30%), S.
epidermidis and P. vulgaris (Figure 1).
Figure 1: Percentage of antibiotic sensitivity of MDR bacterial strains. Amongst all used antibiotics MEM showed
maximum effect against all tested bacterial strains. The maximum ZI was shown by Providencia species and P. aeruginosa
(34 mm) followed by E. coli and S. dysentreiae (33 mm), P. vulgaris (32.5 mm), C. freundii (32 mm), S. aureus and S.
epidermidis (31 mm), S. typhi (30 mm), and K. pneumoniae (25 mm). The most effective antibiotics were OFX, CTX and
CRO (Figure 1 and Table 1).
0 10 20 30 40 50 60
E. coli
S. typhi
S. dysentriae
K. pneumoniea
Providencia
S.aureus
P.aerogenosa
C.fruendii
S.epidermidis
P.vulgaris
Percentage of antibiotic sensitivity of MDR bacterial strains
4. In vitro combine interactions of antimicrobial agents with plant extract against multidrug-resistant bacterial strains
Uzoechi et al. 015
Table 1: Drug resistance profile of test bacterial strains.
Antibiotics disc with ZI (mm), while (--) representing resistance
Microorganisms 1 2 3 4 5 6 7 8 9 10
OX CAZ CTX CXM MEM FOX OFX AMP E CRO
E. coli -- -- 18 -- 33 20.5 -- -- -- 17.5
S. epidermidis -- -- 18 12 31 26.5 18 12.5 22 15.5
S. dysenteriae -- 22 17 -- 33 23 -- -- -- 18
S. typhi -- -- 13 -- 30.5 18.5 -- -- -- 8
P. vulgaris -- -- 19 15 31.5 30 18.5 14 25 19
Providencia -- 17 16 -- 34 10 -- -- -- 20
S. aureus -- 24 14.5 -- 31 20 -- 8 -- 15
P. aeruginosa -- 14 15 -- 34 19.5 12 -- -- 20
K. pneumoniae -- 10 -- -- 25 20 11 -- 8 --
C. fruendii -- 28 18 -- 32 18 -- -- -- 18
Table 3: Combined activities of antibiotics with methanolic extract of A. capillus-veneris.
Microorganisms
Combined ZI in (mm) of plant extract with antibiotics
1 2 3 4 5 6 7 8 9 10
OX CAZ CTX CXM MEM FOX OFX AMP E CRO
E. coli 12s --i 22s 23s 38s 21s --i 8s --i 20s
S. epidermidis 15s 20s 21s 16s 40s 24a 22 ND ND 22s
S. dysenteriae --i 26s 20s 15s 38s 25s ND ND ND 22s
S. typhi --i 42s 17s 10s 35s 23s ND ND ND 28s
P. vulgaris --i 32s 17a 30s 40s --a 30s 16s 25s 20s
Providencia --i 22s 18s 10s 35s 18s 35s ND ND 23s
S. aureus 14s 31s 25s 17s 40s 25s ND ND ND 24s
P. aeruginosa --i 20s 22s 16s 40s 16a 20s ND ND 22s
K. pneumoniae --i --i 15s --i 33s 16a 18s ND ND 20s
C. fruendii --i 32s 18s --i 35s 15s ND ND ND 25s
--, No ZI;I, Indifferent; s, Synergistic effect; a, Antagonistic effect; ND, Not done.
Assessment of antibacterial activity of plant extract
The extracts of Adiantum capillus-veneris showed little
effect against tested bacterial strains. The extract showed
maximum ZI against E. coli (16 mm), followed by S.
epidermidis and C. freundii (12 mm), S. dysenteriae and
S. aureas (10 mm), Providencia species (8 mm), P.
aeruginosa (7 mm), while showed resistance to S. typhi ,
P. vulgaris and K. pneumoniae (Table 2).
Table 2: Antibacterial activity of plant extract.
Microorganisms
Antibacterial activity of extract of
Adiantum capillus-veneris against
MDR bacterial strains
E. coli 16
S. epidermidis 12
S. dysenteriae 10
S. typhi 0
P. vulgaris 0
Providencia species 8
S. aureus 10
P. aeruginosa 7
K. pneumoniae 0
C. freundii 11
Extracts with zone of inhibition (ZI) representing sensitivity
in millimeter (mm)
Combined effect of plant extract with antibiotics
The combined effects of plant extract with antibiotics were
different from individually used antibiotics and extract of
Adiantum capillus-veneris. The maximum antibiotics
showed synergistic effects, some were antagonistic while
some of them showed neither synergistic nor antagonistic
effects against test MDR bacterial strain. CXM combined
with mEXT showed synergistic effects against most of the
bacterial strain. Its Maximum ZI was against P. vulgaris (30
mm), S. aureus (17 mm), P. aeruginosa and S. epidermidis
(16 mm), S. dysenteriae (15 mm), S. typhi and Providencia
species (10 mm), while there were no significant effects
against K. pneumoniae and C. freundii. Another antibiotic
CTX showed good effect in combination with plant extract
against all tested organisms. The maximum combined ZI
of CTX with plant extract was observed against S. aureus
(25 mm), followed by E. coli and P. aeruginosa (22 mm),
S. dysenteriae and S. epidermidis (21mm) Providensia
species and C. freundii (18 mm) S. typhi and P. vulgaris
(17 mm) and K. pneumoniae (15 mm). Other antibiotics
that were used in combination with mEXT are shown in
Table 3.
5. In vitro combine interactions of antimicrobial agents with plant extract against multidrug-resistant bacterial strains
World Res. J. Biol. Biol. Sci. 016
DISCUSSION
The outgoing emergence of antibiotic resistance has
diverted the attention of researchers towards the medicinal
plants and search of new and effective drugs. In this
connection, Yang et al. (2010) stated that, in recent years,
human pathogenic microorganisms have developed
multiple drug resistance and causing nosocomial
infections. Moreover, it was suggested that plant extracts
can be further developed into antibiotic medicine due to
their proven antimicrobial activity.
The present study has shown that methanolic extract of
roots of A. capillus-veneris in combination with antibiotics
inhibited the growth of test MDR bacterial strains at high
level than were tested separately. This effect was
synergistic or additive for the most of the test bacterial
strains. Synergistic and additive interactions are a result of
a combined effect of active compounds from plant extract
and antibiotics. The plant extracts contain a great number
of different compounds (phenol. flavonoids, tannins,
coumarins, alkaloids and terpenoids) which have an
impact on growth and metabolism of microorganisms
(Cowan, 1999).
In the present study 10 bacterial strains were used which
were multidrug-resistant to most of the given antibiotics.
The results showed that E. coli and S. typhi were the most
resistant strains (60%) among all the tested bacterial
strains. This result goes in line with the studies in other
countries of the world where 95-100% MDR E. coli was
reported (Dimah, 2012). Moreover, (67%) MDR E.coli was
seen in the study of Ogunleye et al. (2008). In India 10%
MDR S. typhi was reported (Nagshetty et al., 2010).
Similarly, 50% MDR Providencia species evaluated in our
study is almost in line with the study of Tumbarello et al.
(2004) who reported 75% MDR Providencia species. We
have also investigated that S. dysenteriae, S. aureus,
P.aeruginosa, C. freundii, S. epidermidis and P. vulgaris
are rather more MDR than what was found in other regions
of the world.
Several studies on A. capillus-veneris revealed its potency
against MDR bacterial strains. For example E.coli, S.
epidermidis, S. dysenteriae, Providencia species, P.
aeruginosa and C. freundii were sensitive to methanolic
extract of root of A. capillus-veneris, while K. pneumoniae,
S. typhi and P. vulgaris were resistant. This study proved
to be almost in accordance with the finding of Kumar and
Nagarjan (2012) and Mahboubi et al. (2012).
Our result revealed that the combination of plant extracts
and antibiotics could be useful in treatment of infectious
diseases and useful in fighting emerging drug resistance
problems. Synergistic effect was more pronounced in case
of all the strains as evident from zone of inhibition of all the
strains presented in Table 3. In all cases more than 15%
increase in ZI was observed which is highly significant.
Several studies evaluated the interactions of plants extract
and certain antimicrobial agents and their impacts on MDR
bacterial strains. Tiwari et al. (2005) reported that
Chloramphenicol and Tea extract in combination inhibited
the growth of S. dysenteriae, tea extract showed
synergistic activity with Chloramphenicol and other
antibiotics like Gentamycin, Methicillin and Nalidixic acid
against tested bacterial strains. Voukeng et al. (2012)
indicated that plant extracts contain chemicals that can
modulate the activity of antibiotics against bacteria
expressing MDR phenotypes.
Comparing with all these studies, the study concluded that
the extract of roots of A. capillus-veneris have bioactive
compounds that enhances the antimicrobial activities of
antibiotics that have shown no effect or little effect against
MDR bacterial strains.
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Benson, J. H. (2002). Microbiological applications:
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