Sterilization is a process that eliminates all microorganisms, while disinfection only eliminates pathogenic or disease-causing ones. Physical sterilization methods include heat, radiation, filtration and drying. Moist heat using an autoclave is the most reliable sterilization method. Chemical agents like alcohol, aldehydes, halogens and phenols are also used for sterilization and disinfection. Proper cleaning is important before sterilization or disinfection. Sterilized items are classified based on the risk of infection if contaminated.
This document defines sterilization and disinfection, and discusses various physical and chemical agents used for these processes. It notes that sterilization aims to remove all microorganisms, while disinfection targets pathogenic organisms. Common physical agents for sterilization include heat, radiation, filtration and ultrasound. Chemical agents discussed include alcohols, aldehydes, dyes, halogens, phenols and gases. Proper sterilization requires consideration of multiple factors to effectively kill microbes.
This document provides definitions and outlines principles of sterilization and disinfection. It defines key terms like sterilization, disinfection, antiseptics, and antibiotics. It describes various physical agents (heat, radiation), chemical agents (gases, liquids), and mechanical removal methods used for microbial control. It discusses the mechanisms of action and structures of microbes targeted by sterilizers and disinfectants. It also provides details on specific processes like autoclaving, pasteurization, and chemical disinfectants like alcohols, aldehydes, halogens, and phenols.
Sterilization is a process that eliminates all microorganisms from an object or substance. It can be achieved through physical methods like heat, radiation, filtration or chemical methods using agents like alcohol, aldehydes, halogens and phenols. Effective sterilization requires the use of indicators to monitor the process and ensure it has adequately removed vegetative cells, spores and viruses. An ideal disinfecting agent is one that is fast-acting, compatible with other substances, non-toxic, and able to kill all types of microbes even in organic matter.
Killing or removing all forms of microbial life (including endospores) in a material or an object.
Mainly due to: oxidation of cell component, denature proteins, nucleic acids, RNA and loss of membrane permeability.
Procedures performed in a way to prevent contamination with infectious microorganisms
Used to prevent contamination of surgical instruments, medical personnel, and the patient during surgery
Sanitization: Lowering of microbial counts to prevent transmission in public setting (e.g., restaurants & public rest rooms)
Degerming: Mechanical removal of microbes from limited area. e.g., Alcohol swab on skin, washing of hands with soap
Sepsis: Bacterial contamination
Antisepsis: Reduction or Inhibition of microbes found on LIVING TISSUE
The document discusses various methods of sterilization including physical agents like heat, radiation and filtration, as well as chemical agents. It defines sterilization as a process that eliminates all microorganisms, while disinfection only destroys pathogenic organisms. Several sterilization techniques are described in detail, such as moist heat methods using steam under pressure in an autoclave, dry heat methods using hot air ovens, and chemical agents like alcohols, aldehydes, and dyes. The ideal properties of chemical disinfectants are also outlined.
This document provides definitions and information about various sterilization methods. It defines sterilization as a process that removes all microorganisms, while disinfection removes pathogenic organisms. The summary then discusses several physical sterilization methods like heat, filtration, radiation and ultrasonic/sonic vibrations. It also covers various chemical sterilization agents like alcohols, aldehydes, dyes, halogens, phenols, surface active agents, metallic salts and gases. For each method or agent, it provides details on their mechanism of action and typical uses.
Microorganisms can cause infection and are present everywhere. Sterilization aims to remove or destroy microorganisms from surfaces and materials. Various physical and chemical methods are used for sterilization with different effectiveness against bacterial vegetative cells, spores, viruses, and other microbes. Common physical sterilization methods include heat, radiation, and filtration, while chemical methods include alcohols, aldehydes, phenols, halogens, and other disinfecting agents. The choice of sterilization method depends on the type of material or substance being sterilized.
This document defines sterilization and disinfection, and discusses various physical and chemical agents used for these processes. It notes that sterilization aims to remove all microorganisms, while disinfection targets pathogenic organisms. Common physical agents for sterilization include heat, radiation, filtration and ultrasound. Chemical agents discussed include alcohols, aldehydes, dyes, halogens, phenols and gases. Proper sterilization requires consideration of multiple factors to effectively kill microbes.
This document provides definitions and outlines principles of sterilization and disinfection. It defines key terms like sterilization, disinfection, antiseptics, and antibiotics. It describes various physical agents (heat, radiation), chemical agents (gases, liquids), and mechanical removal methods used for microbial control. It discusses the mechanisms of action and structures of microbes targeted by sterilizers and disinfectants. It also provides details on specific processes like autoclaving, pasteurization, and chemical disinfectants like alcohols, aldehydes, halogens, and phenols.
Sterilization is a process that eliminates all microorganisms from an object or substance. It can be achieved through physical methods like heat, radiation, filtration or chemical methods using agents like alcohol, aldehydes, halogens and phenols. Effective sterilization requires the use of indicators to monitor the process and ensure it has adequately removed vegetative cells, spores and viruses. An ideal disinfecting agent is one that is fast-acting, compatible with other substances, non-toxic, and able to kill all types of microbes even in organic matter.
Killing or removing all forms of microbial life (including endospores) in a material or an object.
Mainly due to: oxidation of cell component, denature proteins, nucleic acids, RNA and loss of membrane permeability.
Procedures performed in a way to prevent contamination with infectious microorganisms
Used to prevent contamination of surgical instruments, medical personnel, and the patient during surgery
Sanitization: Lowering of microbial counts to prevent transmission in public setting (e.g., restaurants & public rest rooms)
Degerming: Mechanical removal of microbes from limited area. e.g., Alcohol swab on skin, washing of hands with soap
Sepsis: Bacterial contamination
Antisepsis: Reduction or Inhibition of microbes found on LIVING TISSUE
The document discusses various methods of sterilization including physical agents like heat, radiation and filtration, as well as chemical agents. It defines sterilization as a process that eliminates all microorganisms, while disinfection only destroys pathogenic organisms. Several sterilization techniques are described in detail, such as moist heat methods using steam under pressure in an autoclave, dry heat methods using hot air ovens, and chemical agents like alcohols, aldehydes, and dyes. The ideal properties of chemical disinfectants are also outlined.
This document provides definitions and information about various sterilization methods. It defines sterilization as a process that removes all microorganisms, while disinfection removes pathogenic organisms. The summary then discusses several physical sterilization methods like heat, filtration, radiation and ultrasonic/sonic vibrations. It also covers various chemical sterilization agents like alcohols, aldehydes, dyes, halogens, phenols, surface active agents, metallic salts and gases. For each method or agent, it provides details on their mechanism of action and typical uses.
Microorganisms can cause infection and are present everywhere. Sterilization aims to remove or destroy microorganisms from surfaces and materials. Various physical and chemical methods are used for sterilization with different effectiveness against bacterial vegetative cells, spores, viruses, and other microbes. Common physical sterilization methods include heat, radiation, and filtration, while chemical methods include alcohols, aldehydes, phenols, halogens, and other disinfecting agents. The choice of sterilization method depends on the type of material or substance being sterilized.
The document discusses various terms related to sterilization and disinfection including sterilization, disinfection, antiseptics, asepsis, and decontamination. It describes different methods of sterilization including physical methods like heat, radiation, filtration and drying as well as chemical methods using agents like alcohol, aldehydes, dyes, halogens, and phenols. Heat sterilization methods like moist and dry heat are explained in detail, noting the factors that influence sterilization and the appropriate temperatures and times required.
The document discusses various methods of sterilization and disinfection. It defines sterilization as the process of destroying all living microorganisms, including bacterial spores, while disinfection refers to reducing microorganisms to a safe level. Physical methods include heat, filtration, and radiation. Chemical methods utilize alcohols, aldehydes, phenols, and other agents. The ideal disinfectant is broad-spectrum, works in various conditions, and is non-toxic. Proper concentration, time, and temperature are important for effective sterilization and disinfection.
Dr. Ibrahim presented on various sterilization methods including physical agents like heat, radiation, and filtration as well as chemical agents like alcohols, aldehydes, and gases. The most common sterilization methods used in laboratories are moist heat using an autoclave at 121°C for 15 minutes or 134°C for 3 minutes, dry heat using a hot air oven at 160-180°C, and chemical methods using ethylene oxide or beta propiolactone gases. Proper testing of sterilization efficacy includes using chemical, mechanical, and biological indicators to confirm sterilizing conditions have been met.
Sterilization kills all microbes including spores, while disinfection kills most pathogens but not necessarily all spores. Sterilization methods include heat (dry heat, moist heat like autoclaving), radiation, filtration, gases (ethylene oxide), and plasma. Autoclaving at 121°C for 20 minutes is the most effective sterilization method. Other methods like dry heat, boiling, filtration and chemicals are used for heat-sensitive items or liquids. Proper monitoring of the sterilization process is important to ensure complete sterilization.
This document discusses sterilization and various sterilization methods. It defines sterilization as making something free from all microorganisms, including bacteria and spores. It then describes different terms used in sterilization like disinfection, antisepsis, and discusses physical sterilization methods like dry heat, moist heat and radiation. Chemical sterilization methods using agents like alcohol, aldehydes, dyes, halogens and phenols are also outlined. Finally, the document briefly discusses the mechanical sterilization method of passing solutions through filters to remove microorganisms.
This document discusses sterilization and microbial control. It defines sterilization as eliminating all transmissible agents, while disinfection reduces only pathogens. Contaminants can reduce productivity and degrade products. Various sterilization methods are covered, including heat (autoclaving, pasteurization, dry heat), filtration, freezing, radiation, chemicals, and gases. Effectiveness depends on factors like type and number of microbes, exposure time, and presence of organic material. Proper sterilization is important for controlling microbial growth in food, medical, and industrial applications.
Dr. Ibrahim presented on various sterilization methods including physical agents like heat, radiation, and filtration as well as chemical agents like alcohols, aldehydes, and gases. The most common sterilization methods used in laboratories are moist heat using an autoclave at 121°C for 15 minutes or 134°C for 3 minutes, dry heat using a hot air oven at 160-180°C, and chemical methods using ethylene oxide or beta propiolactone gases. Proper testing of sterilization efficacy includes using chemical, mechanical, and biological indicators to confirm sterilizing conditions have been met.
Sterilization and Disinfection
Sterilization kills all microorganisms including bacterial spores, while disinfection kills most pathogens excluding spores. Dry heat sterilizes through protein denaturation and moisture damage, while moist heat is more effective through coagulation and protein denaturation. Autoclaving at 121°C for 15 minutes is the most effective sterilization method. Membrane filtration below 0.45 μm is used to remove microbes from heat-labile liquids. Disinfectants like alcohol, aldehydes, phenol, hydrogen peroxide, ethylene oxide and halogens are used to disinfect surfaces, with alcohols and aldehydes being
1. The document discusses various methods for disinfecting and controlling microbial growth, including physical methods like heat and radiation, and chemical methods like disinfectants, antiseptics, and other antimicrobial agents.
2. It describes the mechanisms of action for different antimicrobial agents like phenolics, iodophores, alcohols, aldehydes, and oxidizing agents which act by damaging cell membranes, proteins, and nucleic acids.
3. The effectiveness of disinfection depends on factors like concentration and contact time of the antimicrobial agent, amount of organic matter present, and characteristics of the microbes being targeted.
The document discusses disinfection, providing definitions and outlining factors that determine a disinfectant's potency. It describes ideal characteristics for disinfectants and lists common chemical agents used, including their mechanisms and uses. Different levels of disinfection are defined. Specific disinfectants like alcohols, aldehydes, dyes, halogens and phenols are explained in detail. Methods for disinfecting various surfaces and materials are provided.
Sterilization and disinfection are both crucial processes for controlling the spread of harmful microorganisms. Sterilization eliminates all forms of microbial life, including bacteria, viruses, and spores, while disinfection reduces the number of pathogenic microorganisms to a level that is considered safe for public health. Sterilization typically involves more rigorous methods, such as heat, chemicals, or radiation, while disinfection can often be achieved with disinfectants like bleach or alcohol.
Surgical asepsis, sterilization and disinfectionGangaYadav4
This document defines key terms related to asepsis and infection control such as sepsis, asepsis, antisepsis, disinfectants, and sterilization. It describes various methods of sterilization including physical sterilization using dry heat, moist heat and autoclaving. Chemical sterilization using agents like alcohols, aldehydes, chlorhexidine, and iodine is also discussed. Gas sterilization using ethylene oxide and irradiation sterilization techniques are summarized. Principles of surgical asepsis including maintaining a sterile field and proper preparation of surgical personnel and patients are highlighted in brief.
This document discusses sterilization and disinfection techniques used to eliminate microorganisms. It defines key terms and outlines various methods for sterilizing instruments and disinfecting surfaces, including heat, chemicals, gases, and filtration. Effective sterilization and disinfection requires understanding the microbial characteristics and selecting the appropriate process for different medical equipment, environments, and situations.
This document discusses various methods of sterilization and disinfection. It defines key terms and lists factors that affect the efficacy of sterilization. It describes the decreasing order of microbial resistance and then explains different sterilization methods like heat, filtration, radiation, and chemicals. Heat methods include dry heat (flaming, oven) and moist heat (boiling, autoclaving). Chemical methods discussed are alcohols, aldehydes, phenols, and halogens. The document also covers different disinfectants and factors influencing their activity. Finally, it lists examples of hospital disinfection methods.
This document discusses various methods of sterilization including physical and chemical agents. Physical agents include dry heat (hot air ovens, flaming, incineration), moist heat (pasteurization, boiling, steam), filtration, radiation, and ultrasound. Moist heat via autoclaving is the most reliable sterilization method. Chemical agents discussed are alcohols, aldehydes, dyes, halogens, phenols, and gases. The document defines key sterilization terms and explains the mechanisms of different sterilization methods.
Sterilization and disinfection of environment and instruments by dr shireen ...Hassan Ahmad
This document discusses cleaning and disinfection in healthcare settings. It describes various pathogens that can be found on medical equipment and surfaces. It outlines important areas that require cleaning like nurseries, operating rooms, and floors. It discusses the factors involved in effective cleaning and lists some commonly used disinfectants like sodium hypochlorite, phenols, and iodine. It also describes the differences between disinfection, antisepsis, and sterilization. Autoclaving is highlighted as the most effective sterilization method using high temperatures and pressure.
sulu sterilisation ppt amended 28 nov last and final.pptxmanojyadav4516
This document discusses the history and methods of sterilization and disinfection. It notes that sterilization was developed partly for food preservation and to prevent infection by bacteria. Key developments included Nicolas Appert using heat in 1809, Louis Pasteur inventing pasteurization in 1865, and Charles Chamberland inventing the pressure steam sterilizer (autoclave) in 1879. The document defines sterilization, disinfection, and other related terms. It describes various physical methods like heat and chemical methods like alcohols, aldehydes, and gases that can be used to kill microorganisms. Different classes of medical devices and the most resistant and least resistant types of microbes are also discussed.
Travel Clinic Cardiff: Health Advice for International TravelersNX Healthcare
Travel Clinic Cardiff offers comprehensive travel health services, including vaccinations, travel advice, and preventive care for international travelers. Our expert team ensures you are well-prepared and protected for your journey, providing personalized consultations tailored to your destination. Conveniently located in Cardiff, we help you travel with confidence and peace of mind. Visit us: www.nxhealthcare.co.uk
The document discusses various terms related to sterilization and disinfection including sterilization, disinfection, antiseptics, asepsis, and decontamination. It describes different methods of sterilization including physical methods like heat, radiation, filtration and drying as well as chemical methods using agents like alcohol, aldehydes, dyes, halogens, and phenols. Heat sterilization methods like moist and dry heat are explained in detail, noting the factors that influence sterilization and the appropriate temperatures and times required.
The document discusses various methods of sterilization and disinfection. It defines sterilization as the process of destroying all living microorganisms, including bacterial spores, while disinfection refers to reducing microorganisms to a safe level. Physical methods include heat, filtration, and radiation. Chemical methods utilize alcohols, aldehydes, phenols, and other agents. The ideal disinfectant is broad-spectrum, works in various conditions, and is non-toxic. Proper concentration, time, and temperature are important for effective sterilization and disinfection.
Dr. Ibrahim presented on various sterilization methods including physical agents like heat, radiation, and filtration as well as chemical agents like alcohols, aldehydes, and gases. The most common sterilization methods used in laboratories are moist heat using an autoclave at 121°C for 15 minutes or 134°C for 3 minutes, dry heat using a hot air oven at 160-180°C, and chemical methods using ethylene oxide or beta propiolactone gases. Proper testing of sterilization efficacy includes using chemical, mechanical, and biological indicators to confirm sterilizing conditions have been met.
Sterilization kills all microbes including spores, while disinfection kills most pathogens but not necessarily all spores. Sterilization methods include heat (dry heat, moist heat like autoclaving), radiation, filtration, gases (ethylene oxide), and plasma. Autoclaving at 121°C for 20 minutes is the most effective sterilization method. Other methods like dry heat, boiling, filtration and chemicals are used for heat-sensitive items or liquids. Proper monitoring of the sterilization process is important to ensure complete sterilization.
This document discusses sterilization and various sterilization methods. It defines sterilization as making something free from all microorganisms, including bacteria and spores. It then describes different terms used in sterilization like disinfection, antisepsis, and discusses physical sterilization methods like dry heat, moist heat and radiation. Chemical sterilization methods using agents like alcohol, aldehydes, dyes, halogens and phenols are also outlined. Finally, the document briefly discusses the mechanical sterilization method of passing solutions through filters to remove microorganisms.
This document discusses sterilization and microbial control. It defines sterilization as eliminating all transmissible agents, while disinfection reduces only pathogens. Contaminants can reduce productivity and degrade products. Various sterilization methods are covered, including heat (autoclaving, pasteurization, dry heat), filtration, freezing, radiation, chemicals, and gases. Effectiveness depends on factors like type and number of microbes, exposure time, and presence of organic material. Proper sterilization is important for controlling microbial growth in food, medical, and industrial applications.
Dr. Ibrahim presented on various sterilization methods including physical agents like heat, radiation, and filtration as well as chemical agents like alcohols, aldehydes, and gases. The most common sterilization methods used in laboratories are moist heat using an autoclave at 121°C for 15 minutes or 134°C for 3 minutes, dry heat using a hot air oven at 160-180°C, and chemical methods using ethylene oxide or beta propiolactone gases. Proper testing of sterilization efficacy includes using chemical, mechanical, and biological indicators to confirm sterilizing conditions have been met.
Sterilization and Disinfection
Sterilization kills all microorganisms including bacterial spores, while disinfection kills most pathogens excluding spores. Dry heat sterilizes through protein denaturation and moisture damage, while moist heat is more effective through coagulation and protein denaturation. Autoclaving at 121°C for 15 minutes is the most effective sterilization method. Membrane filtration below 0.45 μm is used to remove microbes from heat-labile liquids. Disinfectants like alcohol, aldehydes, phenol, hydrogen peroxide, ethylene oxide and halogens are used to disinfect surfaces, with alcohols and aldehydes being
1. The document discusses various methods for disinfecting and controlling microbial growth, including physical methods like heat and radiation, and chemical methods like disinfectants, antiseptics, and other antimicrobial agents.
2. It describes the mechanisms of action for different antimicrobial agents like phenolics, iodophores, alcohols, aldehydes, and oxidizing agents which act by damaging cell membranes, proteins, and nucleic acids.
3. The effectiveness of disinfection depends on factors like concentration and contact time of the antimicrobial agent, amount of organic matter present, and characteristics of the microbes being targeted.
The document discusses disinfection, providing definitions and outlining factors that determine a disinfectant's potency. It describes ideal characteristics for disinfectants and lists common chemical agents used, including their mechanisms and uses. Different levels of disinfection are defined. Specific disinfectants like alcohols, aldehydes, dyes, halogens and phenols are explained in detail. Methods for disinfecting various surfaces and materials are provided.
Sterilization and disinfection are both crucial processes for controlling the spread of harmful microorganisms. Sterilization eliminates all forms of microbial life, including bacteria, viruses, and spores, while disinfection reduces the number of pathogenic microorganisms to a level that is considered safe for public health. Sterilization typically involves more rigorous methods, such as heat, chemicals, or radiation, while disinfection can often be achieved with disinfectants like bleach or alcohol.
Surgical asepsis, sterilization and disinfectionGangaYadav4
This document defines key terms related to asepsis and infection control such as sepsis, asepsis, antisepsis, disinfectants, and sterilization. It describes various methods of sterilization including physical sterilization using dry heat, moist heat and autoclaving. Chemical sterilization using agents like alcohols, aldehydes, chlorhexidine, and iodine is also discussed. Gas sterilization using ethylene oxide and irradiation sterilization techniques are summarized. Principles of surgical asepsis including maintaining a sterile field and proper preparation of surgical personnel and patients are highlighted in brief.
This document discusses sterilization and disinfection techniques used to eliminate microorganisms. It defines key terms and outlines various methods for sterilizing instruments and disinfecting surfaces, including heat, chemicals, gases, and filtration. Effective sterilization and disinfection requires understanding the microbial characteristics and selecting the appropriate process for different medical equipment, environments, and situations.
This document discusses various methods of sterilization and disinfection. It defines key terms and lists factors that affect the efficacy of sterilization. It describes the decreasing order of microbial resistance and then explains different sterilization methods like heat, filtration, radiation, and chemicals. Heat methods include dry heat (flaming, oven) and moist heat (boiling, autoclaving). Chemical methods discussed are alcohols, aldehydes, phenols, and halogens. The document also covers different disinfectants and factors influencing their activity. Finally, it lists examples of hospital disinfection methods.
This document discusses various methods of sterilization including physical and chemical agents. Physical agents include dry heat (hot air ovens, flaming, incineration), moist heat (pasteurization, boiling, steam), filtration, radiation, and ultrasound. Moist heat via autoclaving is the most reliable sterilization method. Chemical agents discussed are alcohols, aldehydes, dyes, halogens, phenols, and gases. The document defines key sterilization terms and explains the mechanisms of different sterilization methods.
Sterilization and disinfection of environment and instruments by dr shireen ...Hassan Ahmad
This document discusses cleaning and disinfection in healthcare settings. It describes various pathogens that can be found on medical equipment and surfaces. It outlines important areas that require cleaning like nurseries, operating rooms, and floors. It discusses the factors involved in effective cleaning and lists some commonly used disinfectants like sodium hypochlorite, phenols, and iodine. It also describes the differences between disinfection, antisepsis, and sterilization. Autoclaving is highlighted as the most effective sterilization method using high temperatures and pressure.
sulu sterilisation ppt amended 28 nov last and final.pptxmanojyadav4516
This document discusses the history and methods of sterilization and disinfection. It notes that sterilization was developed partly for food preservation and to prevent infection by bacteria. Key developments included Nicolas Appert using heat in 1809, Louis Pasteur inventing pasteurization in 1865, and Charles Chamberland inventing the pressure steam sterilizer (autoclave) in 1879. The document defines sterilization, disinfection, and other related terms. It describes various physical methods like heat and chemical methods like alcohols, aldehydes, and gases that can be used to kill microorganisms. Different classes of medical devices and the most resistant and least resistant types of microbes are also discussed.
Travel Clinic Cardiff: Health Advice for International TravelersNX Healthcare
Travel Clinic Cardiff offers comprehensive travel health services, including vaccinations, travel advice, and preventive care for international travelers. Our expert team ensures you are well-prepared and protected for your journey, providing personalized consultations tailored to your destination. Conveniently located in Cardiff, we help you travel with confidence and peace of mind. Visit us: www.nxhealthcare.co.uk
Adhd Medication Shortage Uk - trinexpharmacy.comreignlana06
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
Travel vaccination in Manchester offers comprehensive immunization services for individuals planning international trips. Expert healthcare providers administer vaccines tailored to your destination, ensuring you stay protected against various diseases. Conveniently located clinics and flexible appointment options make it easy to get the necessary shots before your journey. Stay healthy and travel with confidence by getting vaccinated in Manchester. Visit us: www.nxhealthcare.co.uk
Does Over-Masturbation Contribute to Chronic Prostatitis.pptxwalterHu5
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
5-hydroxytryptamine or 5-HT or Serotonin is a neurotransmitter that serves a range of roles in the human body. It is sometimes referred to as the happy chemical since it promotes overall well-being and happiness.
It is mostly found in the brain, intestines, and blood platelets.
5-HT is utilised to transport messages between nerve cells, is known to be involved in smooth muscle contraction, and adds to overall well-being and pleasure, among other benefits. 5-HT regulates the body's sleep-wake cycles and internal clock by acting as a precursor to melatonin.
It is hypothesised to regulate hunger, emotions, motor, cognitive, and autonomic processes.
Cell Therapy Expansion and Challenges in Autoimmune DiseaseHealth Advances
There is increasing confidence that cell therapies will soon play a role in the treatment of autoimmune disorders, but the extent of this impact remains to be seen. Early readouts on autologous CAR-Ts in lupus are encouraging, but manufacturing and cost limitations are likely to restrict access to highly refractory patients. Allogeneic CAR-Ts have the potential to broaden access to earlier lines of treatment due to their inherent cost benefits, however they will need to demonstrate comparable or improved efficacy to established modalities.
In addition to infrastructure and capacity constraints, CAR-Ts face a very different risk-benefit dynamic in autoimmune compared to oncology, highlighting the need for tolerable therapies with low adverse event risk. CAR-NK and Treg-based therapies are also being developed in certain autoimmune disorders and may demonstrate favorable safety profiles. Several novel non-cell therapies such as bispecific antibodies, nanobodies, and RNAi drugs, may also offer future alternative competitive solutions with variable value propositions.
Widespread adoption of cell therapies will not only require strong efficacy and safety data, but also adapted pricing and access strategies. At oncology-based price points, CAR-Ts are unlikely to achieve broad market access in autoimmune disorders, with eligible patient populations that are potentially orders of magnitude greater than the number of currently addressable cancer patients. Developers have made strides towards reducing cell therapy COGS while improving manufacturing efficiency, but payors will inevitably restrict access until more sustainable pricing is achieved.
Despite these headwinds, industry leaders and investors remain confident that cell therapies are poised to address significant unmet need in patients suffering from autoimmune disorders. However, the extent of this impact on the treatment landscape remains to be seen, as the industry rapidly approaches an inflection point.
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
2. Sterilization:
Process by which an article, surface or medium is freed of all living
micro organisms either in vegetative or spore state
Disinfection:
The destruction or removal of all pathogenic organisms capable of
giving rise to infection
Rutala WA, Weber DJ. Guideline for disinfection and sterilization in healthcare facilities, 2008.
3. Antisepsis:
The term is used to indicate the prevention of infection , usually by
inhibiting the growth of bacteria in wound or tissues
Bactericidal agents: those who are able to kill
bacteria
Bacteriostatic agents: only prevention of multiplication of bacteria, but
they remain alive
4. Cleaning:
Important preparatory step before sterilisation or disinfection , by
removing soil and other dirty.
Decontamination:
The process of rendering an article or area free of contamination ,
including microbial, chemical, radioactive and other hazards
5. Sterilisation
Physical agents:
Drying
Dry heat: by flaming, incineration or hot air
Moist heat: pasteurization , boiling, steam under pressure.
Filtration: candle, asbestos pads, membranes
Radiation
Ultrasonic and sonic vibration
7. Drying:
Moisture is essential for growth of bacteria
Drying in air has deleterious effects on many
bacteria
Spores are unaffected.
8. Heat:
Most reliable method of sterilization and should be the method of
choice.
The factors influencing sterilisation by heat:
Nature of heat dry or moist
Temperature and time
Number of microorganisms present
Type of material from which organism have to be eliminated
9. Mode of action
Dry heat : protein denaturation, oxidative damage and toxic
effects of elevated level of electrolytes.
Moist heat: denaturation and coagulation of proteins.
10. Dry heat
1. Flaming: inoculating loop/ wire , tip of forceps and searing
spatulas
2. Incineration: This is excellent method for terminal
sterilisation for biomedical waste. e,g; contaminated cloth,
animal waste and pathological materials
11.
12. Hot air oven:
Holding time: 160°c for 2 hours
uses: glassware, forceps, scissors, scalpels, swab, liquid paraffin, and
fats.
Sterilisation control: spores of clostridium tetani
13.
14. Moist heat:
Moist heat can be categorized into 3 groups
I. Temperature below 100°c
II. Temperature at 100°c
III. Temperature above 100°c
15. Temperature below 100°c
Pasteurisation of milk:
Holding period: 63°c, 30 min or 72°c, 15- 20 min
Target : all nonsporing pathogens
eg: mycobacteria, brucellae, salmonella
16. Temperature at 100°c
Boiling
Not recommended for sterilising but ok for disinfection.
Sterilisation may be promoted by addition of sodium bicarbonate
to the water
Holding period: 30 min
17. Temperature above 100°c
Autoclave (steam sterilizer):
Principle: water boils when its vapour pressure equals the
surrounding atmosphere.
Thus when pressure inside closed vessels increases the
temperature at which water boils increases too.
Flash autoclave: 134°c @ 30psi for 3min
High speed autoclave: 121°c @15psi for 15 min
21. Filtration
Help to remove bacteria from heat labile liquids
uses : sera and solution of sugar or antibiotics
Principle: as viruses pass through the ordinary filters, filtration
can be used to obtain bacteria free filtration of clinical sample for
virus isolation.
22. Radiation
2 type of radiation
Ionising : gamma rays , high energy electrons
Non -ionising : infrared, UV rays
Gamma rays :
uses: Plastics, syringes, swabs, catheters, animal feeds, oils,
greases.
23.
24. Infrared:
Used for rapid mass sterilisation of prepacked items; syringe,
catheters
UV rays:
Disinfect enclosed area such as entryways, operation theatres and
labs.
25. Ultrasonic and sonic vibration :
High frequency sound waves above 15000 cycle per second can
cause cavitation formation and denaturation of bacterial cell
membranes
Effective bactericidal and germicidal
Ultra sonic vibration act as cleaning agent also.
26. CHEMICALAGENTS
Ideal antiseptic or disinfectant:
Effective against all microorganisms
Be active in presence of organic matter
Effective in acidic as well as in alkaline media
Have speedy action
Be stable
Have high penetrating power
Rutala WA, Weber DJ. Guideline for disinfection and sterilization in healthcare facilities, 2008.
27. Mode of action:
Protein coagulation
Disruption of cell membrane resulting in exposure, damage/ loss
of content
Removal of sulfhydryl group which is essential for normal
functioning of enzyme
Substrate competition
28. Factors that determine the potency of disinfectants:
Concentration of substance
Time of action
pH of the medium
Temperature
Nature of organisms
Presence of extraneous material
29. Alcohol
Frequently used:
Ethyl alcohol
Isopropyl alcohol
Must be used at concentration 60-90%
Mode of action: protein denaturation and coagulation
Rapidly bactericidal rather than bacteriostatic against vegetative
forms of bacteria
30. Also tuberculocidal, fungicidal, and virucidal but do not destroy
bacterial spores
Bactericidal activity drops sharply when diluted below 50%
concentration
Alcohol is used effectively to disinfect oral and rectal
thermometers, scissors and stethoscopes
Isopropyl alcohol was slightly more bactericidal than ethyl
alcohol.
Jolivet et all . Surgical field and skin preparation. Orthopaedics & Traumatology: Surgery &
Research. 2019 ;105(1):S1-6.
31.
32. Aldehyde
Formaldehyde:
Bactericidal , sporicidal and has lethal effect on viruses
Used to preserve anatomical specimens, destroying spores of
anthrax on hair and wool.
Potential carcinogen
Exposure to low level cause asthma like respiratory problems,
skin irritation.
33. Gluteraldehyde:
Effective against tubercle bacilli, fungi, viruses
Less toxic and irritant to eyes, skin
Used to treat corrugated rubber ,anaesthetic rubber , face masks,
plastic ET tube, metal instruments and polythene tubing.
34. Orthophthaladehyde(OPA)
Mode of action :
OPA and glutaraldehyde interact with amino acids, proteins, and
microorganisms
OPA appears to kill spore by blocking the spore germination
process
Microbicidal activity
Costly
35. Cidex: 2.4% glutaraldehyde or 0.55% orthophthaldehyde
Minimum time for immersion 12 min at 25°c
Reuse period is 14 days
36. Dyes
2 groups of dyes:
Aniline dye
Acridine dye
Both are bacteriostatic in high dilution but are of low bactericidal
activity.
Aniline dye is more active aganist gram +ve than gram –ve organisms
Used in microbiology labs as selective agents in culture media.
37. Halogens
Iodine:
Skin disinfectant
Povidone iodine releasing slowly and minimize toxicity
Time required for action iodine 30sec to 2 min
MOA: iodinates of lipids and oxidation of cellular componets.
Active bactericidal ,moderate action on spores.
38.
39. Chlorine:(hypochlorite's)
The most widely used of the chlorine disinfectants
They have a broad spectrum of antimicrobial activity
Inexpensive and fast acting
Remove dried or fixed organisms and biofilms
Uses in floor, blood spills .
Bleaching powder for water supplies, swimming pools and food
and dairy industries.
40. Phenols
Obtained from distillation of coal tar between 170-270°c.
Lethal effect: capacity to cause cell membrane damage releasing
cell contents and causing lysis.
Carbolic acid is widely used in as disinfectants in hospital
Carbolization :5% carbolic acid for 20 min
41. Gases
Types of gases
Ethylene oxide
Formaldehyde gas
Beta propiolactone(BPL)
Ethylene oxide:
Action is due to its alkylating the amino, carboxyl, hydroxyl and
sulphydryl groups in protein molecules .also on DNA and RNA
43. Formaldehyde gas:
Employed for fumigation of OT .
After fumigation, the doors should be sealed and left unopened
for 48 hours
44. Beta propiolactone:
Product of ketone and formaldehyde with a boiling point of 163°c.
Rapid biocidal activity but carcinogenic
Capable of killing all microorganisms and is very active against
viruses
45. Surface active agents
Substance that alter the energy relation relationship at interfaces,
producing a reduction in surgfacetension
Widely used as wetting agents, detergents and emulsifiers
46. Metallic salts
Salt of heavy metals have greater action
Eg: salt of sliver , copper and mercury
Protein coagulant and have capacity to combine with free
sulhydry group of cell enzymes.
47. Hydrogen peroxide
MOA: production of hydroxyl free radical that can attack
membrane lipid ,DNA and other essential cell componets.
Bactericidal, virucidal, sporicidal and fungicidal properties
Synergistic sporicidal effects of hydrogen peroxide (5.9%-23.6%)
and acetic acid
Use :effective disinfectant when used on inanimate surfaces
48. Hydrogen peroxide gas plasma:
Low temperature hydrogen peroxide plasma
Short duration time (45 min/cycle)
Low temperature and humidity , no toxic , no chemical residues
Items : laparoscopic instrument, plastic , sutures ets
49.
50. Peracetic acid :
•MOA: Protein denaturation , disrupts cell wall
permeability and oxidizes sulfhydral bonds in protein
,enzymes and other metabolites
•Concentration 0.25% for 12 min at 50ºc.
•Reuse within 24 hr
•Uses: endoscopes and bronchoscopes
51. Spaulding classification :surgical/ medical instruments
based on risk of infection
Critical devices:
enter normally sterile body tissue or vascular system: e.g surgical
instruments, implants, cardiac and urinary catheters.
Sterilisation: ethyline oxide or hydrogen peroxide gas plasma
Semi critical devices:
contact mucous membranes or nonintact skin: e.g: endoscopes,
laryngoscope.
Sterilisation: glutaraldehyde, hydrogen peroxide, ortho phthaladehyde,
peracetic acid.
55. Sterilization /disinfectant
method
Contact time Application
Alcohol
(chlorehexidine)
2% or 4%+ 70%
isopropyl alcohol(30 sec
to 2min)
Surgical scrub and skin
preparation, hand
hygiene and
thermometers,
sphygmomanometer
Gluteraldehyde 2.4% for minmum
12min at 25°c
Metal instruments
Povidone iodine 5%, 7.5% and 10%(30
sec to 2 min)
Skin preparation, scrub
and wound cleaning
Chlorine (hypochlorite) 5.25%- 6.15%. 12min Blood spillage, Floors,
ICU and wards
Carbolic acid(carbolization) 5% carbolic acid for 20
min
OT , ICU
Ethylene oxide Sutures, endoscopic
devices
Hydrogen peroxide gas
plasma
45 min Laparoscopic
instruments, plastic and
sutures
56. Sterilization /disinfectant
method
Contact time Application
Autoclave Flash:134°c@30 psi for
3min
High speed:121°@ 15psi
for 15 min
Metal instrument,
dressing pads
Gamma radiation Sutures, syringes,
catheters