The document discusses antiseptics and disinfectants. It defines key terms like asepsis, fungicide, sporicides, and provides examples of common antiseptics and disinfectants used. These include phenol, iodine compounds, boric acid, acids, alkalies, alcohols, hydrogen peroxide, and potassium permanganate. The modes of action and ideal properties of antiseptics and disinfectants are described. Factors affecting their efficacy like nature of microbes, concentration, and temperature are also outlined.
Because microbial cytoplasm is usually transparent, it is necessary to stain microorganisms before they can be viewed with the light microscope. In some cases, staining is unnecessary, for example when microorganisms are very large or when motility is to be studied, and a drop of the microorganisms can be placed directly on the slide and observed.
This ppt includes all the key points of process of sterilization and its different techniques like physical,chemical,thermal,etc. sterilization is very important topic to go through during education as well as during practice to maintain a nice infection free environment of your health care office or clinic.
Classification and mode of action of disinfectants PHARMACEUTICAL MICROBIOLOG...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-III Classification and mode of action of disinfectants. DISINFECTANT
Definition: Ideal properties of disinfectants: CLASSIFICATION OF DISINFECTANTS: Based on consistency 1. Liquid (E.g., Alcohols, Phenols) 2.Gaseous (Formaldehyde vapor, Ethylene oxide). Based on spectrum of activity 1. High level disinfectant
2. Intermediate level disinfectant
3. Low level disinfectant .Based on mechanism of action: 1.Action on membrane2.Denaturation of cellular proteins 3.Damage to nucleic acids 4.Oxidation of essential sulfhydryl groups of enzymes 5.Alkylation of amino-, carboxyl- and hydroxyl group. MODE OF ACTION AND APPICATION OF DISINFECTANT
Acid and alkalies
Halogens
Heavy metals
Phenols and its derivatives
Alcohol
Aldehydes
Dyes:
Quaternary ammonium compounds
Detergents and soaps.
Environmental health is the branch of public health concerned with all aspects of the natural and built environment affecting human health. In order to effectively control factors that may affect health, the requirements that must be met in order to create a healthy environment must be determined.[1] The major sub-disciplines of environmental health are environmental science, toxicology, environmental epidemiology, and environmental and occupational medicine.[2]
Definitions
WHO definitions
Environmental health was defined in a 1989 document by the World Health Organization (WHO) as: Those aspects of human health and disease that are determined by factors in the environment.[citation needed] It is also referred to as the theory and practice of accessing and controlling factors in the environment that can potentially affect health.[citation needed]
A 1990 WHO document states that environmental health, as used by the WHO Regional Office for Europe, "includes both the direct pathological effects of chemicals, radiation and some biological agents, and the effects (often indirect) on health and well being of the broad physical, psychological, social and cultural environment, which includes housing, urban development, land use and transport."[3]
As of 2016, the WHO website on environmental health states that "Environmental health addresses all the physical, chemical, and biological factors external to a person, and all the related factors impacting behaviours. It encompasses the assessment and control of those environmental factors that can potentially affect health. It is targeted towards preventing disease and creating health-supportive environments. This definition excludes behaviour not related to environment, as well as behaviour related to the social and cultural environment, as well as genetics."[4]
The WHO has also defined environmental health services as "those services which implement environmental health policies through monitoring and control activities. They also carry out that role by promoting the improvement of environmental parameters and by encouraging the use of environmentally friendly and healthy technologies and behaviors. They also have a leading role in developing and suggesting new policy areas."[5][6]
Other considerations
The term environmental medicine may be seen as a medical specialty, or branch of the broader field of environmental health.[7][8] Terminology is not fully established, and in many European countries they are used interchangeably.[9]
Children's environmental health is the academic discipline that studies how environmental exposures in early life—chemical, nutritional, and social—influence health and development in childhood and across the entire human life span.[10]
Other terms referring to or concerning environmental health include environmental public health and health protection.
Disciplines
Five basic disciplines generally contribute to the field of environmental health: environmental epidemiology,
Because microbial cytoplasm is usually transparent, it is necessary to stain microorganisms before they can be viewed with the light microscope. In some cases, staining is unnecessary, for example when microorganisms are very large or when motility is to be studied, and a drop of the microorganisms can be placed directly on the slide and observed.
This ppt includes all the key points of process of sterilization and its different techniques like physical,chemical,thermal,etc. sterilization is very important topic to go through during education as well as during practice to maintain a nice infection free environment of your health care office or clinic.
Classification and mode of action of disinfectants PHARMACEUTICAL MICROBIOLOG...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-III Classification and mode of action of disinfectants. DISINFECTANT
Definition: Ideal properties of disinfectants: CLASSIFICATION OF DISINFECTANTS: Based on consistency 1. Liquid (E.g., Alcohols, Phenols) 2.Gaseous (Formaldehyde vapor, Ethylene oxide). Based on spectrum of activity 1. High level disinfectant
2. Intermediate level disinfectant
3. Low level disinfectant .Based on mechanism of action: 1.Action on membrane2.Denaturation of cellular proteins 3.Damage to nucleic acids 4.Oxidation of essential sulfhydryl groups of enzymes 5.Alkylation of amino-, carboxyl- and hydroxyl group. MODE OF ACTION AND APPICATION OF DISINFECTANT
Acid and alkalies
Halogens
Heavy metals
Phenols and its derivatives
Alcohol
Aldehydes
Dyes:
Quaternary ammonium compounds
Detergents and soaps.
Environmental health is the branch of public health concerned with all aspects of the natural and built environment affecting human health. In order to effectively control factors that may affect health, the requirements that must be met in order to create a healthy environment must be determined.[1] The major sub-disciplines of environmental health are environmental science, toxicology, environmental epidemiology, and environmental and occupational medicine.[2]
Definitions
WHO definitions
Environmental health was defined in a 1989 document by the World Health Organization (WHO) as: Those aspects of human health and disease that are determined by factors in the environment.[citation needed] It is also referred to as the theory and practice of accessing and controlling factors in the environment that can potentially affect health.[citation needed]
A 1990 WHO document states that environmental health, as used by the WHO Regional Office for Europe, "includes both the direct pathological effects of chemicals, radiation and some biological agents, and the effects (often indirect) on health and well being of the broad physical, psychological, social and cultural environment, which includes housing, urban development, land use and transport."[3]
As of 2016, the WHO website on environmental health states that "Environmental health addresses all the physical, chemical, and biological factors external to a person, and all the related factors impacting behaviours. It encompasses the assessment and control of those environmental factors that can potentially affect health. It is targeted towards preventing disease and creating health-supportive environments. This definition excludes behaviour not related to environment, as well as behaviour related to the social and cultural environment, as well as genetics."[4]
The WHO has also defined environmental health services as "those services which implement environmental health policies through monitoring and control activities. They also carry out that role by promoting the improvement of environmental parameters and by encouraging the use of environmentally friendly and healthy technologies and behaviors. They also have a leading role in developing and suggesting new policy areas."[5][6]
Other considerations
The term environmental medicine may be seen as a medical specialty, or branch of the broader field of environmental health.[7][8] Terminology is not fully established, and in many European countries they are used interchangeably.[9]
Children's environmental health is the academic discipline that studies how environmental exposures in early life—chemical, nutritional, and social—influence health and development in childhood and across the entire human life span.[10]
Other terms referring to or concerning environmental health include environmental public health and health protection.
Disciplines
Five basic disciplines generally contribute to the field of environmental health: environmental epidemiology,
A Presentation on Biopesticides, its types, uses, applications and mechanism of action.
CONTENTS:
Definition of Biopesticides
Introduction
Uses of Biopesticides
Types of Biopesticides
Mechanism of Biopesticides
Merits of Biopesticides over Chemical Pesticides
Limitations of Chemical Insecticides
Reasons for the limited use of Biopesticides
Commercial Exploitation of Biopesticides
Conclusion
References
STERILIZATION AND DISINFECTION BY DR RAHUL ACHARYA.pptrahulacharya52
sterilization using chemical methods, sterilization using physical methods, sterilization using sun light, heat, autoclave, hot air oven . tindalisation, inspisation .
Control of microrganisms - Food Microbiology - Food Processing MUTHUGANESAN N
1. Sterilization - the destruction of all microorganisms, including endospores, on an object or in a material.
2. Disinfection - the destruction of pathogens, but not endospores, on an object or in a material. The number of pathogens is reduced or growth is inhibited to a level that does not produce disease.
3. Antisepsis - chemical disinfection of the skin, mucosal membranes, or other living tissues.
4. Germicide ("cide" = kill) - a chemical agent that rapidly kills microorganisms.
Specific germicides include:
(I) Sporicide - kills spores
(II) Bactericide - kills bacteria
(III) Viricide - kills viruses
(IV) Fungicide - kills fungi
At the end of this session learner will be able to:
Define Common terms.
Explain the importance of microorganisms control.
Discuss the Methods of sterilization.
Categorize the broad spectrum and narrow spectrum antibiotics.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
2. Friday, March 27, 2020 AMIR SOHAIL
Definitions:
Asepsis:
Sterilization of instruments, dressing materials
etc. through use of heat or radiation
Fungicide:
A substance that destroys fungi
Sporicides:
Destroy spores
3. Friday, March 27, 2020 dr. ovais
Sanitizer:
Reduces number of bacterial contaminants
to a safe level
Preservatives:
Prevent decomposition by either chemical
or physical means
Deodorants:
To remove foul odors
4. Cleansers - to remove foreign material
Antiseptics - applied to living tissue to kill
microbes or to inhibit their growth
Disinfectants - to kill or inhibit
microorganisms on inanimate objects
Friday, March 27, 2020 dr. ovais
5. Friday, March 27, 2020 dr. ovais
Antiseptics!
Applied to living tissues for killing bacteria
or to inhibit their growth
Static or cidal effect on organisms
Safe limits for public health sanitization
Complete destruction of microbes
sterilization
6. Friday, March 27, 2020 dr. ovais
Disinfectants!
Germicidals applied to nonliving materials
Are very toxic for parenteral use
Collectively, both antiseptics &
disinfectants termed as germicides or local
anti-infective agents
7. Friday, March 27, 2020 dr. ovais
Some agents in lower concentrations act as
antiseptics but in higher concentrations are
disinfectants
8. Friday, March 27, 2020 dr. ovais
USES!
Topical anti-infectives are used in surgery
for antisepsis of surgical area and surgeons
hands and to disinfect surgical instruments,
operation room & hospital premises.
9. Friday, March 27, 2020 dr. ovais
Disinfectants as for homes & farm
premises, in water treatment, in public
health sanitation & as antiseptics in soaps,
teat dips & dairy sanitizers. Also been used
for treating local infections
Used for microbial control in both
prevention & treatment of diseases.
10. Friday, March 27, 2020 dr. ovais
NOTTTTTTTTTTT to be used as
Most of these locally acting anti-infective
drugs are unsuitable for systemic use
Low margins of safety
Toxic for internal organs
11. Friday, March 27, 2020 dr. ovais
Ideal Properties!
Chemically stable
Rapid in action
Broad spectrum (bact., viruses, fungi)
Potent germicidal activity
Good antimicrobial efficacy
12. Friday, March 27, 2020 dr. ovais
Effective in pus & necrotic tissues etc.
High lipid solubility
High penetrating properties
Long lasting effect
Non-irritant
13. Friday, March 27, 2020 dr. ovais
Should not be toxic to host
Should not impair wound healing
Offensive odor, color & staining properties
should be absent or minimal
Should not corrode or rust instruments
Economic & easily available
14. Friday, March 27, 2020 dr. ovais
Order of killing
Vegetative bacteria
Lipophilic viruses (lipid containing, enveloped)
Fungi
Hydrophilic viruses (non-lipid, non-enveloped)
Mycobacteria (Mycobacterium tuberculosis)
Bacterial spores
15. Friday, March 27, 2020 dr. ovais
Factors affecting antisep. & disinf.
Nature of microbes
Size of infection
Presence of organic matter
Concentration
Temperature & pH
16. Nature of Microbes:
Efficacy of germicide is influenced by types
of microorganisms.
Vegetative organisms are more susceptible
than the spore forming
G+ve are more susceptible than G-ve
organisms
Friday, March 27, 2020 dr. ovais
17. Size of infection:
In general, higher the level of microbial
contamination, lower is the efficacy of
germicides.
So long period of germicide exposure is
required.
Friday, March 27, 2020 dr. ovais
18. Presence of organic matter:
Presence of pus, blood, and tissue debris at
site of action significantly affects the
germicidal efficacy of many agents.
Faecal and soil contamination makes sheds
difficult to disinfect.
Friday, March 27, 2020 dr. ovais
19. Concentration:
Germicidal action is inversely dependent on
its concentration.
Increase or decrease in concentrations of
some compounds negatively affects the
efficacy.
Friday, March 27, 2020 dr. ovais
20. Temperature and pH:
Certain compounds are more active at
alkaline pH where as some are active at
acidic pH.
High ambient temperature usually results in
increased antimicrobial activity.
Friday, March 27, 2020 dr. ovais
21. Spectrum of Activity:
In general, antiseptics are less toxic to
microbes than the disinfectants
For disinfectants, there is a general scale of
innate resistance of microorganisms to
germicides.
From least resistance to most resistance, the scale is
vegetative bacteria, vegetative fungi and fungal spores,
enveloped viruses, non- enveloped viruses, protozoal
spore, mycobacteria and bacterial spores.
dr. ovaisFriday, March 27, 2020
22. Classes of Disinfectants:
Low-level disinfectants
Intermediate-level disinfectants
High-level disinfectants
Friday, March 27, 2020 dr. ovais
23. Low-level disinfectants
Rapidly kill vegetative forms of bacteria
and fungi
Used on animal cages, floors and food
bowls
Friday, March 27, 2020 dr. ovais
25. High-level disinfectants
Agents destroy all microorganisms and have
effectiveness against bacterial spores.
Used on needles, scalpels and scissors
Friday, March 27, 2020 dr. ovais
26. Friday, March 27, 2020 dr. ovais
Mode of Action!
By denaturation of intracellular protein
Alteration of cellular membrane
Enzyme inhibition
27. Friday, March 27, 2020 dr. ovais
Classification:
Chemical nature
Mode of action
28. Friday, March 27, 2020 dr. ovais
I-Physical Agents:
1. Heat------dry and moist
2. Light/Radiation------ultraviolet light
29. Friday, March 27, 2020 dr. ovais
II-Chemical Agents:
1. Acids:
a. Inorganic acid---boric acid, HCl,
Sulphuric acid
b. Organic acids---acetic acid, benzoic acid,
salicylic acid
2. Alkalis:---sodium hydroxide, calcium
oxide, calcium hydroxide
31. Friday, March 27, 2020 dr. ovais
6. Phenol & related compounds:---phenol,
cresol, lysol, hexachlorophene and
resorcinol
32. Friday, March 27, 2020 dr. ovais
7. Halogens & compounds:
a. Iodine & compounds:
i. Iodine---weak soln. of iodine, iodine
ointment, Mandl’s paint
ii. Iodophores:---povidone iodine
b. Chlorine & compounds:
i. Chlorine
ii. Chlorophores---sodium hypochlorite,
calcium hypochlorite, chlorinated lime
etc.
33. Friday, March 27, 2020 dr. ovais
8. Surface acting agents/surfactants
a. Anionic surfactants---soaps
b. Cationic surfactants---cetrimide,
benzalkonium chloride, benzethonium etc.
34. Friday, March 27, 2020 dr. ovais
9. Biguanides:
e.g chlorhexidine and hexamidine
10. Metallic compounds:
a. Silver compds.---silver nitrate
b. Zinc compds.---zinc sulphate, zinc oxide
c. Mercury compds.---mercuric chloride,
thiomersal
35. Friday, March 27, 2020 dr. ovais
11. Dyes:
a. Acridine dyes:---acriflavine
b. Rosaniline dye:---gentian violet, brilliant
green
c. Azo dyes:---scarlet red
37. I- Physical Agents:
1. Heat: Dry or moist heat is widely used
for sterilization and disinfection of
glassware, surgical and laboratory
equipments, dressing etc.
Heat destroys all type of microorganisms
including bacterial spores.
Friday, March 27, 2020 dr. ovais
38. Dry Heat
By using hot air oven
Microorganisms and bacterial spores are
killed.
To destroy all vegetative bacteria,
application of dry heat for 1.5 hrs at 100oC
is necessary
Mold spores are killed by 1.5 hrs at 115oC
Resistant spores by 3 hrs at 140oC
Friday, March 27, 2020 dr. ovais
39. Dry heat destroys microorganisms by
causing coagulation of proteins, oxidation
or incineration.
Dry heat is suitable for oily substances and
dry powders which are destroyed by moist
heat.
Less efficacious for surgical dressings
Friday, March 27, 2020 dr. ovais
40. Moist Heat:
Steam sterilization
Used widely for destroying microorganisms
By using hot water, boiling water, steam at
atmospheric pressure (steaming) or steam under
pressure (autoclaving) generally for 121oC for 15
minutes.
Both vegetative and spore forms of most bacteria
are killed by denaturation and co agulation of some
proteins and enzymes dr. ovaisFriday, March 27, 2020
41. Surgical dressings, glassware, rubber gloves
and surgical instruments are effectively
sterilized.
Moist heat has more penetrating power than
dry heat
Friday, March 27, 2020 dr. ovais
42. 2. Light/Radiations:
Ultra violet light: UV radiations are often
used for sterilization/disinfection of surgical
rooms and naked surfaces such as tabletops.
UV rays destroy vital structures of cells
such as nucleoproteins by which
microorganisms are killed.
G-ve non sporing organisms are most
susceptible.
Friday, March 27, 2020 dr. ovais
43. Friday, March 27, 2020 dr. ovais
Common Antiseptics &
Disinfectants:
Phenol, Iodine compounds, Boric Acid,
Acids, Alkalies, Lime, Soaps, Alcohols,
Mercurials, Hydrogen per oxide, Potassium
permangnate, Dyes, Acriflavine,
Formaldehyde, Heat, Dettol etc.
44. Friday, March 27, 2020 dr. ovais
Phenols (Carbolic Acid):
Caustic to tissues
Precipitate Proteins
At 1:90 it can kill many bacteria
Cause convulsions and renal toxicity
Cresol (Lysol), resorcinol and thymol are
some of the derivatives and are antisep. and
disinf.
Hexachlorophene, used in soaps and
creams.
45. Phenol has wide spectrum of activity
against bacteria, viruses and fungi but
minimal action on spores.
It acts by penetrating and disrupting cell
walls and denaturing bacterial proteins
It is bacteriostatic at conc. of 0.1 -1 % and
bactericidal/fungicidal at conc. of 1-2 %
Friday, March 27, 2020 dr. ovais
46. Friday, March 27, 2020 dr. ovais
Iodine Compounds:
2% tincture, as antisep. and disinf.
Stains skin
Irritant to tissues
Complex iodine compounds are less irritant like
povidone-iodine
A 1:20,000 solution kills most bacteria in 1
minute and spores in 15 minutes
Acts by iodinating and oxidising microbial
protoplasm
47. Iodine is present in solutions, ointments and
paints
0.25 % weak solution is used as uterine and
vaginal douche.
Mandl’s paint is applied over mm of sore
throat
Iodine ointment is widely used as antiseptic
and counter irritant
Friday, March 27, 2020 dr. ovais
48. Friday, March 27, 2020 dr. ovais
Boric Acid:
Weak bacteriostatic agent
Applied on delicate tissues
Non-irritating drying substance
Used as aqueous solution 4 %, lotions,
ointments or irrigating solutions.
Boroglycerine paint (30%) is used for
stomatitis and glossitis
Used in dusting powders, eye washes and
ear drops etc.
49. Friday, March 27, 2020 dr. ovais
Acids:
H2SO4 and HCl (0.1-1 N) as
disinfectant
Labs. and Hospitals
Very corrosive
Benzoic acid and salicylic acid
have germicidal and fungicidal
activities
50. Acetic acid:
0.25 % is useful for irrigation of urinary
tract
1 % for surgical dressings
1-3 % for burn dressings
At 5 %, bactericidal
99 % used as caustic on warts
Friday, March 27, 2020 dr. ovais
51. Benzoic acid:
Non-toxic
Applied on skin on high concentration
Bacteriostatic and antifungal agent used for
preservation of butter, meat, food or drinks.
Used in Whitfield’s ointment to treat
ringworm infection on skin
It also has keratolytic properties
Friday, March 27, 2020 dr. ovais
52. Several other organic acids like salicylic
acid, mandelic acid and trichloroacetic acid
(for warts) are used topically as antiseptic.
Friday, March 27, 2020 dr. ovais
53. Friday, March 27, 2020 dr. ovais
Alkalies:
Inhibit most of bacteria and viruses
94% soln. of NaOH to kill most pathogens
including FMDV and parasites
2% acts as disinf.
Liberate OH- ions which have antimicrobial
activity
Potent germicidal, used as disinfectant and caustic
Concentrated solution is used as caustic for
dishorning of calves
54. Sodium hydroxide
Potent germicide
Soln. is used as disinfectant (2%) and
caustic
Concentrated solution is used as caustic for
dehorning of calves
Friday, March 27, 2020 dr. ovais
55. Friday, March 27, 2020 dr. ovais
Soaps:
Gram+
Gram- resistant
Good with Potassium iodide and
Hexachlorophene
56. Friday, March 27, 2020 dr. ovais
Alcohols:
Ethyl Alcohol (Ethanol) and isopropyl
alcohol
More antisep. than disinf.
70% for instruments and syringes
These are highly flammable and may catch
fire during electrocautery or laser surgery.
57. Friday, March 27, 2020 dr. ovais
Hydrogen Peroxide: H2O2
Mechanically removes pus and cellular
debris from wound cavities
Low germicidal activity
Liberates free oxygen
A 3 % soln. is used for wound cleansing
58. Friday, March 27, 2020 dr. ovais
KMnO4
Strong Oxidizing agent, liberates Oxygen when
comes in contact with organic matter or bacteria
Strong antibacterial activity
Astringent, caustic, deodorant
1:3,000 is less irritant
1:1000 solutions are used for douching, irrigating
cavities and cleansing wounds
A 0.02% soln. is used as gastric lavage in poisoning
A 5% soln. is used as astringent
59. For fumigation, potassium permanganate is
used along with soln. of formaldehyde (1:2)
Used to disinfect water ponds and wells
Not good for surgical instruments due to
rusting property
Friday, March 27, 2020 dr. ovais
60. Friday, March 27, 2020 dr. ovais
Dyes:
Brilliant Green:
Surface antisep.
To treat wounds and burns
Crystal Violet:
Antisep., Skin infections due to fungi
0.5% soln. is used for local appliacation
61. Friday, March 27, 2020 dr. ovais
Acridine Dyes:
Acriflavine, euflavine and proflavine
Broad spectrum bacteriostatic and
bactericidals
Non irritant
Antiseptic on skin and mucous membrane
Cream, ointment and emulsion
More active against G+
62. Acriflavine
0.1 % as emulsion, aqueous or saline
solution, lotion, cream or jelly
Used for burns
Friday, March 27, 2020 dr. ovais
63. Friday, March 27, 2020 dr. ovais
Formaldehyde:
For preserving biological material
Externally it is strongly irritant and antiseptic
A 3-8% aq. Soln. is used as disinfectant
Fumes are irritant and does not penetrate well.
64. Friday, March 27, 2020 dr. ovais
Dettol: Chloroxylenol
Antisep. and disinfect.
Highly bactericidal and low toxicity
For skin sterilization, 1:4
For wound cleansing 1-2% soln.
Good for G+
Non corrosive and non irritant
65. Benzalkonium:
A biocidal agent
Active against bacteria, viruses, fungi and
protozoa
Bacteriostatic or bactericidal
G+ more susceptible
Used in eyewashes, nasal sprays, hand and
face washes, mouthwashes, cleaners,
sanitizers and disinfectants.
Friday, March 27, 2020 dr. ovais
66. Chlorhexidine:
Broad spectrum antibacterial activity
Limited fungicidal and virucidal properties
Used in shampoos, ointments, skin and
wound cleanser, mouthwashes, teat dips and
surgical scrubs.
Friday, March 27, 2020 dr. ovais
67. Silver nitrate:
A caustic, astringent and antibacterial
A 0.1% soln. is bactericidal
0.01% solution is bacteriostatic
Applied on burns to reduce infection
Used to destroy horn buds in calves
Friday, March 27, 2020 dr. ovais
68. Antibiotics:
Bacitracin, plymyxin B, neomycin,
framycetin and tyrothricin.
Sulphonamides like silver sulphadiazine
and mafenide are useful topical antibacterial
agents.
Nitrofurazone is highly efficacious in burns
and for skin grafting.
Friday, March 27, 2020 dr. ovais
69. Friday, March 27, 2020 dr. ovais
Microorganisms pathogenic
to humans