This document discusses diagnostic tests for COVID-19. It describes how samples are collected, typically nasopharyngeal or oropharyngeal swabs. Real-time reverse transcription polymerase chain reaction (RT-PCR) is the preferred testing method, using RNA extraction and fluorescent markers to detect viral DNA. Lateral flow and ELISA tests detect antibodies produced in response to infection. Treatment options discussed include chloroquine and favilavir. Several vaccine candidates are under development at universities and companies.
Coronavirus disease 2019 (COVID-19). Complete information on coronavirus. Introduction, history, symptoms, covid19 structure, S protein of coronavirus, M proteins of coronavirus, spreading variations of coronavirus, vaccines, drugs to control coronavirus.
The PPT is mainly all about Mycobacterium Tuberculosis. Agents causing the disease Tuberculosis, pathogenesis, laboratory diagnosis, treatment and prophylaxis. It was made for both BSc and MSc students.
Coronavirus disease 2019 (COVID-19). Complete information on coronavirus. Introduction, history, symptoms, covid19 structure, S protein of coronavirus, M proteins of coronavirus, spreading variations of coronavirus, vaccines, drugs to control coronavirus.
The PPT is mainly all about Mycobacterium Tuberculosis. Agents causing the disease Tuberculosis, pathogenesis, laboratory diagnosis, treatment and prophylaxis. It was made for both BSc and MSc students.
COVID-19 I Coronavirus Disease I Harshit JadavHarshit Jadav
This presentation highlights all the basic information regarding the current pandemic COVID-19.
This presentation includes an introduction, recent stats, the structure of coronavirus, pathogenesis of coronavirus, diagnosis, treatment and preventive measures of COVID-19
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS).
herpes simplex virus is a double stranded DNA virus causing many symptoms all over the body. it affects globally all over the world .
neonatal hsv attacks even the baby and made them to a fatal conditions.
COVID-19 I Coronavirus Disease I Harshit JadavHarshit Jadav
This presentation highlights all the basic information regarding the current pandemic COVID-19.
This presentation includes an introduction, recent stats, the structure of coronavirus, pathogenesis of coronavirus, diagnosis, treatment and preventive measures of COVID-19
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS).
herpes simplex virus is a double stranded DNA virus causing many symptoms all over the body. it affects globally all over the world .
neonatal hsv attacks even the baby and made them to a fatal conditions.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
LABORATORY DIAGNOSIS OF VIRAL INFECTIONS.pdfWani Insha
Laboratory diagnosis of viral infections is useful for the following purposes:
To start antiviral drugs for those viral infections for which specific drugs are available such as herpes, CMV, HIV, influenza and respiratory syncytial virus (RSV)
Screening of blood donors for HIV, hepatitis B and hepatitis C-helps in prevention of transfusion transmitted infections
Surveillance purpose: To assess the disease burden in the community by estimating the prevalence and incidence of viral infections
For outbreak or epidemic investigation, e.g. influenza epidemics, dengue outbreaks-to initiate appropriate control measures
To start post-exposure prophylaxis of antiretroviral drugs to the health care workers following needle stick injury.
To initiate certain measures: For example,
If rubella is diagnosed in the first trimester of pregnancy, termination of pregnancy is recommended
If newborn is diagnosed to have hepatitis B infection, then immunoglobulins (HBIG) should be started within 12 hours of birth.
A pneumonia of unknown cause detected in Wuhan, China was first reported to the WHO
Country Office in China on 31 December 2019.In the last Nine months, almost Ten lakhs of
lives have already been Death, around three billion of people are in quarantine, and global
economies have been decreased. The outbreak of pandemic Covid-19 all over the world has
broken down the political, social, economic, religious and financial structures of the whole
world. The World’s top economies country such as the Australia, USA, India China, UK,
Germany, France, Italy, Japan and many others. The Stock Markets around the world have
been broken down and oil prices have fallen off a cliff. A report was published on BBC where
they describe every single week 3.3 million Americans have been unemployment and a week
later another 6.6 million people started searching for new jobs. The novel coronavirus is a
microscopic organism that has become an epidemic over time around the world. The United
States, Europe, Britain, Italy, Spain and France have already been hit by the virus. These
countries have already become mortal by Corona virus.
Introduction to coviself antibody testing kitAnneHathaway6
Meanwhile, the RT-PCR test requires a healthcare provider to take a sample and send it to a laboratory for analysis. In the sample, the analysis can find evidence of the virus's genetic material. If it detects two distinct SARS-CoV-2 genes, this test can confirm a COVID-19 diagnosis. It will yield an inconclusive result if it only finds one.
COVID-19: Biology, Transmission, and DetectionAlejandroAlRuiz
A short PowerPoint for those interested in learning more about the biology of SARS-CoV-2, how it is transmitted, and what are the current methods of detection. This PowerPoint also demonstrates how we can begin to teach about SARS-CoV-2 in our classrooms.
lab diagnosis of viral infections - mayuri.pptxDrmayuribhise
T.M. River, 1937
Modified from Koch’s Postulates (proof of bacterial diseases)
Isolate virus from diseased hosts.
Cultivation of virus in host cells.
Proof of filterability.
Production of a comparable disease when the cultivated virus is used to infect experimental animals.
Reisolation of the same virus from the infected experimental animal.
Detection of a specific immune response to the virus.
Much more expensive and difficult to study animal viruses than bacteriophages
Cultivation in host cells
Living animal
Embryonated chicken eggs
Cell or tissue culture (= in vitro)
Over 60% of all infectious disease cases seen by a physician are due to viral infections.
Quality of patient specimens and their transport to the laboratory is importantViral Diagnostics in the Clinical Laboratory
Types of specimens:-
Respiratory tract infections: Nasal and bronchial washings, throat and nasal swabs, sputum
Eye infections: throat and Conjunctival swab/scraping
Gastrointestinal tract infections: stool and rectal swabs
Vesicular rash: vesicle fluid, skin scrapings
Maculopapular rash: throat, stool, and rectal swabs
CNS (encephalitis and meningitis cases): stool, tissue, saliva, brain biopsy, cerebrospinal fluid
Genital infections: vesicle fluid or swab
Urinary tract infections: urine
Blood borne infections: blood
Sterile, leak proof container
Minimal interval
Transport media
Viral infusion broth (VIB)
Sucrose-phosphate-glutamate (SPG)
Storage temperature:
4 deg C for up to 96 hours
Minus 70 deg C beyond 96 hours
Repeated cycles of freezing and thawing to be avoided
106 virus particles per ml required for visualization,
50,000 - 60,000 magnification normally used.Specimens are negatively stained by Potassium phosphotungstate and scanned under EM
Viruses may be detected in the following specimens.
Virus particles are detected and identified on the basis of morphology.
A) Shape
Rabiesvirus –bullet shaped
Rotavirus –Cart wheel
Coronavirus –petal shaped peplomers
Adenovirus –space vehicle shaped
Astrovirus ---Star shaped
B) Direct detection from specimens
For viruses that are difficult to cultivate ,EM can be used as primary tool for diagnosis
Faeces Rotavirus, Adenovirus
Norwalk like viruses
Astrovirus, Calicivirus
Vesicle Fluid HSV
VZV
Skin scrapings papillomavirus, orf
molluscum contagiosum
As an alternative to tissue culture
As tissues culture is time consuming and technically demanding ,EM is used as an alternative :-
1) Vesicular rashes –HSV and VZV detection from vesicular fluid
2) Meningitis—Detection of enterovirus and mumps from CSF.
Virus detection from tissue cultures EM can be used for detection of viral growth in tissue culture
The sensitivity and specificity of EM can be improved by adding specific antiviral antibody to the specimen to aggregate the virus particles which can be centrifuged
The sediment is negatively stained and viewed under EM
Direct immumofluroscence
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
3. What is COVID19?
COVID-19 – Coronavirus disease-19
A respiratory illness caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2)
Corona has crown-like spikes on the outer surface of the virus
Coronaviruses are minute in size (65–125 nm in diameter)
Single-stranded RNA as a nucleic material, size
Why named Corona Virus?
4. What is structural characteristics?
Spike protein: S - proteins are functionally
divided into two subunits (S1 and S2).
S1 domain: Responsible for receptor
binding to receptor on target cell
S2 domain: Responsible for fusion
N protein : plays an important role in viral
pathogenesis, replication, and RNA
packaging
CoV replication cycle
Membrane (M) protein: M protein is the most abundant structural protein and defines
the shape of the viral envelope. glycosylated in the Golgi apparatus
Envelope (E) protein: the smallest of the major structural proteins , E is abundantly
expressed inside the infected cell,
Role in the assembly and morphogenesis of virions within the cell.
6. Diagnostics procedure
How paramedical staff will diagnosis of infection caused by
SARS -CoV -2?
Q. What are the percussion soluble be taken for collection of
sample?
Sample Collection
Ans. Collecting the proper respiratory tract specimen at the right
time from the right anatomic site
How to collect sample?
Within 5 – 6 days of the onset of symptoms when high viral
load s in their upper and lower respiratory tract
7. A nasopharyngeal (NP) swab or an oropharyngeal (OP) swab are
often recommended
OP swabs were used much more frequently in China
But
SARS -CoV -2 RNA was detected only in 32% of OP swabs.
Nasopharyngeal (NP) is Best.
The swab must be inserted deeply into the nasal cavity and should
elicit “tears
Where the swab to be inserted?
8. Swabs should be kept in place for 10 seconds while twirling the
swab three times
Personal Protective Equipment (PPE )
Swabs should be placed in viral (universal) transport
medium for rapid transportation to the clinical microbiology
laboratory
Bronchoalveolar lavageOther Sample ????
Sputum- Lower respiratory specimens
Yielded the highest SARS -CoV -2 RNA rate
Virus has been detected in blood and stool,
9. Safety Measures for Specimen Processing
should be done in a class II biological
safety cabinet
Safety cabinet should be in a negative
pressure room within the laboratory
11. Methods of Testing
Laboratory
Diagnosis
Real -time
RT -PCR assays
Lateral flow
assays
Enzyme-Linked
Immunosorbent
Assay
RT -PCR) test method: preferred testing method for the
diagnosis of COVID19
12. Processing of respiratory specimen for nucleic acid extraction
before real -time RT -PCR
Real-time reverse-transcription polymerase chain
reaction (RT-PCR)
The specimen should be transferred to
lysis buffer
Lysis buffer = guanidinium -based
inactivating agent as well as a non -
denaturing detergent.
13. Real-time reverse-transcription polymerase chain
reaction (RT-PCR)
RNA extraction from Virus: self -enclosed systems integrating
nucleic acid extraction (GeneXpert®)
Used as a frontline test for COVID-19 as it directly tests for the
presence of the virus RNA.
Sensitive and reliable, capable of producing results in 3-4 hours
Occurs in a closed system to minimize false -positive results
14. Real-time reverse-transcription polymerase chain
reaction (RT-PCR)
Positive -sense, single -stranded RNA genome that can be used for PCR
assays
The test kit enzymes copy the RNA to DNA, which is amplified to allow
virus detection by using a PCR machine.
35 billion copies of viral DNA are made for each viral RNA strand
Fluorescent markers are typically used to bind to the amplified DNA and
produce light, which can be read by the machine to produce the test result
15. Real-time reverse-transcription polymerase chain
reaction (RT-PCR)
A cycle threshold value (Ct -value) less than 40 is defined as a
positive test
RT-PCR is accepted by scientists and medical staff as a robust and well
documented technique.
16. Lateral Flow / Colloidal Gold
Immunochromatography
‘Antibody tests’ ----- used for pregnancy tests.
Currently used to detect antibodies to disease in a
patient's blood
Tests can detect antibody to virus from patient
blood
Detect the presence of active virus by detecting
virus proteins directly
Require a drop of patient blood
Ability to see if patients are currently infected or
have recovered from COVID-19
17. Lateral Flow / Colloidal Gold Immunochromatography
Label (Enzymes or nanoparticles or fluorescence dyes) coated antibody or aptamer
is immobilized at conjugate pad.
A primary antibody or aptamer against target analyte is immobilized over test line.
A secondary antibody or probe against labeled conjugate antibody/aptamer is
immobilized at control zone.
18. Lateral Flow / Colloidal Gold Immunochromatography
The antibody IgG and IgM lateral flow immunoassay tests are
very simple to read:
Test lines will appear if either of the antibody types are found in the sample. The
appearance of lines for IgG or IgM, or both indicate a positive test
19. Enzyme-Linked Immunosorbent Assay (ELISA)
An Enzyme-Linked Immunosorbent Assay (ELISA) is a common biochemical
technique that can be used to detect antigens or antibodies
ELISAs use enzymes linked to antibodies that can attach to the molecule
that is being tested for and cause a colour change that can be measured by
a specialised machine (ELISA Reader)
Allowing cheap and time effective method for batch testing of large numbers
of patient
The most effective ELISA assays in monitoring prior infection detect
antibodies against SARS-CoV-2.
An ELISA detects antibodies produced in patient blood due to infection
with SARS-CoV-2.
20. Enzyme-Linked Immunosorbent Assay (ELISA)
1. A patient sample of blood or serum
2. The patient antibodies to SARS-CoV-2 stick to the SARS-CoV-2 proteins coated on the
bottom
3. Antibodies with enzymes attached are added and stick to patient antibodies present in
the well
4. Special colourless molecule is added to the well.
Interpretation: An antibody test using ELISA would show a positive result (colour
change) if the patient has antibodies to COVID-19.
21. Drug used for treatment
Chloroquine approved for emergency use by US
FDA
(hydroxychloroquine/Plaquenil, used to treat malaria
and arthritis)
Favilavir, the first approved coronavirus drug in
China
An anti-viral drug, as a treatment for coronavirus.
22. Vaccine under development
University of Oxford’s ChAdOx1 nCoV-19 is an
adenovirus vaccine vector developed by the
university’s Jenner Institute
Alabama at Birmingham (UAB) to develop a single
dose intranasal vaccine for COVID-19 named
AdCOVID.
DNA vaccine by Entos Pharmaceuticals