1. The document discusses the metabolism of purine and pyrimidine nucleotides, including their biosynthesis, degradation, and disorders related to purine metabolism such as gout and Lesch-Nyhan syndrome.
2. Purine nucleotides are synthesized through both a de novo synthesis pathway that builds the purine ring from simple precursors, and a salvage pathway that recycles purine bases. A key early intermediate is IMP, which is converted to AMP and GMP.
3. Uric acid is the final product of purine degradation in humans and high levels can cause gout. Lesch-Nyhan syndrome results from HGPRT deficiency disrupting the salvage pathway.
explains the breakdown of purine. source and excretion of purine is explained. hyperuricemia and hypouricemia is discussed. types of Gout, clinical features and treatment is included.
Biosynthesis of pyrimidine nucleotides can occur by a de novo pathway or by the reutilization of preformed pyrimidine bases or ribonucleosides (salvage pathway).
The pyrimidine synthesis is a similar process than that of purines. In the de novo synthesis of pyrimidines, the ring is synthesized first and then it is attached to a ribose-phosphate to for a pyrimidine nucleotide.
explains the breakdown of purine. source and excretion of purine is explained. hyperuricemia and hypouricemia is discussed. types of Gout, clinical features and treatment is included.
Biosynthesis of pyrimidine nucleotides can occur by a de novo pathway or by the reutilization of preformed pyrimidine bases or ribonucleosides (salvage pathway).
The pyrimidine synthesis is a similar process than that of purines. In the de novo synthesis of pyrimidines, the ring is synthesized first and then it is attached to a ribose-phosphate to for a pyrimidine nucleotide.
BIOSYNTHESIS OF PYRIMIDINES
SALVAGE PATHWAY
DE NOVO PATHWAY
SYNTHESIS OF OTHER PYRIMIDINE NUCLEOTIDES
IMPORTANCE
Essential building blocks of nucleic acids
Biologically very important heterocycles
Used in anti-biotics, used as anti-bacterial and anti-fungal also
Derivatives of pyrimidine also possess good anti-viral properties
BIOSYNTHESIS OF PYRIMIDINES
SALVAGE PATHWAY
DE NOVO PATHWAY
SYNTHESIS OF OTHER PYRIMIDINE NUCLEOTIDES
IMPORTANCE
Essential building blocks of nucleic acids
Biologically very important heterocycles
Used in anti-biotics, used as anti-bacterial and anti-fungal also
Derivatives of pyrimidine also possess good anti-viral properties
Nucleic acid
Nucleic acids are the polymer of nucleotides (polynucleotides) held by 3’ and 5’ phosphate bridge.
Nucleotide
Nucleotide perform variety of functions in a living cells. They are composed of pentose sugar, phosphate and a nitrogenous base.
Functions of Nucleotide
Monomeric units of DNA and RNA
Structural component of several coenzymes e.g. FAD, NADP+
Serve as carriers of high energy intermediates in the biosynthesis of carbohydrates, lipids and proteins
Nucleotides are intimately involved in the energy reactions of the cell e.g. ATP
Control several metabolic reactions by their action as allosteric regulators.
Cyclic AMP amd cyclic GMP are the second messenger in hormonal functions.
Nucleotide structure
Biosynthesis of Purines
Biosynthesis of Pyrimidine
Disorder of Purine
Catabolism of Purine
introduction of Purine and Pyrimidine metabolism, biosynthesis and degradation of nucleotides, biological functions and metabolic disorders, chemical analogues and therapeutic drugs, uric acid metabolism
De novo and salvage pathway of nucleotides synthesis.pptx✨M.A kawish Ⓜ️
This slides explains Metabolism topic "De novo and salvage pathway of nucleotides synthesis. In which synthesis of Purines and pyrimidines synthesis has been occurred. In last there is a difference between these two pathways.
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Purine nuecleotide
1. METABOLISM OF PURINE AND
PYRIMIDINE NUCLEOTIDES
Dr Bakhtawar farooq
Nishter Medical university
Biochemistry,
2. Biosynthesis of purine nucleotides:
• The three processes that contribute to
purine nucleotide biosynthesis are.
1. Synthesis from amphibolic intermediates
( synthesis de novo ).
2. Phosphoribosylation of purines.
3. Phosphorylation of purine nucleosides.
3. Purines are synthesized by most of the tissues ,the
major site is liver.Subcellular site -- cytoplasm
• Denovo synthesis:Major pathway
Synthesis of purine nucleotides from
various small molecules derived as
intermediates of many metabolic pathways
in the body.
• Salvage pathway: Minor pathway
5. • Parent purine nucliotide first synthesised is
INOSINE MONO PHOSPHATE (IMP)
It is a nucleotide composed of (HYPOXANTHINE +
RIBOSE + PHOSPHATE )
• From IMP other purine nucleotides are
synthesized, like
• AMP(adenosine mono phosphate)
• GMP(guanosine mono phosphate)
11. Biosynthesis of Purine Ribonucleotides
1. Ribose 5-phosphate, produced in the hexose
monophosphate shunt of carbohydrate metabolism is
the starting material for purine nucleotide synthesis.
It reacts with ATP to form
phsophoribosyl pyrophosphate (PRPP).
PRPP Synthetase is inhibited by PRPP
12. 2. Glutamine transfers it’s amide nitrogen to PRPP
to replace pyrophosphate and produce 5-
phosphoribosylamine.
The enzyme PRPP glutamyl amidotransferase is
controlled by feedback inhibition of nucleoltides (IMP,
AMP and GMP,).
13. 3. Phosphoribosylamine reacts with glycine in the
presence of ATP to form glycinamide ribosyl 5-
phosphate or glycinamide ribotide (GAR).
14. 4. N5,N10 formyl tetrahydrofolate donates the formyl
group and the product formed is formylglycinamide
ribosyl 5- phosphate.
15. 5. Glutamine transfers the second amido amino
group to produce formylglycinamideine ribosyl 5-
phosphate.
Gln Glu
ATPmg+
Synthetas
e
•
16. 6. The imidazole ring of the purine is closed in an ATP
dependent reaction to yield 5-aminoimidazole
ribosyl 5-phosphate
H2O
ATP mg+
SYNTHETASE
17. 7. Incorporation of CO2 (carboxylation) occurs to yield
aminoimidazole carboxylate ribosyl 5-phosphate.
This reaction does not require the vitamin biotin and /or
ATP which is the case with most of the carboxylation
reaction.
CO2
8. Carboxylase
18. 8. Aspartate condenses with aminoimidazole
carboxylate ribosyl 5-phosphate. to form
aminoimidazole 4-succinyl carboxamide ribosyl 5-
phosphate.
aspertate H2O
synthetase
19. 9. Adenosuccinase cleaves off fumarte and only the amino
group of aspartate is retained to yield aminoimidazole 4-
carboxamide ribosyl 5-phosphate.
9. f
fumarate
arginosuccinase
20. 10. N10 formyl tetrahydrofolate donates a one-carbon
moiety to produce formimidoimidazole 4-carboxamide
ribosyl 5-phosphate.
With this reaction, all the carbon and nitrogen atoms of
purine ring are contributed by the respective sources.
21. 11. The final reaction catalysed by cyclohydrolase leads to
ring closure with an elimination of water molecule
from formimidoimidazole ribosyl-5-P by Inosine -
monophosphate (IMP) cyclohydrolase forms IMP.
22. Synthesis of AMP and GMP from IMP
• Inosine monophosphate is the immediate precursor for
the formation of AMP & GMP
• Aspertate condences with IMP in the presence of GTP to
produce sdenylosuccinate which on cleavage forms AMP.
• For the synthesis of GMP, IMP undergoes
NAD+ dependent dehydrogenation to form Xanthosine
monophosphate ( XMP). Glutamine then transfers amide
nitrogen to XMP to produce GMP.
23.
24. Inhibitors of purine synthesis.
1. Sulfonamides are the structural analogs of
paraaminobenzoic acid (PABA).
these sulfa drugs can be used to inhibit the synthesis of
folic acid by microgranisms.
this indirectly reduces the synthesis of purines and
therefore, the nucleic acids (DNA and RNA).
sulfonamides have no influence on humans, since folic
acidf is not synthesized and is supplied through diet.
25. The structural analogs of folic acid (eg: methotrexate) are
widely used to control cancer.
They inhibit the synthesis of purine nucleotides and thus
nucleic acids.
These inhibitors also affect the proliferation of normally
growing cells.
This causes many side-effects including
anemia, baldness, scaly skin etc.
26. SALVAGE PATHWAY FOR PURINES
• The free purines ( adenine, guanine & hypoxanthine ) are
formed in the normal turnover of nucleic acids & also
obtained from the dietary sources.
• The purines can also be converted to corresponding
nucleotides, & this process is known as ‘salvage
pathway’.
27. • Adenine phosphoribosyl transferase catalyses the
formation of AMP from adenine.
• Hypoxanthine-guanine phosphoribosyl transferase
(HGPRT) converts guanine & hypoxanthine respectively, to
GMP & IMP.
• Phosphoribosyl pyrophosphate (PRPP) is the donor of
ribose 5 phosphate in the salvage pathway.
• The salvage pathway is perticularly important in certain
tissues such as erythrocytes & brain where denovo
synthesis of purine nucleotides is not operative.
28.
29.
30.
31. REGULATION OF PURINE NUCLEOTIDE
BIOSYNTHESIS
• The purine nucleotide synthesis is well coordinated to
meet the cellular demands.
• The intracellular concentration of PRPP regulates purine
synthesis to a large extent.
• This inturn is dependent on the availability of ribose 5
phosphate & the enzyme PRPP synthetase.
32. • PRPP glutamyl amidotransferse is controlled by a
feedback mechanism by purine nucleotides.
• If AMP & GMP are available in adequate amounts to meet
the cellular requirements, their synthesis is turned off at
the amidotransferase reaction.
• Another important stage of regulation is in the convertion
of IMP to AMP & GMP.
• AMP inhibits adenylosuccinate synthetase while GMP
inhibits IMP dehydrogenase.
• Thus, AMP & GMP control their respective synthesis from
IMP by a feedback mechanism.
35. CONVERTION OF RIBONUCLEOTIDES TO
DEOXY RIBONUCLEOTIDES
• The synthesis of purine & pyrimidine deoxy
ribonucleotides occur from ribonucleotides by a reduction
at the C2 of ribose moity.
• This reaction is catabolised by enzyme ribonucleotide
reductase.
• The enzyme ribonucleotide reductase itself provides the
hydrogen atoms needed for reduction from its sulfhydryl
groups.
36. • The reducing equivalents, in turn, are supplied by
Thioredoxin, a monomeric protein with two cysteine
residues.
• NADPH-dependent thioredoxin reductase converts the
oxidised thioredoxin to reduced form.
37.
38. DEGREDATION OF PURINE NUCLEOTIDES
• The end product of purine metabolism in
humans is uric acid.
1. The nucleotide monophosphates (AMP, IMP & GMP ) are
converted to their respective nucleoside forms
(adenosine,inosine & guanosine ) by the action of
nucleosidase.
2. The amino group, either from AMP or adenosine, can be
removed to produce IMP or inosine respectively.
39.
40. 3. Inosine & guanosine are, raspectively, converted to
hypoxanthine & guanine (purine bases) by purine
nucleoside phosphorylase.
4. Adenosine is not degreded by this enzyme, hence it has
to be converted to inosine.
5. Guanine undergoes deamination by guanase to form
xanthine.
41. • Xanthine oxidase is an important enzyme that converts
hypoxanthine to xanthine, & xanthine to uric acid.
• This enzyme contains FAD, Molybdenum & Iron, & is
exclusively found in liver & small intestine.
• Xanthine oxidase liberates H2O2 & H2O which is harmful
to the tissues.
• Catlase cleaves H2O2 to H2O & O2.
42. • Uric acid ( 2,6,8-ttioxopurine ) is the final excretory
product of purine metabolism in humans.
• Uric acid can serve as an important antioxidant by getting
itself converted non enzymatically to allantoin.
• It is believed that the antioxidant role of ascorbic acid in
primates is replaced by uric acid, since these animals have
lost the ability to synthesize ascorbic acid.
43. • Most animals ( other than primates ) however oxidise uric
acid by the enzyme uricase to allantoin, where the purine
ring is cleaved.
• Allantoin is then converted to allantoic acid & excreated in
some fishes.
• Further degradation of allantoic acid may occur to
produce urea ( in amphibians, most fishes & some
molluscs ) &, later, to ammonia (in marine invertebrates).
44. DISORDERS OF PURINE METABOLISM
1. HYPERURICEMIA AND GOUT
Uric acid is the end product of purine metabolism in
humans.
The normal concentration of uric acid in the serum of
adults is in the range of 3-7 mg / dl.
In women, it is slightly lower ( by about 1 mg ) than in
men.
The daily excreation of uric acid is about 500-700 mg.
45. • Hyperuricemia refers to an elevation in the serum uric
acid concentration.
• This is sometimes associated with increased uric acid
excreation ( Uricosuria)
• GOUT is metabolic disease associated with
overproduction of uric acid.
• At the physiological pH, uric acid is found in a more
soluble form as sodium urate.
• In severe hyperuricemia, crystals of sodium urate get
deposited in the soft tissues, perticularly in the joints.
46. • Such deposits are commonly known as tophi.
• This causes inflammation in the joints resulting in a
painful gouty arthritis. Typical gouty arthritis affects first
metatarsophalangeal joint.(GREAT TOE).
• Sodium urate &/or uric acid may also precipitate in
kidneys & ureters that result in renal damage & stone
formation.
• Historically, gout was found to be often associated with
high living, over-eating & alcoho consumption.
• The prevalence of gout is about 3 / 1,000 persons, mostly
affecting males.
47. Clinical features:
•Attacks are precipitated by alcohol intake.
Often patient have few drinks , go to sleep
symptomless , but are awakened during
early hours by severe joint pains.
Synovial fluid shows birefringent crystals under
polar microscope is diagnostic.
48. • GOUT IS OF TWO TYPES
1. PRIMARY GOUT.
It is an inborn error of metabolism due to
overproduction of uric acid.
This is mostly related to over production of purine
nucleotides.
• PRPP synthetase : in normal circumstances , PRPP
synthetase is under feedback control by purine
nucleotides ( ADP & GDP ).
However, varient forms of PRPP synthetase-which are
not subjected to feedback regulation-have been
detected. This leades to increased production of purines.
49. • PRPP glutamylamidotransferse :
The lack of feedback control of this enzyme by purine
nucleotides also leads to their elevated synthesis.
• HGPRT deficiency : This is an enzyme of purine salvage
pathway, & its defect causes Lesch-Nyhan syndrome. This
disorder is associated with increased synthesis of purine
nucleotides by a two fold mechanism.
Firstly, decreased utilization of purines ( Hypoxanthine &
guanine ) by salvage pathway, resulting in the
accumulation & divertion of PRPP for purine nucleotides.
Secondly, the defect in salvage pathway leads to
decreased levels of IMP & GMP causing impairment
in the tightly controlled feedback regulation of their
production.
50. Glucose 6-phosphatase dificiency:
Intype I glycogen storage disease ( von-gierke’s ), glucose-6-
phosphate cannot be converted to glucose due to the
deficiency of glucose-6-phosphatase.
This leads to the increased utilazation of glucose-6-phosphate
by HMP shunt resulting in elevated levels of ribose-5-
phosphate & PRPP &, ultimately, purine overproduction.
von gierke’s disease is also associated with increased activity
of glycolysis.
Due to this, lactic acid accumulates in the body which
interferes with the uric acid excretion through renal tubules.
51. ELEVATION OF GLUTATHIONE REDUCTASE :
varient of glutathione reductase generates more
NADP+ which is utilized by HMP shunt .
This leads to increased ribose 5-phosphate and
PRPP synthesis.
52. Secondary gout:
Secondary hyperuricemia is due to various diseases causing
increased synthesis or decreased excretion of uric acid.
Increased degradation of nucleic acids (hence more Uric acid
formation) is observed in various cancers (leukemias,
polycythemias, lymphomas, etc).
Psoriasis and increased tissue breakdown (trauma,
starvation etc).
53. Treatment of Gout:
The drug of choice for the treatment of primary gout is
allopurinol.
This is a structural analog of hypoxanthine that
competitively inhibits the enzyme xanthine oxidase.
Further allopurinol is oxidized to alloxanthine by xanthine
oxidase.
Alloxanthine, in turn is a more effective inhibitor of xanthine
oxidase. This type of inhibition is referred to as suicide
inhibition.
54. Inhibition of xanthine oxidase by allopurinol leads to the
accumulation of hypoxanthine and xanthine.
These two compounds are more soluble than uric acid,
hence easily excreted.
Besides the drug therapy, restriction in dietary intake of
purines and alcohol is advised.
Consumption of plenty of water will also be useful.
55. Pseudogout:
The clinical manifestations of pseudo gout are similar to
gout.
This disorder is caused by the deposition of calcium
pyrophosphate crystals in joints.
Further serum uric acid concentration is normal in pseudo
gout.
56. Lesch-Nyhan syndrome
This disorder is due to the deficiency of hypoxanthine-
guanine phosphoribosyltransferase (HGPRT) , an enzyme
of purine salvage pathway .
Lesch-nyhan syndrome is a sex-linked metabolic disorder
since the structural gene for HGPRT is located ontlhe X-
chromosome.
It affects only the males and is characterized by excessive
uric acid production (often gouty arthritis).
57. Neurological abnormalities such as mental
retardation, aggressive behavior, learning disability etc.
The patients of this disorder have an irretible urge to bite
their fingers and lips, often causing self-mutilation.
The overpodluction of uric acid in lesch-nyhan syndrome
is explained .
HGPRT deficiency results in the accumulation of PRPP and
decrease in GMP and IMP, ultimatelyleading to increased
synthesis and degradation of purines.
58. The biochemical bases for the neurological symptoms
observed in Lesch-Nyhan syndrome is not clearly
understood.
This may be related to the dependence fo brian on the
salvage pathway for de novo synthesis of purine
nucleotides.
Uric acid is not toxic to the brain, since patients with
severe hyperuricemia (not related to HGPRT deficiency)
do not exhibit any neurological symptoms.
59. Immunodeficiency diseases associated with
purine metabolism
Two different immunodeficiency disorders associated
with the degradation of purine nucleosides are identified.
The enzyme defects are adenosine deaminase and purine
nucleoside phosphorylase, involved in uric acid synthesis.
The deficiency of adenosine deaminase (ADA) causes
severe combined immunodeficiency (SCID) involving T-cell
and usually B-cell dysfunction.
60. It is explained that ADA deficiency results in the
accumulation of dATP which is an inhibitor of
ribonucleotide reductase and therefore DNA synthesis
and cell replication.
The deficienc of purine nucleotide phosphorylase is
associated with impairment; of T-cell function but has no
effect on B-cell function.
Uric acid synthesis is decreased and the tissue levels of
purine nucleosides and nucleotides are higher.
It is believed that dGTP inhibits the development of
normal T-cells