1) Nitrogen use efficiency (NUE) refers to the efficiency with which a plant acquires nitrogen from the soil and utilizes it for growth and grain production. Improving NUE is important to reduce environmental pollution from nitrogen loss and decrease production costs.
2) NUE is a complex trait influenced by both internal genetic factors and external environmental conditions. It has two main components - nitrogen uptake efficiency and nitrogen utilization efficiency. Key physiological and molecular mechanisms controlling NUE include nitrogen transport, assimilation, remobilization and storage.
3) Various approaches can be used to improve NUE in crops, including marker-assisted breeding to introgress quantitative trait loci associated with NUE, transgenic methods to modify expression of genes regulating
Indian agriculture feels the pain of fatigue of green revolution.
In the past 50 years, the fertilizer consumption exponentially increased from 0.5 (1960’s) to 24 million tonnes (2013) that commensurate with four-fold increase in food grain output (254 million tonnes) In order to achieve a target of 300 million tonnes of food grains and to feed the burgeoning population of 1.4 billion in 2025, the country will require 45 million tonnes of nutrients as against a current consumption level of 23 million tonnes. The sustainable agriculture and precision farming both are the urgent issues and hence the suitable agro-technological interventions are essential (e.g., nano and biotechnology) for ensuring the safety and sustainability of relevant production system.
Indian agriculture feels the pain of fatigue of green revolution.
In the past 50 years, the fertilizer consumption exponentially increased from 0.5 (1960’s) to 24 million tonnes (2013) that commensurate with four-fold increase in food grain output (254 million tonnes) In order to achieve a target of 300 million tonnes of food grains and to feed the burgeoning population of 1.4 billion in 2025, the country will require 45 million tonnes of nutrients as against a current consumption level of 23 million tonnes. The sustainable agriculture and precision farming both are the urgent issues and hence the suitable agro-technological interventions are essential (e.g., nano and biotechnology) for ensuring the safety and sustainability of relevant production system.
PATHWAY OF MOVEMENT OF ASSIMILATES IN DEVELOPING GRAINS OF MONOCOTS AND DICOT...kartoori sai santhosh
PATHWAY OF MOVEMENT OF ASSIMILATES IN DEVELOPING GRAINS OF MONOCOTS AND DICOTS
CHEMICAL COMPOSITION OF SEEDS
STORAGE OF CARBOHYDRATES, PROTEINS AND FATS IN SEEDS AND THEIR BIOSYNTHESIS
Definition and introduction of fertilizer use efficiency , Causes for Low and Declining Crop Response to Fertilizers and FUE.Methods to increase fertilizer use efficiency.
Use of nanofertilizers on fruit trees contributes effectively to improve the fruit quality and increasing the productivity of trees. It reduces environmental pollution by reducing the amount of fertilizers used, which is positively reflected in the increased economic return of the farmers. When nanofertilizers sprayed at very low concentration on fruit trees, these compounds have had a direct effect by increasing the growth, yield and quality of these fruit crops.
CS_701_Nitrate Assimilation by arnold_damasoAr R Ventura
Nitrate assimilation is the formation of organic nitrogen compounds like amino acids from inorganic nitrogen compounds present in the environment. Organisms like plants, fungi and certain bacteria that cannot fix nitrogen gas (N2) depend on the ability to assimilate nitrate or ammonia for their needs.
Plants like castor reduce a lot of nitrate in the root itself, and excrete the resulting base. Some of the base produced in the shoots is transported to the roots as salts of organic acids while a small amount of the carboxylates are just stored in the shoot itself. However, about 99% of the organic nitrogen in the biosphere is derived from the assimilation of nitrate. NH4+ is formed as an end product of the degradation of organic matter, primarily by the metabolism of animals and bacteria, and is oxidized to nitrate again by nitrifying bacteria in the soil. Thus a continuous cycle exists between the nitrate in the soil and the organic nitrogen in the plants growing on it. While nearly all the ammonia in the root is usually incorporated into amino acids at the root itself, plants may transport significant amounts of ammonium ions in the xylem to be fixed in the shoots. This may help avoid the transport of organic compounds down to the roots just to carry the nitrogen back as amino acids.
PATHWAY OF MOVEMENT OF ASSIMILATES IN DEVELOPING GRAINS OF MONOCOTS AND DICOT...kartoori sai santhosh
PATHWAY OF MOVEMENT OF ASSIMILATES IN DEVELOPING GRAINS OF MONOCOTS AND DICOTS
CHEMICAL COMPOSITION OF SEEDS
STORAGE OF CARBOHYDRATES, PROTEINS AND FATS IN SEEDS AND THEIR BIOSYNTHESIS
Definition and introduction of fertilizer use efficiency , Causes for Low and Declining Crop Response to Fertilizers and FUE.Methods to increase fertilizer use efficiency.
Use of nanofertilizers on fruit trees contributes effectively to improve the fruit quality and increasing the productivity of trees. It reduces environmental pollution by reducing the amount of fertilizers used, which is positively reflected in the increased economic return of the farmers. When nanofertilizers sprayed at very low concentration on fruit trees, these compounds have had a direct effect by increasing the growth, yield and quality of these fruit crops.
CS_701_Nitrate Assimilation by arnold_damasoAr R Ventura
Nitrate assimilation is the formation of organic nitrogen compounds like amino acids from inorganic nitrogen compounds present in the environment. Organisms like plants, fungi and certain bacteria that cannot fix nitrogen gas (N2) depend on the ability to assimilate nitrate or ammonia for their needs.
Plants like castor reduce a lot of nitrate in the root itself, and excrete the resulting base. Some of the base produced in the shoots is transported to the roots as salts of organic acids while a small amount of the carboxylates are just stored in the shoot itself. However, about 99% of the organic nitrogen in the biosphere is derived from the assimilation of nitrate. NH4+ is formed as an end product of the degradation of organic matter, primarily by the metabolism of animals and bacteria, and is oxidized to nitrate again by nitrifying bacteria in the soil. Thus a continuous cycle exists between the nitrate in the soil and the organic nitrogen in the plants growing on it. While nearly all the ammonia in the root is usually incorporated into amino acids at the root itself, plants may transport significant amounts of ammonium ions in the xylem to be fixed in the shoots. This may help avoid the transport of organic compounds down to the roots just to carry the nitrogen back as amino acids.
Directrice adjointe de l'Institut Jean-Pierre Bourgin (Institut de recherche végétale, INRA de Versailles) Françoise Vedèle fait un focus sur la génétique et les effets de l'azote dans la nutrition des plantes.
Rice Root physiology work at CIAT: Identification of ideal root system to imp...CIAT
Water and Nitrogen are quantitatively the most essential resources for plant growth. Active root systems that can take up water and nutrients more efficiently are essential for enhancing grain yield. However, it is difficult to find the ideal root system to improve water and Nitrogen uptake because the root growth was sensitive and affected by environment such as drought and nutrient deficiency conditions. However, there were several reported that some constitutive root traits and root controlling genes (QTLs) to improve water and nitrogen uptake (Uga et al. 2013; Arai-Sanoh et al. ; Ogawa et al. 2016) .
Here, we examined with root modified breeding lines using both marker assisted selection and transgenic technology under stress conditions. Using 48 chromosome segment substitution lines (CSSLs) between Curinga; commercial upland rice in Brazil and Oryza rufipogon (IRGC105491); a non-sativa species, we found total 15 QTLs including a QTL for nitrogen-deficiency tolerance in grain yield on chromosomes 1. In addition to QTLs identification, we observed that the dimorphic roots system (that has both shallow and deep roots system) from Oryza rufipogon trait correlated to Nitrogen deficiency tolerance in grain yield under field conditions. Using DEEPER ROOTING 1 (DRO1) inserting transgenic lines, we found higher expression of DRO1 increases the root growth angle, whereby roots grow in a more downward direction. Introducing DRO1 into IR64; a shallow-rooting rice cultivar enabled the resulting line to maintained high yield performance under both water and Nitrogen deficient conditions relative to the recipient cultivar. The result showed us the deeper rooting traits is useful to absorb water and Nitrogen from the deeper layer under both water and nitrogen deficiency conditions.
In the future, these identified root system and genes (QTLs) will shed light on root architecture systems in rice breeding to improve agronomic performance under stress conditions.
Can changes in root anatomical traits during stress enhance drought & Salini...kabeya
There are array of ways of studying plant response to drought or any kind of stress, ranging from physiological, morphological, cellular level, biochemical, anatomical or even at molecular level. This presentation deals or shows how plant tissues can respond under stress at anatomical level and hence contribution to tolerance.
Chapters 18 - Amino acid Oxidation , production of urea Biochemistry Areej Abu Hanieh
Chapters 18 - Amino acid Oxidation , production of urea Biochemistry
the link for chapter 22 : https://www.slideshare.net/arijabuhaniyeh/chapters-18-22-biochemistry-74720233
Nitrogen Use Efficiency (NUE ) is defined as the yield obtained per unit of available nitrogen (N) in the soil
Efficiency with which the plant uses N from acquired available N to total plant dry matter
NUE is the product of uptake efficiency and utilization efficiency
It is required in all environmental conditions where ever yield of plant is required , NUE -> crop yield
To minimize N loss, maximize N uptake and reduce environmental pollution
NUE is a complex quantitative traits which involves many genes
Expression of multiple gene depend on a number of internal and external factors
There are 100s of nitrate responsive genes
For their transcription require regulatory sequence i.e., NRE (Nitrate responsive elements)
One such element originally reported to be comprised of an A[G/C]TCA sequence
These sequence are randomly distributed throughout the genome
QTL mapping is a powerful tool for analysis of complex NUE
Candidate genes encoding enzyme that involved in N uptake, assimilation and utilization have been reported in rice, maize, arabidopsis etc
Genetic variation and phenotypic plasticity for NUE
Determine the level of genetic variation - landraces and genotypes
Study a defined genetic population under different N conditions
Interactions between N uptake and water availability
Interaction between different macronutrients and micronutrients
Genotype by environment (G × E) interaction
Modifying the root system
Modern approaches of nitrogen management in rice.pptxPankajLochanPanda
Among plant nutrients, Nitrogen plays a crucial role in growth and yield of the crops. Owing to its importance it is subjected to indiscriminate application which inturn gives rise to several ill effects such as environmental and water pollution. Therefore it is of paramount importance to manage Nitrogen in an efficient way.
IMPROVING NITROGEN USE EFFICIENCY IN PLANTS hj.pptx34JeevanrajR
Nitrogen, the vital primary plant growth nutrient at deficit soil conditions, drastically affects the growth and yield of a crop. Over the years, excess use of inorganic nitrogenous fertilizers resulted in pollution, eutrophication and thereby demanding the reduction in the use of chemical fertilizers. Being a C4 plant with fibrous root system and high NUE, maize can be deployed to be the best candidate for better N uptake and utilization in nitrogen deficient soils. The maize germplasm sources has enormous genetic variation for better nitrogen uptake contributing traits. Adoption of single cross maize hybrids as well as inherent property of high NUE has helped maize cultivars to achieve the highest growth rate among the cereals during last decade. Further, considering the high cost of nitrogenous fertilizers, adverse effects on soil health and environmental impact, maize improvement demands better utilization of existing genetic variation for NUE via introgression of novel allelic combinations in existing cultivars.
Furthermore, there is reason to question the usefulness of many traditional NUE formulations, and to consider factors to improve the conceptualization of NUE for future use. As a resource for agricultural researchers and students, here we present a comprehensive list of NUE indices and discuss their functions, strengths, and limitations. We also suggest several factors—which are currently ignored in traditional NUE indices—that will improve the conceptualization of NUE, such as: accounting for a wider range of soil N forms, considering how plants mediate their response to the soil N status, including the below-ground/root N pools, capturing the synchrony between available N and plant N demand, blending agronomic performance with ecosystem functioning, and affirming the biological meaning of NUE.
In the last six decades, the consumption of reactive nitrogen (Nr) in the form of fertilizer in India has been growing rapidly, whilst the nitrogen use efficiency (NUE) of cropping systems has been decreasing. These trends have led to increasing environmental losses of Nr, threatening the quality of air, soils, and fresh waters, and thereby endangering climate-stability, ecosystems, and human-health. Since it has been suggested that the fertilizer consumption of India may double by 2050, there is an urgent need for scientific research to support better nitrogen management in Indian agriculture. In order to share knowledge and to develop a joint vision, experts from the UK and India came together for a conference and workshop on “Challenges and Opportunities for Agricultural Nitrogen Science in India.” The meeting concluded with three core messages: Soil stewardship is essential and legumes need to be planted in rotation with cereals to increase nitrogen fixation in areas of limited Nr availability.
Performance of Sesame (Sesamum indicum L.) Under Different Supplementary Irri...CrimsonpublishersMCDA
Field experiment was conducted in Humera in 2015 main cropping season in a factorial randomized complete block design (RCBD) to study the performance of sesame under different supplementary irrigation applications and nitrogen fertilizer rates. The study consists of four levels of nitrogen fertilizer rates (0kg N2/ha, 23kgN2/ha, 37.5kgN2/ha and 46kgN2/ha) and three levels of supplementary irrigation; rain-fed (I0), four irrigations (I4), and six irrigations (I6). Nitrogen fertilizer was applied in two splits, with the first half at sowing and the remaining half 30 days after emergence. Data on plant height, number of capsules per plant, number of seeds per capsule, thousand seed weight, harvest index, aboveground biomass and grain yield were recorded. The analysis of variance (ANOVA), in indicated that application of nitrogen fertilizer significantly (p<0.05) affected sesame grain yield. The highest grain yield 423.4kg/ ha, and 455kg/ha was obtained from application of 46kg nitrogen per hectare, and six supplementary irrigation. The results obtained in this experiment showed that, application of supplementary irrigation could be an important climate change adaptation strategy in areas where onset and cessation of rainfall occur.
Credit of peanut to subsequent wheat under desert farming conditions in prese...IJAEMSJORNAL
Rotation with leguminous crops to break non-legume monocultures has been established to benefit the latter. The lacking information on this cultivation system in stressed environments encouraged the implementation of two field trials in two different locations of Ismailia desert soils. The experimental design included the cultivation of wheat subsequent to peanut in presence of diazotroph inoculation and N fertilization. Bradyrhizobial inoculation of the legume in combination with 50 kg N acre-1 resulted in the highest total biological yields of 4.24 and 5.01 kg plot-1 at the experimental sites 1 and 2, respective seed yields of 1.46 and 1.61 kg pot-1 were recorded. In case of the cereal crop, the measured acetylene reducing activities in soils of associative diazotroph-inoculated plants together with 50 kg N acre-1 were the highest being 515.8-886.2 and 616.7-1066.2 nmoles C2H4 g-1 h-1 at locations 1 and 2 respectively. The enzymatic activity of fallow-cultivated wheat generally represented ca. 95 % of that in subsequent to peanut. Irrespective of inoculation and N fertilization, the wheat biomass yield increases in residual effect-field over the fallow one were 6.4-35.1 % and 4.6-38.5 % at experimental sites 1 and 2 respectively. Increase percentages of 3.1-26.6 and 6.9-44.7 were scored as well for grain yield. The beneficial residual effect of the legume to the succeeding cereal was also extended to protein yields, increases of 4.0-14.2 % and 4.5-7.6 % were estimated for grain protein as well as 8.3-24.1 % and 8.1-35.3 % for straw protein yield. The findings of this study proved that the positively yield turnover of a legume is extended to the subsequent non-legume. Besides, the beneficial residual effects of legumes toward rotated non legumes could be magnified by diazotroph inoculation together with adequate N supply particularly in stressed environments represented, in the present study, by Ismailia sandy soil.
A Comprehensive research study on effect on combine application of nitrogen and boron on yield and Growth of sunflower.
By Ravi Banjade
IAAS TU, Nepal
Email : rabibanjade3@gmail.com
Effects of Nitrogen Fertilization and interZiziphus Jujubealleys cropping on ...Agriculture Journal IJOEAR
Abstract— In order to test the effects of intercropping and nitrogen fertilization on blue panic (panicum antidotale) yield, a field experiment was conducted at the Agricultural Research Station of King Abdulaziz University, located at Hada Al-Sham during two consecutive seasons (2013 and 2014). Blue panic was intercropped between interspaces alleys of Ziziphus Jujube under three different levels of nitrogen fertilizer (0 kg N/ha, 200 kg N/ha and 400 kg N/ha in the form of commercial Urea,46%) and three distances from the jujube tree (1m ,2m and 4m). Blue panic fresh and dry forage yield (tons/ha) and quality was assessed during ten cuttings harvests. The results exhibited significant effects of intercropping, nitrogen fertilization and distance from the tree on forage yield and quality. Forage yield across all ten harvests were higher under intercropped plots compared to sole crops and in plots treated with 200 and 400 kg N and 2-meter distance from the trees. Total fresh forage yield /ha/10 cuts of the intercropped blue panic under 400 kg N/ha and 2m distance reached 186 tons/ha/year. Whereas the highest protein content means overall the different cuts was 11.51% in plots fertilized with 400 kg N/ha at 1m distance from the trees. This current study highlights the importance of utilizing jujube alleysto maximize land use value of this widely planted arid land tree.
Keywords— Forage yield, Forage quality, Jujube tree, alley cropping.
This is a seminar paper about nano-fertilizer for agricultural application prepared by Md. Parvez Kabir, an MS Student under the department of Soil Science of Bangabandhu Sheikh Mujibur Rahman Agricultural University. This paper helps to know how it increases the nutrient use efficiency, yield and decreases the toxicity effect and cost of crop cultivation.
Biological Nitrogen Fixation
Contents:
Introduction
Methods for measuring N2 fixation
1. Ntrogen balance method
2. Nitrogen difference method
3. Ureides method
4.〖𝟏𝟓〗_𝑵 isotope techniques
5. Acetylene reduction assay
6. Hydrogen evolution method
Introduction
N2 gas are found 78.084%on atmosphere of earth.
Nitrogen is an essential element for plant growth and development and a key issue of agriculture.
N2 are found in molecular N2 (𝑵 ≡ 𝑵) form in soil.
Dinitrogen is more stable, so we need of nitrogen fixation.
Most studies indicate that nitrogen fertilizers contribute to resolving the challenge the world is facing, feeding the human population.
The Green revolution was accompanied by an enormous increase in the application of nitrogen fertilizer.
Nitrogen fixation is a process by which nitrogen of the Earth's atmosphere is converted into ammonia (NH3), nitrogen salts or other molecules available to living organisms.
Biological Nitrogen Fixation(BNF) is known to be a sustain agriculture and increase soil fertility.
Research on microorganisms and plants able to fix nitrogen contributes largely to the production of bio fertilizers.
Thus it is important to ensure that BNF research and development will take into account the needs of farmers in the developing countries mainly.
Role of nitrogen in Plant
Sources of Nitrogen
Why measure 𝑵_𝟐 fixation?
Ecological consideration require an understanding of the relative contribution of 𝑵_𝟐 fixing components to the N-cycle.
Measurement of 𝑁_2 fixation enable an investigator to evaluate the ability of indigenous Rhizobium spp. to effectively nodulate newly introduced legumes.
Development of sustainable farming systems.
Understanding of the amount of 𝑵_𝟐fixed by legumes as influenced by soil management or cultural practices allows development of efficient agricultural and agroforesty production systems.
Effect of Azotobactor inoculation with Nitrogen levels on Yield and Quality o...Santosh pathak
The important vegetable-cum-spice crops of Nepal botanically referred to as genus Capsicum.
The native to the Tropical South America and Brazil.
Two species such as C. annuum and C. frutescens are commonly cultivated throughout the world.
India-largest producer of chilli in the world (Khan and Raj, 2006)
Good source of vitamins A, C, E, B1and B2, Potassium, phosphorus and calcium
Similar to Physiological and Molecular basis of NUE (20)
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
DERIVATION OF MODIFIED BERNOULLI EQUATION WITH VISCOUS EFFECTS AND TERMINAL V...Wasswaderrick3
In this book, we use conservation of energy techniques on a fluid element to derive the Modified Bernoulli equation of flow with viscous or friction effects. We derive the general equation of flow/ velocity and then from this we derive the Pouiselle flow equation, the transition flow equation and the turbulent flow equation. In the situations where there are no viscous effects , the equation reduces to the Bernoulli equation. From experimental results, we are able to include other terms in the Bernoulli equation. We also look at cases where pressure gradients exist. We use the Modified Bernoulli equation to derive equations of flow rate for pipes of different cross sectional areas connected together. We also extend our techniques of energy conservation to a sphere falling in a viscous medium under the effect of gravity. We demonstrate Stokes equation of terminal velocity and turbulent flow equation. We look at a way of calculating the time taken for a body to fall in a viscous medium. We also look at the general equation of terminal velocity.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. Contents
Introduction.
Need for improvement of NUE
Understanding plant response to N limitation
Physiological basis of NUE
Molecular basis of NUE
Approaches for improving NUE
Case study
Ideotype of Nitrogen use efficient genotype
The relationship between NUE and grain yield
Conclusion
3.
4. What is NUE ?
(Yield obtained per unit of available nitrogen (N) in the soil)
Physiological NUE =Eefficiency with which the plant
uses N from acquired available N to total plant dry matter
N imported from the field to the crop product
Agronomic NUE =
N applied.
NUE is the product of uptake efficiency and utilization
efficiency.
NUE in plants is a complex phenomenon that depends on
a number of internal and external factors.
5. Where, When and Why NUE traits
are required?
It’s required in all environmental condition where yield is
required, because NUE crop yield.⍺
For abiotic stress improvement in crops, NUE has become the
second priority after drought both in the private and in the
public sector.
To minimize N loss, maximize N uptake & reduce
environmental pollution.
It’s required in all environmental condition where yield is
required, because NUE crop yield.⍺
For abiotic stress improvement in crops, NUE has become the
second priority after drought both in the private and in the
public sector.
To minimize N loss, maximize N uptake & reduce
environmental pollution.
7. Global Status of N fertilizer consumption
& NUE
Global Status of N fertilizer consumption
& NUE
There is 20-fold increase in the global use of N fertilizer
applications during the past five decades (Glass, 2003) and
this is expected to increase at least threefold by 2050 (Good et
al., 2004).
The current average NUE in the field is approximately 33%
and substantial proportion of the remaining 67% is lost into
the environment, especially in the intensively cropped area.
It is estimated that 1% increase in NUE could save $1.1 billion
annually.
8. Need for improvement of NUE
Nitrogen (N) must be supplemented to meet the food production
demands of an ever-increasing population.
The cost of mineral nitrogen fertilizer accounts for a major
portion of the total cost of production.
N recovery by crops is only 30% to 35% of that applied and
remaining 65% to 70% is lost into the environment through a
combination of ammonia volatilization, denitrification, leaching,
immobilization and runoff.
Therefore, Physiologist and plant breeders need to develop
cultivars that can exploit N more efficiently in order to minimize
loss of N, reduce environmental pollution, decrease input cost and
make more economic use of the absorbed N.
9. Understanding plant response to N
limitation
• There may be two type of response to N limitation (i.e.)
1. Adaptive response and
2. Non-adoptive response
Adaptive responses
Increase in N uptake.
Retardation of growth and photosynthesis.
Remobilization of N from older to young leaves and reproductive parts.
Increased anthocyanin accumulation.
Non -adaptive responses
Fail to accumulate anthocyanin.
Having sudden senescence.
Unable to remobilize N metabolites from rosette leaves towards
developing seeds.
10.
11. Physiological components of NUE
There may be a two components of NUE
1. Nitrogen Uptake Efficiency
2. Nitrogen Utilization Efficiency
12. 1. Nitrogen uptake efficiency
(Amount of absorbed N/quantity of available N)
N uptake efficiency drives biomass production and depend on
Amount of N uptake
Storage
Assimilation
13. N uptake (Kant & Rothstein., 2010)
For plant N is mainly available in the form of NO3
-
and NH4
+
There are 3 type of NO3
-
transporter - LATS, cHATS & iHATS
Km value of cHATS-(about 6-20 μM) [Forde and Clarkson, 1999]
Km value of iHATS-(about 13-79 μM) [Forde and Clarkson,
1999]
Several structural gene involve in N uptake
NRT1 families member (NRT1.1 and NRT1.2) are characterized as
LAT
NRT2 families member (NRT2.1 and NRT2.2) are characterized as
14. N Assimilation
NO3
-
after entering into the plant cell, assimilation is begin .
N Storage
Absorbed N may stored in the vacoule or directly assimilate
into amino acid
18. 2. Nitrogen Utilization Efficiency
(Efficiency of a plant to utilized N from acquired available N
in the plant for production of biomass)
Utilization efficiency involved N remobilization.
At the reproductive stage both N assimilation & N
remobalization become critical.
Leaves & shoot act as a source for amino acid to the
reproductive and storage organ.
Upto 80% of the grain N content is derived from leaves in
rice and wheat.
During leaf senescence N release via protease activities.
19. Amino acid
The predominant gene families which involved in phloem
loading process is AAP (AAP1 - AAP8)
Loading
into
Phloem
NH4
+
Stored Protein
21. Genetics of NUE
NUE is a complex quantitative traits which involves many
genes.
Expression of multiple gene depend on a number of
internal and external factors.
There are 100s of nitrate responsive gene.
For their transcription require regulatory sequence i.e.
NRE (Nitrate responsive element).
22. One of the such sequence originally reported to be
comprised of an A[G/C]TCA sequence.
These sequence is randomly distributed throughout the
genome.
QTL mapping is a powerful tool for analysis of complex
NUE.
By using QTL mapping in some model spp. such as rice,
arabidopsis and maize, candidate genes encoding enzyme
that involved in N uptake, assimilation and utilization have
been identified.
Contd. (genetics of NUE)
27. Agronomic Approach
Optimum N Application_________________
According to phonological stages,
Based on the crop N demand,
Knowledge of plant N uptake efficiency,
Based on soil N status or
Using controlled-release fertilizers - Nitroform®
- Nutralene®
- IB Nitrogen
28. NUE is affected by
N uptake efficiency - Root characteristics
Physiological N use efficiency (PE)
-mode of photosynthesis i.e. C3 or C4
- N supply
N partitioning b/w leaf & stem
Efficiency of N use in CO2 fixation
Increasing leaf N content
Delay leaf senescence
Higher canopy structure
Physiological Approach
32. Marker-assisted backcrossing (MAB)
There are three levels of selection in which markers may be
applied in backcross breeding.
Foreground selection : to screen forthe target trait.
Recombinant selection: to minimize linkage drag.
Background selection: to the recovery of the recurrent
parent genome.
33. Transgenic approach
(Abrol, Raghuram & Sachdev, 2007)
GENE GENE SOURCE TARGET PLANT
PHENOTYPE
OBSERVED
Nrt 2.1
Nicotiana
plumbaginifolia
N. tabaccum
Increased nitrate influx
under low N conditions
NR N. plumbaginifolia N. tabaccum
Higher
Nitrate assimilation
NiR Spinacea oleracea Arabidopsis
Higher
Ammonium
accumulation
GS1 Glycine max Lotus corniculatus Accelerated senescence
35. Identified a major QTL (TOND1) on the long arm of
chromosome 12, confer tolerance to nitrogen deficiency in Teqing
YIL 105 x Teqing
F1
311 F2
34
75
41
Contain TOND 1 Don’t Contain TOND1
150 cultivars (75 Indica and 75 Japonica cultivars) and regions
36. Whether rice cultivars having the TOND 1 allele
exhibit strong tolerance to N deficiency ?
Over-expression of TOND1 increased the tolerance to N
deficiency in the TOND1- deficient rice cultivars.
Identification of TOND 1 provides
a molecular basis of breeding
for breeding rice varieties with
improved grain yield despite input
of N fertilizer
Concluded
37. Ideotype of Nitrogen use efficient genotype
Increased amount and activity of NR & NiR
Increased amount and activity of GS & GOGAT
High root to shoot ratio
High root vigor & root hair
High root length density
N-induced root proliferation
Microbial symbiosis
38. Grain yield is positively correlated with N uptake
efficiency and N utilization efficiency
Grain yield is positively correlated with N uptake
efficiency and N utilization efficiency
39. There is a considerable genetic variability for NUE which
provide opportunity for genetic improvement.
Several candidate gene responsible for NUE have been identify
in several crops like Arabidopsis, Rice and maize etc.
By hybridization programme, its is enable to transfer
identified NUE related QTL to the genotype having good
agronomic background.
Incorporation of NUE trait in a genotype will increase
the ultimate grain yield which will contribute to
our national economy.