it will help you to understand how the protein microarrays are made, what are the different types and what all purposes they are used for. its very useful ppt
This slide summarises the recent advancement in the field of protein Array. It includes a cell free expression system, Nucleic acid programmable protein array, nano-well array and other techniques.
Co-IP is a commonly used technique to identify physiologically-relevant protein-protein interactions. To be specific, utilizing antibodies to capture proteins that are bound to this specific target protein. https://www.creative-proteomics.com/services/co-immunoprecipitation-co-ip.htm
it will help you to understand how the protein microarrays are made, what are the different types and what all purposes they are used for. its very useful ppt
This slide summarises the recent advancement in the field of protein Array. It includes a cell free expression system, Nucleic acid programmable protein array, nano-well array and other techniques.
Co-IP is a commonly used technique to identify physiologically-relevant protein-protein interactions. To be specific, utilizing antibodies to capture proteins that are bound to this specific target protein. https://www.creative-proteomics.com/services/co-immunoprecipitation-co-ip.htm
Homology modeling, also known as comparative modeling of protein, refers to constructing an atomic-resolution model of the "target" protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein.
Yeast two-hybrid is based on the reconstitution of a functional transcription factor (TF) when two proteins or polypeptides of interest interact. Upon interaction between the bait and the prey, the DBD and AD are brought in close proximity and a functional TF is reconstituted upstream of the reporter gene.
The de novo peptide sequencing is a method for peptide sequencing performed without prior knowledge of the amino acid sequence. It uses computational approaches to deduce the sequence of peptide directly from the experimental MS/MS spectra.This method can obtain the peptide sequences without a protein database. It can be used for un-sequenced organisms, antibodies, peptides with posttranslational modifications, and endogenous peptides.
Homology modeling, also known as comparative modeling of protein, refers to constructing an atomic-resolution model of the "target" protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein.
Yeast two-hybrid is based on the reconstitution of a functional transcription factor (TF) when two proteins or polypeptides of interest interact. Upon interaction between the bait and the prey, the DBD and AD are brought in close proximity and a functional TF is reconstituted upstream of the reporter gene.
The de novo peptide sequencing is a method for peptide sequencing performed without prior knowledge of the amino acid sequence. It uses computational approaches to deduce the sequence of peptide directly from the experimental MS/MS spectra.This method can obtain the peptide sequences without a protein database. It can be used for un-sequenced organisms, antibodies, peptides with posttranslational modifications, and endogenous peptides.
his PowerPoint presentation provides a thorough exploration of bioactive compounds and extraction methods, ensuring audiences gain a comprehensive understanding. From the fundamentals to advanced techniques, it covers key concepts, processes, and applications, empowering viewers with essential knowledge in this vital field of study.
microspheres,types, advantages and disadvantages,methods of preparation, evaluation or characterization of microspheres and applications of microspheres in various pharmaceutical fields.
Marine natural products: General methods of isolation and
purification, Study of Marine toxins, Recent advances in research
in marine drugs, Problems faced in research on marine drugs
such as taxonomical identification, chemical screening and their
solution.
a brief summary of antibacterial assays performed in lab studies to find out the dosing and efficacy of drugs and compounds on bacterial effect. different methods used are described alongwith imaged to give you a good idea.
HSSC Second year Chemistry course slides for Federal Board Pakistan, lectures by Dr. Raja Hashim Ali (also available on Youtube as a series of video lectures).
https://www.youtube.com/playlist?list=PLCfCZszhGHBffeBtwpqkxATBDRkdJsOkn
Isolation of organelles is accomplished by cell membrane lysis and density gradient centrifugation to separate organelles from contaminating cellular structures. Intact nuclei and organelles have distinctive sizes in mammalian cells, enabling them to be separated by this method.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
1. By group No 8
Affinity Purification and Mass
Spectrophotometry
2. Group
Members
1. Shahid Maqbool 1025
2. Chand 1004
3. Abu-Bakr Saddique 1015
4. Fahad Raza 5007
5. Talha Ilyas 5006
6. Ali Usman 1034
7. M. Mubashar 1046
3. Affinity Purification
A biochemistry technique used to isolate and purify specifc
biomolecules, like proteins or nucleic acids, based on their
specific binding affnity to a known ligand or molecule.
Discovery
Discovered by Dr. Herbert Boyer and Dr. Stanley Cohen in the
early 1970s.
4. Purpose
This method allows for selective and effcient extraction of the
desired molecule from complex mixtures, facilitating further
analysis, characterization, or use in various applications like
research or medical diagnostics.
5. Components
There are three components of purification.
1. Matrix/Support
2. Affinity Ligand
3. Buffer System
8. Applications
• Separation of mixture of compounds.
• Removal of impurities or in purification process.
• Detection of substrates
• Investigation of binding sites of enzymes
• In in vitro antigen-antibody reactions
• Detection of Single Nucleotide polymorphisms and mutations
in nucleic acids
9. Advantages
• High specificity
• Target molecules can be obtained in a highly pure state
• Single step purification
• The matrix can be reused rapidly.
• The matrix is a solid, can be easily washed and dried.
• Give purified product with high yield.
10. Limitations
• Time consuming method.
• May be expensive.
• Intense labour
• Non-specific adsorption cannot be totally eliminated
• Limited availability and high cost of immobilized ligands.
• Proteins get denatured if required pH is not adjusted.
11. Mass Spectrometry
• A technique used to analyze the mass-to-charge ratio of ions.
• A powerful tool in analytical chemistry and biochemistry for
identifying and quantifying molecules based on their mass.
12. Discovery
Mass spectrometry began with J.J. Thomson in 1913, and the
modern era saw key developments, including the invention of
the quadrupole mass filter in the 1950.
14. Applications
• Used in various scientific disciplines, including
chemistry, biochemistry, environmental science, and
medicine.
• For tasks such as identifying unknown compounds,
determining molecular structures, and studying
biomolecules.
15. AP and MS
• Affinity purification is often coupled with mass spectrometry
(MS) in a technique called affinity-based mass spectrometry.
• After isolating a target biomolecule using affinity purification,
mass spectrometry is employed to analyze and identify the
purified molecules based on their mass-to-charge ratios.
