Cell culture involves growing cells under controlled conditions. It was first successfully done by Ross Harrison in 1907, though Roux had maintained embryonic chick cells in 1885. There are several key components of the culture conditions needed to keep cells alive, including a substrate that provides nutrients, growth factors, hormones, and a regulated environment with the proper pH, osmotic pressure, and temperature. When cells first proliferate from tissue in primary culture, they must later be subcultured and transferred to a new vessel with fresh medium to continue growing as they fill the available space. A cell line is a culture derived from a single cell or set of cells that can proliferate indefinitely under the right lab conditions.

1. Cell culture
DONE BY/ AHMED ELWAN & AHMED MAGDY

2. Introduction
Cell culture is the process by which
prokaryotic, eukaryotic or plant cells are
grown under controlled conditions.
Cell culture was first successfully
undertaken by Ross Harrison in 1907.
Roux in 1885 for the first time
maintained embryonic chick cells in a
cell culture.

3. Culture Conditions
Culture conditions vary widely for each cell type, but the artificial
environment in which the cells are cultured invariably consists of a
suitable vessel containing the following:
•A substrate or medium that supplies the essential nutrients(amino acids, carbohydrates,
vitamins, minerals).
•Growth factors.
•Hormones.
•Gases (O2, CO2).
•A regulated physico-chemical environment (pH, osmotic pressure, temperature).

4. Primary culture
Primary culture refers to the stage of the culture after the cells are
isolated from the tissue and proliferated under the appropriate
conditions until they occupy all of the available substrate. At this
stage, the cells have to be subcultured by transferring them to a
new vessel with fresh growth medium to provide more room for
continued growth.

5. Cell lines
A cell culture that is derived from one cell or set of cells of the same
type and in which under certain conditions the cells proliferate
indefinitely in the laboratory.

6. Good Bye