Electrophoresis
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In this slide, lil bit info regards electrophoresis while a complete general operation of gel electrophoresis.
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Electrophoresis
it is a short ppt. on Electrophoresis. Which gives it's defination, Types of electrophoresis ; Like:- Zone electrophoresis, Isoelectric focussing, Immunoelectrophoresis. and their images.
ELECTROPHORESIS AND ITS TYPES
Paper electrophoresis
gel electrophoresis
capillary electrophoresis
zone electrophoresis
moving boundary electrophoresis
iso electric focusing electrophoresis
Electrophoresis, Types of electrophoresis
Electrophoresis, Types of electrophoresis, zone ectrophoresis, capillary ectrophoresis, gel ectrophoresis, free ectrophoresis.
Gel electrophoresis
Gel electrophoresis is a technique used to separate biomolecules like DNA, RNA, and proteins based on their size and charge. It works by applying an electric current to a gel which causes the charged molecules to migrate at different rates depending on factors like their mass and shape. There are two main types of gels used - agarose gels are used for separating larger molecules like nucleic acids, while polyacrylamide gels can provide better resolution for smaller molecules like proteins. Gel electrophoresis has many applications, including determining molecular weights, analyzing protein subunits, monitoring changes in body fluids, and separating components in a sample.
Electrophoresis
What is Electrophoresis?
Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
This electrokinetic phenomenon was first observed in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.
Electrophoresis of positively charged particles (cations) is called cataphoresis while electrophoresis of negatively charged particles (anions) is called anaphoresis.
Electrophoresis , principles and application
Capillary electrophoresis is a separation technique where electrophoresis is performed inside narrow capillaries. Charged molecules or ions migrate through the capillary under the influence of an electric field. The rate of migration depends on factors like the molecule's net charge, size, shape, and the electric field strength. There are two main types - capillary zone electrophoresis, which separates analytes in buffer alone, and capillary gel electrophoresis, which uses a gel matrix to separate based on size. Capillary electrophoresis has applications in fields like pharmaceuticals, forensics, foods, and biosciences for analyzing substances like DNA, proteins, metals, and organic compounds.
Electrophoresis presentation
1. Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids by using an electric field to move them through a medium like gel or paper.
2. The rate at which molecules move depends on factors like their charge, size, and shape. Larger or more irregularly shaped molecules move more slowly.
3. Common types of electrophoresis include paper electrophoresis, cellulose acetate electrophoresis, and gel electrophoresis using agarose or polyacrylamide gels. Gel electrophoresis is often used to separate proteins and DNA fragments by size.
Electrophoresis
Electrophoresis is a technique used to separate charged particles such as proteins, nucleic acids, and other macromolecules. It works by applying an electric field to move these particles through a medium such as a gel or paper based on their size and charge. There are different types of electrophoresis including gel electrophoresis, paper electrophoresis, capillary electrophoresis, and isoelectric focusing which separates particles based on their isoelectric point. Electrophoresis has many applications in fields like forensics, molecular biology, genetics, and biochemistry to analyze proteins, DNA, RNA, and other biomolecules.
Recommended
Electrophoresis
it is a short ppt. on Electrophoresis. Which gives it's defination, Types of electrophoresis ; Like:- Zone electrophoresis, Isoelectric focussing, Immunoelectrophoresis. and their images.
ELECTROPHORESIS AND ITS TYPES
Paper electrophoresis
gel electrophoresis
capillary electrophoresis
zone electrophoresis
moving boundary electrophoresis
iso electric focusing electrophoresis
Electrophoresis, Types of electrophoresis
Electrophoresis, Types of electrophoresis, zone ectrophoresis, capillary ectrophoresis, gel ectrophoresis, free ectrophoresis.
Gel electrophoresis
Gel electrophoresis is a technique used to separate biomolecules like DNA, RNA, and proteins based on their size and charge. It works by applying an electric current to a gel which causes the charged molecules to migrate at different rates depending on factors like their mass and shape. There are two main types of gels used - agarose gels are used for separating larger molecules like nucleic acids, while polyacrylamide gels can provide better resolution for smaller molecules like proteins. Gel electrophoresis has many applications, including determining molecular weights, analyzing protein subunits, monitoring changes in body fluids, and separating components in a sample.
Electrophoresis
What is Electrophoresis?
Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
This electrokinetic phenomenon was first observed in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.
Electrophoresis of positively charged particles (cations) is called cataphoresis while electrophoresis of negatively charged particles (anions) is called anaphoresis.
Electrophoresis , principles and application
Capillary electrophoresis is a separation technique where electrophoresis is performed inside narrow capillaries. Charged molecules or ions migrate through the capillary under the influence of an electric field. The rate of migration depends on factors like the molecule's net charge, size, shape, and the electric field strength. There are two main types - capillary zone electrophoresis, which separates analytes in buffer alone, and capillary gel electrophoresis, which uses a gel matrix to separate based on size. Capillary electrophoresis has applications in fields like pharmaceuticals, forensics, foods, and biosciences for analyzing substances like DNA, proteins, metals, and organic compounds.
Electrophoresis presentation
1. Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids by using an electric field to move them through a medium like gel or paper.
2. The rate at which molecules move depends on factors like their charge, size, and shape. Larger or more irregularly shaped molecules move more slowly.
3. Common types of electrophoresis include paper electrophoresis, cellulose acetate electrophoresis, and gel electrophoresis using agarose or polyacrylamide gels. Gel electrophoresis is often used to separate proteins and DNA fragments by size.
Electrophoresis
Electrophoresis is a technique used to separate charged particles such as proteins, nucleic acids, and other macromolecules. It works by applying an electric field to move these particles through a medium such as a gel or paper based on their size and charge. There are different types of electrophoresis including gel electrophoresis, paper electrophoresis, capillary electrophoresis, and isoelectric focusing which separates particles based on their isoelectric point. Electrophoresis has many applications in fields like forensics, molecular biology, genetics, and biochemistry to analyze proteins, DNA, RNA, and other biomolecules.
ELECTROPHORESIS
Electrophoresis is a technique used to separate charged particles like proteins by using an electric field to migrate them through a medium like filter paper or gel based on factors like their size and charge. It can be used to separate plasma proteins, lipoproteins, and immunoglobulins into distinct bands. The document discusses several electrophoresis techniques including paper, gel, SDS-PAGE, isoelectric focusing, and immunoelectrophoresis and how they are used to separate biomolecules.
Electrophoresis sem4 paper1 MITHIBAI COLLEGE
This document discusses electrophoresis, which separates molecules based on their charge and size. It describes the principles of electrophoresis, factors that affect separation, different support media used, techniques, detection methods, types of electrophoresis including zone electrophoresis and gel electrophoresis, applications, advantages, and disadvantages. Electrophoresis is used to analyze, identify, purify, and separate mixtures of charged biomolecules like proteins, nucleic acids, and other molecules.
Paper electrophoresis
The technique of paper electrophoresis is simple and inexpensive and requires only micro quantities of plasma for separation.
The support medium is a filter paper
The electrophoresis apparatus in its simplest form consists of two troughs to contain buffer solution, through which electric current is passed.
Frequently used in isolating proteins, amino acids and oligopeptides.
Electrophoresis
Gel electrophoresis is a method used to separate macromolecules like DNA, RNA, and proteins based on their size and charge. It uses a gel as a medium for separation under an electric field. Smaller molecules move faster through the gel towards the positive electrode. The separated molecules can then be visualized and identified. Gel electrophoresis is more efficient at separation than paper electrophoresis and allows for separation of a wider range of molecule sizes.
Electrophoresis
This document discusses electrophoresis, which is the movement of charged particles in an electric field. It separates molecules based on their charge and size. Key factors that affect migration rate are listed. The main requirements for electrophoresis are an electrophoresis tank, electrodes, power supply, buffer, and specimens like serum or plasma. Common electrophoresis techniques described include zone electrophoresis using paper or gel, isoelectric focusing, immuno electrophoresis, and SDS-PAGE which separates based on size. Clinical applications involve using electrophoresis to analyze conditions like liver disease or infections.
Electrophoresis, principle and types
This document discusses different types of electrophoresis techniques. It begins with an introduction to electrophoresis and its history. It then describes various electrophoresis techniques including gel electrophoresis using agarose gel or polyacrylamide gel, capillary electrophoresis, isoelectric focusing, two-dimensional electrophoresis, and immuno electrophoresis. The document explains the basic principles of electrophoresis and how it uses an applied electric field to separate biomolecules like proteins, nucleic acids, and carbohydrates based on their charge and size. It concludes with some applications of electrophoresis techniques.
Modern pharmaceutical analytical technique ( Electrophoresis)
This document provides an overview of electrophoresis, including definitions, principles, types, and applications. It discusses four main types of electrophoresis: paper electrophoresis, gel electrophoresis, capillary electrophoresis, and iso-electric electrophoresis. Paper and gel electrophoresis are described as zone electrophoresis techniques that separate components into bands on a supporting medium like paper or gel. Capillary electrophoresis allows separation in free solution within a thin capillary tube. Iso-electric electrophoresis separates molecules based on their iso-electric point, the pH where they have no net charge. The document outlines the principles, instrumentation, and applications of each electrophoresis technique.
Paper electrophoresis
Paper electrophoresis is a simple and inexpensive technique that uses filter paper and micro quantities of plasma to separate molecules like proteins, amino acids, and oligopeptides based on their charge. The basic equipment needed includes a power pack to provide a stabilized current and an electrophoretic cell containing electrodes, buffer reservoirs, and a support for the filter paper. Samples are applied to filter paper strips, which are placed in buffer solutions and an electric field is applied, causing charged particles to migrate and separate into distinct bands based on their size and charge. Paper electrophoresis is commonly used to analyze proteins in human plasma and other biological samples.
Electrophoresis
This document discusses electrophoresis, which is the migration of charged particles through a liquid medium under the influence of an electric field. It defines key terms and describes the theory behind electrophoresis, factors that influence particle migration rates, and different electrophoresis techniques. Some main techniques covered are agarose gel electrophoresis, polyacrylamide gel electrophoresis, isoelectric focusing, and two-dimensional electrophoresis. Troubleshooting tips for common issues are also provided.
Electrophoresispresentation
This document provides information about electrophoresis. It begins by defining electrophoresis as the migration of charged particles or molecules under the influence of an electric field. Some purposes of electrophoresis are to determine components in a sample, separate them, and obtain information about electrical double layers or determine molecular weights. The principle described is that charged particles migrate in an electric field at a velocity depending on factors like charge, size, shape and applied current. Different types of electrophoresis are described like paper, gel, and isolectric focusing electrophoresis. Key aspects of each technique including apparatus, sample application, detection, and preparation of gels are explained. Two-dimensional electrophoresis combining isolectric focusing and SDS-PAGE is also summarized.
electrophoresis
This document provides an overview of electrophoresis techniques. It begins with an introduction to electrophoresis as the migration of charged particles under an electric field. It then discusses various factors that affect electrophoresis like net charge, size, strength of electric field, buffer properties, and temperature. It describes different types of electrophoresis like paper, gel, cellulose acetate, isoelectric focusing, and moving boundary electrophoresis. Specific details are given about gel electrophoresis techniques like agarose, polyacrylamide gel, and SDS-PAGE electrophoresis. Other techniques like isolectric focusing, two-dimensional electrophoresis, isotachophoresis, pulsed field gel electrophoresis, and immunoelectrophoresis are also summarized briefly in 3 sentences
Electrophoresis
Electrophoresis is a method used to separate charged particles such as proteins and nucleic acids based on differences in their migration speed in an electric field. There are several types of electrophoresis including agarose gel electrophoresis, SDS-PAGE, isoelectric focusing, capillary electrophoresis, and two-dimensional electrophoresis. During electrophoresis, charged particles migrate through a gel or capillary towards an electrode with the rate of migration depending on factors like the particle's charge, size, and shape.
Electrophoresis
Electrophoresis is an analytical technique that separates and analyzes ionized analytes using their migration in an electric field. Charged molecules are separated as they migrate through a porous medium like agarose or polyacrylamide gel under the influence of an electric field generated between electrodes. The direction of migration depends on the charge, with positively charged ions or DNA fragments migrating toward the negative cathode and negatively charged molecules toward the positive anode. Different electrophoresis methods like agarose gel electrophoresis and polyacrylamide gel electrophoresis are used depending on the size of DNA fragments to be separated.
Basics of electrophoresis
Electrophoresis is a technique used to separate molecules based on their charge and size. It works by applying an electric field to move charged molecules through a medium such as a gel or paper. There are different types depending on the medium used, such as polyacrylamide gel electrophoresis and agarose gel electrophoresis. Electrophoresis has many applications including determining molecular weights, studying protein-protein interactions, and purifying proteins and DNA fragments.
gel electrophoresis by suresh b i
Gel electrophoresis is a method used to separate molecules like proteins and nucleic acids based on their physical properties. It works by applying an electric field to move charged molecules through a gel medium. Key aspects include applying samples to the gel, running electrophoresis with an electric current, and visualizing separated molecules by staining and quantification using a densitometer. Gel electrophoresis has applications in forensics, genetics, and biochemistry to analyze DNA, diagnose diseases, and study proteins. It separates biomolecules based on factors like size, shape, and charge.
Electrophoresis by Hina Ialsm Chishty
Electrophoresis is a technique used to separate charged molecules such as proteins and nucleic acids based on their charge and size ratios. It involves applying an electric field to migrate molecules through a medium like gel or paper. Initially developed in the 1930s to separate serum proteins, it has since been used for applications like human genome sequencing and disease diagnosis. Improvements to the technique include capillary electrophoresis using narrow tubes, isoelectric focusing to separate molecules at their isoelectric point, and immuno electrophoresis to study antibody-antigen reactions. A variety of detectors can identify separated molecules based on properties like UV absorption or electric resistance. Electrophoresis has applications in areas like forensics, clinical testing, and proteomics.
Electro phoresis sud mpharm pdf
The document provides an overview of electrophoresis, including:
1) Electrophoresis is a separation technique where charged molecules migrate at different rates in an electric field, allowing separation.
2) It is used to separate biological substances like proteins, nucleic acids, and amino acids.
3) There are different electrophoresis methods including capillary electrophoresis, which uses narrow capillaries, and zone electrophoresis using papers, gels, or thin layers to support molecules.
4) Factors like each molecule's charge, size, and the electric field strength determine their movement during electrophoresis.
Prabhakar singh ii sem-paper v-electrophoresis
Prabhakar singh ii sem-paper v-electrophoresisDepartment of Biochemistry, Veer Bahadur Singh Purvanchal Univarsity, Jaunpur
The document discusses electrophoresis, which is the movement of charged particles in an electric field. It migrates towards the oppositely charged electrode. Electrophoresis is used to separate biological molecules like proteins. It explains the principles and types of electrophoresis like paper, gel, and isolectric focusing electrophoresis. It provides details about agarose gel electrophoresis including properties, buffers used, procedure, and applications. It also briefly discusses polyacrylamide gel electrophoresis.Electrophoresis
Electrophoresis is an electrokinetic process which separates charged particles in a fluid using a field of electrical charge. It is most often used in life sciences to separate protein molecules or DNA and can be achieved through several different procedures depending on the type and size of the molecules. The procedures differ in some ways but all need a source for the electrical charge, a support medium and a buffer solution. Electrophoresis is used in laboratories for the separation of molecules based on size, density and purity.An electric field is applied to molecules and as they are electrically charged themselves it results in a force acting upon them. The greater the charge of the molecule the greater the force applied by the electrical field and therefore the further through the support medium the molecule will move relative to its mass.
Some example applications of electrophoresis include DNA and RNA analysis as well as protein electrophoresis which is a medical procedure used to analyse and separate the molecules found in a fluid sample (most commonly blood and urine samples).Different types of gels are usually used as the support medium for electrophoresis and this may be in slab or tube form depending on which is more beneficial. Gel slabs enable many samples to be run simultaneously and so are frequently used in laboratories. However, tube gels give a better resolution of the results so are often chosen for protein electrophoresis.
Agarose gel is commonly used for electrophoresis of DNA. It has a large pore structure allowing larger molecules to move easily but it is not suitable for sequencing smaller molecules.
Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose gel making it more suitable for quantitative analysis. This makes it possible to identify how proteins bind to DNA. It can also be used to develop an understanding of how bacteria is becoming resistant to antibiotics through plasmid analysis.
Electrophoresis
INTRODUCTION, DEFINATION OF ELECTROPHORESIS, ELECTROPHORESIS PRINCIPLE, TYPES OF ELECTROPHORESIS, FREE ELECTROPHORESIS, ZONE ELECTROPHORESIS,PAPER ELECTROPHORESIS, WORKING OF PAPER ELECTROPHORESIS, PROCEDURE FOR PAPER ELECTROPHORESIS, VISUALISATION, FACTORS AFFECTING SEPARATION OF MOLECULES, APPLICATIONS, working of paper electrophoresis ,procedure for paper electrophoresis ,visualisation ,factors affecting separation of molecules ,applications ,forensics ,dna fingerprinting ,molecular biology ,microbiology information about the organisms ,biochemistry mapping of cellular components ,paper electrophoresis is also used in study of sic ,hemoglobin abnormalities ,separation of blood clotting factors ,serum plasma proteins from blood sample ,used in separation and identification of alkaloids ,used for testing water samples ,toxicity of water ,drug industry to determine presence of illelgal drUGS
ELECTROPHORESIS AND ITS FORENSIC APPLICATIONS.pptx
Electrophoresis is a technique used to separate charged particles such as proteins, nucleic acids, and other molecules using an electric field. It was first observed in 1807 and involves applying an electric field to cause charged particles in solution to migrate toward the electrode of opposite charge. There are several types of electrophoresis including paper, gel, and capillary electrophoresis. Electrophoresis is widely used in forensic science applications such as DNA fingerprinting to identify suspects by comparing DNA profiles.
electrophorosis (1).pptx
This presentation discusses various types of electrophoresis, including paper electrophoresis, gel electrophoresis, capillary electrophoresis, and moving boundary electrophoresis. It explains the basic principles of electrophoresis, which involves the separation of charged particles in a conducting medium under the influence of an electric field. Factors that affect electrophoresis like charge, size, and pH are also summarized. The instrumentation and applications of each type of electrophoresis are briefly described.
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ELECTROPHORESIS
Electrophoresis is a technique used to separate charged particles like proteins by using an electric field to migrate them through a medium like filter paper or gel based on factors like their size and charge. It can be used to separate plasma proteins, lipoproteins, and immunoglobulins into distinct bands. The document discusses several electrophoresis techniques including paper, gel, SDS-PAGE, isoelectric focusing, and immunoelectrophoresis and how they are used to separate biomolecules.
Electrophoresis sem4 paper1 MITHIBAI COLLEGE
This document discusses electrophoresis, which separates molecules based on their charge and size. It describes the principles of electrophoresis, factors that affect separation, different support media used, techniques, detection methods, types of electrophoresis including zone electrophoresis and gel electrophoresis, applications, advantages, and disadvantages. Electrophoresis is used to analyze, identify, purify, and separate mixtures of charged biomolecules like proteins, nucleic acids, and other molecules.
Paper electrophoresis
The technique of paper electrophoresis is simple and inexpensive and requires only micro quantities of plasma for separation.
The support medium is a filter paper
The electrophoresis apparatus in its simplest form consists of two troughs to contain buffer solution, through which electric current is passed.
Frequently used in isolating proteins, amino acids and oligopeptides.
Electrophoresis
Gel electrophoresis is a method used to separate macromolecules like DNA, RNA, and proteins based on their size and charge. It uses a gel as a medium for separation under an electric field. Smaller molecules move faster through the gel towards the positive electrode. The separated molecules can then be visualized and identified. Gel electrophoresis is more efficient at separation than paper electrophoresis and allows for separation of a wider range of molecule sizes.
Electrophoresis
This document discusses electrophoresis, which is the movement of charged particles in an electric field. It separates molecules based on their charge and size. Key factors that affect migration rate are listed. The main requirements for electrophoresis are an electrophoresis tank, electrodes, power supply, buffer, and specimens like serum or plasma. Common electrophoresis techniques described include zone electrophoresis using paper or gel, isoelectric focusing, immuno electrophoresis, and SDS-PAGE which separates based on size. Clinical applications involve using electrophoresis to analyze conditions like liver disease or infections.
Electrophoresis, principle and types
This document discusses different types of electrophoresis techniques. It begins with an introduction to electrophoresis and its history. It then describes various electrophoresis techniques including gel electrophoresis using agarose gel or polyacrylamide gel, capillary electrophoresis, isoelectric focusing, two-dimensional electrophoresis, and immuno electrophoresis. The document explains the basic principles of electrophoresis and how it uses an applied electric field to separate biomolecules like proteins, nucleic acids, and carbohydrates based on their charge and size. It concludes with some applications of electrophoresis techniques.
Modern pharmaceutical analytical technique ( Electrophoresis)
This document provides an overview of electrophoresis, including definitions, principles, types, and applications. It discusses four main types of electrophoresis: paper electrophoresis, gel electrophoresis, capillary electrophoresis, and iso-electric electrophoresis. Paper and gel electrophoresis are described as zone electrophoresis techniques that separate components into bands on a supporting medium like paper or gel. Capillary electrophoresis allows separation in free solution within a thin capillary tube. Iso-electric electrophoresis separates molecules based on their iso-electric point, the pH where they have no net charge. The document outlines the principles, instrumentation, and applications of each electrophoresis technique.
Paper electrophoresis
Paper electrophoresis is a simple and inexpensive technique that uses filter paper and micro quantities of plasma to separate molecules like proteins, amino acids, and oligopeptides based on their charge. The basic equipment needed includes a power pack to provide a stabilized current and an electrophoretic cell containing electrodes, buffer reservoirs, and a support for the filter paper. Samples are applied to filter paper strips, which are placed in buffer solutions and an electric field is applied, causing charged particles to migrate and separate into distinct bands based on their size and charge. Paper electrophoresis is commonly used to analyze proteins in human plasma and other biological samples.
Electrophoresis
This document discusses electrophoresis, which is the migration of charged particles through a liquid medium under the influence of an electric field. It defines key terms and describes the theory behind electrophoresis, factors that influence particle migration rates, and different electrophoresis techniques. Some main techniques covered are agarose gel electrophoresis, polyacrylamide gel electrophoresis, isoelectric focusing, and two-dimensional electrophoresis. Troubleshooting tips for common issues are also provided.
Electrophoresispresentation
This document provides information about electrophoresis. It begins by defining electrophoresis as the migration of charged particles or molecules under the influence of an electric field. Some purposes of electrophoresis are to determine components in a sample, separate them, and obtain information about electrical double layers or determine molecular weights. The principle described is that charged particles migrate in an electric field at a velocity depending on factors like charge, size, shape and applied current. Different types of electrophoresis are described like paper, gel, and isolectric focusing electrophoresis. Key aspects of each technique including apparatus, sample application, detection, and preparation of gels are explained. Two-dimensional electrophoresis combining isolectric focusing and SDS-PAGE is also summarized.
electrophoresis
This document provides an overview of electrophoresis techniques. It begins with an introduction to electrophoresis as the migration of charged particles under an electric field. It then discusses various factors that affect electrophoresis like net charge, size, strength of electric field, buffer properties, and temperature. It describes different types of electrophoresis like paper, gel, cellulose acetate, isoelectric focusing, and moving boundary electrophoresis. Specific details are given about gel electrophoresis techniques like agarose, polyacrylamide gel, and SDS-PAGE electrophoresis. Other techniques like isolectric focusing, two-dimensional electrophoresis, isotachophoresis, pulsed field gel electrophoresis, and immunoelectrophoresis are also summarized briefly in 3 sentences
Electrophoresis
Electrophoresis is a method used to separate charged particles such as proteins and nucleic acids based on differences in their migration speed in an electric field. There are several types of electrophoresis including agarose gel electrophoresis, SDS-PAGE, isoelectric focusing, capillary electrophoresis, and two-dimensional electrophoresis. During electrophoresis, charged particles migrate through a gel or capillary towards an electrode with the rate of migration depending on factors like the particle's charge, size, and shape.
Electrophoresis
Electrophoresis is an analytical technique that separates and analyzes ionized analytes using their migration in an electric field. Charged molecules are separated as they migrate through a porous medium like agarose or polyacrylamide gel under the influence of an electric field generated between electrodes. The direction of migration depends on the charge, with positively charged ions or DNA fragments migrating toward the negative cathode and negatively charged molecules toward the positive anode. Different electrophoresis methods like agarose gel electrophoresis and polyacrylamide gel electrophoresis are used depending on the size of DNA fragments to be separated.
Basics of electrophoresis
Electrophoresis is a technique used to separate molecules based on their charge and size. It works by applying an electric field to move charged molecules through a medium such as a gel or paper. There are different types depending on the medium used, such as polyacrylamide gel electrophoresis and agarose gel electrophoresis. Electrophoresis has many applications including determining molecular weights, studying protein-protein interactions, and purifying proteins and DNA fragments.
gel electrophoresis by suresh b i
Gel electrophoresis is a method used to separate molecules like proteins and nucleic acids based on their physical properties. It works by applying an electric field to move charged molecules through a gel medium. Key aspects include applying samples to the gel, running electrophoresis with an electric current, and visualizing separated molecules by staining and quantification using a densitometer. Gel electrophoresis has applications in forensics, genetics, and biochemistry to analyze DNA, diagnose diseases, and study proteins. It separates biomolecules based on factors like size, shape, and charge.
Electrophoresis by Hina Ialsm Chishty
Electrophoresis is a technique used to separate charged molecules such as proteins and nucleic acids based on their charge and size ratios. It involves applying an electric field to migrate molecules through a medium like gel or paper. Initially developed in the 1930s to separate serum proteins, it has since been used for applications like human genome sequencing and disease diagnosis. Improvements to the technique include capillary electrophoresis using narrow tubes, isoelectric focusing to separate molecules at their isoelectric point, and immuno electrophoresis to study antibody-antigen reactions. A variety of detectors can identify separated molecules based on properties like UV absorption or electric resistance. Electrophoresis has applications in areas like forensics, clinical testing, and proteomics.
Electro phoresis sud mpharm pdf
The document provides an overview of electrophoresis, including:
1) Electrophoresis is a separation technique where charged molecules migrate at different rates in an electric field, allowing separation.
2) It is used to separate biological substances like proteins, nucleic acids, and amino acids.
3) There are different electrophoresis methods including capillary electrophoresis, which uses narrow capillaries, and zone electrophoresis using papers, gels, or thin layers to support molecules.
4) Factors like each molecule's charge, size, and the electric field strength determine their movement during electrophoresis.
Prabhakar singh ii sem-paper v-electrophoresis
Prabhakar singh ii sem-paper v-electrophoresisDepartment of Biochemistry, Veer Bahadur Singh Purvanchal Univarsity, Jaunpur
The document discusses electrophoresis, which is the movement of charged particles in an electric field. It migrates towards the oppositely charged electrode. Electrophoresis is used to separate biological molecules like proteins. It explains the principles and types of electrophoresis like paper, gel, and isolectric focusing electrophoresis. It provides details about agarose gel electrophoresis including properties, buffers used, procedure, and applications. It also briefly discusses polyacrylamide gel electrophoresis.Electrophoresis
Electrophoresis is an electrokinetic process which separates charged particles in a fluid using a field of electrical charge. It is most often used in life sciences to separate protein molecules or DNA and can be achieved through several different procedures depending on the type and size of the molecules. The procedures differ in some ways but all need a source for the electrical charge, a support medium and a buffer solution. Electrophoresis is used in laboratories for the separation of molecules based on size, density and purity.An electric field is applied to molecules and as they are electrically charged themselves it results in a force acting upon them. The greater the charge of the molecule the greater the force applied by the electrical field and therefore the further through the support medium the molecule will move relative to its mass.
Some example applications of electrophoresis include DNA and RNA analysis as well as protein electrophoresis which is a medical procedure used to analyse and separate the molecules found in a fluid sample (most commonly blood and urine samples).Different types of gels are usually used as the support medium for electrophoresis and this may be in slab or tube form depending on which is more beneficial. Gel slabs enable many samples to be run simultaneously and so are frequently used in laboratories. However, tube gels give a better resolution of the results so are often chosen for protein electrophoresis.
Agarose gel is commonly used for electrophoresis of DNA. It has a large pore structure allowing larger molecules to move easily but it is not suitable for sequencing smaller molecules.
Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose gel making it more suitable for quantitative analysis. This makes it possible to identify how proteins bind to DNA. It can also be used to develop an understanding of how bacteria is becoming resistant to antibiotics through plasmid analysis.
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Modern pharmaceutical analytical technique ( Electrophoresis)
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Similar to Electrophoresis
Electrophoresis
INTRODUCTION, DEFINATION OF ELECTROPHORESIS, ELECTROPHORESIS PRINCIPLE, TYPES OF ELECTROPHORESIS, FREE ELECTROPHORESIS, ZONE ELECTROPHORESIS,PAPER ELECTROPHORESIS, WORKING OF PAPER ELECTROPHORESIS, PROCEDURE FOR PAPER ELECTROPHORESIS, VISUALISATION, FACTORS AFFECTING SEPARATION OF MOLECULES, APPLICATIONS, working of paper electrophoresis ,procedure for paper electrophoresis ,visualisation ,factors affecting separation of molecules ,applications ,forensics ,dna fingerprinting ,molecular biology ,microbiology information about the organisms ,biochemistry mapping of cellular components ,paper electrophoresis is also used in study of sic ,hemoglobin abnormalities ,separation of blood clotting factors ,serum plasma proteins from blood sample ,used in separation and identification of alkaloids ,used for testing water samples ,toxicity of water ,drug industry to determine presence of illelgal drUGS
ELECTROPHORESIS AND ITS FORENSIC APPLICATIONS.pptx
Electrophoresis is a technique used to separate charged particles such as proteins, nucleic acids, and other molecules using an electric field. It was first observed in 1807 and involves applying an electric field to cause charged particles in solution to migrate toward the electrode of opposite charge. There are several types of electrophoresis including paper, gel, and capillary electrophoresis. Electrophoresis is widely used in forensic science applications such as DNA fingerprinting to identify suspects by comparing DNA profiles.
electrophorosis (1).pptx
This presentation discusses various types of electrophoresis, including paper electrophoresis, gel electrophoresis, capillary electrophoresis, and moving boundary electrophoresis. It explains the basic principles of electrophoresis, which involves the separation of charged particles in a conducting medium under the influence of an electric field. Factors that affect electrophoresis like charge, size, and pH are also summarized. The instrumentation and applications of each type of electrophoresis are briefly described.
Electrophoresis
Thank you for the detailed presentation on electrophoresis. I appreciate you taking the time to explain the key concepts and techniques. Please let me know if you have any other questions.
1 electrophoresis 1.pptx
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids. It works by applying an electric field to migrate molecules through a buffer solution or gel based on their size and charge. Key factors that affect migration rate are the net charge and size of the molecule, as well as the strength of the electric field and properties of the supporting medium used. Common electrophoresis methods include zone electrophoresis which uses a stabilizing gel matrix, and capillary electrophoresis where molecules are separated inside a thin capillary. The buffer system is also important as it carries current and determines the pH and charge of molecules during separation.
gelelectrophoresisfin-170426110518.pdf
Gel electrophoresis is a method to separate biomolecules like DNA, RNA, and proteins based on their size and charge. During gel electrophoresis, charged molecules are placed in wells in a gel and an electric current is applied, causing them to migrate through the gel at different rates depending on their size and charge. Larger molecules migrate more slowly through the gel pores than smaller molecules. This allows separation of molecules by size. Common gels used include agarose and polyacrylamide. Samples can be visualized after electrophoresis using dyes like ethidium bromide or stains. Gel electrophoresis has applications in DNA sequencing, forensic analysis, and medical research.
Electrophoresis
Electrophoresis is the migration of charged particles through an electric field towards the electrode of opposite charge. It works by separating molecules based on their size and charge. Key factors that affect electrophoresis include the net charge and size/shape of molecules, as well as the ionic strength, pH, temperature, and type of support medium used. Common applications include separating proteins, nucleic acids, and other biological molecules to analyze samples.
Gel electrophoresis
Gel electrophoresis is a method to separate biomolecules like proteins, nucleic acids, and lipids based on their charge and size. During gel electrophoresis, an electric current is applied across a gel, causing negatively charged molecules to migrate toward the positive electrode and positively charged molecules to migrate toward the negative electrode. Smaller molecules migrate faster through the gel than larger molecules. Factors like the charge, size, and shape of molecules, as well as the electric current, gel composition, and buffer used, determine how far each type of molecule will migrate through the gel. Gel electrophoresis has applications in separating DNA, RNA, proteins, and other biomolecules.
ELECTROPHORESIS.pptxHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuu...
ELECTROPHORESIS.pptxHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuu...anshikabhatnagar1299
Hhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuuqoqkwhbzklaiiwhhwkosishhwnaakoaizhbn1laHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuuqoqkwhbzklaiiwhhwkosishhwnaakoaizhbn1laHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuuqoqkwhbzklaiiwhhwkosishhwnaakoaizhbn1laHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuuqoqkwhbzklaiiwhhwkosishhwnaakoaizhbn1laHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuuqoqkwhbzklaiiwhhwkosishhwnaakoaizhbn1laElectrophoresis
Electrophoresis is the movement of charged particles through an electrode when subjected to an electric Field
Cations move towards cathode
Anions move towards anode
By this technique solutes are separated by their different rates of travel through an electric field.
Commonly used in biological analysis, particularly in the separations of proteins, peptides and nucleic acids
Electrophoresis
Electrophoresis is a general term that describes the migration and separation of charged particles under the influence of an electric field.
Electrophoresis
This document provides an overview of electrophoresis. It defines electrophoresis as the migration of electrically charged particles or ions in solutions due to an applied electric field. It describes the basic theory behind electrophoresis, including how electrophoretic mobility determines the speed at which ions or solutes move. It also outlines the key components of an electrophoresis apparatus and describes the basic procedure for performing electrophoresis, including sample preparation, gel preparation, running the electrophoresis, and visualizing the results. Finally, it discusses several applications of electrophoresis across various fields like forensics, molecular biology, genetics, microbiology, and biochemistry.
Applied Biochemistry
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids based on their size and charge. It involves applying an electric field to move molecules through a medium like agarose gel or capillary. Shorter/less charged molecules move faster and are separated from longer/more charged molecules. It is used in laboratories and clinical settings to analyze biological samples and diagnose conditions.
ELECTROPHORESIS 1.pptx
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids based on their charge and size. It works by applying an electric field to move molecules through a medium like a gel or paper. Smaller, more highly charged molecules move faster. There are several types of electrophoresis that differ in the medium used and application, such as paper electrophoresis using a filter paper medium and slab gel electrophoresis using a plastic backed gel to simultaneously separate multiple samples. Capillary electrophoresis uses a glass tube to yield fast, high resolution separations. Electrophoresis is useful for analytical applications in clinical chemistry and molecular biology.
GEL ELECTRPHORESIS
This document discusses gel electrophoresis, which separates biomolecules like DNA, RNA, and proteins based on their size and charge. It describes the basic principles of electrophoresis, including how mobility depends on factors like net charge and size. The key steps of gel electrophoresis are explained, such as preparing the gel, loading samples, running the current, and staining. Two-dimensional gel electrophoresis is also summarized, which separates proteins based on isoelectric focusing in the first dimension and size in the second dimension, allowing high resolution separation of thousands of proteins.
paper ELECTROPHORIS.pptx
Paper electrophoresis is a technique used to separate charged biomolecules like proteins and nucleic acids. It works by applying an electric field to a strip of filter paper soaked in buffer solution. The charged molecules migrate along the paper at different rates depending on their size and charge, separating into distinct bands. Key factors that affect separation are the properties of the sample molecules, the electric field strength and voltage gradient, the buffer composition and pH, and interactions with the paper medium. Paper electrophoresis has applications in clinical testing, forensic analysis, and environmental monitoring.
electrophoresis-170820132815-converted.pptx
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids based on their size and charge. It works by applying an electric current to move these molecules through a medium like a gel or paper. There are several types of electrophoresis that differ in the medium used, including gel electrophoresis, the most common, which uses an agarose or polyacrylamide gel to separate molecules by size. SDS-PAGE is a type of gel electrophoresis often used to separate proteins by molecular weight. Electrophoresis is widely applied in biomedical research and clinical diagnostics.
[Group 5] electrochemistry, electrophoresis, isoelectric focusing
This document discusses various principles of electrochemistry including concentration measurement using the Nernst equation, reference electrodes such as silver-silver chloride and calomel electrodes, indicator electrodes that are selective for specific ions, and ion-selective electrodes for measuring ions like hydrogen, sodium, and ammonium. It also covers principles of electrophoresis such as particle migration in an electric field, gel electrophoresis using agarose or polyacrylamide gels, and two-dimensional electrophoresis. Factors that affect electrochemical measurements and applications in clinical analysis are summarized.
ELECTROPHORESIS.pptx
This document provides an overview of electrophoresis, including its principle, working conditions, factors affecting separation, and types. Electrophoresis is an analytical technique that separates charged molecules like proteins and nucleic acids based on their movement in an electric field. It works by applying a voltage to move molecules through a buffer solution or gel support medium. The rate of migration depends on factors like the molecule's charge, size, and the electric field strength. Common electrophoresis techniques described include zone electrophoresis using paper, gels, and thin layers, as well as moving boundary methods like capillary electrophoresis.
Paper electrophoresis
Electrophoresis is a method used to separate charged molecules such as proteins and nucleic acids. It works by applying an electric field to encourage the migration of molecules towards the positively or negatively charged electrode, depending on their own charge. The document discusses the principle of electrophoresis, different types such as paper and zone electrophoresis, and factors that affect separation like charge, size, electric field strength, and buffer composition. It also outlines some applications in clinical testing, forensics, and environmental analysis.
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ELECTROPHORESIS AND ITS FORENSIC APPLICATIONS.pptx
ELECTROPHORESIS AND ITS FORENSIC APPLICATIONS.pptx
ELECTROPHORESIS.pptxHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuu...
ELECTROPHORESIS.pptxHhsjsjknsbajkakkajbsbbsnnznznsjshshbsnsjssjsjjjzjsjjkakuu...
[Group 5] electrochemistry, electrophoresis, isoelectric focusing
[Group 5] electrochemistry, electrophoresis, isoelectric focusing
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Electrophoresis
- 1. ELECTROPHORESIS GROUP NO 2 1. Attiqa Irshad 2. JAVERIA ASIF 3. SIDRA KHAN SHAHID 4. JOWARIA WAJIH 5. FARINA FARUQUI 6. NEELAM 7. FARHEEN JAVED 8. HADIQA 9. ANAM
- 2. ELECTROPHORESIS Electrophoresis :The term electrophoresis comes from the Greek means ,”transport by electricity”. In 1807,a Russian Physicist, Alexander Rauss observed a novel phenomenon- when electricity was passed through a glass tube containing water and clay ,colloidal particles moved towards the positive electrode when applied electric field. DEFINITION: Electrophoresis describes the migration of charged particles under the influence of electric field. PURPOSE FOR CARRYING OUT ELECTROPHORESIS: 1.To determine the number ,amount and mobility of compounds in given sample or to separate them. 2.To obtain information about the electrical double layers surrounding the particles. 3.Determination of molecular weight of proteins and DNA sequencing.
- 3. PRINCIPLE Any charged ion or molecule migrates when placed in an electric field .The rate of migration depend upon its net charge ,size , shape and the applied electric current. EQUATION: It can be expressed as; v=E q/f v=velocity of migration of the molecule E=electric field in volts per cm q=net electric charge on the molecule. f=frictional coefficient. The movement of charged particle in an electric field is expressed in terms of electrophoretic mobility denoted by u where, u=v/E OR u=q/f Electrophoretic mobility (u)of a molecule is directly proportional to charge density (charge/mass ratio).
- 5. Factors affecting Electrophoresis Mobility Charge : The higher the charge , greater is the electrophoresis mobility. The charge is dependent on pH of the medium. Size : The bigger molecules have a small electrophoretic mobility compared to the smaller particles. Shape : The globular protein will migrate fatser than the fibrous protein.
- 6. INSTRUMENTATION:- 1. Power Supply 2. 2 Buffer Boxes 3. Electrodes 4. Separation Medium
- 7. Power Supply Provides electrical power. Allows the adjustment and control of voltage and current. Helps in setting an electrical field between the 2 electrodes.
- 8. Buffer :- Gave multiple functions:- Carries the applied current. Maintains the pH. Determine the electrical charge on solute. Example: Tris Acetate- EDTA(TAE) for nucleic acid and DNA. Tri-borate- EDTA(TBE) for DNA.
- 9. Supporting medium is an matrix in which the protein separation take place various type has been used for the separation either on slab or capillary form separation is based on the charge to mass ratio of protein depending on the pore size of the medium , possibly the molecular size SUPPORTING MEDIUM
- 10. Chemical nature Inert Availability Easy PROPERTIES
- 11. Electric Conductivity High Absorptivity Low Sieving Effect Desirable
- 14. GENERAL OPERATION OR METHOD SAPERATION DETECTION QUANTIFICATION
- 15. GEL ELECTROPHORESIS KEY POINTS: Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.
- 17. HOW DO DNA FRAGMENTS MOVE THROUGH THE GEL Once the gel is in the box, each of the DNA samples we want to examine , is carefully transferred into one of the wells. One well is reserved for a DNA ladder, a standard reference that contains DNA fragments of known lengths.
- 18. VISUALIZING THE DNA FRAGMENTS
- 19. MODEL
- 20. CLINICAL APPLICATIONS OF ELECTROPHORESIS: 1. Lipoprotein Analysis. 2. Serum Protein Electrophoresis. 3. Cerebrospinal Fluid Analysis 4. Urine Analysis. 5. Small Molecules (like drugs and Steroid) Monitoring. 6. Diagnosis of Haemoglobinopathies and Hemoglobin A1c. 7. Genotype of Proteins e.g.. ApoE analysis for Alzheimer’s Disease (polymorphic proteins). 8. Determination of Serum Protein Phenotypes and Micro heterogeneities eg. Alpha-antitrypsin deficiency, MM.
- 21. Technical Considerations During operation of electrophoresis four point must be considered: Buffers Sample Stain Solution ELECTROENDOSMOSIS
- 22. Technical Consideration Buffer Should be in minor amount. After every cycle should change buffer coz ph Altered due to electrolysis of liquids. Should be used after cooler. Sample Appropriate amount of sample is used Avoid overloading Diluted serum uses
- 23. Technical Considerations STAIN SOLUTION Specific dye is used Inhibit vaporization ELECTROENDOSMOSIS Ionic cloud is mobile Positive ion moves towards cathode. Surface gel is negatively charged and should be immobile. Macromolecules are strongly charged and can alter the flow in opposite direction.
- 24. Technical Considerations In summarize form: