This document discusses high-performance thin layer chromatography (HPTLC). HPTLC works on the same principles as thin layer chromatography, using adsorption and mobile phase flow through capillary action to separate analytes according to their affinity for the stationary phase. The document describes the instrumentation of HPTLC, including parts like the applicator and development chamber. It also outlines the experimental procedure and applications of HPTLC in fields like pharmaceuticals and food testing. Advantages include high resolution, sensitivity and reproducibility, while disadvantages include short separation beds and limited sample numbers per plate. HPTLC is compared to traditional thin layer chromatography.

1. HIGH-PERFORMANCE THIN LAYER
CHROMATOGRAPHY
Mr K V NANDA KUMAR
Associate Professor,
Department of Pharmaceutical Analysis,
Krishna Teja Pharmacy College, Tirupati-517506

2. CONTENTS
INTRODUCTION
PRINCIPLE
INSTRUMENTATION
WORKING PROCEDURE
TLC VS HPTLC
APPLICATIONS
REFERNECES

4. INTRODUCTION
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5. P R I N C I P L E
The HPTLC works on the same principles as TLC such
as the principle of separation is adsorption.
The mobile phase or solvent flows through the capillary
action. The analytes move according to their affinities
towards the stationary phase (adsorbent).
The higher affinity component travels slower towards
the stationary phase.
A low-affinity component travels rapidly toward the
stationary phase.
On a chromatographic plate, then, the components are
separated.

6. HPTLC INSTRUMENT
PARTS: HOW AND
WHAT THEY DO
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7. EXPERIMENTAL PROCEDURE FOR HPTLC
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8. CONTIUED….
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9. HIGH-
PERFORMANCE
THIN-LAYER
CHROMATOGRAPHY
(HPTLC)
APPLICATIONS
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10. THE ADVANTAGES
OF HPTLC ARE AS
FOLLOWS.
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11. T H E D I S A D VA N TA G E S O F H P T L C A R E A S F O L L O W S
Short separation
bed is a major
disadvantage of
HPTLC
A limited number
of samples per
plate can be
tested.
Sometimes silica
gel is present
during detection.

12. TLC VS HPTLC

13. THANK YOU