This document discusses various medical applications of enzymes including enzyme therapy for diseases of organs like the liver and kidney, enzyme deficiency diseases, and clinical diagnosis. It also describes the use of enzymes in disease treatment like amylase for digestive disorders and collagenase for skin ulcers. Specific enzymes are discussed in relation to diseases like asparaginase for leukemia and lysozyme as an antibiotic. The roles of enzymes in determining metabolites and diseases are also summarized briefly in 3 sentences or less.

1. MEDICAL APPLICATION OF ENZYMES HARIS P MSC BIOTECHNOLOGY MA COLLEGE ERNAKULAM

4. ENZYMES IN DISEASES Organ Enzymes Diseases Liver ALP,GDH,SGOT,SGPT,Glutamyl Tranferasess,Glutathione S Tranferases, Viral/toxic hepatitis or cirrhosis, myocardial infraction,jaundice, Heart Creatne Kinases,LDH, AST/ALT, Myocardial Infraction , skelital muscle disorders. Other diseases Amylases,Aldolases,TG Lipases,CK,ALP,ACP,Choline Esterases,HMG CO A Reductases,Xanthine Oxidases, GIT disorders, jaundice,bone diseases, rickets,

6. ENZYME DEFICIENCY DISEASES Diseases Enzyme Gout (X linked recessive) PRPP Synthase Lesch Nyhan Syndrom hypoxanthine-guanine- Phosphoribosyl transferase Cystic fibrosis DNase Alcaptonuria Homogentisate oxidase Maple syrup urine disease (various forms Branched-chain ketoacid dehydrogenase Immunoinsufficiency( autosomal Recessive) Adenosinedeaminase Disease Enzyme Tay-Sachs disease hexosaminidase A Krabbe's disease b-galactosidase Niemann-Pick disease sphingomyelinase Farber's disease ceramidase Fabry's disease a-galactosidase Alcaptonuria homogentisate oxidase Gauchers desease b-glucosidase Lactose intolerance Lactase

8. Enzyme inhibitors & Drug Design Drug/Inhibitor Enzyme Disease Mevinolin, Copactin, Monacolk HMG CoA Reductase Hypercholesteremia Penicillins & antibiotics Peptidyl transferase, RNA polumerases,DNA Polymerases etc. Infections after Surgery,Other infectious diseases Allopurine Xanthine Oxidases Gout, Hyperureamia HIV Protease Inhibitors HIV Proteases AIDS Methotrexate Folate reductase Cancer,infections Ethanol Alcohol Dehydrogenase Methanol poisoning,

10. CANCER THERAPY PRODRUG DRUG ENZYME Methotrexate  phe. methotrexate Carboxy peptidase A Etoposide phosphate Etoposide ALP  N bis 2-chloroehyl aminobezoyl glu. Benzoic acid mustard Carboxypeptidase-G2 Mtomycin phosphate mitomycin ALP Aniline mustard glucuronide Aniline mustar Glucuronidase 5-flurrocytosine 5-flurouracinl Cytosine deaminase 2 L  amino acyl methotrexate mmethotrexate aminopeptidase