Citrus

2. WHAT IS
MICROPROPAGATION?
Micropropagation is a propagation method that involves
the growth of plant tissues or cells in vitro. It is highly
efficient and can create a large number of plants from a
single specimen. The process involves carefully selected
tissue explants, which are sterilized and placed in
nutrient-rich media.

3. MEDIA FOR
MICROPROPAGATION
For micropropagation, MS-based media are widely
adopted. Generally they are supplemented with sucrose as
a carbon source at a concentration of 30-40 g/l.
media; 2,4-D,
2,4-dichlorophenoxyacetic acid;
BA, benzyl adenine
 NAA, α-naphthalene acetic acid

4. THE BENEFITS OF
MICROPROPAGATION
High Yield Potential
Micropropagation helps grow multiple copies
of the same plant in quick succession, which
boosts yields and maximizes production
efficiency.
Disease-Free Specimens
By starting with disease-free plant material,
growers can propagate new citrus specimens
without the risk of introducing diseases and
pests prevalent in the orchard.

5. Consistent Quality
The use of tissue culture techniques
ensures that every propagated plant
is identical in genetic makeup and
quality to the mother plant.
Shorter Maturation Time
Growers can shorten the maturation
time of new citrus plants by
manipulating plant growth
regulators, temperature, and light
conditions in the micropropagation
environment.

6. MICROPROPAGATION
PROCESS IN DETAIL
Source Plant Selection
Choose a healthy mother plant free of pests and
diseases. The plant should be at its growth peak,
and its tissues should be used as explants.

7.  Epicotyl
 Cotyledons

8. Root segments
Petiole
Leaf segments

9.  Shoot tip
 Meristem
 Explants Sterilization
 Explants are sterilized by immersion in a bleach solution, rinsed with
sterile water, and then treated with alcohol to prevent infections during in
vitro culture.

10. Explant Culture Initiation
The sterilized explants are placed in the in vitro culture
containing nutrient-rich media to initiate plant cell
growth and development.
In Vitro Plantlet Development
After explants have developed, microshoots or plantlets
successfully grown in vitro are removed and transferred to
larger glassware containing nutrient-rich media to
produce a larger number of plants.

11. LABORATORY
REQUIREMENTS FOR
MICROPROPAGATION
Clean Room
Laminar Air Flow
Chemicals & Reagents
Equipment

13.  From one to many propagules rapidly
Multiplication in controlled laboratorium conditions
Continuous propagation year round
 Potential for disease-free propagules

14. Specialized equipment/facilities required
More technical expertise required
Protocols not optimized for all species
Plants produced may not fit industry standards