Fourier Transform Ion Cyclotron Resonance (FTICR)

1. Welcome

2. Seminar Presentation on
Fourier Transform Mass
Spectrometry and Fourier
Transform Ion Cyclotron
Resonance (FTICR) Analyser
Presented by

3. Fourier Transform Mass Spectrometry
and Fourier Transform Ion Cyclotron
Resonance (FTICR) Analyser
Contents
Fourier Transform Mass Spectrometry
 Introduction
 Advantages and Limitations
Fourier Transform Ion Cyclotron Resonance
(FTICR)
 Introduction
 Principle of Operation

4. Fourier Transform Mass Spectrometry
 Fourier Transform Mass Spectrometry (FTMS) is an
analytical technique that measure the mass to
charge ratio and abundance of ion in sample
 FTMS uses a strong magnetic field to trap ions in a
circular orbit and then excites them with a resonant
pulse to create a coherent orbit. The frequency of
the orbit is inversely proportional to the ions mass
to charge ratio.
 FTMS has several advantages
◦ High sensitivity and resolution
◦ Fast and accurate
◦ Dilute Sample

5. Fourier Transform Mass Spectrometry
 FTMS also has some limitations
◦ Expensive
◦ Large Size
◦ Complex Operation
◦ Challenging data interpretation

6. Fourier Transform Ion Cyclotron
Resonance (FTICR) Analyser
 Fourier transform ion cyclotron resonance
mass spectrometry is a type of mass
spectrometer for determining the mass to
charge ratio of ion based on the cyclotron
frequency of the ion in a fixed magnetic field.
 FTICR is the highest performance mass
spectrometry technique available offering
unrivalled resolution and mass accuracy

7. Fourier Transform Ion Cyclotron
Resonance (FTICR) Analyser

8. Fourier Transform Ion Cyclotron
Resonance (FTICR) Analyser
 Based on cyclotron frequency an ion is fixed on a
magnetic field
 Ion are trapped in a penning trap where they are
excited based on cyclotron frequency (excited by
radio wave)
 While exciting they are converted to larger
cyclotron radius by an oscillating electric field
orthogonal to magnetic field.
 After the removal of excitation field, ions are
rotating at their cyclotron frequency (as a packet of
ions)

9. Fourier Transform Ion Cyclotron Resonance
(FTICR) Analyser
 These ions induce a charge which are detected
by imaging.
 Lower molecular weight atom which moves
faster and generate separate image graph
 Medium molecular weight atom which moves
less faster and generate different image graph
 High molecular weight atom which moves
slower than others generate separate image
graph

10. Thank You….!