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Basic Flower Structure
stigma
style
ovary
locule
ovule
carpel
gynoecium
pollen
anther
filament stamen
androecium
petal
corolla
perianthsepal
calyx
receptacle
pedicel
Perianth is complete
Flower is perfect
Flower is monoecious
superior
Flower is hypogynous
Pistil is simple
pollen dehiscence
pollination
pollen tube growth
syngamy
x
vectors: animals, wind
Flower Functions Leading to Sex
FLOWERING PLANT REPRODUCTION
•Flowers are modified leaves,
specialized for reproduction.
•Flower parts undergo meiosis
to produce haploid products.
•Pollen grain
•Ovule (contains egg cell)
Pollen
grains
Ovule
Flower
Meiosis
Mitosis
Mitosis
• The plant life cycle is characterized by the alternation of
generations between a haploid sporophyte and a diploid
gametophyte.
• The sporophyte functions to produce spores, which then
develop into gametophytes.
• The differentiated gametophytes in turn produce either
the male gametes (sperm) or female gametes (egg cells).
• In contrast to lower plant species, in which the
gametophyte is the dominant, free-living generation .
• Gametophytes of angiosperms are smaller and less
complex than the sporophyte and are formed within
specialized organs of the flower.
Pollen
grains
Ovule
Flower
Meiosis
Mitosis
Mitosis
SPOROGENESIS
•Production of microspores and megaspores
•Microspores – anther
•Megaspores - ovules
Pollen grain develops in
chambers within the anther
Embryo sac develops inside ovule
which in turn is enclosed in ovary
POLLEN AND OVULE
DEVELOPMENT
WHERE POLLEN IS PRESENT..?
Anther Cross Section - How many anther sacs are present?
epidermis
vein
mesophyll
pollen grains
MICROSPOROGENESIS
•Each anther has 4 pollen sac – numerous pollen
mother cells.
•Each PMC undergoes meiosis to produce 4
haploid cells – microspores.
•Matures into pollen grain by means of thickening
cell wall.
HaploidHaploid
HaploidHaploid
Pollen grainTube Cell Generative
Cellcytoplasm
nucleus
nucleolus
cytoplasm
nucleus
nucleolus
Pollen Wall
Waterproofing prevents desiccation during pollen transfer. Recognition proteins cause
allergic reactions.
MEGASPOROGENESIS
•Single cell in each ovule differentiate
into a megaspore mother cell.
•It undergoes meiosis to produce 4
haploid megaspores.
•3 – degenerated leaving one functional
megaspore per ovule.
GAMETOGENESIS
•Production of male and female gametes in the microspores and the megaspores
•Micro gametogenesis
•Mega gametogenesis
MICROGAMETOGENESIS
•Production on male gamete of sperm.
•During maturation- microspore nucleus
divides mitotically to produce
•Generative
•Vegetative / tube nuclei
•Pollen generally released in binucleate stage.
•After pollination, the pollen germinates.
•Pollen tube enters the stigma and grows through the style.
•The generative nuclei now undergoes a mitotic division – two male gametes
•The pollen along with the pollen tube- micro gametophyte
•The pollen tube finally enters the ovule through a small pore- micropyle & discharge
2 sperm into embryo sac.
1. Pollen grain deposited by vector on stigma.
2. Tube cell germinates through pollen wall.
4. Generative cell divides to make two sperm cells.
tube
nucleus
tube
nucleus
Synergids burst end of pollen tube. Sperm released into embryo
sac. Syngamy events follow.
3. Generative cell follows pathway of pollen tube.
tube
nucleus
generative
nucleus
pollen
tube
Golgi
and
vesicles
Microsporogenesis and Microgametogenesis
PMC
First
Meiotic
division
Dyad Tetrad
Second
Meitic
division
n n
n n
n n
n
Mitosis
n
n
Generative
nucleus
Microgametogenesis
Mitosis in
generative
nucleus
Microgametophyte
Binucleate
pollen
( Pollination )
Pollen grain
( Microspore)
Microsporogenesis
Anther
MEGAGAMETOGENESIS
• Functional megaspore nucleus divides mitotically – 4 or more nuclei – depend
on the species
• 3 mitotic division – 8 nuclei
• Three nuclei move to one pole – Central egg cell & Two synergid cell either side
• Another 3 move to opposite pole – Antipodal cells
• 2 nuclei remaining in the center- Polar nuclei.
Fuse to form secondary nucleus.
•The megaspore develops into a mature mega gametophyte or embryo sac.
•The development of embryo sac from a megaspore – mega gametogenesis.
EMBRYO SAC
•One egg cell
•Two synergides
•Three antipodal cells
•One diploid secondary nucleus
Embryo Sac in Ovule
Ovule
Embryo
Sac
Antipodals
Central Cell
Egg
Synergids
Integument
Funiculus
Micropyle
Embryo Sac
disintegrat
e
+ sperm = endosperm
+ sperm = zygote
burst pollen tube
release sperm disintegrateMicropyle of Integument
2 Synergid Cells
Egg Cell
Central Cell
3 Antipodal Cells
Integument
Megasporogenesis and Megagametogenesis
2 n
First meiotic division
n
n
second meiotic division n
n
n
n
3 degenerate
Megaspore
Megasporogenesis
Mitosis IMitosis II
Mitosis III
Polar nuclei ( 2 n)
Egg cell
Synergids
Antipodals
Megagametophyte or
Embryosac
Ovule
n
POLLINATION
Landing of active pollen on to a receptive stigma
Pollen grain germinate and pollen tubes enter the stigma
Pollen tube travels through style and reach ovule through Micropyle
Discharge the two sperm nuclei in to the embryo sac
Fertilization :
Fusion of one sperm with egg cell - Fertilization - Zygote
Fusion of another sperm with secondary nucleus - Double Fertilization -
Primary endosperm
Fertilization :
• In flowering plants pollen grains are deposited on the stigmal surface during
anthesis.
• If the pollen and stigma are compatible a pollen tube grows that enters the
intercellular space of the ovary .
• The pollen tube then grows through a specialised tissue of the ovary called the
transmitting tract and emerges at random points on the inner surface of the
carpel.
• It then grows towards the micropyle of the ovule where it delivers two sperm
cells and initiates the double fertilization event within the embryo sac.
Mature Pollen
Grain
POLLINATION AND
FERTILIZATION IN A FLOWER
Tube Cell Nucleus
Sperm Cells
1. pollen grains
land on stigma
Stigma
Style
Ovary
2. pollen tubes grow
down stigma to ovary
Sperm
Egg
3A: Fusion of
sperm + egg
3B: Fusion of 2nd
sperm + two
polar nuclei
3. double
fertilization
Polar nuclei
Ovule
AFTER FERTILIZATION
Development of Fruit and Seeds
From Flower Parts
Seed = Embryo + stored food + seed coat
Fruit = Ovary wall, mechanism for seed dispersal
Triploid
Endosperm
Cell
Ovary
Integument
Diploid
Zygote
Endosperm
Fresh
Fruit
Seed
Coat
Embryo
(new
plant)
Provides nutrition
• Egg becomes Zygote
• Central cell becomes Endosperm
• Ovule becomes Seed
• Integument becomes Seed coat
• Ovary wall becomes Fruit
38
Embryology of pollen and ovule development
Embryology of pollen and ovule development

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Embryology of pollen and ovule development

  • 1.
  • 2. Basic Flower Structure stigma style ovary locule ovule carpel gynoecium pollen anther filament stamen androecium petal corolla perianthsepal calyx receptacle pedicel Perianth is complete Flower is perfect Flower is monoecious superior Flower is hypogynous Pistil is simple
  • 3. pollen dehiscence pollination pollen tube growth syngamy x vectors: animals, wind Flower Functions Leading to Sex
  • 4. FLOWERING PLANT REPRODUCTION •Flowers are modified leaves, specialized for reproduction. •Flower parts undergo meiosis to produce haploid products. •Pollen grain •Ovule (contains egg cell) Pollen grains Ovule Flower Meiosis Mitosis Mitosis
  • 5. • The plant life cycle is characterized by the alternation of generations between a haploid sporophyte and a diploid gametophyte. • The sporophyte functions to produce spores, which then develop into gametophytes. • The differentiated gametophytes in turn produce either the male gametes (sperm) or female gametes (egg cells). • In contrast to lower plant species, in which the gametophyte is the dominant, free-living generation . • Gametophytes of angiosperms are smaller and less complex than the sporophyte and are formed within specialized organs of the flower. Pollen grains Ovule Flower Meiosis Mitosis Mitosis
  • 6. SPOROGENESIS •Production of microspores and megaspores •Microspores – anther •Megaspores - ovules
  • 7.
  • 8. Pollen grain develops in chambers within the anther Embryo sac develops inside ovule which in turn is enclosed in ovary
  • 10. WHERE POLLEN IS PRESENT..?
  • 11. Anther Cross Section - How many anther sacs are present? epidermis vein mesophyll pollen grains
  • 12. MICROSPOROGENESIS •Each anther has 4 pollen sac – numerous pollen mother cells. •Each PMC undergoes meiosis to produce 4 haploid cells – microspores. •Matures into pollen grain by means of thickening cell wall. HaploidHaploid HaploidHaploid
  • 13. Pollen grainTube Cell Generative Cellcytoplasm nucleus nucleolus cytoplasm nucleus nucleolus Pollen Wall Waterproofing prevents desiccation during pollen transfer. Recognition proteins cause allergic reactions.
  • 14. MEGASPOROGENESIS •Single cell in each ovule differentiate into a megaspore mother cell. •It undergoes meiosis to produce 4 haploid megaspores. •3 – degenerated leaving one functional megaspore per ovule.
  • 15. GAMETOGENESIS •Production of male and female gametes in the microspores and the megaspores •Micro gametogenesis •Mega gametogenesis
  • 16. MICROGAMETOGENESIS •Production on male gamete of sperm. •During maturation- microspore nucleus divides mitotically to produce •Generative •Vegetative / tube nuclei •Pollen generally released in binucleate stage.
  • 17. •After pollination, the pollen germinates. •Pollen tube enters the stigma and grows through the style. •The generative nuclei now undergoes a mitotic division – two male gametes •The pollen along with the pollen tube- micro gametophyte •The pollen tube finally enters the ovule through a small pore- micropyle & discharge 2 sperm into embryo sac.
  • 18. 1. Pollen grain deposited by vector on stigma. 2. Tube cell germinates through pollen wall. 4. Generative cell divides to make two sperm cells. tube nucleus tube nucleus Synergids burst end of pollen tube. Sperm released into embryo sac. Syngamy events follow. 3. Generative cell follows pathway of pollen tube. tube nucleus generative nucleus pollen tube Golgi and vesicles
  • 19. Microsporogenesis and Microgametogenesis PMC First Meiotic division Dyad Tetrad Second Meitic division n n n n n n n Mitosis n n Generative nucleus Microgametogenesis Mitosis in generative nucleus Microgametophyte Binucleate pollen ( Pollination ) Pollen grain ( Microspore) Microsporogenesis Anther
  • 20.
  • 21. MEGAGAMETOGENESIS • Functional megaspore nucleus divides mitotically – 4 or more nuclei – depend on the species • 3 mitotic division – 8 nuclei • Three nuclei move to one pole – Central egg cell & Two synergid cell either side • Another 3 move to opposite pole – Antipodal cells • 2 nuclei remaining in the center- Polar nuclei.
  • 22. Fuse to form secondary nucleus. •The megaspore develops into a mature mega gametophyte or embryo sac. •The development of embryo sac from a megaspore – mega gametogenesis.
  • 23. EMBRYO SAC •One egg cell •Two synergides •Three antipodal cells •One diploid secondary nucleus
  • 24. Embryo Sac in Ovule Ovule Embryo Sac Antipodals Central Cell Egg Synergids Integument Funiculus Micropyle
  • 25. Embryo Sac disintegrat e + sperm = endosperm + sperm = zygote burst pollen tube release sperm disintegrateMicropyle of Integument 2 Synergid Cells Egg Cell Central Cell 3 Antipodal Cells Integument
  • 26. Megasporogenesis and Megagametogenesis 2 n First meiotic division n n second meiotic division n n n n 3 degenerate Megaspore Megasporogenesis Mitosis IMitosis II Mitosis III Polar nuclei ( 2 n) Egg cell Synergids Antipodals Megagametophyte or Embryosac Ovule n
  • 27.
  • 28.
  • 29. POLLINATION Landing of active pollen on to a receptive stigma Pollen grain germinate and pollen tubes enter the stigma Pollen tube travels through style and reach ovule through Micropyle Discharge the two sperm nuclei in to the embryo sac
  • 30. Fertilization : Fusion of one sperm with egg cell - Fertilization - Zygote Fusion of another sperm with secondary nucleus - Double Fertilization - Primary endosperm
  • 31. Fertilization : • In flowering plants pollen grains are deposited on the stigmal surface during anthesis. • If the pollen and stigma are compatible a pollen tube grows that enters the intercellular space of the ovary . • The pollen tube then grows through a specialised tissue of the ovary called the transmitting tract and emerges at random points on the inner surface of the carpel. • It then grows towards the micropyle of the ovule where it delivers two sperm cells and initiates the double fertilization event within the embryo sac.
  • 32. Mature Pollen Grain POLLINATION AND FERTILIZATION IN A FLOWER Tube Cell Nucleus Sperm Cells 1. pollen grains land on stigma Stigma Style Ovary 2. pollen tubes grow down stigma to ovary Sperm Egg 3A: Fusion of sperm + egg 3B: Fusion of 2nd sperm + two polar nuclei 3. double fertilization Polar nuclei Ovule
  • 33.
  • 34. AFTER FERTILIZATION Development of Fruit and Seeds From Flower Parts
  • 35. Seed = Embryo + stored food + seed coat Fruit = Ovary wall, mechanism for seed dispersal Triploid Endosperm Cell Ovary Integument Diploid Zygote Endosperm Fresh Fruit Seed Coat Embryo (new plant) Provides nutrition
  • 36. • Egg becomes Zygote • Central cell becomes Endosperm • Ovule becomes Seed • Integument becomes Seed coat • Ovary wall becomes Fruit
  • 37.
  • 38. 38