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GOLDEN RICE Vitamin A enriched transgenic Rice
What is golden Rice? ,[object Object],[object Object],[object Object]
Need for GR ,[object Object],[object Object],[object Object],[object Object],[object Object]
VAD in women ,[object Object],[object Object],[object Object]
Combating VAD ,[object Object],Fungi γ  carotene Tomatoes δ  carotene Green leafy veg,  many fruits and sea weeds β  carotene  Carrots, palm oil α  carotene Plant sources Common carotenoids
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Development of Golden Rice ,[object Object],[object Object],[object Object]
Inventors
Daffodils Gene transfer Golden Rice Erwinia uredovora Gene Sources
Genes transferred Bacteria ( Erwinia uredovora ) Phytoene desaturase ctrl Daffodils Lycopene  β  cyclase   icy Daffodils Phytoene synthase psy Source Enzyme coded Gene
Beta Carotene Biosynthetic Pathway  GGDP Tocopherols Gibberellins Chlorophyll Phytoene Phytoene synthase (psy) Phytoene desaturase (ctrl) Lycopene Lycopene  β  cyclase (icy) β   Carotene
pB19hpc (Golden Rice Cassette) T L T R aphIV 35S Gt1 psy 35S rbcS crtl Hygromycin Resistance Phytoene Synthase Phytoene Desaturase T-DNA Border T-DNA Border Selectable Marker Gene of Interest Gene of Interest Insertion Sequence Insertion Sequence
Difficulties ,[object Object],[object Object],[object Object],[object Object]
Mile Stones ,[object Object],[object Object],[object Object],[object Object],Golden Rice (Potrykus  et al  2000)
Testing the transformants ,[object Object],[object Object],[object Object]
Accomplishments ,[object Object],[object Object],[object Object]
Benefits of G. Rice ,[object Object],[object Object],[object Object],[object Object],[object Object]
Controversies ,[object Object],[object Object],[object Object],[object Object]
Challenges ahead ,[object Object],[object Object],[object Object],[object Object],[object Object]
Golden Rice deserves a chance
[object Object]

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Golden rice

  • 1. GOLDEN RICE Vitamin A enriched transgenic Rice
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 9. Daffodils Gene transfer Golden Rice Erwinia uredovora Gene Sources
  • 10. Genes transferred Bacteria ( Erwinia uredovora ) Phytoene desaturase ctrl Daffodils Lycopene β cyclase icy Daffodils Phytoene synthase psy Source Enzyme coded Gene
  • 11. Beta Carotene Biosynthetic Pathway GGDP Tocopherols Gibberellins Chlorophyll Phytoene Phytoene synthase (psy) Phytoene desaturase (ctrl) Lycopene Lycopene β cyclase (icy) β Carotene
  • 12. pB19hpc (Golden Rice Cassette) T L T R aphIV 35S Gt1 psy 35S rbcS crtl Hygromycin Resistance Phytoene Synthase Phytoene Desaturase T-DNA Border T-DNA Border Selectable Marker Gene of Interest Gene of Interest Insertion Sequence Insertion Sequence
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 21.

Editor's Notes

  1. This slide demonstrates one of the transformation cassettes used to develop “Golden Rice” was developed. Slowly click through this slide, and you will see each of the components of the cassette.