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Samplecollection& Preservation
10/6/2022
Toxicology & Forensic Serology 1
Dr. Aamir Ali Khan
Assistant Professor
PhD Biochemistry
1
• SampleRequired for Forensic &toxicological Testing
– Blood
– Urine
– Stomachlavage/stomach aspirate
– Saliva,
– Stainedcloth (blood/semen)
– Pubic/Vaginalswab (semen/DNA)
– Hair /HairFollicles
5/7/2021
Toxicology & Forensic Serology 2
• SampleCollectionAfterPost-Mortem
– HumanViscera
• Stomach
• Small intestine
• Liver
• Kidney
• Urine/blood/vitereous
• Heart
4
•If Patient isAlive & suspicionofpoisoning
Material Quantity Remarks
Vomit/stomach
Aspirate
300 ml if less available Whole
quantity
Stomach washout 500 ml if less available Whole
quantity
Blood 10 ml Preferably more , 100 ml
Urine 100-200
ml
if less available Whole
quantity
*Sampleshould beproperly preservedand labeled
5
Material Quantity Remarks
Stomach Whole N/A
Small intestine 100 cm adult 200 cm children, whole in infants,
preferably tied end to end
Liver 100 ml/whole Preferably the portion with gall bladder
Spleen 500 mg 5cm thick slice of liver or whole
in infants
Kidneys Half in adult Whole in children
Vitreous Maximum As much as can be withdrawn
•After autopsy specimen to bepreserve irrespective of thenatureof
poison
5/7/202
1
Toxicology & Forensic
Serolog
y
•Additional sample required in special Circumstances
Nature of Poison Material
(to be preserved)
Quantity
Alcohol Peripheral
blood Vitreous
10 ml
Vitreous maximum
Drug of abuse (barbiturates, opium
etc)
Blood 10 ml
Carbon Monoxide Blood 10 ml
Heavy metal (chronic
arsenic poisoning etc)
Bon
e
Hair
Nais
Shaft of bone 10
cm Scalp hair with
roots Toe and
finger nails
Inhalation/volatile substance Lung Air tigh container, tie off trachea
Injected poison Skin, subcutaneous tissue/muscle from
injection mark, similar tissue
opposite side as control
7
Special Circumstances Material
(to be preserved)
Criminal Abortion Vagina, uterus, ovaries, any thing related to genital tract
Decomposed Bodies Insect egg, maggots and pupa
Exhumed bodies Soil samples from above, beneath & sides of coffin or any fluid
in coffin . Control sample from distance away of coffin
Embalmed bodies Embalming fluids, bone marrow
Stained cloths Stained cloth and surrounding unstained area as control
•Special Circumstances
8
Sample Condition Location Collection procedure
Liquid (blood) Scene/hospital Collect in EDTA, through syringe or
gauze/cotton and air dry it
Clot (blood) Scene Transfer clot to glass tube add equal volume of
saline, transfer to cotton cloth & dry
Wet (blood) Cloth Air dry the cloth at room temperature and
transfer to paper bag , avoid contamination
Wet (blood) Object Air dry at room temperature and transfer to
gauze / paper envelope
Stain (blood) unmovable
surface e.g
floor
Collect by swab DO NOT SCRAP
(double swab method)
9
Sample Condition Preservative
Urine Saturated soln/rectified spirit/hydrochloric acid
Human Viscera's
(liver, stomach, kidney, etc)
Saturated saline if there is no chance of acid poison
Rectified spirit if poisoned by acid
Heart (histopathological
examination)
10 percent formalin
Maggots Dropped alive into boiling absolute alcohol or 10 %
hot formalin
Blood in carbon monoxide
poisoning
A layer of 1-2 cm liquid paraffin should be added
immediately over the collected blood to avoid
exposure to atmospheric oxygen
1
0
• Human visceraarepreserved infour differentwide
mouthed glass bottle
• Bottle should befilled2/3rdandsome room for gases
– Bottle I: Stomachandintestinewithitscontent
– Bottle II: kidney,liverandspleen
– Bottle III: mayhavespecialsample,heart,brain etc
– Vial : separatevialfor eachBlood and urine
– Bottle IV: Only preservativeactascontrol
1
1
• Lidofbottledshould betightlysealedandlabeled
• Bottlewillbeplacedinviscerabox (wooden box) sealedand
labeled again
• Keyofbox sealedinenvelop,handoverto policewith proper receiving
• Policeconstablecarry itto FSLlab alongwith
– Copy ofpanchanama,
– Autopsy report,requisitionletterbyMO to FSL Lab
1
2
• Medical jurisprudence, forensicmedicineandtoxicology,BV Subrahmanyam
SexualAssault Evidence Collection
1
3
Dr. Aamir Ali Khan
Assistant Professor
PhD Biochemistry
1
4
• Sexualserious offensearoundthe globe
• Forensicprofessionalsfocusedon theidentification, recovery,packing,andanalysisof
evidence.
• Evidencecollectionto D N A profiling,needtimely approach&propertools to
utilize
• The spermcellsmore resistantthensomaticto biologicaldegradation
– Thanks to Spermnucleus(protamine),protectfrom nucleases
16
18
19
20
21
• Biological EvidenceAnalysis inCases ofSexual AssaultDOI:http://dx.doi.org/10.5772/intechopen.82164
5/7/2021

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Forensic Sample Collection and Preservation.pptx

  • 1. Samplecollection& Preservation 10/6/2022 Toxicology & Forensic Serology 1 Dr. Aamir Ali Khan Assistant Professor PhD Biochemistry
  • 2. 1 • SampleRequired for Forensic &toxicological Testing – Blood – Urine – Stomachlavage/stomach aspirate – Saliva, – Stainedcloth (blood/semen) – Pubic/Vaginalswab (semen/DNA) – Hair /HairFollicles
  • 3. 5/7/2021 Toxicology & Forensic Serology 2 • SampleCollectionAfterPost-Mortem – HumanViscera • Stomach • Small intestine • Liver • Kidney • Urine/blood/vitereous • Heart
  • 4. 4 •If Patient isAlive & suspicionofpoisoning Material Quantity Remarks Vomit/stomach Aspirate 300 ml if less available Whole quantity Stomach washout 500 ml if less available Whole quantity Blood 10 ml Preferably more , 100 ml Urine 100-200 ml if less available Whole quantity *Sampleshould beproperly preservedand labeled
  • 5. 5 Material Quantity Remarks Stomach Whole N/A Small intestine 100 cm adult 200 cm children, whole in infants, preferably tied end to end Liver 100 ml/whole Preferably the portion with gall bladder Spleen 500 mg 5cm thick slice of liver or whole in infants Kidneys Half in adult Whole in children Vitreous Maximum As much as can be withdrawn •After autopsy specimen to bepreserve irrespective of thenatureof poison
  • 6. 5/7/202 1 Toxicology & Forensic Serolog y •Additional sample required in special Circumstances Nature of Poison Material (to be preserved) Quantity Alcohol Peripheral blood Vitreous 10 ml Vitreous maximum Drug of abuse (barbiturates, opium etc) Blood 10 ml Carbon Monoxide Blood 10 ml Heavy metal (chronic arsenic poisoning etc) Bon e Hair Nais Shaft of bone 10 cm Scalp hair with roots Toe and finger nails Inhalation/volatile substance Lung Air tigh container, tie off trachea Injected poison Skin, subcutaneous tissue/muscle from injection mark, similar tissue opposite side as control
  • 7. 7 Special Circumstances Material (to be preserved) Criminal Abortion Vagina, uterus, ovaries, any thing related to genital tract Decomposed Bodies Insect egg, maggots and pupa Exhumed bodies Soil samples from above, beneath & sides of coffin or any fluid in coffin . Control sample from distance away of coffin Embalmed bodies Embalming fluids, bone marrow Stained cloths Stained cloth and surrounding unstained area as control •Special Circumstances
  • 8. 8 Sample Condition Location Collection procedure Liquid (blood) Scene/hospital Collect in EDTA, through syringe or gauze/cotton and air dry it Clot (blood) Scene Transfer clot to glass tube add equal volume of saline, transfer to cotton cloth & dry Wet (blood) Cloth Air dry the cloth at room temperature and transfer to paper bag , avoid contamination Wet (blood) Object Air dry at room temperature and transfer to gauze / paper envelope Stain (blood) unmovable surface e.g floor Collect by swab DO NOT SCRAP (double swab method)
  • 9. 9 Sample Condition Preservative Urine Saturated soln/rectified spirit/hydrochloric acid Human Viscera's (liver, stomach, kidney, etc) Saturated saline if there is no chance of acid poison Rectified spirit if poisoned by acid Heart (histopathological examination) 10 percent formalin Maggots Dropped alive into boiling absolute alcohol or 10 % hot formalin Blood in carbon monoxide poisoning A layer of 1-2 cm liquid paraffin should be added immediately over the collected blood to avoid exposure to atmospheric oxygen
  • 10. 1 0 • Human visceraarepreserved infour differentwide mouthed glass bottle • Bottle should befilled2/3rdandsome room for gases – Bottle I: Stomachandintestinewithitscontent – Bottle II: kidney,liverandspleen – Bottle III: mayhavespecialsample,heart,brain etc – Vial : separatevialfor eachBlood and urine – Bottle IV: Only preservativeactascontrol
  • 11. 1 1 • Lidofbottledshould betightlysealedandlabeled • Bottlewillbeplacedinviscerabox (wooden box) sealedand labeled again • Keyofbox sealedinenvelop,handoverto policewith proper receiving • Policeconstablecarry itto FSLlab alongwith – Copy ofpanchanama, – Autopsy report,requisitionletterbyMO to FSL Lab
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  • 13. 1 2 • Medical jurisprudence, forensicmedicineandtoxicology,BV Subrahmanyam
  • 14. SexualAssault Evidence Collection 1 3 Dr. Aamir Ali Khan Assistant Professor PhD Biochemistry
  • 15. 1 4 • Sexualserious offensearoundthe globe • Forensicprofessionalsfocusedon theidentification, recovery,packing,andanalysisof evidence. • Evidencecollectionto D N A profiling,needtimely approach&propertools to utilize • The spermcellsmore resistantthensomaticto biologicaldegradation – Thanks to Spermnucleus(protamine),protectfrom nucleases
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  • 22. • Biological EvidenceAnalysis inCases ofSexual AssaultDOI:http://dx.doi.org/10.5772/intechopen.82164 5/7/2021