CRISPR technology -A revolutionary discovery
•Download as PPTX, PDF•
8 likes•1,529 views
CRISPR technology allows for genome editing using a prokaryotic immune system called CRISPR/Cas. The system works by adapting spacers from viral DNA, producing CRISPR RNA, and targeting matching sequences. It is being applied in industry to make bacterial cultures virus-resistant, in labs for genetic engineering, and in medicine for treating genetic diseases and developing more specific antibiotics.
Report
Share
Report
Share
Recommended
Crispr cas9
CRISPR-Cas9 is a genome editing technique that allows DNA to be precisely cut and modified. It involves using the Cas9 enzyme, guided by RNA, to cut DNA at a specific target location. The cell's DNA repair machinery can then introduce changes by adding, removing, or replacing DNA segments. CRISPR-Cas9 was adapted from a natural defense system in bacteria against viruses. It holds promise for treating genetic diseases but also raises ethical concerns about editing human embryos or germline cells.
Crispr cas9
The document provides an introduction to the CRISPR/Cas9 genome editing technique. It discusses that CRISPR/Cas9 uses guide RNAs to direct the Cas9 nuclease to cut DNA at specific locations, and this double strand break can be repaired through nonhomologous end joining or homology directed repair to knock out or knock in genes. It also explains that CRISPR/Cas9 is more efficient, less expensive, and easier to use than previous genome editing techniques like ZFNs and TALENs. The document outlines several applications of CRISPR/Cas9 in biomedical research areas such as immunology, stem cell research, and generating transgenic animals.
Crispar
This document provides an overview of the CRISPR-Cas immune system in bacteria and its applications. It discusses:
1. The history and components of the CRISPR-Cas system, including Cas proteins, CRISPR RNA, and protospacer adjacent motifs.
2. The three main types of CRISPR-Cas systems and their mechanisms of targeting DNA or RNA.
3. How engineered versions of Cas9 can be used for targeted genome editing and modulation of gene expression in mammalian cells.
4. Applications of CRISPR-Cas in microbiology such as genetic engineering of bacteria, gene repression/activation using deactivated Cas9, and developing sequence-specific antimicrobials.
SEMINAR ON CRISPR
1. CRISPR is a bacterial immune system that provides immunity against viruses. It works by matching DNA sequences from viruses to DNA spacers and cleaving invading DNA.
2. The type II CRISPR system is the most well studied and requires only three components - Cas9 protein, CRISPR RNA (crRNA), and trans-activating CRISPR RNA (tracrRNA) - to function. Combining crRNA and tracrRNA into a single RNA molecule called sgRNA was crucial for developing the CRISPR technique.
3. CRISPR technology has become a powerful genome editing tool due to its simplicity, high efficiency, low cost, and ease of use. It allows targeted
Crispr-Cas9 technology
Crispr-Cas9 system works on the concept of bacterial defence mechanism. The idea of which was replicated in eukaryotic cell in in- vitro condition by the researchers.
Crispr
This document discusses the CRISPR-Cas9 genome editing technology. It begins by explaining that genome editing involves inserting, deleting, or replacing DNA in a genome using engineered molecular scissors. It then describes CRISPR-Cas9 specifically, noting that it is a bacterial immune system that utilizes CRISPR sequences and Cas9 proteins to cut viral DNA. The document outlines the components of the CRISPR-Cas9 system, including guide RNAs that locate DNA sections and Cas9 proteins that modify the DNA. It also discusses applications of CRISPR-Cas9 such as curing genetic diseases and editing crops, as well as noting that further investigation is still needed to ensure its safe therapeutic use.
Crispr
This document summarizes a presentation on CRISPR/Cas genome editing. It defines CRISPR/Cas as a type of genetic engineering that uses artificially engineered nucleases to make specific cuts in DNA. It describes the CRISPR/Cas system's origins and components, including Cas9, guide RNA, and PAM sequences. Applications discussed include genome editing in animals and plants, as well as concerns over off-target effects. Companies offering CRISPR services or kits are also mentioned.
Crispr cas ppt by ashish
The document summarizes the CRISPR-Cas immune system. It discusses how CRISPR-Cas systems use clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins to recognize and cleave foreign DNA. The systems acquire spacers from invading viruses and phages and integrate them into the CRISPR loci to develop immunity. The CRISPR-Cas system has three stages - adaptation, expression and interference. It also discusses applications of CRISPR-Cas9 in genome editing and modulation of gene expression.
Recommended
Crispr cas9
CRISPR-Cas9 is a genome editing technique that allows DNA to be precisely cut and modified. It involves using the Cas9 enzyme, guided by RNA, to cut DNA at a specific target location. The cell's DNA repair machinery can then introduce changes by adding, removing, or replacing DNA segments. CRISPR-Cas9 was adapted from a natural defense system in bacteria against viruses. It holds promise for treating genetic diseases but also raises ethical concerns about editing human embryos or germline cells.
Crispr cas9
The document provides an introduction to the CRISPR/Cas9 genome editing technique. It discusses that CRISPR/Cas9 uses guide RNAs to direct the Cas9 nuclease to cut DNA at specific locations, and this double strand break can be repaired through nonhomologous end joining or homology directed repair to knock out or knock in genes. It also explains that CRISPR/Cas9 is more efficient, less expensive, and easier to use than previous genome editing techniques like ZFNs and TALENs. The document outlines several applications of CRISPR/Cas9 in biomedical research areas such as immunology, stem cell research, and generating transgenic animals.
Crispar
This document provides an overview of the CRISPR-Cas immune system in bacteria and its applications. It discusses:
1. The history and components of the CRISPR-Cas system, including Cas proteins, CRISPR RNA, and protospacer adjacent motifs.
2. The three main types of CRISPR-Cas systems and their mechanisms of targeting DNA or RNA.
3. How engineered versions of Cas9 can be used for targeted genome editing and modulation of gene expression in mammalian cells.
4. Applications of CRISPR-Cas in microbiology such as genetic engineering of bacteria, gene repression/activation using deactivated Cas9, and developing sequence-specific antimicrobials.
SEMINAR ON CRISPR
1. CRISPR is a bacterial immune system that provides immunity against viruses. It works by matching DNA sequences from viruses to DNA spacers and cleaving invading DNA.
2. The type II CRISPR system is the most well studied and requires only three components - Cas9 protein, CRISPR RNA (crRNA), and trans-activating CRISPR RNA (tracrRNA) - to function. Combining crRNA and tracrRNA into a single RNA molecule called sgRNA was crucial for developing the CRISPR technique.
3. CRISPR technology has become a powerful genome editing tool due to its simplicity, high efficiency, low cost, and ease of use. It allows targeted
Crispr-Cas9 technology
Crispr-Cas9 system works on the concept of bacterial defence mechanism. The idea of which was replicated in eukaryotic cell in in- vitro condition by the researchers.
Crispr
This document discusses the CRISPR-Cas9 genome editing technology. It begins by explaining that genome editing involves inserting, deleting, or replacing DNA in a genome using engineered molecular scissors. It then describes CRISPR-Cas9 specifically, noting that it is a bacterial immune system that utilizes CRISPR sequences and Cas9 proteins to cut viral DNA. The document outlines the components of the CRISPR-Cas9 system, including guide RNAs that locate DNA sections and Cas9 proteins that modify the DNA. It also discusses applications of CRISPR-Cas9 such as curing genetic diseases and editing crops, as well as noting that further investigation is still needed to ensure its safe therapeutic use.
Crispr
This document summarizes a presentation on CRISPR/Cas genome editing. It defines CRISPR/Cas as a type of genetic engineering that uses artificially engineered nucleases to make specific cuts in DNA. It describes the CRISPR/Cas system's origins and components, including Cas9, guide RNA, and PAM sequences. Applications discussed include genome editing in animals and plants, as well as concerns over off-target effects. Companies offering CRISPR services or kits are also mentioned.
Crispr cas ppt by ashish
The document summarizes the CRISPR-Cas immune system. It discusses how CRISPR-Cas systems use clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins to recognize and cleave foreign DNA. The systems acquire spacers from invading viruses and phages and integrate them into the CRISPR loci to develop immunity. The CRISPR-Cas system has three stages - adaptation, expression and interference. It also discusses applications of CRISPR-Cas9 in genome editing and modulation of gene expression.
Crispr application
With the excellent CRISPR/Cas9 platform and experienced scientists, our well-trained staff successfully finishes scores of custom gene editing projects every year.
https://www.creative-biogene.com/crispr-cas9/
crispr cas 9
CRISPR-Cas9 is a gene editing technology that uses the bacterial immune system to cut DNA at specific locations. It allows researchers to understand, characterize, and control DNA. CRISPR-Cas9 uses an RNA-guided DNA endonuclease enzyme called Cas9 that is directed by guide RNA to cleave target DNA. It has numerous applications including modifying genes in plants and animals, developing disease resistant crops, and potentially curing genetic diseases in humans by precisely editing genes. While revolutionary, it also raises ethical concerns that must be considered and addressed.
Genome editing
genome editing is the emerging branch which includes the specific and precise engineering of genomic regions
Crispr cas: A new tool of genome editing
The document summarizes a presentation on CRISPR cas9, a new genome editing tool. It discusses the history of CRISPR, how CRISPR functions in bacteria, the classification and components of CRISPR systems, and the mechanism of CRISPR cas9. It then covers applications of CRISPR cas9 in genome editing, databases of CRISPR sequences, case studies using the technology, and future directions of CRISPR research.
Crispr cas
This document summarizes Jai Kishan's presentation on gene manipulation techniques. It discusses conventional plant breeding, mutation breeding, transgenic plants, and several precise gene editing techniques like ZFN, TALENs, and CRISPR-Cas9. CRISPR-Cas systems provide adaptive immunity in bacteria and archaea by recognizing foreign DNA. The document then reviews the history and components of the CRISPR-Cas system and its applications in cancer therapy, viral diseases, bacterial infections, and genetic disorders. It also discusses challenges like delivery methods, mosaicism, immune responses, and off-target mutations. Key references on the discovery of CRISPR and its use in agriculture and as a genetic manipulation tool are presented.
Crispr cas 9 presentation
CRISPR Cas-9 is a genome editing technology that uses a bacterial immune system mechanism to allow permanent modification of genes within organisms. It involves clustered regularly interspaced short palindromic repeats (CRISPRs) found in bacterial genomes that play a key role in defense against viruses. The Cas9 protein has six domains including two nuclease domains that cut single-stranded DNA and allow it to be used for genome editing applications like gene silencing, DNA-free editing, and homology-directed repair.
Crispr cas9 scalpels and their application
This document provides information on CRISPR Cas9 genome editing. It discusses the history and discovery of CRISPR dating back to 1987. It describes the key components of the CRISPR Cas9 system including Cas9 proteins, CRISPR RNA, protospacers, and PAM sequences. The mechanisms of how CRISPR Cas9 edits genomes through double strand breaks is explained. Finally, applications of CRISPR Cas9 are summarized, including using it to correct genetic mutations causing diseases in animals and potential applications in humans.
CRISPR-Cas9 system a tool for gene editing presentation
CRISPR Cas9 System for Gene Editing
The document summarizes CRISPR Cas9 gene editing. It discusses the timeline of CRISPR discoveries from 1987-2019. It describes the classification, structure, and mechanism of the CRISPR Cas9 system. Applications discussed include using CRISPR in bacteria, wheat, and to alter muscle mass in humans. Ethical concerns regarding uses in human embryos and potential for non-medical enhancement are also covered.
Crispr cas9
CRISPR, CAS9, Genome Editing, ZFNS, TALENS, Spacer DNA, tracrRNA, crRNA, sgRNA, PAM, CRISPAR-CAS9, CAS GENES, Gene editing, gene silencing, gene knockout, CAS protein, enzyme, nuclease, restriction enzyme, CRISPR Array, Genetic Engineering, Applications of CRISPR, Advantages of CRISPR, LIMITATIONS OF CRISPR, DISADVANTAGES OF CRISPR, BENEFITS OF CRISPR, Future of CRISPR, CRISPR and Cancer, Ethical Issues.
CRISPR-Cas9 mediated genome editing : A comprehensive review with zebrafish a...
CRISPR-Cas9 mediated genome editing : A comprehensive review with zebrafish a...Arkaprava Roychaudhury
This document provides an overview of the CRISPR-Cas9 genome editing system. It discusses the history and mechanism of genome editing, describes the key components and mechanism of CRISPR-Cas9, and outlines its applications in research including in animal models like zebrafish. The document also discusses advantages and disadvantages of CRISPR-Cas9 as well as ethical issues and future prospects of this genome editing technique.Crispr cas9 technology
This document provides an overview of CRISPR-Cas9 technology. It defines CRISPR as DNA sequences in bacteria that contain snippets of viral DNA used to detect and destroy invading viruses. The CRISPR system contains Cas9 proteins that cut DNA at specific locations guided by RNA, and RNA guide molecules. It works by integrating viral DNA into the bacteria's CRISPR loci, which are then transcribed into RNA to guide Cas9 to cleave invading viral DNA. Applications of CRISPR include disease modeling, cancer research, and correcting mutations in human embryos.
Crispr cas9
This document discusses the CRISPR-Cas9 genome editing technique. It begins with an overview of genome editing and provides a brief history. It then focuses on explaining CRISPR-Cas9, including its key components, how it was discovered as a natural bacterial immune system, and how it functions as a genomic tool. The document outlines the general CRISPR-Cas9 protocol and recent advances in the technique. It discusses applications in agriculture and for diseases. It also touches on advantages and limitations, as well as ethical issues. Two case studies are provided that demonstrate using CRISPR-Cas9 to modify genes in rice plants.
Crispr cas 9
This document provides information about CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) including:
1) CRISPR is a genome editing technique that uses the Cas9 enzyme to cut DNA at a targeted location, allowing DNA inserts or modifications.
2) It was originally discovered as a bacterial immune system but is now the most widely used approach for genome engineering due to its simplicity, precision, and versatility.
3) There is an ongoing patent dispute between researchers Jennifer Doudna, Emmanuelle Charpentier, and Feng Zhang over who first demonstrated using CRISPR-Cas9 for genome editing in human cells.
Crispr/cas9 101
This document provides an overview of CRISPR/Cas9 genome editing. It discusses the history and limitations of prior genome engineering techniques like recombinant DNA and zinc finger nucleases. It then explains how CRISPR/Cas9 works as a RNA-guided DNA endonuclease and how this allows it to efficiently and specifically edit genomes. The document outlines several applications of CRISPR/Cas9 like generating knockout animals and cell lines. It also notes some concerns about using the technique for human genome editing.
CRISPR Technology
A simple version of the CRISPR/Cas system, CRISPR/Cas9, has been modified to edit genomes. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added.
Crispr
This document summarizes a seminar given on CRISPR technology. It discusses the history of CRISPR research from its discovery in bacteria in 1987 to its development as a genome editing tool in 2012. Key events and applications are outlined, including the founding of early biotech companies utilizing CRISPR. The core concepts of how CRISPR induces double strand breaks and DNA repair are explained. Recent advances in engineered Cas9 variants and discovery of Cpf1 are also summarized.
CRISPR-Cas systems and applications
i explained about basics of genome engineering and crispr system.
CRISPR will change the world and it is just the beginning, are you ready to meet the future? you think its great and beautiful or.....?
please give your feedback to my email
pooyanaghshbandi@yahoo.com
i am starting to write a critical and fantastic review article about CRISPR, if you are interested to join please contact me.
Crispr handbook 2015
This handbook describes CRISPR/Cas9 genome editing and other research applications for CRISPR technology.
CRISPR-Cas system
CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found within the genomes of prokaryotic organisms such as bacteria and archaea. These sequences are derived from DNA fragments of bacteriophages that have previously infected the prokaryote and are used to detect and destroy DNA from similar phages during subsequent infections. Hence these sequences play a key role in the antiviral defense system of prokaryotes.
Cas9 (CRISPR-associated protein 9) is an enzyme that uses CRISPR sequences as a guide to recognize and cleave specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within organisms.This editing process has a wide variety of applications including basic biological research, development of biotechnology products, and treatment of diseases.
The CRISPR-Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as those present within plasmids and phages that provides a form of acquired immunity. RNA harboring the spacer sequence helps Cas (CRISPR-associated) proteins recognize and cut foreign pathogenic DNA. Other RNA-guided Cas proteins cut foreign RNA. CRISPR are found in approximately 50% of sequenced bacterial genomes and nearly 90% of sequenced archaea.
An Introduction to Crispr Genome Editing
In this short presentation, I make a case for doing genome editing vs some of the approaches that have gone before, describe some of the tools available, and the focus on CRISPR-Cas9, what it is, where it's come from and how it works.
the application of CRISPR/Cas9 system in genome editing
CRISPR-cas9 system is a new robust genome editing tool and also this is a really easy to use system.
کاربرد سیستم کریسپر در ویرایش ژنوم.ppt
More Related Content
What's hot
Crispr application
With the excellent CRISPR/Cas9 platform and experienced scientists, our well-trained staff successfully finishes scores of custom gene editing projects every year.
https://www.creative-biogene.com/crispr-cas9/
crispr cas 9
CRISPR-Cas9 is a gene editing technology that uses the bacterial immune system to cut DNA at specific locations. It allows researchers to understand, characterize, and control DNA. CRISPR-Cas9 uses an RNA-guided DNA endonuclease enzyme called Cas9 that is directed by guide RNA to cleave target DNA. It has numerous applications including modifying genes in plants and animals, developing disease resistant crops, and potentially curing genetic diseases in humans by precisely editing genes. While revolutionary, it also raises ethical concerns that must be considered and addressed.
Genome editing
genome editing is the emerging branch which includes the specific and precise engineering of genomic regions
Crispr cas: A new tool of genome editing
The document summarizes a presentation on CRISPR cas9, a new genome editing tool. It discusses the history of CRISPR, how CRISPR functions in bacteria, the classification and components of CRISPR systems, and the mechanism of CRISPR cas9. It then covers applications of CRISPR cas9 in genome editing, databases of CRISPR sequences, case studies using the technology, and future directions of CRISPR research.
Crispr cas
This document summarizes Jai Kishan's presentation on gene manipulation techniques. It discusses conventional plant breeding, mutation breeding, transgenic plants, and several precise gene editing techniques like ZFN, TALENs, and CRISPR-Cas9. CRISPR-Cas systems provide adaptive immunity in bacteria and archaea by recognizing foreign DNA. The document then reviews the history and components of the CRISPR-Cas system and its applications in cancer therapy, viral diseases, bacterial infections, and genetic disorders. It also discusses challenges like delivery methods, mosaicism, immune responses, and off-target mutations. Key references on the discovery of CRISPR and its use in agriculture and as a genetic manipulation tool are presented.
Crispr cas 9 presentation
CRISPR Cas-9 is a genome editing technology that uses a bacterial immune system mechanism to allow permanent modification of genes within organisms. It involves clustered regularly interspaced short palindromic repeats (CRISPRs) found in bacterial genomes that play a key role in defense against viruses. The Cas9 protein has six domains including two nuclease domains that cut single-stranded DNA and allow it to be used for genome editing applications like gene silencing, DNA-free editing, and homology-directed repair.
Crispr cas9 scalpels and their application
This document provides information on CRISPR Cas9 genome editing. It discusses the history and discovery of CRISPR dating back to 1987. It describes the key components of the CRISPR Cas9 system including Cas9 proteins, CRISPR RNA, protospacers, and PAM sequences. The mechanisms of how CRISPR Cas9 edits genomes through double strand breaks is explained. Finally, applications of CRISPR Cas9 are summarized, including using it to correct genetic mutations causing diseases in animals and potential applications in humans.
CRISPR-Cas9 system a tool for gene editing presentation
CRISPR Cas9 System for Gene Editing
The document summarizes CRISPR Cas9 gene editing. It discusses the timeline of CRISPR discoveries from 1987-2019. It describes the classification, structure, and mechanism of the CRISPR Cas9 system. Applications discussed include using CRISPR in bacteria, wheat, and to alter muscle mass in humans. Ethical concerns regarding uses in human embryos and potential for non-medical enhancement are also covered.
Crispr cas9
CRISPR, CAS9, Genome Editing, ZFNS, TALENS, Spacer DNA, tracrRNA, crRNA, sgRNA, PAM, CRISPAR-CAS9, CAS GENES, Gene editing, gene silencing, gene knockout, CAS protein, enzyme, nuclease, restriction enzyme, CRISPR Array, Genetic Engineering, Applications of CRISPR, Advantages of CRISPR, LIMITATIONS OF CRISPR, DISADVANTAGES OF CRISPR, BENEFITS OF CRISPR, Future of CRISPR, CRISPR and Cancer, Ethical Issues.
CRISPR-Cas9 mediated genome editing : A comprehensive review with zebrafish a...
CRISPR-Cas9 mediated genome editing : A comprehensive review with zebrafish a...Arkaprava Roychaudhury
This document provides an overview of the CRISPR-Cas9 genome editing system. It discusses the history and mechanism of genome editing, describes the key components and mechanism of CRISPR-Cas9, and outlines its applications in research including in animal models like zebrafish. The document also discusses advantages and disadvantages of CRISPR-Cas9 as well as ethical issues and future prospects of this genome editing technique.Crispr cas9 technology
This document provides an overview of CRISPR-Cas9 technology. It defines CRISPR as DNA sequences in bacteria that contain snippets of viral DNA used to detect and destroy invading viruses. The CRISPR system contains Cas9 proteins that cut DNA at specific locations guided by RNA, and RNA guide molecules. It works by integrating viral DNA into the bacteria's CRISPR loci, which are then transcribed into RNA to guide Cas9 to cleave invading viral DNA. Applications of CRISPR include disease modeling, cancer research, and correcting mutations in human embryos.
Crispr cas9
This document discusses the CRISPR-Cas9 genome editing technique. It begins with an overview of genome editing and provides a brief history. It then focuses on explaining CRISPR-Cas9, including its key components, how it was discovered as a natural bacterial immune system, and how it functions as a genomic tool. The document outlines the general CRISPR-Cas9 protocol and recent advances in the technique. It discusses applications in agriculture and for diseases. It also touches on advantages and limitations, as well as ethical issues. Two case studies are provided that demonstrate using CRISPR-Cas9 to modify genes in rice plants.
Crispr cas 9
This document provides information about CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) including:
1) CRISPR is a genome editing technique that uses the Cas9 enzyme to cut DNA at a targeted location, allowing DNA inserts or modifications.
2) It was originally discovered as a bacterial immune system but is now the most widely used approach for genome engineering due to its simplicity, precision, and versatility.
3) There is an ongoing patent dispute between researchers Jennifer Doudna, Emmanuelle Charpentier, and Feng Zhang over who first demonstrated using CRISPR-Cas9 for genome editing in human cells.
Crispr/cas9 101
This document provides an overview of CRISPR/Cas9 genome editing. It discusses the history and limitations of prior genome engineering techniques like recombinant DNA and zinc finger nucleases. It then explains how CRISPR/Cas9 works as a RNA-guided DNA endonuclease and how this allows it to efficiently and specifically edit genomes. The document outlines several applications of CRISPR/Cas9 like generating knockout animals and cell lines. It also notes some concerns about using the technique for human genome editing.
CRISPR Technology
A simple version of the CRISPR/Cas system, CRISPR/Cas9, has been modified to edit genomes. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added.
Crispr
This document summarizes a seminar given on CRISPR technology. It discusses the history of CRISPR research from its discovery in bacteria in 1987 to its development as a genome editing tool in 2012. Key events and applications are outlined, including the founding of early biotech companies utilizing CRISPR. The core concepts of how CRISPR induces double strand breaks and DNA repair are explained. Recent advances in engineered Cas9 variants and discovery of Cpf1 are also summarized.
CRISPR-Cas systems and applications
i explained about basics of genome engineering and crispr system.
CRISPR will change the world and it is just the beginning, are you ready to meet the future? you think its great and beautiful or.....?
please give your feedback to my email
pooyanaghshbandi@yahoo.com
i am starting to write a critical and fantastic review article about CRISPR, if you are interested to join please contact me.
Crispr handbook 2015
This handbook describes CRISPR/Cas9 genome editing and other research applications for CRISPR technology.
CRISPR-Cas system
CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found within the genomes of prokaryotic organisms such as bacteria and archaea. These sequences are derived from DNA fragments of bacteriophages that have previously infected the prokaryote and are used to detect and destroy DNA from similar phages during subsequent infections. Hence these sequences play a key role in the antiviral defense system of prokaryotes.
Cas9 (CRISPR-associated protein 9) is an enzyme that uses CRISPR sequences as a guide to recognize and cleave specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within organisms.This editing process has a wide variety of applications including basic biological research, development of biotechnology products, and treatment of diseases.
The CRISPR-Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as those present within plasmids and phages that provides a form of acquired immunity. RNA harboring the spacer sequence helps Cas (CRISPR-associated) proteins recognize and cut foreign pathogenic DNA. Other RNA-guided Cas proteins cut foreign RNA. CRISPR are found in approximately 50% of sequenced bacterial genomes and nearly 90% of sequenced archaea.
What's hot (20)
CRISPR-Cas9 system a tool for gene editing presentation
CRISPR-Cas9 system a tool for gene editing presentation
CRISPR-Cas9 mediated genome editing : A comprehensive review with zebrafish a...
CRISPR-Cas9 mediated genome editing : A comprehensive review with zebrafish a...
Viewers also liked
An Introduction to Crispr Genome Editing
In this short presentation, I make a case for doing genome editing vs some of the approaches that have gone before, describe some of the tools available, and the focus on CRISPR-Cas9, what it is, where it's come from and how it works.
the application of CRISPR/Cas9 system in genome editing
CRISPR-cas9 system is a new robust genome editing tool and also this is a really easy to use system.
کاربرد سیستم کریسپر در ویرایش ژنوم.ppt
CRISPR - gene-editing for everyone
Have you considered that protein over-expression or inefficient mRNA knockdown may be masking physiological effects in your assays? Increasingly scientists are moving to endogenous gene-editing to characterise the function of their genes of interest.
Dr Chris Thorne from Cambridge Biotech Horizon Discovery discusses the ground breaking gene-editing technology CRISPR. The simplicity of experimental design has led to rapid adoption of the technology across the scientific community. However, challenges remain.
This Slidedeck focuses specifically on implementing CRISPR experiments, and explore a number of key considerations crucial to maximising chances of targeting success, whether your goal is to generate a knock-out or a knock-in. Chris also takes a look at some of the alternative uses of CRISPR, including sgRNA genome wide synthetic lethality screens.
The slides aim to support those researchers either planning to or already using CRISPR gene-editing in their lab. Horizon Discovery have also recently launched a program aimed specifically at academic cell biologists to promote the adoption of CRISPR by offering FREE CRISPR Reagents for knock-out cell line generation - more information available here. http://www.horizondiscovery.com/what-we-do/discovery-toolbox/genassist-crispr--raav-genome-editing-tools
Reprogramming the genome with CRISPR
This document discusses the use of CRISPR gene editing technology. It provides examples of how CRISPR has been used to cure diseases in animals and potentially humans, create customized cancer models and modify animal organs. It also describes how CRISPR can be applied in agriculture to develop drought-resistant and pest-resistant crops, as well as in industrial biotechnology settings. The document then explains how gene drives work to alter genes and spread them through populations using CRISPR. It calls for responsible development of this technology through community guidance, transparency and democratic decision making.
Increase efficiency of genome editing with the Alt-R™ CRISPR-Cas9 System: Des...
Increase efficiency of genome editing with the Alt-R™ CRISPR-Cas9 System: Des...Integrated DNA Technologies
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. In a recent webinar, "New RNA Tools for Optimized CRISPR/Cas 9 Genome Editing", we presented how we developed the Alt-R™ CRISPR-Cas9 System for genome editing. Here, we take a look at designing your target sequences and ordering them as Alt-R CRISPR crRNA. We review the other components of the system and walk through the experimental process step by step, from design to evaluation of editing potency. We also discuss challenges and potential pitfalls and provide tips and guidance towards successful genome editing experiments. Learn more: http://www.idtdna.com/crisprApplications of crispr cas system
CRISPR-Cas is a genome editing technique derived from bacterial immune systems that allows for precise genomic modifications. The document discusses applications of CRISPR-Cas in plants, animals, and bacteria, including developing pest and disease resistant crops and livestock, modifying stem cells and embryos, targeting antibiotic resistant bacteria, and controlling gene expression.
Gene Editing - Challenges and Future of CRISPR in Clinical Development
In this presentation, Medpace experts discuss the unique opportunity of evolving gene editing technology as it applies to human diseases
Genome Editing Comes Of Age
Recent breakthroughs in genome editing technology have led to a rapid adoption that parallels that seen with RNAi. And like RNAi, these methods are taking the scientific world by storm, with high profile publications in fields as diverse as HIV treatment, stem cell therapy, food crop modification and drug development to name but a few.
Critically, the endogenous modification of genes enables the study of their function in a physiological context. It also overcomes some of the artefacts that can result from established techniques such as transgenesis and RNAi, which have mislead researchers with false positives or negatives. Until recently however genome editing required considerable technical expertise, and consequently was a relatively niche pursuit.
In this talk we will look at how the latest developments in genome editing tools have changed this, with improvements in both ease-of-use and targeting efficiency, as well as a concomitant reduction in costs opening up these approaches to the wider scientific community.
Rapid adoption of the CRISPR/Cas9 system has for example led to a long list of organisms and tissues in which genetic changes have been made with high efficiency. Other technologies such as recombinant adeno-associated virus (rAAV) offer further precision, stimulating the cell’s high-fidelity DNA repair pathways to insert exogenous sequence with unrivalled specificity. Targeting efficiency can be improved still further by using the technologies in combination – genome cutting induced by CRISPR can significantly enhance homologous recombination mediated by rAAV.
Despite these rapid advances, some pitfalls remain, and so we’ll discuss some of the key considerations for avoiding these, ranging from simply picking the right tool for the job to designing an experiment that maximises chances of success.
Finally we’ll look at how genome editing is being applied to both basic and translational research, and in both a gene-specific and genome wide manner. For the study of disease associated genes and mutations scientists can now complement wide panels of tumour cells with genetically defined isogenic cell pairs identical in all but precise modifications in their gene of interest. The ease-of-design and efficiency of the CRISPR system is also being exploited for genome wide synthetic lethality screens, facilitating rapid drug target identification with significantly reduced risk of false negatives and off-target false positives. And again, further synergies are achieved when these approaches are combined to look for potential synthetic lethal targets in specific genomic contexts.
Lessons learned from high throughput CRISPR targeting in human cell lines
In just a short period of time CRISPR-Cas9 technology has revolutionized the field of genome editing, and taken the scientific community by storm. Already our understanding of how best to apply this technology has advanced significantly and almost every week new publications appear showcasing its application in basic and translational research.
While CRISPR-Cas9 is applicable across many different cell types, we have found it particularly suited for genome editing in near-haploid human cell lines. This has allowed us to establish a robust pipeline for the inactivation of non-essential genes at unprecedented scale and efficiency.
We have now knocked out over 1500 human genes and have generated a resource that is, to the best of our knowledge, the largest collection of human knockout cell lines available, covering comprehensive subsets of genes clustered by biological pathway (e.g. the autophagy pathway, the JAK/STAT pathway) or by phylogenetic relationship (e.g. kinases, bromodomain-containing proteins).
In this talk we will discuss how, through more than 1500 genome editing experiments, we have started to unravel some of the general principles governing the use of CRISPR-Cas9 in mammalian cells. For example, we have analyzed the impact of variation in the guide RNA sequence on Cas9 cleavage efficiency and characterized the mutational signature arising from CRISPR-Cas9 cleavage.
We will also highlight (with examples) how these learnings are now being applied to introduce other genomic modifications in a high throughput manner, including chromosomal deletions, translocations, point mutations and endogenous gene tags.
Genome editing tools article
Genome editing tools form the basis for personalized medicine, especially for therapies requiring change in genome. Currently there are four contenders to this – Meganucleases, ZNF Nucleases, TALENs and CRISPRs. Although, the technologies are many, there are very few commercial providers of this technology. This is attributed to the fact that select few possess the intellectual property rights of turning these technologies to valid form of therapy; for example, ZFN patent with Sangamo BioSciences and TALENs with Cellectis, Transposagen and Life Technologies.
Synthetic biology
Genome editing methods such as ZFNs, TALENs, and CRISPR/Cas9 use engineered nucleases to create targeted double-stranded breaks in DNA which are then repaired through endogenous cellular processes. These nucleases can be used to modify genomes through techniques like gene knockout, targeted mutation insertion/deletion/correction, and studying gene function. CRISPR/Cas9 uses a guide RNA and Cas9 nuclease to target specific DNA sequences for editing. The four main steps for CRISPR are: 1) selecting target sequences near a PAM site, 2) designing and cloning gRNA, 3) delivering Cas9 and gRNA into cells, and 4) DNA repair after cleavage results in gene modification
Global CRISPR Market | Forecast 2015 - 2021
Global CRISPR Market (Genome Editing) is expected to reach $268 million by 2021 Geographical Analysis Country (United States, Canada, India, China, Japan, United Kingdom), Company Profiles, Share, Trends, Analysis, Opportunities, Segmentation and Forecast 2015 – 2021
Crispr
This document provides an overview of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and its role as an adaptive immune system in prokaryotes. It describes the components and function of the CRISPR-Cas system, including how it provides immunity against viruses and plasmids. Applications of CRISPR technology discussed include phage resistance in bacteria, gene regulation, and bacterial strain typing. Potential future uses involve harnessing CRISPR biology for applications like transcriptional control.
CRISPR cas, a potential tool for targeted genome modification in crops.
Clustered regularly interspaced short pallindromic repeats(CRISPR) is the newest strategy of targeted genome modification.
Rewriting the Genome Using CRISPR and Synthetic Biology
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. Additionally, the simple gene targeting mechanism of CRISPR technology has been modified and adapted to other applications that include gene regulation, detection of intercellular trafficking, and pathogen detection. With a wealth of methods for introducing Cas9 and gRNAs into cells, it can be challenging to decide where to start. In this presentation, Dr Adam Clore describes the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in designing, testing, and executing a variety of CRISPR-mediated experiments. For more informaton, visit: http://www.idtdna.com/crispr
CRISPR: what it is, and why it is having a profound impact on human health
This document summarizes a webinar on CRISPR that included presentations from experts in gene editing and bioinformatics. The webinar provided an overview of CRISPR and how it works using the Cas9 enzyme and guide RNA to make precise cuts in DNA. It discussed how CRISPR is being used for gene knockout studies, clinical trials to treat diseases like cystic fibrosis and cancer, and the challenges of predicting off-target effects. The webinar highlighted both the promise and challenges of CRISPR for accelerating scientific discovery and developing new gene therapies.
New RNA tools for optimized CRISPR/Cas9 genome editing
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying the genomes of organisms ranging from E. coli to humans. In this presentation, we discuss various methods for generating the crRNA and tracrRNA components that are required for guiding the Cas9 endonuclease to genomic targets. You will also learn how to optimize a new 2-part CRISPR RNA system from IDT that offers multiple benefits over other technologies.
CRISPR Is On The Move: Genome Editing From Rice To Wheat
Presented at Plant Genomics and Gene Editing Congress: Europe. For more information visit: www.global-engage.com
Success in rice is not equalled in wheat: CRISPR/Cas9 efficiency is around 5% and requires a time-consuming downstream identification of the genome edited plants.
Transposagen Q3 2012 Overview
Transposagen Biopharmaceutical’s 2012 current corporate overview of our translational RandD + preclinical services.
Harnessing Beneficial Microbial Communities: Opportunities And Challenges For...
Presented at Global Biocontrol Biostimulants Congress: USA. For more information visit: www.global-engage.com
Viewers also liked (20)
the application of CRISPR/Cas9 system in genome editing
the application of CRISPR/Cas9 system in genome editing
Increase efficiency of genome editing with the Alt-R™ CRISPR-Cas9 System: Des...
Increase efficiency of genome editing with the Alt-R™ CRISPR-Cas9 System: Des...
Gene Editing - Challenges and Future of CRISPR in Clinical Development
Gene Editing - Challenges and Future of CRISPR in Clinical Development
Lessons learned from high throughput CRISPR targeting in human cell lines
Lessons learned from high throughput CRISPR targeting in human cell lines
CRISPR cas, a potential tool for targeted genome modification in crops.
CRISPR cas, a potential tool for targeted genome modification in crops.
Rewriting the Genome Using CRISPR and Synthetic Biology
Rewriting the Genome Using CRISPR and Synthetic Biology
CRISPR: what it is, and why it is having a profound impact on human health
CRISPR: what it is, and why it is having a profound impact on human health
New RNA tools for optimized CRISPR/Cas9 genome editing
New RNA tools for optimized CRISPR/Cas9 genome editing
CRISPR Is On The Move: Genome Editing From Rice To Wheat
CRISPR Is On The Move: Genome Editing From Rice To Wheat
Harnessing Beneficial Microbial Communities: Opportunities And Challenges For...
Harnessing Beneficial Microbial Communities: Opportunities And Challenges For...
Similar to CRISPR technology -A revolutionary discovery
Genome Editing with CRISPR-Cas9
CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.
CRISPR
Gene editing using CRISPR was originally discovered as a bacterial immune system that provides resistance to viruses. CRISPR uses specialized DNA sequences and associated Cas proteins to create targeted double-strand breaks in DNA, allowing modification of genomes. The technique has rapidly advanced due to its simplicity and versatility compared to prior tools. CRISPR holds promise for treating genetic diseases, transplantation, biotechnology, disease models, and more. It has become a widely used research tool with many companies and publications emerging around its applications.
CRISPR CAS
CRISPR/Cas9 is a prokaryotic immune system that confers resistance to foreign genetic elements. It has been modified to edit genomes by delivering the Cas9 nuclease complexed with a guide RNA to cut DNA at a desired location. The system proceeds in three stages: adaptation incorporates new spacers into the CRISPR array, crRNA biogenesis processes the pre-crRNA, and target interference uses the crRNA-Cas9 complex to degrade invading DNA. CRISPR/Cas9 provides a simple and efficient tool for gene editing but also raises ethical concerns regarding its applications.
Crispr cas in antimicrobial research
The document summarizes a seminar on the role of CRISPR-Cas9 in antimicrobial resistance. It first describes the components and mechanism of the CRISPR-Cas9 system, including how it was discovered and can be used for gene editing. It then discusses how CRISPR-Cas9 can be programmed to target and cleave DNA sequences, including antibiotic resistance genes. Specifically, a CRISPR-Cas9 system can be built into a bacteriophage to target and disrupt bacteria carrying resistance genes. Studies have shown this approach can successfully target genes conferring resistance to antibiotics like carbapenem, kanamycin, ceftazidime, and methicillin.
CRISPR Cas 9 TECHNOLOGY
CRISPR Cas9 is a genome editing technology that allows genetic material to be added, removed, or altered from a genome. It originated as a bacterial immune system but can now be directed to make precise edits to DNA. The technology has wide applications for gene therapy, agriculture, research, and more, but also raises ethical concerns if misused. CRISPR offers promising possibilities but also challenges that must be addressed regarding safety, accuracy, and societal effects.
CRISPR/CAS9- THE GENE EDITING TOOL
The document provides an overview of the CRISPR/Cas9 gene editing technology. It discusses the history and components of the CRISPR system, how it works, applications in various fields like microbiology, biomedicine, agriculture, and therapeutics. Recent advances expand its use for transcriptional regulation, epigenetic editing, and live imaging. While powerful, it faces challenges like off-target effects that require further research to optimize its safe and ethical application.
Crispr cas9
This document summarizes information about the CRISPR Cas9 genome editing tool. It discusses how CRISPR Cas9 uses guide RNA and the Cas9 enzyme to create targeted double-strand breaks in DNA, allowing genes to be knocked out or altered. The document outlines the history and mechanism of CRISPR Cas9, compares it to other genome editing tools, discusses its applications in plant breeding including reducing off-target effects, and provides an example of using it to create parthenocarpic tomato plants.
The relative ease of use and reproducibility of
CRISPR-Cas is a powerful tool for studying virus-host interactions and identifying new antiviral targets. It allows for easy and reproducible probing of host factors involved in viral pathogenesis. CRISPR-Cas systems use CRISPR RNA and Cas proteins to target and cleave viral DNA. Pooled CRISPR screens can identify host dependency factors for viruses. CRISPRa and CRISPRi techniques can also be used to study the effects of overexpressing or suppressing host genes on viral replication. Overall, CRISPR-Cas provides new opportunities to discover antiviral drug targets by manipulating host genes involved in viral infection cycles.
Crisper cas system
The document summarizes the CRISPR-Cas system. It discusses how CRISPR-Cas provides adaptive immunity in bacteria and archaea by integrating short DNA sequences from invading viruses and plasmids between repeated DNA sequences in the host's CRISPR array. It then describes the key components of the CRISPR-Cas system including the CRISPR array, cas genes, and how the system acquires spacers and functions through different steps of biogenesis and interference to target invading genetic elements. Finally, it discusses some potential applications and limitations of using the CRISPR-Cas system for genome editing in areas like genetic diseases, cancer, and HIV.
genome editing technique CRISPR-Cas9 - Copy.pptx
CRISPR-Cas9 is a gene editing technique that utilizes the Cas9 enzyme to cut DNA at specific locations guided by CRISPR RNA. It allows scientists to precisely modify genes and has applications in medicine, agriculture, and scientific research. Some examples include developing disease-resistant crops and mosquitoes, growing human organs in pigs, and potentially curing genetic diseases. While promising, CRISPR also faces ethical concerns regarding safety, unintended effects, germline editing, and unequal access to treatment. Overall, CRISPR is a revolutionary new biotechnology but more research is still needed to fully realize its benefits and address ethical implications.
CRISPR-Cas9
This slide will help you understand the basics of CRISPR-Cas9, Mechanism, Application, Advantages, and Disadvantages of CRISPR-Cas9, Future Concerns, Future Possibilities.
Crispr
a new method in gene editing first discovered in bacteria but nowadays is used in knocking out or modifying gene changes .
CRISPR CAS9 technique
This document discusses the CRISPR-Cas9 genome editing technique. It begins with an introduction to CRISPR as an adaptive immune system in bacteria. The CRISPR mechanism involves acquiring DNA from invading viruses and using CRISPR RNA and Cas9 proteins to cut matching viral DNA. Scientists now use the Cas9 nuclease guided by a synthetic single guide RNA to make targeted cuts in DNA for genetic engineering. Some applications include modifying crop plants and research in mice embryos. However, using CRISPR in human embryos raises ethical concerns about germline editing and unintended consequences.
CRISPR Cas9 technology.pptx
This document provides an overview of CRISPR-Cas9 technology. It describes how CRISPR sequences in bacteria contain snippets of DNA from viruses that have attacked the bacterium. These sequences are used by the bacterium to detect and destroy DNA from further viral attacks. The document outlines the mechanism of how the Cas9 protein works with a guide RNA to target and cut specific areas of DNA. Applications of CRISPR-Cas9 technology include gene editing in plants, animals and humans to treat genetic diseases, develop crops with desired traits, and further biomedical research.
Genome editing with engineered nucleases
Genome editing uses engineered nucleases to insert, replace or remove DNA from the genome. These nucleases create targeted double-strand breaks which are repaired through natural DNA repair processes, allowing for changes to the genome sequence. Three main engineered nuclease systems for genome editing are ZFNs, TALENs, and CRISPR-Cas9. CRISPR uses a guide RNA and Cas9 nuclease to make precise cuts at targeted DNA sequences for editing. It has advantages over ZFNs and TALENs in being cheaper, easier to design, and more efficient. Genome editing holds promise for applications in crops, medicine, and research.
CRISPRCas_Biotechnology_GE_AmanNanavaty.pdf
Through this presentation, you can understand the basics of CRISPR-Cas systems and their importance in various fields of Biotech. The molecular mechanisms involved in CRISPR-Cas are clearly elucidated with appropriate diagrams. The applications of CRISPR-Cas Genetic Engineering in fields such as BioPharma, Plant Biotech and Bioproduction are listed out as well.
CRISPR_cas9_tech.pptx
CRISPR cas9 technology is a genome editing technique which won the noble prize in 2021.
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats.
Genetic Engineering, Gene editing, Advantages of CRISPR, Limitations of CRISPR and Applications of CRISPR,
The Genome editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...
Role of CRISPR/Cas9 in plant pathology
Production of disease resistance cultivars by editing the genome which is responsible for susceptibility factor for fungal and bacterial diseases.
By editing the genome which governs host pathogen interaction we can obtain incompatible interaction between host pathogen.
To improve the efficacy of bio control agents.
By editing the genome responsible for virus multiplication and virulence we can obtain virus free resistance cultivars.
The Genome-editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...
Role of CRISPR/Cas9 in plant pathology
Production of disease resistance cultivars by editing the genome which is responsible for susceptibility factor for fungal and bacterial diseases.
By editing the genome which governs host pathogen interaction we can obtain incompatible interaction between host pathogen.
To improve the efficacy of bio control agents.
By editing the genome responsible for virus multiplication and virulence we can obtain virus free resistance cultivars.
Crispr cas
This document provides an overview of CRISPR/Cas9 genome editing. It discusses how CRISPR/Cas9 enables precise modification of DNA sequences, outlines the timeline of key discoveries in CRISPR research, and describes the molecular mechanism and potential applications of this technology, including in microbial research, crop improvement, and human gene therapy. It also notes some limitations of the CRISPR/Cas9 system and concludes by emphasizing the opportunities it provides to advance research and address challenges like food security.
Similar to CRISPR technology -A revolutionary discovery (20)
The Genome editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...
The Genome editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...
The Genome-editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...
The Genome-editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...
Recently uploaded
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
ESR spectroscopy in liquid food and beverages.pptx
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
SAR of Medicinal Chemistry 1st by dk.pdf
In this presentation include the prototype drug SAR on thus or with their examples .
Syllabus of Second Year B. Pharmacy
2019 PATTERN.
Thornton ESPP slides UK WW Network 4_6_24.pdf
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
bordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless ReproducibilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Recently uploaded (20)
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
ESR spectroscopy in liquid food and beverages.pptx
ESR spectroscopy in liquid food and beverages.pptx
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt
mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Equivariant neural networks and representation theory
Equivariant neural networks and representation theory
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
Basics of crystallography, crystal systems, classes and different forms
Basics of crystallography, crystal systems, classes and different forms
bordetella pertussis.................................ppt
bordetella pertussis.................................ppt
Authoring a personal GPT for your research and practice: How we created the Q...
Authoring a personal GPT for your research and practice: How we created the Q...
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless Reproducibility
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
The debris of the ‘last major merger’ is dynamically young
The debris of the ‘last major merger’ is dynamically young
CRISPR technology -A revolutionary discovery
- 1. CRISPR TECHNOLOGY Guided by: Mr.Baji.K Presented by: P.Navaneeth Krishna Menon
- 2. INTRODUCTION • What is CRISPR Technology? • Clustered Regularly Interspaced Short Palindromic Repeats. • CRISPR/Cas system is used for genome editing. 2
- 3. CRISPR/Cas System • CRISPRs are segments of prokaryotic DNA containing short repetitions of base sequences. • CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as phages. • Much easier to design compared to its predecessors zinc finger nucleases and TALENs. 3
- 4. • Spacers are fragments of DNA gathered from the viruses that had tried to attack the cell. • CRISPR loci range from 24 to 48 bp. • CRISPR-associated(cas) genes are attached to the repeater-spacer array. 4
- 5. Action of CRISPR The CRISPR immune system works to protect bacteria from repeated viral attack via three basic steps: (1) Adaptation (2) Production of CRISPR RNA (3) Targeting 5
- 6. 6
- 7. APPLICATIONS In industry: CRISPR system are advantageous for industrial processes that utilize bacterial cultures. CRISPR-based immunity can be employed to make these cultures more resistant to viral attack, which would otherwise impede productivity. Eg: Streptococcus thermophilus, which is used to make yogurts and cheeses. 7
- 9. 9
- 10. IIn Medicine: Treatment of genetic diseases. To make more specific antibiotics that target only disease-causing bacterial strains while sparing beneficial bacteria. Researches are being carried out to make WBCs resistant to HIV infection. 10
- 11. CONCLUSION Jennifer Doudna ,Emmanuelle Charpentier and Feng Zhang 11
- 12. 12
- 13. 13
- 14. REFERENCE • http://sitn.hms.harvard.edu/flash/2014/crispr-genetic- engineering-technique/ • Horvath P, Barrangou R (2010). "CRISPR/Cas, the immune system of bacteria and archaea". Science 327 (5962): 167–70 • https://www.addgene.org/crispr/guide/ • Sawyer E (9 February 2013). "Editing Genomes with the Bacterial Immune System".Scitable (blog). Nature Publishing Group. 14
- 15. 15