1. The document discusses sperm DNA fragmentation (SDF), including its origin during spermatogenesis, various etiologies, and methods of assessment.
2. It reviews correlations between SDF and semen parameters as well as clinical outcomes like fertility and pregnancy loss, finding inconsistent relationships.
3. The clinical utility of SDF testing is examined for conditions like infertility, predicting assisted reproduction outcomes, and recurrent miscarriage, though evidence is mixed depending on testing methodology. The document concludes that more evidence is needed before recommending routine SDF testing.
ICSI as it is presently performed is far from an ideal solution because the selection of sperm is based on the judgement of an embryologist, who is looking for the most normal appearing sperm available.
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
ICSI as it is presently performed is far from an ideal solution because the selection of sperm is based on the judgement of an embryologist, who is looking for the most normal appearing sperm available.
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
the objective is to clarify the problem of recurrent implantation failure , regarding the definition, the caused, diagnosis, and management in cases of IVF
Ovarian Reserve Testing in Infertility Dr. Jyoti Agarwal Dr. Sharda JainLifecare Centre
The Best Gametes
Give The Best Result
OVARIAN RESERVE
Plan fertility preservation
Fertility outcome
Response to ovarian stimulation
Predict pregnancy rate
Monitor fertility decline
Fertility after chemotherapy and cancer treatment
the objective is to clarify the problem of recurrent implantation failure , regarding the definition, the caused, diagnosis, and management in cases of IVF
Ovarian Reserve Testing in Infertility Dr. Jyoti Agarwal Dr. Sharda JainLifecare Centre
The Best Gametes
Give The Best Result
OVARIAN RESERVE
Plan fertility preservation
Fertility outcome
Response to ovarian stimulation
Predict pregnancy rate
Monitor fertility decline
Fertility after chemotherapy and cancer treatment
Dr Sujoy Dasgupta was invited to deliver a lecture on "Male Infertility, Antioxidants and Beyond" on 3 February in Yuvacon 2024 organized by the Bengal Obstetric and Gynaecological Society (BOGS). The session was supported by UNS.
Recurrent pregnancy loss (RPL), also referred to as recurrent miscarriage or habitual abortion, is historically defined as 3 consecutive pregnancy losses prior to 20 weeks from the last menstrual period.
This Presentation is made by Dr.Laxmi Shrikhande
Recurrent pregnancy loss is a significant redroductive medical problem, influencing 2%–5% of couples. ... Throughout the years, proof based medications, for example, surgical correction of uterine abnormalities or asprin and heparin for antiphospholipid syndrome have improved the results for couples with repetitive pregnancy loss.
Optimizing male infertility treatment in ART- Dr Parul Katiyar, Max Hospitals...Dr Parul Katiyar
Male factor itself is responsible for infertility in approx 30-40% couples and contributes to infertility in another approx 20%. In many men having normal sperm parameters on semenogrom,
sperms do not function in a manner necessary for fertility and can still cause infertility. This often goes undetected unless specifically sought for. This presentation presents a clinical approach to male factor infertility, specifically related to functional aspects of male fertility.
lecture delivered by Dr Sujoy Dasgupta in a Webinar organized by the Infertility Committee of FOGSI (Federation of Obstetric and Gynaecological Societies of India) and BOGS (Bengal Obstetric and Gynaecological Societiy), held in February, 2021
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Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
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Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Clinical utility of sperm DNA fragmentation tests: 2016
1. Clinical utility of sperm DNA
fragmentation tests
Aboubakr Elnashar
elnashar53@hotmail.com
Benha University Hospital, Egypt
2016
Aboubakr Elnashar
2. CONTENTS
1. Origin of SDF
2. Etiology of SDF
3. Assessment of SDF
4. Correlation with semen parameters
5. Correlation with clinical parameters
6. Clinical utility of SDF tests
7. How to decrease SDF?
Conclusion
7Aboubakr Elnashar
3. 1. ORIGIN OF SDF
(Marin et al, 2012)
First line:
Spermiogenesis*/or spermatogenesis
A breakdown in keys of cell system:
-Apoptosis
-DNA repair
-Chromatin remodelin
Second line:
• Increase radical oxygen species
• Failure of antioxidant defense system during transport
through male reproductive tract
*conversion of round spermatid to elongated spermatid
Aboubakr Elnashar
4. 2. ETIOLOGY OF SDF
(Evgini et al, 2014)
I. Primary Testicular Factors
1. Abnormal germ cell apoptosis
2. Advanced paternal age
3. Exposure to gonadotoxins
4. Sperm protamine deficiency
5. High level of ROS
II. Extratesticular Factors
1. Smoking
2. Radiation
3. Chemotherapy
4. Genital tract inflammation
5. Testicular hyperthermia
6. Varicocele
Aboubakr Elnashar
5. 3. ASSESSMENT OF SDF
TestPrincipleMethod
TUNEL
ISNT
Incorporation of probes
at the site of damage
Direct
SCSA
SCD
Comet
Susceptibility of DBs to
denature in acid
solution
Indirect
Aniline blue
Toluidine blue
Incorporation of probes
to nuclear proteins
Chromatin
incorporation
(Feijo and Esteves, 2014)
Aboubakr Elnashar
7. Normal= 10
Fragmented= 4
SDFI= 4X100/10+4
=28.5%
normal
normal
normal
normal
normal
normal
normal
normal
normal
fragmented
fragmented
fragmented
fragmented
normal
≥30: male infertility
15-30: RM.
≤15: Excellent to Good fertility potential
Aboubakr Elnashar
8. 4. CORRELATION BETWEEN SEMEN
PARAMETERS & SDF
Sperm quality:
(sperm concentration, motility, vitality and morphology)
an inverse correlation
(Lo´pez et al, 2013; Evgini et al, 2014; Komiya et al, 2014)
No significant correlation
(Lin et al, 2008; Xia et al, 2005; Karydis , 2005).
Aboubakr Elnashar
9. Controversy may be attributed to:
1. Variability in the methods used for SDF testing
2. Variability in the methodology and criteria
applied in the analysis of conventional semen
parameters
3. Quality control in semen parameters testing:
rarely mentioned in semen analysis studies
Aboubakr Elnashar
10. Sperm viability
(Samplaski et al, 2015)
(measured by eosin-nigrosin assay)
correlates strongly with SDF.
If viability ≤50%: SDF was ≥ 30%
If viability ≥75%: SDF was ≤30%
If sperm viability≥75%, or ≤ 30%: SDF testing not
necessary.
{SDF testing is more expensive than vitality testing}
Aboubakr Elnashar
11. 5. CORRELATION BETWEEN CLINICAL
PARAMETERS & SDF
(Evgini et al, 2014)
SDF showing correlations with
1. Fertilization rates
2. Embryonic development
3. Implantation
4. Pregnancy
5. Abortion rates
6. Congenital anomalies of the offspring
Aboubakr Elnashar
12. 6. CLINICAL UTILITY OF SDF TESTS
British Fertility Society, 2013
Testing of SDF shows much promise both as
diagnostic test for male infertility and
prognostic test for ART outcomes.
SDF closely associated with fertility outcomes:
Negative relationships with:
fertilization, embryo quality, implantation
Positive relationships with:
miscarriage and childhood diseases.
Aboubakr Elnashar
13. Clinical indications for SDF tests
(Evgini et al, 2014)
1. Prolonged idiopathic male infertility
2. Advanced male age
3. Prolonged exposure to toxic environmental conditions
4. Cancer patients
5. Seminal parameters below the reference range
6. Varicocele
7. Repeated abortions
8. Low fertilization rate or bad quality embryos
9. Implantation failure
Aboubakr Elnashar
15. SDF T should be integrated in routine clinical
practice
(Evgini et al, 2014)
{Strong correlation with:
Several aspects of ART
Further consequences of offspring}
Aboubakr Elnashar
16. 1. Prediction of male fertility
increased SDF: reduced male fertility
(Simon, Lewis; 2011)
Aboubakr Elnashar
17. 2. Prediction of pregnancy outcome in IUI:
Conflicting
DFI ≥30%: lower PR
(Bungum et al, 2007)
Other studies
did not confirm the cutoff
No association
Aboubakr Elnashar
18. 3. Prediction of pregnancy outcome in IVF and
ICSI
low SDF: significantly higher LBR
(Osman et al,2015 MA)
SDF has a greater impact on IVF and ICSI in
reduced ovarian reserve
SDF testing of particular clinical significance in
reduced ovarian reserve
(Jin et al,2015)
Aboubakr Elnashar
19. Not confirmed.
(Coughlan et al, 2015; Zhang et al,2015 MA)
SDF: not associated with pregnancy outcome of
FET in IVF and ICSI.
(Ni et al, 2014)
Why conflicting results?:
{1. Significant differences between test
methodologies
2. Sperm preparation methods in interpreting the
data from SDF tests}
Aboubakr Elnashar
20. 4. Prediction of pregnancy outcome in IVF
SDF: modest but significant reduction in IVF. PR
(Zini et al, 2011, MA, Zhao et al, 2014 MA)
SDFI > 20%: significantly lower LBR in IVF
(Oleszczuk et al, 2016)
Aboubakr Elnashar
21. 5. Prediction of pregnancy outcome in ICSI
No statistically significant difference in LBR between
low and high SDFI
(Osman et al,2015 MA; Oleszczuk et al, 2016)
ICSI might be a preferred method of in vitro
treatment in cases with high SDFI.
Aboubakr Elnashar
22. Diagnostic value of SDF and sperm morphology
for predicting outcome of ICSI.
(Elnashar et al, 2016)
No statistically significant relationship between
SDF before and after semen processing and sperm
morphology and ICSI outcome.
Aboubakr Elnashar
23. 6. Prediction of pregnancy loss
Significant association between SDF and
pregnancy loss after IVF or ICSI
(Zini, 2008, MA, Zhao et al, 2014 MA)
SDF was significantly increased in RM
(17.1% vs. 10.2%)
: possible causes of unexplained RM.
(ZidiJrah et al,2016)
In RM: 30% of men high levels of SDF
(SDFI>15%).
(Leach et al, 2015)
Miscarriage was significantly increased for SDFI >
40%
(Oleszczuk et al, 2016)
Aboubakr Elnashar
24. 7. HOW TO DECREASE SDF
Avoid iatrogenic SDF
(Esteves, 2013)
1. Short abstinence (1day) and serial ejaculation
(Pons et al, 2013)
2. Instruct patients to deliver specimens 1-2h
before ART
3. Process specimens as soon as possible
4. Keep specimens at room Temp using
appropriate culture media
5. Incubation time after processing should not
exceed 4h
6. Thaw cryopreserved specimens just before
performing ART
Aboubakr Elnashar
25. Decrease SDF
1. Oral antioxidant
2. Life style modifications:
stop smoking and wt loss
3. Identify and treat underlying condition:
GTI and varicocele
4. Consider TESA-ICSI
{SDF is significantly lower in testicular specimens
compared with ejaculated one}.
TESA-ICSI is an effective option in oligozoospermia and
high ejaculated SDF.
(Esteves et al, 2015)
Aboubakr Elnashar
26. CONCLUSION
(ASRM, 2013)
There is insufficient evidence to recommend the
Routine use of SDF testing in evaluation
and treatment of infertile couple
{level C}
?????????
For diagnostic test
1. Results must be reproducible
2. Applicable to a given patient
3. Change management of patient
Aboubakr Elnashar
27. 262 lectures
1. My scientific page on face book: 3489 members
2. Slide share: 1228 followers
Aboubakr Elnashar