This document discusses the mass selection method of plant breeding. Mass selection involves selecting individual plants based on phenotype from a mixed population, bulking their seeds to grow the next generation. It is one of the oldest methods of crop improvement and can be used for both self- and cross-pollinated species. The goals of mass selection are to increase the frequency of superior genotypes, purify mixed populations, and develop new cultivars by improving average population performance through repeated selection and seed bulking over multiple generations.
Plant breeding methods of vegetatively propagated crops Roksana Aftab Ruhi
Vegetatively propagated crops are bred by intentionally crossing of closely or distantly related individual to produce new crop varieties or lines with desirable traits. Breeding of vegetative crops have successfully improved quality, yield, tolerance of crops to environmental pressure. Breeding helps in producing crops that are resistant to viruses, fungi and bacteria and helps in longer storage period for the harvested crop.
Mutagenesis is the process by which the genetic information
of an organism is changed in a stable manner.
The term ‘mutation breeding’ has become popular as it
draws attention to deliberate efforts of breeders and
the specific techniques they have used in creating and
harnessing desired variation in developing elite breeding
lines and cultivated varieties.
Clone is the progeny of a single plant, produced by asexual reproduction
Clonal selection is the selection of the most desirable members of a clone for continued vegetative propagation rather than for sexual reproduction.
The members of a clone keep up genetic constancy.
So by clonal selection and continued vegetative propagation, the desirable qualities of plants can be maintained for long.
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
Centres of diversity – types of biodiversity – Centres of origin – Law of homologous series – centers of origin – types of centres of diversity – gene sanctuaries – genetic erosion – main reasons of genetic erosion – extinction – introgression – gene banks – types of gene banks
Plant breeding methods of vegetatively propagated crops Roksana Aftab Ruhi
Vegetatively propagated crops are bred by intentionally crossing of closely or distantly related individual to produce new crop varieties or lines with desirable traits. Breeding of vegetative crops have successfully improved quality, yield, tolerance of crops to environmental pressure. Breeding helps in producing crops that are resistant to viruses, fungi and bacteria and helps in longer storage period for the harvested crop.
Mutagenesis is the process by which the genetic information
of an organism is changed in a stable manner.
The term ‘mutation breeding’ has become popular as it
draws attention to deliberate efforts of breeders and
the specific techniques they have used in creating and
harnessing desired variation in developing elite breeding
lines and cultivated varieties.
Clone is the progeny of a single plant, produced by asexual reproduction
Clonal selection is the selection of the most desirable members of a clone for continued vegetative propagation rather than for sexual reproduction.
The members of a clone keep up genetic constancy.
So by clonal selection and continued vegetative propagation, the desirable qualities of plants can be maintained for long.
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
Centres of diversity – types of biodiversity – Centres of origin – Law of homologous series – centers of origin – types of centres of diversity – gene sanctuaries – genetic erosion – main reasons of genetic erosion – extinction – introgression – gene banks – types of gene banks
SELECTION METHODS IN SELF-POLLINATED CROPS viz., mass selection, pureline sel...AMIT RANA Ph. D Scholar
MASS SELECTION
Mass selection is a method of breeding in which individual plants are selected on the basis of phenotype from a mixed population , their seeds are bulked and used to grow the next generation.
Selection cycle may be repeated one or more times to increase the frequency of favorable alleles - phenotypic recurrent selection.
PURELINE SELECTION
A pureline is the progeny of a single homozygous plant of a self-pollinated species. All the plants in a pureline have the same genotype and the phenotypic variation within a pureline is due to the environment alone and has no genetic basis. However, variation within a pureline is not heritable. Hence selection in a pureline is not effective. Johannsen (1903,1926), a Danish biologist, developed the concept of pureline theory working with Princess variety of French bean (Phaseolus vulgaris), which showed variation for seed size. From a commercial seed lot he selected seeds of different sizes and grew them separately. The progenies differed in seed size. Progenies from larger seeds produced larger seeds than those obtained from smaller seeds. This clearly showed that the variation in seed size in the commercial seed lot of princess variety had a genetic base. As a result selection for seed size was effective.
Introduction
PEDIGREE SELECTION
Pedigree selection is a widely used method of breeding self-pollinated species.
A key difference between pedigree selection and mass selection or pure-line selection is that hybridization is used to generate variability (for the base population), unlike the other methods in which production of genetic variation is not a feature.
The method was first described by H. H. Lowe in 1927.
Pedigree selection is a breeding method in which the breeder keeps records of the ancestry of the cultivar.
The base population is established by crossing selected parents, followed by handling an actively segregating population.
Documentation of the pedigree enables breeders to trace parent–progeny back to an individual F2 plant from any subsequent generation.
The breeder should develop an effective, easy to maintain system of record keeping.
Pedigree selection is applicable to breeding species that allow individual plants to be observed, described, and harvested separately.
Clone is the progeny of a single plant, produced by asexual reproduction
Clonal selection is the selection of the most desirable members of a clone for continued vegetative propagation rather than for sexual reproduction.
The members of a clone keep up genetic constancy.
So by clonal selection and continued vegetative propagation, the desirable qualities of plants can be maintained for long.
Presentation on methods of plant breeding, classification of breeding methods, methods of breeding for self-pollinated,cross-pollinated and asexually propagated species, a brief account of breeding methods
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
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Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
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optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
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holes and slow-speed, highly variable, streams whose source regions are
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solar wind sources and understand what drives the complexity seen in the
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techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
2. Presented by
Patel Yogesh D.
M. Sc. (Agri.),2nd Semester
Reg. No. :04-AGRMA-01404-2015
“An
Assignment
on
“ Mass selection method in plant breeding’’
2
3. Mass selection refers to a methods of crop
improvement in which individual plants are
selected on the basis of phenotype from a mixed
population, their seeds are bulked and used to
grow next generation.
The oldest methods of crop improvement
Applicable to both self and cross pollinated
species.
Mass selection
4. 1) To increase the frequency of superior
genotypes from a genetically variable
population
2) Purify a mixed population with differing
phenotypes
3) Develop a new cultivar by improving the
average performance of the population
Objective
5. 1. Genetic constitution :-
Self pollinated crops:- A mass selected variety is
homozygous but heterogeneous because it is mixture of
several pure line.
Cross pollinated crops:- variety is mixture of homozygous
and heterozygous and variety is heterogeneous.
2. Adaptation:-
Mass selected varieties have wide adaptation.
Varieties are more stable against environmental changes.
Heterogeneity provides better buffering capacity.
Mass selected varieties have broader genetic base.
Adaptability is more in cross pollinated crops than in self
pollinated crops.
Main features
6. 3. Variation:-
They are composed of several pure line in self pollinated
crops and of several homo and heterozygous genotypes in
cross pollinated crops.
There is heritable variation in the mass selected varieties.
The heritable variation provides them good buffering
capacity.
4. Selection:-
Selection is effective due to presence of heritable variation.
5. Quality:-
A variety developed by mass selection is less uniform in the
quality of seed than pure lines due to presence of heritable
variation.
7. 6. Resistance :-
Mass selected varieties are less prone to the
attack of new disease due to genetic diversity.
They are more resistance or tolerant to new
disease.
7. Maintenance:-
Periodic removal of off type plants is
essential to maintain the yield of mass
selected varieties.
8. Used in both self and cross pollinated crops
Self pollinated:
• Mass selection variety is a mixture of several
pure lines.
• Constitutes a homozygous but heterogeneous
population.
Cross pollinated:
• A mass selected variety is mixture of several
homozygote and heterozygote.
• Variety is a heterozygous and heterogeneous.
Genetic Basis
9. • Positive mass selection:
Desirable plants are selected from a mixed population.
Their seeds are mixed together to grow further
generation.
This process is continued for several years.
Old varieties or land races are used as the base material.
• Negative mass selection:
Only undesirable off types of plants are removed from
the field and rest are allowed to grow.
Generally used for Varietal purification in seed
production.
Helps in maintaining high level of genetic purity.
Types of mass selection
10. The success of mass selection depends
on
Variability in base population.
Mode of inheritance of characters to be
improved.
Heritability of the characters.
Oligogenic recessive characters than
polygenic characters.
11. The local varieties are mixtures of several genotypes,
which may differ in flowering or maturity plant height,
disease resistant etc.
Many of these plants type would be inferior and low
yielding, such plants will be eliminated through mass
selection and local variety would be improved without
adversely affecting its adaptability and stability.
Because the new variety would be made up of the most
of the superior plants type present in the original local
variety.
1. Improvement of Desi or local variety
Application of mass selection.
12. 2. Purification the existing pure line variety.
Pure lines tend to become variable with time due
to mechanical mixtures, natural hybridization,
mutation etc. therefore, it is necessary that the
purity of pure line varieties be maintained
through regular mass selection.
Mass selection is generally important and
practised in cross-pollinated crop and has the
only limited application in self pollinated crop.
13. Selection of base population.
Selection of desirable plants from the base
population.
Mixing their seeds to raise next generation.
Evaluation in field trials.
Releasing as a new variety.
Steps of Mass selection:-
15. o o o o o o o o o
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First
year
second
year
Third to
fifth year
sixth
year
i. From a variable population, 200-2000 plants
with similar but desirable traits are selected
ii. The seed from selected plant are composited
i. The composited seed is planted in a
preliminary yield trial along with standard
checks
ii. Phenotype of the selected population is
clearly evaluated
i. Promising selections are evaluated in
coordinated yield trials at several locations
ii. It out standing raised as a new variety.
Seed multiplication for distribution.
16. Good method for old and land races varieties.
Good for purification of improved variety.
More stable then pure lines due to heterogeneity.
Good protection again disease and pest.
Simple and quick method for improvement.
Applicable in self and cross pollinated species.
MERIT
17. The developed through mass selection so variation and are not
uniform as pure line variety.
The improvement through mass selection is generally less than
that could be achieved through pure line selection.
In the absence of progeny test, it is not possible to determine if
the selected plant are homozygous.
Due to the popularity of pure line variety, mass selection is not
commonly use for improvement of self pollinated crop.
DEMERIT
18. Variety develop by mass selection are more
difficult to identified then pure line variety in seed
certification programmes.
Mass selection can not generated variability.
DEMERIT
20. 1. Rejection of inferior plants:- Inferior
plant are removed before flowering.
2. Use of composite pollen:-pollen are
collected then bulked.
Modification of mass selection
21. Also known as grid method of mass
selection
This method is suggested by Gardner in
1961
Field is divided into several small plots,
e.g., having 40-50 plants each.
Superior plant are selected in each
small plot.
Seeds are selected and composite to
raise the next generation.
3) Stratification of field-
22. A single cross hybrid, are planted as checks after
every one, two or four plants of the variety under
selection.
The yields of plants under selection are expressed as
under selection are expressed as per cent of the yield
of the yield of nearest check plant.
This scheme is designed to minimise the
environmental influence on the yields of plants
being selected.
And employs the principle of contiguous control.
4). Plant of single genotype
23. • Mass selection is based on only
phenotypic performance.
• Mass selection used in self pollinated as
well as cross pollinated crop.
• Mass selection is used for the
improvement of local or desi varieties.
Conclusion