Biosafety level 4 requires the highest level of containment precautions and is required for work with dangerous and exotic agents that can cause severe or fatal disease for which there are no vaccines or treatments. Strict protocols must be followed, including requiring two people to work at all times, full protective clothing and respiratory equipment, and decontamination of all materials. The laboratory facilities for biosafety level 4 include primary containment like class III cabinets and sealed suit laboratories, emergency power, controlled access and air systems, and sterilization of all waste before disposal.

1. BIOSAFETY LEVEL 4Presented by :SheetalNarkarPooja Lad

2. BIOSAFETYThe application of the combination of laboratory practice and procedures, laboratory facilities and safety equipment when working with potentially infectious micro-organisms

3. BIOSAFETY LEVELA biosafety level is the level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed facilityThere are mainly 4 biosafety levels

6. BIOSAFETY LEVEL 4This level is required for work with dangerous and exotic agents that pose a high individual risk of :aerosol-transmitted laboratory infections,

7. agents which cause severe to fatal disease in humans for which vaccines or other treatments are not availableEg. Lassa fever, Ebola hemorrhagic fever, hemorrhagic diseases

8. CODE OF PRACTICESThe two-person rule should apply, whereby no individual ever works alone. This is particularly important if working in a Biosafety Level 4 suit facilityLaboratory protective clothing, head coverings, scrub suits, coveralls, shoe cover etcRespiratory protective equipment

9. CODE OF PRACTICES A complete change of clothing and shoes is required prior to entering and upon exiting the laboratoryPersonnel must be trained in emergency situations like in the event of personnel injury or illness.

10. LABORATORY DESIGN AND FACILITY Primary ContainmentClass III cabinet laboratory

11. Suit laboratoryEmergency PowerUV light and vacuum room

12. Controlled AccessControlled Air System

13. Decontamination of effluentsSterilization of waste and materials

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