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Technical Seminar-2004 
BBIIOOSSEENNSSOORRSS 
Under the guidance of 
Mr. Vivek sharma 
Presented By 
Pramoda Padhy 
Roll no-(EI200117092) 
NATIONAL INSTITUTE OF SCIENCE & TECHNOLOGY 
Palur Hills,Berhampur-761008,Orissa,India 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
IINNTTRROODDUUCCTTIIOONN 
• What Is a Biosensor? 
• Biosensor = bioreceptor + transducer. 
• The bioreceptor is a biomolecule that recognizes 
the target analyte whereas the transducer 
converts the recognition event into a measurable 
signal. 
• Enzyme is a Bioreceptor 
• Requirements for Sensors 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Need for Biosensor 
• Diagnostic Market 
The current climate of prevention the need for detection 
at increasingly lower limits is increasing in many diverse 
areas 
• Clinical Testing 
clinical testing is one of the biggest diagnostic markets 
clinical testing products market in excess of 4000 million US$ 
in the 1990s 
• Other Markets 
The medical arena (Technical Insights Inc.) with 
veterinary and agricultural applications 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
• Specificity 
With biosensors, it is possible to measure specific 
analytes with great accuracy. 
• Speed 
analyte tracers or catalytic products can be directly 
and instantaneously measured 
• Simplicity 
receptor and transducer are integrated into one 
single sensor& the measurement of target analytes without 
using reagents is possible 
• Continuous monitoring capability 
Biosensors regenerate and reuse the immobilized 
biological recognition element 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Applications of Biosensor 
• Health Care 
– Measurement of Metabolites 
– Market Potential 
– Diabetes 
– Insulin Therapy 
– Artificial Pancreas 
• Industrial Process Control 
– Bioreactor Control 
• Military Application 
• Environmental Monitoring 
– Air and Water Monitoring 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Bioreceptor Molecules 
• Enzymes 
Enzymes act as catalysts for biochemical reactions 
occurring in the cell 
• Antibody 
Antibodies constitute about 20% of the total plasma 
protein and are collectively called immunoglobulin 
• Receptor Protein 
Receptor proteins having specific affinity for 
hormones, antibodies, enzymes 
• Other Possibilities 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Transducers 
• Conventional Transducers 
• Piezoelectric Transducers 
• Coniductimetry 
• Capacitance Measurement 
• Thermometry 
• Enzyme Thermistor 
• FET as a Transducer 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Growth of Biosensor 
• Biosensor Configurations 
• Discriminative Membrane 
• Sensitivity Requirements 
• Immunoassay 
• DNA Probe 
• Evolution of Biosensors 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Receptors 
• Receptors at Membrane 
Biological receptors are protein molecules most of 
them are bound to the cell membrane 
• Hormone Receptors 
Many hormones released into the blood do not 
penetrate the cell membrane but react with specific 
receptors at the cell surface 
• Smell Receptor 
taste and olfactory receptors are typical examples of 
this biospecific recognition process 
• Light Receptor 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Types of biosensor 
• Glucose Biosensor 
• Amperometric Biosensor 
Glucose biosensor using glucose oxidase is used as an example 
• Potentiometer Biosensor 
Examples of Potentiometric Biosensor 
Penicillin biosensor 
Glucose biosensor 
Urea biosensor 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Design Variables 
• Immobilization Methods 
(1) adsorption 
(2) entrapment 
(3) covalent coupling 
(4) cross-linking 
• Change in Conformation 
• Change in Microenvironment 
• Non-Uniform Distribution 
• Reaction and Diffusion 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
Future Prospects 
• Data Processing and Pattern 
Recognition 
• Micro Instrument 
• Molecular Electronics 
• Multi-Disciplinary Nature 
Presented by :- Pramoda Padhy (EI200117092)
Technical Seminar-2004 
CONCLUSION 
Biosensors consist of bio-recognition systems, typically 
enzymes or binding proteins, such as antibodies, 
immobilized onto the surface of physico-chemical 
transducers 
Disadvantages 
• They cannot be steam sterilized 
• They react with the product 
• And are oversensitive 
Presented by :- Pramoda Padhy (EI200117092)

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Bio sensors ei

  • 1. Technical Seminar-2004 BBIIOOSSEENNSSOORRSS Under the guidance of Mr. Vivek sharma Presented By Pramoda Padhy Roll no-(EI200117092) NATIONAL INSTITUTE OF SCIENCE & TECHNOLOGY Palur Hills,Berhampur-761008,Orissa,India Presented by :- Pramoda Padhy (EI200117092)
  • 2. Technical Seminar-2004 IINNTTRROODDUUCCTTIIOONN • What Is a Biosensor? • Biosensor = bioreceptor + transducer. • The bioreceptor is a biomolecule that recognizes the target analyte whereas the transducer converts the recognition event into a measurable signal. • Enzyme is a Bioreceptor • Requirements for Sensors Presented by :- Pramoda Padhy (EI200117092)
  • 3. Technical Seminar-2004 Need for Biosensor • Diagnostic Market The current climate of prevention the need for detection at increasingly lower limits is increasing in many diverse areas • Clinical Testing clinical testing is one of the biggest diagnostic markets clinical testing products market in excess of 4000 million US$ in the 1990s • Other Markets The medical arena (Technical Insights Inc.) with veterinary and agricultural applications Presented by :- Pramoda Padhy (EI200117092)
  • 4. Technical Seminar-2004 • Specificity With biosensors, it is possible to measure specific analytes with great accuracy. • Speed analyte tracers or catalytic products can be directly and instantaneously measured • Simplicity receptor and transducer are integrated into one single sensor& the measurement of target analytes without using reagents is possible • Continuous monitoring capability Biosensors regenerate and reuse the immobilized biological recognition element Presented by :- Pramoda Padhy (EI200117092)
  • 5. Technical Seminar-2004 Applications of Biosensor • Health Care – Measurement of Metabolites – Market Potential – Diabetes – Insulin Therapy – Artificial Pancreas • Industrial Process Control – Bioreactor Control • Military Application • Environmental Monitoring – Air and Water Monitoring Presented by :- Pramoda Padhy (EI200117092)
  • 6. Technical Seminar-2004 Bioreceptor Molecules • Enzymes Enzymes act as catalysts for biochemical reactions occurring in the cell • Antibody Antibodies constitute about 20% of the total plasma protein and are collectively called immunoglobulin • Receptor Protein Receptor proteins having specific affinity for hormones, antibodies, enzymes • Other Possibilities Presented by :- Pramoda Padhy (EI200117092)
  • 7. Technical Seminar-2004 Transducers • Conventional Transducers • Piezoelectric Transducers • Coniductimetry • Capacitance Measurement • Thermometry • Enzyme Thermistor • FET as a Transducer Presented by :- Pramoda Padhy (EI200117092)
  • 8. Technical Seminar-2004 Growth of Biosensor • Biosensor Configurations • Discriminative Membrane • Sensitivity Requirements • Immunoassay • DNA Probe • Evolution of Biosensors Presented by :- Pramoda Padhy (EI200117092)
  • 9. Technical Seminar-2004 Receptors • Receptors at Membrane Biological receptors are protein molecules most of them are bound to the cell membrane • Hormone Receptors Many hormones released into the blood do not penetrate the cell membrane but react with specific receptors at the cell surface • Smell Receptor taste and olfactory receptors are typical examples of this biospecific recognition process • Light Receptor Presented by :- Pramoda Padhy (EI200117092)
  • 10. Technical Seminar-2004 Types of biosensor • Glucose Biosensor • Amperometric Biosensor Glucose biosensor using glucose oxidase is used as an example • Potentiometer Biosensor Examples of Potentiometric Biosensor Penicillin biosensor Glucose biosensor Urea biosensor Presented by :- Pramoda Padhy (EI200117092)
  • 11. Technical Seminar-2004 Design Variables • Immobilization Methods (1) adsorption (2) entrapment (3) covalent coupling (4) cross-linking • Change in Conformation • Change in Microenvironment • Non-Uniform Distribution • Reaction and Diffusion Presented by :- Pramoda Padhy (EI200117092)
  • 12. Technical Seminar-2004 Future Prospects • Data Processing and Pattern Recognition • Micro Instrument • Molecular Electronics • Multi-Disciplinary Nature Presented by :- Pramoda Padhy (EI200117092)
  • 13. Technical Seminar-2004 CONCLUSION Biosensors consist of bio-recognition systems, typically enzymes or binding proteins, such as antibodies, immobilized onto the surface of physico-chemical transducers Disadvantages • They cannot be steam sterilized • They react with the product • And are oversensitive Presented by :- Pramoda Padhy (EI200117092)