Electrophoresis: Electrophoresis is separation technique based on movement of charge particle in an electric field. Movement of charge particles can be determined by following formula-- V= Eq/f Where, V= Velocity of the charged particle; E= electric field of the molecule; q= Net charge of the molecule; and f= Frictional co-efficient of the molecule Types of electrophoresis: 1. Agarose gel electrophoresis ; 2. Poly-acryl amide gel electrophoresis [PAGE]; 3. Sodium do-decyl sulphate Poly- acrylamide gel electrophoresis [SDS-PAGE] ; 4. Two dimensional –Poly-acrylamide gel electrophoresis [2D-PAGE]; 5. Pulse field gel electrophoresis [PFGE]; 6. Capillary gel electrophoresis [CGE]; and 7. Disc electrophoresis for Protein. Application of electrophoresis: 1. Estimation of the DNA molecule.[ Agarose , PAGE ] 2. Analysis of PCR product. [ Agarose ] 3. Separation of restricted genomic DNA and RNA. [Agarose and PAGE respectively] 4. Conformation of newly isolated DNA .[Agarose] 5. Separation of most small fragments of DNA. [PAGE] 6. In forensic science.[Agarose , PAGE, SDS-PAGE, 2D PAGE ,Capillary gel electrophoresis , PFGE] 8. In determining molecular wt. of protein.[SDS-PAGE].etc

1. ELECTROPHORESIS
Presented By,
Dipankar Saikia,
M.Sc. in ZOOLOGY,
UID-14PGZ10,
DEPTT OF ZOOLOGY DARRANG COLLEGE

2. WHAT IS ELECTROPHORESIS
Electrophoresis is separation technique
based on movement of charge particle in an
electric field.
Movement of charge particles can be
determined by following formula---
Where,
V= Velocity of the charged particle;
E= electric field of the molecule;
q= Net charge of the molecule; and
f= Frictional co-efficient of the molecule .
V=E q ∕ f

3. TYPES OF ELECTROPHORESIS
1. Agarose gel electrophoresis ;
2. Poly-acryl amide gel electrophoresis[PAGE];
3. Sodium do-decyl sulphate Poly- acrylamide gel
electrophoresis[SDS-PAGE] ;
4. Two dimensional –Poly-acrylamide gel
electrophoresis[2D-PAGE];
5. Pulse field gel electrophoresis[PFGE];
6. Capillary gel electrophoresis[CGE]; and
7. Disc electrophoresis for Protein.

4. AGAROSE GEL ELECTROPHORESIS
PRINCIPLE:
1.It is a horizontal gel electrophoresis.
2.Molecules are separated on the basis of size ,
shape and charge.
3.To visualize movement of particle loading dye is
added.

5. Fig: Agarose gel electrophoresis.
source: https://www.bing.com/image

6. POLY-ACRYLAMIDE GEL ELECTROPHORESIS
[PAGE]
PRINCIPLE:
1.It is a vertical gel electrophoresis.
2.Negatively charged (-ve ) DNA and RNA molecule
can be separated.
3.Molecules are separated on the basis of size ,
shapes and molecular weight.

7. Fig: Poly- acryl amide gel electrophoresis. [PAGE].
Source: http://www.google.com/image

8. SODIUM DO-DECYL –POLYACRYL AMIDE
GEL ELECTROPHOERESIS[SDS-PAGE]
PRINCIPLE:
1.Negatively charged Protein sample can be
separated.
2.SDS detergent is used to give negative charge to the
positively charged protein molecule.
3.Hydrocarbon tail of the SDS molecule is bind with
the hydrophobic region of the protein molecule.

9. Fig:- SDS PAG
source: https://www.bing.com/image
E

10. TWO DIMENSIONAL POLY-ACRYL GEL
ELECTROPHORESIS[2D-PAGE [2D-PAGE]
PRINCIPLE:
1. It is a separation technique for Protein.
2. The proteins which have same molecular
weight and iso-electric point can be separate.
3. The first dimension of it is vertical and second
dimension is normal PAGE.

11. Fig:_2-dimensional Poly-acryl amide Gel Electrophoresis (2D-
PAGE) .
Source: http://www.google.com/image

12. PULSE FIELD GEL ELECTROPHORESIS
[PFGE]
PRINCIPLE:
1. Negatively charged large DNA molecule can be
separated.
2. Above the length of 30-50 Kb molecules can be
separated.
3. Here field direction is different.

13. Fig-Pulse field gel Electrophoresis.
Source: http://www.google.com/image

14. CAPILLARY GEL
ELECTROPHORESIS[CGE]
PRINCIPLE:
1.It vertical electrophoresis.
2.Besed on shape, size, molecular weight, and
electric charge.

15. Fig:- Capillary Gel Electrophores
Source: http://www.google.com/image
is.

16. DISCONTINUOUS[DISC]
ELECTROPHRESIS
PRINCIPLE:
1.It is a protein electrophoresis.
2.It solve the problem of aggregation and precipitation
of protein.
3. Discontinuity is based on –
*the gel structure;
*the pH value and ionic streangth of the buffer;
*the nature of the ion in the and the electrode
buffer.

17. Fig: Discontinuous gel electrophoresis.
source: https://www.bing.com/image

18. APPLICATION OF ELECTROPHORESIS
1. Estimation of the DNA molecule.[ Agarose , PAGE ]
2. Analysis of PCR product.[ Agarose ]
3. Separation of restricted genomic DNA and RNA.[Agarose
and PAGE respectively]
4. Conformation of newly isolated DNA .[Agarose]
5. Separation of most small fragments of DNA.[PAGE]
6. In forensic science.[Agarose , PAGE, SDS-PAGE, 2D PAGE
,Capillary gel electrophoresis , PFGE]
8. In determining molecular wt. of protein.[SDS-PAGE].etc

19. REFERENCES
Dubey R.C., 2013 a text book of Bio-
technology.
Reddy P. R.2012 Gel electrophoresis and its
application(review paper)

20. THANK YOU