DNA fingerprinting of plant material from farmers' fields has provided new insights. Traditional surveys relying on farmer self-reporting often overestimate adoption of improved varieties. Eight new studies using DNA fingerprinting found adoption rates were typically lower than reported. False positive and negative rates varied by crop and country. On average, only 40-60% of samples were correctly identified as improved or local varieties. Low genetic purity was also common, with many samples consisting of mixed varieties. These findings have implications for understanding impacts of new varieties and for seed systems.
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
Molecular Marker and It's ApplicationsSuresh Antre
Molecular (DNA) markers are segments of DNA that can be detected through specific laboratory techniques. With the advent of marker-assisted selection (MAS), a new breeding tool is now available to make more accurate and useful selections in breeding populations.
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
B4FA 2012 Tanzania: Genetics, plant breeding and agriculture - Tina Barsbyb4fa
Presentation at the November 2012 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Arusha, Tanzania.
Please see www.b4fa.org for more information
Molecular Marker and It's ApplicationsSuresh Antre
Molecular (DNA) markers are segments of DNA that can be detected through specific laboratory techniques. With the advent of marker-assisted selection (MAS), a new breeding tool is now available to make more accurate and useful selections in breeding populations.
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
B4FA 2012 Tanzania: Genetics, plant breeding and agriculture - Tina Barsbyb4fa
Presentation at the November 2012 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Arusha, Tanzania.
Please see www.b4fa.org for more information
Presentation given by Bioversity International's Carlo Fadda at the Mutual Implementation of the Nagoya Protocol and Plant Treaty Workshop, Addis Ababa, 16-20 November 2015
At the Oxford Biodiversity Institute Symposium on 2-3 October 2013, Bioversity International Programme Leader Ehsan Dulloo presented on the importance of genetic diversity for building resilience for crops. Learn more: http://www.bioversityinternational.org/research-portfolio/conservation-of-crop-diversity/
Scaling up Ethiopia’s ‘Seeds for Needs’ approach of using agricultural biodiv...Bioversity International
Bioversity International scientist Carlo Fadda presents to the World Bank on the results we have had so far working with partners in Ethiopia to tap into the genetic diversity of the country and the knowledge of farmers, to help them adapt better to climate change.
Find out more about Seeds for Needs: www.bioversityinternational.org/research-portfolio/adaptation-to-climate-change/seeds-for-needs/
Andrew Kiggundu
Abstract
Late blight (LB) of potato, caused by Phytophthora infestans, is one of the most devastating diseases of potato (Solanum tuberosum L.) in Africa and in many parts of the world. Conventional cross-breeding to develop resistance has met with challenges of length of breeding time and durability of resistance in the hybrids developed. Three resistance genes (RB, Rpi-blb2, and Rpi-vnt1.1) previously cloned from two wild potatoes, S. bulbocastanum and S. venturii, and the availability of a potato transformation system allowed us to introduce all three genes into cultivated varieties in order to develop durable resistance. The genes were introduced into two cultivated potato varieties ‘Desiree’ and ‘Victoria’. The genetically modified (GM) potatoes generated were field-evaluated in Uganda at the Kachwekano Zonal Agricultural Research and Development Institute in Kabale. Twelve transgenic events of the variety ‘Desiree’ and one of the variety ‘Victoria’ were tested in three replications, each consisting of randomised complete block design of plots of 15 plants each. The results indicate that all 13 transgenic events are completely resistant to LB disease. These GM potatoes, if incorporated into the seed potato system for uptake by farmers in Africa and elsewhere, offer a great opportunity for durable, cost-effective, and environmentally friendly solution to the management of LB disease in potato. Consequently, farmers could discontinue their current practice of spraying toxic chemicals to control the disease. The next steps for further development of these lines into commercial varieties for Africa and the associated deregulation challenges are discussed
1. High and stable tuber yield
2. Tuber characteristics that facilitate harvesting and are valued by consumers (e.g. shape, size, storability, dry matter content and food quality)
3. Resistance to biotic stresses (e.g. viruses, anthracnose, nematodes)
4. Tolerance to abiotic stresses (e.g. low soil nutrients, drought)
5. Suitability to major cropping systems (e.g. plant architecture, and maturity period).
6. Nutritional value and metabolites
Partnering on crop wild relative research at three scales: commonalities for ...CWRofUS
The potential for crop wild relatives (CWR) to contribute to crop improvement is growing due to improvements in information on species and their diversity, advancements in breeding tools, and the growing need for exotic genetic diversity to address compounding agronomic challenges. As wild plants, CWR are subject to a myriad of human caused threats to natural ecosystems, and their representation ex situ is often far from comprehensive. Ex situ conservation of many of these wild plants is also technically challenging, particularly in an environment of insufficient resources. Enhancing conservation, availability, and access to CWR requires a spectrum of action spanning basic and applied research on wild species to inform on-the-ground collecting, ex situ maintenance, and germplasm utilization. The development of effective information channels and productive partnerships between diverse organizations are essential to the success of these actions. Here we report on a spectrum of CWR activities involving broad partnerships, at three levels: a) the collaborative compilation and distribution on over 5 million occurrence data records on the CWR of major food crops, b) the analysis of conservation concerns and genetic resources potential of the CWR of potato, sweetpotato, and pigeonpea, and c) ongoing efforts to map the diversity and conservation concerns for CWR in the USA. Although differing in scales and depth of collaborations, the success of these initiatives are largely due to commonalities in research orientation, e.g., inclusiveness, offering clear incentives for involvement, and service providing to the crop science community.
Partnering on CWR research at three scales: commonalities for successCWR Project
The potential for crop wild relatives (CWR) to contribute to crop improvement is growing due to improvements in information on species and their diversity, advancements in breeding tools, and the growing need for exotic genetic diversity to address compounding agronomic challenges. As wild plants, CWR are subject to a myriad of human caused threats to natural ecosystems, and their representation ex situ is often far from comprehensive. Ex situ conservation of many of these wild plants is also technically challenging, particularly in an environment of insufficient resources. Enhancing conservation, availability, and access to CWR requires a spectrum of action spanning basic and applied research on wild species to inform on-the-ground collecting, ex situ maintenance, and germplasm utilization. The development of effective information channels and productive partnerships between diverse organizations are essential to the success of these actions. Here we report on a spectrum of CWR activities involving broad partnerships, at three levels: a) the collaborative compilation and distribution on over 5 million occurrence data records on the CWR of major food crops, b) the analysis of conservation concerns and genetic resources potential of the CWR of potato, sweetpotato, and pigeonpea, and c) ongoing efforts to map the diversity and conservation concerns for CWR in the USA. Although differing in scales and depth of collaborations, the success of these initiatives are largely due to commonalities in research orientation, e.g., inclusiveness, offering clear incentives for involvement, and service providing to the crop science community.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
DNA fingerprinting of plant material from farmers fields:What have we learned? James Stevenson
1. DNA fingerprinting of plant material
from farmers fields: What have we learned?
James Stevenson (ISPC Secretariat)
work with Talip Kilic, John Ilukor, Mywish Maredia, and many others...
2. “As has often been remarked, probably no two individuals are
identically the same… when the eye is well practiced, the
shepherd knows each sheep, and man can distinguish a
fellowman out of millions and millions of other men”
Charles Darwin, 1868,
The variation in animals and plants under domestication, vol. 1, p. 361
3. “As has often been remarked, probably no two individuals are
identically the same… when the eye is well practiced, the
shepherd knows each sheep, and man can distinguish a
fellowman out of millions and millions of other men”
Charles Darwin, 1868,
The variation in animals and plants under domestication, vol. 1, p. 361
• Darwin may have been considerably less optimistic about our
ability to distinguish variation within a population had he
studied maize in farmers’ fields in sub-Saharan Africa…
4. Content
• Adoption of improved varieties: How do we normally get these
data?
• DNA fingerprinting: Overview of field implications of methodology
• Methods and results from 8 new empirical field-based studies
• Implications for CGIAR
• For study of adoption and impacts of new varieties
• For genebanks?
5. • Adoption of improved varieties: How do we normally get these
data?
• DNA fingerprinting: Overview of field implications of methodology
• Methods and results from 8 new empirical field-based studies
• Implications for CGIAR
• For study of adoption and impacts of new varieties
• For genebanks?
Content
6. Varietal identification used to be easy
Early period of the Green Revolution underwritten by a huge turnover
of genetic material in farmers’ fields.
Semi-dwarf varieties spread rapidly through the irrigated wheat and
rice production systems of a number of Asian countries
Adoption in this case represented a very significant shift: the
improved varieties were immediately noticeable to the naked eye -
they looked different
8. Varietal identification used to be easy
Reliable data on adoption of improved varieties has long been
recognized as the cornerstone of any assessment of the impact of
investments in plant breeding (Dalrymple, 1978; Walker and Crissman,
1996; Evenson and Gollin, 2003; CGIAR Science Council, 2008; Walker
and Alwang, 2015)
In the decades since the Green Revolution, the focus of breeding has
diversified significantly in two dimensions:
• diversification across crops
• diversification of the targets for breeding
Poses deep challenge to the process of understanding the adoption of
new varieties in farmers’ fields
9. Current practice in adoption studies
Methodology has traditionally fallen into one of two categories:
1) “Expert opinion” elicitation in focus groups
+ Bulk of the literature has relied on this method
+ Only feasible way of getting large coverage across crops and
countries where varieties have been released (e.g. SPIA is heavily
invested in this method):
DIIVA (2010 – 2012) 115 crop-country combinations in Africa
SIAC (2013 – 2017) 127 crop-country combinations in S, SE, E Asia
- Unable to rigorously link these estimates to development
outcomes
- Obvious concerns about accuracy and possible bias
10. Current practice in adoption studies
2) Household surveys with farmers self-reporting their varieties
+ Potential to sample in a representative manner
+ Combination of adoption status with other variables in survey
allows for impact evaluation
- Unclear to what extent farmers are able to correctly identify their
own varieties
- Very poor track record of setting up surveys to be:
• statistically representative at policy-relevant scale
• revisited in a panel
• provide public goods beyond “the project”
12. Content
• Adoption of improved varieties: How do we normally get
these data?
• DNA fingerprinting: Overview of field implications of
methodology
• Methods and results from 8 new empirical field-based
studies
• Implications for CGIAR
• For study of adoption and impacts of new varieties
• For genebanks?
13. DNA fingerprinting - steps
1: Sample of
plant material
taken: leaf or
grain
2: Each sample
placed in own
kit with
desiccant (for
leaf) and unique
identifier (bar
code or ID
number)
3: Laboratory
in-country
extracts DNA
(if using grain,
each sample is
first dried and
then ground)
4: DNA from
each sample
placed in 96-
well plates and
shipped for
genotyping
5: Each sample
is compared at
multiple alleles
in the genome
against a
reference
library of
varieties
14. Some (tough) lessons learned
• All this is new to survey enumeration teams
• Threats to samples (to mould; weevil attack) need
vigilance and clear protocol
• Data “cold chain” on the samples essential for
linking to other data (e.g. in a CAPI questionnaire)
• Training… field practice…. (revise field protocol if
needed)…. training… more practice… fieldwork starts
• Having sufficiently high density of genotyping assay
is essential: need to be smart consumers of
commercial lab services
15. Content
• Adoption of improved varieties: How do we normally get
these data?
• DNA fingerprinting: Overview of field implications of
methodology
• Methods and results from 8 new empirical field-based
studies
• Implications for CGIAR
• For study of adoption and impacts of new varieties
• For genebanks?
16. Survey-based methods
Crop Sample Sample
drawn from
DNA
from
A: Expert
opinion
B: Ask is this
improved?
C: Ask for
name
D:
Phenotypic
protocol
Maize,
Uganda
550 2 districts;
random
Grain Yes Yes Yes Yes
Sweet potato,
Ethiopia
231 Wolayita;
snowball
Leaf Yes Yes Yes
Cassava,
Malawi
1,200 National;
random
Leaf Yes Yes Yes
Beans, Zambia 855 2 provinces;
random
Seed Yes Yes
Cassava,
Nigeria
2,500 National;
random
Leaf Yes
Cassava,
Vietnam
1570 National;
random
Leaf Yes Yes Yes
Rice,
Indonesia
798 Lampung
province;
random
Seed Yes Yes
Cassava,
Ghana
914 3 regions;
random
Leaf Yes Yes Yes
18. False positives, false negatives
• Aggregate breakdown of adopters vs non-adopters of any
improved variety is only relevant for limited number of
questions
• Typically want to link adoption status (“treatment”) to some
dependent variable of interest (e.g. productivity; HH income)
conditioned by a bunch of covariates
Farmer states IV Farmers states local
/ traditional
Fingerprint = IV Correct positive False negative
Fingerprint = not IV False positive Correct negative
19. False negatives, false positives
% false negatives % false positives
Maize, Uganda 43 0
Sweet potato, Ethiopia 20 30
Cassava, Malawi 21 0.2
Cassava, Nigeria 28 13
• No consistent deflation or inflation possible across all cases:
context-specificity
• If there are more farmers growing improved varieties that
don’t realize it, why aren’t yields higher?
• Noise? Or bias?
20. Genotype Farmer-stated
Maize in Uganda: SPIA / LSMS-
ISA / UBoS / Diversity Arrays
• Data from 540 HHs in 45
enumeration areas
• Enumerators from UBoS
trained for 1 month
• CAPI-based survey + grain-
based highly-quantitative
DArTSeq genotyping
• 2% of farmers were correct
about the variety they were
growing
22. Share of primary genetic
constituent
Green = >70%
Amber = 60 – 70%
Red = <60%
23.
24. Competing theories
• Deliberate mixing by farmers
Farmers skillfully use seeds of different varieties together in the
same plot
• Counterfeiting / deception
Growing literature on input quality as a drag on agricultural
development (e.g. Bold et al, 2015)
• General chaos / informality of seed system
Things start out fine from seed companies but errors accumulate in
different stages in seed supply chain
2016/17 extension: Testing these theories for maize in Uganda
Second visit to the farms in the sample; mystery shopping from agro-
dealers; and testing samples from seed companies / NARS
25. Content
• Adoption of improved varieties: How do we normally get
these data?
• DNA fingerprinting: Overview of field implications of
methodology
• Methods and results from 8 new empirical field-based
studies
• Implications for CGIAR
• For study of adoption and impacts of new varieties
• For genebanks?