SlideShare a Scribd company logo
Batch and Continuous Sterilization
Sterilization of Media for Fermentation
Dr. Pavan J Kundur
Assistant Professor
P C Jabin Science College
Hubballi
 Batch Sterilization is the reduction of contaminant
organisms through the heating of a vessel.
The entire volume of media is sterilized at once through
the use of thermal or radiation techniques.
When running a thermal batch sterilization, a system goes
through 3 steps: heating, holding, and cooling.
Heating requires the addition of energy throughout the
entire medium volume.
This can be done by adding heat through a jacket on the
vessel.
The temperature is increased until it reaches the
sterilization temperature where it is held for a set period
of time.
During this phase, most of the unwanted microorganisms
are destroyed.
Finally, the system is cooled to bring the sterile media
back to the desired temperature.
For radiation sterilization, the process is similar to above,
although it uses radiation intensity instead of heat.
In order to sterilize a batch, calculate the total area
underneath the curve. Therefore, model death using first
order kinetics and integrate as seen above. This will yield a
temperature and the corresponding duration of time needed
to sterilize the media.
Advantages:
Most widely used technique
Simple operation
No additional materials are added to the media itself
Disadvantages:
More expensive heat requirements than continuous
sterilization
Best results occur in well-mixed closed vessels
Continuous Sterilization
 Continuous sterilization is the rapid transfer of heat to medium through steam
condensate without the use of a heat exchanger.
 Once the media is in a holding loop, steam is injected to the system via a nozzle.
The medium stays in this loop for a predetermined holding time until the entire
medium is sterile.
 This is more efficient than batch sterilization because instead of expending energy
to heat, hold, and cool the entire system, small portions of the inlet streams are
heated at a time.
 By looping sterile media tubes (which are at higher temperatures) past inlet tubes,
the difference in temperature is used to help heat the unsterile medium.
 So instead of having a cold-water stream cool the sterile media, the lower
temperature unsterile media stream absorbs heat from the warm stream, cooling the
sterile media.
 Finally, the sterile media is flash cooled through an expansion valve to adjust the
temperature to meet process parameters.
Advantages:
Uniform steam requirements throughout the duration of the
sterilization
Simplified process control
Shorter sterilization time means less thermal degradation of
medium
Disadvantages:
High demand for steam in a shorter period of time than batch
Concentration of media becomes dilute due to steam
condensation
Since steam is actually dispersed in media, steam must be
clean to avoid contamination
Advantages of Continuous
Sterilization process
over Batch Sterilization
 Better protection of medium value .
 Batch sterilization wastes energy and can
overcook the medium
 Batch sterilization uses steam or direct heat to
elevate the temperature. Both the heat and the
cooling water are spent with no opportunity for
energy recovery.
 temperature in batch sterilization can not increased
beyond 121°c as if the temperature is increased, the
heating & cooling period increases .
 hence during sterilization the nutrient media will be
degraded .
 Continuous Sterilization is the method of choice .
 In Continuous Sterilization , the medium to be sterilized
is dealt in small increments &
 hence there is shorter heating & cooling periods.
 much higher temperature can be utilized , thus reducing
holding time.
 therefore, in continuous sterilization nutrient
degradation is subsequently less as compared to batch
sterilization .
 This is more efficient than batch sterilization because
instead of expending energy to heat, hold, and cool the
entire system, small portions of the inlet streams are
heated at a time
 Most of the sterilization in the continuous
sterilization process may occurs in the
holding
The holding section is a long pipe,
λhold =
L
uav
length of the pipe
average fluid velocity
in the pipe
 High steam utilization capacity. As compared to batch
sterilization
 The heat can be fully utilized during sterilizing process
and
 therefore, great amount of water and steam can be saved
as well as energy consumption is decreased substantially.
 It can averagely save about 30% of steam and 40% of
cooling water throughout the sterilization process if
Continuous Sterilization Technology is applied.
 Easy Scaling-up of the process .
 On scaling up the fermentation it was very difficult to
attain the correct sterilization conditions using the batch
regime .
 Continuous sterilization using direct steam injection
allowed the design of a precise process producing sterile
medium with the required degree of interaction b/w the
ingredients .
The temperature profile of the medium allowing an easy
estimate of the Del factor (∆) required. This makes
scale-up very simple.
 Easier to automate the process
 The Continuous Sterilizer is not a pressure vessel and
is easy to automate the whole process .
 hence improves the quality of sterilization & reduce
labor intensive
 Reduces manpower, machinery replacement and
maintenance
 Reduction of sterilization cycle time
Thank You

More Related Content

What's hot

Fluidised Bed Bioreactor
Fluidised Bed BioreactorFluidised Bed Bioreactor
Fluidised Bed Bioreactor
Trishna Das
 
Media for industrial fermentation
Media for industrial fermentationMedia for industrial fermentation
Media for industrial fermentation
NithyaNandapal
 
Batch, fedbatch and continuous fermentation
Batch, fedbatch and continuous fermentationBatch, fedbatch and continuous fermentation
Batch, fedbatch and continuous fermentation
Dhanya K C
 
FERMENTERS( BIOREACTORS) AND THEIR TYPES
FERMENTERS( BIOREACTORS) AND THEIR TYPESFERMENTERS( BIOREACTORS) AND THEIR TYPES
FERMENTERS( BIOREACTORS) AND THEIR TYPES
AYESHA KABEER
 
Stirred tank bioreactor
Stirred tank bioreactorStirred tank bioreactor
Stirred tank bioreactor
Nizam Ashraf
 
Fermentor
Fermentor   Fermentor
Fermentor
Rajatsingh
 
strain improvement techniques
strain improvement techniquesstrain improvement techniques
strain improvement techniques
jeeva raj
 
Media formulation
Media formulationMedia formulation
Media formulation
eswar1810
 
Aeration and agitation
Aeration and agitationAeration and agitation
Aeration and agitation
eswar1810
 
Downstream processing - industrial microbiology
Downstream processing - industrial microbiology Downstream processing - industrial microbiology
Downstream processing - industrial microbiology
Kiran Kumar
 
Types of Bioreactors / Fermenters
Types of Bioreactors / FermentersTypes of Bioreactors / Fermenters
Types of Bioreactors / Fermenters
ajithnandanam
 
Down Stream Processing
Down Stream ProcessingDown Stream Processing
Down Stream Processing
Chanakya P
 
FERMENTATION TYPES .
FERMENTATION TYPES .FERMENTATION TYPES .
FERMENTATION TYPES .
Shylesh M
 
Airlift fermenter
Airlift fermenterAirlift fermenter
Airlift fermenter
parnavi kadam
 
Upstream Processing
Upstream ProcessingUpstream Processing
Upstream Processing
AliHusnainWaince1
 
Industrial Production of L-Lysine by Fermentation
Industrial Production of L-Lysine by FermentationIndustrial Production of L-Lysine by Fermentation
Industrial Production of L-Lysine by Fermentation
Kuldeep Sharma
 
47805741 crystallization-whole-broth-processing
47805741 crystallization-whole-broth-processing47805741 crystallization-whole-broth-processing
47805741 crystallization-whole-broth-processing
78686
 
4.1
4.14.1
4.1
cheenul
 
Strain development techniques of industrially important microorganisms
Strain development techniques of industrially important microorganismsStrain development techniques of industrially important microorganisms
Strain development techniques of industrially important microorganisms
Microbiology
 
Inoculum development.pptx
Inoculum development.pptxInoculum development.pptx
Inoculum development.pptx
Vel Kumar
 

What's hot (20)

Fluidised Bed Bioreactor
Fluidised Bed BioreactorFluidised Bed Bioreactor
Fluidised Bed Bioreactor
 
Media for industrial fermentation
Media for industrial fermentationMedia for industrial fermentation
Media for industrial fermentation
 
Batch, fedbatch and continuous fermentation
Batch, fedbatch and continuous fermentationBatch, fedbatch and continuous fermentation
Batch, fedbatch and continuous fermentation
 
FERMENTERS( BIOREACTORS) AND THEIR TYPES
FERMENTERS( BIOREACTORS) AND THEIR TYPESFERMENTERS( BIOREACTORS) AND THEIR TYPES
FERMENTERS( BIOREACTORS) AND THEIR TYPES
 
Stirred tank bioreactor
Stirred tank bioreactorStirred tank bioreactor
Stirred tank bioreactor
 
Fermentor
Fermentor   Fermentor
Fermentor
 
strain improvement techniques
strain improvement techniquesstrain improvement techniques
strain improvement techniques
 
Media formulation
Media formulationMedia formulation
Media formulation
 
Aeration and agitation
Aeration and agitationAeration and agitation
Aeration and agitation
 
Downstream processing - industrial microbiology
Downstream processing - industrial microbiology Downstream processing - industrial microbiology
Downstream processing - industrial microbiology
 
Types of Bioreactors / Fermenters
Types of Bioreactors / FermentersTypes of Bioreactors / Fermenters
Types of Bioreactors / Fermenters
 
Down Stream Processing
Down Stream ProcessingDown Stream Processing
Down Stream Processing
 
FERMENTATION TYPES .
FERMENTATION TYPES .FERMENTATION TYPES .
FERMENTATION TYPES .
 
Airlift fermenter
Airlift fermenterAirlift fermenter
Airlift fermenter
 
Upstream Processing
Upstream ProcessingUpstream Processing
Upstream Processing
 
Industrial Production of L-Lysine by Fermentation
Industrial Production of L-Lysine by FermentationIndustrial Production of L-Lysine by Fermentation
Industrial Production of L-Lysine by Fermentation
 
47805741 crystallization-whole-broth-processing
47805741 crystallization-whole-broth-processing47805741 crystallization-whole-broth-processing
47805741 crystallization-whole-broth-processing
 
4.1
4.14.1
4.1
 
Strain development techniques of industrially important microorganisms
Strain development techniques of industrially important microorganismsStrain development techniques of industrially important microorganisms
Strain development techniques of industrially important microorganisms
 
Inoculum development.pptx
Inoculum development.pptxInoculum development.pptx
Inoculum development.pptx
 

Similar to Batch and Continuous Sterilization of Media in Fermentation Industry

Batch and Continuous Sterilization techniques
Batch and Continuous Sterilization techniquesBatch and Continuous Sterilization techniques
Batch and Continuous Sterilization techniques
SasiK25
 
Batch and continuous sterilization techniques
Batch and continuous sterilization techniquesBatch and continuous sterilization techniques
Batch and continuous sterilization techniques
SasiK25
 
3.STERILIZATION..-1.ppt
3.STERILIZATION..-1.ppt3.STERILIZATION..-1.ppt
3.STERILIZATION..-1.ppt
AbwoneKenneth
 
FEW_Exp 12.pdf
FEW_Exp 12.pdfFEW_Exp 12.pdf
FEW_Exp 12.pdf
HarshaSarma
 
Drying
DryingDrying
drying-171229080826.pdf
drying-171229080826.pdfdrying-171229080826.pdf
drying-171229080826.pdf
SabihQamat
 
Newer methods of thermal processing
Newer methods of thermal processing Newer methods of thermal processing
Newer methods of thermal processing
Kamaraj College of Engineering & Technology, Virudhunagar
 
Greenhouse technology
Greenhouse technologyGreenhouse technology
Greenhouse technology
Md Irfan Ansari
 
Heat Recovery System in Domestic Refrigerator
Heat Recovery System in Domestic RefrigeratorHeat Recovery System in Domestic Refrigerator
Heat Recovery System in Domestic Refrigerator
Ijrdt Journal
 
Newer methods of thermal processing - Dr KG/KCET
Newer methods of thermal processing - Dr KG/KCETNewer methods of thermal processing - Dr KG/KCET
Newer methods of thermal processing - Dr KG/KCET
Kamaraj College of Engineering & Technology, Virudhunagar
 
Evaporation: fundamentals and its design
Evaporation: fundamentals and its designEvaporation: fundamentals and its design
Evaporation: fundamentals and its design
Kulwinder Verma
 
13 sterillisation
13 sterillisation13 sterillisation
13 sterillisation
Gaju Shete
 
Fbd(Fluidized Bed Dryer)
Fbd(Fluidized Bed Dryer)Fbd(Fluidized Bed Dryer)
Fbd(Fluidized Bed Dryer)
Nandhini Podamekala
 
Air preheater hot air generator manufacturer
Air preheater   hot air generator manufacturerAir preheater   hot air generator manufacturer
Air preheater hot air generator manufacturer
Preeti Agarwal
 
Sterilization
SterilizationSterilization
Sterilization
Dr.Ahmed Emad
 
thermal processing of food
thermal processing of food thermal processing of food
thermal processing of food
Dr. Ganesh Shelke
 
fsm 9 presentation.pdf
fsm 9 presentation.pdffsm 9 presentation.pdf
fsm 9 presentation.pdf
JellymarieGasulasAnt
 
Humidication in NICU - Dr Padmesh - Neonatology
Humidication in NICU - Dr Padmesh - NeonatologyHumidication in NICU - Dr Padmesh - Neonatology
Humidication in NICU - Dr Padmesh - Neonatology
Dr Padmesh Vadakepat
 
Asphalt Testing For Precision, Accuracy And Maximum Throughput
Asphalt Testing For Precision, Accuracy And Maximum ThroughputAsphalt Testing For Precision, Accuracy And Maximum Throughput
Asphalt Testing For Precision, Accuracy And Maximum Throughput
John_Casola
 
instrument in microbiology.pptx
instrument in microbiology.pptxinstrument in microbiology.pptx
instrument in microbiology.pptx
kareem
 

Similar to Batch and Continuous Sterilization of Media in Fermentation Industry (20)

Batch and Continuous Sterilization techniques
Batch and Continuous Sterilization techniquesBatch and Continuous Sterilization techniques
Batch and Continuous Sterilization techniques
 
Batch and continuous sterilization techniques
Batch and continuous sterilization techniquesBatch and continuous sterilization techniques
Batch and continuous sterilization techniques
 
3.STERILIZATION..-1.ppt
3.STERILIZATION..-1.ppt3.STERILIZATION..-1.ppt
3.STERILIZATION..-1.ppt
 
FEW_Exp 12.pdf
FEW_Exp 12.pdfFEW_Exp 12.pdf
FEW_Exp 12.pdf
 
Drying
DryingDrying
Drying
 
drying-171229080826.pdf
drying-171229080826.pdfdrying-171229080826.pdf
drying-171229080826.pdf
 
Newer methods of thermal processing
Newer methods of thermal processing Newer methods of thermal processing
Newer methods of thermal processing
 
Greenhouse technology
Greenhouse technologyGreenhouse technology
Greenhouse technology
 
Heat Recovery System in Domestic Refrigerator
Heat Recovery System in Domestic RefrigeratorHeat Recovery System in Domestic Refrigerator
Heat Recovery System in Domestic Refrigerator
 
Newer methods of thermal processing - Dr KG/KCET
Newer methods of thermal processing - Dr KG/KCETNewer methods of thermal processing - Dr KG/KCET
Newer methods of thermal processing - Dr KG/KCET
 
Evaporation: fundamentals and its design
Evaporation: fundamentals and its designEvaporation: fundamentals and its design
Evaporation: fundamentals and its design
 
13 sterillisation
13 sterillisation13 sterillisation
13 sterillisation
 
Fbd(Fluidized Bed Dryer)
Fbd(Fluidized Bed Dryer)Fbd(Fluidized Bed Dryer)
Fbd(Fluidized Bed Dryer)
 
Air preheater hot air generator manufacturer
Air preheater   hot air generator manufacturerAir preheater   hot air generator manufacturer
Air preheater hot air generator manufacturer
 
Sterilization
SterilizationSterilization
Sterilization
 
thermal processing of food
thermal processing of food thermal processing of food
thermal processing of food
 
fsm 9 presentation.pdf
fsm 9 presentation.pdffsm 9 presentation.pdf
fsm 9 presentation.pdf
 
Humidication in NICU - Dr Padmesh - Neonatology
Humidication in NICU - Dr Padmesh - NeonatologyHumidication in NICU - Dr Padmesh - Neonatology
Humidication in NICU - Dr Padmesh - Neonatology
 
Asphalt Testing For Precision, Accuracy And Maximum Throughput
Asphalt Testing For Precision, Accuracy And Maximum ThroughputAsphalt Testing For Precision, Accuracy And Maximum Throughput
Asphalt Testing For Precision, Accuracy And Maximum Throughput
 
instrument in microbiology.pptx
instrument in microbiology.pptxinstrument in microbiology.pptx
instrument in microbiology.pptx
 

More from Dr. Pavan Kundur

Auto Immune Diosorder are conditions in which your immune system mistakenly d...
Auto Immune Diosorder are conditions in which your immune system mistakenly d...Auto Immune Diosorder are conditions in which your immune system mistakenly d...
Auto Immune Diosorder are conditions in which your immune system mistakenly d...
Dr. Pavan Kundur
 
Biology For Engineers Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2
Biology For Engineers  Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2 Biology For Engineers  Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2
Biology For Engineers Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2
Dr. Pavan Kundur
 
Biology for Engineers Module - 5 Trends in Bioengineering
Biology for Engineers Module - 5 Trends in BioengineeringBiology for Engineers Module - 5 Trends in Bioengineering
Biology for Engineers Module - 5 Trends in Bioengineering
Dr. Pavan Kundur
 
Biology for engineers Module 4 pdf.pdf
Biology for engineers Module 4 pdf.pdfBiology for engineers Module 4 pdf.pdf
Biology for engineers Module 4 pdf.pdf
Dr. Pavan Kundur
 
Module 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNS
Module 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNSModule 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNS
Module 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNS
Dr. Pavan Kundur
 
DNA Preparation for sequencing
DNA Preparation for sequencingDNA Preparation for sequencing
DNA Preparation for sequencing
Dr. Pavan Kundur
 
Introduction to Health
Introduction to HealthIntroduction to Health
Introduction to Health
Dr. Pavan Kundur
 
Hypersensitivity
Hypersensitivity Hypersensitivity
Hypersensitivity
Dr. Pavan Kundur
 
Artificial Intelligence in Agriculture
Artificial Intelligence in AgricultureArtificial Intelligence in Agriculture
Artificial Intelligence in Agriculture
Dr. Pavan Kundur
 
Preparing Genomic DNA for Sequencing
Preparing Genomic DNA for SequencingPreparing Genomic DNA for Sequencing
Preparing Genomic DNA for Sequencing
Dr. Pavan Kundur
 
Plant tissue culture
Plant tissue culturePlant tissue culture
Plant tissue culture
Dr. Pavan Kundur
 
Terminator Gene Technology
Terminator Gene TechnologyTerminator Gene Technology
Terminator Gene Technology
Dr. Pavan Kundur
 
Golden Rice
Golden Rice Golden Rice
Golden Rice
Dr. Pavan Kundur
 
Bt Cotton Development Stages
Bt Cotton Development StagesBt Cotton Development Stages
Bt Cotton Development Stages
Dr. Pavan Kundur
 
Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...
Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...
Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...
Dr. Pavan Kundur
 
Plant Growth Regulators
Plant Growth RegulatorsPlant Growth Regulators
Plant Growth Regulators
Dr. Pavan Kundur
 
What Next After B.Sc., Biotechnology and Microbiology Avenues / Careers
What Next After B.Sc., Biotechnology and Microbiology Avenues / CareersWhat Next After B.Sc., Biotechnology and Microbiology Avenues / Careers
What Next After B.Sc., Biotechnology and Microbiology Avenues / Careers
Dr. Pavan Kundur
 
Hybridization Techniques in Crop Improvement
Hybridization Techniques in Crop ImprovementHybridization Techniques in Crop Improvement
Hybridization Techniques in Crop Improvement
Dr. Pavan Kundur
 
Crop improvement / Space breeding
Crop improvement / Space breeding Crop improvement / Space breeding
Crop improvement / Space breeding
Dr. Pavan Kundur
 
Scope of Industrial Microbiology and Biotechnology
Scope of Industrial Microbiology and BiotechnologyScope of Industrial Microbiology and Biotechnology
Scope of Industrial Microbiology and Biotechnology
Dr. Pavan Kundur
 

More from Dr. Pavan Kundur (20)

Auto Immune Diosorder are conditions in which your immune system mistakenly d...
Auto Immune Diosorder are conditions in which your immune system mistakenly d...Auto Immune Diosorder are conditions in which your immune system mistakenly d...
Auto Immune Diosorder are conditions in which your immune system mistakenly d...
 
Biology For Engineers Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2
Biology For Engineers  Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2 Biology For Engineers  Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2
Biology For Engineers Module 3 / HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2
 
Biology for Engineers Module - 5 Trends in Bioengineering
Biology for Engineers Module - 5 Trends in BioengineeringBiology for Engineers Module - 5 Trends in Bioengineering
Biology for Engineers Module - 5 Trends in Bioengineering
 
Biology for engineers Module 4 pdf.pdf
Biology for engineers Module 4 pdf.pdfBiology for engineers Module 4 pdf.pdf
Biology for engineers Module 4 pdf.pdf
 
Module 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNS
Module 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNSModule 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNS
Module 2:- HUMAN ORGAN SYSTEMS AND BIO DESIGNS
 
DNA Preparation for sequencing
DNA Preparation for sequencingDNA Preparation for sequencing
DNA Preparation for sequencing
 
Introduction to Health
Introduction to HealthIntroduction to Health
Introduction to Health
 
Hypersensitivity
Hypersensitivity Hypersensitivity
Hypersensitivity
 
Artificial Intelligence in Agriculture
Artificial Intelligence in AgricultureArtificial Intelligence in Agriculture
Artificial Intelligence in Agriculture
 
Preparing Genomic DNA for Sequencing
Preparing Genomic DNA for SequencingPreparing Genomic DNA for Sequencing
Preparing Genomic DNA for Sequencing
 
Plant tissue culture
Plant tissue culturePlant tissue culture
Plant tissue culture
 
Terminator Gene Technology
Terminator Gene TechnologyTerminator Gene Technology
Terminator Gene Technology
 
Golden Rice
Golden Rice Golden Rice
Golden Rice
 
Bt Cotton Development Stages
Bt Cotton Development StagesBt Cotton Development Stages
Bt Cotton Development Stages
 
Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...
Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...
Anti-foaming agents, inducers, precursors and inhibitors in Fermentation tech...
 
Plant Growth Regulators
Plant Growth RegulatorsPlant Growth Regulators
Plant Growth Regulators
 
What Next After B.Sc., Biotechnology and Microbiology Avenues / Careers
What Next After B.Sc., Biotechnology and Microbiology Avenues / CareersWhat Next After B.Sc., Biotechnology and Microbiology Avenues / Careers
What Next After B.Sc., Biotechnology and Microbiology Avenues / Careers
 
Hybridization Techniques in Crop Improvement
Hybridization Techniques in Crop ImprovementHybridization Techniques in Crop Improvement
Hybridization Techniques in Crop Improvement
 
Crop improvement / Space breeding
Crop improvement / Space breeding Crop improvement / Space breeding
Crop improvement / Space breeding
 
Scope of Industrial Microbiology and Biotechnology
Scope of Industrial Microbiology and BiotechnologyScope of Industrial Microbiology and Biotechnology
Scope of Industrial Microbiology and Biotechnology
 

Recently uploaded

Contiguity Of Various Message Forms - Rupam Chandra.pptx
Contiguity Of Various Message Forms - Rupam Chandra.pptxContiguity Of Various Message Forms - Rupam Chandra.pptx
Contiguity Of Various Message Forms - Rupam Chandra.pptx
Kalna College
 
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapitolTechU
 
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdfمصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
سمير بسيوني
 
How to Download & Install Module From the Odoo App Store in Odoo 17
How to Download & Install Module From the Odoo App Store in Odoo 17How to Download & Install Module From the Odoo App Store in Odoo 17
How to Download & Install Module From the Odoo App Store in Odoo 17
Celine George
 
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...
TechSoup
 
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...
indexPub
 
The basics of sentences session 7pptx.pptx
The basics of sentences session 7pptx.pptxThe basics of sentences session 7pptx.pptx
The basics of sentences session 7pptx.pptx
heathfieldcps1
 
Bossa N’ Roll Records by Ismael Vazquez.
Bossa N’ Roll Records by Ismael Vazquez.Bossa N’ Roll Records by Ismael Vazquez.
Bossa N’ Roll Records by Ismael Vazquez.
IsmaelVazquez38
 
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...
Nguyen Thanh Tu Collection
 
HYPERTENSION - SLIDE SHARE PRESENTATION.
HYPERTENSION - SLIDE SHARE PRESENTATION.HYPERTENSION - SLIDE SHARE PRESENTATION.
HYPERTENSION - SLIDE SHARE PRESENTATION.
deepaannamalai16
 
Simple-Present-Tense xxxxxxxxxxxxxxxxxxx
Simple-Present-Tense xxxxxxxxxxxxxxxxxxxSimple-Present-Tense xxxxxxxxxxxxxxxxxxx
Simple-Present-Tense xxxxxxxxxxxxxxxxxxx
RandolphRadicy
 
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
220711130083 SUBHASHREE RAKSHIT  Internet resources for social science220711130083 SUBHASHREE RAKSHIT  Internet resources for social science
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
Kalna College
 
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
Nguyen Thanh Tu Collection
 
Skimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S EliotSkimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S Eliot
nitinpv4ai
 
Observational Learning
Observational Learning Observational Learning
Observational Learning
sanamushtaq922
 
Pharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brubPharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brub
danielkiash986
 
Wound healing PPT
Wound healing PPTWound healing PPT
Wound healing PPT
Jyoti Chand
 
Oliver Asks for More by Charles Dickens (9)
Oliver Asks for More by Charles Dickens (9)Oliver Asks for More by Charles Dickens (9)
Oliver Asks for More by Charles Dickens (9)
nitinpv4ai
 
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
Payaamvohra1
 
Juneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School DistrictJuneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School District
David Douglas School District
 

Recently uploaded (20)

Contiguity Of Various Message Forms - Rupam Chandra.pptx
Contiguity Of Various Message Forms - Rupam Chandra.pptxContiguity Of Various Message Forms - Rupam Chandra.pptx
Contiguity Of Various Message Forms - Rupam Chandra.pptx
 
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
 
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdfمصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
 
How to Download & Install Module From the Odoo App Store in Odoo 17
How to Download & Install Module From the Odoo App Store in Odoo 17How to Download & Install Module From the Odoo App Store in Odoo 17
How to Download & Install Module From the Odoo App Store in Odoo 17
 
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...
 
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...
 
The basics of sentences session 7pptx.pptx
The basics of sentences session 7pptx.pptxThe basics of sentences session 7pptx.pptx
The basics of sentences session 7pptx.pptx
 
Bossa N’ Roll Records by Ismael Vazquez.
Bossa N’ Roll Records by Ismael Vazquez.Bossa N’ Roll Records by Ismael Vazquez.
Bossa N’ Roll Records by Ismael Vazquez.
 
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 8 - CẢ NĂM - FRIENDS PLUS - NĂM HỌC 2023-2024 (B...
 
HYPERTENSION - SLIDE SHARE PRESENTATION.
HYPERTENSION - SLIDE SHARE PRESENTATION.HYPERTENSION - SLIDE SHARE PRESENTATION.
HYPERTENSION - SLIDE SHARE PRESENTATION.
 
Simple-Present-Tense xxxxxxxxxxxxxxxxxxx
Simple-Present-Tense xxxxxxxxxxxxxxxxxxxSimple-Present-Tense xxxxxxxxxxxxxxxxxxx
Simple-Present-Tense xxxxxxxxxxxxxxxxxxx
 
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
220711130083 SUBHASHREE RAKSHIT  Internet resources for social science220711130083 SUBHASHREE RAKSHIT  Internet resources for social science
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
 
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
 
Skimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S EliotSkimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S Eliot
 
Observational Learning
Observational Learning Observational Learning
Observational Learning
 
Pharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brubPharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brub
 
Wound healing PPT
Wound healing PPTWound healing PPT
Wound healing PPT
 
Oliver Asks for More by Charles Dickens (9)
Oliver Asks for More by Charles Dickens (9)Oliver Asks for More by Charles Dickens (9)
Oliver Asks for More by Charles Dickens (9)
 
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
 
Juneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School DistrictJuneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School District
 

Batch and Continuous Sterilization of Media in Fermentation Industry

  • 1. Batch and Continuous Sterilization Sterilization of Media for Fermentation Dr. Pavan J Kundur Assistant Professor P C Jabin Science College Hubballi
  • 2.  Batch Sterilization is the reduction of contaminant organisms through the heating of a vessel. The entire volume of media is sterilized at once through the use of thermal or radiation techniques. When running a thermal batch sterilization, a system goes through 3 steps: heating, holding, and cooling. Heating requires the addition of energy throughout the entire medium volume. This can be done by adding heat through a jacket on the vessel.
  • 3. The temperature is increased until it reaches the sterilization temperature where it is held for a set period of time. During this phase, most of the unwanted microorganisms are destroyed. Finally, the system is cooled to bring the sterile media back to the desired temperature. For radiation sterilization, the process is similar to above, although it uses radiation intensity instead of heat.
  • 4.
  • 5. In order to sterilize a batch, calculate the total area underneath the curve. Therefore, model death using first order kinetics and integrate as seen above. This will yield a temperature and the corresponding duration of time needed to sterilize the media. Advantages: Most widely used technique Simple operation No additional materials are added to the media itself Disadvantages: More expensive heat requirements than continuous sterilization Best results occur in well-mixed closed vessels
  • 7.  Continuous sterilization is the rapid transfer of heat to medium through steam condensate without the use of a heat exchanger.  Once the media is in a holding loop, steam is injected to the system via a nozzle. The medium stays in this loop for a predetermined holding time until the entire medium is sterile.  This is more efficient than batch sterilization because instead of expending energy to heat, hold, and cool the entire system, small portions of the inlet streams are heated at a time.  By looping sterile media tubes (which are at higher temperatures) past inlet tubes, the difference in temperature is used to help heat the unsterile medium.  So instead of having a cold-water stream cool the sterile media, the lower temperature unsterile media stream absorbs heat from the warm stream, cooling the sterile media.  Finally, the sterile media is flash cooled through an expansion valve to adjust the temperature to meet process parameters.
  • 8.
  • 9. Advantages: Uniform steam requirements throughout the duration of the sterilization Simplified process control Shorter sterilization time means less thermal degradation of medium Disadvantages: High demand for steam in a shorter period of time than batch Concentration of media becomes dilute due to steam condensation Since steam is actually dispersed in media, steam must be clean to avoid contamination
  • 10. Advantages of Continuous Sterilization process over Batch Sterilization
  • 11.  Better protection of medium value .  Batch sterilization wastes energy and can overcook the medium  Batch sterilization uses steam or direct heat to elevate the temperature. Both the heat and the cooling water are spent with no opportunity for energy recovery.  temperature in batch sterilization can not increased beyond 121°c as if the temperature is increased, the heating & cooling period increases .  hence during sterilization the nutrient media will be degraded .
  • 12.  Continuous Sterilization is the method of choice .  In Continuous Sterilization , the medium to be sterilized is dealt in small increments &  hence there is shorter heating & cooling periods.  much higher temperature can be utilized , thus reducing holding time.  therefore, in continuous sterilization nutrient degradation is subsequently less as compared to batch sterilization .  This is more efficient than batch sterilization because instead of expending energy to heat, hold, and cool the entire system, small portions of the inlet streams are heated at a time
  • 13.  Most of the sterilization in the continuous sterilization process may occurs in the holding The holding section is a long pipe, λhold = L uav length of the pipe average fluid velocity in the pipe
  • 14.  High steam utilization capacity. As compared to batch sterilization  The heat can be fully utilized during sterilizing process and  therefore, great amount of water and steam can be saved as well as energy consumption is decreased substantially.  It can averagely save about 30% of steam and 40% of cooling water throughout the sterilization process if Continuous Sterilization Technology is applied.
  • 15.  Easy Scaling-up of the process .  On scaling up the fermentation it was very difficult to attain the correct sterilization conditions using the batch regime .  Continuous sterilization using direct steam injection allowed the design of a precise process producing sterile medium with the required degree of interaction b/w the ingredients . The temperature profile of the medium allowing an easy estimate of the Del factor (∆) required. This makes scale-up very simple.
  • 16.  Easier to automate the process  The Continuous Sterilizer is not a pressure vessel and is easy to automate the whole process .  hence improves the quality of sterilization & reduce labor intensive  Reduces manpower, machinery replacement and maintenance  Reduction of sterilization cycle time