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Fungi –Yeasts and Molds
Dr C R Meera
Assistant Professor
Department of Microbiology
St Mary’s College, Thrissur-20
Kerala
FUNGI
• Eukaryotic spore bearing protists that
lack chlorophyll
• Consists of Yeasts and Molds
• Yeasts: Unicellular Fungi
• Molds: Filamentous Fungi
• Reproduction: Sexually and Asexually
• Exist as saprophytes, parasites or in
beneficial association with plants
(Mycorrhizae), algae (Lichens)
• Mycology: Study of fungi
IMPORTANCE OF FUNGI
• Heterotrophic saprophytes: Recycling of organic
compounds and increase soil fertility
• Mycorrhizae: Association with plant roots
• Industrial applications
Eg: Production of beer, wine, cheese, dough, antibiotics etc.
• Research Tool
• Decomposers of timber, textiles, food and other
materials
• Parasites : Human, animal and plant diseases
DISTINGUISHING CHARACTERISTICS OF FUNGI
• Eukaryotic chemoorganotrophic heterotrophs
• Thallus: Body of fungus
• Yeast : Single celled thallus
• Molds : Branched filamentous thallus
• Surrounded by true cell wall
• Some fungi show “Dimorphism”
• Lab diagnosis : Demonstration of dimorphism
Image courtesy: Wikipedia
YEASTS
• Larger than bacteria – 1-5 µm in
width & 5-30 µm in length
• Shape – Egg shaped, elongated or
spherical
• No flagella or locomotory organelles
• Each species has characteristic shape,
but depends on age and environment
• Reproduce by budding
• Identified by buds or bud scars
• Most widely used yeast:
Saccharomyces cereviceae
MOLDS
• Filamentous fungi
• Thallus consists of two parts: Mycelium and
spores
• Hyphae/Mycelium : septate or non-septate
• Spores: Resistant, resting dormant cells
• Spores on germination puts out “germ tube”
• Germ tubes elongate and branch to form
“Hyphae”
• Network of branched hyphae is called
“Mycelium”
Fungi on culture plates
Image courtesy: Wikipedia
Fungi under microscope
Image courtesy: bio.libretext.org
Fungi-Life cycle
Germ tube
Spore
Mycelium
Mushroom-Macrofungus
• Hyphae is about 5-10 µm in width
• Have true cell wall
Yeast Cell Morphology
• Single cells
• Cell wall
• Periplasm
• Plasma membrane
• Cytosole
• Nucleus
• Peroxisome, Golgi, ER,
Ribosome, Mitochondria,
Vacuoles, Secretory vesicles,
cytoskeleton etc.
MORPHOLOGY OF FUNGI
NUCLEUS
GOLGI BODIES
MITOCHONDRIA
PLASMA
MEMBRANE
SECRETORY
VESICLES
CELL
WALL
ER
VACUOLE
MORPHOLOGY OF FUNGI
Morphology of Molds
• Multi cellular
• Hyphae with outer tube like cell wall
• Cavity of hyphae is called lumen which is filled with protoplasm
• Plasmalemma- Double layered membrane between cell wall & protoplasm
• Cell wall = Matrix + Micro fibrils
• Matrix – Proteins, lipids and other substances
• Micro fibrils – Hemicellulose, Chitin & true cellulose (lower fungi)
• Nucleus and other cellular components
STRUCTURE OF FUNGAL HYPHAE
GROWTH OF FUNGAL HYPHAE
• Growth at distal end
• Division by cross wall formation by centripetal invagination
• Cross walls grow inwards and constrict plasmalemma
• Incomplete septum with central pores
• Protoplasmic streaming is common
• Cell division followed by Karyogamy
CELL
WALL
SEPTUM
CYTOPLASM
SEPTAL
PORE
NUCLEI
FORMS OF FUNGAL HYPHAE
Hyphae occur in three forms
• Coenocytic - Non septate
• Monokaryotic - Septate uninucleated cells
• Septate Dikaryotic or Multinucleated cells
Septate Multinucleate hypha
TYPES OF FUNGAL MYCELIA
• Two types: Vegetative and
Reproductive hyphae
• Vegetative or submerged
mycelium : To absorb nutrients,
penetrate into medium
• Reproductive mycelium : Spore
production, protrude out from
media
AERIAL
REPRODUCTIVE
HYPHAEAR
AERIAL
VEGETATIVE
HYPHAEAR
SURFACE
HYPHAEAR
PENETRATIVE
HYPHAEAR
THANK YOU

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Fungi- Yeast and Molds- Dr C R Meera

  • 1. Fungi –Yeasts and Molds Dr C R Meera Assistant Professor Department of Microbiology St Mary’s College, Thrissur-20 Kerala
  • 2. FUNGI • Eukaryotic spore bearing protists that lack chlorophyll • Consists of Yeasts and Molds • Yeasts: Unicellular Fungi • Molds: Filamentous Fungi • Reproduction: Sexually and Asexually • Exist as saprophytes, parasites or in beneficial association with plants (Mycorrhizae), algae (Lichens) • Mycology: Study of fungi
  • 3. IMPORTANCE OF FUNGI • Heterotrophic saprophytes: Recycling of organic compounds and increase soil fertility • Mycorrhizae: Association with plant roots • Industrial applications Eg: Production of beer, wine, cheese, dough, antibiotics etc. • Research Tool • Decomposers of timber, textiles, food and other materials • Parasites : Human, animal and plant diseases
  • 4. DISTINGUISHING CHARACTERISTICS OF FUNGI • Eukaryotic chemoorganotrophic heterotrophs • Thallus: Body of fungus • Yeast : Single celled thallus • Molds : Branched filamentous thallus • Surrounded by true cell wall • Some fungi show “Dimorphism” • Lab diagnosis : Demonstration of dimorphism
  • 5. Image courtesy: Wikipedia YEASTS • Larger than bacteria – 1-5 µm in width & 5-30 µm in length • Shape – Egg shaped, elongated or spherical • No flagella or locomotory organelles • Each species has characteristic shape, but depends on age and environment • Reproduce by budding • Identified by buds or bud scars • Most widely used yeast: Saccharomyces cereviceae
  • 6. MOLDS • Filamentous fungi • Thallus consists of two parts: Mycelium and spores • Hyphae/Mycelium : septate or non-septate • Spores: Resistant, resting dormant cells • Spores on germination puts out “germ tube” • Germ tubes elongate and branch to form “Hyphae” • Network of branched hyphae is called “Mycelium” Fungi on culture plates Image courtesy: Wikipedia Fungi under microscope Image courtesy: bio.libretext.org
  • 8. • Hyphae is about 5-10 µm in width
  • 9. • Have true cell wall Yeast Cell Morphology • Single cells • Cell wall • Periplasm • Plasma membrane • Cytosole • Nucleus • Peroxisome, Golgi, ER, Ribosome, Mitochondria, Vacuoles, Secretory vesicles, cytoskeleton etc. MORPHOLOGY OF FUNGI NUCLEUS GOLGI BODIES MITOCHONDRIA PLASMA MEMBRANE SECRETORY VESICLES CELL WALL ER VACUOLE
  • 10. MORPHOLOGY OF FUNGI Morphology of Molds • Multi cellular • Hyphae with outer tube like cell wall • Cavity of hyphae is called lumen which is filled with protoplasm • Plasmalemma- Double layered membrane between cell wall & protoplasm • Cell wall = Matrix + Micro fibrils • Matrix – Proteins, lipids and other substances • Micro fibrils – Hemicellulose, Chitin & true cellulose (lower fungi) • Nucleus and other cellular components
  • 12. GROWTH OF FUNGAL HYPHAE • Growth at distal end • Division by cross wall formation by centripetal invagination • Cross walls grow inwards and constrict plasmalemma • Incomplete septum with central pores • Protoplasmic streaming is common • Cell division followed by Karyogamy CELL WALL SEPTUM CYTOPLASM SEPTAL PORE NUCLEI
  • 13. FORMS OF FUNGAL HYPHAE Hyphae occur in three forms • Coenocytic - Non septate • Monokaryotic - Septate uninucleated cells • Septate Dikaryotic or Multinucleated cells Septate Multinucleate hypha
  • 14. TYPES OF FUNGAL MYCELIA • Two types: Vegetative and Reproductive hyphae • Vegetative or submerged mycelium : To absorb nutrients, penetrate into medium • Reproductive mycelium : Spore production, protrude out from media AERIAL REPRODUCTIVE HYPHAEAR AERIAL VEGETATIVE HYPHAEAR SURFACE HYPHAEAR PENETRATIVE HYPHAEAR