Comparative evaluation of Physical and Chemical mutagens
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This document presents two case studies on using physical and chemical mutagens to induce variability in gladiolus species. In the first case study, gladiolus corms were treated with gamma rays and diethyl sulphonate (DES) at different doses and concentrations. DES up to 0.3% produced favorable variations and abnormalities while gamma rays did not produce any desirable variations. The second case study treated two gladiolus genotypes with ethyl methane sulphonate (EMS) and gamma rays. Various growth and flowering parameters were recorded and analyzed. The results showed that lower doses of chemical mutagens like EMS 0.25% may be more effective in increasing variability compared to higher doses or physical mutagens like gamma rays
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This document provides an overview of quantitative trait locus (QTL) mapping. It discusses what a QTL is, the types of mapping populations used in QTL mapping like recombinant inbred lines (RILs) and near isogenic lines (NILs), and statistical methods for QTL mapping including single marker analysis, interval mapping, and composite interval mapping. The document also covers factors that affect the power of QTL mapping and limitations of QTL mapping like low resolution and small effects of individual QTLs.
Journal of Ethnobotany | Applications of DNA barcoding and future directions ...
DNA barcoding help to recognize the plant based on short, gene sequences in a rapid, accurate, and cost effective manner. Current focus is on the investigation of phytomedicinals and herbal product integrity and authenticity through DNA barcoding with the goal of protecting consumers from potential health risks associated with product substitution and contamination. Recent reports reveal that DNA barcoding can be used for the assignment of unknown specimens to a taxonomic group, authentic identification of phytomedicinals, and in plant biodiversity conservation. Research indicates that there is no single universal barcode candidate for identification of all plant groups. Hence, comparative analysis of plant barcode loci is essential for choosing a best candidate for authenticating particular medicinal plant genus/families. Currently, both chloroplast/nuclear regions are used as universal barcodes for the authentication of phytomedicinals. A recent advance in genomics has further enhanced the progress in DNA barcoding of plants by the introduction of high-throughput techniques like next generation sequencing, which has paved the way for complete plastome sequencing that is now termed as super-barcodes. Hence, current focus is on the investigation of phytomedicines and herbal product integrity and authenticity through DNA barcoding with the goal of protecting consumers from potential health risks associated with product substitution and contamination. These approaches could improve the traditional ethnobotanical and scientific knowledge of phytomedicines and their safe use.
Hematological Parameters of three Strains of Local Cocks in Northern Nigeria
The study was conducted to determine the hematological parameters of three strains of the Nigerian indigenous cocks. A total of 15 sexually matured (14-18 month of age) breeders cocks comprising (5 normal feathered, 5 frizzled feathered and 5 naked neck) were used for the experiment. The study was conducted from October to December 2016 at the Teaching and Research Farm University of Maiduguri. Blood samples were collected from 9 breeder’s cocks which were randomly selected 3 per genotype and used for hematological parameters examination. Hematological examination such as Packed Cell Volume (PCV), Red Blood Cell ( RBC) , Haemoglobin (Hb), White Blood Cell (WBC), Mean Corpuscular Haemoglobin concentration ( MCHC), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Volume ( MCV) showed significant (P<0.05)> 0.05) different between normal feathered and frizzle feathered but there is significant difference ( P< 0.05) with naked necked cock. Neutrophil ( N) showed significant (P<0.05)>0.05) difference between normal feathered and naked neck feathered , fizzle feathered and necked neck respectively but showed significant (P<0.05) difference between fizzle feathered and normal feathered respectively for M and E. the study concluded that variation in the heamatoloical parameters between three strains of local chicken in Nigeria is due to difference in their genetic makeup.
Damon Little - Opening Plenary
The document discusses using DNA barcoding to identify plant species in 146 commercial tea products. 90% of products yielded DNA barcodes for rbcL or matK genes using a standard protocol. However, matching the DNA identifications to listed ingredients was limited by incomplete reference databases, shared barcodes among species, and lack of standard plant names. About 1/3 of herbal teas contained unlisted plant species according to DNA analysis, demonstrating potential issues with ingredient labeling. The study highlights both the promise and challenges of using DNA barcoding to verify plant ingredients in commercial products.
Crimson publishers-5-MethylcytosineDNA Methylation Patterns among Gut Predomi...
Crimson publishers-5-MethylcytosineDNA Methylation Patterns among Gut Predomi...CrimsonpublishersMedical
5-MethylcytosineDNA Methylation Patterns among Gut Predominate Commensal Escherichia coli and Lactobacilli from the Balbas and Mazekh Domestic Sheep Breeds by Pepoyan AZ* in Research in Medical &Engineering SciencesWurzbacher, Grimmett, Bärlocher, 2016. Metabarcoding fungal diversity
This document summarizes a study that characterized fungal communities on coarse particulate organic matter (CPOM) like leaves and fine particulate organic matter (FPOM) in a stream in Nova Scotia, Canada using metabarcoding. Maple leaves were exposed in the stream for 4 weeks and 4 FPOM size fractions were collected over the same period by filtration. Pyrosequencing of the samples identified a total of 821 fungal operational taxonomic units, with 726 exclusive to particle samples and 47 to leaf samples. The study aimed to shed light on fungal processing of organic matter in streams and broaden understanding of freshwater fungal diversity.
References 2016
This document provides a list of 26 references used in research related to plant parasitic nematodes and their management. The references are organized alphabetically and include journal articles, book chapters, and theses published between 1951-2016. Many of the references are authored by S.C. Datta and focus on using extracts from Acacia auriculiformis and other plants to control root-knot nematodes infecting mulberry and other crops.
Molecular docking studies of gloriosa superba for anti cancer and anti tuberc...
Gloriosa superba is a medicinal plant generally found in western parts of Tamilnadu and kerala in India. Gloriosa superba having the medicinal property of anticancer, antibacterial, antifungal, anti Tuberculosis and mutagenic activities. In this studies we are going to analysis the anti cancer and anti tuberculosis property of Gloriosa superba by using molecular docking studies. Cancer is a major cause of death and the number of new cases, as well as the number of individuals living with cancer, is expanding continuously. Cervical cancer is one of the most common cancers among women worldwide . Tuberculosis is a common and often deadly infectious disease caused by mycobacteria , usually Mycobacterium tuberculosis in humans. Tuberculosis usually attacks the lungs but can also affect other parts of the body. The phytochemicals of Gloriosa superba are analysed and optimized with the Arguslab to investigate the interactions between the target compounds and the amino acid residues of the E7 and DAHP. All the compound have shown binding pose between from – 3.25 to -7.95 and -7.95 into -11.40 out of ten compound .Chrysophanic acid with E7 protein and Colchicine with DAHP protein show best ligand energy -9.52049 and -7.47679Kcal/mol with 1 and 3 hydrogen bond of distance is 2.3 and 2.2,2.9 and 3.2 respectively .
What's hot (19)
Detection of Genetic variation in tissue culture clones of date palm using IS...
Detection of Genetic variation in tissue culture clones of date palm using IS...
High throughput phenotyping and advanced genotyping reveals QTLs for plant vi...
High throughput phenotyping and advanced genotyping reveals QTLs for plant vi...
Heritable variations of Pteris biaurita L. discovered by ISSR markers in the ...
Heritable variations of Pteris biaurita L. discovered by ISSR markers in the ...
Diversity analysis in chickpea germplasm using SNP and DArT markers
Diversity analysis in chickpea germplasm using SNP and DArT markers
Genetic manipulation of stay-green traits for croop imporvement
Genetic manipulation of stay-green traits for croop imporvement
IUFRO Biodiversity in Forest Ecosystems Conference, Canada 2008
IUFRO Biodiversity in Forest Ecosystems Conference, Canada 2008
Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Car...
Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Car...
Journal of Ethnobotany | Applications of DNA barcoding and future directions ...
Journal of Ethnobotany | Applications of DNA barcoding and future directions ...
Hematological Parameters of three Strains of Local Cocks in Northern Nigeria
Hematological Parameters of three Strains of Local Cocks in Northern Nigeria
Crimson publishers-5-MethylcytosineDNA Methylation Patterns among Gut Predomi...
Crimson publishers-5-MethylcytosineDNA Methylation Patterns among Gut Predomi...
Wurzbacher, Grimmett, Bärlocher, 2016. Metabarcoding fungal diversity
Wurzbacher, Grimmett, Bärlocher, 2016. Metabarcoding fungal diversity
Molecular docking studies of gloriosa superba for anti cancer and anti tuberc...
Molecular docking studies of gloriosa superba for anti cancer and anti tuberc...
Similar to Comparative evaluation of Physical and Chemical mutagens
Genetic Variability, Heritability for Late leaf Spot tolerance and Productivi...
This document summarizes a study on genetic variability, heritability, and traits related to late leaf spot tolerance and productivity in a recombinant inbred line population of groundnut. The study evaluated 106 recombinant inbred lines plus parental lines. High genetic variation was observed for traits related to late leaf spot score, yield, and other components. High heritability and genetic advance were found for traits like late leaf spot score, 100-seed weight, and haulm weight per plant, indicating these are controlled by additive genes and good for selection. Several lines were identified with improved late leaf spot tolerance, early maturity, yield, and other traits.
Induction of Genetic Variability in Soybean in M3 Generation for Quantitative...
Induction of Genetic Variability in Soybean in M3 Generation for Quantitative...SSR Institute of International Journal of Life Sciences
This study investigated the effects of gamma rays and ethyl methane sulphonate (EMS) mutagens on inducing genetic variability in two soybean varieties (PKV-1 and JS-335) for quantitative traits in the M3 generation. The results showed that all mutagenic treatments increased the genotypic and phenotypic coefficient of variation, heritability, and genetic advance for traits like plant height, number of branches, clusters, pods, yield, and 100 grain weight compared to untreated controls. Higher doses of gamma rays (30kR) and EMS concentrations (0.15%) generally induced the most genetic variability. The study concluded that the mutagenic treatments were effective in creating sufficient genetic variability for further selection and02 f ijab 12-724, 011-018
This document presents the results of a study analyzing the kill curves and responses of diploid and tetraploid cotton species to two mutagens, ethyl methanesulfonate (EMS) and gamma rays (γ-rays). Four genotypes from each of three cotton species (Gossypium arboreum, G. barbadense, and G. hirsutum) were treated with varying concentrations of EMS and doses of γ-rays. The results showed significant interactions between species, genotypes, mutagens, and treatment levels for traits like germination, plant height, branching, bolls per plant, boll weight, and lint yield. Optimal mutagenic doses were calculated based on survival rates
biological activity of Calotropis procera against desert locust and migratory...
The treatments based on acetone extracts of Calotropis procera against imagos of Schistocerca gregaria and Locusta migratoria have been
very convincing due to the speed and efficiency of the action of said plant, on mortality and on haemogram of treated locusts.
Mutation Induction for Improvement of Banana (Musa Spp). "Berangan Cv. Intan-...
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
Mutation Induction for Improvement of Banana (Musa Spp.) Berangan Cv. Intan-AAA
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
Sotelo et al. 2008 JEE
This document discusses an experiment that measured the sublethal effects of antibiosis resistance in two Brachiaria hybrids on two important spittlebug pest species in Colombia. The experiment found that:
1) Nymphal survival of both spittlebug species was significantly lower and development time significantly longer when reared on the resistant hybrids compared to the susceptible genotype.
2) Median survival times of resulting adult spittlebugs did not differ based on whether nymphs were reared on resistant or susceptible plants, but reproductive biology was negatively impacted for adults from nymphs reared on the resistant hybrids.
3) The high nymphal mortality and sublethal effects on resulting adults caused by
Study of the cytogenetical effects on meiotic chromosomal abnormalities induc...
Study of the cytogenetical effects on meiotic chromosomal abnormalities induc...SSR Institute of International Journal of Life Sciences
An attempt was made to study the Cytogenetical effects of gamma rays and ethyl methane sulphonate on meiotic chromosomal abnormalities in two cultivars viz., PKV-1 and JS-335. The most frequently observed aberrations in meiosis were univalents, trivalent, multivalents chromosomal fragments, desynapsis of chromosome, laggards, and clumping of chromosomes etc. The physical mutagens were more effective than chemical mutagens. The effect of gamma-rays and ethyl methane sulphonate shows chlorophyll mutations such as Chlorina, Xantha, Albina, and Alboviridis in an M2 generation in both the cultivars. Cultivar JS-335 shows more pronounced effect than cultivar PKV-1. Gamma-rays recorded maximum macro mutations as compared to chemical mutagens (EMS). The frequency and spectrum of morphological mutation indicated that variety JS-335 was more sensitive than PKV-1. Different response of the two varieties to various mutagens was noticed. Key-words- Gamma radiation, EMS, Chromosomal aberrations, Mutagens, Chlorophyll mutationFlow Cytometric Analysis for Ploidy and DNA Content of Banana Variants Induce...
Abstract: Nuclear DNA content of mutated banana plants was determined by using flow cytometric techniques. It is a powerful tool for large scale screening of ploidy levels. Nuclei were isolated from young leaves from (banana mutants & Glycine plants) supplemented with Propidium- iodide (PI) and RNAse. "Glycine max" used as internal reference standard for identifying the nuclear DNA content by FCM. For ploidy estimation DAPI was used. The results showed differences in DNA content between variants indicating the effect of gamma-irradiation on the genotype of these plants. Variants of short plant stature or stunted growth showed great differences in DNA content compared to control (non-irradiated). The phenotypic variations observed at high doses were likely due to changes in the DNA sequences at the chromosomal level. Nuclear DNA contents decreased with an increase of gamma-dose from 20 Gy to 60 Gy. However, there were no significant differences between DNA content at 20 Gy and 30 Gy and also between 40 Gy and 60 Gy, while they were differed significantly from the control. The results showed no significant differences in ploidy level between all samples used (3n); while all selected mutants (variants) showed differences in DNA content.
IMPACT-OF-GENOMICS-ON-AGRICULTUR.pptx
Genomics is impacting agriculture by enabling the study of plant biology on a global scale through genome sequencing and gene expression profiling. Gene expression profiling using microarrays has identified hundreds of genes that are differentially expressed in response to stresses important for agriculture like drought, salt, disease and insects. Proteomics using 2D gel electrophoresis has characterized stress response proteins and been used as a diagnostic tool in plant breeding by identifying protein polymorphisms that are genetic markers or linked to quantitative trait loci (QTLs) controlling traits like drought response.
IMPACT-OF-GENOMICS-ON-AGRICULTUR.pptx
Genomics is impacting agriculture by enabling the study of plant biology on a global scale through genome sequencing and gene expression profiling. Gene expression profiling using microarrays has identified hundreds of genes that are differentially expressed in response to stresses important for agriculture like drought, salt, disease and insects. Proteomics using 2D gel electrophoresis has characterized stress response proteins and identified protein polymorphisms useful for plant breeding as genetic markers. It has also identified protein quantity loci that co-locate with quantitative trait loci, validating candidate genes for traits like drought response in maize.
IMPACT-OF-GENOMICS-ON-AGRICULTUR.pptx
Genomics is impacting agriculture by enabling the study of plant biology on a global scale through genome sequencing and gene expression profiling. Gene expression profiling using microarrays has identified hundreds of genes that are differentially expressed in response to stresses important for agriculture like drought, salt, disease and insects. Proteomics using 2D gel electrophoresis has characterized stress response proteins and identified protein polymorphisms useful for plant breeding as genetic markers. It has also identified protein quantity loci that co-locate with quantitative trait loci, validating candidate genes for traits like drought response in maize.
Micro propagation of stevia rebaudiana bertoni
This document summarizes a study on the micropropagation of Stevia rebaudiana Bertoni, an exotic medicinal plant, through root explants. Researchers found that surface sterilizing root explants with 0.25% mercury chloride solution eliminated contamination and mortality. Shoots were induced on root explants cultured on MS medium supplemented with 1.25mg/L BAP, resulting in 59.5% induction and 3.05cm shoot length. A combination of 1.25mg/L BAP and 0.5mg/L TDZ achieved the highest shoot induction of 89.5% and length of 3.75cm. Subculturing induced multiple shoot proliferation. Roots were induced through culturing micro
Biogenic antimicrobial silver nanoparticles produced by fungi
The document summarizes a study that found two fungi isolated from a Brazilian mangrove forest were able to extracellularly synthesize spherical silver nanoparticles (Ag NP) that were 35±10 nm in size. The Ag NP were characterized through various techniques and showed pronounced antimicrobial activity against pathogenic fungi and bacteria. In particular, Ag NP produced by Aspergillus tubingensis were highly effective at inhibiting Pseudomonas aeruginosa growth. This is the first report of extracellular Ag NP synthesis and antimicrobial properties of Ag NP produced by fungi isolated from a Brazilian mangrove, opening possibilities for obtaining biogenic Ag NP with positive surface potential and new applications.
Heritable variations of Pteris biaurita L. discovered by ISSR markers in the ...
Heritable variations of Pteris biaurita L. discovered by ISSR markers in the ...Journal of Research in Biology
Inter Simple Sequence Repeats (ISSR) markers were utilized to identify the levels of heritable varieties and patterns of the populace structure among the five populaces of Pteris biaurita, a natural fern in India. A comprehensive examination was directed in three replicates at 2013-14 seasons in the Western Ghats, South India. Five wild P. biaurita, accessions (maiden hair) were assessed for genotyping studies. Results demonstrated a pivotal discrepancy among genotypes for they were characterized in view of this uniqueness in four groups by the genetic cluster examination. In this trial, ISSR primers amplified 63 polymorphic groups. In view of the genetic identity data, genotypes were figured and differed from 0.5714 to 0.6984. The percentage of polymorphism indicated predominant genotype that may be utilized for the conservation of species. ISSR appeared to be an obliging marker for prediction of genotype inside a closed group of inter specific populace in the investigation territory.insecticidal effects of eudesmanes from pluchea sagittalis
Eudesmanoids play an important role in the protection of plants against herbivores. Pluchea sagittalis (Lamarck) Cabrera (Asteraceae) is widespread in tropical South America and contains compounds that provide protection against phytophagous insects. In the present work we isolated seven sesquiterpenoids with eudesmane skeletons that were evaluated for their insecticidal activities against Spodoptera frugiperda and Ceratitis capitata, pests that cause serious damage to crops in the Argentine northwest. The Eudesmanes were incorporated at different concentrations to the diet of Spodoptera frugiperda. In the choice test, larval feeding behavior was altered. The eudesmanes 1, 5 and 7 showed the highest activity with feeding election indexes (FEI) of 50, 50, and 72 %, respectively at 200 µg/g of diet. When tested for insecticidal activity using neonate larvae with the no-choice artificial diet bioassays, eudesmane 1 was the most toxic in the larval stage (LD50 177.80 mg/g of diet). Compounds 5 lowered the percentage of adult emergence and produced the most malformations (72%) compared with control. Drastic effects were observed in the oviposition deterrence activity against C. capitata. The maximum oviposition deterrence (87 %) was recorded with eudesmane 5 at dose 30 µg/cm2 of artificial fruit. Finally, eudesmanes 6 and 7 showed significant larval and pupal mortality against the first generation larvae of viable eggs oviposited by females fed with the treated diet (100 µg / g artificial diet).
plant tissue culture ppt
Biodiversity refers to variability among living organisms, including diversity within and between species and ecosystems. Madhya Pradesh has high biodiversity with 30.72% forest cover across 17 forest communities containing 35 tree species. In Indore, most species are vulnerable, threatened, or of least concern while 14% of species are data deficient and 2% are extinct in the wild. Biodiversity loss is increasing due to human activity like habitat loss and climate change. Conservation methods include protected areas, tissue culture of endangered plants, reducing climate change impacts, and education. Tissue culture involves culturing cells or tissues in a nutrient medium and has principles of totipotency and plasticity. It is a method to propagate plants rapidly.
Optimization of sterile male ratio of oriental fruit fly, bactrocera dorsalis...
1) The study aimed to optimize the sterile male ratio of oriental fruit fly (Bactrocera dorsalis) for use in sterile insect technique (SIT) programs. 2) Various pupal ages (5-6 days old) were exposed to gamma radiation doses (30-60 Gray) to determine the sterilizing dose, found to be 60 Gray for 5-day old pupae. 3) Ratios of sterile to wild males (1:1 to 1:9) were tested in field cages with wild females, with the 1:9 ratio found to most suppress wild population characteristics like pupal recovery and emergence based on increased numbers of deformed and non-emerged flies.
mvz_i_v27n2a_2578 (1).pdf
This document describes a study that evaluated the biological, nutritional, and hematoimmune response of juvenile Cherax quadricarinatus (redclaw crayfish) fed different doses of a probiotic mixture. Juveniles were fed doses ranging from 0 to 5×102 μL/100L of a probiotic mixture containing Saccharomyces cerevisiae, Lactobacillus acidophilus and Bacillus species. The study found that biological indicators like weight, growth and survival were best for juveniles fed 4×102 μL of the probiotic. Nutritional parameters like feed conversion and protein efficiency were best for 3×102 μL. The probiotic improved all hematoimmune response indicators measured, with 4×
Reduction in Growth and Biological Pigments Present In Parthenium
Abstract: Parthenium hysterophorus is a well-known weed with numerous harmful impacts. During the present work, aqueous leaf extract of Alstonia scholaris was found allelopathic to the seeds and seedlings of the target weed in laboratory conditions. The allelopathic leaf extract was also found effective against the biological pigments like Chlorophyll a and b followed by protein. Various biochemical tests were performed by which the presence of alcohols, aldehydes and ketones was confirmed in the aqueous allelopathic leaf extract.
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Genetic Variability, Heritability for Late leaf Spot tolerance and Productivi...
Genetic Variability, Heritability for Late leaf Spot tolerance and Productivi...
Induction of Genetic Variability in Soybean in M3 Generation for Quantitative...
Induction of Genetic Variability in Soybean in M3 Generation for Quantitative...
biological activity of Calotropis procera against desert locust and migratory...
biological activity of Calotropis procera against desert locust and migratory...
Mutation Induction for Improvement of Banana (Musa Spp). "Berangan Cv. Intan-...
Mutation Induction for Improvement of Banana (Musa Spp). "Berangan Cv. Intan-...
Mutation Induction for Improvement of Banana (Musa Spp.) Berangan Cv. Intan-AAA
Mutation Induction for Improvement of Banana (Musa Spp.) Berangan Cv. Intan-AAA
Study of the cytogenetical effects on meiotic chromosomal abnormalities induc...
Study of the cytogenetical effects on meiotic chromosomal abnormalities induc...
Flow Cytometric Analysis for Ploidy and DNA Content of Banana Variants Induce...
Flow Cytometric Analysis for Ploidy and DNA Content of Banana Variants Induce...
Biogenic antimicrobial silver nanoparticles produced by fungi
Biogenic antimicrobial silver nanoparticles produced by fungi
Heritable variations of Pteris biaurita L. discovered by ISSR markers in the ...
Heritable variations of Pteris biaurita L. discovered by ISSR markers in the ...
insecticidal effects of eudesmanes from pluchea sagittalis
insecticidal effects of eudesmanes from pluchea sagittalis
Optimization of sterile male ratio of oriental fruit fly, bactrocera dorsalis...
Optimization of sterile male ratio of oriental fruit fly, bactrocera dorsalis...
Reduction in Growth and Biological Pigments Present In Parthenium
Reduction in Growth and Biological Pigments Present In Parthenium
More from Divya S
Interspecific hybridisation among vigna species
Interspecific hybridization involves crossing two different species within the same genus to transfer desirable traits. It has been used in Vigna species like mung bean and urd bean which have complementary traits that could be combined. Successful hybrids have been produced, though barriers like cross incompatibility and hybrid sterility must be overcome. Studies have examined using wild Vigna species as gene sources by crossing them with cultivated species. For example, V. riukiuensis has been used as a bridge between adzuki bean and rice bean. Interspecific hybridization holds promise for broadening the genetic base and improving traits in important pulse crops.
Base editing in plants
Base editing is being used to induce precise mutations in rice in order to accelerate crop improvement. Researchers developed a base editing-mediated gene evolution method to diversify the sequence of the rice acetolactate synthase 1 (OsALS1) gene, which codes for the target of herbicide resistance. Multiple sgRNAs were used to introduce mutations across the OsALS1 locus. Novel mutations were identified that conferred resistance to the herbicide bispyribac-sodium. The resistant mutation was then introduced into an elite rice cultivar through base editing to generate a new herbicide tolerant variety. This demonstrates how base editing can be used to artificially evolve genes and introduce beneficial traits into commercial crops.
Cytogenetics of Saccharum
Cytogenetics of Saccharum officinarum L. and Saccharum spontaneum L. IV. Chromosome Number and Meiosis in S. officinarum X S. spontaneum Hybrids
Principal component analysis
Principal component analysis was conducted on 60 greengram germplasm accessions to determine their relationship and genetic diversity based on various morphological traits including days to flowering, days to maturity, plant height, number of primary branches, number of clusters per plant, number of pods per cluster, number of pods per plant, number of seeds per pod, pod length, 100-seed weight, and seed yield per plant. The study was conducted using a randomized block design at Tamil Nadu Agricultural University in Coimbatore, India.
DNA cloning
DNA cloning techniques allow for the reproduction of DNA fragments. There are two main approaches: cell-based cloning using living cells and in vitro cloning using PCR. The cloning process involves cutting out the gene of interest and a host plasmid with the same restriction enzyme, ligating the gene into the plasmid, transforming bacteria with the new plasmid, and selecting for transformed colonies. Common cloning vectors include plasmids, bacteriophages, cosmids, YACs, and retroviral vectors which allow incorporation of foreign DNA into host genomes. PCR cloning strategies like TA cloning take advantage of Taq polymerase adding extra adenines to PCR products, allowing ligation into vectors with thymidine overhangs.
Multiple trait selection
This document discusses multiple trait selection in plant breeding. It begins by defining multiple trait selection as a selection program that focuses on improving several economically important traits simultaneously. It describes the importance of genetic, environmental, and phenotypic correlations between traits for efficient multiple trait selection. It then discusses three methods for multiple trait selection: tandem selection, independent culling levels, and selection index. For each method it provides details on the approach, advantages, and disadvantages. The selection index method is identified as the most efficient approach for maximizing genetic gain.
PERMAFROST GENE BANK
The document summarizes information about permafrost seed banks, specifically focusing on the Svalbard Global Seed Vault and India's Chang La seed vault. The Svalbard vault was established in 2008 in permafrost in Norway as a backup storage location for seeds from gene banks around the world. It has the capacity to store over 4.5 million different seed samples and is funded by Norway. India's Chang La vault was established in the Himalayas in 2014 as the second permafrost seed bank and contains over 5,000 seed accessions from India as its first deposit to Svalbard. Both vaults take advantage of permafrost conditions for long-term seed storage and preservation of biodiversity.
Biodiversity
This document discusses biodiversity and its conservation. It defines biodiversity as the variety of life forms on Earth, including genes, species, and ecosystems. The importance of biodiversity is explained, as well as threats such as habitat destruction. Methods of conservation are outlined, including both in situ approaches like biosphere reserves, national parks and wildlife sanctuaries, and ex situ approaches such as seed banks and field gene banks. India's protected area network is also briefly discussed. The conclusion emphasizes that conserving biodiversity is important for human survival.
Breeding for resistance to biotic stresses
The document discusses breeding for biotic stress resistance in forage crops. It covers the need for biotic stress resistance in forage crops, mechanisms of disease and insect resistance such as mechanical resistance and antibiosis. Specific examples of disease resistance are given, like crown rust resistance in Italian ryegrass developed through gene pyramiding. The advantages of biotic stress resistance include reducing fungicide use and yield stability, while disadvantages include potential inferior quality and long breeding timeframes.
TILLING- Eco tilling
EcoTILLING is a method for identifying natural mutations and polymorphisms in populations using TILLING techniques. It allows for the detection of point mutations and small insertions/deletions in DNA in a high-throughput but low-cost manner using gel electrophoresis. EcoTILLING has been used to identify allelic variants in genes related to powdery mildew resistance (mlo and Mla) in barley. It has also been applied to identify natural variation in 196 Arabidopsis ecotypes and 41 cottonwood trees. The method shows potential for discovering alleles relevant to salt tolerance in rice varieties and resistance to potato virus Y in pepper varieties.
Biotechnology in forestry
This document discusses the ecological consequences of biodiversity and biotechnology in forestry. It covers topics such as the first genetically modified trees, effects on target and non-target pests from Bt genes, methods of insect and herbicide resistance, flowering modification, lignin modification, and in vitro techniques for conserving forest resources. Biotechnology offers new tools that can complement classical forest improvement methods to better manage forest genetic resources.
Plant lipidomics
Plant lipidomics involves the comprehensive study of all lipids in plants and their roles in cell signaling, membrane structure, and responses to environmental changes. Lipids perform many key functions in plants, including energy storage, cell compartmentalization, and defense. Mass spectrometry-based techniques like MALDI-TOF imaging MS and high-resolution FTMS coupled with HPLC are important analytical methods for identifying and quantifying lipids. These emerging 'lipidomics' approaches combined with genomics and metabolomics can provide insights into lipid pathways and their relationships to plant stress responses and disease. Further development of analytical methods and bioinformatics resources can continue advancing the field of plant lipid research.
Diffused centres of origin
This document discusses the diffuse origins of crops. It notes that early crops like wheat and maize originated in different places than where modern high-yielding varieties were developed. The origins of crop plants occurred over long periods of time in various locations. While Vavilov proposed centers of origin for some crops, evidence suggests crops originated from multiple locations. Archaeobotanical evidence indicates agriculture began independently in different regions through people gradually domesticating wild plants and adding cultigens. Crops and agricultural techniques then diffused from these origins as people migrated and ideas spread. However, agriculture did not always diffuse fully from one culture to another. The document examines archaeological evidence for the spread of pulses from origins in the Fertile Crescent to other
eui gene in rice
1) The eui gene controls elongation of the uppermost internode in rice, allowing panicles to emerge from leaf sheaths. This helps overcome the problem of "panicle enclosure" in hybrid rice production.
2) EUI1 encodes an enzyme that deactivates bioactive GAs in rice. Mutations in EUI1 result in increased bioactive GAs and elongated internodes.
3) HOX12 regulates EUI1 expression - higher HOX12 promotes EUI1 and limits GA levels, while lower HOX12 reduces EUI1 and increases GAs, enhancing panicle exsertion.
PCR based molecular markers
This document discusses various types of PCR-based molecular markers used in genetic analysis. It begins by describing PCR and how it is used to amplify DNA fragments. It then explains different steps of PCR like denaturation, annealing and extension. The document further discusses different types of PCR like reverse transcription PCR, real-time PCR, multiplex PCR etc. It also summarizes various molecular marker techniques derived from PCR including RAPD, AFLP, SSR, SCAR etc and explains the procedure involved in each.
National and international protocol for plant genetic resource management
The document discusses several international agreements related to the conservation and sustainable use of plant genetic resources:
- The Convention on Biological Diversity (CBD) established in 1993 aims to conserve biodiversity and share benefits from genetic resources.
- The Nagoya Protocol aims to ensure fair sharing of benefits from genetic resources.
- The International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA) guarantees food security through conservation and exchange of plant genetic resources. It established a multilateral system for access and benefit sharing involving 64 major crops.
- The Cartagena Protocol addresses transboundary movements of living modified organisms from biotechnology.
The document also discusses India's Biodiversity Act of
Marker Assisted Backcrossing
Marker-assisted backcrossing is used to transfer a desirable trait from a donor parent into a recurrent parent. It involves repeatedly crossing the hybrid progeny with the recurrent parent to recover the recurrent parent's genome while retaining the desired trait. Foreground selection uses markers linked to the target gene to select for its presence, while background and recombinant selection use unlinked markers to recover the recurrent parent's genome and reduce the size of the introgressed donor segment. The process involves multiple generations of backcrossing along with phenotypic, foreground, background, and recombinant selection to efficiently transfer traits into adapted varieties.
Recent Advances in microscopy
Recent Advances in microscopy to view the ultrastructure of the cell
Scanning electron microscopes scan samples with an electron beam to produce images showing surface topography and composition. Cryo-electron tomography vitrifies biological materials and images them in a close-to-native state to acquire 3D information about macromolecular architecture and cellular states. Cellular tomography using cryo-ET can investigate processes like cell adhesion and mitosis as well as observe the cytoskeleton. Phase plate technology provides increased image contrast, allowing observation of thicker cell specimens in situ. Future improvements may allow routine subnanometer resolution cryo-ET observation of cellular features in their native environments.
Distyly- Genetics of Distyly in plants
This document summarizes research on distyly, a polymorphism in plant reproductive structures. It describes distyly involving two floral morphs, one with long stamens and short styles and the other with short stamens and long styles. Genetics research indicates distyly is controlled by a supergene with three tightly linked loci controlling anther position, pollen characteristics, and female structures. The document also reviews models for the evolution of distyly and pathways for its breakdown, such as recombination within the supergene leading to homostyly. It analyzes the phylogenetic distribution of distyly across plant families and the potential effects of polyploidization.
Wide hybridization in cotton
Hybridization between genetically dissimilar cotton species can introduce desirable traits like disease resistance. There are three types of interspecific cotton crosses: fully fertile between species with complete chromosomal homology, partially fertile between species that differ in chromosome number but share some chromosomes, and fully sterile between species without chromosomal homology. Wild cotton species possess traits like hairiness and drought tolerance that can be transferred to cultivated species through distant hybridization. Interspecific cotton crosses can be made fully fertile through colchicine treatment to double chromosomes. This allows transfer of fiber strength from G. anomalum to G. herbaceum.
More from Divya S (20)
National and international protocol for plant genetic resource management
National and international protocol for plant genetic resource management
Recently uploaded
The cost of acquiring information by natural selection
This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Gadgets for management of stored product pests_Dr.UPR.pdf
Insectsplayamajorroleinthedeteriorationoffoodgrainscausingbothquantitativeandqualitativelosses
Wellprovedthatnogranariescanbefilledwithgrainswithoutinsectsastheharvestedproducecontainegg(or)larvae(or)pupae(or)adultinsectinthembecauseoffieldcarryoverinfestationwhichcannotbeavoidedindevelopingcountrieslikeIndia
Simpletechnologiesfortimelydetectionofinsectsinthestoredproduceandtherebyplantimelycontrolmeasures
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
11.1 Role of physical biological in deterioration of grains.pdf
Storagedeteriorationisanyformoflossinquantityandqualityofbio-materials.
Themajorcausesofdeteriorationinstorage
•Physical
•Biological
•Mechanical
•Chemical
Storageonlypreservesquality.Itneverimprovesquality.
Itisadvisabletostartstoragewithqualityfoodproduct.Productwithinitialpoorqualityquicklydepreciates
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
ESR spectroscopy in liquid food and beverages.pptx
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...Advanced-Concepts-Team
Presentation in the Science Coffee of the Advanced Concepts Team of the European Space Agency on the 07.06.2024.
Speaker: Diego Blas (IFAE/ICREA)
Title: Gravitational wave detection with orbital motion of Moon and artificial
Abstract:
In this talk I will describe some recent ideas to find gravitational waves from supermassive black holes or of primordial origin by studying their secular effect on the orbital motion of the Moon or satellites that are laser ranged.Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
Direct Seeded Rice - Climate Smart Agriculture
Direct Seeded Rice - Climate Smart AgricultureInternational Food Policy Research Institute- South Asia Office
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
Recently uploaded (20)
The cost of acquiring information by natural selection
The cost of acquiring information by natural selection
Gadgets for management of stored product pests_Dr.UPR.pdf
Gadgets for management of stored product pests_Dr.UPR.pdf
aziz sancar nobel prize winner: from mardin to nobel
aziz sancar nobel prize winner: from mardin to nobel
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Compexometric titration/Chelatorphy titration/chelating titration
Compexometric titration/Chelatorphy titration/chelating titration
11.1 Role of physical biological in deterioration of grains.pdf
11.1 Role of physical biological in deterioration of grains.pdf
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
ESR spectroscopy in liquid food and beverages.pptx
ESR spectroscopy in liquid food and beverages.pptx
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
Comparative evaluation of Physical and Chemical mutagens
- 1. Comparative Evaluation Of Physical And Chemical Mutagens For Creation Of Variability In The Same Species – Case Studies PRESENTED BY S.DIVYA TAMIL NADU AGRICULTURAL UNIVERSITY, COIMBATORE- 641003
- 2. Casestudy1
- 3. Gladiolus Gladiolus is known worldwide for its attractive spikes and has gained much importance as ‘Queen of bulbous flowers’ The demand of gladiolus is increasing therefore, it needs attention towards genetic improvement. Improvement in any crop depends on the variability available in it Variability can be created through mutation breeding
- 4. Materialsandmethods Corms of gladiolus variety ‘Psittacinus Hybrid’ having diameter of 5-10 cm were treated with different doses of gamma rays (5.5, 6.0, 6.5, 7.0, 7.5, and 8.0 kR) and different concentrations of 0.1, 0.2, 0.3, 0.4 and 0.5% Diethyl Sulphonate (DES) for 3 hours. After the treatment, these corms were immersed in STS solution for 15 minutes Then washed in running tap water for 20 minutes. Then the corms were planted on the raised beds The data were recorded on growth and flowering parameters and statistically analyzed
- 5. Observationsrecorded Vegetative parameters Sprouting percent, Survival percent, Number of tillers, Plant height and Leaf area Flowering parameters Days to flowering, Spike duration, Number of spikes/plant, Spike length, Rachis length, Number of florets/spike and Vase life
- 11. Results Different mutagens like gamma rays and DES, could significantly influence the various vegetative, flowering and yield parameters and also induced some ornamental variants in gladiolus variety ‘Psittacinus hybrid’. Corms treated with DES upto 0.3% produced different favourable variations and abnormalities. Floral abnormalities were also found when irradiated with gamma rays but not any desirable variation was recorded. Colour novelties were produced with different concentrations of DES. Even the quality of spikes improved due to increased spike length and girth of spike at 0.2% and 0.3% DES. Overall, it can be said that DES was more effective as compared to gamma rays for induction of variability.
- 12. Casestudy2 Bhajantri Archana, (2013) Genetic Variability And Improvement Studies In Gladiolus (Gladiolus Hybrids Hort.)
- 13. Materialsandmethods Two gladiolus genotypes (Gladiolus hybrids Hort.) viz., Ethyl Cav Cole and White Prosperity were selected for mutagenic treatments Corms of gladiolus genotypes were treated with chemical mutagen, ethyl methane sulphonate (EMS-CH3CO2OC2H5)- (EMS 0.25%, 0.50% and 0.75%)) and physical mutagen, gamma rays (60Co) -(10 Gy, 20 Gy and 30 Gy).
- 15. Observationsrecorded M1VI generation Sprouting percent, Survival percent, Number of tillers, Plant height and Leaf length Leaf width M1V2 generation Weight of the corm, Diameter of the corm, Days to flowering, Spike duration, Number of spikes/plant, Spike length, Rachis length, Number of florets/spike and Spike girth
- 24. Results Use of lower doses of chemical mutagens (EMS 0.25% ) that do not cause drastic chromosomal damage may be more effective in increasing the amount of variability.
- 25. References Dhawani Patel1, (2018) Sudha Patil, Sanket J. More and Trupti P. Dohiya, Comparative Effect of Physical and Chemical Mutagens in Inducing Variability in Gladiolus Variety ‘Psittacinus Hybrid’ International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01 Bhajantri Archana, (2013) Genetic Variability And Improvement Studies In Gladiolus (Gladiolus Hybrids Hort.)
- 26. THANK YOU