Quality assurance in blood banking involves implementing quality control checks at various stages of the blood collection and processing process. This includes donor screening, blood collection following aseptic techniques, proper storage and labeling of blood components, sterility testing, equipment calibration, and testing blood components for key attributes like volume and factors. Regular internal and external quality audits help ensure standards are maintained.
This document provides guidelines for routine venipuncture and specimen handling. It describes the venipuncture procedure, including patient preparation, vein selection, order of draw, labeling samples, and troubleshooting incomplete collections. It also lists the additive, function, and uses of various colored collection tube types, such as red top for chemistries, gold top for serum separation, and purple top for complete blood counts. Complications like hematomas are addressed.
This document provides information about the objectives and theory of phlebotomy. It discusses what phlebotomy is, the roles and responsibilities of phlebotomists, and related anatomy and physiology. It also covers important topics like professionalism, safety, equipment used, and procedures for collecting blood. Phlebotomists must properly identify patients, take safety precautions, position the patient, locate a vein, and collect blood samples while maintaining patient comfort and confidentiality.
This document provides information and guidelines on blood specimen collection through venipuncture. It discusses identifying patients, preparing the collection site and equipment, performing the venipuncture using proper technique, and handling and processing the specimens. Specific topics covered include selecting appropriate vein sites, using tourniquets and antiseptics, avoiding complications, and processing and storing specimens according to their test requirements. The goal is to provide high quality specimens by following standard procedures.
Microbiology of the GIT , Abdallh dwayat.pdf8y4xzv9rqd
1. The document discusses Gram-negative rods related to the enteric tract, including E. coli, Shigella, Vibrio cholerae, and others.
2. It covers the major clinical manifestations of enteric infections like diarrhea, vomiting, and abdominal pain.
3. Laboratory diagnosis of enteric bacteria involves using differential and selective media like MacConkey agar and Eosin methylene blue agar to differentiate lactose fermenters from non-fermenters. Tests like triple sugar iron agar also help identify bacteria.
This document discusses microscopic examination of urine sediment. It describes how to prepare and examine a urine sample under the microscope. The deposits seen are divided into organic and inorganic categories. Organic deposits include cells, casts, crystals and other abnormal elements. Common cell types like red blood cells, white blood cells and epithelial cells are described. Different types of casts are defined along with their clinical significance. Various crystals that may be present in normal and abnormal urine are also outlined.
The document describes the organizational divisions and sections within a clinical laboratory. It outlines the departments of pathology, including anatomical pathology, cytology, histology, cytogenetics, and clinical pathology. The clinical pathology department contains sections for hematology, chemistry, blood bank, serology, microbiology, and urinalysis. Each section is described in terms of its functions, common tests performed, and specimen requirements. The document also discusses laboratory personnel roles and requirements.
The document provides instructions for drawing blood from donors. It states that donors must be healthy and comfortable, and the skin where blood will be drawn must be thoroughly cleaned. It also notes that blood is drawn into bottles or bags containing an anticoagulant solution to prevent clotting, and several safety and quality control procedures are followed before and after drawing blood.
Quality assurance in blood banking involves implementing quality control checks at various stages of the blood collection and processing process. This includes donor screening, blood collection following aseptic techniques, proper storage and labeling of blood components, sterility testing, equipment calibration, and testing blood components for key attributes like volume and factors. Regular internal and external quality audits help ensure standards are maintained.
This document provides guidelines for routine venipuncture and specimen handling. It describes the venipuncture procedure, including patient preparation, vein selection, order of draw, labeling samples, and troubleshooting incomplete collections. It also lists the additive, function, and uses of various colored collection tube types, such as red top for chemistries, gold top for serum separation, and purple top for complete blood counts. Complications like hematomas are addressed.
This document provides information about the objectives and theory of phlebotomy. It discusses what phlebotomy is, the roles and responsibilities of phlebotomists, and related anatomy and physiology. It also covers important topics like professionalism, safety, equipment used, and procedures for collecting blood. Phlebotomists must properly identify patients, take safety precautions, position the patient, locate a vein, and collect blood samples while maintaining patient comfort and confidentiality.
This document provides information and guidelines on blood specimen collection through venipuncture. It discusses identifying patients, preparing the collection site and equipment, performing the venipuncture using proper technique, and handling and processing the specimens. Specific topics covered include selecting appropriate vein sites, using tourniquets and antiseptics, avoiding complications, and processing and storing specimens according to their test requirements. The goal is to provide high quality specimens by following standard procedures.
Microbiology of the GIT , Abdallh dwayat.pdf8y4xzv9rqd
1. The document discusses Gram-negative rods related to the enteric tract, including E. coli, Shigella, Vibrio cholerae, and others.
2. It covers the major clinical manifestations of enteric infections like diarrhea, vomiting, and abdominal pain.
3. Laboratory diagnosis of enteric bacteria involves using differential and selective media like MacConkey agar and Eosin methylene blue agar to differentiate lactose fermenters from non-fermenters. Tests like triple sugar iron agar also help identify bacteria.
This document discusses microscopic examination of urine sediment. It describes how to prepare and examine a urine sample under the microscope. The deposits seen are divided into organic and inorganic categories. Organic deposits include cells, casts, crystals and other abnormal elements. Common cell types like red blood cells, white blood cells and epithelial cells are described. Different types of casts are defined along with their clinical significance. Various crystals that may be present in normal and abnormal urine are also outlined.
The document describes the organizational divisions and sections within a clinical laboratory. It outlines the departments of pathology, including anatomical pathology, cytology, histology, cytogenetics, and clinical pathology. The clinical pathology department contains sections for hematology, chemistry, blood bank, serology, microbiology, and urinalysis. Each section is described in terms of its functions, common tests performed, and specimen requirements. The document also discusses laboratory personnel roles and requirements.
The document provides instructions for drawing blood from donors. It states that donors must be healthy and comfortable, and the skin where blood will be drawn must be thoroughly cleaned. It also notes that blood is drawn into bottles or bags containing an anticoagulant solution to prevent clotting, and several safety and quality control procedures are followed before and after drawing blood.
Blood can be separated into components like red blood cells, platelets, cryoprecipitate, and frozen plasma which are useful for different medical purposes. Whole blood is rarely used now due to the risk of volume overload. The Coombs test, also known as the antiglobulin test, detects the presence of antibodies and can be performed directly on a patient's red blood cells or indirectly by incubating their serum with donor red blood cells. A positive result in either test indicates the presence of antibodies.
The document provides an overview of phlebotomy, including the roles and responsibilities of phlebotomists, safety precautions for handling blood and needles, anatomy related to blood collection, equipment used in blood collection, and proper procedures for collecting blood samples from patients. It describes the importance of identifying patients correctly, taking standard precautions, and positioning patients comfortably to facilitate successful blood draws with minimal risk of complications or exposure to blood-borne pathogens.
This document discusses quality assurance in blood banking. It outlines that quality is an ongoing process involving planning, doing, checking, and acting in a continuous cycle. Quality control ensures processes, procedures, and products meet requirements through consistency checks. Blood banking requires separate areas for donor selection, collection, processing, storage, and laboratory and auxiliary facilities. Quality requirements involve quality control testing, audits, personnel organization, premises/equipment/materials, documentation, processing, complaints/recalls, and investigating errors/accidents.
Vacuum collection tubes are used to draw blood samples. They contain a partial vacuum and various additives depending on the required test. Common additives include anticoagulants to prevent clotting for tests like CBCs, or gels and clot activators for tests needing serum. It is important to fill each tube completely to ensure proper mixing of blood and additives for accurate results. Tube stoppers are color coded to indicate their additive for tests like coagulation, blood banking, or chemistries. Needles must be disposed of properly in sharps containers after use.
Routine lab tests provide important health information. Some common tests include:
- A complete blood count evaluates red blood cells, white blood cells, and platelets. It screens for anemia and infection.
- Blood chemistries analyze chemical substances like electrolytes, kidney and liver enzymes, lipid profiles, and blood glucose. This provides insights into organ function.
- Additional tests like coagulation profiles and hormonal assays evaluate risks of bleeding, metabolic conditions, and pregnancy.
Together, routine lab tests form a baseline of a person's health and can detect early signs of disease if values are outside normal ranges.
A blood transfusion is the administration of blood or blood components into a patient's circulatory system through an intravenous line. The main purposes are to restore blood volume, replace clotting factors, improve oxygen carrying capacity, and restore depleted blood elements. Indications for transfusion include significant blood loss, anemia, low platelet count, and coagulation disorders. Whole blood contains red blood cells, white blood cells, plasma, and platelets. Other blood products administered include packed red blood cells, platelets, fresh frozen plasma, and albumin. Nurses monitor patients during and after transfusion for signs of reactions and ensure documentation of the procedure.
Blood collection tubes also know as vacutainer tubes or phlebotomy tubes, are sterile tubes use to collect and transport blood specimens for medical testing or analysis. They are design to draw and store a specific volume of blood, usually ranging from 2-10 milliliters, depending on the type of tube used and the intended application.
Washed red blood cell suspensions are prepared to remove plasma proteins that could interfere with antigen-antibody reactions during blood typing tests. The red blood cells are separated from whole blood via centrifugation and washed with saline to remove plasma. This helps remove soluble antigens, interfering proteins, and substances that could cause false positive reactions. The washed red blood cells are then suspended in saline at a 3-5% concentration for use in blood typing tests.
Anticoagulant and Blood Collecting Tubes MushahidAli23
This document summarizes different types of blood collection tubes containing various anticoagulants or clot activators. It describes the color-coded tube caps and additive in each tube and their main uses. Common tubes discussed include purple top for hematology tests, red top for serum tests, light blue top for coagulation tests, and green top for tests requiring plasma. The document provides details on anticoagulants like EDTA, sodium citrate, and oxalates, and how they work to preserve blood components for various laboratory analyses.
This document provides information on different types of blood collection tubes including their purposes and contents. It discusses EDTA tubes which are used for complete blood counts and DNA isolation, citrate tubes which contain citrate and are used for prothrombin time tests, heparin tubes coated with heparin for karyotyping, gel tubes which separate plasma or serum from cells, plain red vials which separate serum after clot formation, and grey glucose vials containing sodium fluoride and potassium oxalate to prevent glucose consumption during processing. It emphasizes the importance of inverting tubes to mix anticoagulants and lists the recommended order of blood draw.
The document discusses various components of a basic blood test. It provides details on the normal composition and functions of blood, as well as procedures for blood specimen collection. It also explains the clinical implications and reference ranges for various components analyzed in a complete blood count test, including red blood cells, white blood cells, platelets, hematocrit, hemoglobin, and sedimentation rate.
This document discusses the common types of blood collection test tubes used in hospitals. It explains that red top tubes contain silica clot activators and are used for serum tests like cholesterol and liver enzymes. Purple top tubes contain anticoagulants and are used for blood and fluid cultures. Blue top tubes contain sodium citrate to prevent clotting and are used for coagulation tests. Green top tubes contain heparin and are used for chemistry tests. Lavender top tubes contain EDTA and are used for hematology tests. Gray top tubes contain sodium fluoride and potassium oxalate to prevent glucose breakdown and are used for chemistry tests like glucose. The document stresses that the proper test tube must be chosen according to the diagnostic
The clinical lab provides diagnostic test data to aid in the detection, diagnosis and treatment of disease. The lab is responsible for correct identification, collection and processing of patient specimens, accurate performance of testing, timely reporting of results, and communication with healthcare professionals. There are six main steps in how a sample flows through the lab: 1) test is ordered, 2) sample is collected, 3) sample is delivered to the lab, 4) sample is processed, 5) sample is analyzed, and 6) results are reported. Common specimen types include blood, urine, body fluids, sputum, stool, and tissue samples.
The document discusses different methods of blood collection including capillary, venous, and arterial blood. It describes the equipment and procedures used to collect blood via these methods and the tests that each type is suited for. The document also outlines the various anticoagulants used in blood collection tubes, including EDTA, citrate, heparin, oxalate, and their mechanisms and uses. Finally, it addresses the order that tubes should be drawn in and storage of blood in blood banks.
Blood Transfusion: Procedure and ComplicationsShraddha Madghe
Blood transfusion is a medical procedure where blood or blood components are transferred from one person into the circulatory system of another. There are several types of blood transfusion including allogeneic where the donor and recipient are different people, and autogenic where a person receives their own previously donated blood. The blood transfusion procedure involves verifying the blood type, obtaining consent, administering the transfusion, and monitoring for any complications. Potential complications include transfusion reactions if the blood is mismatched, infections transmitted through the blood, and issues that can arise from using faulty techniques or giving massive amounts of blood.
Urine is commonly used for diagnostic testing and monitoring disease. Proper collection and preservation of urine specimens is important to avoid preanalytical errors. There are various urine collection methods depending on the test and patient, including random, first morning, and timed collections. Preservatives like boric acid and hydrochloric acid are often used to maintain specimen stability, though some tests require no preservative. Careful instruction of patients and labeling of specimens is necessary when collecting 24-hour urine samples.
Automatic And Semi Automatic Analyser Biochemistry MdShamsTabrez4
The AutoAnalyzer is an automated analyzer that uses continuous flow analysis, invented in 1957. It has five main parts: the sample pump, mixing chamber, incubator bath, and detector. AutoAnalyzers were mainly used for routine medical and industrial analyses to increase throughput. While still used for some applications, most instruments are now used industrially and for environmental work.
This document describes different types of blood collection tubes used in phlebotomy and their purposes. It discusses tubes containing various anticoagulants such as EDTA, sodium citrate, and sodium heparin that are used for hematology and coagulation tests. It also describes serum separator tubes containing silica and gel that are used for biochemical and other tests requiring separated serum. The document provides examples of common tests performed using each type of tube.
The document provides information on the various colored blood collection tubes used in laboratories, including their common tests and proper usage. It discusses the purple, pink, blue, yellow, grey, red, dark green, and light green tubes, listing their additives, most common tests, and tips for proper collection techniques. While laboratory preferences may vary, this provides guidance on matching tests to the correct anticoagulated tube.
This document provides information about different colored blood collection tubes and their uses. It discusses the purposes and additives of purple, pink, blue, yellow, grey, and red tubes. The purple, pink, and blue tubes contain anticoagulants and are used for hematology and coagulation tests. The yellow, grey, and red tubes are used for biochemical and other serum-based tests. It provides examples of common tests performed using each tube type and tips for proper sample collection and handling.
Blood can be separated into components like red blood cells, platelets, cryoprecipitate, and frozen plasma which are useful for different medical purposes. Whole blood is rarely used now due to the risk of volume overload. The Coombs test, also known as the antiglobulin test, detects the presence of antibodies and can be performed directly on a patient's red blood cells or indirectly by incubating their serum with donor red blood cells. A positive result in either test indicates the presence of antibodies.
The document provides an overview of phlebotomy, including the roles and responsibilities of phlebotomists, safety precautions for handling blood and needles, anatomy related to blood collection, equipment used in blood collection, and proper procedures for collecting blood samples from patients. It describes the importance of identifying patients correctly, taking standard precautions, and positioning patients comfortably to facilitate successful blood draws with minimal risk of complications or exposure to blood-borne pathogens.
This document discusses quality assurance in blood banking. It outlines that quality is an ongoing process involving planning, doing, checking, and acting in a continuous cycle. Quality control ensures processes, procedures, and products meet requirements through consistency checks. Blood banking requires separate areas for donor selection, collection, processing, storage, and laboratory and auxiliary facilities. Quality requirements involve quality control testing, audits, personnel organization, premises/equipment/materials, documentation, processing, complaints/recalls, and investigating errors/accidents.
Vacuum collection tubes are used to draw blood samples. They contain a partial vacuum and various additives depending on the required test. Common additives include anticoagulants to prevent clotting for tests like CBCs, or gels and clot activators for tests needing serum. It is important to fill each tube completely to ensure proper mixing of blood and additives for accurate results. Tube stoppers are color coded to indicate their additive for tests like coagulation, blood banking, or chemistries. Needles must be disposed of properly in sharps containers after use.
Routine lab tests provide important health information. Some common tests include:
- A complete blood count evaluates red blood cells, white blood cells, and platelets. It screens for anemia and infection.
- Blood chemistries analyze chemical substances like electrolytes, kidney and liver enzymes, lipid profiles, and blood glucose. This provides insights into organ function.
- Additional tests like coagulation profiles and hormonal assays evaluate risks of bleeding, metabolic conditions, and pregnancy.
Together, routine lab tests form a baseline of a person's health and can detect early signs of disease if values are outside normal ranges.
A blood transfusion is the administration of blood or blood components into a patient's circulatory system through an intravenous line. The main purposes are to restore blood volume, replace clotting factors, improve oxygen carrying capacity, and restore depleted blood elements. Indications for transfusion include significant blood loss, anemia, low platelet count, and coagulation disorders. Whole blood contains red blood cells, white blood cells, plasma, and platelets. Other blood products administered include packed red blood cells, platelets, fresh frozen plasma, and albumin. Nurses monitor patients during and after transfusion for signs of reactions and ensure documentation of the procedure.
Blood collection tubes also know as vacutainer tubes or phlebotomy tubes, are sterile tubes use to collect and transport blood specimens for medical testing or analysis. They are design to draw and store a specific volume of blood, usually ranging from 2-10 milliliters, depending on the type of tube used and the intended application.
Washed red blood cell suspensions are prepared to remove plasma proteins that could interfere with antigen-antibody reactions during blood typing tests. The red blood cells are separated from whole blood via centrifugation and washed with saline to remove plasma. This helps remove soluble antigens, interfering proteins, and substances that could cause false positive reactions. The washed red blood cells are then suspended in saline at a 3-5% concentration for use in blood typing tests.
Anticoagulant and Blood Collecting Tubes MushahidAli23
This document summarizes different types of blood collection tubes containing various anticoagulants or clot activators. It describes the color-coded tube caps and additive in each tube and their main uses. Common tubes discussed include purple top for hematology tests, red top for serum tests, light blue top for coagulation tests, and green top for tests requiring plasma. The document provides details on anticoagulants like EDTA, sodium citrate, and oxalates, and how they work to preserve blood components for various laboratory analyses.
This document provides information on different types of blood collection tubes including their purposes and contents. It discusses EDTA tubes which are used for complete blood counts and DNA isolation, citrate tubes which contain citrate and are used for prothrombin time tests, heparin tubes coated with heparin for karyotyping, gel tubes which separate plasma or serum from cells, plain red vials which separate serum after clot formation, and grey glucose vials containing sodium fluoride and potassium oxalate to prevent glucose consumption during processing. It emphasizes the importance of inverting tubes to mix anticoagulants and lists the recommended order of blood draw.
The document discusses various components of a basic blood test. It provides details on the normal composition and functions of blood, as well as procedures for blood specimen collection. It also explains the clinical implications and reference ranges for various components analyzed in a complete blood count test, including red blood cells, white blood cells, platelets, hematocrit, hemoglobin, and sedimentation rate.
This document discusses the common types of blood collection test tubes used in hospitals. It explains that red top tubes contain silica clot activators and are used for serum tests like cholesterol and liver enzymes. Purple top tubes contain anticoagulants and are used for blood and fluid cultures. Blue top tubes contain sodium citrate to prevent clotting and are used for coagulation tests. Green top tubes contain heparin and are used for chemistry tests. Lavender top tubes contain EDTA and are used for hematology tests. Gray top tubes contain sodium fluoride and potassium oxalate to prevent glucose breakdown and are used for chemistry tests like glucose. The document stresses that the proper test tube must be chosen according to the diagnostic
The clinical lab provides diagnostic test data to aid in the detection, diagnosis and treatment of disease. The lab is responsible for correct identification, collection and processing of patient specimens, accurate performance of testing, timely reporting of results, and communication with healthcare professionals. There are six main steps in how a sample flows through the lab: 1) test is ordered, 2) sample is collected, 3) sample is delivered to the lab, 4) sample is processed, 5) sample is analyzed, and 6) results are reported. Common specimen types include blood, urine, body fluids, sputum, stool, and tissue samples.
The document discusses different methods of blood collection including capillary, venous, and arterial blood. It describes the equipment and procedures used to collect blood via these methods and the tests that each type is suited for. The document also outlines the various anticoagulants used in blood collection tubes, including EDTA, citrate, heparin, oxalate, and their mechanisms and uses. Finally, it addresses the order that tubes should be drawn in and storage of blood in blood banks.
Blood Transfusion: Procedure and ComplicationsShraddha Madghe
Blood transfusion is a medical procedure where blood or blood components are transferred from one person into the circulatory system of another. There are several types of blood transfusion including allogeneic where the donor and recipient are different people, and autogenic where a person receives their own previously donated blood. The blood transfusion procedure involves verifying the blood type, obtaining consent, administering the transfusion, and monitoring for any complications. Potential complications include transfusion reactions if the blood is mismatched, infections transmitted through the blood, and issues that can arise from using faulty techniques or giving massive amounts of blood.
Urine is commonly used for diagnostic testing and monitoring disease. Proper collection and preservation of urine specimens is important to avoid preanalytical errors. There are various urine collection methods depending on the test and patient, including random, first morning, and timed collections. Preservatives like boric acid and hydrochloric acid are often used to maintain specimen stability, though some tests require no preservative. Careful instruction of patients and labeling of specimens is necessary when collecting 24-hour urine samples.
Automatic And Semi Automatic Analyser Biochemistry MdShamsTabrez4
The AutoAnalyzer is an automated analyzer that uses continuous flow analysis, invented in 1957. It has five main parts: the sample pump, mixing chamber, incubator bath, and detector. AutoAnalyzers were mainly used for routine medical and industrial analyses to increase throughput. While still used for some applications, most instruments are now used industrially and for environmental work.
This document describes different types of blood collection tubes used in phlebotomy and their purposes. It discusses tubes containing various anticoagulants such as EDTA, sodium citrate, and sodium heparin that are used for hematology and coagulation tests. It also describes serum separator tubes containing silica and gel that are used for biochemical and other tests requiring separated serum. The document provides examples of common tests performed using each type of tube.
The document provides information on the various colored blood collection tubes used in laboratories, including their common tests and proper usage. It discusses the purple, pink, blue, yellow, grey, red, dark green, and light green tubes, listing their additives, most common tests, and tips for proper collection techniques. While laboratory preferences may vary, this provides guidance on matching tests to the correct anticoagulated tube.
This document provides information about different colored blood collection tubes and their uses. It discusses the purposes and additives of purple, pink, blue, yellow, grey, and red tubes. The purple, pink, and blue tubes contain anticoagulants and are used for hematology and coagulation tests. The yellow, grey, and red tubes are used for biochemical and other serum-based tests. It provides examples of common tests performed using each tube type and tips for proper sample collection and handling.
The reception in medical labs and blood drawReem Sawalha
This document discusses different types of samples that are collected for clinical testing and the containers and methods used. It outlines the typical order of blood draw and describes various blood collection tubes including EDTA, sodium citrate, plain serum separator, sodium heparin, sodium fluoride tubes and their uses. Other samples that can be collected include urine, stool, cerebrospinal fluid and different types of swabs. Blood culture bottles and blood bags are also summarized. The document concludes with information on arterial sampling, venipuncture and finger stick blood collection methods.
Blood is the most common biologic fluid tested in clinical laboratories. It is usually drawn from a vein into an evacuated collection tube. While blood requires processing to separate plasma or serum from cells, other fluids like urine or cerebrospinal fluid can be tested directly. The type of fluid and analytes being tested determine the appropriate collection and storage methods to ensure test validity.
GENERAL INTRODUCTION TO CHEMICAL PATHOLOGY-SPECIMENS COLLECTION BY DR ABUDU...Xavier875943
This document provides an overview of chemical pathology and clinical chemistry laboratory testing. It discusses common terminology used in chemical pathology, such as reference values and units of measurement. It describes the collection, handling, and preservation of specimens in chemical pathology laboratories. The document outlines the purpose and types of clinical chemistry tests, including tests that measure substances involved in biological functions, waste products, substances indicating cell damage or disease, and drugs or toxins. It discusses the types of specimens used in chemical analysis, including whole blood, serum, plasma, urine, and other fluids. The document provides details on patient preparation, phlebotomy procedures, and factors that can affect test results such as fasting, lipemia, and hemolysis.
Clinical labs are important in diseases diagnosis, determination its severity and patient response to specific treatment. Diagnosis of any disease is first done by physical examination by physician and confirmed by lab diagnostic tests.
Sample collection, Preservation and its EstimationMD Abdul Haleem
This document provides information and guidelines for collecting, preserving, and estimating various types of biological samples for laboratory testing. It discusses the proper collection and handling of blood, urine, tissue, and other samples. Specific details are provided on collecting blood in tubes with different anticoagulants, obtaining urine through timed or random collection, and storing samples at appropriate temperatures. The document also notes important considerations for shipping samples, such as maintaining proper temperatures and documenting collection details.
This document discusses the order of draw for blood collection tubes. It provides information on the different types of blood collection tubes used in phlebotomy, including their purposes, components, and order of collection. The recommended order is: 1) Blood culture bottles, 2) Citrate tubes, 3) Clot activator tubes, 4) Serum separator tubes, 5) Heparin tubes, 6) EDTA tubes, 7) Fluoride tubes. Following the correct order of draw is important to prevent errors and cross-contamination between tubes.
The document summarizes several laboratory tests and procedures used in hematology, physiology, microbiology, and biochemistry. It describes bleeding time and coagulation time tests to assess platelet function. It also outlines the hematocrit test procedure to measure red blood cell volume percentage, and the blood grouping test to determine blood type based on antigen-antibody reactions. Procedures for blood culture collection and centrifugation to separate blood components are provided. Finally, it explains hemoglobin electrophoresis to measure different hemoglobin molecules and identify potential abnormalities.
Clinical laboratories are important for disease diagnosis and monitoring patient health. They examine samples like blood, urine, and CSF to perform tests that help determine disease severity and treatment effectiveness. The main sections of a clinical lab are clinical pathology, hematology, clinical biochemistry, microbiology, serology, and blood bank. Biochemical tests analyze things like lipids, diabetes markers, electrolytes, and bone/liver function. Precise sample collection and handling are crucial to ensure accurate test results.
This document discusses blood collection procedures and anticoagulants. It explains that blood can be collected from veins, capillaries, or arteries for various tests like hematological, biochemical, serological, and molecular examinations. The venipuncture procedure and necessary supplies are outlined. Common anticoagulants like EDTA are described which prevent clotting by chelating calcium. Effects of storage temperature and time on blood cell counts and morphology are also summarized.
This document provides information on the preparation and separation of plasma and serum from whole blood. It explains that plasma can be separated from whole blood by transferring blood to a tube containing anticoagulant and centrifuging, while serum is separated from clotted whole blood. The document discusses proper storage methods for plasma and serum, such as refrigeration or freezing. It also outlines some common errors in plasma/serum preparation like hemolysis and provides tips to prevent hemolysis.
Vacutainer tubes come in different colors that indicate the additive used to preserve samples for specific tests. Purple tubes contain EDTA to prevent clotting for hematology tests. Yellow tubes contain anticoagulants like SPS or ACD to stabilize bacteria for cultures. Red tubes lack additives or contain clot activators for serum biochemistry. Blue tubes contain sodium citrate to evaluate the clotting system. Green tubes contain heparin to inhibit clotting for specialized tests. Grey tubes contain potassium oxalate and sodium fluoride to measure glucose levels. The color-coding helps technicians select the correctly preserved sample for different analyses.
This document provides notes and instructions from an Allied Health instructor to their phlebotomy class. It discusses various venipuncture tests and abbreviations used in lab orders and results. It defines common lab tests and tubes used, including their purposes and appropriate collection methods. The instructor assigns homework and skills practice, and schedules an upcoming exam and review session to further discuss additional lab tests.
All about blood collection and handling, lecture notes to Medical Laboratory Students at Medical Laboratory Technology, Middle Technical University, Baqubah, Iraq
Hematology is the branch of medicine, that is concerned with the study of blood, blood forming organs and blood diseases. It includes study of etiology, diagnosis, treatment, prognosis and prevention of blood diseases .
After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
This document provides guidelines for the proper collection, transportation, preparation, processing, and storage of laboratory samples for disease diagnosis and monitoring. It discusses defining samples and specimens, examples of laboratory samples, requirements for sample collection, potential errors from improper collection, and objectives of collection. Specific guidance is given for collection and handling of various sample types, including blood, urine, cerebrospinal fluid, stool, and others. Proper labeling, transport, separation, preservation, and storage methods are also outlined to ensure sample quality and integrity.
Lecture 1- nitoduction to clinical serology said warsame
This document provides definitions and information about clinical serology. It defines serology as the scientific study of blood serum and antigen-antibody reactions, especially for diagnostic identification of antibodies. The key difference between serum and plasma is that serum is obtained from clotted blood without anticoagulants, while plasma is obtained from anticoagulated blood and contains clotting factors. Serological tests are important for diagnosis of infectious diseases and conditions by detecting antibodies or antigens in serum. The document discusses terms used in serology like sensitivity, specificity, quantitative vs. qualitative tests, and seroconversion. It also provides information on serum and plasma separation methods, disposal of lab waste, and equipment used in serology labs.
This document provides guidance on developing effective presentation skills. It discusses the key steps in preparing and delivering a presentation, including planning the presentation by determining the audience and goals, preparing the content and structure, practicing with visual aids, and presenting confidently with eye contact and engagement. Specific tips are provided for each step, such as using bullet points and simple designs for slides, varying voice pitch for emphasis, and rehearsing thoroughly. Common challenges like lack of practice and confidence are also addressed, along with factors for a successful presentation like being over-prepared and knowing the topic well.
This document contains an index of topics related to biochemistry including diet, vitamins, energy metabolism, lipid metabolism, carbohydrate metabolism, protein metabolism, and molecular biology. The index lists over 75 sub-topics ranging from hem biosynthesis and porphyria to DNA structure, replication, and gene transcription.
Sentence structure II (run-on, comma splice, fragment)HandSome
The document discusses three types of sentence errors: run-on sentences, comma splices, and sentence fragments. Run-on sentences improperly connect two independent clauses without proper punctuation. Comma splices join two independent clauses with only a comma. Sentence fragments are incomplete sentences missing a subject, verb, or both. The document provides examples and explanations of each error and methods to correct them, such as using periods, semicolons, conjunctions, or turning one clause into a dependent clause.
The document discusses the 4 types of sentence structures: simple, compound, complex, and compound-complex. A simple sentence contains one independent clause. A compound sentence joins two independent clauses with a conjunction. A complex sentence contains one independent clause and at least one dependent clause. A compound-complex sentence contains at least two independent clauses and one or more dependent clauses. Examples are provided for each type of sentence structure.
Communication
Communication skills
Communication types
Communication styles
Communication types
Communication essential skills
Communication knowledge
Communication thinking
Communication ways
Communication levels
verbal Communication
nonverbal Communication
Self Disclosure
Parts of speech
Noun
Pronoun
Adverb
Adjective
Interjection
Conjunction
Articles
Uses of Articles
Types of noun
types of pronoun
Ajective types
Adverb of time manner
adverb of place
examples of articles
The document provides examples of how various prepositions are used with time, place, verbs, adjectives, and idiomatic expressions in English. It lists over 50 individual prepositions like "on", "in", "at", "to", "from", "by", etc. and provides context examples to illustrate their usage related to time, place, or with certain verbs, adjectives, or expressions. The prepositions covered indicate time, location, direction, possession, and relationships between objects, people, or ideas in the English language.
.Mascular system
types
muscles
charactersitics
function of muscle
classification of muscles
sarcoplasmic organelles
structure of msucles
how to name muscle
neck muscle
intrinsic back muscle
extrinsic back muscles
anterior compartment of thih muscle
hamstring group muscles
IM injection common sites
muscular tension
software
types of software
operating system
utility software
application software
working of application of different software
examples
famous software
word
excel
powerPoint
Homeostasis refers to the maintenance of a stable internal environment in the body. It involves negative feedback loops that counteract changes to keep properties like temperature and pH levels within normal ranges. The concept of homeostasis was introduced by Walter Cannon in 1930 and forms the basis of physiology. It works through a cyclical system of receptors that detect changes, a control center that activates effectors to correct deviations and restore balance. Most processes use negative feedback loops while some emergency responses employ positive feedback. Disruptions to homeostasis can cause illness.
Hormones are chemical messengers secreted by endocrine glands directly into the bloodstream to regulate bodily functions. The major endocrine glands include the pituitary gland, thyroid gland, adrenal glands, pancreas, testes and ovaries. Hormones function through a process of biosynthesis in endocrine tissues, storage and secretion, transport through blood to target cells, recognition by cell receptors, signal transduction, cellular response, and hormone breakdown. Hormones are classified as steroid hormones, peptide hormones, or amino acid-derived hormones based on their chemical structure and include molecules like insulin, growth hormone, and thyroxine.
The document discusses the auditory ossicles, which are three small bones in the middle ear - the malleus, incus, and stapes. It describes the role of each bone in transmitting sound vibrations from the eardrum to the inner ear. The malleus is attached to the eardrum and transmits vibrations to the incus, which then passes them to the stapes to reach the inner ear. Together, the three bones mechanically transmit sound through a chain reaction of vibrations. The ossicles also help regulate loud sounds by contracting muscles that reduce the eardrum's vibration. Disorders can occur from conditions like otosclerosis or genetic disorders.
the spectrum of anitmicrobial activity
action of antimicrobial drugs
mechanism of antibiotics action
inhibation of protein synthesis
quinolones
Antimeta metabolites
This document discusses the nutritional classification of bacteria into three main categories:
1) Photoheterotrophs, which use light energy but cannot use CO2 as their sole carbon source and obtain energy from organic compounds. Purple non-sulphur bacteria are examples.
2) Chemoheterotrophs, which obtain carbon and energy from organic compounds like glucose. There are saprophytic, parasitic, and symbiotic chemoheterotrophs.
3) Saprophytic bacteria obtain nutrients from decaying organic matter by secreting enzymes. Parasitic bacteria cause diseases in plants and animals. Symbiotic bacteria like nitrogen-fixing rhizobia live in plants and provide fixed nitrogen in exchange
Lecture 6 -- Memory 2015.pptlearning occurs when a stimulus (unconditioned st...AyushGadhvi1
learning occurs when a stimulus (unconditioned stimulus) eliciting a response (unconditioned response) • is paired with another stimulus (conditioned stimulus)
NAVIGATING THE HORIZONS OF TIME LAPSE EMBRYO MONITORING.pdfRahul Sen
Time-lapse embryo monitoring is an advanced imaging technique used in IVF to continuously observe embryo development. It captures high-resolution images at regular intervals, allowing embryologists to select the most viable embryos for transfer based on detailed growth patterns. This technology enhances embryo selection, potentially increasing pregnancy success rates.
10 Benefits an EPCR Software should Bring to EMS Organizations Traumasoft LLC
The benefits of an ePCR solution should extend to the whole EMS organization, not just certain groups of people or certain departments. It should provide more than just a form for entering and a database for storing information. It should also include a workflow of how information is communicated, used and stored across the entire organization.
Histololgy of Female Reproductive System.pptxAyeshaZaid1
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
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How to Control Your Asthma Tips by gokuldas hospital.Gokuldas Hospital
Respiratory issues like asthma are the most sensitive issue that is affecting millions worldwide. It hampers the daily activities leaving the body tired and breathless.
The key to a good grip on asthma is proper knowledge and management strategies. Understanding the patient-specific symptoms and carving out an effective treatment likewise is the best way to keep asthma under control.
The skin is the largest organ and its health plays a vital role among the other sense organs. The skin concerns like acne breakout, psoriasis, or anything similar along the lines, finding a qualified and experienced dermatologist becomes paramount.
Are you looking for a long-lasting solution to your missing tooth?
Dental implants are the most common type of method for replacing the missing tooth. Unlike dentures or bridges, implants are surgically placed in the jawbone. In layman’s terms, a dental implant is similar to the natural root of the tooth. It offers a stable foundation for the artificial tooth giving it the look, feel, and function similar to the natural tooth.
Breast cancer: Post menopausal endocrine therapyDr. Sumit KUMAR
Breast cancer in postmenopausal women with hormone receptor-positive (HR+) status is a common and complex condition that necessitates a multifaceted approach to management. HR+ breast cancer means that the cancer cells grow in response to hormones such as estrogen and progesterone. This subtype is prevalent among postmenopausal women and typically exhibits a more indolent course compared to other forms of breast cancer, which allows for a variety of treatment options.
Diagnosis and Staging
The diagnosis of HR+ breast cancer begins with clinical evaluation, imaging, and biopsy. Imaging modalities such as mammography, ultrasound, and MRI help in assessing the extent of the disease. Histopathological examination and immunohistochemical staining of the biopsy sample confirm the diagnosis and hormone receptor status by identifying the presence of estrogen receptors (ER) and progesterone receptors (PR) on the tumor cells.
Staging involves determining the size of the tumor (T), the involvement of regional lymph nodes (N), and the presence of distant metastasis (M). The American Joint Committee on Cancer (AJCC) staging system is commonly used. Accurate staging is critical as it guides treatment decisions.
Treatment Options
Endocrine Therapy
Endocrine therapy is the cornerstone of treatment for HR+ breast cancer in postmenopausal women. The primary goal is to reduce the levels of estrogen or block its effects on cancer cells. Commonly used agents include:
Selective Estrogen Receptor Modulators (SERMs): Tamoxifen is a SERM that binds to estrogen receptors, blocking estrogen from stimulating breast cancer cells. It is effective but may have side effects such as increased risk of endometrial cancer and thromboembolic events.
Aromatase Inhibitors (AIs): These drugs, including anastrozole, letrozole, and exemestane, lower estrogen levels by inhibiting the aromatase enzyme, which converts androgens to estrogen in peripheral tissues. AIs are generally preferred in postmenopausal women due to their efficacy and safety profile compared to tamoxifen.
Selective Estrogen Receptor Downregulators (SERDs): Fulvestrant is a SERD that degrades estrogen receptors and is used in cases where resistance to other endocrine therapies develops.
Combination Therapies
Combining endocrine therapy with other treatments enhances efficacy. Examples include:
Endocrine Therapy with CDK4/6 Inhibitors: Palbociclib, ribociclib, and abemaciclib are CDK4/6 inhibitors that, when combined with endocrine therapy, significantly improve progression-free survival in advanced HR+ breast cancer.
Endocrine Therapy with mTOR Inhibitors: Everolimus, an mTOR inhibitor, can be added to endocrine therapy for patients who have developed resistance to aromatase inhibitors.
Chemotherapy
Chemotherapy is generally reserved for patients with high-risk features, such as large tumor size, high-grade histology, or extensive lymph node involvement. Regimens often include anthracyclines and taxanes.
Know the difference between Endodontics and Orthodontics.Gokuldas Hospital
Your smile is beautiful.
Let’s be honest. Maintaining that beautiful smile is not an easy task. It is more than brushing and flossing. Sometimes, you might encounter dental issues that need special dental care. These issues can range anywhere from misalignment of the jaw to pain in the root of teeth.
Summer is a time for fun in the sun, but the heat and humidity can also wreak havoc on your skin. From itchy rashes to unwanted pigmentation, several skin conditions become more prevalent during these warmer months.
Kosmoderma Academy, a leading institution in the field of dermatology and aesthetics, offers comprehensive courses in cosmetology and trichology. Our specialized courses on PRP (Hair), DR+Growth Factor, GFC, and Qr678 are designed to equip practitioners with advanced skills and knowledge to excel in hair restoration and growth treatments.
Demystifying Fallopian Tube Blockage- Grading the Differences and Implication...
Blood Collection Tube,ubi.
1. Ubi Ahmad
Blood Collection Tube:
Vacutainer blood collection tube is a sterile and plastic test tube with a colored rubber top stopper
creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid.
For the example blood sample
Phlebotomy
Collection of the patent blood specimen to send the laboratory for testing, diagnostic and research.
Phlebotomy is a procedure in which a needle is used to take blood specimen from a vein, usually for
laboratory testing.
1. Blood Culture Bottle
Purple:
• Generally used for hematology tests where whole blood is required for analysis.
• These tubes contain EDTA (ethylenediaminetetraacetic acid) which act as a potent anticoagulant
by binding calcium to blood.
Common tests:
o Full blood count FBC
o Erythrocyte sedimentation rate ESR
o Blood film for abnormal cells or malarial parasites
o Reticulocytes / hbA1C for DM patient
o Red blood folate / parathyroid hormone PTH
Pink:
Same as the purple one but are specifically used only for white blood samples.
Common tests:
o Group and save (G&S)
o Cross match (CM)
o Direct coomb’s test aka direct antiglobulin test for autoimmune haemolytic anemia.
Blue:
Blue is used for haemotolgy tests involving the clotting system, which require inactivated whole blood
for analysis. This contains buffered sodium citrate, which acts as a reversible anticoagulant by binding
the calcium ion in the blood and subsequently disrupting the clotting cascade.
Common tests:
2. Ubi Ahmad
o Coagulation screening includes bleeding time for platelet function / prothrombin time PT for
extrinsic pathway, / activated partial thromboplastin APTT for intrinsic pathway and Thrombin
time TT or fibrinogen assay for final common pathway.
o D-timer thrombosis, e.g., due to DHV or PE
Yellow:(aka gold)
Bottle used for huge variety of tests requiring separate serum for analysis including biochemistry,
endocrinology, oncology, toxicology, microbiology and immunology.
This tube is known in lab as SST (serum separating tube). It contains 2 agents, silica particles &
serum separating gel. This silica particles works to activate clotting and cause the blood to cells to
clump together.
Common tests:
o Urea & electrolytes (Includes, UREA/CRETANIN/SODIUM/POTTASIUM)
o C-reactive protein (CRP)
o LFT’s this includes BILRUBIN/ALP/AST/ALT/GGT/TOTAL PROTEIN/ALBUMIN)
o Amylase assay
o Bone profile includes CALCIUM/POTASSIUM/ALP/ALBUMIN.
o Magnesium assay / thyroid function test / troponins/urate/creatinine kinase/serum
osmolality
Red:
• Contain silica particles which act as a clot activator.
• Used for biochemistry tests requiring serum
• Used for many sensitive tests including hormones, toxicology, drug levels, bacterial, viral
serology, antibodies.
Dark green:
• Contains sodium heparin which acts as anticoagulant.
Common tests:
o Ammonia
o Insulin
o Renin & aldosterone.
White:
• Used for molecular diagnostics such as PCR and DNA amplification studies.