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Mycoplasma
This document summarizes a seminar presentation on Mycoplasma. Key points include: - Mycoplasma are the smallest bacteria capable of autonomous growth. They lack cell walls and are sensitive to osmotic shock and antibiotics like tetracyclines. - They were first discovered in 1843 and isolated from cattle pleural fluid. Techniques for culturing mycoplasma were developed in the late 19th century. - Mycoplasma can take different shapes and sizes. They reproduce through binary fission or budding. - They are classified as facultative anaerobes in the class Mollicutes. Some require sterols for growth while others do not.
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Mycoplasma
- 1. “Mycoplasma” Seminar presented by AshishDiwakar M.Sc.Microbiology II Semester School of Studies in Microbiology Vagdevi Bhavan, Vikram University,Ujjain (M.P.)
- 2. Synopsis • Introduction ofMycoplasma • History of Mycoplasma • Morphology of Mycoplasma • Chemical composition of mycoplasma • Classification of Mycoplasma • Reproduction of Mcoplasma • Structure of Mycoplasma • Pathogenecity of mycoplasma • Treatment • Conclusion • References
- 3. Introduction • Unicellular Prokaryote •Gram-Positive Bacteria • Lack cell wall- Pleomorphic • Osmotic Shock / Detergent-Sensitive • Antibiotics-Tetracyclin / Chloremphenicol-Sensitive • They are the smallest bacteria capable of autonomous growth • Facultative anaerobes / obligate anaerobes • Low G+C%, 23-41% • Mostly require sterol for their growth • (but some genera do not require sterol – such as Acholeplasma, Asteroleplasma, Mesoplasma)
- 4. History • Discovered byPasture in 1843. • Isolated from pleural fluid of cattle. • It was called as Mycoplasma mycoides. • Nocard & Roux first cultured Mycoplasma in1898. • In 1929, Nowak called them PPLO.
- 5. Morphology Size :- 300nmto 0.2nm Shape :- Coccoidal,Diploform,Filamentus,Spiral shape &Teardrop shape.
- 6. Colonical appearance &Colony Cheracterstics:- • Colony developed on the solied Medium. • Show nippled or fried egg colony.
- 7. Chemical Composition • Protein- 40-60% • Carbohydrate - 0.1% • DNA Content - 3-7% • Lipid - 8-20%
- 8. CLASSIFICATION Kingdom : Bacteria Division: Firmicutes Class : Mollicutes Order : Mycoplasmatales Family : Mycoplasmataceae Genus : Mycoplasma
- 9. Reproduction of Mycoplasma Binaryfission Budding or Round body formation Filamentous formation
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- 12. Pathogenesity • Extra cellularparasite • It Causes infection in Respiratory tract & Urinogenital tract • In Respiratory tract Cause Pneumonia • In Urinogenital tract Cause Male sterility Diseases Causeing Mechenism of Mycoplasma Attachment :- Host cell - Have - Glycoprotein
- 13. Penetration:- • Mucosa membranepenetrate • Attach on epithelial cells • Growth on epithelial cells • And destroy epithelial cells • Cause Pneumonia Treatment:- • Tetracyclin / Chloramphenicol / Erythromycin
- 14. Conclusion • Mycoplasma arepeculier type of bacteria • as they requiring sterol for growth • They are cell wall less • some are pathogenic (humen diseses) • some are cattle/plants/insects parasite also
- 15. References Willey J.M.,L.M.Sherwood &C.J. Woolverton (2008) Prescott, Harly & Klein’s Microbiology,Seventh Edition,MC Graw Hill, Page no.571 PELCZAR M.J.,E.C.S. CHAN & N.R. KRIEG 2007 Microbiology Fifth Edition,MC Graw Hill,Page no.281,805 Madigan,M.T.,Martinko,J.M.and Parker, (2000) Brock Biology of Microorganisms,Sixth Edition Prentice Hall International Inc.