Proteomics presentation on fticr

1. ESSORE UNIVERSITY OF SCIENCE & TECHNOLOGY
A
PRESENTATION ON
Fourier Transform Ion Cyclotron Resonance
Presented by
MD ROBEL AHMED
ROLL NO: 14 06 03
4TH YEAR 2ND SEMESTER
DEPT. OF GENETIC
ENGINEERING AND
BIOTECHNYLOGY
Presented to
DR. MD. MASHIAR RAHMAN
ASSISTANT PROFESSOR
DEPT. OF GENETIC
ENGINEERING AND
BIOTECHNYLOGY
DATE OF SUBMISSION: 15 SEPTEMBER 2018

3. INDEX
 History of invention
 Fourier Transform
 Cyclotron mechanism
 Principle
 Basic structure of the analyzer
 FTICR Mechanism
 Advantages & Disadvantages
 Applications
 References

4. HISTORY OF INVENTION
Invented by Melvin B
Comisarow and Alan G
Marshall at the
UNIVERSITY OF
BRITISH COLUMBIA.
First publication done in
1974 on the journal on
chemical physics letter.

5. CYCLOTRON MECHANISM Magnetic field
Electric field

6. Fourier Transform
t
f(Ѡ)
Ѡ
f(t)

7.  Types of mass analyzer determining mass to
charge ratio of ion
 It is based on cyclotron frequency of the ion in a
fixed magnetic field
 Ions are trapped in a penning trap where they are
excited(based on cyclotron frequency.
 While exciting they converted to larger cyclotron
radius by an oscillating electric field orthogonal to
magnetic field.
 After the removal the excitation field, ions are
rotating at their cyclotron frequency (as a packet
PRINCIPLE

8. BASIC STRUCTURE OF THE ANALYZER

9. Radio frequency
source
Data
output
Detector plate
resistor
amplifier
ions
Excitation plate
RF
FTICR
MECHANISM

10. ANALYSIS OF THE RESULT
Ѡ=
𝑞𝐵
𝑚
1
f =
𝑞𝐵
2¶𝑚
2
ESI FT-ICR mass spectrum of 20-|M ubiquitin in an
aqueous 10-mM ammonium acetate solution, measured in
terms of relative intensity versus m/z.

11. ADVANTAGES OF FTICR
DISADVANTAGES OF FTICR
 High Accuracy
 Electrospray ionization
 Mass accuracy
 Extreme high resolution
 Costly(expensive at $1-2million for a
standard instrument)
 Operation require highly skilled
personnel

12. FIELD OF IMPLEMENTATION
• High resolution is also useful in studying large
macromolecules such as proteins with multiple
charges,
• Isotope analysis
• Useful in analyzing phosphorylation states, O-
or n-linked glycosylation, and sulfating

13. REFERENCES
https://nationalmaglab.org
https://en.wikipedia.org/wiki/Fourier-
transform_ion_cyclotron_resonance
https://www.youtube.com/watch?v=7EHngA4S3Ws
www.massspecpro.com
www.bumc.bu.edu/ftms/modern-instrumental-
biochemistry/ftms-introduction/
http://what-when-how.com/proteomics/ft-icr-
proteomics/