Carbohydrates are compounds made of carbon, hydrogen, and oxygen. There are three main types: monosaccharides like glucose are simple sugars; disaccharides like sucrose are two monosaccharides bonded together; and polysaccharides like starch and glycogen are long chains of monosaccharides. Carbohydrates provide the body with its primary source of energy and are found naturally in foods like grains, fruits, vegetables, and sugars. A deficiency in carbohydrates can lead to hypoglycemia, ketosis, acidosis, weight gain, and decreased energy levels. Carbohydrates can be tested for using Benedict's solution, which changes color depending on the presence of reducing sugars.
Polysaccharide introduction, example, structure, starch, cellulose, chitin those structure and important functions and their presence in plants and animals, polysaccharide types based on functions and their composition , functions of polysaccharides , important images for relevant polysaccharides types, polysaccharide role in plants and animal cells. Starch - structure and functions, cellulose structure and functions, chitin - structure and functions
Introduction and defination
Classification
Reducing sugars
Non-reducing sugars
General properties
Common disaccharides
1) sucrose
Origin
Structure
Properties
Function
Polysaccharide introduction, example, structure, starch, cellulose, chitin those structure and important functions and their presence in plants and animals, polysaccharide types based on functions and their composition , functions of polysaccharides , important images for relevant polysaccharides types, polysaccharide role in plants and animal cells. Starch - structure and functions, cellulose structure and functions, chitin - structure and functions
Introduction and defination
Classification
Reducing sugars
Non-reducing sugars
General properties
Common disaccharides
1) sucrose
Origin
Structure
Properties
Function
The topic is about carbohydreates.
This lecture will cover an introduction to carbohydrates, its classification and exmaples. it will also cover the difference between glycemic index, difference between complex vs simple carb and also what are the fuctions of carbohydrates. this content will be helpful for all categories of students. 2014 study published in JAMA and youtube sources helps me in preparing lecture.
Glucose tolerance test- Indications, contraindications, preparation of a patient, precautions, types of GTT, normal curve, diabetic curve, renal glycosuria, lag curve, Criteria for diagnosis of DM
The topic is about carbohydreates.
This lecture will cover an introduction to carbohydrates, its classification and exmaples. it will also cover the difference between glycemic index, difference between complex vs simple carb and also what are the fuctions of carbohydrates. this content will be helpful for all categories of students. 2014 study published in JAMA and youtube sources helps me in preparing lecture.
Glucose tolerance test- Indications, contraindications, preparation of a patient, precautions, types of GTT, normal curve, diabetic curve, renal glycosuria, lag curve, Criteria for diagnosis of DM
Every day we are eating something, so these food products giving energy to human body.
in that CHO is the primary source of energy. Carbohydrates are one of the three main classes of foods and a source of energy. Carbohydrates are mainly sugars and starches that the body breaks down into glucose (a simple sugar that the body can use to feed its cells).
This Slide share includes Carbohydrate and its Nutrition. It includes introduction, classification, digestion and absorption, sources, RDA and effects of excess and limited use of carbs and fibre and its health effects.
At the end of this lecture, the students should be able to:-
1. Differentiate simple carbohydrates and complex carbohydrate
2. Describe the function of carbohydrates in the body
3. Demonstrate knowledge of health with carbohydrates
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
2. What are Carbohydrates?
• Carbohydrates (also called saccharides) are molecular
compounds made from just three elements: carbon,
hydrogen and oxygen.
3. Types of Carbohydrates…
• Monosaccharides:
They are the simplest carbohydrates and are often called simple
sugars. They are the building blocks from which all bigger
carbohydrates are made. (For example: Glucose, fructose, ribose,
etc.)
• Disaccharides:
They are sugars composed of 2 monosaccharides. (For example:
Sucrose, maltose, lactose, etc.)
• Polysaccharides:
They are complex carbohydrates, composed of 10 to up to
several thousand monosaccharides arranged in chains. (For
example: Starch, Cellulose, Glycogen, etc.)
4. Monosaccharides
• Monosaccharides are the
simplest carbohydrates.
Thy are the building blocks
from which all other
complex carbohydrates are
made. Glucose is a simple
sugar. It is made up of 6
Carbon atoms joined in a
ring, with the H2 and O2
atoms pointing out from
and into the ring. Its
formula is C6H12O6. Simple
sugars are soluble in water
and taste sweet.
The structure of Glucose.
5. Function of monosaccharides...
• Monosaccharides are an energy source; most of them provide
about 4 Calories (kilocalories) per gram, just like other
carbohydrates.
• Glucose is the main fuel for the body cells and is used for
respiration.
• Fructose participates in metabolism.
• Ribose is part of deoxyribonucleic acid (DNA) in the
chromosomes.
• However, monosaccharides are non-essential nutrients,
which means your body can produce all of those it needs
for proper functioning from other nutrients, so you do not
need to get them from food.
7. Disaccharides
• Disaccharides are sugars
composed of 2
monosaccharides.
Disaccharides, along with
monosaccharides, are called
simple carbohydrates. A
disaccharide molecule is
formed by 2 monosaccharides,
joined by a glycosidic bond. A
glycosidic bond is a covalent
bond which bonds a
carbohydrate molecule to
another molecule. The formula
of Sucrose is C12H22O11. They
are soluble in water and taste
sweet.
Structure of Sucrose
8. Function of Disaccharides...
• Disaccharides are an energy source; most of them provide
about 4 Calories (kilocalories) per gram, just like other
carbohydrates.
• Again, disaccharides are non-essential nutrients,
which means they are not necessary for health or life
of human beings.
10. Polysaccharides
• If many simple sugars join together,
a very large molecule called a
polysaccharide is made. Some
polysaccharide molecules contain
thousands of sugar molecules
joined together in a long chain. The
cellulose of plant cell walls is a
polysaccharide and so is starch.
Animal cells contain
polysaccharides known as
glycogen. Most polysaccharides are
insoluble and are not sweet. Starch
is the most popular polysaccharide.
Its formula is (C6H10O5)n, where n
is the number of glucose molecules
present.
11. Types and functions of
Polysaccharides...
• Starch: it is an energy source obtained from plants.
• Cellulose: it is a structural polysaccharide in plants; when
consumed, it acts as a dietary fiber.
• Glycogen: it is a storage form of glucose in the human liver
and muscles.
• They provide carbon atoms for the synthesis of fats, proteins
and other substances in your body.
• Non-digestible polysaccharides or dietary fiber, such as
cellulose, promote the passage of food through the gut and thus
help maintain bowel regularity.
• Some non-digestible polysaccharides, such as inulin, may also
promote the growth of beneficial intestinal bacteria.
13. General functions of
Carbohydrates...
• Carbohydrate functions as primary sources of energy.
• It functions as storage of food.
• It functions as framework in body.
• It functions as anticoagulant.
• It functions as antigen.
• It functions as hormones.
14. Sources of carbohydrates
in food...
• Starch is found in
cereals, corn-flour,
potatoes, bread, pasta,
flour, beans, rice, etc.
• Sugar is found in fruit,
cakes, desserts,
vegetables, honey, etc. Sugary foods
17. Hypoglycemia
• Hypoglycemia, also known as low blood sugar or low blood
glucose, occurs when the glucose levels in the blood drop below
normal. While hypoglycemia is often associated with diabetes, it
can be caused by a lack of carbohydrates in healthy people.
Symptoms of hypoglycemia include tiredness, weakness, light-
headedness, confusion and hunger. Carbs are the main source of
glucose because they are broken down into simple sugars during
digestion and enter the cells with the help of insulin, providing
energy. Eating a small amount of carbs will quickly treat
hypoglycemia.
18. Ketosis
• Eating less than 130 grams of carbohydrates a day can cause a
buildup of ketones, which are partially broken-down fats in the
blood. This condition is known as ketosis, according to the Weight-
Control Information Network, a service of the National Institute of
Diabetes and Digestive and Kidney Diseases. When your body
doesn’t have enough glucose for energy, it breaks down stored fat,
producing ketones. Mild ketosis can cause mental fatigue, bad
breath, nausea and a headache, but severe ketosis can lead to painful
swelling of the joints and kidney stones. Aim for 225 to 325 grams of
carbohydrates a day to prevent ketosis and other health problems.
19. Acidosis
• In carbohydrate starvation, there is shift from glycolysis
(breakdown of glucose) to lipolysis (breakdown of lipids)
and keto-genesis for energy needs. The resultant
production of keto-acids increases acidity in the blood
and other body tissues. These changes in the pH of
arterial blood outside 7.35 pH - 7.45 pH result in
irreversible cell damage.
20. Weight gain.
• Yes! It is true. While low-carb diets are often used for weight loss,
severely restricting carbs can actually have the opposite effect by
making you feel hungrier than normal. When you’re feeling
ravenous, you’re more likely to reach for fattening goodies rather
than carrot sticks, leading to weight gain over time. Studies show
that people following a low-carb diet for two years lost an average of
9 pounds, which is similar to the amount lost on higher-carb diets,
MayoClinic.com reports. Consuming healthy, low-calorie
carbohydrates may actually help weight loss because they’re high in
fiber, which fills you up without filling you out.
21. Along with those...
• Fatigue and decreased energy levels.
• Muscle wasting
• Dehydration and reduced body secretions
• Loss of sodium
• Weakened immune system
• Constipation
• Mood swing
22. Test for Carbohydrates...
• We can test for the presence of sugars by adding
Benedict’s solution to food, and heating it. If the
food contains reducing sugars such as glucose or
maltose, then a brick – red color will form. The
mixture gradually changes from blue, through
green, yellow and orange, and then finally, brick
red. If there is no reducing sugar, then the
Benedict’s solution remains blue.
23.
24. Conclusion
• So what we have covered in this PPT are the types of
carbohydrates and their structures and functions, the
general functions of carbohydrates, the different sources
of carbohydrates and what happens if you have too little
carbohydrates. Under this, we learned about
hypoglycemia, ketosis and weight gain. We also learned
how to test whether carbohydrates are present or no using
Benedict’s solution.
Immunological properties to blood – antigen.
FSH (Follicular Stimulating Hormone which takes part in ovulation in females) and LH (Leutinizing Hormone) are glycoprotein and help in reproductive processes.
As there is loss of fluids from the body due to ketosis, a state of chronic dehydration is reached. This results in reduction in mucus secretion, dry eyes and compromised mucus production in tear glands, salivary glands, sinuses, airways, and gastrointestinal tract.
Excess of ketone in the blood leads to fluid loss and excretion of sodium ions(Na+) from the body. This may lead to muscle cramps, exhaustion and lassitude.
With increased fluid loss and degradation, vitamin C loss from the body is increased. Adding to this, the chronically dehydrated condition leads to weakened immune system and susceptibility to infections.
Dietary carbohydrate exclusion causes the brain to stop regulating serotonin hormone. Low serotonin supply causes mood swing and depression.