The document discusses several water soluble vitamins, including vitamin B complex vitamins, vitamin C, and folate. It focuses on their structures, functions, deficiencies, and food sources. Key points include: thiamine, riboflavin, niacin, pyridoxine, biotin, and folate are all part of the B complex vitamins. They act as coenzymes in various metabolic pathways. Deficiencies can result in conditions like beriberi, pellagra, and anemia. Good dietary sources include meats, vegetables, fruits, and whole grains.
Vitamin C and Vit B1 to B6 by Dr Anurag YadavDr Anurag Yadav
Details related to the Vitamin C and Vitamin B1 to B6. The biochemistry of these water soluble vitamins are explained under all the necessary heading.
Useful for students of MBBS, BDS, BPT, Nursing, BSc, MSc etc
Importance of Vitamins and Minerals for Dairy Cattle. The article written by Mr. Rakesh Kumar, Marketing Director, Growel Agrovet Private Limited, has been published in Dairy Planner magazine, March – 2021 edition.
Vitamin C and Vit B1 to B6 by Dr Anurag YadavDr Anurag Yadav
Details related to the Vitamin C and Vitamin B1 to B6. The biochemistry of these water soluble vitamins are explained under all the necessary heading.
Useful for students of MBBS, BDS, BPT, Nursing, BSc, MSc etc
Importance of Vitamins and Minerals for Dairy Cattle. The article written by Mr. Rakesh Kumar, Marketing Director, Growel Agrovet Private Limited, has been published in Dairy Planner magazine, March – 2021 edition.
Two biologically active forms
Flavin mononucleotide (FMN)
Flavin adenine dinucleotide (FAD)
FAD and FMN are derived from riboFlavin (B2 = 2 ATP)
deficiency:
Cheilosis (inflammation of lips, scaling and fissures at corners of mouth)
Dermatitis
Glossitis (tongue smooth and purplish)
vit B3
Derived from tryptophan
Two biologically active forms
Nicotinamide Adenine Dinucleotide (NAD+)
Nicotinamide Adenine Dinucleotide Phosphate (NADP+)
NAD derived from Niacin (B3 = 3ATP)
Riboflavin,flavoproteins and their clinical applicationsrohini sane
A presentation in lucid-style on Riboflavin (vitamin B2), Flavoproteins and their clinical applications for MBBS , BDS , B Pham and Biotechnology students to facilitate easy leaning.
Basics of animal nutrition
Feeding the rumen microbes
We need:
Certain limiting amino acids (Lys, Met)
In a certain ratio (Lys 3 : 1 Met)
And normal feedstuffs don’t supply this:
Not enough
Not the right ratio
So we need to supplement them
We need RUP
PYRIDOXINE INDUCED PERIPHERAL NEUROPATHY A DETAILED REVIEW.PARUL UNIVERSITY
Vitamin B6 a water-soluble vitamin, also known as pyridoxine that the body needs for several important
functions. It is significant to protein, fat, and carbohydrate metabolism and the creation of red blood cells and
neurotransmitters. Pyridoxine (vitamin B6) is a co-factor in many enzymatic pathways involved in amino acid
metabolism. It is also recommended as a co-factor to improve the conversion of glyoxylic acid into glycine in
ethylene glycol poisoning. Pyridoxine is active in its phosphorylated form of pyridoxal-5-phosphate. Peripheral
neuropathy refers to the conditions that result when nerves that carry messages to and from the brain and spinal
cord from and to the rest of the body are damaged or diseased. Although the family of B vitamins is often nontoxic, high doses of pyridoxine cause peripheral sensory nerve damage. There are some case reports of sensory
neuropathies at doses of less than 500 mg per day in patients taking supplements for months. However, none of
the studies had sensory nerve damage at daily intake below 200 mg pyridoxine per day.Thus, intake of doses
greater than 100-300mg/d or even 50 mg/d used for longer than 6 months would be deemed harmful.
Two biologically active forms
Flavin mononucleotide (FMN)
Flavin adenine dinucleotide (FAD)
FAD and FMN are derived from riboFlavin (B2 = 2 ATP)
deficiency:
Cheilosis (inflammation of lips, scaling and fissures at corners of mouth)
Dermatitis
Glossitis (tongue smooth and purplish)
vit B3
Derived from tryptophan
Two biologically active forms
Nicotinamide Adenine Dinucleotide (NAD+)
Nicotinamide Adenine Dinucleotide Phosphate (NADP+)
NAD derived from Niacin (B3 = 3ATP)
Riboflavin,flavoproteins and their clinical applicationsrohini sane
A presentation in lucid-style on Riboflavin (vitamin B2), Flavoproteins and their clinical applications for MBBS , BDS , B Pham and Biotechnology students to facilitate easy leaning.
Basics of animal nutrition
Feeding the rumen microbes
We need:
Certain limiting amino acids (Lys, Met)
In a certain ratio (Lys 3 : 1 Met)
And normal feedstuffs don’t supply this:
Not enough
Not the right ratio
So we need to supplement them
We need RUP
PYRIDOXINE INDUCED PERIPHERAL NEUROPATHY A DETAILED REVIEW.PARUL UNIVERSITY
Vitamin B6 a water-soluble vitamin, also known as pyridoxine that the body needs for several important
functions. It is significant to protein, fat, and carbohydrate metabolism and the creation of red blood cells and
neurotransmitters. Pyridoxine (vitamin B6) is a co-factor in many enzymatic pathways involved in amino acid
metabolism. It is also recommended as a co-factor to improve the conversion of glyoxylic acid into glycine in
ethylene glycol poisoning. Pyridoxine is active in its phosphorylated form of pyridoxal-5-phosphate. Peripheral
neuropathy refers to the conditions that result when nerves that carry messages to and from the brain and spinal
cord from and to the rest of the body are damaged or diseased. Although the family of B vitamins is often nontoxic, high doses of pyridoxine cause peripheral sensory nerve damage. There are some case reports of sensory
neuropathies at doses of less than 500 mg per day in patients taking supplements for months. However, none of
the studies had sensory nerve damage at daily intake below 200 mg pyridoxine per day.Thus, intake of doses
greater than 100-300mg/d or even 50 mg/d used for longer than 6 months would be deemed harmful.
University Institute of Pharmaceutical Sciences is a flag bearer of excellence in Pharmaceutical education and research in the country. Here is another initiative to make study material available to everyone worldwide. Based on the new PCI guidelines and syllabus here we have a presentation dealing with the vitamins and there deficiencies.
Thank you for reading.
Hope it was of help to you.
UIPS,PU team
Cyclic Peptides Current Status & Future Prospects.pdfDoriaFang
Researchers have made unremitting efforts to optimize peptides in order to improve the bioavailability of peptide drugs. Cyclization of peptides is one of the methods to optimize peptides. Cyclic peptides combine several favorable properties such as good binding affinity, target selectivity and low toxicity that make them an attractive modality for the development of therapeutics.
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...AmalDhivaharS
Obesity is considered to be a crucial health issue leading to some of the disturbing
physical, psychological and social consequences. Obesity refers to the state of being
grossly fat or overweight. Though proper medications have not been framed for it’s
cure, the amount of money and hard work invested for formulating a solution
regarding this affair is massive. Developing natural products from fruits and
vegetables with bioactivities into anti-disease agents has become a hot spot in
research. In a similar case, anthocyanins are found to be extra attractive, as they
have been proved to show anti-obesity effects. Not just considering this water
soluble vacuolar pigment as a medicine, but making it as a part of one’s daily diet
would help reduce the complexity in attempting to lose weight, that has been
gained unnecessarily. Thus, understanding the medicinal avails of anthocyanin and
implementing it as a part of one’s diet would be a more effective strategy for
approaching obesity and related predicaments.
This Presentation Had been made under the following SLOS'
1. Describe the importance of various dietary components and 2. explain importance of dietary fibre
3. Explain nutritional quality of proteins
4. Discuss and explain normal dietary requirements, basal metabolic rate, and thermogenic effect (specific dynamic action, SDA) of food
5.Describe balanced diet in adult, in childhood and in pregnancy for optimal health
6.Describe types and causes of protein energy malnutrition, and its effects
7.Describe causes, effects and health risk associated with obesity
8.Provide dietary advice in diabetes mellitus and coronary heart disease
Similar to Bio water soluble-vitamins_by_dr_shivani_pandey-16-12-14 (20)
astigmatism
AstigmatismAstigmatism Walter Huang, ODWalter Huang, OD Yuanpei UniversityYuanpei University Department of OptometryDepartment of Optometry
2. DefinitionDefinition When parallel rays of light enter the eyeWhen parallel rays of light enter the eye ((with accommodation relaxedwith accommodation relaxed) and do) and do notnot come to a single point focus on or nearcome to a single point focus on or near the retinathe retina
3. OpticsOptics Power in thePower in the horizontalhorizontal plane projects aplane projects a verticalvertical focal line imagefocal line image Power in thePower in the verticalvertical plane projects aplane projects a horizontalhorizontal focal line imagefocal line image
4. OpticsOptics Refraction of light taking place at a toricRefraction of light taking place at a toric surface: the conoid of Sturmsurface: the conoid of Sturm
TONOMETRY • Tonometry is the procedure performed to determine the intraocular pressure (IOP).
3. CLASSIFICATION TONOMETRY DIRECT INDIRECT Indentation Applanation Manometer
4. APPLANATION Contact Non-contact Goldmann Perkins Air-puff Pulse air
5. INDENTATION TONOMETER • It is based on fundamental fact that plunger will indent a soft eye more than hard eye. • The indentation tonometer in current use is that of Schiotz . • It was devised in 1905 and continued to refine it through 1927.
6. PROCEDURE • Patient should be anaesthetising with 4% lignocaine or 0.5% proparacaine. • with the patient in supine position, looking up at a fixation target while examiners separates the lids and lower the tonometer plate to rest on the cornea so that plunger is free to move. •
. Introduction Biomicroscope derives its name from the fact that it enables the practitioner to observe the living tissue of eye under magnification. It not only provides magnified view of every part of eye but also allows quantitative measurements and photography of every part for documentation.
3. • The lamp facilitates an examination which looks at anterior segment, or frontal structures, of the human eye, which includes the –Eyelid –Cornea –Sclera –Conjunctiva –Iris –Aqueous –Natural crystalline lens and –Anterior vitreous.
4. Important historical landmarks De Wecker 1863 devised a portable ophthalmomicroscope . Albert and Greenough 1891,developed a binocular microscope which provided stereoscopic view. Gullstrand ,1911 introduced the illumination system which had for the first time a slit diapharm in it Therefore Gullstrand is credited with the invention of slit lamp.
1. Introduction Gross anatomy Layers Blood supply, drainage and nerve supply
2. INTRODUCTION • Sclera forms posterior 5/6th of external tunic , connective tissue coat of eyeball. • it continues with duramater and cornea • Its whole surface covered by tenon’s capsule • Anteriorly covered by- bulbar conjunctiva • Inner surface lies in contact with choroid • With a potential suprachoroidal space in between
3. Equa THICKNESS OF SCLERA
4. • Thickness varies with individual, with age • Thinner- children, elder, F> M • Thickest posteriorly • Gradually becomes thinner when traced anteriorly • Thin at insertion of extraocular muscle
The pupil is an opening located in the center of the iris that allows light to enter the retina. • Its function is to control the amount of light entering the eye and it does this via contraction (miosis) and dilation (mydriasis) under the influence of the autonomic nervous system
3. • The iris is a contractile structure, consisting mainly of smooth muscle, surrounding the pupil. Light enters the eye through the pupil, and the iris regulates the amount of light by controlling the size of the pupil.
4. The iris contains two groups of smooth muscles: a circular group called the sphincter pupillae. and a radial group called the dilator pupillae.
5. Parasympathetic pathway • First Order – Retina to Pretectal Nucleus in B/S (at level of Superior colliculus) Second Order – Pretectal nucleus to E/W nucleus (bilateral innervation!) Third Order – E/W nucleus to Ciliary Ganglion Fourth Order – Ciliary Ganglion to Sphincter pupillae (via short ciliary nerves) • • •
The tear film constitutes Three layers :- An outermost lipid (oily) layer An aqueous (watery) layer that makes up 90% of the tear film volume; and A mucin layer that coats the corneal surface.
3. To form smooth optical surface on cornea. To keep the surface of cornea & conjunctiva moist It serve as lubricant It transfer oxygen Provide antibacterial action Wash debris out It provides a pathway for WBC in case of injury
4. Functions of lipid layer Retards evaporation of tear film Prevents the overflow of tears
5. Function of Aqueous Layer Flushes, buffers and lubricates the corneal surface Delivers oxygen and other nutrients to the corneal surface Wash out debris Delivers antibacterial enzymes and antibodies such as lysozyme.
6. Functions of Mucin Layer Spreads tears over corneal surface. Protects the cornea against foreign substances . Makes corneal surface smooth by filling in surface irregularities
Introduction Transparent,avascular,watch-glass like structure. Forms 1/6th part of outer fibrous coat (Sclera) It is the major refracting surface of the eye
3. Dimensions + Avg horizontal dia =11.75 mm (ant surface) + Avg vertical dia = 11 mm (ant surface ) + Avg dia (post surface)= 11.5 mm + Thickness(centre) =0.52mm + Thickness(peripheral) = 0.67mm + Radius of curvature (ant surface) = 7.8mm + Radius of curvature (post surface)= 6.5mm + Refractive power (ant surface) = +48D + Refractive power(post surface)= - 5D + R.I = 1.376
4. Histology + Epithelium + Bowman’s membrane + Stroma + Dua’s layer + Descemet’s membrane + Endothelium
5. Epithelium + Made up of stratified squamous epithelium + Thickness - 50-90 um + 5-6 layers of cells + Regenerative, entire epithelial layer is replaced every 6-8 days + Made up of 3 types of cells - basal,wing, flattened cells + Cells are attached by to each other by means of desmosomes & maculi occludents
6. Bowman’s membrane + Acellular,Non regenerative + Made up of condensed collagen fibrils. + Thickness - 12um + Resistant to infection & injury.
LIMBUS… • The limbus forms the border between the transparent cornea and opaque sclera, contains the pathways of aqueous humour outflow, and is the site of surgical incisions for cataract and glaucoma
2. Anatomical Limbus: Circumcorneal transitional zone of the conjunctivocorneal & corneoscleral junction Conjunctivo-corneal junction: • Bulbar conjunctiva is firmly adherent to underlying structures • Substantia propria of the conjunctiva stops here but its epithelium continues with that of the cornea. Sclero-corneal junction: • Transparent corneal lamellae become continuous • With the oblique, circular and opaque fibres of sclera
3. CONTINUE…. • In the area near limbus, the conjunctiva, tenon’s capsule & the episcleral tissue are fused into a dense tissue which is strongly adherent to corneo scleral junction.It is preferred site for obtaining a firm hold of the eyeball during ocular surgery. • The limbus is a common site for the occurrence of corneal epithelial neoplasm. • The Limbus contains radially oriented fibrovascular ridge known as the palisades of Vogt that may harbour a stem cell population. The palisades of Vogt are more common in the superior and inferior quadrants around the eye
Diagnosis, Management, and Surgery by Adam J. Cohen, Michael Mercandetti & Brian G. Brazzo. The dry eye , a practical approach by Sudi Patel & Kenny J Blades. Jack J Kanski’s clinical ophthalmology Clinical Anatomy of the Eye by Richard S. Snell & Michael A. Lemp.
3. It is concerned with the tear formation & transport. Lacrimal passage includes : Lacrimal gland Conjunctival sac Lacrimal puncta Lacrimal canaliculi Lacrimal sac Nasolacrimal duct
4. The following components of the lacrimal apparatus are discussed : Embryology Osteology Secretory system Excretory system Physiology
5. Ectodermal origin Solid epithelial buds(first 2 months) Supero
Extraocular musles(EOM) They are six in number Four recti: Superior rectus Inferior rectus Medial rectus Lateral rectus Two oblique muscles: Superior oblique Inferior oblique
3. SUPERIOR RECTUS MUSCLE . Origin Superior part of common annular tendon of Zinn Course Passes anterolaterally beneath the levator At 23 degrees with the globe ‘s AP axis Pierces Tenon s capsule Insertion into sclera by flat tendinous 10 mm broad insertion 7.7 mm behind sclero-corneal junction. 42 mm long 9 mm wide
4. Nerve supply Sup division of 3rd N Blood Supply Lateral Muscular br. of Ophthalmic A APPLIED: SR loosely bound to LPS muscle. • During SR resection- eyelid may be pulled forward narr owing palpebral fissure • In hypotropia pseudoptosis may be present Origin of SR and MR are closely attached to the dural sheat h of the optic nerve pain during upward & inward movements of the globe in RETROBULBAR NEURITIS
Diseases of sclera
2. anatomy • Sclera posterior 5/6th opaque part of the external fibrous tunic of the eyeball.
3. • outer surface }covered by Tenon's capsule. • anterior part } covered by bulbar conjunctiva.
4. Its inner surface lies in contact with choroid with a potential suprachoroidal space in between
5. Thickness of sclera. • thinner }children and in females Sclera • thickest} posteriorly (1mm) • gradually becomes thin when traced anteriorly. • thinnest } insertion of extraocular muscles (0.3 mm). • Lamina cribrosa is a sieve-like sclera from which fibres of optic nerve pass.
6. Apertures of sclera • Anterior • Anterior ciliary vessels • Middle • four vortex veins (vena verticosae) • Posterior • Optic nerve • Long & short ciliary nerves
7. Layers of sclera sclera episclera Sclera proper Lamina fusca thin, dense vascularised layer of connective tissue fibroblasts, macrophages and lymphocytes avascular structure dense bundles of collagen fibres. innermost blends with suprachoroidal and supraciliary laminae of the uveal tract. brownish in colour presence of pigmented cells.
Main physiologic function of cornea is to act as a major refracting medium, so that a clear retinal image is formed. • Normal corneal transparency is result of • 1.anatomical factor such as uniform and regular arrangement of corneal epithelium, peculiar arrangement of corneal lamella and corneal vascularity 2.Physiological factor [ie] relative state of corneal dehydration.
3. • Therefore, any process which upsets the anatomy or physiology of cornea will cause LOSS OF TRANSPARENCY to some degree.
4. FACTORS AFFECTING CORNEAL TRANSPARENCY • CORNEAL EPITHELIUM &TEAR FLIM • ARRANGEMENT OF STROMAL LAMELLA • CORNEAL VASCULARIZATION • CORNEAL HYDRATION • CELLULAR FACTORS AFFECTING TRANSPARENCY
CONJUNCTIVA: ANATOMY , PHYSIOLOGY, SYMPTOMATOLOGY AND CLASSIFICATION Pranay Shinde DNB Resident Deen Dayal Upadhyay Hospital,New Delhi
2. ANATOMY It is the mucous membrane covering the under surface of the lids and anterior part of the eyeball upto the cornea.
3. Parts of conjunctiva • Palpebral; covering the lids—firmly adherent. • Forniceal; covering the fornices—loose—thrown into folds. • Bulbar; covering the eyeball—loosely attached except at limbus.
4. Palpebral conjunctiva • Subtarsal sulcus 2mm from posterior edge of the lid margin. • Richly vascular. • Extremely thin. • Strongly bound to the tarsal plate.
5. Palpebral conjunctiva is subdivided into three parts: 1)Marginal 2)Tarsal 3)Orbital
6. Conjunctival fornices • Transitional region between palpebral and bulbar conjunctivae. • Superior fornix 10 mm from limbus. • Inferior fornix 8 mm from limbus. • Lateral fornix 14mm from limbus. • Medially absent. • Ducts of lacrimal glands open into lateral part of superior fornix.
q Colour Vision Deficiency Presented by : Optometrist (intern) Asma Al-Jroudi Saudi Arabia, Riyadh, King Abdulaziz University Hospital 30 Dec 14
2. • What Is Color Vision Deficiency? • Causes Of Color Vision Deficiency • Types Of Color Vision Deficiency • Tretments Of Color Vision Deficiency • Ishihara’s Test • Conclousion
3. What is Colour Blindness? • Color blindness, or color vision deficiency, is the inability or decreased ability to see color, or perceive color differences, under normal lighting conditions. •This condition results from an absence of color- sensitive pigment in the cone cells of the retina, the nerve layer at the back of the eye.
4. What is Colour Blindness? • Cones are the coulored light receptors in back of the eye: Red light receptors, Blue light receptors and Green light receptors. • Colour blindness occurs when one or more of the cone types are defected.
5. Causes of Color Blindness • Genetic: Many more men are affected than women. • Acquired : Chronic illness, Accidents, Medications and Age.
ANATOMY & PHYSIOLOGY Lecturer: Tatyana V. Ryazantseva
2. Outer eye: Eyelids The eyelids fulfill two main functions: protection of the eyeball secretion, distribution and drainage of tears
3. Lid movement The levator extends from an attachment at the orbital apex to attachments at the tarsal plate and skin. ● The lids are securely attached at either end to the bony orbital margin by the medial and lateral palpebral ligaments. Trauma to the medial ligament causes the lid to flop forward and laterally, impairing function and cosmesis.
4. Innervation - Sensory innervation is from the trigeminal (fifth) cranial nerve, via the ophthalmic division (upper lid) and maxillary division (lower lid). - The orbicularis oculi is innervated by the facial (seventh) nerve. - The levator muscle in the upper lid is supplied by the oculomotor (third) nerve.
5. Blood supply and lymphatics The eyelids are supplied by an extensive network of blood vessels which form an anastomosis between branches derived from the external carotid artery via the face and from the internal carotid artery via the orbit.
6. Blood supply and lymphatics Lymphatic fluid drains into the preauricular and submandibular nodes. Preauricular lymphadenopathy is a useful sign of infective eyelid swelling (especially viral).
Anatomy and Physiology of Aqueous Humor Sumit Singh Maharjan
2. Anatomy
3. Angle of anterior chamber
4. Angle of the Anterior chamber
5. Gonioscopic grading of Angle
6. Aqueous Outflow system
7. Trabecular meshwork
8. Functions of Aqueous Humor • Maintenance of Intraocular pressure • Metabolic role cornea lens vitreous and retina • Optical function • Clearing function
9. Physicochemical properties • volume: 0.31ml (0.25ml in Ant. Chamber and 0.06 in post chamber) • Refractive index: 1.336 • Density: slightly greater than water, its viscocity is 1.025-1.040 • Osmotic pressure: slightly hyperosmotic to plasma by 3-5mosm/l • PH: 7.2 • Rate of formation: 2-2.5microliter/min
10. Biochemical composition • Water: 99.9% • Proteins: 5-16mg/100ml • Amino acids: aqueous/plasma concentration varies from 0.08-3.14 • Non colloidal constituents: conc. of ascorbate, pyruvate, lactate in higher am
Vitreous humour
1. Vitreous Humour
2. General features Vitreous humour is an inert ,transparent , colourless, jellylike, hydrophilic gel that serves the optical functions and also acts as important supporting structures for the eyeball. The vitreous cavity is bounded by anteriorly by the lens and ciliary body and posteriorly by the retina Its weighs nearly 4g Vitreous is an extacellular material composed of approximately 99 per cent water
3. Structure The vitreous body is the largest and simplest connective tissue present as a single piece in the human body Divided into three parts- 1. The hyaloid layer or membrane 2. The cortical vitreous and 3. The medullary vitreous
Vitamins all
1. Vitamins. Definition - Organic compound required in small amounts. Vitamin A Vitamin B1, B2, B3, B5, B6, B7, B9, B12 Vitamin D Vitamin E Vitamin K A few wordsabout each.
2. Sourcesin diet - Many plants(photoreceptors), also meat, especially liver. Fat soluble, so you can get too much, or too littleif absorption isaproblem. Vitamin A - Retinol Retinol (vitamin A) Someuses: Vision (11-cis-retinol bound to rhodopsin detectslight in our eyes). Regulating genetranscription (retinoic acid receptorson cell nuclei arepart of a system for regulating transcription of mRNAsfor anumber of genes).
Tear film
1. TEAR FILM
2. The outer most layer of the cornea. It is the exposed part of the eyeball. FUNCTION It provide smooth optical surface It serves to keep the surface of cornea and conjunctiva moist. It serves as a lubricant for the preocular surface and lids It transfer oxygen from the air to the cornea Prevent infection due to the presence of antibacterial substance like lysozymes,and other protein. It wash away debris and irritants Provides pathway to WBC in case of injury.
3. LAYERS OF TEAR FILM It consist of three layers: 1.Lipid layer 2.Aqueous layer 3.Mucoid layer 1.LIPID LAYER
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69. Dr. Shivani Pandey, Department of Biochemistry, K.G. Medical
University, Lucknow
Pyridoxine
70. PYRIDOXINE (vitamin B6)
Pyridoxal (PL) Pyridoxamine (PM)
Pyridoxine or
Pyridoxol (PN)
Each of these forms can be phosphorylated at position 5 to form: PLP, PMP & PNP
Dr. Shivani Pandey, Department of Biochemistry
71. Active form
Pyridoxal phosphate (PLP)
• PLP and PL account for 90% of the
total B6 in the blood.
• In the blood B6 is transported both
in the plasma and the RBCs.
• In the blood PLP is hydrolyzed to
PL because only free PL gets inside
the cells.
• In muscle and other tissues, PL is
converted back to PLP by a reversible
reaction with the help of alkaline
phosphatase and pyridoxal kinase.
• Active functional form is pyridoxal
phosphate (PLP) and pyridoxamine
phosphate (PMP).
• For absorption, the “phosphorylated”
form must be hydrolyzed to
“dephosphorylated” form by the enzyme
alkaline phosphatase in the intestine.
• In the portal vein Vit B6 is present as PL,
PM, PN.
• In the liver they are converted back to
phosphorylated forms. This conversion is
catalyzed by the ATP requiring enzyme,
pyridoxal kinase.
Dr. Shivani Pandey, Department of Biochemistry
72. Functions
FUNCTIONS: B6 is involved in:
Amino acid metabolism
Transamination reactions required for the synthesis and catabolism
of the amino acids.
Decarboxylation reactions.
Breakdown of glycogen Glycogenolysis (cofactor for glycogen phosphorylase).
80-90% of body vit B6 is present in the muscles, most of it in PLP
(coenzyme) form bound to glycogen phosphorylase. Only 1 mol or
less is present in the blood,
Synthesis of epinephrine (adrenaline) and norepinephrine (noradrenaline)
Synthesis of niacin (vitamin B3) from the amino acid tryptophan.
Dr. Shivani Pandey, Department of Biochemistry
73. Covalent bonds of -amino acids made labile by their
binding to PLP-containing enzyme
In the reactions of amino acid metabolism, the formyl (CHO) group of PLP
condenses with -NH2 group of an amino acid and forms a Schiffs base. This
linkage weakens or labilizes all the bounds around the -carbon of the amino acid.
The specific bond of an amino acid that is broken depends on the particular
enzyme to which PLP is attached.
Dr. Shivani Pandey, Department of Biochemistry
75. Deficiency
Food sources:
In animal foods major forms are PL and and PM along with their phosphorylated forms.
In plants PN.
Bananas, beans, lentils, walnuts, salmon, chicken, beef, whole grain breads and cereals,
soybeans, liver, eggs, dairy products are excellent sources.
Requirements:
The requirement for vitamin B6 in the diet is proportional to the level of protein
consumption ranging from 1.4 - 2.0 mg/day for a normal adult.
During pregnancy and lactation the requirement for vitamin B6 increases
approximately 0.6 mg/day.
TOXICITIES:
Megadoses of B6 (daily doses of >500mg) are used to treat pms symptoms. They can
cause neurotoxicity and photosensitivity in some individuals.
Deficiencies: are rare and usually are related to an overall deficiency of all the
B-complex vitamins.
Certain drugs form complexes with PL and PLP
Penicillamine (used to treat rheumatoid arthritis and cystinurias).
Isoniazid (the hydrazide derivative of isonicotinic acid) is the primary drug for
chemotherapy of tuberculosis.
Dr. Shivani Pandey, Department of Biochemistry
77. BIOTIN
Biotin is relatively small, bicyclic (two-ring) compound
formed from a tetrahydrothiophene (thiophene) ring ,
and a second ring, which contains a ureido group.
The thiophene ring also has a valeric acid side chain.
Although eight different stereoisomers of biotin exist, only
one stereoisomer is found naturally and to have biological
activity as a coenzyme. It is called d-(+)-biotin, D-biotin or
simply biotin.
It is
sometimes
called vitamin
H and also
coenzyme R.
Dr. Shivani Pandey, Department of Biochemistry
78. Holocarboxylase
In humans, the four holocarboxylases are : acetyl-CoA carboxylase,
propionyl-CoA carboxylase, pyruvate carboxylase and beta-
methylcrotonyl-CoA carboxylase. Biotin is chemically bonded in each of these
enzymes via an amide linkage between the carboxyl group of the valeric acid
side-chain in biotin and the epsilon-amino group of the lysine residue in the
apocarboxylase.
The enzyme that catalyzes the formation of this covalent bond is called
holocarboxylase synthetase. Dr. Shivani Pandey, Department of Biochemistry
79. Biotin Cycle
Biotin cycle: the chain of chemical reactions involved in the use and reuse of the
vitamin biotin. One important role of biotinidase is:
1. To separate or free biotin from proteins to which it is bound in foods. Biotin in its
free form can then be used by the body.
2. Biotinidase lets the body recycle or reuse the biotin over and over again so that we
do not need to consume large amounts of this vitamin in our diets.
•Within cells, the carboxylases
(pyruvate carboxylase, acetyl-CoA
carboxylase, methycrotonyl-CoA
carboxylase, propionyl-CoA
carboxylase) are biotinylated via
holocarboxylase synthetase. Biotin
and apocarboxylases are the
substrates. ATP and magnesium also
participate in the reaction.
Biotinidase deficiency is a treatable,
inherited metabolic disorder in which the
body cannot process the vitamin biotin in a
normal manner.
Dr. Shivani Pandey, Department of Biochemistry
80. Functions
Coenzyme involving CO2 transfer
acetyl CoA to malonyl CoA
Propionyl CoA to methylmalonyl CoA
Pyruvate to oxaloacetate
Deficiency
Fatty liver and kidney syndrome, a fatal metabolic
disorder in chicks and turkey poults
Raw egg white (avidin) can block absorption
Dr. Shivani Pandey, Department of Biochemistry
81. Biotin (functions)
Coenzyme for several reactions involving CO2 fixation into various
compounds e.g.
Pyruvate to oxaloacetate
(pyruvate carboxylase)
Propionyl CoA to methylmalonyl CoA
(propionyl CoA carboxylase)
Acetyl CoA to malonyl CoA
(acetyl CoA carboxylase)
- initial step in de novo fatty
acid synthesis.
Dr. Shivani Pandey, Department of Biochemistry
82. Deficiency symptoms
Rare because of widespread distribution in plant and
animal food and significant lower gut synthesis.
Sources
Yeast, rice, soybeans, peanuts, fish (herring and
mackerel), mushrooms and bananas, safflower meal,
liver and milk are rich sources.
Can be induced by eating raw egg white
The fact is that nature created the egg in such a way that
its yolk is very rich in biotin. One of the highest
concentration in nature. Eat the egg whole together with the
egg white and you will be fine.
Egg whites contain a glycoprotein called "avidin" which
binds biotin - one of the B vitamins - very effectively. The
cooking process deactivates the avidin in the egg, much the
same way it deactivates every other protein in the egg
white.
Biotin deficiency is chief cause of fatty liver and kidney
syndrome.
This baby developed
severe biotin deficiency
during intravenous feeding
without biotin.
Aajonus Vonderplanitz,
in his book “We Want to
live” is a strong proponent
of raw eggs.
Dr. Shivani Pandey, Department of Biochemistry
83. How Biotin Works
1- Biotin carrier protein
2- Biotin carboxylase
3- Transcarboxylase
Dr. Shivani Pandey, Department of Biochemistry
85. FOLIC ACID (folacin)
• Folacin includes several derivatives of folic acid
(monopteroylglutamic acid).
• Folic acid is obtained primarily from yeasts and leafy vegetables as
well as animal liver. Animals cannot synthesize PABA nor attach
glutamate residues to pteroic acid, thus, requiring folate intake in the
diet.
“Microorganisms
Only can
synthesize Folacin”
Sulphonamides
and antibiotics
block PABA
incorporation
by competitive
inhibition
Dr. Shivani Pandey, Department of Biochemistry
86. Structure
Folic acid exists in a polyglutamate form. Intestinal mucosal cells
remove some of the glutamate residues through the action of the
lysosomal enzyme, conjugase.
Dr. Shivani Pandey, Department of Biochemistry
87. Humans and microorganisms:
Folic acid is reduced within cells
(principally in the liver where it is
stored) to tetrahydrofolate (THF or
H4folate) through the action of
folate reductase [or
dihydrofolate reductase (DHFR) ]
which is an NADPH-requiring
enzyme.
Active functional form is:
Tetrahydrafolic acid
(THF).
Dr. Shivani Pandey, Department of Biochemistry
88. Active center (N5 and N10)
Dr. Shivani Pandey, Department of Biochemistry
89. • Active center of tetrahydrofolate (THF). The N5 position is the site of
attachment of methyl and formimino groups, the N10 the site for
attachment of formyl group and that both N5 and N10 bridge the
methylene and methenyl groups.
Dr. Shivani Pandey, Department of Biochemistry
90. Folate conversions
Carrier of one-carbon (e.g. methyl) groups
The one-carbon units are added to, or removed from, metabolites such as:
• histidine
• serine
• methionine
• purines.
Dr. Shivani Pandey, Department of Biochemistry
91. Functions
• Role of N5,N10-methylene-THF in dTMP
synthesis is the most metabolically
significant function for this vitamin.
• Vitamin B12 and N5-methyl-THF in the
conversion of homocysteine to
methionine is important in helping cells to
regenerate needed THF.
Dr. Shivani Pandey, Department of Biochemistry
92. Participation of H4folate in dTMP synthesis
______Deoxyuridine______________ ________Deoxythymidine
____Monophosphate (dUMP)_______________Monophosphate (dTMP)_______
Dr. Shivani Pandey, Department of Biochemistry
94. Deficiency symptoms
• Identical to those for vitamin B12 deficiency:
• Effect of folate deficiency on cellular processes
is upon DNA synthesis.
– Impairment in dTMP synthesis and purine synthesis
– Cell cycle arrest in S-phase of rapidly proliferating cells, especially
hematopoietic cells.
• The result is megaloblastic leukemia as for vitamin B12 deficiency.
– The inability to synthesize DNA during erythrocyte maturation leads to
abnormally large erythrocytes termed macrocytic anemia.
• Deficiency during pregnancy can cause neural tube defects such as
spina bifidia.
Deficiency is rare due to the adequate presence of folate in food.
Deficiency can occur when there is:
1. Poor dietary habits as those of chronic alcoholics.
2. Impaired absorption or metabolism
• Certain drugs such as anticonvulsants and oral contraceptives and
methotrxate can impair the absorption of folate.
3. An increased demand for the vitamin.
• Pregnancy
• Folate will nearly double by the third trimester of pregnancy.
Dr. Shivani Pandey, Department of Biochemistry
95. What are neural tube defects?
• neural tube defects (NTDs) are birth defects
that occur early in pregnancy
– often before a woman knows she is pregnant
• neural tube grows to become baby’s spinal
cord, spine, brain and skull
Dr. Shivani Pandey, Department of Biochemistry
96. The neural tube forms in the embryo and
then closes (between the 2nd and 4th week of
gestation)
A neural tube defect occurs when the neural
tube fails to close properly
The two most common NTDs are
anencephaly and spina bifida
Dr. Shivani Pandey, Department of Biochemistry
98. Which NTDS are most
common?
• Spina Bifida
– a condition that results when
the lower part of the neural
tube fails to develop properly
• Anencephaly
– a fatal condition in which the
upper end of the neural tube
fails to close
Occult
a
Meningocele
Myelomeningocele
Dr. Shivani Pandey, Department of Biochemistry
100. How Can NTDs be Prevented?
• All women of childbearing age should receive 0.4
mg (400 micrograms) of folic acid daily prior to
conception of planned or unplanned pregnancies
and continue thru 1st trimester
• Women with a history of NTD and should receive
daily supplementation of 4 mg (4000 micrograms)
of folic acid starting three months prior to
conception and continuing thru the 1st trimester
Dr. Shivani Pandey, Department of Biochemistry
102. VITAMIN B12 (cobalamin)
• Vitamin B12, is also called cobalamin,
cyanocobalamin and hydroxycobalamin.
• It is built from :
1. A nucleotide and
2. A complex tetrapyrrol ring structure
(corrin ring)
3. A cobalt ion in the center.
4. A R- group
• When R is cyanide (CN), vitamin B12
takes the form of cyanocobalamin.
• In hydroxycobalamin, R equals the
hydroxyl group (-OH).
• In the coenzyme forms of vitamin B12,
– R equals an adenosyl group in
adenosylcobalamin.
– R equals a methyl (-CH3) group in
methylcobalamin.
• Vitamin B12 is synthesized exclusively by
microorganisms (bacteria, fungi and algae)
and not by animals and is found in the liver
of animals bound to protein as
methycobalamin or 5'-
deoxyadenosylcobalamin.
Dr. Shivani Pandey, Department of Biochemistry
103. • Known as the "red" vitamin because it
exists as a dark red crystalline compound,
Vitamin B12 is unique in that it is the only
vitamin to contain cobalt (Co3+) metal ion,
which, gives it the red color.
• The vitamin must be hydrolyzed from
protein in order to be active.
• Intrinsic factor, a protein secreted by
parietal cells of the stomach, carries it to the
ileum where it is absorbed.
• It is transported to the liver and other
tissues in the blood bound to
transcobalamin II.
• It is stored in the liver attached to
transcobalamin I.
– It is released into the cell as
Hydroxocobalamin (see the next slide)
• In the cytosol it is converted to
methylcobalamin.
• Or it can enter mitochondria and be
converted to 5’-deoxyadenosyl
cobalamin.
Dorothy Crowfoot Hodgkin
(1910-1994)
Dr. Stadtman in her lab
Dr. Shivani Pandey, Department of Biochemistry
104. In the cytosol
In mitochondria
Dr. Shivani Pandey, Department of Biochemistry
105. Functions
• Only two reactions in the body require vitamin B12 as a
cofactor:
1. During the catabolism of fatty acids with an odd number of
carbon atoms and the amino acids valine, isoleucine and
threonine the resultant propionyl-CoA is converted to
succinyl-CoA for oxidation in the TCA cycle.
– methylmalonyl-CoA mutase, requires vitamin B12 as a cofactor in
the conversion of methylmalonyl-CoA to succinyl-CoA.
– 5'-deoxyadenosine derivative of cobalamin is required for this
reaction
2. The second reaction catalyzed by methionine synthase
converts homocysteine to methionine
– This reaction results in the transfer of the methyl group from N5-
methyltetrahydrofolate to hydroxycobalamin generating
tetrahydrofolate and methylcobalamin during the process of the
conversion.
Dr. Shivani Pandey, Department of Biochemistry
107. Deficiency symptoms
• Pernicious anemia in humans (inability to absorb B12
because of lack of gastric intrinsic factor).
• Neurological disorders due to progressive demyelination
of nerve cells.
– This results from increase in methylmalonyl-CoA.
– Methylmalonyl-CoA is a competitive inhibitor of malonyl-CoA in
fatty acid biosynthesis.
– Can substitute malonyl-CoA in any fatty acid biosynthesis and
create branched-chain fatty acid altering the architecture of
normal membrane structure of nerve cells.
• Sources
– Synthesized only by microorganisms, so traces only are present
in plants; liver is a rich source.
– B12 is found in organ and muscle meats, fish, shellfish, dairy
products, eggs and in fortified foods like breakfast cereals.
Dr. Shivani Pandey, Department of Biochemistry