Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and Semi-Field Conditions
Coptoterme formosanus is an economically important agricultural and structural pest of warm and humid regions of the world. The main objective of the study was evaluate seed extracts of Brassica nigra and leaves extracts of Acokantra schimperi, Croton macrostachyus and Rhamnus prinoides against C. formosanus workers under laboratory and semi-field conditions. Treatments were consisted of three concentrations levels (5, 10 and 15 weight of botanical powder (g) per 100 ml volume of water) by three replications. Mortality of termite was counted after 24, 48 and 72 hours exposure for both conditions. The results of all botanical extracts at all concentration levels showed that caused mortality of C. formosanus workers. Complete mortality (100%) of C. formosanus was observed after treatment with 15 w/v B. nigra extract at three time intervals under both laboratory and semi-field conditions. Moreover, A. schimperi at 15 w/v concentration also resulted 100% mortality after 48-72 hours of exposure. Brassica nigra extract showed least LC50 (5.63g/100ml) value than other botanical extracts after 24 hours exposure under laboratory condition. Based on their toxicity status extracts of B. nigra > A. schmperi > R. prinoides > C. macrostachyus leaf extracts.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Multidrug resistant microorganisms are globally becoming a major confrontment because of illogical use of antibiotics and this played a good role in investigation about the antibacterial compounds in plants. Thus, the present study investigate for the antibacterial effect of alcoholic extracts of Curcuma longa L. rhizomes , CommiphoramyrrhaL. gums and Ginkgo biloba L. leaves products against Staphylococcus aureus and Pseudomonas aeruginosa. The plants samples extracted by soxhlet with methanol and fractionation with and four solution ( chloroform, hexane, water and ethyl acetate) were used for investigation about antibacterial activity by disc diffusion method. The results showed that methanolic alcohol extract and fractions of C. longa L. rhizomes , C. myrrha L. gums showed biological activity against P. aeruginosa and S. aureus bacteria, but methanolic alcohol extract and fractions of G. biloba L. leaves product didn’t show any activity as antibacterial substance. It can be concluded that the presence of secondary metabolites as flavonoids, alkaloids, tannins, glycosides and saponins in the plants under study would be marked a good anti-bacterial effect.
Indian medicinal plants have a traditional
background that they have potential to use as antimicrobial agents.
Pedalium murex showed broad spectrum antimicrobial activity
against three fungal strains Aspergillus niger, Aspergillus flavus,
Candida albicans and five bacterial strains Escherichia coli,
Staphylococus epidermis, Klebseilla pneumonia,Citrobactor
diverses, Enterococus faecalis.The Ethanolic extracts were tested
against selected test bacteria and fungi through disc diffusion assay
where amoxicillin was used as standard. The results showed that
alcoholic extract possess good antimicrobial activity against selected
test bacteria and fungi. The present results therefore offer a
scientific basis for traditional use of the various extract of Pedalium
murex
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
a) Methods used in sampling, extraction of motile stages and cysts,
b) Different groups of plant parasitic nematodes found in soil samples,
c) Calculate the ratio of eggs and cysts in soil samples as well as number of nematodes in a 100ml of soil,
d) Effects of plant parasitic nematodes on a susceptible and resistant variety of fodder radish.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Multidrug resistant microorganisms are globally becoming a major confrontment because of illogical use of antibiotics and this played a good role in investigation about the antibacterial compounds in plants. Thus, the present study investigate for the antibacterial effect of alcoholic extracts of Curcuma longa L. rhizomes , CommiphoramyrrhaL. gums and Ginkgo biloba L. leaves products against Staphylococcus aureus and Pseudomonas aeruginosa. The plants samples extracted by soxhlet with methanol and fractionation with and four solution ( chloroform, hexane, water and ethyl acetate) were used for investigation about antibacterial activity by disc diffusion method. The results showed that methanolic alcohol extract and fractions of C. longa L. rhizomes , C. myrrha L. gums showed biological activity against P. aeruginosa and S. aureus bacteria, but methanolic alcohol extract and fractions of G. biloba L. leaves product didn’t show any activity as antibacterial substance. It can be concluded that the presence of secondary metabolites as flavonoids, alkaloids, tannins, glycosides and saponins in the plants under study would be marked a good anti-bacterial effect.
Indian medicinal plants have a traditional
background that they have potential to use as antimicrobial agents.
Pedalium murex showed broad spectrum antimicrobial activity
against three fungal strains Aspergillus niger, Aspergillus flavus,
Candida albicans and five bacterial strains Escherichia coli,
Staphylococus epidermis, Klebseilla pneumonia,Citrobactor
diverses, Enterococus faecalis.The Ethanolic extracts were tested
against selected test bacteria and fungi through disc diffusion assay
where amoxicillin was used as standard. The results showed that
alcoholic extract possess good antimicrobial activity against selected
test bacteria and fungi. The present results therefore offer a
scientific basis for traditional use of the various extract of Pedalium
murex
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
a) Methods used in sampling, extraction of motile stages and cysts,
b) Different groups of plant parasitic nematodes found in soil samples,
c) Calculate the ratio of eggs and cysts in soil samples as well as number of nematodes in a 100ml of soil,
d) Effects of plant parasitic nematodes on a susceptible and resistant variety of fodder radish.
Invitro Evaluation of Antimicrobial Activity of the plant extracts of Elytrar...IJERA Editor
Antimicrobial activity of the aerial parts of the Elytraria acaulis a stem less perennial herb of Acantheceae family has been carried out in the present study. Extracts of the aerial parts of the plant (Stem & Leaves) were prepared in different organic solvents such as n-Hexane, Ethanol, Methanol and Ethyl acetate. All the extracts were analyzed for antimicrobial properties against various pathogenic bacterial infections (Escherichia coli, Klebsiella Species, and Salmonella Typhi). Amongst all the extracts, methanolic extract exhibited significant antimicrobial activity. The crude methanol extracts of leaves showed good inhibitory effects against pathogens. Hence further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications.
Evaluation of Antimicrobial Activity of Excoecaria Agallocha Lijtsrd
Excoecaria agallocha L. is an important medicinal plant inhabited in mangrove regions. Early researches focused on antimicrobial activity of leaves of concerned plant with various solvents among which ethanol, chloroform and methanol were Used. Ella. Sai Kumar "Evaluation of Antimicrobial Activity of Excoecaria Agallocha L" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-3 , April 2020, URL: https://www.ijtsrd.com/papers/ijtsrd30276.pdf Paper Url :https://www.ijtsrd.com/biological-science/microbiology/30276/evaluation-of-antimicrobial-activity-of-excoecaria-agallocha-l/ella-sai-kumar
Antimicrobial activity of herbal productionkarimbscdu
The use of plants in treatment of burns, dermatophytes and infectious diseases is common in traditional medicine. The development of new antimicrobial agents against resistant pathogens is increasing interest. Therefore, the methanolic extracts from different parts of four medicinal plants used locally in folk medicine were evaluated for antimicrobial activity. It was found that most plant extracts studied had antibacterial and antifungal activities. The methanolic extract of leaf of the plant Azadiracta indica, Acacia nilotica and Witania somnifera showed significant antibacterial activity against Bacillus subtilis, Escherchia coli, stphaylocuccus aureus and pseudomonas fluorescence. Azadiracta indica and A.tinolica showed significant antifungal activity against A. flavus, Ziziphus mauritiana. The rhizome extract of curcuma longa showed significant activity against all tested bacteria and showed higher anti fungal activity against Fusarium verticillioides
ABSTRACT- Phylloplane fungi presented on the leaf surface were screened and selected for the assessment of their
potential against Alternaria alternata causing leaf spot disease of Rauwolfia serpentina (Sarapgandha). Among the
antagonists Trichoderma harzianum ISO-2 showed minimum conidial germination. Effect of foliar spray of phylloplane
fungi on the lesion development was also studied. In mist chamber studies seven treatments comprising application of
antagonists in the presence of pathogen proved effective in causing per cent disease reduction by 30-50 % in comparison
with the control. In the field trials Penicillium sublateritium showed the maximum fresh and dry root weight. P. sublateritium
also showed significant percentage disease reduction in comparison to control.
Key words- Alternaria alternata, Foliar spray, Leaf spot, Phylloplane fungi, Rauwolfia serpentina
Biological Efficacy of Quassia Indica (Geratn) Nooteb and Centella Asiatica (...IOSRJPBS
Antimicrobial activity of different extracts of two selected plants, Quassia indica (Geartn) Nooteb and Centella asiatica (L.) Urban were tested against selected strains of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas sps, Bacillus thuringiensis, Klebsiella sps and Serratia sps using agar disc diffusion technique. Petroleum ether, acetone, methanol and water extracts of seed and leaf of Q. indica and leaf of C. asiatica showed moderate to significant antimicrobial activity. Of the four extracts tested, petroleum ether and water fractions showed more activity against all the bacterial strains. The zone of inhibition of the active fractions for the petroleum ether ranged from 8.57 to 12.63 mm and 8.53 to 14.5mm for the water fraction of Q. indica seed and leaf and C. asiatica leaf; and exhibited comparable results with widely used commercial antimicrobial agents (16 to19 mm for Kanamycin and 14 to 17mm for Cefotaxime). The results prove the efficacy of the most active fractions of the selected plant extracts to be used for developing potent antimicrobial formulations.
Effect of environmental pollution on the quality of an edible plant Alternant...Premier Publishers
The present study is the comparative analysis of phytochemical constituents and microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb collected from unpolluted and polluted site. Preliminary phytochemical analysis was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts (unpolluted and polluted site) of A philoxeroides that showed the presence of alkaloids, carbohydrates, saponins, phenols, flavonoids, aminoacids, diterpenes, tannin, terpenoids, protein, steroid, oxalate, coumarin and quinones. The ethanol extract showed higher number of phytochemical constituents when compared to the other extract of unpolluted site. The microbial load is also enumerated in the unpolluted and polluted site. In conclusion, phytochemical analysis revealed the presence of many phytoconstituents in ethanol extract and the microbial load is less in the unpolluted site when compared to the polluted site.
Evaluation of antibacterial activity of some medicinal plants used by Sugali ...researchplantsciences
In the present work an attempt has been made to carry out screening for the preliminary antibacterial activity of different plants used by Sugali tribes of Yerramalis forest. Fifteen plants were selected for preliminary screening for their antibacterial potentiality, The antibacterial activity was done against four bacterial strains, viz., Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli,.The preliminary screening experiment revealed that methanol extracts were more potent than the aqueous extracts. The most susceptible bacteria were K. pneumoniae and the most resistant bacteria were E. coli. Bauhinia racemosa L. exhibited remarkable antibacterial activity.
Article Citation:
Khaleel Basha S, Sudarsanam G, Hari Babu Rao D, Niaz Parveen.
Evaluation of antibacterial activity of some medicinal plants used by Sugali tribe of Yerramalais forest of Andhra Pradesh, India.
Journal of Research in Plant Sciences (2011) 1(1): 027-031.
Full Text:
http://plantsciences.co.in/documents/PS0003.pdf
Trichosporon sp. are widely distributed in nature and can predominantly be found in the environmental substrates, such as soil, birds, vegetables, water, and decomposing wood. These fungi can colonize skin and, less frequently, respiratory and gastrointestinal tracts of humans. Currently, the genus Trichosporon sp. is considered one of the most important emerging causes of invasive infection in immune compromised patients, with T. asahii being the most frequently reported species. In this study, T. asahii was procured from MTCC and its sensitivity was checked against different solvents (Methanol, Ethanol, Acetone, and Chloroform) of Iris ensata, a medicinal plant. Zone of inhibition and MIC were evaluated using Agar well diffusion assay and Tube dilution broth assay. Ketoconazole and Nystatin B were used as positive controls. Phytochemical screening was done to determine the phytochemicals present in the plant. The methanol extract was found to be most effective compared to other solvent extracts and positive controls. Phytochemicals play a major role in this anti Trichosporon activity. Key-words- Yeast infection, Trichosporon, Medicinal plant, Phytochemicals test
ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT EXTRACS AGAINST PLANT PATHOG...IJSIT Editor
The aim of this work was to find an alternative to chemical fungicides currently used in the control
plant pathogenic fungi Rhizoctoniasolani ,ColletotrichummusaeandFusariumoxysporum,. The antifungal
activity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.All
plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID)
against above fungi. The media amended with methanol and recommended fungicide for respective fungal
strain were consider as negative and positive control respectively.Results showed that radial growth in all the
three tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culture
medium used. The test fungi differed in their reaction to the different extracts but on the whole, growth
inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of
the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R.
solani and C.muceawhereas those from wild basil inhibited the growth of the same pathogens by 89%, 84%
and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild
basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet
flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed
60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and
wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the
potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthe...Premier Publishers
The present study analyses the antimicrobial activity and the microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb growing in polluted and unpolluted site. The plants were collected and tested against various Gram positive, Gram negative bacteria and fungi. Antimicrobial activity was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts of aerial parts A. philoxeroides collected from polluted and unpolluted site that showed significant antimicrobial activity against tested bacterial and fungal organisms. The extracts were compared with standards like Amoxicillin for antibacterial activity and Ketoconazole for antifungal activity. The extracts showed remarkable antimicrobial activity as measured from the zone of inhibition and results were comparable with that of standard drugs against the organisms tested. The microbial load is also enumerated in the cooked and cooked refrigerated samples from polluted and unpolluted site. In conclusion, plant extract of A. philoxeroides collected from polluted site showed less antimicrobial activity and higher antimicrobial activity in unpolluted site. The ethanol extract showed higher activity when compared to other extracts. The microbial load is higher in cooked refrigerated sample when compared to cooked sample.
In Vitro Antibacterial Activities of Cochlospermum planchonii Roots Crude Ext...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether of
Cochlospermum planchonii root extracts on some clinical bacterial isolates and reference organisms were
investigated using conventional microbiological and microdilution indicator technique. Phytochemical
screenings were also carried on the extracts. The root extracts of the plant exhibited antibacterial activities
against reference strains and clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii. However, the susceptibility pattern of the bacteria did not
differ significantly from each other (p>0.05). The methanolic root extracts exhibited the highest antibacterial
activity, its minimum inhibitory concentration (MIC) ranging between 1.25 mg/ml and 5.00mg/ml; and its zones
of inhibition diameter on the various test microorganisms ranging between 8mm and 12mm. The petroleum
ether extracts had the weakest antibacterial activity, with minimum inhibitory concentration of 5.00mg/ml and
its zones of inhibition diameter ranging between 4mm and 7mm. The bioactive constituents in the plant were
alkaloids, tannins, saponins, cardiac glycosides, and sterols. The methanolic extracts of root appeared to be
more biologically active than other extracts and may be more useful in treating human infections caused by
these pathogens.
Antifungal Activities and Phytochemical Screening of Xanthium strumariumDheeraj Vasu
ABSTRACT: Antifungal activities and phytochemical screening of Xanthium strumarium (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. Five phytopathogenic fungi: Alternaria brassicae, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Sclerotium rolfsii were tested at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 250 mg/ml) of selected plant extracts. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antifungal activity of extracts was determined by poisoned food technique; and linear mycelium growth reduction (LMGR) percentage was calculated. The distilled water extracts inhibited the growth of fungal mycelium while methanolic extracts completely inhibited (100%) the growth of some selected fungi at higher concentrations. Fusarium oxysporum was the most susceptible fungus while A. brassicae and B. cinerea were the most resistant fungi.
Key words: Antifungal activities, phytochemical screening, Xanthium strumarium, Linear Mycelium Growth Reduction (LMGR)
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on ...researchanimalsciences
Background and Objectives:
This paper reports the toxicity of
Lantana camara
to
developmental stages of the yellow fever mosquito,
Aedes aegypti
. Aqueous extracts
of leaf and seed of the plant were also tested for their effect on the hatchability of
mosquito egg and age at pupation and emergence.
Methods:
Different concentrations of aqueous leaf and seed extract were prepared.
The data of mortality rate were subjected to finney’s method of probit analysis. The
plant was also tested for their effect on the hatchability of mosquito eggs.
Results:
Percent log LC
50
/ 24 h values of the leaf and seed extracts of
L. camara
to
IV instar larvae were 2.25 and 2.25 respectively. Percent hatchability of mosquito eggs
was remarkably reduced when treated with higher concentration of the toxicants.
Extended time of pupation and emergence was observed for the larvae reared in
different concentrations of the plant extract.
Conclusion:
The results suggested that leaf and seed extract of
Lantana camera
possessed remarkable larvicidal, ovicidal, and prolonged time of pupation and adult
emergence against
Aedes aegypti.
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on A...researchanimalsciences
Background and Objectives: This paper reports the toxicity of Lantana camara to developmental stages of the yellow fever mosquito, Aedes aegypti. Aqueous extracts of leaf and seed of the plant were also tested for their effect on the hatchability of mosquito egg and age at pupation and emergence.
Methods: Different concentrations of aqueous leaf and seed extract were prepared. The data of mortality rate were subjected to finney’s method of probit analysis. The plant was also tested for their effect on the hatchability of mosquito eggs.
Results: Percent log LC50 / 24 h values of the leaf and seed extracts of L. camara to IV instar larvae were 2.25 and 2.25 respectively. Percent hatchability of mosquito eggs was remarkably reduced when treated with higher concentration of the toxicants. Extended time of pupation and emergence was observed for the larvae reared in different concentrations of the plant extract.
Conclusion: The results suggested that leaf and seed extract of Lantana camera possessed remarkable larvicidal, ovicidal, and prolonged time of pupation and adult emergence against Aedes aegypti.
Article Citation:
Sathya K, Mohanraj RS, Dhanakkodi B .
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on Aedes aegypti.
Journal of Research in Animal Sciences (2012) 1(2): 040-047.
Full Text:
http://janimalsciences.com/documents/AS0013.pdf
Invitro Evaluation of Antimicrobial Activity of the plant extracts of Elytrar...IJERA Editor
Antimicrobial activity of the aerial parts of the Elytraria acaulis a stem less perennial herb of Acantheceae family has been carried out in the present study. Extracts of the aerial parts of the plant (Stem & Leaves) were prepared in different organic solvents such as n-Hexane, Ethanol, Methanol and Ethyl acetate. All the extracts were analyzed for antimicrobial properties against various pathogenic bacterial infections (Escherichia coli, Klebsiella Species, and Salmonella Typhi). Amongst all the extracts, methanolic extract exhibited significant antimicrobial activity. The crude methanol extracts of leaves showed good inhibitory effects against pathogens. Hence further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications.
Evaluation of Antimicrobial Activity of Excoecaria Agallocha Lijtsrd
Excoecaria agallocha L. is an important medicinal plant inhabited in mangrove regions. Early researches focused on antimicrobial activity of leaves of concerned plant with various solvents among which ethanol, chloroform and methanol were Used. Ella. Sai Kumar "Evaluation of Antimicrobial Activity of Excoecaria Agallocha L" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-3 , April 2020, URL: https://www.ijtsrd.com/papers/ijtsrd30276.pdf Paper Url :https://www.ijtsrd.com/biological-science/microbiology/30276/evaluation-of-antimicrobial-activity-of-excoecaria-agallocha-l/ella-sai-kumar
Antimicrobial activity of herbal productionkarimbscdu
The use of plants in treatment of burns, dermatophytes and infectious diseases is common in traditional medicine. The development of new antimicrobial agents against resistant pathogens is increasing interest. Therefore, the methanolic extracts from different parts of four medicinal plants used locally in folk medicine were evaluated for antimicrobial activity. It was found that most plant extracts studied had antibacterial and antifungal activities. The methanolic extract of leaf of the plant Azadiracta indica, Acacia nilotica and Witania somnifera showed significant antibacterial activity against Bacillus subtilis, Escherchia coli, stphaylocuccus aureus and pseudomonas fluorescence. Azadiracta indica and A.tinolica showed significant antifungal activity against A. flavus, Ziziphus mauritiana. The rhizome extract of curcuma longa showed significant activity against all tested bacteria and showed higher anti fungal activity against Fusarium verticillioides
ABSTRACT- Phylloplane fungi presented on the leaf surface were screened and selected for the assessment of their
potential against Alternaria alternata causing leaf spot disease of Rauwolfia serpentina (Sarapgandha). Among the
antagonists Trichoderma harzianum ISO-2 showed minimum conidial germination. Effect of foliar spray of phylloplane
fungi on the lesion development was also studied. In mist chamber studies seven treatments comprising application of
antagonists in the presence of pathogen proved effective in causing per cent disease reduction by 30-50 % in comparison
with the control. In the field trials Penicillium sublateritium showed the maximum fresh and dry root weight. P. sublateritium
also showed significant percentage disease reduction in comparison to control.
Key words- Alternaria alternata, Foliar spray, Leaf spot, Phylloplane fungi, Rauwolfia serpentina
Biological Efficacy of Quassia Indica (Geratn) Nooteb and Centella Asiatica (...IOSRJPBS
Antimicrobial activity of different extracts of two selected plants, Quassia indica (Geartn) Nooteb and Centella asiatica (L.) Urban were tested against selected strains of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas sps, Bacillus thuringiensis, Klebsiella sps and Serratia sps using agar disc diffusion technique. Petroleum ether, acetone, methanol and water extracts of seed and leaf of Q. indica and leaf of C. asiatica showed moderate to significant antimicrobial activity. Of the four extracts tested, petroleum ether and water fractions showed more activity against all the bacterial strains. The zone of inhibition of the active fractions for the petroleum ether ranged from 8.57 to 12.63 mm and 8.53 to 14.5mm for the water fraction of Q. indica seed and leaf and C. asiatica leaf; and exhibited comparable results with widely used commercial antimicrobial agents (16 to19 mm for Kanamycin and 14 to 17mm for Cefotaxime). The results prove the efficacy of the most active fractions of the selected plant extracts to be used for developing potent antimicrobial formulations.
Effect of environmental pollution on the quality of an edible plant Alternant...Premier Publishers
The present study is the comparative analysis of phytochemical constituents and microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb collected from unpolluted and polluted site. Preliminary phytochemical analysis was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts (unpolluted and polluted site) of A philoxeroides that showed the presence of alkaloids, carbohydrates, saponins, phenols, flavonoids, aminoacids, diterpenes, tannin, terpenoids, protein, steroid, oxalate, coumarin and quinones. The ethanol extract showed higher number of phytochemical constituents when compared to the other extract of unpolluted site. The microbial load is also enumerated in the unpolluted and polluted site. In conclusion, phytochemical analysis revealed the presence of many phytoconstituents in ethanol extract and the microbial load is less in the unpolluted site when compared to the polluted site.
Evaluation of antibacterial activity of some medicinal plants used by Sugali ...researchplantsciences
In the present work an attempt has been made to carry out screening for the preliminary antibacterial activity of different plants used by Sugali tribes of Yerramalis forest. Fifteen plants were selected for preliminary screening for their antibacterial potentiality, The antibacterial activity was done against four bacterial strains, viz., Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli,.The preliminary screening experiment revealed that methanol extracts were more potent than the aqueous extracts. The most susceptible bacteria were K. pneumoniae and the most resistant bacteria were E. coli. Bauhinia racemosa L. exhibited remarkable antibacterial activity.
Article Citation:
Khaleel Basha S, Sudarsanam G, Hari Babu Rao D, Niaz Parveen.
Evaluation of antibacterial activity of some medicinal plants used by Sugali tribe of Yerramalais forest of Andhra Pradesh, India.
Journal of Research in Plant Sciences (2011) 1(1): 027-031.
Full Text:
http://plantsciences.co.in/documents/PS0003.pdf
Trichosporon sp. are widely distributed in nature and can predominantly be found in the environmental substrates, such as soil, birds, vegetables, water, and decomposing wood. These fungi can colonize skin and, less frequently, respiratory and gastrointestinal tracts of humans. Currently, the genus Trichosporon sp. is considered one of the most important emerging causes of invasive infection in immune compromised patients, with T. asahii being the most frequently reported species. In this study, T. asahii was procured from MTCC and its sensitivity was checked against different solvents (Methanol, Ethanol, Acetone, and Chloroform) of Iris ensata, a medicinal plant. Zone of inhibition and MIC were evaluated using Agar well diffusion assay and Tube dilution broth assay. Ketoconazole and Nystatin B were used as positive controls. Phytochemical screening was done to determine the phytochemicals present in the plant. The methanol extract was found to be most effective compared to other solvent extracts and positive controls. Phytochemicals play a major role in this anti Trichosporon activity. Key-words- Yeast infection, Trichosporon, Medicinal plant, Phytochemicals test
ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT EXTRACS AGAINST PLANT PATHOG...IJSIT Editor
The aim of this work was to find an alternative to chemical fungicides currently used in the control
plant pathogenic fungi Rhizoctoniasolani ,ColletotrichummusaeandFusariumoxysporum,. The antifungal
activity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.All
plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID)
against above fungi. The media amended with methanol and recommended fungicide for respective fungal
strain were consider as negative and positive control respectively.Results showed that radial growth in all the
three tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culture
medium used. The test fungi differed in their reaction to the different extracts but on the whole, growth
inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of
the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R.
solani and C.muceawhereas those from wild basil inhibited the growth of the same pathogens by 89%, 84%
and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild
basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet
flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed
60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and
wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the
potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthe...Premier Publishers
The present study analyses the antimicrobial activity and the microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb growing in polluted and unpolluted site. The plants were collected and tested against various Gram positive, Gram negative bacteria and fungi. Antimicrobial activity was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts of aerial parts A. philoxeroides collected from polluted and unpolluted site that showed significant antimicrobial activity against tested bacterial and fungal organisms. The extracts were compared with standards like Amoxicillin for antibacterial activity and Ketoconazole for antifungal activity. The extracts showed remarkable antimicrobial activity as measured from the zone of inhibition and results were comparable with that of standard drugs against the organisms tested. The microbial load is also enumerated in the cooked and cooked refrigerated samples from polluted and unpolluted site. In conclusion, plant extract of A. philoxeroides collected from polluted site showed less antimicrobial activity and higher antimicrobial activity in unpolluted site. The ethanol extract showed higher activity when compared to other extracts. The microbial load is higher in cooked refrigerated sample when compared to cooked sample.
In Vitro Antibacterial Activities of Cochlospermum planchonii Roots Crude Ext...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether of
Cochlospermum planchonii root extracts on some clinical bacterial isolates and reference organisms were
investigated using conventional microbiological and microdilution indicator technique. Phytochemical
screenings were also carried on the extracts. The root extracts of the plant exhibited antibacterial activities
against reference strains and clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii. However, the susceptibility pattern of the bacteria did not
differ significantly from each other (p>0.05). The methanolic root extracts exhibited the highest antibacterial
activity, its minimum inhibitory concentration (MIC) ranging between 1.25 mg/ml and 5.00mg/ml; and its zones
of inhibition diameter on the various test microorganisms ranging between 8mm and 12mm. The petroleum
ether extracts had the weakest antibacterial activity, with minimum inhibitory concentration of 5.00mg/ml and
its zones of inhibition diameter ranging between 4mm and 7mm. The bioactive constituents in the plant were
alkaloids, tannins, saponins, cardiac glycosides, and sterols. The methanolic extracts of root appeared to be
more biologically active than other extracts and may be more useful in treating human infections caused by
these pathogens.
Antifungal Activities and Phytochemical Screening of Xanthium strumariumDheeraj Vasu
ABSTRACT: Antifungal activities and phytochemical screening of Xanthium strumarium (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. Five phytopathogenic fungi: Alternaria brassicae, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Sclerotium rolfsii were tested at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 250 mg/ml) of selected plant extracts. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antifungal activity of extracts was determined by poisoned food technique; and linear mycelium growth reduction (LMGR) percentage was calculated. The distilled water extracts inhibited the growth of fungal mycelium while methanolic extracts completely inhibited (100%) the growth of some selected fungi at higher concentrations. Fusarium oxysporum was the most susceptible fungus while A. brassicae and B. cinerea were the most resistant fungi.
Key words: Antifungal activities, phytochemical screening, Xanthium strumarium, Linear Mycelium Growth Reduction (LMGR)
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Similar to Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and Semi-Field Conditions
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on ...researchanimalsciences
Background and Objectives:
This paper reports the toxicity of
Lantana camara
to
developmental stages of the yellow fever mosquito,
Aedes aegypti
. Aqueous extracts
of leaf and seed of the plant were also tested for their effect on the hatchability of
mosquito egg and age at pupation and emergence.
Methods:
Different concentrations of aqueous leaf and seed extract were prepared.
The data of mortality rate were subjected to finney’s method of probit analysis. The
plant was also tested for their effect on the hatchability of mosquito eggs.
Results:
Percent log LC
50
/ 24 h values of the leaf and seed extracts of
L. camara
to
IV instar larvae were 2.25 and 2.25 respectively. Percent hatchability of mosquito eggs
was remarkably reduced when treated with higher concentration of the toxicants.
Extended time of pupation and emergence was observed for the larvae reared in
different concentrations of the plant extract.
Conclusion:
The results suggested that leaf and seed extract of
Lantana camera
possessed remarkable larvicidal, ovicidal, and prolonged time of pupation and adult
emergence against
Aedes aegypti.
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on A...researchanimalsciences
Background and Objectives: This paper reports the toxicity of Lantana camara to developmental stages of the yellow fever mosquito, Aedes aegypti. Aqueous extracts of leaf and seed of the plant were also tested for their effect on the hatchability of mosquito egg and age at pupation and emergence.
Methods: Different concentrations of aqueous leaf and seed extract were prepared. The data of mortality rate were subjected to finney’s method of probit analysis. The plant was also tested for their effect on the hatchability of mosquito eggs.
Results: Percent log LC50 / 24 h values of the leaf and seed extracts of L. camara to IV instar larvae were 2.25 and 2.25 respectively. Percent hatchability of mosquito eggs was remarkably reduced when treated with higher concentration of the toxicants. Extended time of pupation and emergence was observed for the larvae reared in different concentrations of the plant extract.
Conclusion: The results suggested that leaf and seed extract of Lantana camera possessed remarkable larvicidal, ovicidal, and prolonged time of pupation and adult emergence against Aedes aegypti.
Article Citation:
Sathya K, Mohanraj RS, Dhanakkodi B .
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on Aedes aegypti.
Journal of Research in Animal Sciences (2012) 1(2): 040-047.
Full Text:
http://janimalsciences.com/documents/AS0013.pdf
This survey was conducted on an experimental parcel of the Agronomic High School of the National Polytechnic Institute Félix HOUPHOUET- BOIGNY (ESA-INPHB) of Yamoussoukro (Côte d’Ivoire). It aims to evaluate the insecticidal effect of 2 local plants aqueous extracts on termites (Azadirachta indica and Cassia siamea) dosed at 131.25 g powder/ L compared to a chemical pesticide (Chlorpyrifos ethyl) dosed at 480 g / L. A total of 11 termite species were collected from the plots. The fungus-growers with 6 species are the most dominant group. Four soil-feeder species and one wood-boring species were also collected. Only fungus-growers are present in the areas treated with plant extracts and chlorpyrifos ethyl. It was observed a high abundance of termites in heading stage of the rice growth. In maturity stage, ie 4 months after sowing the efficiency of chlorpyrifos ethyl decreased as plant aqueous extracts remain effective. Among the two tested plants, Azadirachta indica seems to be the most effective compared to Cassia siamea against termites. Chlorpyrifos ethyl could be used as a cure for its swift action on termites while Azadirachta indica could be used preventively and for crops whose destructive action is long term.
Identification and evaluation of antifungal compounds from botanicals for the...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus, Colletotrichum falcatum. In this study, eighteen different botanicals were screened for identifying effective antifungal compound against C. falcatum. Among the plants screened, 15 per cent aqueous leaf extract of Psoralea corylifolia alone inhibited 100 per cent growth of both mycelium as well as spore germination under in vitro conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes viz., Pseudomonas fluorescens, Bacillus megaterium and Gluconacetobacter diazotrophicus which are normally used in sugarcane. The effective plant extracts exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC-MS analysis to identify the bioactive antifungal compound. It revealed the presence of 7H-furo [3,2-G] (1) benzopyran-7-one as main bioactive compound which is thought to be the intermediate of antifungal compound, 8 – methoxypsoralen formed during biosynthesis.
Article Citation:
Rajkumar D and Murugesan R.
Identification and Evaluation of Antifungal Compounds from Botanicals for the Control of Sugarcane Red Rot Pathogen, Colletotrichum falcatum.
Journal of Research in Agriculture (2013) 2(1): 164-172.
Full Text:
http://www.jagri.info/documents/AG0044.pdf
Identification and Evaluation of Antifungal Compounds from Botanicals for th...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus,
Colletotrichum
falcatum
. In this study, eighteen different botanicals were screened for
identifying effective antifungal compound against
C.
falcatum.
Among the plants
screened, 15 per cent aqueous leaf extract of
Psoralea corylifolia
alone inhibited 100
per cent growth of both mycelium as well as spore germination under
in vitro
conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes
viz.
,
Pseudomonas fluorescens
,
Bacillus megaterium
and
Gluconacetobacter
diazotrophicus
which are normally used in sugarcane. The effective plant extracts
exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC
-
MS
analysis to identify the bioactive antifungal compound. It revealed the
presence of
7H
-
furo [3,2
-
G] (1) benzopyran
-
7
-
one as main bioactive compound which is thought to be
the intermediate of antifungal compound, 8
–
methoxypsoralen formed during
biosynthesis.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Effect of Extract of Ailanthus Excelsa on Red Cotton Bug (Dysdercus Cingulatus)IOSR Journals
Botanical pesticides have pesticidal or ovicidal or repellent activities. The development of resistance to existing conventional synthetic pesticides and the increasing public concern over environmental pollution and health hazards created by synthetic pesticides, generate a great need for new types of pest management agent’s advantage with higher activity against the target pests, and lower impact on humans and environmental quality. Repellents are the chemicals which cause an insect to make an oriented movement away from its source. Due to their odoriferous nature, oils of plants have been used for this purpose. Repellency in the present study has been noted visually in the treated insects and the observations recorded have been depicted. The results showed 95% repellency in the treated arms of petridish in fraction of Ailanthus ecxcelsa.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium...researchagriculture
Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic pests in smallholder vegetable production, causing significant yield losses in heavy infestations. Current control strategy focuses on use of synthetic pesticides that consequently lead to decimation of natural enemies, development of insect resistance and resurgence and upset biodiversity. Botanical pesticides have been used widely in smallholder farmers but not much documented literature exists on efficacy of these products. A field trial was done to assess the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized complete block design (RCBD) with five treatments replicated four times. The five treatments used in the experiment were T. vogelii, A. sativum, S. incanum, dimethoate and control. Wingless adult female aphids were inoculated three weeks after transplanting of seedlings. Spraying and data collection were done weekly for four weeks. Data was collected on aphid nymph and adult counts on the third leaf from the aerial plant part of randomly selected plants from each treatment for 24 hours after the application of treatments and total plant fresh weight per each treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on aphid in rape production.
Article Citation:
Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)
Journal of Research in Agriculture (2013) 2(1): 157-163.
Full Text:
http://www.jagri.info/documents/AG0040.pdf
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Alli...researchagriculture
Cabbage aphid (
Brevicoryne brassicae
L.) is one of the most problematic
pests in smallholder vegetable production, causing significant yield losses in heavy
infestations. Current control strategy focuses on use of synthetic pesticides that
consequently lead to decimation of natural enemies, development of insect
resistance and resurgence and upset biodiversity. Botanical pesticides have been used
widely in smallholder farmers but not much documented literature exists on efficacy
of these products. A field trial was done to assess the efficacy of crude aqueous
extracts of
Tephrosia vogelii
,
Allium sativum
and
Solanum incanum
in controlling
Brevicoryne brassicae
in
Brassica napus
production. The trial was laid in a randomized
complete block design (RCBD) with five treatments replicated four times. The five
treatments used in the experiment were
T
.
vogelii
,
A
.
sativum
,
S
.
incanum
,
dimethoate and control. Wingless adult female aphids were inoculated three weeks
after transplanting of seedlings. Spraying and data collection were done weekly for
four weeks. Data was collected on aphid nymph and adult counts on the third leaf
from the aerial plant part of randomly selected plants from each treatment for
24 hours after the application of treatments and total plant fresh weight per each
treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that
T. vogelii
,
S
.
incanum
and
A
.
sativum
aqueous crude extracts have some pesticidal
effects on aphid in rape
production.
Allelopathic effects of weeds extract on seed germination of Trachyspermum co...Innspub Net
Seed germination stage is one of the most basic growth stages in medicinal plants that may be adversely affected by the chemicals released from other plants in the soil environment. The present study was performed to investigate the allelopathic effects of weeds, including (Xanthium strumarium, Amaranthus retroflexus and Chenopodium album) as common weeds in medicinal plant's farms, on seed germination parameters, total dry weight and allometry coefficient of Trachyspermum copticum L., Foeniculum vulgare and Cuminum cyminum L. in laboratory conditions. Treatments included extracts of all organs of three weed species, applied on the seeds of the medicinal plants at three concentrations of zero (distilled water), 50 and 100% by volume. The results showed that the extracts of mentioned weed species had a significant inhibitory effect on germination characteristics of the studied medicinal plants. The greatest negative impact on seed germination was recorded for the extract of Amaranthus retroflexus, but Trachyspermum copticum L. showed a better resistance to the extract of Amaranthus retroflexus as compared to Foeniculum vulgare and Cuminum cyminum. Get the full articles at: http://bit.ly/1NaHI5u
Twice repeated experiment was carried out under laboratory conditions to investigate the allelopathic effects of aqueous extracts of Parthenium hysterophorus L. shoot (stem + branch) and leaf, at 0, 5, 10 and 15 g L-1 (w/v) concentrations on maize (Zea mays L.) seed germination, seedling growth (shoot and root length) and biomass production. The treatments were laid out in completely randomized design with the factorial arrangement in four replications. Result indicated that the highest germination percentage (98.75%) was recorded from control whereas the lowest (43.75 %) was from stem extract at15 g L-1 concentration level. Similar trend was also observed by leaf extract. Root and shoot length of maize crop was reduced by 91.4 % and 70.8% by 85.6 and 35.8% leaf extracts and stem extracts respectively hence the roots were more sensitive to allelopathic effect than shoot. Extract of both leaf and stem at 15 g L-1 strongly reduced fresh and dry biomass of the maize seedling. The highest (100,100 %) tolerance index was recorded from control whereas, the lowest (8.62, 14.74%) was recorded from leaf and stem extract at 15 g L-1 concentration level respectively. Leaf aqueous extract showed more phytotoxic effect (91.37 %) than stem extracts (85.25) at 15 g L-1 whereas was the minimum recorded in control (0.0%). 15 g L-1 minimum value of vigor index (200, 539.2) and higher inhibition potentials (62.5 and 55.78%) were recorded from leaf and stem respectively. therefore allelopathic effect by the parthenium extracts is concentration-dependent manner. Leaf extract had shown highest inhibition potential followed by the stem.
The Potential of Heliotropium indicum and Chili Pepper Extracts to Control Af...Premier Publishers
The experiment examined the potential of some organic extracts to control African army worm on maize plantations. The experiment was laid out in a randomized complete block design (RCBD) consisting of four treatments each replicated four times. Treatments consisted of extracts from the leaves of Heliotropium indicum (HE), extract from dried chilli pepper (CPE), combination of H.indicum with dried chilli pepper extract (HE + CPE) and Cypermethrine (CP) insecticide incorporated in the study for comparison with the botanicals and the untreated check as control (CL). A susceptible and improved maize variety SAMARU 38 was used for the experiment. Data were collected on the following parameters for each treatment: Number of plants infested before and after treatment, percentage damage before and after treatment, total damage after treatment, percentage level of infestation after treatment, and percentage yield increase. All treatments at different plant growth stages reduced the number of holes on the maize plant and these values were significantly different from that of the untreated control plot. Field results indicated that extract of H. indicum, chili pepper and their combinations were effective in controlling army worm. Grain yield in extracts of H.indicum were higher than monocrotophos treatments and the untreated check. Incorporating the plant extracts treatments into sustainable maize production will help to improve the crop yield in resource-poor-farmers field, as well as increase monetary returns for their investment without altering the eco system.
STUDY OF FUNGAL, BACTERIAL AND ACTINOMYCETES POPULATION IN TENDU LEAF LITTER ...Dr Dama
STUDY OF FUNGAL, BACTERIAL AND ACTINOMYCETES POPULATION IN TENDU LEAF LITTER VERMICOMPOST IN COMPARISON WITH PRESS MUDCAKE.
Mushan L.C*. Rao K.R.**, Shagalolu V.V*. and Dama L.B*@.
Similar to Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and Semi-Field Conditions (20)
Evaluation of Agro-morphological Performances of Hybrid Varieties of Chili Pe...Premier Publishers
In Benin, chilli pepper is a widely consumed as vegetable whose production requires the use of performant varieties. This work assessed, at Parakou and Malanville, the performance of six F1 hybrids of chilli including five imported (Laali, Laser, Nandi, Kranti, Nandita) and one local (De cayenne), in completely randomized block design at four replications and 15 plants per elementary plot. Agro-morphological data were collected and submitted to analysis of variance and factor analysis of mixed data. The results showed the effects of variety, location and their interactions were highly significant for most of the growth, earliness and yield traits. Imported hybrid varieties showed the best performances compared to the local one. Multivariate analysis revealed that 'De cayenne' was earlier, short in size, thin-stemmed, red fruits and less yielding (≈ 1 t.ha-1). The imported hybrids LaaliF1 and KrantiF1 were of strong vegetative vigor, more yielding (> 6 t.ha-1) by developing larger, long and hard fruits. Other hybrids showed intermediate performances. This study highlighted the importance of imported hybrids in improving yield and preservation of chili fruits. However, stability and adaptation analyses to local conditions are necessary for their adoption.
An Empirical Approach for the Variation in Capital Market Price Changes Premier Publishers
The chances of an investor in the stock market depends mainly on some certain decisions in respect to equilibrium prices, which is the condition of a system competing favorably and effectively. This paper considered a stochastic model which was latter transformed to non-linear ordinary differential equation where stock volatility was used as a key parameter. The analytical solution was obtained which determined the equilibrium prices. A theorem was developed and proved to show that the proposed mathematical model follows a normal distribution since it has a symmetric property. Finally, graphical results were presented and the effects of the relevant parameters were discussed.
Influence of Nitrogen and Spacing on Growth and Yield of Chia (Salvia hispani...Premier Publishers
Chia is an emerging cash crop in Kenya and its production is inhibited by lack of agronomic management information. A field experiment was conducted in February-June and May-August 2021, to determine the influence of nitrogen and spacing on growth and yield of Chia. A randomized complete block design with a split plot arrangement was used with four nitrogen rates as the main plots (0, 40, 80, 120 kg N ha-1) and three spacing (30 cm x 15 cm (s1), 30 cm x 30 cm (s2), 50 cm x 50 cm (s3)). Application of 120 kg N ha-1 significantly increased (p≤0.05) vegetative growth and seed yield of Chia. Stem height, branches, stem diameter and leaves increased by 23-28%, 11-13%, 43-55% and 59-88% respectively. Spacing s3 significantly increased (p≤0.05) vegetative growth. An increase of 27-74%, 36-45% and 73-107% was recorded in number of leaves, stem diameter and dry weight, respectively. Chia yield per plant was significantly higher (p≤0.05) in s3. However, when expressed per unit area, s1 significantly produced higher yields. The study recommends 120 kg N ha-1 or higher nitrogen rates and a closer spacing of 15 cm x 30 cm as the best option for Chia production in Kenya.
Enhancing Social Capital During the Pandemic: A Case of the Rural Women in Bu...Premier Publishers
Social capital plays an essential role in empowering people for social and economic change even during the pandemic. A livelihood project of the government was implemented among the members of a women’s association of a disadvantaged upland community in Bukidnon province, Southern Philippines for inclusive development. This study was conducted to determine the influence of some socio-economic attributes and the change in the knowledge level on the social capital of the rural women amidst the pandemic. The activities of the project were implemented considering the health protocols imposed by the government during the health crisis. The findings revealed that the trainings conducted resulted to a positive change in the knowledge level among the rural women. This facilitated the production of vegetables for their households and generated additional income very necessary during the pandemic especially that other economic activities were hindered. Similarly, there was a significant increase in the social capital of the rural women during the last two years. The main occupation, sources of income and their ethnicity significantly influenced the social capital of the rural women. The rural development workers and policymakers must consider the social capital of the group in the implementation of poverty alleviation programs.
Impact of Provision of Litigation Supports through Forensic Investigations on...Premier Publishers
This paper presents an argument through the fraud triangle theory that the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases is adequate for effective prosecution of perpetrators as well as corporate fraud prevention. To support this argument, this study operationalized provision of litigation supports through forensic audit and investigations, data mining for trends and patterns, and fraud data collection and preparation. A sample of 500 respondents was drawn from the population of professional accountants and legal practitioners in Nigeria. Questionnaire was used as the instrument for data collection and this was mailed to the respective respondents. Resulting responses were analyzed using the OLS multiple regression techniques via the SPSS statistical software. The results reveal that the provision of litigation supports through forensic audits and investigations, fraud data mining for trends and patterns and fraud data collection and preparation for court proceedings have a positive and significant impact on corporate fraud prevention in Nigeria. This study therefore recommends that regulators should promote the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases in publicly listed firms in Nigeria, as this will help provide reports that are acceptable in court proceedings.
Improving the Efficiency of Ratio Estimators by Calibration WeightingsPremier Publishers
It is observed that the performances of most improved ratio estimators depend on some optimality conditions that need to be satisfied to guarantee better estimator. This paper develops a new approach to ratio estimation that produces a more efficient class of ratio estimators that do not depend on any optimality conditions for optimum performance using calibration weightings. The relative performances of the proposed calibration ratio estimators are compared with a corresponding global [Generalized Regression (GREG)] estimator. Results of analysis showed that the proposed calibration ratio estimators are substantially superior to the traditional GREG-estimator with relatively small bias, mean square error, average length of confidence interval and coverage probability. In general, the proposed calibration ratio estimators are more efficient than all existing estimators considered in the study.
Urban Liveability in the Context of Sustainable Development: A Perspective fr...Premier Publishers
Urbanization and quality of urban life are mutually related and however it varies geographically and regionally. With unprecedented growth of urban centres, challenge against urban development is more in terms of how to enhance quality of urban life and liveability. Making sense of and measuring urban liveability of urban places has become a crucial step in the context of sustainable development paradigm. Geographical regions depict variations in nature of urban development and consequently level of urban liveability. The coastal regain of West Bengal faces unusual challenges caused by increasing urbanization, uncontrolled growth, and expansion of economic activities like tourism and changing environmental quality. The present study offers a perspective on urban liveability of urban places located in coastal region comprising of Purba Medinipur and South 24 Parganas districts. The study uses the liveability standards covering four major pillars- institutional, social, economic and physical and their indicators. This leads to develop a City Liveability Index to rank urban places of the region, higher the index values better the urban liveability. The data for the purpose is collected from various secondary sources. Study finds that the eastern coastal region of the country covering state of West Bengal depicts variations in index of liveability determined by physical, economic, social and institutional indicators.
Transcript Level of Genes Involved in “Rebaudioside A” Biosynthesis Pathway u...Premier Publishers
Stevia rebaudiana Bertoni is a plant which has recently been used widely as a sweetener. This medicinal plant has some components such as diterpenoid glycosides called steviol glycosides [SGs]. Rebaudioside A is a diterpenoid steviol glycoside which is 300 times sweeter than table sugar. This study was done to investigate the effect of GA3 (50 mg/L) on the expression of 14 genes involved in Rebaudioside A biosynthesis pathway in Stevia rebaudiana under in vitro conditions. The expression of DXS remarkably decreased by day 3. Also, probably because of the negative feedback of GA3 on MEP-drived isoprenes, GGDS transcript level reached its lowest amount after GA3 treatment. The abundance of DXR, CMS, CMK, MCS, and CDPS transcripts showed a significant increase at various days after this treatment. A significant drop in the expression levels of KS and UGT85C2 is detected during the first day. However, expression changes of HDR and KD were not remarkable. Results revealed that the level of transcript of UGT74G1 and UGT76G1 up regulated significantly 4 and 2 times higher than control, respectively. However, more research needs to shed more light on the mechanism of GA3 on gene expression of MEP pathway.
Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Mor...Premier Publishers
Information on genetic variability for biochemical characters is a prerequisite for improvement of tea quality. Thirteen introduced tea clones characterized with objective; assessing tea clones based on morphological characters at Melko and Gera research stations. The study was conducted during 2017/18 cropping season on experimental plots in RCBD with three replications. Data recorded on morphological traits like days from pruning to harvest, height to first branch, stem diameter, leaf serration density, leaf length, leaf width, leaf size, petiole length, leaf ratio, internode length, shoot length, number of shoot, canopy diameter, hundred shoot weight, fresh leaf yield per tree. Cluster analysis of morphological trait grouped into four clusters indicated, the existence of divergence among the tested clones. The maximum inter-cluster distance was between clusters I and IV (35.27) while the minimum inter cluster distance was observed between clusters I and II (7.8).Principal components analysis showed that the first five principal components with eigenvalues greater than one accounted 86.45% for 15 morphological traits. Generally, the study indicated presence of variability for several morphological traits. However, high morphological variation between clones is not a guarantee for a high genetic variation; therefore, molecular studies need to be considered as complementary to biochemical studies.
Causes, Consequences and Remedies of Juvenile Delinquency in the Context of S...Premier Publishers
This research work was designed to examine nature of juvenile offences committed by juveniles, causes of juvenile delinquency, consequences of juvenile delinquency and remedies for juvenile delinquency in the context of Sub-Saharan Africa with specific reference to Eritrea. Left unchecked, juvenile delinquents on the streets engage in petty theft, take alcohol or drugs, rape women, rob people at night involve themselves in criminal gangs and threaten the public at night. To shed light on the problem of juvenile delinquency in the Sub-Saharan region data was collected through primary and secondary sources. A sample size of 70 juvenile delinquents was selected from among 112 juvenile delinquents in remand at the Asmara Juvenile Rehabilitation Center in the Eritrean capital. The study was carried out through coded self-administered questionnaires administered to a sample of 70 juvenile delinquents. The survey evidence indicates that the majority of the juvenile respondents come either from families constructed by unmarried couples or separated or divorced parents where largely the father is missing in the home or dead. The findings also indicate that children born out of wedlock, families led by single mothers, lack of fatherly role models, poor parental-child relationships and negative peer group influence as dominant causes of juvenile infractions. The implication is that broken and stressed families are highly likely to be the breeding grounds for juvenile delinquency. The survey evidence indicates that stealing, truancy or absenteeism from school, rowdy or unruly behavior at school, free-riding in public transportation, damaging the book of fellow students and beating other young persons are the most common forms of juvenile offenses. It is therefore, recommended that parents and guardians should exercise proper parental supervision and give adequate care to transmit positive societal values to children. In addition, the government, the police, prosecution and courts, non-government organizations, parents, teachers, religious leaders, education administrators and other stakeholders should develop a child justice system that strives to prevent children from entering deeper into the criminal justice process.
The Knowledge of and Attitude to and Beliefs about Causes and Treatments of M...Premier Publishers
Stigma and discrimination associated with mental illness are a common occurrence in the Sub-Saharan region including Eritrea. Numerous studies from Sub-Saharan Africa suggest that stigma and discrimination are major problems in the community, with negative attitudes and behavior towards people with mental illness being widespread. In order to assess the whether such negative attitudes persist in the context of Eritrea this study explored the knowledge and perceptions of 90 Eritrean university students at the College of Business and Economics, the University of Asmara regarding the causes and remedies of mental illness A qualitative method involving coded self-administered questionnaires administered to a sample of 90 university students to collecting data at the end of 2019. The survey evidence points that almost 50% of the respondents had contact with a mentally ill person suggesting that the significant number of the respondents experienced a first-hand encounter and knowledge of mental illness in their family and community. The findings show an overall greater science-based understanding of the causes of mental illness to be followed by recommended psychiatric treatments. The survey evidence indicates that the top three leading causes of mental illness in the context of Eritrea according to the respondents are brain disease (76%), bad events in the life of the mentally ill person (66%) and substance abuse or alcohol taking, smoking, taking drugs like hashish. (54%). The majority of the respondents have a very sympathetic and positive outlook towards mentally ill persons suggesting that mentally illness does not simply affect a chosen individual rather it can happen to anybody regardless of economic class, social status, ethnicity race and religion. Medical interventions cited by the majority of the respondents as being effective treatments for mental illness centered on the idea that hospitals and clinics for treatment and even cures for psychiatric disease. Changing perceptions of mental illnesses in Eritrea that paralleled the very caring and sympathetic attitudes of the sample university students would require raising public awareness regarding mental illness through education, using the mass media to raise public awareness, integrating mental health into the primary health care system, decentralizing mental health care services to increase access to treatment and providing affordable service to maintain positive treatment outcomes.
Effect of Phosphorus and Zinc on the Growth, Nodulation and Yield of Soybean ...Premier Publishers
An investigation was carried out at Kogi State University Student Research and Demonstration farm Anyigba during the 2019 wet season to observe the effect of phosphorus and zinc on the growth, nodulation and yield of soybean. The treatments comprised three levels: phosphorus and zinc (0, 30 and 60 kg P2O5/ha; 0, 5 and 10kg Zn/ha) and two varieties TGX 536 – 02D and Samsoy 2. The investigation revealed that application of phosphorus affected growth, nodulation, yield and some yield components of soybean while zinc application, apart from the plant height, which is reduced significantly, had no significant effect on other growth characters, nodulation, yield and yield components. However, it was generally found to decrease most of the characters. Application of 60 kg P2O5/ha gave the highest growth and yield, while 30 kg P2O5/ha gave the highest nodulation. Application of 60 kg P2O5/ha significantly increased yield to 1.9t/ha, which was significantly higher over the control plots, which gave 1.7t/ha. Crude protein and oil contents of the seeds were not significantly affected by phosphorus application but were significantly affected by zinc application, which significantly decreased protein content as its amount an increase from 0 to 10 kg/ha, and significantly increased oil content from 0 to 5kg/ha and decreased it below 5kg/ha. It was also revealed that the two varieties responded similarly to phosphorus and zinc in terms of growth, grain yield and crude protein content of the seeds.
Influence of Harvest Stage on Yield and Yield Components of Orange Fleshed Sw...Premier Publishers
A field experiment was conducted at Adami Tullu Agricultural Research Center in 2018 under rainfed condition with supplementary irrigation to determine the influence of harvest stage on vine yield and tuberous root yield of orange fleshed sweet potato varieties. The experiment consisted of four harvest stages (105, 120, 135 and 150 days after planting) and Kulfo, Tulla and Guntute varieties. A 4 X 3 factorial experiment arranged in randomized complete block design with three replications was used. Interaction of harvest stage and variety significantly influenced above ground fresh biomass, vine length, marketable tuberous root weight per hectare, commercial harvest index and harvest index. The highest mean values of above ground fresh biomass (66.12 t/ha) and marketable tuberous root weight (56.39 t/ha) were produced by Guntute variety harvested at 135 days after planting. Based on the results, it can be recommended that, farmers of the study area can grow Guntute variety by harvesting at 135 days after planting to obtain optimum vine and tuberous root yields.
Performance evaluation of upland rice (Oryza sativa L.) and variability study...Premier Publishers
This study aimed at assessing genetic variability and to evaluate the performance of 13 improved upland rice varieties for yield and its components based on morphological traits. The field experiment was conducted using a randomized block design at Guraferda and Gimbo districts in the 2019 main cropping season. The analysis of variance (ANOVA) over the two locations revealed significant differences (p≤ 0.05) among varieties for days to 50% heading, days to 85% maturity, panicle length, thousand-grain weight, and grain yield. Similarly, the ANOVA for variety by location interactions depicted significant differences among the tested varieties for days to 50% heading, days to 85% maturity, and thousand-grain weight. High heritability was obtained from days to heading (88.5%), panicle length (85.0%), and grain yield (85.2%), which indicates these traits can be easily improved through selection. High to medium broad sense heritability and genetic advance as percentage of the mean for days to heading, thousand-grain weight, and grain yield indicates a good opportunity for improvement through selection using their phenotypic performance. This is mainly due to the high role of additive gene action in the expression of such traits. This study confirmed the presence of variability among varieties for most of the studied traits, which will create an opportunity for breeders to improve rice yield and other attributes.
Response of Hot Pepper (Capsicum Annuum L.) to Deficit Irrigation in Bennatse...Premier Publishers
This study was conducted at Enchete kebele in Benna-Tsemay Woreda, South Omo Zone to evaluate the response of hot pepper to deficit irrigation on yield and water productivity under furrow irrigation system. The experiment comprised four treatments (100 % of ETc, 85% of ETc, 70 % of ETc and 50% of ETc), respectively. The experiment was laid out in RCBD and replicated four times. The two years combined yield results indicated that, the maximum total yield (20.38 t/ha) was obtained from 100% ETc while minimum yield (12.92 t/ha) was obtained from 50% of ETc deficit irrigation level. The highest WUE 5.22 kg/ha mm-1 was obtained from 50% of ETc. Treatment of 100% ETc irrigation application had highest benefit cost ratio (4.5) than all others treatments. Applying 50% of ETc reduce the yield by 37% when compared to 100 % ETc. Accordingly, to achieve maximum hot pepper yield in areas where water is not scarce, applying 100% ETc irrigation water application level throughout whole growing season under furrow irrigation system is recommended. But, in the study area water scarcity is the major limiting factor for crop production. So, it is possible to get better yield and water productivity of hot pepper when we apply 85% ETc irrigation water throughout growing season under furrow irrigation system.
Harnessing the Power of Agricultural Waste: A Study of Sabo Market, Ikorodu, ...Premier Publishers
Nigeria is still burdened with huge responsibilities of waste disposal because the potential for benefits of proper waste management is yet to be harnessed. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. It is estimated that about 998 million tons of agricultural waste is produced yearly in the country with organic wastes amounting to 80 percent of the total solid wastes. This can be categorized into biodegradable and non-biodegradable wastes. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. The Sabo market was treated as a study case with the adoption of in-depth examinations of the facility, animals and products for sale and waste generated. A combination of experimental, interviews (qualitative) and design simulation (for final phase) was adopted to extract, verify and analyse the data generated from the study. Animal waste samples were subjected to compositional and fibre analysis with results showing that the sample has high potency for biogas production. Biodegradable Wastes are human and animal excreta, agricultural and all degradable wastes. Availability of high quantity of waste generated being organic in Sabo market allows the use of anaerobic digestion to be proposed as a waste to energy technology due to its feasibility for conversion of moist biodegradable wastes into biogas. The study found that at peak supply period during the Islamic festivities, a conservative 300tonnes of animal waste is generated during the week which translates to over 800kilowatts of electricity.
Influence of Conferences and Job Rotation on Job Productivity of Library Staf...Premier Publishers
The general purpose of this study is to investigate the influence of conferences and job rotation on job productivity of library staff in tertiary institutions in Imo State, Nigeria. The survey research design was used for this study using questionnaire as an instrument for data collection. This study covered the entire population of 661. Out of these, 501 copies of the questionnaire representing 75.8% were duly completed and returned for analysis. Student’s t-test was used to analyze the research questions. The finding showed that conferences had no significant influence on the job productivity of library staff in tertiary institutions in Imo State, Nigeria (F cal= 7.86; t-vale =6.177; p >0.005). Finding also showed that job rotation significantly influences job productivity of library staff in tertiary institutions in Imo State, Nigeria (F-cal value= 18.65; t-value = 16.225; P<0.05). This study recommended that, government should ensure that library staff participate in conferences with themes and topics that are relevant to the job they perform and also ensure that there should be proper evaluation and feedback mechanism which aimed to ensuring control and minimize abuse of their development opportunities. Again, there should be written statement of objectives in order to sustain job rotation programmes. Also, that training and development needs of library staff must be identified and analyzed before embarking on job rotation processes as this would help to build skills, competences, specialization and high job productivity.
Scanning Electron Microscopic Structure and Composition of Urinary Calculi of...Premier Publishers
Microscopic examination of urine samples collected from geriatric dogs revealed increased numbers of erythrocytes, leucocytes, epithelial cells and pus cells along with casts, bacteria, spermatozoa and crystals of various shapes. Among the different crystals, triple phosphate or struvite were predominant, followed by calcium oxalate dihydrate, calcium oxalate monohydrate and ammonium urate or biurate. The struvite crystals were, coffin-lid shape and while calcium oxalate dihydrate were octahedron or envelope and monohydrate crystals demonstrated “picket fence” and “dumbbell” and “hemp seed” appearance. Brown or yellow-brown spherical bodies with irregular borders with thorn-apple appearance were shown by ammonium urate or biurate crystals. SEM aspects of magnesium ammonium phosphate crystals revealed perpendicular columnar strata, few with scattered hexa or octa-hedral coffin-lid shaped crystals and calcium phosphate crystals were like cracked eggshells. Presence of wavy phases with sundry areas (uric acid), picket fence (calcium oxalate monohydrate) and typical envelope (calcium oxalate dehydrate) were electron microscopic appearance of various crystals.
Gentrification and its Effects on Minority Communities – A Comparative Case S...Premier Publishers
This paper does a comparative analysis of four global cities and their minority districts which have been experiencing the same structural pressure of gentrification. The main contribution of this paper is providing a detailed comparison of four micro geographies worldwide and the impacts of gentrification on them: Barrio Logan in San Diego, Bo-Kaap in Cape Town, the Mission District in San Francisco, and the Rudolfsheim-Fünfhaus District in Vienna. All four cities have been experiencing the displacement of minority communities due to increases in property values. These cities were chosen because their governments enacted different policies to temper the gentrification process. It was found that cities which implemented social housing and cultural inclusionary policies were more successful in maintaining the cultural and demographic make-up of the districts.
Oil and Fatty Acid Composition Analysis of Ethiopian Mustard (Brasicacarinata...Premier Publishers
The experiments was conducted at Holetta Agricultural Research Center, to analyze forty nine Ethiopian Mustard land races for oil and fatty acid composition traits The experiment was carried out in a simple lattice design. The analysis of variance showed that there were highly significant differences among genotypes for all oil and fatty acid traits compared. The significant difference indicates the existence of genetic variability among the land races which is important for improvement
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
Normal Labour/ Stages of Labour/ Mechanism of LabourWasim Ak
Normal labor is also termed spontaneous labor, defined as the natural physiological process through which the fetus, placenta, and membranes are expelled from the uterus through the birth canal at term (37 to 42 weeks
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and Semi-Field Conditions
2. Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and
Semi-Field Conditions
Kassie WB 136
MATERIALS AND METHODS
The study Design
Laboratory and semi-field experiments were arranged as
Complete Randomized Design (CRD) and Complete
Randomized Block Design (CRBD) respectively, with three
replications per treatment for each botanical extracts
concentration levels. The same batch of C. formosanus
workers for both laboratory and semi-field experiments
was chosen and collected from newly termite infested field
(February-May, 2017) by bucket traps. All botanical
extracts effectiveness was evaluated against workers by
comparing from standard check (Chloropyrifos 48% E. C)
and an untreated check (water).
Collection of plant materials
For efficacy test four locally available botanicals (Table 1)
were selected based on cultural used information and
preliminary study. They collected from Debre Markos town
and surrounding from January 21st to 25th in 2017.
Plant materials were brought into the laboratory by
polyethylene bags. Identification of botanical species has
been done by expert. Voucher specimens of the botanical
species were deposited in herbarium at Debre Markos
University, Ethiopia.
Table 1: List of plant materials tested against C.
formosanus termite workers.
Scientific name Family name Common
name
Part
used
Brassica nigra Brassicaceae Black
Mustard
seeds
Acokanthera
schimperi
Apocynaceae Poison bush Leaves
Croton
macrostachyus
Euphorbiaceae Broad
levead-
croton
Leaves
Rhamnus
prinoides
Rhamnaceae Dogwood Leaves
Preparation of botanical extracts
Collected botanical parts were washed by sterilized water
to avoid dust particles and tiny organisms that are present
on them. After that, they were dried in well-ventilated room
under shade for ten days until completely dry at room
temperature (18-25ºC). Dried form of plant materials was
grinded into powder by using pestle and mortar to obtain
uniform fine powder and stored in separate plastic
containers. Sensitive balance was used to measure 5g,
10g and 15g portion of each grinded fine powder. Each
grinded botanical powder was soaked in 100 ml of water
to obtain three concentration levels (5, 10 and 15 w/v)
weight of powder/volume of water, then shacked for five
minutes to make homogeneous. After stay two days, each
solution supernatant was filtered by Whatman (No.1) to
remove impurities.
Filtration has been done repeatedly to obtain maximum
amount of the extract. Then, 25ml extract solution was
taken and applied independently for each treatment.
Chloropyrifos 48% Emuisifiable Concentrate was diluted in
water based on the recommended field application rate. In
all experiments, the same volume (25ml) of Chlorpyrifos
48% E.C. and water were used as a standard check and
untreated check controls, respectively. The percentage of
extracts varies, when the amount of material used were
transformed, in same water quantity as described (Sahay
et al., 2014).
Efficacy of selected botanical extracts against
Coptotermes formusanus under laboratory condition
Laboratory experiment was done repeatedly to check the
accuracy. Bioassays were carried out to determine the
mortality of Formosan workers by each botanical
termiticides activities. For laboratory test, 42 cups were
labeled and arranged in carton box (120cm x 80cm x
40cm), which was inspected for maintenance of required
moisture level. The volume of each container was 1 liter.
Then, a piece of moistened Eucalyptus globules wood was
added as food source and five gram soil to maintain
moisture level were placed for survival of termites in each
prepared cups. Immediately, 20 workers were counted and
introduced in each 42 prepared cups carefully. For
acclimatization of termites, laboratory room temperature
(20-28o
c) was controlled by using 100 watt electric bulb.
Moisture also adjusted by added drops of water in each
cups to create optimum humidity. The box had covered by
sake that able to exchange of air for them and stay for five
days to check their adaptation in the laboratory. During
application time, from each prepared filtered solution 25ml
extract was sprayed in each cup to test the efficacy.
Mortality of termites was recorded carefully and accurately
after 24, 48 and 72 hours of exposure. Percentage of
mortality has been calculated by Abbott, (1925) Equation.
Efficacy of selected botanical extracts against
Coptotermes formusanus under semi-field condition
Semi-field experiment site was selected at Debre Markos
University, which is protected and was not treated by
pesticides. Similar to laboratory experiment, totally 42
prepared containers were inserted 25cm into the ground.
Then, termite preferred wood and 5 gram soil were added
to each container to serve as food and to keep moisture
respectively.
After that, 20 worker Formosan termites were counted and
introduced in each container. Every cup was covered by
piece of sake separately to prevent direct sun light and
other organisms that can enter to the treatment. After 30
minutes, 25ml filtered solution was sprayed in each
experimental cups to test the efficacy. In semi-field
3. Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and
Semi-Field Conditions
Int. J. Entomol. Nematol. 137
experiment, mortality was counted at evening time (after
11pm) to protect termites from direct sunlight. Similar to
laboratory experiment, the temperature of semi-field was
recorded at 20-24o
C. Mortality of termites was recorded
after 24, 48 and 72 hours of exposure.
Data Analysis
Data was subjected to one-way of analysis of variance
(ANOVA) by using SPSS version 20 software to determine
statistically significant differences among treatments.
Significant differences mean were separated by using
Tukey’s studentized range test (α =0.05). The lethal
concentration (LC50) and (LC90) were analyzed by probit
analysis to determine botanical extracts toxicity. 50% and
90% mortality were calculated after 24, 48 and 72 hours
both under laboratory and semi-field conditions.
RESULTS AND DISCUSSION
Evaluation of the efficacy of botanical extracts under
laboratory conditions
Effectiveness of botanical extracts on Formosan workers
under laboratory condition represented in Table 2. As
indicated in Table 2, at 5 w/v botanical extracts
concentration level after 24 hours exposure highest and
lowest mortality were recorded from B. ngra and R.
prinoides respectively under laboratory condition.
Similarly, after 48 hours exposure of time interval
maximum termite mortality (100%) also was recorded from
standard check.
At 5 w/v botanical extracts concentration level, after 48
hours exposure mean mortality of termites was increased
as 66.67, 33.33, 10.53 and 23.33 mean mortality of
termites were recorded from B. ngra, A. schmperi, C.
macrostachyus and R. prinoides respectively. At 5 w/v
concentration level of botanical extracts, the highest
(78.33) and the least (26.67) mean mortality of termites
under laboratory condition were registered from B. nigra
and C. macrostachyus respectively, after 72 hours
exposure time.
Mean mortality of termite (96.67%) by B. nigra, (76.67)
mortality by A. schmperi (40.00)% by C. macrostachyus
and 73.33 by R. prinoides were recorded under laboratory
condition after 72 hours at 10 w/v concentration. There
was highly significant difference (P<0.0001), among the
different treatments at different time intervals (24, 48 and
72 hours) due to the potency (termicidal effect) of botanical
extracts. There was no significant difference among B.
nigra at 5 w/v, A. schmperi and R. prinoides at 10 w/v
concentration levels after all (24, 48 and 72 hours of
exposure time as indicated in Table 2.
Maximum termite mortality (100%) from Chloropyrifos
followed by non-significantly difference 10 and 15 w/v
concentration of B. nigra and 15 w/v concentration of A.
schmperi after 72 hours exposure under laboratory
condition. As indicated in Table 2, 61.67 and 88.33 mean
mortality of termites were recorded from C. macrostachyus
and R. prinoides respectively at 15 w/v concentration level
after 72 hours exposure of time.
Table 2: Mean mortality ± SE of C. formosanus workers by botanical extracts under laboratory.
Treatments Concentration
(w/v)
Mean mortality ± SE over time (hours) exposure
24 hours 48 hours 72 hours
Brassica nigra 5 53.33 ± 1.67d 66.67 ± 1.67d 78.33 ± 1.67c
10 86.67 ± 1.67b 90.00 ± 0.00b 96.67 ± 1.67a
15 100.00 ± 0.00a 100.00 ± 0.00a 100.00 ± 0.00a
Acokanthera schmperi 5 21.67 ± 1.67g 33.33 ± 2.89f 41.67 ± 1.67e
10 53.33 ± 3.33d 63.33 ± 1.67d 76.67 ± 1.67c
15 91.67± 2.89b 100.00±0.00a 100.00 ± 0.00a
Croton macrostachyus 5 8.33 ± 1.67h 18.33 ± 2.89g 26.67 ± 1.67f
10 30.00 ± 2.89f 36.67± 1.67f 40.00 ± 0.00e
15 43.67 ± 1.67e 53.33 ± 3.33e 61.67 ± 2.89d
Rhamnut prinoides 5 11.67 ± 1.67h 23.33 ± 1.67g 31.67 ± 1.67f
10 56.67 ± 1.67d 65.00 ± 0.00d 73.33 ± 1.67c
15 78.33 ± 1.67c 83.33 ± 1.67c 88.33 ± 1.67b
Chloropyrifos 48% E.C 25ml 100.00 ± 0.00a 100.00 ± 0.00a 100.00 ± 0.00a
Untreated check (water) 25ml 0.00 ± 0.00i 0.00± 0.00h 0.00± 0.00g
CV (%) 5.30 3.67 3.54
P-value <0.0001 <0.0001 <0.0001
Means within a column followed by the same letter are not significantly different (Tukey Student Test (HSD) at p=0.05).
Key: SE=Systematic Error, w/v= weight of powder/volume of water.
4. Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and
Semi-Field Conditions
Kassie WB 138
Evaluation of the efficacy of botanical extracts under
semi-field condition
The result of experiments conducted to evaluate
effectiveness of four locally available botanical insecticides
on Formosan termite workers at different concentrations
under semi-field condition as represented in Table 3.
There was a significant difference among the treatments.
As indicated in (Table 3), at 15 w/v concentration level
after 72 hours exposure time, the highest mean termite
mortality (100%) recorded from B. nigra and A. schmperi
that without significantly difference from Chloropyrifos 48%
E.C.
Table 3: Mean mortality ± SE of C. formosanus workers by botanical extracts under Semi-field condition.
Treatments Concentration
(w/v)
Mean mortality ± SE over time (hours) exposure
24 hours 48 hours 72 hours
Brassica nigra 5 38.33± 1.67e 48.33 ± 2.89de 61.67± 1.67d
10 73.33 ± 3.33bc 85.00 ± 0.00b 93.33 ± 2.89ab
15 100.00 ± 0.00a 100.00 ± 0.00a 100.00 ± 0.00a
Acokanthera schmperi 5 8.67 ± 2.89g 16.67 ± 1.67f 26.67 ± 1.67f
10 40.00± 0.00e 53.33 ± 21.67d 61.67 ± 1.67d
15 76.67 ± 1.67b 86.67± 1.67b 100.00 ± 0.00a
Croton macrostachyus 5 1.67 ± 1.67g 10.00 ± 0.00fg 15.00 ± 0.00g
10 25.00 ± 0.00f 31.33 ± 1.67e 36.67 ± 1.67e
15 56.67 ± 1.67d 66.67 ± 1.67c 73.33 ± 2.89c
Rhamnut prinoides 5 8.33 ± 2.89g 15.00 ± 0.00f 23.33 ± 2.89fg
10 36.67±2.897e 46.67 ± 1.67de 55.00 ± 0.00de
15 66.67± 1.67c 76.67 ± 1.67bc 88.33 ± 2.89b
Chloropyrifos 48% E.C 25ml 100.00 ± 0.00a 100.00 ± 0.00a 100.00 ± 0.00a
Untreated check (water) 25ml 0.00 ± 0.00g 0.00 ± 0.00g 0.00 ± 0.00h
CV (%) 7.25 6.50 4.78
P-value <0.0001 <0.0001 <0.0001
Mean with in a column followed by the same letter are not different (Tukey Student Test (HSD) at
p= 0.05). Key: SE=Systematic Error, w/v= weight of powder/volume of water
In both laboratory and semi-field conditions, the highest
and least mean mortality of termite was recorded from B.
nigra seeds and C. macrostachyus leaf extracts
respectively. No termite mortality was recorded from
untreated check for both laboratory and semi-field
conditions. Mean mortality of termites in all botanical
extracts concentration levels was also increased based on
exposure time intervals.
Dose response bioassay under laboratory condition
The results of the LC50 and LC90, is indicated in (Table 4).
Minimum concentration required to kill 50% and 90% of the
tested worker Formosan termites were calculated for each
botanicals after 24, 48 and 72 hours exposure of time with
their lower and upper limits. As indicated in (Table 4),
minimum concentration (3.97g/100ml) that used to kill 50%
of tested termites’ recorded from B. nigra after 72 hours.
Maximum concentration (15.31 g/100ml) that used to kill
50% of tested termites’ recorded from C. macrostachyus
after 24 hours exposure.
Table 4: Toxicity analysis of botanical extracts on C. formosanus workers under laboratory condition.
Treatments After 24 hours After 48 hours After 72 hours
LC50 LC90 LC50 LC90 LC50 LC90
B. nigra 5.63
(4.12-6.99)
9.71
(8.15-12.59)
4.86
(3.48-6.31)
8.75
(6.53-9.97)
3.97
(2.08-4.87)
6.87
(5.12-8.23)
A. schmperi 9.20
(7.60-10.91)
14.72
(12.66-18.57)
7.65
(6.15-9.17)
12.45
(10.66-15.77)
6.65
(5.15-8.09)
11.14
(9.45-14.24)
C. macrostachyus 15.31
(12.51-22.50)
24.64
(19.16-43.01)
13.44
(10.90-18.64)
23.15
(18.16-37.66)
11.91
(9.56-15.84)
21.44
(17.04-33.04)
R. prinoides 10.36
(8.66-12.78)
16.42
(14.08-21.05)
8.65
(6.95-10.36)
14.52
(12.42-18.34)
7.98
(6.24-9.69)
13.98
(11.88-17.77)
5. Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and
Semi-Field Conditions
Int. J. Entomol. Nematol. 139
Table 5: Toxicity analysis of botanical extracts on C. formosanus workers under semi field condition.
Treatments After 24 hours After 48 hours After 72 hours
LC50 LC90 LC50 LC90 LC50 LC90
B. nigra 7.00
(5.50-8.47)
11.61
(9.89-14.78)
5.94
(4.48-7.31)
10.06
(8.48-12.99)
5.06
(3.68-6.33)
8.54
(7.12-11.23)
A. schmperi 11.43
(9.76-13.50)
17.29
(14.86-22.41)
9.76
(8.12-11.53)
15.45
(13.30-19.55)
8.05
(6.59-9.54)
12.61
(10.86-15.89)
C. macrostachyus 13.89
(12.07-17.17)
19.95
(16.82-28.59)
12.21
(10.32-14.96)
19.17
(16.05-26.38)
11.06
(9.22-13.40)
17.94
(15.11-23.99)
R. prinoides 12.35
(10.50-15.01)
17.38
(16.04-26.12)
10.82
(9.04-13.00)
17.38
(14.74-22.88)
9.25
(7.57-11.05)
15.20
(12.99-19.37)
Dose response bioassay under Semi-field condition
As indicated in Table 5, B. nigra seed extract showed least
LC50 (5.06) and LC90 (8.54) values than other botanicals
after all 24, 48 and 72 exposure of time. Inversely, the
highest LC50 value (13.89) and LC90 value (19.95) were
recorded from C. macrostachyus extract after 24 hours
exposure of time. Based on the LC50 and LC90 values the
most toxic extract was B. nigra and the least toxic was C.
macrostachyus under both laboratory and semi-field
conditions. The order of toxicity was B. nigra, A. schmperi,
R. prinoides and C. macrostachyus in both Table 4 and 5.
In the present study, tested botanical extracts showed
mortality on the C. formusanus workers. As indicated in
Table 2 and 3, there was highly significant difference
(P<0.0001) between different treatments at different time
of exposure (24, 48 and 72 hours) due to the potency of
botanical extracts at all concentration levels. There was no
mortality of termites observed in the untreated check over
the entire exposure (72 hours) of the experiment.
Maximum mean mortality of termites (100%) was
registered from Chloropyrifos 48% E. C, which highly
significantly differences from botanicals treatments that
cause mortality against C. formosanus workers. This is in
agreement with Tadele Shibru, et al., (2014), reported that
Chloropyrifos 48% E. C gives 100% mortality on
Macroterms Spp. under laboratory condition.
The present experiment showed that, mean mortality of
termites was increased, when the concentration level of
botanicals was increased and time of exposure increased
(Table 2 and 3). Based on the present results, maximum
termite mortality in both laboratory and semi-field
conditions were observed at higher concentrations (15
w/v) after 72 hours exposure. This result is in agreement
with Upadhyay, (2013) and Sattar et al., (2014), who
reported that mortality of termites, were directly proportion
to botanical extract concentrations and exposure time of
treatments.
In the present study, mean mortality (100%) on C.
formosanus workers by positive control was not
significantly different from 15 w/v B. nigra both under
laboratory and semi-field conditions after all time of
exposure and A. schmperi under laboratory condition after
48 and 72 hours exposure time (Table 2). In the current
study, B. nigra aqueous seeds extract (LC50= 5.63 g/100ml
water and LC90=9.71 g/100ml) displayed highest lethal
effect against C. formusanus workers under laboratory
condition after 24 hours exposure.
In others investigation, 70% ethanol B. nigra seeds extract,
possessed good microbial activities (Rajesh and Vikas,
2014). Whereas water extracts of elder flowers of
Sambucas nigra can repel granary weevils (Sitophilus
granarius) at 5, 10 and 15 w/v concentrations in 200 ml
water (Ignatowicz and Wesolowska, 2002). In the present
study, C. formosanus termite mortality was recorded in
seed extract of B. nigra and leaf extracts of A. schmperi,
C. macrostachyus and R. prinoides after 24 hours
exposure under laboratory condition (LC50=5.63, 9.20,
15.31 and 10.36g/100ml) and (LC90= 9.71, 14.72, 24.64
and 16.42g/100 ml water) respectively.
In present experiment, C. macrostachyus leaf extracts at
5, 10 and 15 w/v concentration levels after all time of
exposure showed less toxic effect among all botanical
extracts. This result was in lined with the finding of Tadele
Shiberu et al., (2014) who reported that seed extracts of C.
macrostachys at 25% concentration level was also less
toxic effect on Macroterms Spp. (Isoptera: Termitidae),
even after 5 days under laboratory conditions.
Croton macrostachyus leaf extract of the present study
gave 26.67% and 15.00% mortality on C. formosanus
workers after 72 hours exposure at 5 w/v concentrations
under laboratory and semi-field conditions respectively.
While in the other findings, aqueous leaf extracts of C.
macrostachyus at 5 w/v concentration level achieved
100% mortality on African bollworm, Helicover pagrmiyera
Hubner after 72 hours (Ngussie Lulie and Nagappa, 2012).
CONCLUSION
The present study findings had important implications in
the practical control of Formosan subterranean termite by
using botanical extracts, which are easy to prepare. From
the present study results, it can be concluded that, the
efficacy of botanicals depending on the amount of
concentration and time of application (acting in short,
medium and long period of time after treated). The result
showed that, B. nigra and A. schmperi were more effective
6. Evaluation of Selected Botanical Extracts against Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), Under Laboratory and
Semi-Field Conditions
Kassie WB 140
than other treatments on C. formosanus workers. So,
users use high concentration of effective botanicals for
monitoring or preventing Formosan termite workers in
short period of time. From the above result, it is clear that
all the tested botanical extracts at 15 w/v concentration
level after 72 hours provided more than 50% mortality in
both under laboratory and semi-field conditions. As a
result, B. nigra, A. schmperi, C. macrostachyus and R.
prinoides botanical extracts can be used as an integrated
termite management (IPM) at higher concentration levels.
In conclusion, this study considering the bio-activity of
extracts from seeds of B. nigra, leaves of A. schmperi, C.
macrostachyus and R. prinoides at managing C.
formusanus termite workers in the laboratory and semi-
field could be considered for exploration in the
management of Formosan subterranean termites on the
field.
RECOMMENDATIONS
Aqueous extract of tested botanicals has promising in the
laboratory and under semi field conditions. Therefore, wide
range of field study should be made particularly in areas
where Formosan termite species is highly prevalent to
determine the practical potential of the botanical extracts
by using different solvents like ethanol, methanol, distilled
water, etc. at different concentrations. Further studies
should be made on the evaluation of these plants extract
on other termite species and insect pests on the field. This
study also suggests that active ingredient of botanical
extracts responsible for causing mortality of Formosan
termite workers should be identified and non-target effects
of botanical extracts should be studied.
ACKNOWLEDGMENTS
I would like to express my deepest gratitude to Dr.
Sundarsan Reddey for his consistent comments from
starting to the completion of my work and valuable
assistance in SPSS programs and for identification of
Formosans termite species by using different identification
methods.
REFERENCES
Abbott, W. S. (1925). A method of computing the
effectiveness of insecticides. Journal of Economic
Entomology 18:265-267.
Ahmed, S., Leng, C. and David, O. (2005). Field
Evaluation of Noviflumuron for controlling Asian
Subterranean Termite, Coptotermes Gestroi. University
Putra Malaysia.
Chris, P., Terence, L., Joseph, E. and Thomas, G. (2006).
Subterranean Termites: Their Prevention and Control
in Buildings.
Damalas, C. and Eleftherohorinos, I. (2011). Pesticide
exposures, safety issues and risk assessments of
indicators. International Journal of Environmental
Research of Public Health 8:1402-1419.
Engel, M. and Krishna, K. (2004). Family-group names for
termites. American Museum Novitiates 3432: 1–9.
Horwood, M. and Eldridge, R. (2005). Termites in New
South Wales. Forest Resources Research, 21
Technical Publications.
Ignatowicz, S. and Wesolowska, B. (2002). Potential of
common herbs as grain protectants: repellent effects of
herb extracts on the Granary Weevil, Sitophilus
granaries. Poland. Proceeding of the 6th International
conference on Stored Product protection 2:790-794.
Isman, M.B. (2006). Botanical insecticides, detergents and
repellents in modern agriculture an increasing
regulated world. Annual Review of Entomology 51:45-
66.
Mitchell, J. (2002). Termites are pests of crops, forestry,
rangeland and structures in Southern Africa and their
control. Sociobiology 40: 47-69.
Nigussie Lulie and Nagappan, R. (2012). Evaluation of
Certain Botanical Preparations against African
Bollworm, Helicover pagrmiyera Hubner (Lepidopera:
noctuidae) and Non Target Organisms in Ckickpea,
Cicer arietinum. Journal of bio-fertilizer and Bio-
pesticides 3(5): 130.
Njoroge, G. and Bussmann, R. (2006). Traditional
management of Ear, Nose and Throat diseases in
Central Kenya. Journal of Ethno biology and Ethno
medicine 2: 54.
Prabakar, K. and Jebanesan, A. (2004). Larvicidal efficacy
of some Cucurbitaceous plant leaf extract against
Culex quinquefasicatus. Bioresource Technology
95:113-114.
Rajesh, S. and Vikas, S. (2014). Efficacy of ethanolic
extracts of Brassica nigra as potential antimicrobial
agent against seed microorganisms. International
Journal of Pharmaceutical 3:117-122.
Sahay, N. Prajapati, C. and Singh, P. (2014). Anti-termite
potential of plants selected from the SRISTI database
of Grassroots Innovations. Journal of Biological Pest
Control 7:164-169.
Sakasegawa, M., Hori, K. and Yatagi, M. (2003).
Composition and anti-termite activities of essential oils
and Melaleuca species. Journal of Wood Science
49:181-187.
Sattar, A., Naeem, M. and Ehsan, U. (2014). Efficacy of
Plant extracts against Termites, Microtermes obesi and
Odontotermes lokanandi. Journal of Biodiversity and
Development 1:22.
Tadele Shibru, Habtamu Ashagre and Mulugeta Negeri
(2014). Effect of Some Botanicals against Termites,
Macroterms Spp. (Isoptera: Termitidae) Under
Laboratory Conditions. Ambo University, Ethiopia.
International Journal of Sustainable Agricultural
Research 1(2): 52-57.
Upadhyay, R.K. (2013). Effects of plant latex based anti-
termite formulations on Indian white termite,
Odontotermes obesus (Isoptera: Odontotermitidae) in
sub-tropical high infestation areas. Journal of Animal
Science 3(4): 281-294.