Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

1. SHRI SHANKARACHARYA
MAHAVIDAYALAYA
JUNWANI ,BHILAI
AGAROSE GEL ELECTROPHORESIS
Dr.Rachana choudhary
Astt.Professor-

2. SYNOPSIS
 INTRODUCTION
 HISTORY
 PRINCIPLE
 FACTORS AFFECTING ELECTROPHORESIS
 TYPES OF ELECROPHORESIS
 AGAROSE GEL ELECTROPHORESIS
 PROCEDURE
 APPLICATION
 CONCLUSION
 REFERENCE

3. WHAT IS ELECTROPHORESIS?
 It is the migration of charged particles in a
medium under the influence of applied
electric field.
PURPOSE OF ELECTROPHORESIS
 To determine the number amount & mobility
of components in given sample.
HISTORY
It is first developed by A.Tiselius.

5. FACTORS AFFECTING ELECTROPHORESIS
MOBILITY
 THE SAMPLE- Charge,Mass Shape.
 ELECTRIC FIELD-The temperature with in the
electrophoresis cell should be controlled.
 THE MEDIUM-Inert supporting medium’s chosen.
1. Adsorption
2. Molecular sieving
 THE BUFFER- It should be chosen depending
upon -
1. Composition
2. Ionic strength
3. pH

6. TYPES OF ELECTROPHORESIS
1. FREE ELECTROPHORESIS
Microelectrophoresis
Moving boundary electrophoresis
2. ZONAL ELECTROPHORESIS
Paper electrophoresis
Gel electrophoresis

10. AGAROSE

21. APPLICATION
 It is used for isolation of DNA.
 Determining the size of DNA
molecule.
 Used for DNA finger printing.

22. CONCLUSION
 From the above discussion it is concluded
that agarose gel electrophoresis is the most
commonly used technique for seperation and
quantitative estimation of biomolecules like
RNA and DNA.

23. REFERENCE
 INSTRUMENTATION BY –UPADHYAYA and
UPADHYAYA.
 INSTRUMENTATION BY- CHATWAL and
CHATWAL.
 INSTRUMENTATION BY-WILSON and
WALLKER.

24. THANKS