SlideShare a Scribd company logo
1 of 101
Developed for Science Laboratories
            September 2002
This training presentation has been created for
   those who are required to undergo general
   laboratory safety training and have not taken
   this training with their supervisor. It is also
   recommended for those laboratories that do not
   fall under regulation, but are needing a safety
   laboratory overview.
There are hyperlinks throughout the presentation. Where there is the following
icon additional training is required.             Training
Introduction

Laboratory safety is the key to reducing injury and
illness. There are many exposures in the laboratory
that pose a hazard to your health and you may have
never considered them as a hazard before. It is
important to have proper training so you, as the
employee, are aware of the potential dangers that may
threaten your health or life.
Introduction

As you go through this training module, you will have a
better understanding regarding the concept of safety
and how safety is utilized in every aspect of your life.
The University has an obligation to protect each
student, assistant, faculty and staff. There are also
regulations that pertain to ISU to ensure compliance.
Let’s take a look at the different agencies that are
involved!
Agency Information

Several agencies are involved in safety compliance at
the University. The following agencies impact our
laboratories as far as safety is involved.

    National Institute of Health (NIH)
         In charge of laboratories that use or contain
         recombinant DNA (rDNA). They specify the
         practices for constructing and handling the rDNA.
Agency Information

Centers for Disease Control and Prevention (CDC)
   In charge of laboratories that use or contain infectious
   agents. They are focused on protecting personnel and the
   laboratory environmental from exposure to infectious
   agents. They are also aiming at preventative measures by
   adhering to strict containment.
Agency Information

Occupational Safety and Health Administration (OSHA)

  OSHA primarily monitors Hazardous Materials, Hazard
  Communication, Bloodborne Pathogens, and
  Occupational Exposure to Hazardous Chemicals in
  Laboratories at ISU. OSHA is in charge of monitoring
  work conditions and eliminating physical and health
  hazards at the work place.
Agency Information

Environmental Protection Agency (EPA)

  The EPA is in charge of the wastes that ISU
  generates and stores. Many hazardous wastes are
  used in research laboratories and they pose a threat
  to humans, animals, plants, and the environment.
  There are also biohazardous wastes that are
  regulated and must be properly decontaminated and
  disposed of.
Agency Information

Environmental Health & Safety Office (EHS)

   The EHS office plays a large role in overseeing the
   various activities that take place on campus. They
   interpret laws and regulations, and maintain up-to-date
   records of current health and safety standards. The
   duties range from fire safety and laboratory safety to
   training and emergency response.
Why Training?

Training is by far the most important aspect of
safety. Training not only reduces illness/injury but
also increases worker efficiency and awareness.
Training is required by regulation and ISU has high
regard for fulfilling this commitment. Preventing
hazards and increasing awareness is the goal for
the EHS office.
Who is in Charge of Training?

Everyone on campus is in charge of training. The
individual is responsible for participation of the
training, comprehending the information, and
utilize the information for the specific duties. The
supervisor is in charge of providing proper and
effective training. The EHS office provides
assistance to departments in achieving regulatory
compliance and developing proactive strategies.
Introduction to the Laboratory
Let’s Get Started!

Working in a laboratory can be an
exciting experience. It can also pose
many threats and hazards that a
traditional classroom does not. That
is why it is important to know your
surroundings. Know where the exits
to your room are. There may be more
than one exit which could be critical in
the case of an emergency. Your
supervisor will go over the emergency
action plan including the escape route
procedures for your room.
Know Your Surroundings
It is also recommended to be aware of the fire
extinguishers in location to your laboratory. In order
to fight a fire one must undergo the proper training.
In the event of a fire, the first response is to evacuate
the area and notify the fire department! Any campus
phone will initially direct calls to the ISU Police Dept.
and from there the fire dept. will be dispatched. From
any campus phone just dial 911. Do not wait any
longer than necessary to call, time is of the essence!
Know Your Surroundings

Know where the fire alarm is in proximity to your
 laboratory. Is it right down the hall or is it in the
 stairwell? If there is a fire, a quick response is the
 best response. Have your supervisor show you
 the closest alarm. The fire safety training will
 detail the safety procedures for your lab.



                                            Trainin
                                            g
Know Your Surroundings

Many laboratories contain hazardous substances.
A hazardous substance is defined as a
material/substance that poses a physical or health
hazard. This includes both chemicals and biological
agents.

A Biohazard is defined as any organism that is capable
of replication and is capable of causing disease in
human, animal or plant.

There are differences between a physical hazard and a
 health hazard. Let’s take a look.
Know Your Surroundings

A health hazard has the following
characteristics:
    Carcinogen
    Toxic or highly toxic
    Reproductive Toxins
    Irritants
    Corrosives
    Sensitizers
    Hepatotoxins
    Nephrotoxins
    Neurotoxins
Know Your Surroundings

A physical hazard has the following
characteristics:
    Explosive
    Flammable
    Oxidizer
    Pyrophoric
    Organic peroxide
    Compressed gas
    Combustible liquid
    Unstable (Reactive)
    Water-reactive
Know Your Surroundings
When physical hazards and health hazards
exist, it is very important to know where the
eye wash/safety shower is located.
Unexpected accidents do occur and
knowing where to go at the time of an
emergency can reduce injury/illness.
Know Your Surroundings

First aid kits have a variety of quick relief items.
If your lab has a first aid kit, find out where it is.
If more than first aid is needed, it is
recommended to go to Student Health Services
for further treatment. In an event that would
require more than first aid to be treated, report it
to the EHS office within the next 24 hours.
Know Your Surroundings

When there are chemical, biological, or
radioactive agents being used, an
emergency spill kit should be available. If
there is a spill kit in your lab, find its
location. Further Spill information will be
addressed later in this presentation.
Know Your Surroundings

Each laboratory has a telephone in a
designated area for use. The emergency
contact numbers are posted near the phone
in every laboratory on the ISU campus.
Know What Hazards are Present
Hazards in your Lab


Chemicals can pose a significant hazard. They should be
limited to the use under a properly working fume hood.
Chemicals can release hazardous vapors which not only
harm the environment, but they can be a major health
threat. They must be handled carefully and disposed of
properly.
Hazards in your Lab

When a chemical is in the laboratory, the hazards of
that chemical must be communicated to you.
According to Occupational Safety and Health
Administration (OSHA), a Chemical Hygiene Plan
(CHP) is required to relay information regarding
procedures, equipment, PPE, and work practices
that are capable of protecting employees from
health hazards.

Your supervisor is in charge of providing the
information contained in the CHP to you.
Lab Safety
The following guidelines have been established to
minimize the hazards in a laboratory setting. It is
important to take responsibility for your actions
and to keep in mind that irresponsible acts could
have lasting future effects.

The next portion of the training is simply review
and is meant to remind you of responsible
conduct in a laboratory setting. As the hazards
increase, the risks increase, and the responsibility
must increase.
Lab Attire
      You should remember the following:


 No open-toed shoes
 No shorts unless a lab coat is used
 Restrain hair when working with hazardous
  materials
 Remove protective clothing and gloves in public
 Use the proper Personal Protective Equipment for
  the job
Personal Habits

Personal habits play a large role in
minimizing hazards. The following measures
must be taken:
 Do not eat, drink, smoke, chew gum or apply
  cosmetics, or remove/insert contact lenses
  while in the laboratory
 Do not store food or beverages in the lab or
  in chemical refrigerator
 Do not mouth pipette
 Wash hands before leaving laboratory or
  after handling contaminated material
Safe Practices
   These safe practices should be followed to ensure
                safe working conditions:
 Do not use chipped or cracked glassware
 When working with hazardous materials, have a
  second person nearby
 Know emergency procedures
 Keep the laboratory neat and clean
 Use hazardous chemicals under a fume hood and
  biohazardous materials under a biosafety cabinet
  (BSC)
 Decontaminate as needed
 All procedures should be performed to minimize
  aerosol generation
Know details/location of your vital
   Laboratory Information!
Laboratory Information


Each lab contains an Emergency Action Plan which is
required for emergency situations. This is used to inform
faculty/staff/students of the procedures to follow in the
event of an emergency. This includes the following
information:
Laboratory Information

The Chemical Hygiene Plan (CHP) is similar to the Hazard
Communication Program, but this is specifically for laboratories
with chemicals. This is to relay information regarding procedures,
equipment, PPE, and work practices that are capable of protecting
employees from health hazards. If you are working with acids, it
would be nice to know what personal protective equipment is
necessary and what health hazards are associated with it.
Chemical Hygiene

  According to the laboratory standard, for those labs that have
  chemicals the following training topics must be addressed:

     Methods and observations that may be used to detect
     the presence or release of a hazardous chemicals.
     Physical and health hazards
     Measures employees can take to protect themselves
     from hazards
     Details of the CHP

This training is covered in the Hazard Communication Training
                          Presentation
                                                       Trainin
Chemical Hygiene Plan

Methods and observations used to detect the
presence or release of chemicals will be
specific to your lab. A good indication of the
presence of a chemical is to rely on your
senses. Can you see it or smell it? Is a
monitoring device needed to detect it? This
can be important information if the chemical is
an asphyxiant and pushes oxygen out of the
room you are in.
Chemical Hygiene

Protection against chemicals is a combination of
work procedures or practices, emergency
procedures, and PPE. Some chemicals can only
be handled under certain conditions. It is
important to use proper handling procedures and
practices as advised. The emergency procedures
for chemical accidents is to first evacuate the area
and then notify your supervisor, ISU campus
police and EHS office if necessary.
Chemical Hygiene

Each lab that has chemicals will have a lab
specific Chemical Hygiene Plan. This plan
will detail the procedures and practices for
your specific location. It is your
responsibility to make sure that you review
the CHP.
Labeling

It is important to know as much about a
chemical as possible. The most dangerous
substance is the one that has no label.
Communicating information is essential in the
science field.
MSDS


There are several constituents that are
covered in the Laboratory Standard. Among
these constituents includes labeling and the
MSDS. Let’s look more closely at the content
of the MSDS.
                      MSDS
MSDS

In addition to labeling in a laboratory, the
next most important type of communication
regarding hazards is the MSDS. This is the
acronym for Material Safety Data Sheet.
This will communicate the information
necessary regarding hazards associated
with chemicals and also biological agents.
MSDS

So what is an MSDS? An MSDS is a
document that relays vital information about
certain chemicals and biological agents.


       MSDS Content
         Click here!
Reading the MSDS

 Each component of the MSDS is broken
 down into sections. Each section goes into
 detail about what precautions to take and the
 characteristics of the substance. This is for
 both chemicals and biological agents. See
 the below links for example MSDSs.

http://www.setonresourcecenter.com/MSDSs/comply1.htm
Laboratory Information

Why is an MSDS important? When you know
characteristics about a substance it can aid in
precautionary measure to take when using it.
Also, if there is a spill either on a surface or on
your skin, the MSDS can supply you with the
information needed for first aid.
MSDS

The MSDS to every chemical in your
lab must be available to you. It may be
in a notebook in your labor available
over the internet. Make sure you find
the location of the MSDSs in your room.

               MSDS
Personal Protective
Equipment
What is
                PPE?
PPE is short for personal protective equipment. This is the
equipment that is necessary to protect yourself from
hazardous and biohazardous materials. PPE could be gloves,
safety glasses, lab coat, shoe covers, respirator or any other
item that could protect you from dangerous materials that you
may encounter in the lab.
When to use PPE
Knowing what to use and when to use it is the key to properly
protecting yourself. There could be situations that would be
more of a risk and require more PPE than others. The next few
slides will help you in determining what you should be using!
When to use PPE

Chemical usage poses a variety of hazards.
They can be flammable, corrosive, even
toxic just to name a few. Taking all
precautions to avoid physical and/or health
problems is the number one goal. You can
never be too cautious!
Chemicals & PPE
When chemicals are being used there is
always the possibility of splashing. The proper
PPE to use when chemicals are involved
would include:
Safety glasses
Gloves
Respirator (depending on the chemical and the exposure
                    duration)
        The Permissible Exposure Limit (PEL) for an 8 hr. shift can be
obtained from the PEL/BEL reference guide. Contact EHS if         questions
arise.
Proper Use of Personal
Protective Equipment (PPE)
Use of PPE

Knowing how to properly use PPE can be
the key to adequate protection. Not only
do you want to make sure it is the proper
size for you, but also make sure you are
wearing it properly. If it is too big or too
small, it is not right for you! Let your
supervisor know if you need a different
size.
Use of PPE

If you think you need a respirator talk with
your supervisor or contact EHS (438-8325)
Location & Availability of PPE

Each Lab should have a designated area for the
PPE that is used. The PPE should also be readily
available when working with materials that require it.
 If the proper equipment is not available, you should
not proceed with the work. Notify your supervisor
when you need to order PPE. Give an advanced
notice when possible due to shipping time. Always
consider in advance what you will need when
conducting research.
                                       PPE
What is a Sharp?
What is a “Sharp”?

A sharp is defined as any instrument, tool, or
item that has rigid, acute edges,
protuberances or corners capable of cutting,
piercing, ripping or puncturing such as
syringes, blades, and broken glass. Items
that have the potential for shattering or
breaking are also considered sharps.




          http://www.ehrs.upenn.edu/training/bloodborne/bloodborne.html
Safe Practices


When using a sharp there is a risk of being cut
by the object and possible infection occurring
depending on what the sharp was used for. If
hypodermic needles are used, special
precautions must be taken to reduce the risk of
a needlestick. After use of the needle do not
recap, place directly in the sharp container.
Disposal of Sharps


All sharps must be placed into a rigid,
puncture and leak-resistant container that
is also impervious to moisture. The
sharps container must be labeled either
with “Biohazard” or “Infectious Waste”. Do
not over fill the sharps container.




         http://www.ehrs.upenn.edu/training/bloodborne/bloodborne.html
Disposal of Sharps

When the sharps
container is full it must
be collected by the EHS
office. A waste pick-up
form can be completed
and a collection can be
scheduled. A waste
pick-up form can be
obtained from the EHS
office (438-8325).
BSL-1 Laboratory



                                                     BIOHAZARD
                     All Personal Protective Equipment shall be removed prior to leaving this work area.

                     Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area
                             prohibited in this work area.

                     Name of infectious agent(s):
                     __________________________________________________________________________________

                     __________________________________________________________________________________

                     Transmission route(s):
                     __________________________________________________________________________________

                     Special requirements for entering this area:
                     __________________________________________________________________________________

                     __________________________________________________________________________________
                     Emergency contacts
                     Name: ________________________________ Title: ______________ Phone: _______________
                               ________________________________             _______________             _______________
                               ________________________________             _______________             _______________
                              EH&S office                                    (M-F 8-5) 646-3327 (after hours) 911.
                     __________________________________________________________________________________
                     *Biosafety Level 2 is similar to BSL-1 and is suitable for work involving agents of moderate potential
                     hazard to personnel and the environment. It differs from BSL-1 in that
                     1.Lab personnel have specific training in handling pathogenic agents and directed to competent scientist;
                     2. Access to the lab is limited when work is being conducted:
                     3. Extreme precautions are taken with contaminated sharp items and
                     4. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological
                              safety cabinets or other physical containment equipment

                     New Mexico State University                                                              Date _______
                     Environmental Health & Safety




Signs and Labeling
Labeling
Chemical labeling has been briefly touched on earlier
in the presentation. One must remember that if any
chemical is transferred to a secondary container, this
container must be labeled. If the chemical will be
used by the end of the work shift, then labeling is not
necessary. Good science practices would
encourage you to label all containers.

          So what is included on the Label?
                       Click here
Signs

Signs are a way of communicating important
information. It is a way to heighten
awareness about hazards that exist.

There may be signs on laboratory
refrigerators reminding you that no food or
drink can be stored in it. There may be
radioactive or biological materials that could
possibly be absorbed in food.

There may also be signs which denote that
PPE must be used.
Signs

Labs which use recombinant DNA                BSL-1 Laboratory

and infectious agents must have a
sign posted on the outside of the                                     BIOHAZARD

door. Before someone enters the
                                      All Personal Protective Equipment shall be removed prior to leaving this work area.

                                      Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area
                                              prohibited in this work area.

                                      Name of infectious agent(s):




lab, they will have the information
                                      __________________________________________________________________________________

                                      __________________________________________________________________________________

                                      Transmission route(s):
                                      __________________________________________________________________________________




they need to protect themselves.
                                      Special requirements for entering this area:
                                      __________________________________________________________________________________

                                      __________________________________________________________________________________
                                      Emergency contacts
                                      Name: ________________________________ Title: ______________ Phone: _______________




Always read the signs carefully so
                                                ________________________________             _______________             _______________
                                                ________________________________             _______________             _______________
                                               EH&S office                                    (M-F 8-5) 646-3327 (after hours) 911.
                                      __________________________________________________________________________________
                                      *Biosafety Level 2 is similar to BSL-1 and is suitable for work involving agents of moderate potential
                                      hazard to personnel and the environment. It differs from BSL-1 in that
                                      1.Lab personnel have specific training in handling pathogenic agents and directed to competent scientist;




you know what precautions to
                                      2. Access to the lab is limited when work is being conducted:
                                      3. Extreme precautions are taken with contaminated sharp items and
                                      4. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological
                                               safety cabinets or other physical containment equipment

                                      New Mexico State University                                                              Date _______
                                      Environmental Health & Safety




take.
Practices and Techniques

Responsible practices and techniques are
required when working with hazardous
materials. This is one very important way
to reduce accidents/injuries. Your
supervisor will train you in the responsible
use of hazardous materials specific to
your laboratory.
Safety Equipment

 The Primary Barriers
Safety Equipment

Certain equipment is necessary to achieve
compliance and most importantly to provide
adequate protection.


The safety equipment that is needed is
known as primary and secondary barriers.
Let’s take a look at the difference in the
barriers.
Primary Barriers
Primary barriers are referring to protective
measures including engineering controls. This
includes not only PPE that has already been
covered, but it also includes safety cabinets, fume
hoods, vaccines and autoclaves.



       It is important to know when this equipment
         is to be used and how to properly use it.
Primary Barriers

Let’s look at the following barriers:
    (Besides PPE)


    Fume hood

    Biological Safety Cabinet (BSC)

    Autoclave
Fume Hood


The fume hood is used with chemicals. The
main function is to exhaust the vapors and
gases that are generated in the hood to the
outside. The hood is designed to minimize
your exposure to airborne contaminants. This
is not to be used with biohazardous materials.
Fume Hood Use
When using the fume hood you first need to make
sure the exhaust blower is operating and air is
entering the hood.

Remember, do not put your face inside the hood!

Minimize storage of chemicals in the hood

Clean spills immediately

Work with the sash at the proper operating level
as indicated by the arrows
Biological Safety Cabinet

The biological safety cabinet (BSC) is used as
a containment for infectious agents. The BSC
has a HEPA filter in the exhaust system to
protect the environment and yourself.

The (HEPA) filter is a high efficiency
particulate air filter. It is able to remove
particles at a size of 0.3 µm with an efficiency
of 99.97%. It is also able to remove both
smaller and larger particles.
BSC

There are 3 classes of BSC that are used.
The higher the risk group and biosafety
level, the higher the class of cabinet that is
used.
If there is an infectious agent being used,
whether it is used in research animals or
cultured, it must be manipulated inside the
BSC.
PPE
When using this containment device, remember to also
use the proper personal protective equipment. The
following PPE should be considered depending on the
BSL that is required for the organism that you use.



             Gloves
             Lab Coat
             Shoe Covers
             Safety Glasses
             Respirator
Use of PPE

Always make sure that the BSC has been
decontaminated both before and after use.
Decontamination methods vary depending on the
infectious agent being used. Once the
decontamination of the cabinet is complete, place
the waste in a biohazard bag for autoclave.
Use of BSC

Do not bring contaminated materials out of the
cabinet until they have been surface
decontaminated. If you are unable to
decontaminate, place the material into a
closed container to transfer it to the autoclave
Work Practices for the BSC

Remember to follow the work practices:

  Work in such a way that your face is above the
  front opening

  Wait for 1 minute after placing hands/arms inside
  the cabinet to stabilize the air flow

  Work at least 4 inches from the inside edge of the
  front of the grille
Work Practices for the BSC

Remember to place all materials as far
back in the cabinet as possible


Limit the storage within the cabinet
Autoclave
An autoclave is used to treat infectious
material and recombinant DNA. As a
standard for the University, all material of this
nature must be autoclaved as a safety
precaution. The autoclave is able to render
the material as non-infectious.
Autoclave

The autoclave is able to reach a high
temperature to sterilize the agent. It is
important to know the standard operating
procedures (SOP) for the autoclave. The
SOP is located next to each autoclave. If the
temperature or pressure is inadequate, the
bag is overfilled, or the peak time is not long
enough the material will not be properly
decontaminated.
Autoclave

The autoclave is sensitive to
time, temperature, pressure,
water content, type of container
being used and the type of
waste being sterilized. When
using the autoclave there are a
few important point to
remember in order for the
process to be effective.
Procedures

The material being autoclaved must be placed inside
an autoclave red bag or in a pan (Do not overfill the
bag).

Add ~250-500ml water to the bag or pan for steam to
generate and properly circulate

A spore strip or other approved tape must be placed on
the outside of the bag or pan to verify that the heat
treatment was successful. You will have to verify that a
color change took place.
Procedures

The autoclave must reach a temperature of 121°C
(250 °F) for at least ½ hour at a pressure of 15psi

After successfully being autoclaved, the material can
be placed in regular trash.

Sharps must be incinerated. When the container is
full, a pick up can be scheduled through EHS and
from there Student Health Services will incinerate the
sharps container for proper disposal
Autoclave Log

Each time the autoclave is used, the log must
be completed by the individual using it. The log
is to be located at the site of the autoclave.
The information contained within the log is as
follows:    Date
           Generator Name (P.I.)
           Location
           Time the autoclave reached 121 °C
           Max Temp.
           Pressure
           Type of waste
           If spores or tape were used
           Weight
           Person using autoclave
Autoclave Log


The log is required by EPA and it must be
maintained for 3 years by the department.

The purpose of the log is to ensure that proper
decontamination is taking place

If a new log is needed at the autoclave,
contact the biology department or EHS.
Facility Design

The Secondary Barriers
Secondary Barriers


Secondary barriers are the facility design
and construction. These barriers are to
provide protection for the individuals
outside the lab, the community, and the
environment.
Secondary Barriers


An example of a Secondary barrier would be a
laboratory. It is separate from the classroom and
has limited access.
     The animal room has limited access and is
only available to authorized personnel.
     The autoclave is set away from the normal
traffic.
     The handwashing facilities are located within
     the lab and not accessible by the outside
students.
Decontamination
Decontamination


Decontamination is the removal or
neutralization of toxic agents or the use of
physical or chemical means to remove,
inactivate, or destroy living organisms. This
includes both sterilization and disinfection.
Decontamination

Decontamination is the responsibility of all
laboratory workers. Failure to
decontaminate can result in exposure to
infectious agents which can cause great
illness. Most decontamination can be
done by chemicals. This technique is
used only when autoclaving is not
possible. Continue on to see what would
be best for your lab.
Chemicals

There are a variety of chemicals that can be
used as an effective method of
decontamination. Depending on the agent
being used, the method to use may vary along
with the contact time. For most organisms, a
1:100 chlorine solution for 10-30 minutes is
adequate. The Biosafety Manual has a list of
sterilizers/ disinfectants that can be used.
Autoclave

The autoclave is the most effective method to
use for decontamination purposes. As a
general rule of thumb, autoclave all materials
that are considered infectious agent,
recombinant DNA, or resemble components of
this nature. When in doubt, AUTOCLAVE! If a
material is not capable of autoclave because of
its size, material, or it is stationary, then rely on
chemical disinfectant as a second option.
Spills and Accidents
SOP

Spills and accidents can pose a serious health
and safety threat. When a spill occurs, an
aerosol can be created which can make the
material several times more potent. The best
measure to take in order to protect yourself is to
be prepared. There should be standard
operating procedures for this type of situation in
your lab.
What to Do?

Being able to recognize the hazards, mitigate the spill,
and notifying response authorities can be your best
defense. The first response to a spill should be to
evacuate the immediate area until the scope of the
hazard has been addressed. Seek medical attention if
necessary. Allow sufficient time for the aerosol to settle
before considering entering the room. If you are
responsible for clean up, proper training shall be
addressed.
How to Report

When a spill occurs, it must be reported.
Report to your supervisor all spills. If medical
attention is needed, it is suggested to go to
Student Health Services. All injuries that are
a result of a spill must be reported to EHS.
Waste Management
Waste

Hazardous and biohazardous waste has
special guidelines for proper disposal. It is
important to properly dispose of waste to
ensure human and environmental health.
EPA regulates the waste that is generated
at ISU.
Waste

Waste can be classified as either hazardous or
biohazardous. Let’s take a closer look at the differences.

Hazardous Waste- This is a waste which contains the
characteristics of being any of the following:


             Toxic
             Corrosive
             Ignitable
             Flammable
             Oxidizer
Waste

A biohazardous waste is any waste that is
considered infectious and/or because of its
biological nature it can cause physical or
health hazards in humans, animals, plants or
the environment. This includes recombinant
DNA and other genetically altered organisms
and agents.
Proper Disposal

Waste that is considered biohazardous can be
disposed of in regular trash once it has been
rendered non-infectious. If a biohazard labeled
bag is used, make sure it is either placed in a
secondary bag or a completely new bag that is not
red.




                                http://www.lbl.gov/ehs/Medical/html/biohazardous.htm
Proper Disposal

Hazardous waste must be disposed of
through the Environmental Health & Safety
office. If you need to have hazardous
waste picked up, contact EHS for a pick-up
form.




            EHS 438-8325
You have now completed the General Lab
Safety Training presentation. If you have
any questions please take the time to ask
your supervisor or call EHS.

More Related Content

What's hot

Lecture 8-laboratory accidents and
Lecture 8-laboratory accidents andLecture 8-laboratory accidents and
Lecture 8-laboratory accidents andsaid warsame
 
Chemistry laboratory safety rules
Chemistry laboratory safety rulesChemistry laboratory safety rules
Chemistry laboratory safety rulesSoleta Sua
 
Laboratory Hygiene & Saftey
Laboratory Hygiene & SafteyLaboratory Hygiene & Saftey
Laboratory Hygiene & Safteygeetha T
 
Laboratory hazards, safety and contamination
Laboratory hazards, safety and contaminationLaboratory hazards, safety and contamination
Laboratory hazards, safety and contaminationNadjo Deen
 
Laboratory safety presentation from text book (3rd c h a p t e r) lec 1
Laboratory safety presentation   from text book (3rd c h a p t e r) lec 1Laboratory safety presentation   from text book (3rd c h a p t e r) lec 1
Laboratory safety presentation from text book (3rd c h a p t e r) lec 1biochembiochem
 
Chemicals Safety Presentation
Chemicals Safety PresentationChemicals Safety Presentation
Chemicals Safety PresentationKamarudin Kassim
 
Chemcial handling
 Chemcial handling Chemcial handling
Chemcial handlingReliance
 
Storage and handling of chemicals
Storage and handling of chemicalsStorage and handling of chemicals
Storage and handling of chemicalsSuresh Selvaraj
 
Safety in the laboratory
Safety in the laboratorySafety in the laboratory
Safety in the laboratoryUday Deokate
 
Laboratory chemical waste management practices
Laboratory chemical waste management practices Laboratory chemical waste management practices
Laboratory chemical waste management practices tabirsir
 
Hazardous chemicals handling
Hazardous chemicals handlingHazardous chemicals handling
Hazardous chemicals handlingGamal Abdel Hamid
 
Safe Chemical Handling & Initial Spill Response
Safe Chemical Handling & Initial Spill ResponseSafe Chemical Handling & Initial Spill Response
Safe Chemical Handling & Initial Spill ResponseDavid Horowitz
 

What's hot (20)

Lecture 8-laboratory accidents and
Lecture 8-laboratory accidents andLecture 8-laboratory accidents and
Lecture 8-laboratory accidents and
 
Lab safety
Lab safetyLab safety
Lab safety
 
Chemistry laboratory safety rules
Chemistry laboratory safety rulesChemistry laboratory safety rules
Chemistry laboratory safety rules
 
Laboratory Hygiene & Saftey
Laboratory Hygiene & SafteyLaboratory Hygiene & Saftey
Laboratory Hygiene & Saftey
 
Laboratory hazards, safety and contamination
Laboratory hazards, safety and contaminationLaboratory hazards, safety and contamination
Laboratory hazards, safety and contamination
 
Laboratory safety presentation from text book (3rd c h a p t e r) lec 1
Laboratory safety presentation   from text book (3rd c h a p t e r) lec 1Laboratory safety presentation   from text book (3rd c h a p t e r) lec 1
Laboratory safety presentation from text book (3rd c h a p t e r) lec 1
 
Laboratory safety
Laboratory safetyLaboratory safety
Laboratory safety
 
Lab saftey
Lab saftey Lab saftey
Lab saftey
 
Chemical handling.ppt
Chemical handling.pptChemical handling.ppt
Chemical handling.ppt
 
Chemicals Safety Presentation
Chemicals Safety PresentationChemicals Safety Presentation
Chemicals Safety Presentation
 
Lab safety
Lab safetyLab safety
Lab safety
 
lab safety
 lab safety  lab safety
lab safety
 
Laboratory safety
Laboratory safetyLaboratory safety
Laboratory safety
 
Lab safety good ppt
Lab safety   good pptLab safety   good ppt
Lab safety good ppt
 
Chemcial handling
 Chemcial handling Chemcial handling
Chemcial handling
 
Storage and handling of chemicals
Storage and handling of chemicalsStorage and handling of chemicals
Storage and handling of chemicals
 
Safety in the laboratory
Safety in the laboratorySafety in the laboratory
Safety in the laboratory
 
Laboratory chemical waste management practices
Laboratory chemical waste management practices Laboratory chemical waste management practices
Laboratory chemical waste management practices
 
Hazardous chemicals handling
Hazardous chemicals handlingHazardous chemicals handling
Hazardous chemicals handling
 
Safe Chemical Handling & Initial Spill Response
Safe Chemical Handling & Initial Spill ResponseSafe Chemical Handling & Initial Spill Response
Safe Chemical Handling & Initial Spill Response
 

Viewers also liked

Laboratory safety manual fantastic
Laboratory safety manual fantasticLaboratory safety manual fantastic
Laboratory safety manual fantasticChala Dandessa
 
Unit 1, Lesson 1.6 - Safety in the Laboratory
Unit 1, Lesson 1.6 - Safety in the LaboratoryUnit 1, Lesson 1.6 - Safety in the Laboratory
Unit 1, Lesson 1.6 - Safety in the Laboratoryjudan1970
 
Laboratory Safety, Biomedical Waste & Its Management
Laboratory  Safety, Biomedical Waste & Its ManagementLaboratory  Safety, Biomedical Waste & Its Management
Laboratory Safety, Biomedical Waste & Its ManagementArun Babu
 
Lab safety
Lab safetyLab safety
Lab safetyitsebo
 
OSHA Hazard Communication and Global Harmonization System (GHS)
OSHA Hazard Communication and Global Harmonization System (GHS)OSHA Hazard Communication and Global Harmonization System (GHS)
OSHA Hazard Communication and Global Harmonization System (GHS)Elena Fracassa
 
Onlabs: 3D Game for Distance Laboratory
Onlabs: 3D Game for Distance Laboratory Onlabs: 3D Game for Distance Laboratory
Onlabs: 3D Game for Distance Laboratory EADTU
 
1. laboratory safety protocol
1. laboratory safety  protocol1. laboratory safety  protocol
1. laboratory safety protocolZari Esa M. Saleh
 
MU Laboratory Safety
MU Laboratory SafetyMU Laboratory Safety
MU Laboratory Safetytblackley
 
Fire Safety & Extinguisher Safety Training
Fire Safety & Extinguisher Safety TrainingFire Safety & Extinguisher Safety Training
Fire Safety & Extinguisher Safety TrainingPhillipLaraway
 
Fire Extinguisher safety training
Fire Extinguisher safety trainingFire Extinguisher safety training
Fire Extinguisher safety trainingShowa Denko Carbon
 
Wayne State University Laboratory Safety Training
Wayne State University Laboratory Safety TrainingWayne State University Laboratory Safety Training
Wayne State University Laboratory Safety TrainingElena Fracassa
 

Viewers also liked (20)

Laboratory safety manual fantastic
Laboratory safety manual fantasticLaboratory safety manual fantastic
Laboratory safety manual fantastic
 
Chemical Safety for K-12 Science Teachers
Chemical Safety for K-12 Science TeachersChemical Safety for K-12 Science Teachers
Chemical Safety for K-12 Science Teachers
 
Unit 1, Lesson 1.6 - Safety in the Laboratory
Unit 1, Lesson 1.6 - Safety in the LaboratoryUnit 1, Lesson 1.6 - Safety in the Laboratory
Unit 1, Lesson 1.6 - Safety in the Laboratory
 
Laboratory safety
Laboratory safetyLaboratory safety
Laboratory safety
 
Laboratory Safety, Biomedical Waste & Its Management
Laboratory  Safety, Biomedical Waste & Its ManagementLaboratory  Safety, Biomedical Waste & Its Management
Laboratory Safety, Biomedical Waste & Its Management
 
Lab safety
Lab safetyLab safety
Lab safety
 
Laboratory Safety Training by UVU
Laboratory Safety Training by UVULaboratory Safety Training by UVU
Laboratory Safety Training by UVU
 
OSHA Hazard Communication and Global Harmonization System (GHS)
OSHA Hazard Communication and Global Harmonization System (GHS)OSHA Hazard Communication and Global Harmonization System (GHS)
OSHA Hazard Communication and Global Harmonization System (GHS)
 
Onlabs: 3D Game for Distance Laboratory
Onlabs: 3D Game for Distance Laboratory Onlabs: 3D Game for Distance Laboratory
Onlabs: 3D Game for Distance Laboratory
 
Fire safety
Fire safetyFire safety
Fire safety
 
PPT KEAMANAN LABORATORIUM
PPT KEAMANAN LABORATORIUMPPT KEAMANAN LABORATORIUM
PPT KEAMANAN LABORATORIUM
 
Hazard awareness
Hazard awarenessHazard awareness
Hazard awareness
 
Fire safety
Fire safetyFire safety
Fire safety
 
1. laboratory safety protocol
1. laboratory safety  protocol1. laboratory safety  protocol
1. laboratory safety protocol
 
MU Laboratory Safety
MU Laboratory SafetyMU Laboratory Safety
MU Laboratory Safety
 
Fire safety training
Fire safety trainingFire safety training
Fire safety training
 
Fire & safety_signs
Fire & safety_signsFire & safety_signs
Fire & safety_signs
 
Fire Safety & Extinguisher Safety Training
Fire Safety & Extinguisher Safety TrainingFire Safety & Extinguisher Safety Training
Fire Safety & Extinguisher Safety Training
 
Fire Extinguisher safety training
Fire Extinguisher safety trainingFire Extinguisher safety training
Fire Extinguisher safety training
 
Wayne State University Laboratory Safety Training
Wayne State University Laboratory Safety TrainingWayne State University Laboratory Safety Training
Wayne State University Laboratory Safety Training
 

Similar to General Laboratory Safety Training

General laboratory safety training 1
General laboratory safety training 1General laboratory safety training 1
General laboratory safety training 1Ibrahim Khleifat
 
gen-lab-training.ppt
gen-lab-training.pptgen-lab-training.ppt
gen-lab-training.pptssuser627f7c
 
ChemicalLaboratorySafetyTraining.pdf
ChemicalLaboratorySafetyTraining.pdfChemicalLaboratorySafetyTraining.pdf
ChemicalLaboratorySafetyTraining.pdfJagdeep41
 
General Laboratory Safety full slideshow.pptx
General Laboratory Safety  full slideshow.pptxGeneral Laboratory Safety  full slideshow.pptx
General Laboratory Safety full slideshow.pptxAhnaf maznun
 
Safety and Infection control in hematology laboratory
Safety and Infection control in hematology laboratorySafety and Infection control in hematology laboratory
Safety and Infection control in hematology laboratoryNegash Alamin
 
Lect.1 | د.سماح م١ ⚠️.pdf
Lect.1 | د.سماح م١ ⚠️.pdfLect.1 | د.سماح م١ ⚠️.pdf
Lect.1 | د.سماح م١ ⚠️.pdfyaAllah5
 
Lab safety Universal precautions,Patient safety, staff safety .pptx
Lab safety Universal precautions,Patient safety, staff safety .pptxLab safety Universal precautions,Patient safety, staff safety .pptx
Lab safety Universal precautions,Patient safety, staff safety .pptxAishwaryaRamalingam1
 
BIOSAFETY_PRESENTATION_KATO 2022.ppt
BIOSAFETY_PRESENTATION_KATO 2022.pptBIOSAFETY_PRESENTATION_KATO 2022.ppt
BIOSAFETY_PRESENTATION_KATO 2022.pptGerald600591
 
Biosafety and ethics in Molecular biology laboratory
Biosafety and ethics in Molecular biology laboratoryBiosafety and ethics in Molecular biology laboratory
Biosafety and ethics in Molecular biology laboratoryAyesha Aftab
 
BPP Practice Occupational Health and Safety Procedures
BPP Practice Occupational Health and Safety ProceduresBPP Practice Occupational Health and Safety Procedures
BPP Practice Occupational Health and Safety ProceduresDanielle Mendoza
 
Industrial Safety.pdf SAFTY AND INDUSTRIAL
Industrial Safety.pdf SAFTY AND INDUSTRIALIndustrial Safety.pdf SAFTY AND INDUSTRIAL
Industrial Safety.pdf SAFTY AND INDUSTRIALsarahrashid24
 
Intro and personal safety
Intro and personal safetyIntro and personal safety
Intro and personal safetySaid Al Jaafari
 
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...Guntamukkala Brahmayesu
 
WORPLACE HAZARDS & PRECAUTIONS.pptx
WORPLACE HAZARDS & PRECAUTIONS.pptxWORPLACE HAZARDS & PRECAUTIONS.pptx
WORPLACE HAZARDS & PRECAUTIONS.pptxSyed Neyaz Ahmad
 
Biosafety and biosecurity biosafety levels
Biosafety and biosecurity  biosafety levelsBiosafety and biosecurity  biosafety levels
Biosafety and biosecurity biosafety levelsSilviaMachado52
 
Chemical Injury.pptx
Chemical Injury.pptxChemical Injury.pptx
Chemical Injury.pptxNajads1
 
Laboratory safety 2017
Laboratory safety 2017 Laboratory safety 2017
Laboratory safety 2017 John Newquist
 

Similar to General Laboratory Safety Training (20)

General laboratory safety training 1
General laboratory safety training 1General laboratory safety training 1
General laboratory safety training 1
 
gen-lab-training.ppt
gen-lab-training.pptgen-lab-training.ppt
gen-lab-training.ppt
 
ChemicalLaboratorySafetyTraining.pdf
ChemicalLaboratorySafetyTraining.pdfChemicalLaboratorySafetyTraining.pdf
ChemicalLaboratorySafetyTraining.pdf
 
General Laboratory Safety full slideshow.pptx
General Laboratory Safety  full slideshow.pptxGeneral Laboratory Safety  full slideshow.pptx
General Laboratory Safety full slideshow.pptx
 
Chemical Safety.pptx
 Chemical Safety.pptx Chemical Safety.pptx
Chemical Safety.pptx
 
Safety and Infection control in hematology laboratory
Safety and Infection control in hematology laboratorySafety and Infection control in hematology laboratory
Safety and Infection control in hematology laboratory
 
Lect.1 | د.سماح م١ ⚠️.pdf
Lect.1 | د.سماح م١ ⚠️.pdfLect.1 | د.سماح م١ ⚠️.pdf
Lect.1 | د.سماح م١ ⚠️.pdf
 
Lab safety Universal precautions,Patient safety, staff safety .pptx
Lab safety Universal precautions,Patient safety, staff safety .pptxLab safety Universal precautions,Patient safety, staff safety .pptx
Lab safety Universal precautions,Patient safety, staff safety .pptx
 
BIOSAFETY_PRESENTATION_KATO 2022.ppt
BIOSAFETY_PRESENTATION_KATO 2022.pptBIOSAFETY_PRESENTATION_KATO 2022.ppt
BIOSAFETY_PRESENTATION_KATO 2022.ppt
 
Biosafety and ethics in Molecular biology laboratory
Biosafety and ethics in Molecular biology laboratoryBiosafety and ethics in Molecular biology laboratory
Biosafety and ethics in Molecular biology laboratory
 
BPP Practice Occupational Health and Safety Procedures
BPP Practice Occupational Health and Safety ProceduresBPP Practice Occupational Health and Safety Procedures
BPP Practice Occupational Health and Safety Procedures
 
Industrial Safety.pdf SAFTY AND INDUSTRIAL
Industrial Safety.pdf SAFTY AND INDUSTRIALIndustrial Safety.pdf SAFTY AND INDUSTRIAL
Industrial Safety.pdf SAFTY AND INDUSTRIAL
 
Cookery 3
Cookery 3Cookery 3
Cookery 3
 
Intro and personal safety
Intro and personal safetyIntro and personal safety
Intro and personal safety
 
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
Lab safety and regulations by dr.brahmesh, PG BIOCHEMISTRY, AMC, VIZAG, AP, I...
 
OH & Safety
OH & SafetyOH & Safety
OH & Safety
 
WORPLACE HAZARDS & PRECAUTIONS.pptx
WORPLACE HAZARDS & PRECAUTIONS.pptxWORPLACE HAZARDS & PRECAUTIONS.pptx
WORPLACE HAZARDS & PRECAUTIONS.pptx
 
Biosafety and biosecurity biosafety levels
Biosafety and biosecurity  biosafety levelsBiosafety and biosecurity  biosafety levels
Biosafety and biosecurity biosafety levels
 
Chemical Injury.pptx
Chemical Injury.pptxChemical Injury.pptx
Chemical Injury.pptx
 
Laboratory safety 2017
Laboratory safety 2017 Laboratory safety 2017
Laboratory safety 2017
 

More from John Gavazzi

Introduction to Moral Injury, Theory & Practice
Introduction to Moral Injury, Theory & PracticeIntroduction to Moral Injury, Theory & Practice
Introduction to Moral Injury, Theory & PracticeJohn Gavazzi
 
Ethical reasoning: decision science, biases, and errors
Ethical reasoning: decision science, biases, and errorsEthical reasoning: decision science, biases, and errors
Ethical reasoning: decision science, biases, and errorsJohn Gavazzi
 
Learning Telehealth in the Midst of a Pandemic
Learning Telehealth in the Midst of a PandemicLearning Telehealth in the Midst of a Pandemic
Learning Telehealth in the Midst of a PandemicJohn Gavazzi
 
The Assessment, Management, and Treatment of Suicidal Patients
The Assessment, Management, and Treatment of Suicidal PatientsThe Assessment, Management, and Treatment of Suicidal Patients
The Assessment, Management, and Treatment of Suicidal PatientsJohn Gavazzi
 
Intentional Ethics: Decision-making, Telehealth, and Social Media
Intentional Ethics: Decision-making, Telehealth, and Social MediaIntentional Ethics: Decision-making, Telehealth, and Social Media
Intentional Ethics: Decision-making, Telehealth, and Social MediaJohn Gavazzi
 
Social Media, Ethics and Professional Education
Social Media, Ethics and Professional EducationSocial Media, Ethics and Professional Education
Social Media, Ethics and Professional EducationJohn Gavazzi
 
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...John Gavazzi
 
The darker side of ethics and morality in psychotherapy.pptx
The darker side of ethics and morality in psychotherapy.pptxThe darker side of ethics and morality in psychotherapy.pptx
The darker side of ethics and morality in psychotherapy.pptxJohn Gavazzi
 
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...John Gavazzi
 
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...John Gavazzi
 
Leadership, advocacy, and ethics
Leadership, advocacy, and ethicsLeadership, advocacy, and ethics
Leadership, advocacy, and ethicsJohn Gavazzi
 
Child Abuse Reporting Guidelines: Ethical and Legal Issues
Child Abuse Reporting Guidelines: Ethical and Legal IssuesChild Abuse Reporting Guidelines: Ethical and Legal Issues
Child Abuse Reporting Guidelines: Ethical and Legal IssuesJohn Gavazzi
 
Act 31 Training for Licensed Professionals in Pennsylvania
Act 31 Training for Licensed Professionals in PennsylvaniaAct 31 Training for Licensed Professionals in Pennsylvania
Act 31 Training for Licensed Professionals in PennsylvaniaJohn Gavazzi
 
Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...
Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...
Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...John Gavazzi
 
Legal, Clinical, Risk Management and Ethical Issues in Mental Health
Legal, Clinical, Risk Management and Ethical Issues in Mental HealthLegal, Clinical, Risk Management and Ethical Issues in Mental Health
Legal, Clinical, Risk Management and Ethical Issues in Mental HealthJohn Gavazzi
 
Ethical and Moral Foundations in Mental Health Treatment
Ethical and Moral Foundations in Mental Health TreatmentEthical and Moral Foundations in Mental Health Treatment
Ethical and Moral Foundations in Mental Health TreatmentJohn Gavazzi
 
Ethics in Practice: Mandated Reporting, Boundaries, and Decision-making
Ethics in Practice: Mandated Reporting, Boundaries, and Decision-makingEthics in Practice: Mandated Reporting, Boundaries, and Decision-making
Ethics in Practice: Mandated Reporting, Boundaries, and Decision-makingJohn Gavazzi
 
Dark side of ethics podcast: False Risk management strategies
Dark side of ethics podcast: False Risk management strategiesDark side of ethics podcast: False Risk management strategies
Dark side of ethics podcast: False Risk management strategiesJohn Gavazzi
 

More from John Gavazzi (20)

Introduction to Moral Injury, Theory & Practice
Introduction to Moral Injury, Theory & PracticeIntroduction to Moral Injury, Theory & Practice
Introduction to Moral Injury, Theory & Practice
 
Ethical reasoning: decision science, biases, and errors
Ethical reasoning: decision science, biases, and errorsEthical reasoning: decision science, biases, and errors
Ethical reasoning: decision science, biases, and errors
 
Learning Telehealth in the Midst of a Pandemic
Learning Telehealth in the Midst of a PandemicLearning Telehealth in the Midst of a Pandemic
Learning Telehealth in the Midst of a Pandemic
 
The Assessment, Management, and Treatment of Suicidal Patients
The Assessment, Management, and Treatment of Suicidal PatientsThe Assessment, Management, and Treatment of Suicidal Patients
The Assessment, Management, and Treatment of Suicidal Patients
 
Intentional Ethics: Decision-making, Telehealth, and Social Media
Intentional Ethics: Decision-making, Telehealth, and Social MediaIntentional Ethics: Decision-making, Telehealth, and Social Media
Intentional Ethics: Decision-making, Telehealth, and Social Media
 
Social Media, Ethics and Professional Education
Social Media, Ethics and Professional EducationSocial Media, Ethics and Professional Education
Social Media, Ethics and Professional Education
 
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
 
The darker side of ethics and morality in psychotherapy.pptx
The darker side of ethics and morality in psychotherapy.pptxThe darker side of ethics and morality in psychotherapy.pptx
The darker side of ethics and morality in psychotherapy.pptx
 
Just ethics
Just ethicsJust ethics
Just ethics
 
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
 
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
Ethics and Skills for Psychologist as Supervisor: Post-Doctoral Supervision i...
 
Leadership, advocacy, and ethics
Leadership, advocacy, and ethicsLeadership, advocacy, and ethics
Leadership, advocacy, and ethics
 
Ethics hell
Ethics hellEthics hell
Ethics hell
 
Child Abuse Reporting Guidelines: Ethical and Legal Issues
Child Abuse Reporting Guidelines: Ethical and Legal IssuesChild Abuse Reporting Guidelines: Ethical and Legal Issues
Child Abuse Reporting Guidelines: Ethical and Legal Issues
 
Act 31 Training for Licensed Professionals in Pennsylvania
Act 31 Training for Licensed Professionals in PennsylvaniaAct 31 Training for Licensed Professionals in Pennsylvania
Act 31 Training for Licensed Professionals in Pennsylvania
 
Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...
Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...
Closing a Professional Practice: Clinical, Ethical and Practical Consideratio...
 
Legal, Clinical, Risk Management and Ethical Issues in Mental Health
Legal, Clinical, Risk Management and Ethical Issues in Mental HealthLegal, Clinical, Risk Management and Ethical Issues in Mental Health
Legal, Clinical, Risk Management and Ethical Issues in Mental Health
 
Ethical and Moral Foundations in Mental Health Treatment
Ethical and Moral Foundations in Mental Health TreatmentEthical and Moral Foundations in Mental Health Treatment
Ethical and Moral Foundations in Mental Health Treatment
 
Ethics in Practice: Mandated Reporting, Boundaries, and Decision-making
Ethics in Practice: Mandated Reporting, Boundaries, and Decision-makingEthics in Practice: Mandated Reporting, Boundaries, and Decision-making
Ethics in Practice: Mandated Reporting, Boundaries, and Decision-making
 
Dark side of ethics podcast: False Risk management strategies
Dark side of ethics podcast: False Risk management strategiesDark side of ethics podcast: False Risk management strategies
Dark side of ethics podcast: False Risk management strategies
 

General Laboratory Safety Training

  • 1. Developed for Science Laboratories September 2002
  • 2. This training presentation has been created for those who are required to undergo general laboratory safety training and have not taken this training with their supervisor. It is also recommended for those laboratories that do not fall under regulation, but are needing a safety laboratory overview. There are hyperlinks throughout the presentation. Where there is the following icon additional training is required. Training
  • 3. Introduction Laboratory safety is the key to reducing injury and illness. There are many exposures in the laboratory that pose a hazard to your health and you may have never considered them as a hazard before. It is important to have proper training so you, as the employee, are aware of the potential dangers that may threaten your health or life.
  • 4. Introduction As you go through this training module, you will have a better understanding regarding the concept of safety and how safety is utilized in every aspect of your life. The University has an obligation to protect each student, assistant, faculty and staff. There are also regulations that pertain to ISU to ensure compliance. Let’s take a look at the different agencies that are involved!
  • 5. Agency Information Several agencies are involved in safety compliance at the University. The following agencies impact our laboratories as far as safety is involved. National Institute of Health (NIH) In charge of laboratories that use or contain recombinant DNA (rDNA). They specify the practices for constructing and handling the rDNA.
  • 6. Agency Information Centers for Disease Control and Prevention (CDC) In charge of laboratories that use or contain infectious agents. They are focused on protecting personnel and the laboratory environmental from exposure to infectious agents. They are also aiming at preventative measures by adhering to strict containment.
  • 7. Agency Information Occupational Safety and Health Administration (OSHA) OSHA primarily monitors Hazardous Materials, Hazard Communication, Bloodborne Pathogens, and Occupational Exposure to Hazardous Chemicals in Laboratories at ISU. OSHA is in charge of monitoring work conditions and eliminating physical and health hazards at the work place.
  • 8. Agency Information Environmental Protection Agency (EPA) The EPA is in charge of the wastes that ISU generates and stores. Many hazardous wastes are used in research laboratories and they pose a threat to humans, animals, plants, and the environment. There are also biohazardous wastes that are regulated and must be properly decontaminated and disposed of.
  • 9. Agency Information Environmental Health & Safety Office (EHS) The EHS office plays a large role in overseeing the various activities that take place on campus. They interpret laws and regulations, and maintain up-to-date records of current health and safety standards. The duties range from fire safety and laboratory safety to training and emergency response.
  • 10. Why Training? Training is by far the most important aspect of safety. Training not only reduces illness/injury but also increases worker efficiency and awareness. Training is required by regulation and ISU has high regard for fulfilling this commitment. Preventing hazards and increasing awareness is the goal for the EHS office.
  • 11. Who is in Charge of Training? Everyone on campus is in charge of training. The individual is responsible for participation of the training, comprehending the information, and utilize the information for the specific duties. The supervisor is in charge of providing proper and effective training. The EHS office provides assistance to departments in achieving regulatory compliance and developing proactive strategies.
  • 12. Introduction to the Laboratory
  • 13. Let’s Get Started! Working in a laboratory can be an exciting experience. It can also pose many threats and hazards that a traditional classroom does not. That is why it is important to know your surroundings. Know where the exits to your room are. There may be more than one exit which could be critical in the case of an emergency. Your supervisor will go over the emergency action plan including the escape route procedures for your room.
  • 14. Know Your Surroundings It is also recommended to be aware of the fire extinguishers in location to your laboratory. In order to fight a fire one must undergo the proper training. In the event of a fire, the first response is to evacuate the area and notify the fire department! Any campus phone will initially direct calls to the ISU Police Dept. and from there the fire dept. will be dispatched. From any campus phone just dial 911. Do not wait any longer than necessary to call, time is of the essence!
  • 15. Know Your Surroundings Know where the fire alarm is in proximity to your laboratory. Is it right down the hall or is it in the stairwell? If there is a fire, a quick response is the best response. Have your supervisor show you the closest alarm. The fire safety training will detail the safety procedures for your lab. Trainin g
  • 16. Know Your Surroundings Many laboratories contain hazardous substances. A hazardous substance is defined as a material/substance that poses a physical or health hazard. This includes both chemicals and biological agents. A Biohazard is defined as any organism that is capable of replication and is capable of causing disease in human, animal or plant. There are differences between a physical hazard and a health hazard. Let’s take a look.
  • 17. Know Your Surroundings A health hazard has the following characteristics: Carcinogen Toxic or highly toxic Reproductive Toxins Irritants Corrosives Sensitizers Hepatotoxins Nephrotoxins Neurotoxins
  • 18. Know Your Surroundings A physical hazard has the following characteristics: Explosive Flammable Oxidizer Pyrophoric Organic peroxide Compressed gas Combustible liquid Unstable (Reactive) Water-reactive
  • 19. Know Your Surroundings When physical hazards and health hazards exist, it is very important to know where the eye wash/safety shower is located. Unexpected accidents do occur and knowing where to go at the time of an emergency can reduce injury/illness.
  • 20. Know Your Surroundings First aid kits have a variety of quick relief items. If your lab has a first aid kit, find out where it is. If more than first aid is needed, it is recommended to go to Student Health Services for further treatment. In an event that would require more than first aid to be treated, report it to the EHS office within the next 24 hours.
  • 21. Know Your Surroundings When there are chemical, biological, or radioactive agents being used, an emergency spill kit should be available. If there is a spill kit in your lab, find its location. Further Spill information will be addressed later in this presentation.
  • 22. Know Your Surroundings Each laboratory has a telephone in a designated area for use. The emergency contact numbers are posted near the phone in every laboratory on the ISU campus.
  • 23. Know What Hazards are Present
  • 24. Hazards in your Lab Chemicals can pose a significant hazard. They should be limited to the use under a properly working fume hood. Chemicals can release hazardous vapors which not only harm the environment, but they can be a major health threat. They must be handled carefully and disposed of properly.
  • 25. Hazards in your Lab When a chemical is in the laboratory, the hazards of that chemical must be communicated to you. According to Occupational Safety and Health Administration (OSHA), a Chemical Hygiene Plan (CHP) is required to relay information regarding procedures, equipment, PPE, and work practices that are capable of protecting employees from health hazards. Your supervisor is in charge of providing the information contained in the CHP to you.
  • 26. Lab Safety The following guidelines have been established to minimize the hazards in a laboratory setting. It is important to take responsibility for your actions and to keep in mind that irresponsible acts could have lasting future effects. The next portion of the training is simply review and is meant to remind you of responsible conduct in a laboratory setting. As the hazards increase, the risks increase, and the responsibility must increase.
  • 27. Lab Attire You should remember the following:  No open-toed shoes  No shorts unless a lab coat is used  Restrain hair when working with hazardous materials  Remove protective clothing and gloves in public  Use the proper Personal Protective Equipment for the job
  • 28. Personal Habits Personal habits play a large role in minimizing hazards. The following measures must be taken:  Do not eat, drink, smoke, chew gum or apply cosmetics, or remove/insert contact lenses while in the laboratory  Do not store food or beverages in the lab or in chemical refrigerator  Do not mouth pipette  Wash hands before leaving laboratory or after handling contaminated material
  • 29. Safe Practices These safe practices should be followed to ensure safe working conditions:  Do not use chipped or cracked glassware  When working with hazardous materials, have a second person nearby  Know emergency procedures  Keep the laboratory neat and clean  Use hazardous chemicals under a fume hood and biohazardous materials under a biosafety cabinet (BSC)  Decontaminate as needed  All procedures should be performed to minimize aerosol generation
  • 30. Know details/location of your vital Laboratory Information!
  • 31. Laboratory Information Each lab contains an Emergency Action Plan which is required for emergency situations. This is used to inform faculty/staff/students of the procedures to follow in the event of an emergency. This includes the following information:
  • 32. Laboratory Information The Chemical Hygiene Plan (CHP) is similar to the Hazard Communication Program, but this is specifically for laboratories with chemicals. This is to relay information regarding procedures, equipment, PPE, and work practices that are capable of protecting employees from health hazards. If you are working with acids, it would be nice to know what personal protective equipment is necessary and what health hazards are associated with it.
  • 33. Chemical Hygiene According to the laboratory standard, for those labs that have chemicals the following training topics must be addressed: Methods and observations that may be used to detect the presence or release of a hazardous chemicals. Physical and health hazards Measures employees can take to protect themselves from hazards Details of the CHP This training is covered in the Hazard Communication Training Presentation Trainin
  • 34. Chemical Hygiene Plan Methods and observations used to detect the presence or release of chemicals will be specific to your lab. A good indication of the presence of a chemical is to rely on your senses. Can you see it or smell it? Is a monitoring device needed to detect it? This can be important information if the chemical is an asphyxiant and pushes oxygen out of the room you are in.
  • 35. Chemical Hygiene Protection against chemicals is a combination of work procedures or practices, emergency procedures, and PPE. Some chemicals can only be handled under certain conditions. It is important to use proper handling procedures and practices as advised. The emergency procedures for chemical accidents is to first evacuate the area and then notify your supervisor, ISU campus police and EHS office if necessary.
  • 36. Chemical Hygiene Each lab that has chemicals will have a lab specific Chemical Hygiene Plan. This plan will detail the procedures and practices for your specific location. It is your responsibility to make sure that you review the CHP.
  • 37. Labeling It is important to know as much about a chemical as possible. The most dangerous substance is the one that has no label. Communicating information is essential in the science field.
  • 38. MSDS There are several constituents that are covered in the Laboratory Standard. Among these constituents includes labeling and the MSDS. Let’s look more closely at the content of the MSDS. MSDS
  • 39. MSDS In addition to labeling in a laboratory, the next most important type of communication regarding hazards is the MSDS. This is the acronym for Material Safety Data Sheet. This will communicate the information necessary regarding hazards associated with chemicals and also biological agents.
  • 40. MSDS So what is an MSDS? An MSDS is a document that relays vital information about certain chemicals and biological agents. MSDS Content Click here!
  • 41. Reading the MSDS Each component of the MSDS is broken down into sections. Each section goes into detail about what precautions to take and the characteristics of the substance. This is for both chemicals and biological agents. See the below links for example MSDSs. http://www.setonresourcecenter.com/MSDSs/comply1.htm
  • 42. Laboratory Information Why is an MSDS important? When you know characteristics about a substance it can aid in precautionary measure to take when using it. Also, if there is a spill either on a surface or on your skin, the MSDS can supply you with the information needed for first aid.
  • 43. MSDS The MSDS to every chemical in your lab must be available to you. It may be in a notebook in your labor available over the internet. Make sure you find the location of the MSDSs in your room. MSDS
  • 45. What is PPE? PPE is short for personal protective equipment. This is the equipment that is necessary to protect yourself from hazardous and biohazardous materials. PPE could be gloves, safety glasses, lab coat, shoe covers, respirator or any other item that could protect you from dangerous materials that you may encounter in the lab.
  • 46. When to use PPE Knowing what to use and when to use it is the key to properly protecting yourself. There could be situations that would be more of a risk and require more PPE than others. The next few slides will help you in determining what you should be using!
  • 47. When to use PPE Chemical usage poses a variety of hazards. They can be flammable, corrosive, even toxic just to name a few. Taking all precautions to avoid physical and/or health problems is the number one goal. You can never be too cautious!
  • 48. Chemicals & PPE When chemicals are being used there is always the possibility of splashing. The proper PPE to use when chemicals are involved would include: Safety glasses Gloves Respirator (depending on the chemical and the exposure duration) The Permissible Exposure Limit (PEL) for an 8 hr. shift can be obtained from the PEL/BEL reference guide. Contact EHS if questions arise.
  • 49. Proper Use of Personal Protective Equipment (PPE)
  • 50. Use of PPE Knowing how to properly use PPE can be the key to adequate protection. Not only do you want to make sure it is the proper size for you, but also make sure you are wearing it properly. If it is too big or too small, it is not right for you! Let your supervisor know if you need a different size.
  • 51. Use of PPE If you think you need a respirator talk with your supervisor or contact EHS (438-8325)
  • 52. Location & Availability of PPE Each Lab should have a designated area for the PPE that is used. The PPE should also be readily available when working with materials that require it. If the proper equipment is not available, you should not proceed with the work. Notify your supervisor when you need to order PPE. Give an advanced notice when possible due to shipping time. Always consider in advance what you will need when conducting research. PPE
  • 53. What is a Sharp?
  • 54. What is a “Sharp”? A sharp is defined as any instrument, tool, or item that has rigid, acute edges, protuberances or corners capable of cutting, piercing, ripping or puncturing such as syringes, blades, and broken glass. Items that have the potential for shattering or breaking are also considered sharps. http://www.ehrs.upenn.edu/training/bloodborne/bloodborne.html
  • 55. Safe Practices When using a sharp there is a risk of being cut by the object and possible infection occurring depending on what the sharp was used for. If hypodermic needles are used, special precautions must be taken to reduce the risk of a needlestick. After use of the needle do not recap, place directly in the sharp container.
  • 56. Disposal of Sharps All sharps must be placed into a rigid, puncture and leak-resistant container that is also impervious to moisture. The sharps container must be labeled either with “Biohazard” or “Infectious Waste”. Do not over fill the sharps container. http://www.ehrs.upenn.edu/training/bloodborne/bloodborne.html
  • 57. Disposal of Sharps When the sharps container is full it must be collected by the EHS office. A waste pick-up form can be completed and a collection can be scheduled. A waste pick-up form can be obtained from the EHS office (438-8325).
  • 58. BSL-1 Laboratory BIOHAZARD All Personal Protective Equipment shall be removed prior to leaving this work area. Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area prohibited in this work area. Name of infectious agent(s): __________________________________________________________________________________ __________________________________________________________________________________ Transmission route(s): __________________________________________________________________________________ Special requirements for entering this area: __________________________________________________________________________________ __________________________________________________________________________________ Emergency contacts Name: ________________________________ Title: ______________ Phone: _______________ ________________________________ _______________ _______________ ________________________________ _______________ _______________ EH&S office (M-F 8-5) 646-3327 (after hours) 911. __________________________________________________________________________________ *Biosafety Level 2 is similar to BSL-1 and is suitable for work involving agents of moderate potential hazard to personnel and the environment. It differs from BSL-1 in that 1.Lab personnel have specific training in handling pathogenic agents and directed to competent scientist; 2. Access to the lab is limited when work is being conducted: 3. Extreme precautions are taken with contaminated sharp items and 4. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological safety cabinets or other physical containment equipment New Mexico State University Date _______ Environmental Health & Safety Signs and Labeling
  • 59. Labeling Chemical labeling has been briefly touched on earlier in the presentation. One must remember that if any chemical is transferred to a secondary container, this container must be labeled. If the chemical will be used by the end of the work shift, then labeling is not necessary. Good science practices would encourage you to label all containers. So what is included on the Label? Click here
  • 60. Signs Signs are a way of communicating important information. It is a way to heighten awareness about hazards that exist. There may be signs on laboratory refrigerators reminding you that no food or drink can be stored in it. There may be radioactive or biological materials that could possibly be absorbed in food. There may also be signs which denote that PPE must be used.
  • 61. Signs Labs which use recombinant DNA BSL-1 Laboratory and infectious agents must have a sign posted on the outside of the BIOHAZARD door. Before someone enters the All Personal Protective Equipment shall be removed prior to leaving this work area. Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area prohibited in this work area. Name of infectious agent(s): lab, they will have the information __________________________________________________________________________________ __________________________________________________________________________________ Transmission route(s): __________________________________________________________________________________ they need to protect themselves. Special requirements for entering this area: __________________________________________________________________________________ __________________________________________________________________________________ Emergency contacts Name: ________________________________ Title: ______________ Phone: _______________ Always read the signs carefully so ________________________________ _______________ _______________ ________________________________ _______________ _______________ EH&S office (M-F 8-5) 646-3327 (after hours) 911. __________________________________________________________________________________ *Biosafety Level 2 is similar to BSL-1 and is suitable for work involving agents of moderate potential hazard to personnel and the environment. It differs from BSL-1 in that 1.Lab personnel have specific training in handling pathogenic agents and directed to competent scientist; you know what precautions to 2. Access to the lab is limited when work is being conducted: 3. Extreme precautions are taken with contaminated sharp items and 4. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological safety cabinets or other physical containment equipment New Mexico State University Date _______ Environmental Health & Safety take.
  • 62. Practices and Techniques Responsible practices and techniques are required when working with hazardous materials. This is one very important way to reduce accidents/injuries. Your supervisor will train you in the responsible use of hazardous materials specific to your laboratory.
  • 63. Safety Equipment The Primary Barriers
  • 64. Safety Equipment Certain equipment is necessary to achieve compliance and most importantly to provide adequate protection. The safety equipment that is needed is known as primary and secondary barriers. Let’s take a look at the difference in the barriers.
  • 65. Primary Barriers Primary barriers are referring to protective measures including engineering controls. This includes not only PPE that has already been covered, but it also includes safety cabinets, fume hoods, vaccines and autoclaves. It is important to know when this equipment is to be used and how to properly use it.
  • 66. Primary Barriers Let’s look at the following barriers: (Besides PPE) Fume hood Biological Safety Cabinet (BSC) Autoclave
  • 67. Fume Hood The fume hood is used with chemicals. The main function is to exhaust the vapors and gases that are generated in the hood to the outside. The hood is designed to minimize your exposure to airborne contaminants. This is not to be used with biohazardous materials.
  • 68. Fume Hood Use When using the fume hood you first need to make sure the exhaust blower is operating and air is entering the hood. Remember, do not put your face inside the hood! Minimize storage of chemicals in the hood Clean spills immediately Work with the sash at the proper operating level as indicated by the arrows
  • 69. Biological Safety Cabinet The biological safety cabinet (BSC) is used as a containment for infectious agents. The BSC has a HEPA filter in the exhaust system to protect the environment and yourself. The (HEPA) filter is a high efficiency particulate air filter. It is able to remove particles at a size of 0.3 µm with an efficiency of 99.97%. It is also able to remove both smaller and larger particles.
  • 70. BSC There are 3 classes of BSC that are used. The higher the risk group and biosafety level, the higher the class of cabinet that is used. If there is an infectious agent being used, whether it is used in research animals or cultured, it must be manipulated inside the BSC.
  • 71. PPE When using this containment device, remember to also use the proper personal protective equipment. The following PPE should be considered depending on the BSL that is required for the organism that you use. Gloves Lab Coat Shoe Covers Safety Glasses Respirator
  • 72. Use of PPE Always make sure that the BSC has been decontaminated both before and after use. Decontamination methods vary depending on the infectious agent being used. Once the decontamination of the cabinet is complete, place the waste in a biohazard bag for autoclave.
  • 73. Use of BSC Do not bring contaminated materials out of the cabinet until they have been surface decontaminated. If you are unable to decontaminate, place the material into a closed container to transfer it to the autoclave
  • 74. Work Practices for the BSC Remember to follow the work practices: Work in such a way that your face is above the front opening Wait for 1 minute after placing hands/arms inside the cabinet to stabilize the air flow Work at least 4 inches from the inside edge of the front of the grille
  • 75. Work Practices for the BSC Remember to place all materials as far back in the cabinet as possible Limit the storage within the cabinet
  • 76. Autoclave An autoclave is used to treat infectious material and recombinant DNA. As a standard for the University, all material of this nature must be autoclaved as a safety precaution. The autoclave is able to render the material as non-infectious.
  • 77. Autoclave The autoclave is able to reach a high temperature to sterilize the agent. It is important to know the standard operating procedures (SOP) for the autoclave. The SOP is located next to each autoclave. If the temperature or pressure is inadequate, the bag is overfilled, or the peak time is not long enough the material will not be properly decontaminated.
  • 78. Autoclave The autoclave is sensitive to time, temperature, pressure, water content, type of container being used and the type of waste being sterilized. When using the autoclave there are a few important point to remember in order for the process to be effective.
  • 79. Procedures The material being autoclaved must be placed inside an autoclave red bag or in a pan (Do not overfill the bag). Add ~250-500ml water to the bag or pan for steam to generate and properly circulate A spore strip or other approved tape must be placed on the outside of the bag or pan to verify that the heat treatment was successful. You will have to verify that a color change took place.
  • 80. Procedures The autoclave must reach a temperature of 121°C (250 °F) for at least ½ hour at a pressure of 15psi After successfully being autoclaved, the material can be placed in regular trash. Sharps must be incinerated. When the container is full, a pick up can be scheduled through EHS and from there Student Health Services will incinerate the sharps container for proper disposal
  • 81. Autoclave Log Each time the autoclave is used, the log must be completed by the individual using it. The log is to be located at the site of the autoclave. The information contained within the log is as follows: Date Generator Name (P.I.) Location Time the autoclave reached 121 °C Max Temp. Pressure Type of waste If spores or tape were used Weight Person using autoclave
  • 82. Autoclave Log The log is required by EPA and it must be maintained for 3 years by the department. The purpose of the log is to ensure that proper decontamination is taking place If a new log is needed at the autoclave, contact the biology department or EHS.
  • 84. Secondary Barriers Secondary barriers are the facility design and construction. These barriers are to provide protection for the individuals outside the lab, the community, and the environment.
  • 85. Secondary Barriers An example of a Secondary barrier would be a laboratory. It is separate from the classroom and has limited access. The animal room has limited access and is only available to authorized personnel. The autoclave is set away from the normal traffic. The handwashing facilities are located within the lab and not accessible by the outside students.
  • 87. Decontamination Decontamination is the removal or neutralization of toxic agents or the use of physical or chemical means to remove, inactivate, or destroy living organisms. This includes both sterilization and disinfection.
  • 88. Decontamination Decontamination is the responsibility of all laboratory workers. Failure to decontaminate can result in exposure to infectious agents which can cause great illness. Most decontamination can be done by chemicals. This technique is used only when autoclaving is not possible. Continue on to see what would be best for your lab.
  • 89. Chemicals There are a variety of chemicals that can be used as an effective method of decontamination. Depending on the agent being used, the method to use may vary along with the contact time. For most organisms, a 1:100 chlorine solution for 10-30 minutes is adequate. The Biosafety Manual has a list of sterilizers/ disinfectants that can be used.
  • 90. Autoclave The autoclave is the most effective method to use for decontamination purposes. As a general rule of thumb, autoclave all materials that are considered infectious agent, recombinant DNA, or resemble components of this nature. When in doubt, AUTOCLAVE! If a material is not capable of autoclave because of its size, material, or it is stationary, then rely on chemical disinfectant as a second option.
  • 92. SOP Spills and accidents can pose a serious health and safety threat. When a spill occurs, an aerosol can be created which can make the material several times more potent. The best measure to take in order to protect yourself is to be prepared. There should be standard operating procedures for this type of situation in your lab.
  • 93. What to Do? Being able to recognize the hazards, mitigate the spill, and notifying response authorities can be your best defense. The first response to a spill should be to evacuate the immediate area until the scope of the hazard has been addressed. Seek medical attention if necessary. Allow sufficient time for the aerosol to settle before considering entering the room. If you are responsible for clean up, proper training shall be addressed.
  • 94. How to Report When a spill occurs, it must be reported. Report to your supervisor all spills. If medical attention is needed, it is suggested to go to Student Health Services. All injuries that are a result of a spill must be reported to EHS.
  • 96. Waste Hazardous and biohazardous waste has special guidelines for proper disposal. It is important to properly dispose of waste to ensure human and environmental health. EPA regulates the waste that is generated at ISU.
  • 97. Waste Waste can be classified as either hazardous or biohazardous. Let’s take a closer look at the differences. Hazardous Waste- This is a waste which contains the characteristics of being any of the following: Toxic Corrosive Ignitable Flammable Oxidizer
  • 98. Waste A biohazardous waste is any waste that is considered infectious and/or because of its biological nature it can cause physical or health hazards in humans, animals, plants or the environment. This includes recombinant DNA and other genetically altered organisms and agents.
  • 99. Proper Disposal Waste that is considered biohazardous can be disposed of in regular trash once it has been rendered non-infectious. If a biohazard labeled bag is used, make sure it is either placed in a secondary bag or a completely new bag that is not red. http://www.lbl.gov/ehs/Medical/html/biohazardous.htm
  • 100. Proper Disposal Hazardous waste must be disposed of through the Environmental Health & Safety office. If you need to have hazardous waste picked up, contact EHS for a pick-up form. EHS 438-8325
  • 101. You have now completed the General Lab Safety Training presentation. If you have any questions please take the time to ask your supervisor or call EHS.