The clinical lab provides diagnostic test data to aid in the detection, diagnosis and treatment of disease. The lab is responsible for correct identification, collection and processing of patient specimens, accurate performance of testing, timely reporting of results, and communication with healthcare professionals. There are six main steps in how a sample flows through the lab: 1) test is ordered, 2) sample is collected, 3) sample is delivered to the lab, 4) sample is processed, 5) sample is analyzed, and 6) results are reported. Common specimen types include blood, urine, body fluids, sputum, stool, and tissue samples.
Challenges in interpreting serum protein electrophoresis. Requires an approach to recognize pattern within the various protein fractions & differentiate systemic inflammatory response from abnormal antibody production due to neoplastic disorders.Presence of M-band does not always correlate with plasma cell disorders but can be seen some lymphomas, chronic leukaemias, systemic amyloidosis hence need further ancillary tests for diagnosis of aetiology for the M-band.
Biochemistry is the study of the structure and function of biological molecules such as proteins, nucleic acids, carbohydrates and lipids.
Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions.
Biochemistry deals with body substance like enzymes, carbohydrates, amino acids, fats, proteins, hormones, DNA, RNA, pigments etc.
The major objective of biochemistry is the complete understanding of all chemical processes associated with living cells at the molecular level. Some of the objectives can be listed as follows:
1. Isolation, structural elucidation and the determination of mode of action of biomolecules.
2. Identification of disease mechanisms.
3. Study of in born errors of metabolism.
4. Study of oncogenes in cancer cells.
5. The relationship of biochemistry with the genetics, physiology, immunology, pharmacology, toxicology etc.
Biochemistry is related to almost all the life sciences and without biochemistry background and knowledge, a through understanding of health and well-being is not possible.
Challenges in interpreting serum protein electrophoresis. Requires an approach to recognize pattern within the various protein fractions & differentiate systemic inflammatory response from abnormal antibody production due to neoplastic disorders.Presence of M-band does not always correlate with plasma cell disorders but can be seen some lymphomas, chronic leukaemias, systemic amyloidosis hence need further ancillary tests for diagnosis of aetiology for the M-band.
Biochemistry is the study of the structure and function of biological molecules such as proteins, nucleic acids, carbohydrates and lipids.
Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions.
Biochemistry deals with body substance like enzymes, carbohydrates, amino acids, fats, proteins, hormones, DNA, RNA, pigments etc.
The major objective of biochemistry is the complete understanding of all chemical processes associated with living cells at the molecular level. Some of the objectives can be listed as follows:
1. Isolation, structural elucidation and the determination of mode of action of biomolecules.
2. Identification of disease mechanisms.
3. Study of in born errors of metabolism.
4. Study of oncogenes in cancer cells.
5. The relationship of biochemistry with the genetics, physiology, immunology, pharmacology, toxicology etc.
Biochemistry is related to almost all the life sciences and without biochemistry background and knowledge, a through understanding of health and well-being is not possible.
In the continuous quality journey, Controlling laboratory Errors is an integral part & focusing on analytical, post-analytical process is the first step. Developing a reporting culture followed by thorough analysis and implementation of appropriate corrective, preventive actions is required.
Biochemical kidney function tests with their clinical applicationsrohini sane
An illustrative presentation on Biochemical kidney function tests with their clinical applications for medical ,dental, pharmacology and biotechnology student to facilitate easy-learning.
In the continuous quality journey, Controlling laboratory Errors is an integral part & focusing on analytical, post-analytical process is the first step. Developing a reporting culture followed by thorough analysis and implementation of appropriate corrective, preventive actions is required.
Biochemical kidney function tests with their clinical applicationsrohini sane
An illustrative presentation on Biochemical kidney function tests with their clinical applications for medical ,dental, pharmacology and biotechnology student to facilitate easy-learning.
Hematology is the branch of medicine, that is concerned with the study of blood, blood forming organs and blood diseases. It includes study of etiology, diagnosis, treatment, prognosis and prevention of blood diseases .
After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
State of ICS and IoT Cyber Threat Landscape Report 2024 preview
Clinical laboratory basic
1. Clinic Lab : Basic Overview
Training Design: Dorra Hung
2. Laboratory Roles & Responsibilities
The clinical lab provides diagnostic test data to aid in the detection,
diagnosis and treatment of disease. Data is used by physicians,
nurses, pharmacists and other healthcare professionals.
The responsibilities of the clinical lab include:
Correct identification, collection and processing of patient specimens
Accurate performance of testing
Timely reporting of results
Communication with physicians and other healthcare professionals
Analyst testing is used to help diagnose, monitor or treat disease
Page 2 Mar-07 Dorra Hung
3. Laboratory Workflow
There are six main steps in how a
sample flows through the lab
from order creation to final test
result.
1. Test is ordered.
2. Sample is collected
3. Sample is delivered to the lab.
4. Sample is processed.
5. Sample is analyzed.
6. Results are reported.
Page 3 Mar-07 Dorra Hung
4. Laboratory Specimens
Most common Laboratory Specimen Types:
Blood
Urine
Additional Laboratory Specimens:
Body fluids
Sputum
Stool
Tissue samples
Culture swabs
Page 4 Mar-07 Dorra Hung
8. Medical Examination
Objective is to get an answer about the health status of a patient
The physician determines on the basis of the anamneses, his clinical
examination and on the basis of additional known information an
Enquiry Examination Request
This is followed by the necessary preparation of the patient and blood
sampling
Page 8 Mar-07 Dorra Hung
9. Pre-Analytical
Common material for examination
Venous Blood (Serum or Plasma)
Capillary blood
Urine (Single shot or 24 hour collection)
Cerebrospinal fluid (CSF)
Puncture Fluids
Others, such as Faeces, Saliva, Gastric acid, Hair…..
Page 9 Mar-07 Dorra Hung
10. What is Blood?
Blood Composition 55% Plasma
Plasma Yellow, sticky liquid
Cells Transport of
Nutrients (proteins, fats,
carbon hydrates)
Hormones
44% Erythrocytes
Red blood cells
Contain Haemoglobin
O2 and CO2 transport
Page 10 Mar-07 Dorra Hung
11. What is Blood?
Blood Composition 0.1% Leucocytes
Plasma White blood cells
Cells Protection against
bacteria
viruses
0.9% Thrombocytes
Platelets
Coagulation at injuries
Page 11 Mar-07 Dorra Hung
12. Sample containers - What do we use?
Tube size (mm) Draw Volume
ID x Height (mL)
13 x 75 3.5
Red top Vacutainer® collection 13 x 100 4.0, 5.0,
tubes are used for 16 x 100 5.0, 6.0, 7.0, 8.0
serum determinations 16 x 100 8.5
in chemistry. Stopper Coagulant Use
They contain NO coagulant. color
Lavender EDTA Hematology
The grey and red speckled SST™ Light Blue Sodium citrate Coagulation
tube at left (“tiger top”) contains a Green Lithium Plasma
polymer gel for serum separation heparate chemistry
and has a Hemoguard™ tube Light Green Lithium Serum
heparate + gel chemistry
closure. Grey Sodium citrate Glucose
testing
The Vacutainer® at right has a
conventional tube stopper,
Tubes are now made of plastic to
help protect personnel from injury
and bloodborne pathogens.
Vacutainer® and Hemogard™ are trademarks of Becton, Dickinson & Company.
Page 12 Mar-07 Dorra Hung
13. What samples do we analyze?
Phlebotomist draws
sample
Page 13 Mar-07 Dorra Hung
14. Plasma versus Serum
Blood to which an anticoagulant has been Blood to which no anticoagulant has
added will not clot. Blood cells will settle to the been added will clot. Blood cells get
bottom of the tube leaving plasma at the top of caught in the clot leaving serum
the tube. behind.
Page 14 Mar-07 Dorra Hung
15. Pre-Analytical
Possible Influences
Age, gender
Genetic influences
Nutritional influences
Pregnancy
Biorhythm (diurnal rhythm causing analytical fluctuations)
Muscular mass, body weight
Physical activity or inactivity
Psychological stress (fear for blood collection, surgery)
Use of medicines
Page 15 Mar-07 Dorra Hung
16. Pre-Analytical
Disturbing Influences
Sample collection (body position, venous congestion, ….)
Sample condition (haemolytic, lipemic, icteric)
Normal serum obtained from an individual in good health is usually
clear, pale yellow in color. However, the color of the patient’s serum
may appear different for various reasons such as disease or improper
handling of the blood specimen.
Lipemia (Lipe) results from increased levels of lipoproteins associated
with triglycerides, and it can cause the serum to appear white.
Hemolysis (Heme) is caused usually by the release of hemoglobin
from ruptured red blood cells during sample collection and/or sample
handling. This interference can cause the serum to appear red.
Page 16 Mar-07 Dorra Hung
17. Pre-Analytical
Icterus (Icte) is the result of increased levels of bilirubin, and it can cause
the serum to appear yellow.
Page 17 Mar-07 Dorra Hung
19. Pre-Analytical
After the centrifugation if the sample was without anticoagulant the supernatant
fluid is SERUM otherwise is plasma .
As anticoagulants they use EDTA K3 , EDTA K2 , Heparin, Citric acid 9:1, Citric
Acid 4:1 NaF and others.
If we use plasma we must know the type of the anti-coagulant due to different
interferences f.e.
Ca , Na , Fe , ALP ...
21. Pre-Analytical
Some photometric assays may be influenced by the presence of these
abnormal serum colors and the reliability of the test results may be
decreased.
haemolysis can cause analytical interferences such as high K+
caused by release from erythrocytes, or can interfere with the
measuring technique (photometry)
Inadequate sample transport
Wrong centrifugation
Inadequate sample storage (Bilirubin)
Page 21 Mar-07 Dorra Hung
22. Pre-Analytical
Different type of sample collection in commercially available blood collection
systems (Beckton Dickinson Vacutainer, Sarstedt Monovetten, …..)
Tube with additional Extraction of Application Colour-
Anti-Coagulation agent coding
Whole blood (without Serum Clinical Chemistry, Serology, Red
agent) Immunochemistry
Heparin Plasma Clinical Chemistry Green
EDTA Plasma Haematology, Special Lilac
Chemistry, Immunochemistry
Citrate Plasma Coagulation tests Bleu
Na-Fluoride / K-Oxalate Plasma Glucose, Lactate Grey
Page 22 Mar-07 Dorra Hung
23. Pre-Analytical
Serum
Plasma versus Serum 30-45 minutes clothing (preferably
in the dark)
10-15 minutes centrifugation
@ 1000-1500 g
Plasma
Blood to which an anticoagulant has been
added will not clot. Blood cells will settle to the
Blood to which no anticoagulant has
been added will clot. Blood cells get
Immediate 10-15 minutes
bottom of the tube leaving plasma at the top of caught in the clot leaving serum
the tube. behind.
centrifugation @ 1000-1500 g
For internal use only
Page 7 Jan-07 Velemirov / Twisk EU Sales Training
Page 23 Mar-07 Dorra Hung
24. Pre-Analytical
Sample transport and storage
Properly packed
Transport must be save
Bio hazardous material
4 hours stable @ 15-25 oC
Closed to avoid evaporation
24 hours stable @ 4-8 oC
Dry ice, cool packs, refrigerator, etc.
Page 24 Mar-07 Dorra Hung
25. Pre-Analytical
Example: Potasium
Plasma is recommended for rapid centrifugation
Use only serum or plasma from single patients
Sample preparation (heparin plasma)
Centrifuge within 30-45 minutes after collection
Erythrocytes produce Homocysteine, which continues after
sampling
Store on ice if centrifugation within 30-45 minutes is not possible
Store plasma at -20 oC if sample can not be measured within 48
hours
Page 25 Mar-07 Dorra Hung
27. Analytical
Adequate test methodology
Standard Operating
Procedure
Understandable
Traceable
Routine test must be
Easy to be executed
Reliable
Low risk on failure
Page 27 Mar-07 Dorra Hung
28. Statistical Quality Control
Samples with known concentration
Low
Medium
High
As part of the daily routine
Begin of the run
Middle in the run
End of the day
Random
Page 28 Mar-07 Dorra Hung
31. Patient Result
Test Report
Demographic information
Patient name, Patient ID, Lab number
Sample matrix, Visual distortions
Date, Time
Sample collection, Arrival in the lab, Time of analyses
Analytical results
Test name, Unit, Reference values, Comments (high/low, diluted,
duplicates, ……)
Page 31 Mar-07 Dorra Hung
32. Analytical Results
Expected Values
Reference Range
Normal values
Based on a large pool
of healthy persons
Differences between
Children vs. adults
Male vs. female
Serum vs. plasma
Population
Biorhythm
Page 32 Mar-07 Dorra Hung
33. Diagnose
After checking the reliability of the analysis
Analytical range
Statistical Quality control
Pre-analytical and analytical disturbances
Plausibility of the result
Compared with previous results
Fit with the situation of the patient
DIAGNOSE
Page 33 Mar-07 Dorra Hung
34. Methods of Clinical Chemistry
Photometry
Chemiluminence
Potentiometry (ISE)
Electrophoresis
Nephelometry
γ- COUNTER
Mass absorption
Osmometry
HPLC
TLC
Coagulation
...
35. Optical Density A = - log 10 T
Photometry A= ε x L x C
Page 35 Mar-07 Dorra Hung
47. Direct potentiometry : This is the simplest method of making ion-
selective electrode measurements. The electrodes are immersed in
a test solution and the electrode potential is measured directly with
a millivolt meter. The concentration is then related directly to this
measurement by reading the answer from a calibration graph of
concentration versus millivolts.
Indirect potentiometry : Dilution of the sample (less volume, less
problems, less interventions
Page 47 Mar-07 Dorra Hung
49. Pre-analytical factors that affect serum proteins
concentrations
1)Time of the day
2)Position
3)Exercise
4)Fasting vs non fasting
5)Medications
6)Time of year (season)
7)Age and gender
8)Geographic location
9)Venipuncture technique
10)Sample handling and storage
Page 49 Mar-07 Dorra Hung
50. Patient Result
Test Report
Demographic information
Patient name, Patient ID, Lab number
Sample matrix, Visual distortions
Date, Time
Sample collection, Arrival in the lab, Time of analyses
Analytical results
Test name, Unit, Reference values, Comments (high/low, diluted,
duplicates, ……)
Page 50 Mar-07 Dorra Hung
51. Auto-validation
Automatic
Limits defined by the lab
Delta checks
Quality control with Westgard rules
Messages from the systems
Page 51 Mar-07 Dorra Hung