Downloaded 46 times
More Related Content
Similar to Amniotic fluid
Amniotic fluid
- 1. Amniotic fluid analysis DrRohini C Sane Professor Department of Biochemistry DY Patil Medical College ,EBENE
- 2. Amniotic fluid analysis •Liquid produced by membranes & fetus • Surrounds the fetus throughout pregnancy • Volume –increases with gestational age( 2weeks –30ml to 350ml (20weeks )---thereafter 500-1000ml • Clear with some desquamated fetal cells & little lipid
- 3. Functions of Amnioticfluid • It provides physical protection • Medium for exchange of various chemicals
- 4. Diagnostic importance ofamniotic fluid • AMNIOCENTESIS –PROCESS BY WHICH AMNIOTIC FLUID COLLECTED FOR ANALYSIS • Assessment of fetal maturity—cytological staining of fat cells Estimation of creatinine concentration - >1.6mg /dl indicates fetal maturity Lung maturity –lecithin –sphingomyelin (l/s) ratio > 2:1 or more indicates normal Lung maturity lecithin –sphingomyelin (l/s) ratio < 2:1 Lung immature Delay induced delivery till Lung mature INDEX OF SURFACTANT –(SURFACE LOWERING COMPLEX) PREGNANCY ----CELLS LINING ALVEOLI START SYNTHESIZING DIPALMIOTYL LECITHIN,AS LUNG MATURE L/S RATIO INCREASES
- 5. EARLY GESTATION PRETERM VLOUME 450-1200 ML 500-1400ML BILIRUBIN < 0.O75 MG/ML < 0.O25 MG/ML CREATININE 0.8—1.1 MG/DL 1.8-4.0 MG/DL ESTIROL 10 MICROGRAM /DL >60 MICROGRAM /DL LECITHIN – SPHINGOMYLIN RATIO < 1:1 > 2:1 PROTEIN 0.6 -0.24 GM/DL 0.26 -0.19 GM/DL UREA 18+6MG/DL 30+11MG/DL URIC ACID 3.7+1 MG/DL 9.9 +2.2 MG/DL
- 6. Diagnostic importance ofamniotic fluid • Diagnosis of congenital disorder • prenatal diagnosis Chromosomal disorder Down”s syndrome Metabolic disorder Cystic fibrosis Sex linked disorder Haemophillia Enzyme defect Tay sach”s disease
- 7. Diagnostic importance ofamniotic fluid • 22-24 WKS OF GESTATION –ESTIMATION OF CHOLINE ESTERASE ISOENZYME ---NEURAL TUBE DEFECTS • PHENYL ALANINE –PHENYL KETONURIA • METHYL MALONIC ACID –VITAMIN B12 DEFICIECY –INBORN DEFECT ODD CHAIN FATTY ACID)VALINE,ISOLEUCINE,THREONINE
- 8. Diagnostic importance ofamniotic fluid • ASSESSMENT OF HEMOLYTIC DISEASE—ESTIMATION OF BILIRUBIN TO EVALUATE SEVERITY OF HEMOLYTIC DISEASE • MEASUREMENT OF ALPHA FETO PROTEIN –(AFP) • NORMAL ---15-40 MICROGRAMS /ML • < 1.0 MICROGRAMS /ML AT 40WEEKS OF GESTATION ---NEURAL TUBE DEFECT,FETAL DISTRESS ,TURNER”S SYNDROME
- 9.
- 12.
- 13.