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ASSIGNMENT
INTRODUCTION TO BIOCHEMISTRY
Topic:
Difference between Prokaryotic and Eukaryotic Cell
Submitted To:
Sir ShahidMaqbool
Submitted By:
11.fraz ahmad mazari
12. QuratUlAin17. Sidra Bakhtawar
13. AvimraHaleema18. Sidra Mazhar
14. Iqra Iqbal 19. Mariam Bashir
15. Ruhma 20. Romesa Arshad
M.Sc 1st
Semester (2018-20)
1
Difference between Prokaryotes & Eukaryotes
PROKARYOTES EUKARYOTES
Literal Meaning
The word originate from Greek term “PRO”
means “before”& “KARYO”means “nut or
kernel”.
The word originate from Greek term “EU”
means “true” & “KARYO” means “nut or
kernel”.
Definition
The unicellular organisms which do not
have membrane bound organelles like
nucleus & mitochondria are called
prokaryotes.
The unicellular or multicellular organisms
having membrane bound organelles like
nucleus &mitochondria are called eukaryotes.
Origin
The prokaryotes may have arisen more
than 3.5 billion years ago.
The eukaryotes have first appeared about 1.5
billion years ago.
Evolution
Living things have evolved into three large clusters of closelyrelated organisms called
Domains.
1-Archea 2-Bacteria 3-Eukarya
Cell Size
The size of prokaryotic cell is 1-10um. The size of eukaryotic cell is 10-100um.
Membrane Bound Organelles
Membrane bound organelles are absent in
prokaryotes because prokaryotic cell
membrane invaginated (inward folds) to
enclose copies of its genetic material.
Membrane bound organelles are present in
eukaryotes as the invagination in prokaryotic
membrane resulted in the formation of double
membrane bound entities (organelles) in a
single cell which then evolved into the
eukaryotic mitochondrion, nucleus
&2chloroplast.
Nucleus
True nucleus is absent in prokaryotes.
REASON: They lack membrane bound
organelles. Their genomic DNA is
concentrated and localized to a small area
within a cell (nucleoid). Nucleolus &
nuclear membrane are absent.
True nucleus is present in eukaryotes.
REASON: Membrane bound organelles are
evolved in the eukaryotes that’s why nucleus
is present. Nucleolus & nuclear membrane
are present.
Chromosomes
1-They have single circular chromosome
but also contain non-chromosomal DNA in
the form of plasmid.
2-They contain little amount of repetitive
DNA.
1-They have more than one linear
chromosomes & much more DNA 50 times
more than average prokaryotic cell.
2-They contain large amount of repetitive
DNA.
Ribosomes
Prokarotes have smaller 70S ribosome
made up of 50S & 30S subunit.
REASON: Prokaryotic ribosomes sediment
slower than eukaryotic ribosomes that’s
why they have small ribosome.
Eukaryotes have larger 80S ribosome in their
cytosol made up of 60S & 40S subunit.
REASON: Eukaryotic ribosomes sediment
faster than prokaryotic ribosome therefore,
they have large ribosome.
Mesosomes
Mesosomes in prokaryotes membrane are
areas that fold inward ,they play a role in
cellular respiration & physiological function.
Mesosomes are absent in eukaryotes
because majority of this process occurs in
mitochondria.
Lysosomes
Prokaryotes do not have
lysosomes,therefore, most digestion is
extracellular.They secrete enzymes to
breakdown molecules in the media
surrounding them & then they absorb te
products.
Lysosomes are present in eukaryotes that
contain digestive enzymes.They breakdown
worn-out cellular components or bacteria
engulfed by the cell.
Chloroplast
Chloroplast absent in prokaryotes,
chlorophyll scattered in the cytoplasm.
PHOTOSYNTHESIS: Prokaryotic
photosynthetic organisms have infoldings of
plasma membrane for chlorophyll
attachment & photosynthesis.
Chloroplast present in plants& algae having
lipid membranes. Chlorophyll present in
chloroplast. PHOTOSYNTHESIS:Eukaryotic
photosynthetic organisms carry out
photosynthesis in chloroplast.
Endoplasmic Reticulum
ER is absent in prokaryotes because there
is no compartmentalization. Mesosomes
perform function of ER in prokaryotes.
ER present in eukaryotesas they perform
function of biosynthesis & intracellular
transport.
Mitochondria
Mitochondria absent in prokaryotes because
it is membrane bound organelle therefore,
electron transport chain occur in
cytoplasmic membrane.
Mitochondria is present in eukaryotes
because membrane encased organelles are
present in them therefore, electron transport
chain located in the inner membrane of
mitochondria.
Cytoskeleton
All structural filaments containing Tubulin-
related protein & Actin-related protein
present in them, which provide shape and
also support cell division during binary
fission.
Cytoskeleton is present in eukaryotes
containing microtubules, actin microfilaments
& intermediate filament provide shape to cell,
allow movement of organelles within cell,
endocytosis & cell division.
Reproduction
1-Reproduction in prokaryotes is done by
genetic exchange material processes like
conjugation, transformation & transduction.
2-Organisms are haploid contain only a
1-All these processes are absent in
eukaryotes &genectic exchange occur through
sexual reproduction.
2-Organisms are diploid very rarely
single copy of genome. polyploids contain two copies of genome one
from each
parent.
Cell Division
Cell division in prokaryotes is done by
fission or budding.
Cell division in eukaryotes is done by mitosis
& meiosis.
Flagella
Submicroscopic in size, composed of only
one fiber.
Microscopic in size, membrane bound usually
arranged as nine doublets surrounding two
singlets.
Movement
Prokaryotes may have flagella each
composed of a single rotating fibril usually
not surrounding by membrane which
provide movement.
Eukaryotes may have flagella or cilia usually
surrounded by membrane (because
eukaryotic cell consist of distinct arrangement
of sliding microtubules) which provide
locomotion.
Cell Membrane
Prokaryotic cell membrane has few
phospholipids & lack cholesterol inside the
hydrophobic core.
Eukaryotic cell membrane has over 6
phospholipids & many other lipids.The
cholesterol is present regulating their fluidity.
Cell Wall
Cell wall in prokaryotes composed of
polysaccharides or peptidoglycan (domain
bacteria).
Reason: It is used to differentiate gram
+ve and gram –ve bacteria.
Cell wall in eukaryotes (if present) composed
of chitin (fungi) or cellulose (plants).Reason:
Gram staining techniques can not be
performed in Eukaryotes.
Gycocalyx
Glycocalyx in prokaryotes present as a
capsule or slime layer.
Glycocalyx in eukaryotes is present in
somecells that lack cell wall.
Other Process
1-Endocytosis, phagocytosis cytoplasmic
movement (cyclosis) are absent.
2-Communication system is not well
developed.
1-Endocytosis, phagocytosis &cyclosis are
present.
2-Communication system is well developed.
Examples
Bacteria (E.coli, streptococcus),
cyanobacteria (blue green algae), Archea.
Animals, Plants, algae, protozoans, fungi
(yeasts, molds, mushrooms).
REFERENCES:
https://en.wikipedia.org/wiki/Prokaryote
https://en.wikipedia.org/wiki/Eukaryote
www.lucidchart.com/
https://www.quora.com/What-are-prokaryotes-and-eukaryotes

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difference between prokaryotes and eukaryotes

  • 1. ASSIGNMENT INTRODUCTION TO BIOCHEMISTRY Topic: Difference between Prokaryotic and Eukaryotic Cell Submitted To: Sir ShahidMaqbool Submitted By: 11.fraz ahmad mazari 12. QuratUlAin17. Sidra Bakhtawar 13. AvimraHaleema18. Sidra Mazhar 14. Iqra Iqbal 19. Mariam Bashir 15. Ruhma 20. Romesa Arshad M.Sc 1st Semester (2018-20)
  • 2. 1 Difference between Prokaryotes & Eukaryotes PROKARYOTES EUKARYOTES Literal Meaning The word originate from Greek term “PRO” means “before”& “KARYO”means “nut or kernel”. The word originate from Greek term “EU” means “true” & “KARYO” means “nut or kernel”. Definition The unicellular organisms which do not have membrane bound organelles like nucleus & mitochondria are called prokaryotes. The unicellular or multicellular organisms having membrane bound organelles like nucleus &mitochondria are called eukaryotes. Origin The prokaryotes may have arisen more than 3.5 billion years ago. The eukaryotes have first appeared about 1.5 billion years ago. Evolution Living things have evolved into three large clusters of closelyrelated organisms called Domains. 1-Archea 2-Bacteria 3-Eukarya
  • 3. Cell Size The size of prokaryotic cell is 1-10um. The size of eukaryotic cell is 10-100um. Membrane Bound Organelles Membrane bound organelles are absent in prokaryotes because prokaryotic cell membrane invaginated (inward folds) to enclose copies of its genetic material. Membrane bound organelles are present in eukaryotes as the invagination in prokaryotic membrane resulted in the formation of double membrane bound entities (organelles) in a single cell which then evolved into the eukaryotic mitochondrion, nucleus &2chloroplast. Nucleus True nucleus is absent in prokaryotes. REASON: They lack membrane bound organelles. Their genomic DNA is concentrated and localized to a small area within a cell (nucleoid). Nucleolus & nuclear membrane are absent. True nucleus is present in eukaryotes. REASON: Membrane bound organelles are evolved in the eukaryotes that’s why nucleus is present. Nucleolus & nuclear membrane are present. Chromosomes
  • 4. 1-They have single circular chromosome but also contain non-chromosomal DNA in the form of plasmid. 2-They contain little amount of repetitive DNA. 1-They have more than one linear chromosomes & much more DNA 50 times more than average prokaryotic cell. 2-They contain large amount of repetitive DNA. Ribosomes Prokarotes have smaller 70S ribosome made up of 50S & 30S subunit. REASON: Prokaryotic ribosomes sediment slower than eukaryotic ribosomes that’s why they have small ribosome. Eukaryotes have larger 80S ribosome in their cytosol made up of 60S & 40S subunit. REASON: Eukaryotic ribosomes sediment faster than prokaryotic ribosome therefore, they have large ribosome. Mesosomes Mesosomes in prokaryotes membrane are areas that fold inward ,they play a role in cellular respiration & physiological function. Mesosomes are absent in eukaryotes because majority of this process occurs in mitochondria. Lysosomes Prokaryotes do not have lysosomes,therefore, most digestion is extracellular.They secrete enzymes to breakdown molecules in the media surrounding them & then they absorb te products. Lysosomes are present in eukaryotes that contain digestive enzymes.They breakdown worn-out cellular components or bacteria engulfed by the cell.
  • 5. Chloroplast Chloroplast absent in prokaryotes, chlorophyll scattered in the cytoplasm. PHOTOSYNTHESIS: Prokaryotic photosynthetic organisms have infoldings of plasma membrane for chlorophyll attachment & photosynthesis. Chloroplast present in plants& algae having lipid membranes. Chlorophyll present in chloroplast. PHOTOSYNTHESIS:Eukaryotic photosynthetic organisms carry out photosynthesis in chloroplast. Endoplasmic Reticulum ER is absent in prokaryotes because there is no compartmentalization. Mesosomes perform function of ER in prokaryotes. ER present in eukaryotesas they perform function of biosynthesis & intracellular transport. Mitochondria Mitochondria absent in prokaryotes because it is membrane bound organelle therefore, electron transport chain occur in cytoplasmic membrane. Mitochondria is present in eukaryotes because membrane encased organelles are present in them therefore, electron transport chain located in the inner membrane of mitochondria. Cytoskeleton All structural filaments containing Tubulin- related protein & Actin-related protein present in them, which provide shape and also support cell division during binary fission. Cytoskeleton is present in eukaryotes containing microtubules, actin microfilaments & intermediate filament provide shape to cell, allow movement of organelles within cell, endocytosis & cell division. Reproduction 1-Reproduction in prokaryotes is done by genetic exchange material processes like conjugation, transformation & transduction. 2-Organisms are haploid contain only a 1-All these processes are absent in eukaryotes &genectic exchange occur through sexual reproduction. 2-Organisms are diploid very rarely
  • 6. single copy of genome. polyploids contain two copies of genome one from each parent. Cell Division Cell division in prokaryotes is done by fission or budding. Cell division in eukaryotes is done by mitosis & meiosis. Flagella Submicroscopic in size, composed of only one fiber. Microscopic in size, membrane bound usually arranged as nine doublets surrounding two singlets. Movement Prokaryotes may have flagella each composed of a single rotating fibril usually not surrounding by membrane which provide movement. Eukaryotes may have flagella or cilia usually surrounded by membrane (because eukaryotic cell consist of distinct arrangement of sliding microtubules) which provide locomotion. Cell Membrane Prokaryotic cell membrane has few phospholipids & lack cholesterol inside the hydrophobic core. Eukaryotic cell membrane has over 6 phospholipids & many other lipids.The cholesterol is present regulating their fluidity. Cell Wall Cell wall in prokaryotes composed of polysaccharides or peptidoglycan (domain bacteria). Reason: It is used to differentiate gram +ve and gram –ve bacteria. Cell wall in eukaryotes (if present) composed of chitin (fungi) or cellulose (plants).Reason: Gram staining techniques can not be performed in Eukaryotes. Gycocalyx
  • 7. Glycocalyx in prokaryotes present as a capsule or slime layer. Glycocalyx in eukaryotes is present in somecells that lack cell wall. Other Process 1-Endocytosis, phagocytosis cytoplasmic movement (cyclosis) are absent. 2-Communication system is not well developed. 1-Endocytosis, phagocytosis &cyclosis are present. 2-Communication system is well developed. Examples Bacteria (E.coli, streptococcus), cyanobacteria (blue green algae), Archea. Animals, Plants, algae, protozoans, fungi (yeasts, molds, mushrooms). REFERENCES: https://en.wikipedia.org/wiki/Prokaryote https://en.wikipedia.org/wiki/Eukaryote www.lucidchart.com/ https://www.quora.com/What-are-prokaryotes-and-eukaryotes