1) Nanotechnology involves studying and manipulating matter at the nanoscale, and nanoparticles are particulate substances that are less than 100 nm in at least one dimension. 2) The COVID-19 pandemic has spread to over 197 countries, infecting over 53.9 million people and killing over 1.31 million as of now. 3) RT-PCR is commonly used to detect SARS-CoV-2 but requires laboratory processing and skilled personnel, limiting its effectiveness for outbreak control.
Introduction
Definition
History
Advantages of nanobiotechnology
Applications of nanobiotechnology
Drawback of nanobiotechnology
New features in the nanobiotechnology
Conclusion
References
Application of Nanotechnology in Natural ProductsMona Ismail
Nanoscience is the manipulation of materials at atomic, molecular and macromolecular scales, where properties differ significantly from those at a larger scale.
The word "Nano" is derived from the Greek word for “Dwarf”. It means a billionth. A nanometer is a billionth of a meter.
Introduction
Definition
History
Advantages of nanobiotechnology
Applications of nanobiotechnology
Drawback of nanobiotechnology
New features in the nanobiotechnology
Conclusion
References
Application of Nanotechnology in Natural ProductsMona Ismail
Nanoscience is the manipulation of materials at atomic, molecular and macromolecular scales, where properties differ significantly from those at a larger scale.
The word "Nano" is derived from the Greek word for “Dwarf”. It means a billionth. A nanometer is a billionth of a meter.
Applications of nanobiotechnology by kk sahuKAUSHAL SAHU
INTRODUCTION
DEFINITION
HISTORY
NANOSCALE
NANOPARTICLES
NANOBIOTECHNOLOGY
NANOTOOLS
APPLICATIONS
RESEARCH
CONCLUSION
REFRENCES
Nanotechnology is the design, characterization and application of structures, devices and systems by controlling shape and size at the nanometer scale!” defines the Royal Academy of Engineering in London in 2004 .
Concepts that are enhanced through nanobiology include: nanodevices, nanoparticles, and nanoscale phenomena that occurs within the discipline of nanotechnology.
My Guest Lecture at "TROPACON 2011", 5th National Conference of Indian Academy of Tropical Parasitology, 11th-13th November, 2011 at Department of Microbiology, Government Medical College, Nagpur, Maharashtra, India
An overview of the Nanotechnology through the aspects of Forensic Science ; its types , importance in various crimes, fields as a evidence collector .
It will help you to study the initials of this field that could be used in various crime investigations.
I hope you find it interesting and it helps you in whatever purpose you are studying this for. Do contact me for any further presentations that I could help you up with.
Applications of nanobiotechnology by kk sahuKAUSHAL SAHU
INTRODUCTION
DEFINITION
HISTORY
NANOSCALE
NANOPARTICLES
NANOBIOTECHNOLOGY
NANOTOOLS
APPLICATIONS
RESEARCH
CONCLUSION
REFRENCES
Nanotechnology is the design, characterization and application of structures, devices and systems by controlling shape and size at the nanometer scale!” defines the Royal Academy of Engineering in London in 2004 .
Concepts that are enhanced through nanobiology include: nanodevices, nanoparticles, and nanoscale phenomena that occurs within the discipline of nanotechnology.
My Guest Lecture at "TROPACON 2011", 5th National Conference of Indian Academy of Tropical Parasitology, 11th-13th November, 2011 at Department of Microbiology, Government Medical College, Nagpur, Maharashtra, India
An overview of the Nanotechnology through the aspects of Forensic Science ; its types , importance in various crimes, fields as a evidence collector .
It will help you to study the initials of this field that could be used in various crime investigations.
I hope you find it interesting and it helps you in whatever purpose you are studying this for. Do contact me for any further presentations that I could help you up with.
Leveraging nanotechnology and biology for medical diagnostics. Including novel techniques such as immuno-PCR and using phages as reporters, as well as using Izon's qNano to detect DNA hybridization and potential uses in point-of-care applications.
A part of nanotechnology. Nanosensors is very hot topic for research. As nanosensor has immense applications in the fields like medical, analysis, research etc. Nanosensor recude the cost and also the time require for analysis.
Nanobiotechnology, bionanotechnology, and nanobiology are terms that refer to the intersection of nanotechnology and biology. Given that the subject is one that has only emerged very recently, bionanotechnology and nanobiotechnology serve as blanket terms for various related technologies.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Similar to Covid-19 testing using nanoparticles (20)
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
PRESENTATION ABOUT PRINCIPLE OF COSMATIC EVALUATION
Covid-19 testing using nanoparticles
1.
2. • Nanotechnology is a known field of research and was
presented by Nobel laureate Richard P. Feynman during
his well famous 1959 lecture
‘‘There’s Plenty of Room at the Bottom” (Feynman, 1960).
• Nanoparticles (NPs) are wide class of materials that include
particulate substances, which have one dimension less than
100 nm at least. ( overall shape these materials can be 0D,
1D, 2D or 3D ).
• Importance: size can influence the physiochemical
properties of a substance e.g. the optical properties.
• A 20- nm gold (Au), platinum (Pt), silver (Ag), and
palladium (Pd) NPs have characteristic wine red color,
yellowish gray, black and dark black colors, respectively.
3. • The outbreak of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2, aka COVID-19) has emerged very
rapidly and has already invaded more than 197 countries worldwide, with more than 53.9M cases and ∼1.31 M deaths
till date and increasing , as reported by the WHO.
• About 97.5% of patients develop symptoms of COVID-19 within 11.5 days of exposure, causing late diagnosis and a
high infection rate.
• Coronaviruses (CoVs) : RNA, enveloped viruses grouped into the α-CoVs, β-CoVs,
γ-CoVs, and δ-CoVs genera. β-CoVs infect mammals, with SARS-CoV-1 and
MERS-CoV as the known species able to infect humans until last year.
• Recently, SARS-CoV-2 emerged as a new species able to infect humans and
currently causing the COVID-19 pandemic
• The molecular tests used so far to confirm COVID-19 are considered the gold
standards for SARS-CoV-2 testing. Nevertheless, they require a swab sample and
a time consuming laboratory procedure.
• Shipping of samples and overload of laboratory facilities.
• Delay of many days until the test results are available.
• Local community transmission.
4.
5. RT-PCR is recognized as a high quality and consistently employed tool to analyze and quantify the different
RNA’s in the labs and clinical diagnostics due to elevated sensitiveness in RNA amplification.
Even though, RT-PCR assisted techniques have been extensively applied in COVID-19 detection, their
applicability in precise detection of virus and outbreak control is relentlessly hindered due to time taking processes
and man-power requirement. (Skilled personnel's).
The pure quantification of nucleic acid extracts from the complexive specimens are the main requirement for
potential RT-PCR analysis [6].
The lower efficiency of the extraction may result in the unfortunate indications through the amplification process
and consequently appears in the wrong diagnosis.
Conventional techniques for nucleic acid binding consist many steps which are lengthy, time taking and
susceptible to impurities.
In the supervision and controlling of impulsive epidemics like COVID-19, these conventional techniques may
consume heavy man-power, however, lacking efficient detection with a higher risk of cross-infections.
6. Magnetic nanoparticles (MNP’s) assisted extraction techniques are convenient, simple and companionable
with automotive processes.
The surface functionalized MNP’s adsorbs the nucleic acid from the lysis solution and are quickly isolated
from most of the contaminations with the help of external magnetic field.
Nucleic acid can be further isolated from the functionalized surface of MNP’s by desorption process in
eluent.
Still this consists of several steps, which is insufficient for practical detection.
A more simple and modernize MNP’s assisted RNA-extraction protocol where MNP’s of zinc ferrite (ZNF)
were fabricated by the cost efficient sol-gel auto-combustion route and subsequently, its surface was
functionalized with carboxyl containing polymers (CPoly).
Zinc ferrite : high chemical stability, soft magnetic behaviour (Coercivity), easy preparation, biocompatible
nature.
Powerful interface among nucleic acids and carboxyl groups, the surface functionalized MNP’s facilitate
speedy and potential adsorption of viral RNA’s
7. 1.)Nitrates of metal ions (Zn2+, Fe3+) + citric acid
(C6H8O7) dissolved in DD water. Continuously
stirred for half an hour
2.) pH adjusted to the 7 by adding liquid ammonia.
3.) Continuously stirred , heated at 80° C until it
gets converted into gel.
5.) After gel formed, heat was raised to 120°C
which resulted in the auto combustion of the gel to
form a fluffy loose powder.
6.) Powder was grinded , sintered at 600°C for 4 h
to acquire good crystallinity.
7.) Sintered ZNF NP’s then used to prepare silica-
coated ZNF NP’s (ZNF@Si).
8.) Continuously stirred for 3 h at 27°C to form as
silica layer on the core of ZNF NP’s. The silica
modified ZNF NP’s then accumulated using
magnet.
8. 9.) APTES was added drop-by-drop in the
solution, containing the mixture of ZNF@Si
and isopropanol and ultrasonicated for 4 h
to modify the surface of ZNF@Si with NH2.
10.) NH2 modified ZNF@Si
(ZNF@Si@NH2) then accumulated using
magnet.
11.) ZNF@Si@NH2 was inserted into the
DMSO solution and subsequently mixed
with 3 g of carboxyl-modified PVA (CPoly)
and then ultrasonicated for 15 min.
12.) CPoly functionalized ZNF@Si@NH2
(ZNF@Si@NH2 @CPoly) then accumulated
using magnet.
More about APTES:
https://www.sigmaaldrich.com/catalog/pro
duct/aldrich/440140?lang=en®ion=IN
9.
10. The sensors are composed of different gold nanoparticles linked to organic ligands, creating a
diverse sensing layer that can swell or shrink upon exposure to volatile organic compounds
(VOCs), causing changes in the electric resistance.
In these layers, the inorganic nanomaterial's are responsible for the electrical conductivity,
with the organic film element providing sites for the adsorption of VOCs.
When exposed, VOCs diffuse into the sensing layer or fall on the sensing surface and react
with the organic segment or the functional groups capping the inorganic nanomaterial's.
The outcome of the interactions causes a volume change (swelling/shrinkage) in the
nanomaterial film.
As a result, the contacts among the inorganic nanomaterial block the change (higher/lower)
with an increase/decrease of conductivity.
The nanomaterial layer exposure to VOCs causes a swift charge transfer to/from the inorganic
nanomaterial, producing variations in the measured conductivity
Different sensors can be used, due to the chemical diversity of the functional group(s)
capping the nanoparticle
11. Figure (a) Example of breath
collection with the developed hand-
held breathalyzer system from a
patient in Wuhan, China.
Figure(b) Representative response of
a sensor to three different breath
samples. The normalized response
of sensor 7 of the breathalyzer
system to three different samples:
patient A, COVID-19, first sample
while infected; patient A, second
sample after determined as
recovered; and a healthy control. The
x-axis represents the cycle
measurement; each unit is one cycle
of the sensor. The infected sample
had a positive change response,
while the recovered and control
showed negative charges
12. REFRENCES:
• MATERIALS RESEARCH INNOVATIONS
https://doi.org/10.1080/14328917.2020.1769350
Multifunctional nano-magnetic particles assisted viral RNA-extraction
protocol for potential detection of COVID-19 Sandeep B. Somvanshia ,
Prashant B. Kharata,b, Tukaram S. Sarafa , Saurabh B. Somwanshic ,
Sumit B. Shejulc and Kamalakar M. Jadhav.
ARTICLE HISTORY Received 28 April 2020 Accepted 11 May 2020.
• Multiplexed Nanomaterial-Based Sensor Array for Detection of
COVID-19 in Exhaled Breath Benjie Shan,△ Yoav Y. Broza,△ Wenjuan
Li,△ Yong Wang, Sihan Wu, Zhengzheng Liu, Jiong Wang, Shuyu
Gui, Lin Wang, Zhihong Zhang, Wei Liu, Shoubing Zhou, Wei Jin,
Qianyu Zhang, Dandan Hu, Lin Lin, Qiujun Zhang, Wenyu Li,
Jinquan Wang, Hu Liu,* Yueyin Pan,* and Hossam Haick*