Dynamic light scattering measures the fluctuation changes in the intensity of scattered light to determine particle size and properties. It works by measuring the rate at which the intensity of scattered light fluctuates due to Brownian motion of particles. Larger particles diffuse more slowly than smaller particles, so intensity fluctuations are slower for large particles. The correlation function contains information about particle diffusion, with steeper curves indicating more monodisperse samples and more extended decay indicating greater polydispersity. Dynamic light scattering can determine particle size distribution, hydrodynamic radius, and diffusion coefficient.
Dynamic light scattering (DLS) is a technique in physics that can be used to determine the size distribution profile of small particles in suspension or polymers in solution.
Other names are
Photon correlation spectroscopy
Quasi-elastic light scattering.
Dynamic light scattering (DLS) is a technique in physics that can be used to determine the size distribution profile of nanoparticles in suspension or in polymers
Dynamic light scattering (DLS) or Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.
In This slide the working principle and the function of DLS is Explained in brief and precise way.
Dynamic light scattering (DLS) is a technique in physics that can be used to determine the size distribution profile of small particles in suspension or polymers in solution.
Other names are
Photon correlation spectroscopy
Quasi-elastic light scattering.
Dynamic light scattering (DLS) is a technique in physics that can be used to determine the size distribution profile of nanoparticles in suspension or in polymers
Dynamic light scattering (DLS) or Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.
In This slide the working principle and the function of DLS is Explained in brief and precise way.
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions.
Introduction
Nanoparticle characterization techniques
Electron Microscope
Scanning electron microscope
Transmission electron Microscope
X-ray powder diffraction
Nuclear Magnetic Resonance
Microwave Assisted Synthesis of Metallic NanostructuresJoyce Joseph
It gives you the brief idea about synthesizing metallic nanoparticles, an introduction to this concept in simple terms.
Hoping to give you guys more detail on it soon
Different types of methods can be used for the preparation of Magnetic Nanoparticles, their advantages and disadvantages and applications of the materials in various fields are given in the presentation
Nanoparticles are particles between 1 and 100 nanometres in size with a surrounding interfacial layer. The interfacial layer is an integral part of nanoscale matter, fundamentally affecting all of its properties. The interfacial layer typically consists of ions, inorganic and organic molecules.
Dynamic light scattering, also known as photon correlation spectroscopy or quasi-elastic light scattering, is a technique that primarily measures the Brownian motion of macromolecules in solution that arises due to bombardment from solvent molecules, and relates this motion to the size
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions.
Introduction
Nanoparticle characterization techniques
Electron Microscope
Scanning electron microscope
Transmission electron Microscope
X-ray powder diffraction
Nuclear Magnetic Resonance
Microwave Assisted Synthesis of Metallic NanostructuresJoyce Joseph
It gives you the brief idea about synthesizing metallic nanoparticles, an introduction to this concept in simple terms.
Hoping to give you guys more detail on it soon
Different types of methods can be used for the preparation of Magnetic Nanoparticles, their advantages and disadvantages and applications of the materials in various fields are given in the presentation
Nanoparticles are particles between 1 and 100 nanometres in size with a surrounding interfacial layer. The interfacial layer is an integral part of nanoscale matter, fundamentally affecting all of its properties. The interfacial layer typically consists of ions, inorganic and organic molecules.
Dynamic light scattering, also known as photon correlation spectroscopy or quasi-elastic light scattering, is a technique that primarily measures the Brownian motion of macromolecules in solution that arises due to bombardment from solvent molecules, and relates this motion to the size
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.
Particle Size Analysis by Laser Diffraction Method. AshviniTanpure
For Determination of Particle Size various method are used. here I mentioned the Laser Light scattering for determining the Particle Size. Mainly two type of laser scattering are used,
1. Static laser light scattering.
2. Dynamic laser light scattering.
detail about there principle we see in the slide .
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If in any point you didn't understood ,you can contact with me.
hope it useful to you.
Thank You.
Light Scattering Phenomenon:
The blue color of the sky and the red color of the sun at sunset result from scattering of light of small dust particles, H2O molecules and other gases in the atmosphere.
The efficiency with which light is scattered depends on its wavelength(λ).
The sky is blue because violet and blue light are scattered to a greater extent than other longer wavelengths.
A clear cloudless day-time sky is blue because molecules in the air scatter blue light from the sun more than they scatter red light.
When we look towards the sun at sunset, we see red and orange colours because the blue light has been scattered out and away from the line of sight.
Scattered radiation:
• Radiate scattering- second major spectral method of analysis.
• In this technique some radiation that passes through a sample strikes particles of the analyte and is scattered in a different direction.
• A detector is used to measure either the intensity of the scattered radiation or the decreased intensity of the incident radiation
• Depending on the scattering mechanism, the method can be employed for either qualitative or quantitative analysis.
For chemical analysis three forms of radiative scattering are important – viz.
Tyndall,
Raman, and
Rayleigh scattering.
Tyndall Scattering occurs when the dimensions of the particles that are causing the scattering are larger than the wavelength of the scattered radiation.
It is caused by reflection of the incident radiation from the surfaces of the particles,
reflection from the interior walls of the particles, and refraction and diffraction of the radiation as it passes through the particles.
Scattering of light
- by particles in a colloid or suspension.
The longer-wavelength light is more transmitted while the shorter- wavelength light is more reflected via scattering
Nephelometry & Turbidimetry:
When electromagnetic radiation (light) strikes a particle in solution, some of the light will be absorbed by the particle, some will be transmitted through the solution and some of the light will be scattered or reflected .
The amount of light scattered is proportional to the concentration of insoluble particle.
In Nephelometry, the intensity of the scattered light is measured.
In Turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. Light scattering is the physical phenomenon resulting from the interaction of light with a particles in solution
Turbidimetry is involved with measuring the amount of transmitted light (and calculating the absorbed light) by particles in suspension to determine the concentration of the substance in question.
Amount of absorbed light, and therefore, concentration is dependent on ;
1) number of particles, and
2) size of particles.
• Measurements are made using light spectrophotometers
Factors affecting on scattering of light:
Concentration of particles
Particle size
Wavelength
Distance of
The distribution and_annihilation_of_dark_matter_around_black_holesSérgio Sacani
Uma nova simulação computacional feita pela NASA mostra que as partículas da matéria escura colidindo na extrema gravidade de um buraco negro pode produzir uma luz de raios-gamma forte e potencialmente observável. Detectando essa emissão forneceria aos astrônomos com uma nova ferramenta para entender tanto os buracos negros como a natureza da matéria escura, uma elusiva substância responsável pela maior parte da massa do universo que nem reflete, absorve ou emite luz.
Identification of the Memory Process in the Irregularly Sampled Discrete Time...idescitation
In the present work, we have considered the daily
signal of Solar Radio flux of 2800 Hz cited by National
Geographic Data Center, USA during the period from 29 th
October, 1972 to 28th February, 2013. We have applied Savitzky-
Golay nonlinear phase filter on the present discrete signal to
denoise it and after denoising Finite Variance Scaling Method
has been applied to investigate memory pattern in this discrete
time variant signal. Our result indicates that the present signal
of solar radio flux is of short memory which may in turn
suggest the multi-periodic and/or pseudo-periodic behaviour
of the present signal.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
ISI 2024: Application Form (Extended), Exam Date (Out), EligibilitySciAstra
The Indian Statistical Institute (ISI) has extended its application deadline for 2024 admissions to April 2. Known for its excellence in statistics and related fields, ISI offers a range of programs from Bachelor's to Junior Research Fellowships. The admission test is scheduled for May 12, 2024. Eligibility varies by program, generally requiring a background in Mathematics and English for undergraduate courses and specific degrees for postgraduate and research positions. Application fees are ₹1500 for male general category applicants and ₹1000 for females. Applications are open to Indian and OCI candidates.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
2. 2
Static Light Scattering
Measures Total Intensity of Scattered Light
(Mass(M), Size (rg), Second Virial Coefficient (A2)
Dynamic Light Scattering
Measures Fluctuation Changes on The Intensity of the
Scattered light
(Diffusion Constant (DT), Size, Rh, Polydispersity Index)
3. 3
Dynamic Light Scattering
Particle size can be determined by measuring the random change in
intensity of light scattered from suspension.
It measure and interpolate the light scattering up to microsecond.
So it measure real time intensity, thus measuring the dynamic
properties.
Size distribution, Hydrodynamic radius, Diffusion coefficient
4. 4
For measuring Hydrodynamic Size of nanoparticle, protein and biomaterial
One can also study stability of nanoparticles as function of time
Good for detecting the aggregation of the particles
Required small volume of sample
Complete recovery of sample after measurement
Sample preparation is not required for the measurement
Application of DLS
6. 6
In DLS, the speed at which the particles are diffusing due to Brownian motion is
measured. This is done by measuring the rate at which the intensity of the scattered light
fluctuates when detected using a suitable optical arrangement.
7. 7
Brownian motion is the fundamental of this instrument
Brownian motion of the particle is random motion due to the
bombardment by the solvent molecule surround them.
Brownian motion of the particles are related to size.
It describes the way in which very small particles move in fluid
suspension
It is related to the Viscosity and Temperature.
Brownian motion
8. 8
Obtained optical signal shows random change due to random change in the
position of the particle.
The “ noise “ is actually the particle motion and will be used to measure the particle
size.
9. 9
Particles with a large physical dimension (radius) diffuse more slowly
through a solvent, while small particles diffuse more quickly. Intensity
fluctuations seen through time are therefore slower for large particles.
10. 10
A correlation function is statistical correlation between random variables at two
different points in space or time, usually as a function of the spatial or temporal
distance between the points.
Within the correlation curve all of the information regarding the diffusion of
particles within the sample being measured.
11. 11
A correlator is basically a signal comparator. . It is designed to measure the degree
of similarity between two signals, or one signal with itself at varying time
intervals. If the intensity at time t is compared with the intensity at time t+δt, there
will be a strong correlation between two signal.
Correlation of a signal arriving from random source will decrease with time.
If the particle will large the signal will changes slowly and correlation will sustain
for long time.
How does a Correlator Work
14. 14
Typical Correlation Curves
The steeper the curve the more mono disperse the sample is.
More extended the decay becomes the greater the polydispesity.
15. The Correlation Function for monodisperse particle
15
G() = A [ 1 + B exp (-2)]
A = the baseline of the correlation function
B = intercept of the correlation function.
= Dq2
D = translational diffusion coefficient, q = scattering vector
q = (4 n / o) sin (/2)
n = refractive index of dispersant
o = wavelength of the laser
= scattering angle.
20. Polydispersity Index
• 0 to 0.05 - Monodisperse
• 0.05 to 0.08 - Nearly Monodisperse
• 0.08 to 0.7 - Mid Range Polydispersity
• Greater than 0.7 – Very Polydisperse; Probably not
suited for DLS Measurements
20
In dynamic light scattering instrumentation, the correlation summations are performed using an integrated digital correlator. Examples of correlation curves measured for two sub-micron particles are given in Figure 3. For the smaller and hence faster diffusing protein, the measured correlation curve has decayed to baseline within 100 μs, while the larger and slower diffusing silicon dioxide particle requires nearly 1000 μs before correlation in the signal is completely lost.
In dynamic light scattering, all of the information regarding the motion or diffusion of the particles in the solution is embodied within the measured correlation curve. For a large number of monodisperse particles in Brownian motion, the correlation function (given the symbol [G]) is an exponential decaying function of the correlator time delay :
In the light scattering area, the term polydispersity is derived from the polydispersity index, a parameter calculated from a Cumulants analysis of the DLS measured intensity autocorrelation function. In the Cumulants analysis, a single particle size is assumed and a single exponential fit is applied to the autocorrelation function. The autocorrelation function, along with the exponential fitting expression, is shown below, where I is the scattering intensity, t is the initial time, τ is the delay time, A is the amplitude or intercept of the correlation function, B is the baseline, D is the diffusion coefficient, q is the scattering vector, λo is the vacuum laser wavelength, ñ is the medium refractive index, θ is the scattering angle, k is the Boltzmann constant, T is the absolute temperature, η is the viscosity of the medium, and RH is the hydrodynamic radius.
In the Cumulants approach, the exponential fitting expression is expanded to account for polydispersity or peak broadening effects
The expression is then linearized and the data fit to the form shown below, where the D subscript notation is used to indicate diameter. The 1st Cumulant or moment (a1) is used to calculate the intensity weighted Z average mean size and the 2nd moment (a2) is used to calculate a parameter defined as the polydispersity index (PdI).
Note from the figure below that the 1st moment is proportional to the initial slope of the linear form of the correlogram and the
2nd moment is related to the inflection point at which log G deviates from linearity.
While the Cumulant algorithm and the Z average are useful for describing general solution characteristics, for multimodal solutions consisting of multiple particle size groups, the Z average can be misleading. For multimodal solutions, it is more appropriate to fit the correlation curve to a multiple exponential form, using common algorithms such as CONTIN or Non Negative Lease Squares (NNLS).